BSMT2-01 | Parasitology Introduction to Parasitology Amapola Puaso, RMT, MSMT November 6, 2019 First Semester A.Y. 2019-2020 Preliminary Exam 1-1 OUTLINE I. WHAT IS PARASITOLOGY A. Different Types of Host B. Classification of Parasites C. Types of Relationship Between Host and Parasite D. Modes of Transmission E. Classification of Microorganisms F. Different Portals of Entry of Parasites G. Diagnosis II. EPIDEMIOLOGICAL TERMS A. Epidemiology B. Epidemic C. Endemic D. Pandemic III. TEST QUESTIONS REFERENCES 1. Amapola Puaso, RMT, MSMT’s ppt 2. Recording 3. Zeibig, E. (2012). Clinical Parasitology 5. Paratenic host – harbors the infective stage of a parasite 6. Incidental host – infected person is not necessary for the parasite but they need them for survival and development Aka accidental host Host other than the normal one that is harboring a parasite 7. Transport host – host responsible for transferring a parasite from one location to another 8. Carrier – Parasite-harboring host that is not exhibiting any clinical symptoms but can infect others B. Classification of Parasites 1. According to habitat Ectoparasites – those found outside of the host Endoparasites – those found within the host 2. According to effect in host Pathogenic – the parasite is able to set up a disease process in man Non-pathogenic – the parasite is not able to set up a disease process in man Aka “commensals” I. WHAT IS PARASITOLOGY It is an area of biology concerned with the phenomenon of dependence of one living organism on another. It is a study of a particular relationship among certain species. Parasite – any organism that lives in or on the body of another organism (host) for survival for food and shelter Parasites alter their antigenic makeup so that the host will not recognize the modified parasites as foreign, and thus the initiation of an immune response does not occur. Host – any organism that harbors the parasite The primary function of the host is to carry on the parasite’s life cycle Parasitism – relationship wherein one derives the benefits and cause harm to the host C. Types Of Relationship Between Host And Parasite A. Different Types of Host 1. Intermediate host - harbors the larval or asexual stages of the parasites Host in which the larval asexual/juvenile phase of parasite development occurs example: snail, cat, dogs, plants, sheep 2. Definitive host – aka the final host It harbors the adult of the sexually mature stages of a parasite Host in which the adult sexual phase of parasite development occurs Examples: mosquito (malaria is the parasite), cat, dog 3. Reservoir host – aka the definitive host It harbors the parasite in nature, they can serve as the source of infection Host harboring parasites that are parasitic for humans and from which humans may become infected 4. Vector host – usually responsible for the infection of the vertebrate host Examples: mosquito (dengue: aedes; malaria: anopheles), tick, fly (can carry amoeba) When a parasite infects a host, symbiosis results. This newly-formed relationship may develop into commensalism, mutualism, or parasitism. 1. Obligatory – those that cannot survive without a host 2. Facultative – those that can survive without a host Parasite that is capable of existing independently of a host Aka free-living parasites Can survive in soil (for years) then may infect accidental hosts 3. Intermittent/temporary/periodic – so-called because they would simply visit the host during feeding time 4. Incidental/wandering/aberrant/erratic – a parasite that established itself in a host in which it does not usually live 5. Pseudoparasite – an object that is mistaken for a parasite Examples: pollen grains, vegetable fibers D. Modes of Transmission Mode of transmission – means whereby a parasite gains entry into an unsuspecting host 1. Soil transmitted – those that require further development in the soil before they become infective 2. Snail transmitted – requires further development in the body of a snail before they become infective The snail serves as the intermediate host, and the human will be the definitive host 3. Arthropod transmitted – requires further development in an arthropod/insect 4. Food-animal transmitted – requires further development in the flesh of animal that man utilizes as food 5. Contact transmitted – are readily infective BSMT 2019 – FESTIN A | Page 1 of 2 BSMT2-01 1-1 E. Different Portals of Entry of Parasites 1. Oral Entry – ingestion Ingestion of contaminated food or drink Examples: Embryonated eggs, protozoan cyst, metacercaria 2. Skin – through skin penetration Entry via drilling through the skin Examples: Filariform, Cercaria, arthropod 3. Sexual – Trichomonas vaginalis, Giardia lamblia, Entamoeba