Cells: Basic Units of Life Eukaryotic Cell In this lesson In your book 1. Eukaryotic cells and compartmentalisation Pag. 5 2. Cell Structure Overview Pag. 14-20 3. Differences between animal and plant cells Pag. 5-6 Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 2 Vocabulary - 1 English Italian Nucleus Nucleo Nucleolus Nucleolo Chromatin Cromatina Chromosomes Cromosomi Nuclear envelope Membrana nucleare Nuclear pores Pori nucleari Endoplasmic reticulum rough / smooth Reticolo endoplasmatico ruvido / liscio Golgi apparatus (body) Apparato di Golgi Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 3 Eukaryotic cells and compartmentalisation • Eukaryotic cells contain organelles (such as nucleus). • An organelle is a intracellular, membrane-bound structure that performs a specific functions for the cell. o Eukaryotic cells can carry out more functions than prokaryotic cells. o Eukaryotic cells can have greater cell specificity than prokaryotic cells. Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 4 Cell Specialization Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 5 Warm Up! Video: The Cell is Like a City Worksheet – Cell City Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 6 Cell Structures Overview Organelle Organelle Nucleus Vesicles and vacuoles Nucleolus Centrioles Endoplasmic reticulum (ER): smooth & rough Other cellular structures: Golgi body Plasma membrane Ribosomes Cell wall Mitochondria Cytoplasm Cloroplasts Cytoskeleton Lysosomes Cilia Peroxisomes Flagella Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 7 Cells gotta work to live! • What jobs do cells have to do? • make proteins • proteins control every cell function • make energy • for daily life • for growth • make more cells • growth • repair • renewal Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 8 Cell structure 1 – Building Proteins Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 9 The Nucleus - 1 Structure o Surrounded by a double membrane (nuclear envelope). o The outer membrane is continuous with the endoplasmic reticulum. o The nuclear envelope contains “holes” called nuclear pores. o They allow movement of materials between nucleus and cytoplasm. Function o Contains the hereditary material (deoxyribonucleic acid – DNA), coding for the synthesis of proteins in the cytoplasm. Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 10 The Nucleus - 2 Inside of the nucleus: o The DNA can be organized in two ways: o Between cell divisions: a mass of DNA not condensed (chromatin). o During cell division: DNA condensed in structures called chromosomes (visible). o Nucleolus: a dense DNA region of the nucleus where the rRNA (ribosomal rna) and ribosomes are made. Prof. Vincenzi Chiara Chromatin During cell division: Chromosomes CAMBRIDGE BIOLOGY 11 The Endoplasmic Reticulum - 1 Structure o System of membranes running through the cytoplasm like a network. o Membranes are organized in flattened, enclose compartments or tube-like structures known as sacs. o The membranes of the ER are continuous with the outer nuclear membrane. Function o It acts as an intracellular highway that transports molecules from one part of the cell to another. Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY Micrograph of rough endoplasmic reticulum network around the nucleus (shown in lower right-hand side of the picture). Dark small circles in the network are mitochondria. 12 The Endoplasmic Reticulum - 2 There are two types, Smooth ER and Rough ER. Rough ER: o“rough” because covered with many ribosomes (visible as black dots). oFunctions: transporting proteins synthesized on the ribosomes in small sacs (vescicles) to Golgi apparatus. Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 13 The Endoplasmic Reticulum - 3 Smooth ER: oFew or no ribosomes. oInterconnetted with the rough ER. oFunctions: Rough RE o synthesis of carbohydrates, lipids and steroids (such as phospholipids, cholesterol and reproductive hormones); o detoxification of medications and poisons; o alcohol metabolism; o storage of calcium ions. Prof. Vincenzi Chiara Smooth RE CAMBRIDGE BIOLOGY 14 Ribosomes • Small spherical organelles that are responsible for building protein molecules. • The do not have a membrane! • Some ribosomes are free in the cytosol others are attached to the ROUGH ER. Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 15 The Golgi Apparatus Structure o A system of FLATTENED, membranous sacs that extend from the endoplasmic reticulum. o It receives and releases vesicles from the ER to other parts of the cell. Function o It collects, modifies and moves molecules from the endoplasmic reticulum to their destination (inside or outside the cell). Video: The Golgi Apparatus Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 16 vesicle vesicle protein p oein rt 1) Nucleolus makes ribosomes 2) Ribosome travels through rough ER making proteins 3) Golgi Body sorts & packages the protein 4) Vesicle releases the protein from the cell Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY P L A S M A M E M B R A N E 17 1 Minute Activity Ribosome 1. Which organelle builds proteins? ____________ Nucleus 2. Stores DNA? ____________ Nucleolus 3. Produces ribosomes? ___________ Smooth ER 4. Creates fats and breaks down toxins? ___________ Golgi apparatus 5. Packages and releases proteins from a cell? __________ Cell membrane 6. Allows material to enter/exit a cell? ______________ Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 18 1 Minute Activity Place the following steps of protein creation in order from start to finish… A. Golgi body packages and exports the finished proteins in a vesicle B. Ribosomes travel along the rough ER and create proteins C. Nucleolus makes ribosomes 4. 3. 1. D. Ribosomes exit the nucleus 2. Prof. Vincenzi Chiara CAMBRIDGE BIOLOGY 19