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BSC-Thesis-Project-Proposal

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VIETNAM NATIONAL UNIVERSITY
INTERNATIONAL UNIVERSITY
School of Biotechnology
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BSc THESIS PROJECT PROPOSAL
Student’s name: Tran Tri Tam
ID: BTBTIU14202
Department: Biotechnology
I. PROJECT GENERAL INFORMATION
1. NAME OF STUDY:
Application of synergic effect for preparation of plant growth promotor
Oligoalginate directly from brown seaweed for safe vegetable hydroponic.
2. FIELD OF STUDY:
Biotechnology
3. SUPPERVISORS:
Associate Professor Ph.D Le Quang Luan
4. HOST INSTITUTE
Saigon Hydroponic Corporation (290/198 No Trang Long, Binh Thanh District, Ho
Chi Minh City) and Biotechnology Center of Ho Chi Minh City (No. 2374, Highway 1,
Quarter 2, Trung My Tay Ward, District 12, Ho Chi Minh City).
5. TIME (from …to…)
3 months, from 1/3/2019 to 1/6/2019
6. BUDGET ESTIMATION
10 million VND
Thesis proposal
II.
PROJECT CONTENTS
1. AIM
Determine the molecular weight and effective concentration of manufactured
oligoalginate preparations for the growth of vegetables (Green mustard and Nato lettuce)
grown by hydroponic method.
2. BACKGROUND
2.1. LITERATURE REVIEW.
Vegetables are familiar and indispensable food for Vietnamese families.
However large-scale production has not met the conditions of food safety and
hygiene such as using fertilizers and pesticides exceeding the prescribed level, without a
reasonable isolation time.
Currently the application of hydroponic method is a new step in Vietnamese
agriculture: this method of farming does not utilize soil, does not have the weed invasion;
does not use pesticides or herbicides; and can improve productivity and clean products
uniformly; This method also is easy to do and environmentally friendly.
Figure 1: Hydroponic system.(Source: Saigon
Hydroponic Corporation)
In brown seaweed (Sagassum) containing Alginic compound is an acidic
polysaccharide, soluble in water. Sodium Alginate is a salt of Alginic and Sodium, there
are also compounds such as Calcium Alginate, Alginate Ammonium.
Oligoalginate prepare by degraded alginate compounds with molecular weights of
7−26, 40−77, or 11−26 kDa were obtained by γ irradiation, hydrogen peroxide (5%
H2O2) treatment, or a combination treatment involving ionizing radiation and H2O2.
“The 14 kDa oligoalginate, prepared by the combined method, promoted the
growth of mustard greens and lettuce at an optimal concentration of 75 mg/L.”
“Effect of the concentration: The 14 kDa oligoalginate was found to optimally
promote the growth of the tested vegetables. Mustard greens treated with oligoalginate
displayed a significant increase in the root length and fresh biomass over the range of
50−100 mg/L, whereas the shoot height increased over the range of 75−100 mg/L. The
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Thesis proposal
greatest promotion of shoot height (8% increase), root length (26% increase), and fresh
biomass (44% increase) was obtained using 75 mg/L oligoalginate”
“Effect of Molecular weight, the greatest stimulation of vegetables was obtained
by treatment with 14 kDa oligoalginate. Relative to the control, the presence of
oligoalginate increased the mustard greens shoot height, root length, and fresh biomass by
14, 35, and 56%, respectively. The lettuce shoot height, root length, and fresh biomass
were increased by 30, 21, and 42%, respectively.”
Benefits of including Oligoalginate in hydroponic cultivation: Oligoalginate has
been shown to be a bioactive substance for plants, so increase the efficiency of safe
vegetable cultivation by hydroponic method.
2.2. WHY THIS PROJECT NEED TO BE CARRIED OUT
Determine the level of promoting growth and development (fresh biomass, high
tree height, root length and dry matter content) of oligoalginate active in molecular weight
and different concentrations on green spinach and Nato lettuce grown hydroponic.