histolytica 4. Transplacental – mother to child, vertical transmission Example: Toxoplasma gondii 5. Intranasal – through breathing Example: Naegleria species 6. Transmammary – Strongyloides stercoralis 7. Airborne – Enterobious vermicularis F. Diagnosis (Specimen Requirement) Stool – ova, cyst, scolex Urine – Trichomonas vaginalis, Schistosoma haematobium Sputum – Paragonimus westermani, nematode larvae Blood – malaria, filarial worms Tissue biopsy – Trichinella spiralis, interstinal amoebiasis Orifice swab – Trichomonas vaginalis, Enterobius vermicularis, Taenia infection Originally, Enterobius and Taenia are only found in stool but due to improper washing, they can be transmitted to vagina. II. EPIDEMIOLOGICAL TERMS Epidemiology – the study of the occurrence and prevalence of disease, often applied to the study of the manner of spread of contagious disease Epidemic – sudden increase of infection; outbreak H1N1, Zika Virus, Ebola, SARS Endemic – certain infection is present constantly in low numbers, constantly present area Malaria (in Palawan), elephantiasis (in Sorsogon) Pandemic – refers to disease which spreads to several countries and affects a large number of people Malaria, HIV, TB, Hepa-B III. TEST QUESTIONS Test 1 1. Which of the following are key discoveries that contributed to current knowledge about parasites? a. Consistent status quo preservation of samples b. Techniques that indicate only the presence or absence of parasites c. Modifications of traditional parasite identification technique d. Decrease in parasite incidence because of global travel 2. Which of the ff people may be at risk for contracting a parasitic infection? a. A toddler who attends an all-day pre-school or day care center b. A 25-yr-old man who lives on his own in an apartment complex c. A 37-yr-old South American refugee d. More than one of these _____ (specify) INTRODUCTION TO PARASITOLOGY 3. The primary function of a host in a parasite-host relationship is to: a. Carry on the parasite’s life cycle b. Provide immunologic protection for the host c. Carry on the host’s life cycle d. Provide a food source for the host 4. Which of the ff key pieces of information may be extracted from the portion of a parasite’s life cycle that occurs outside the body? a. Parasitic disease symptoms and disease processes b. Epidemiology and prevention and control measures c. Appropriate parasite diagnosis methodologies d. Selection of appropriate antiparasitic medication 5. Which of the ff groups of symptoms represents those most commonly observed in parasitic infections? a. Diarrhea, abdominal cramping and anemia b. Enlargement of the spleen, fever and chills c. Skin lesions, abdominal pain, and diarrhea d. Abdominal cramping, abdominal pain, and diarrhea 6. Which of the ff represent examples of available treatment therapies to combat parasitic infections? a. Regulated exercise plan b. Change in diet c. Avoidance of vitamin supplements 7. Which of the ff are examples of possible parasite prevention and control measures? a. Avoiding the use of insecticides b. Practicing unprotected sex c. Practicing proper sanitation practices 8. Which of the ff specimen type is most often submitted for parasite study? a. Blood b. Sputum c. Urine d. Stool 9. Which of the ff correctly represents the three major groups of clinically significant parasites? a. Protozoa—worms; metazoan—single-celled parasites; arthropods—insects and their allies b. Protozoa—insects and their allies; metazoan—worms; arthropods—single-celled parasites c. Protozoa—single-celled parasites; metazoan—worms; arthropods—insects and their allies d. Protozoa—single-celled parasites; metazoan— insects and their allies; arthropods—worms Test 2 1. The form of a parasite that enters a host 2. Two organisms of different spp living together 3. The official units of parasite measurement 4. A parasite that cannot survive outside its host 5. An insect that transports a parasite from an infected host to an uninfected host 6. A parasite that lives on the outside surface of its host 7. Parasite-harboring host that is not affected by its presence but can shed the parasite and infect others 8. A destructive process that has characteristic symptoms 9. Association of 2 different spp of organisms that is beneficial to one but neutral to the other 10. A host responsible for transferring a parasite from one location to another Answers: Test 1: 1. C, 2. D(A+C,), 3. A, 4. A, 5. D (but I think all choices are correct), 6. B, 7. C, 8. D, 9. C Test 2: 1. Infective stage, 2. Symbiosis, 3. Microns, 4. Obligatory parasite, 5. Vector, 6. Ectoparasite, 7. Carrier, 8. Disease, 9. Commensalism, 10. Transport host BSMT 2019 – FESTIN A | Page 2 of 2