Enzyme properties
1. The effect of temperature on enzyme activity.
Temperature oC
10
15
20
25
30
35
40
45
50
55
60
Reaction rate
1.0
1.5
2.5
3.5
5.5
7.5
9.5
8.5
7.0
4.0
0.0
a. Draw a line graph of the results of the effects of temperature on a typical
enzyme reaction rate. Use a smooth hand drawn line to join the points
b. What is the best temperature for this enzyme to work?(try and be quite
accurate)
c. Most enzymes work at this temperature in humans- why do you think this is?
2. The effect of pH on enzyme activity.
pH
1.0
1.5
2.0
2.5
3.0
3.5
Reaction rate
8.5
10.0
8.5
7.0
5.5
3.0
d. Draw a line graph of the results of the effects of pH on a the enzyme pepsin’s
reaction rate. Use a smooth hand drawn line to join the points
e. What is the best pH for this enzyme to work?(try and be quite accurate)
f. Where is this enzyme found?
g. Why do you think it works best at such an acidic pH?