2.3. THE CONTRIBUTION OF THIS PROJECT INTO SCIENCE
“Application of synergic effect for preparation of plant growth promotor
oligoalginate directly from brown seaweed.” made to contribute improve the process of
growing safe vegetables by hydroponics by applying organic compounds of natural
origin.
III. CONTENTS
3.1. HYPOTHESIS
H0: Application of synergic effect is not useful for preparation of plant
growth promotor oligoalginate directly from brown seaweed.
H1: Application of synergic effect is useful for preparation of plant growth
promotor oligoalginate directly from brown seaweed
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Thesis proposal
3.2. PROCESS OF PROJECT
(Flowchart to show the process of the project is required)
Part I: Preparation of Oligoalginates solution.
Part II: Preparation of hydroponic system and plants.
Part III:Applying Oligoalginates solution to hydroponic system.
3.2. DETAIL CONTENTS:
3.2.1. Part I
Material: Oligoalginate 14 kDa made at doeses of 25mg/L, 50mg/L,75mg/L,100mg/L.
Oligoalginate 7 kDa and 21 kDa made at doeses of 75mg/L.
Methods: The oligoalginates were prepared by γ-rays irradiation of swollen brown
seaweed containing H2O2. The addition of H2O2 to a swollen brown seaweed aiming to
reducing required irradiation dose for degradation of alginate to achieve growth-enhancing
oligoalginates.
3.2.2. Part II
Material: hydroponic vertical rigs, plants of green mustard and Nato lettuce, nutrition of
Saigon Thuy Canh Corp.
Methods: Each treatment consists four tubes contaning total fourty plants.
Preparation of seven treatments for green mustard and Nato lettuce.
3.2.3. Part III
Material: Oligo alginate solution, hydroponic vertical rigs, plants of green mustard and
Natto lettuce, nutrition of Saigon Thuy Canh Corp.
Methods:
1: Control: Growth plants in hydroponic without using Oligo alginate.
2: Growth plants in hydroponic supplemented with Oligo alginate 14 kDa at 25mg/L.
3: Growth plants in hydroponic supplemented with Oligo alginate 14 kDa at 50mg/L.
4: Growth plants in hydroponic supplemented with Oligo alginate 14 kDa at 75mg/L.
5: Growth plants in hydroponic supplemented with Oligo alginate 14 kDa at 100mg/l.
6: Growth plants in hydroponic supplemented with Oligo alginate 7 kDa at 75mg/L.
7: Growth plants in hydroponic supplemented with Oligo alginate 21 kDa at 75mg/L.
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Thesis proposal
Analysis: ANOVA, analysis of variances Mw, weight-average molecular weight
3.3. REFERENCES
1. Authors, Title, year, journal, vol, page
“Preparation of Oligo alginate Plant Growth Promoter by γ Irradiation of Alginate Solution
Containing Hydrogen Peroxide” Associate Professor PhD Le Quang Luan in 2012, page
1739,1740,1741.
IV. PROGRESS AND EXPECTED RESULTS
Contents
Expected results
Time
Part I
Preparation of Oligo alginates solution
10 days
Preparation of Oligo alginates solution
10 days
Preparation of Oligo alginates solution
10 days
Part II
Preparation of hydroponic and plants.
14 days
Preparation of hydroponic and plants.
14 days
Preparation of hydroponic and plants.
14 days
Part III
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Thesis proposal
Control: Growth plants in hydroponic no use plants of green mustard and Nato 30 days
lettuce growth normal.
Oligo alginate
6 treatments applying Oligo alginate in green Green mustard growth more than 30 days
Plants of control condition
spinach
6 treatments applying Oligo alginate in Nato Nato lettuce growth more than 30 days
plants of control condition
lettuce
V. SUPERVISOR’S APPROVAL
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Signature:................................................... ............................................. Date: .......................................................
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