Name:
Class:
Date:
Refer to the diagram above and rearrange the statements in order of how
they occur.
These bacteria are then cultured, allowing them to reproduce asexually.
Some bacteria will hopefully take a plasmid into their cells.
Next, the plasmid is cut open using the same type of restriction enzyme.
The insulin gene is then joined into the plasmid using DNA ligase.
Bacteria are then mixed with the plasmids in a solution.
The human insulin gene is cut out using restriction enzymes.
The bacteria produces insulin which will be extracted and purified.
The diagram below shows the production of human growth hormone (hGH) gene
using genetic engineering.
1.
Structure A is a circular DNA taken from a bacterium. Name and explain its
importance in this process.
2.
Name the following enzymes:
• Enzyme X:
• Enzyme Y:
3.
Explain why it is important for the human growth hormone gene to be cut by the
same enzyme as the circular DNA.
4.
Explain how come the circular DNA carrying the hGH gene enter the bacterium.
5.
State the term for bacteria that contains hGH gene in this process.
6.
Define the term “vector” and identify the vector in this process.
Name:
Class:
Date:
Refer to the diagram above and rearrange the statements in order of how
they occur.
6
These bacteria are then cultured, allowing them to reproduce asexually.
5
Some bacteria will hopefully take a plasmid into their cells.
2
Next, the plasmid is cut open using the same type of restriction enzyme.
3
The insulin gene is then joined into the plasmid using DNA ligase.
4
Bacteria are then mixed with the plasmids in a solution.
1
The human insulin gene is cut out using restriction enzymes.
7
The bacteria produces insulin which will be extracted and purified.
The diagram below shows the production of human growth hormone (hGH) gene
using genetic engineering.
1.
Structure A is a circular DNA taken from a bacterium. Name and explain its
importance in this process.
Plasmid. It acts as a vector to transfer the gene of one organism another.
2.
Name the following enzymes:
• Enzyme X: Restriction enzyme
• Enzyme Y: DNA ligase
3.
Explain why it is important for the human growth hormone gene to be cut by the
same enzyme as the circular DNA.
To produce complementary sticky ends in the hGH gene and plasmid so that
they can be recombined perfectly.
4.
Explain how the circular DNA carrying the hGH gene enters the bacterium.
Using heat/ electric shock.
5.
State the term for bacteria that contains hGH gene in this process.
Transgenic bacteria
6.
Define the term “vector” and identify the vector in this process.
A vector is an agent that can carry the gene or genes of one organism to
another. The vector is the plasmid.
Name:
Class:
Date:
Genetic Engineering
Order the images and text to show how bacteria could be
genetically engineered to produce human insulin:
image
image
image
text
text
text
image
image
text
text
image
image
image
text
text
text
Genetic Engineering
Order the images and text to show how bacteria
could be genetically engineered to produce human
insulin:
A sample is taken
and spread on agar
jelly which contains
an antibiotic
Some bacteria will
hopefully take a
plasmid into their
cells
The gene is then
joined into the
plasmid using
another enzyme
The insulin gene is
cut out using
restriction enzymes
These bacteria are
then cultured,
allowing them to
reproduce asexually
Next, the plasmid is
cut open using the
same type of
restriction enzyme
Bacteria are then
mixed with the
plasmids in a
solution
Bacteria that survive
the antibiotic are
further cultured to
make insulin, which
will be extracted
Genetic Engineering
Order the images and text to show how bacteria
could be genetically engineered to produce human
insulin:
The insulin gene is
cut out using
restriction enzymes
Bacteria that survive
the antibiotic are
further cultured to
make insulin, which
will be extracted
The gene is then
joined into the
plasmid using
another enzyme
Next, the plasmid is
cut open using the
same type of
restriction enzyme
Bacteria are then
mixed with the
plasmids in a
solution
Some bacteria will
hopefully take a
plasmid into their
cells
A sample is taken
and spread on agar
jelly which contains
an antibiotic
These bacteria are
then cultured,
allowing them to
reproduce asexually
Genetic Engineering
Order the images and text to show how bacteria
could be genetically engineered to produce human
insulin:
The insulin gene is
cut out using
restriction enzymes
Bacteria that survive
the antibiotic are
further cultured to
make insulin, which
will be extracted
The gene is then
joined into the
plasmid using
another enzyme
Next, the plasmid is
cut open using the
same type of
restriction enzyme
Bacteria are then
mixed with the
plasmids in a
solution
Some bacteria will
hopefully take a
plasmid into their
cells
A sample is taken
and spread on agar
jelly which contains
an antibiotic
These bacteria are
then cultured,
allowing them to
reproduce asexually