Meselson–Stahl experiment Pour 14Nπ»4 πΆπ Nutrient medium Take a petri plate 14Nπ» 4 πΆπ − Ammonium chloride containing petri plate 14Nπ» 4 πΆπ − Ammonium chloride, Nitrogen isotope: 14N Allowing the Adding one E.coli bacteria in 14Nπ» πΆπ − Ammonium chloride 4 Nutrient medium bacteria to grow Splitting of DNA With Helicase First generation Bacteria Each template synthesizes new strand First generation Splitting of DNA Splitting of DNA Second generation Second generation bacteria Second generation bacteria starts synthesizing heavy 15N Which is present in medium Transferred to 15Nπ»4 πΆπ Nutrient medium Splitting Of DNA 15Nπ» 4 πΆπ − Ammonium chloride, Nitrogen isotope: 15N Process of DNA synthesizing heavy Template strand from 15Nπ»4 πΆπ βπππ£π¦ 14Nπ» 4 πΆπ 14Nπ» 4 πΆπ πππβπ‘ 15Nπ» 4 πΆπ βπππ£π¦ πππβπ‘ 14Nπ» 4 πΆπ 15Nπ» 4 πΆπ 14Nπ» 4 πΆπ πππβπ‘ βπππ£π¦ πππβπ‘ 14Nπ» 4 πΆπ 14Nπ» 4 πΆπ πππβπ‘ πππβπ‘ Third generation bacteria with one strand carrying parental DNA template Another strand With heavy DNA Template( Hybrid DNA)
