SUPPLEMENTARY INFORMATI 7376189144ON
Adipogenic activity of 2-ethylhexyl diphenyl phosphate via peroxisome
proliferator-activated receptor γ pathway
Weijie Suna, Xiaoyu Duana, Hao Chena, Lianying Zhanga,b, Hongwen Suna 
a
MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of
Environmental Science and Engineering, Nankai University, Tianjin 300350, China
b
School of Environmental Science and Safety Engineering, Tianjin University of
Technology, Tianjin 300384, China.
 E-mail: sunhongwen@nankai.edu.cn (H. W. Sun). lianyingzh@yahoo.com (L. Y. Zhang)
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Table S1. Primers Used for RT-qPCR
Gene
Primer sequences
Sequences (5'to 3’)
FABP4
Forward TTCGATGAAATCACCGCAGAC
Reverse GCTCATGCCCTTTCATAAACTC
Lpl
Forward GCCCAGCAACATTATCCAGT
Reverse GGTCAGACTTCCTGTTACGC
Adip
Forward CCCAAGGGAACTTGTGCAGGTTGGATG
Reverse GTTGGTATCATGGTAGAGAAGAAAGCC
Fasn
Forward GATTAGCCTAAGACTGAAGCAT
Reverse CTGCTCCCTTGAGTCAGTAAA
β-actin
Forward ACACCCCAGCCATGTACG
Reverse TGGTGGTGAAGCTGTAGCC
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Fig S1. Effects of EHDPP and Rosi on the HEK-293T cell viability. HEK-293T cell were transiently
transfected with pBIND-PPARγ-LBD and pGL4.35[luc2P/9XGAL4UAS/Hygro] using Lipofectamine
2000. The cells were then treated with DMSO (0.5%), EHDPP (0.1~10 μM), or Rosi (1 μM) for 24 h
and the cell viability was measured by CCK-8 assay. The data are expressed as means ± SEM (n=4).
The results are presented as fold-change relative to the DMSO vehicle.
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Fig S2. Effects of EHDPP on 3T3-L1 cell viability. 3T3-L1 cells were exposed to DMSO (0.5%),
EHDPP (0.1~10 μM) for 10 days. The data are expressed as means ± SEM (n=4). The results are
presented as fold-change relative to the DMSO vehicle.
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