AU Biology Lab
Lab Report
Name: Khojiakbarkhuja Erkinkhujaev G-7
Date: 10.10.2019
Animal cell. Buccal epithelial cell.
Aim: In this episode of lab class the main purpose was to observe a buccal epithelial cell as an
animal cell under an electron microscope in order to visualize the structure of the cell.
Materials:
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Glass microscope slides.
Plastic cover slips.
Paper towels or tissue.
Methylene Blue solution (0.5-1%).
Plastic pipette or dropper.
Sterile, individually packed cotton swabs.
Procedure:
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A clean cotton swab was taken and scrapped gently the inside of the mouth.
The cotton swab was smeared on the center of the microscope slide for 2-3 sec.
A drop of methylene blue solution was added.
Any excess solution was removed by allowing a paper towel to touch one side of
the coverslip.
5. The slide was placed on the microscope, with 4x or 10x objective in position and
the cell was found.
Results: Under the microscope I was able to find a buccal epithelial cell taken from the inside of
my mouth. The cell was smeared with the color of blue since we used a drop of methylene blue
solution. It should be noted that of the all components of the cell, a nucleus was very well
distinguished as it received methylene blue most. The reason for this is that the nucleus usually
contains a number of negatively charged molecules, namely DNA and RNA.
Discussion / Conclusion: Overall, the experiment we conducted gave me the result as I
expected. The cell we obtained from the mouth turned into the blue color after we add a drop of
methylene blue solution to it. Because the cytoplasm, especially the nucleus, of the cell contains
a lot of different negatively charged molecules, such as proteins, molecules of DNA and RNA.
The reason is that methylene blue stains negatively charged molecules in the cell.