What If There Are More Than Two Factor Levels? • Chapter 3 • Comparing more that two factor levels…the analysis of variance • ANOVA decomposition of total variability • Statistical testing & analysis • Checking assumptions, model validity • Post-ANOVA testing of means ANOVA_EXAMPLE P60 Brainerd 1 What If There Are More Than Two Factor Levels? • The t-test does not directly apply • There are lots of practical situations where there are either more than two levels of interest, or there are several factors of simultaneous interest • The analysis of variance (ANOVA) is the appropriate analysis “engine” for these types of experiments – Chapter 3, textbook • The ANOVA was developed by Fisher in the early 1920s, and initially applied to agricultural experiments • Used extensively today for industrial experiments ANOVA_EXAMPLE P60 Brainerd 2 An Example (See pg. 60) • Consider an investigation into the formulation of a new “synthetic” fiber that will be used to make cloth for shirts • The response variable is tensile strength • The experimenter wants to determine the “best” level of cotton (in wt %) to combine with the synthetics • Cotton content can vary between 10 – 40 wt %; some nonlinearity in the response is anticipated • The experimenter chooses 5 levels of cotton “content”; 15, 20, 25, 30, and 35 wt % • The experiment is replicated 5 times – runs made in random order ANOVA_EXAMPLE P60 Brainerd 3 ANOVA: Design of Experiments Chapter 3 •A A productdevelopment development engineer engineer isisinterested interested inininvestigating investigating the thetensile tensile A product product development engineer is interested in investigating strength of a strength new synthetic will be used to that makewill men’s the tensile of afiber newthat synthetic fiber beshirts. used The to engineer knows from previous experience that the strength is affected by the make men’s shirts. The engineer knows from previous •weight The engineer percent knows of cotton from used previous in the blend experience of materials that thefor strength the fiber. is affected experience that theofstrength is affected byofthe weight percent of Furthermore, by the weight he percent suspects cotton that increasing used in the theblend cotton content materials will forincrease the fiber. the cotton ininitially. the blend materials the fiber. strength,used at least Heof also knows thatfor cotton content Furthermore, should range •between aboutthat 10 he suspects percent and that40 increasing percent ifthe the cotton final cloth content to will have increase other heFurthermore, suspects increasing the cotton content willis increase the quality the strength, characteristics least initially. initially. that are He desired. also knows The engineer that cotton decides content to test should strength, atatleast specimens range between at five about levels 10 of percent cottonand weight 40 percent percent: if the 15 percent, final cloth 20 ispercent, to have25 percent, other quality 30 percent, characteristics and 35 percent. that are desired. •The engineer decides to test specimens at five levels of cotton weight percent: 15 percent, 20 percent, 25 percent, 30 percent, and 35 percent. He also decides to test five specimens at each level of cotton content. ANOVA_EXAMPLE P60 Brainerd 4 ANOVA: Design of Experiments Chapter 3 H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different Tensile Strength EXAMPLE PROBLEM A single-factor experiment with a = 5 levels of the factor and n = 5 replicates. The 25 runs should be made in random order. 15 20 25 30 35 ANOVA_EXAMPLE P60 Brainerd Cotton % 5 ANOVA: Design of Experiments Chapter 3 H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different Tensile Strength ONE FROM EACH 15 20 25 30 35 Can we determine which is better? ANOVA_EXAMPLE P60 Brainerd Cotton % 6 ANOVA: Design of Experiments Chapter 3 H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different REPLICATION Tensile Strength 15 20 25 30 35 Cotton % What does replication provide? ANOVA_EXAMPLE P60 Brainerd 7 ANOVA: Design of Experiments Chapter 3 H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different REPLICATION Effect Tensile Strength If the sample mean is used to estimate the effect of a factor in the experiment, then replication permits the experimenter to obtain a more precise estimate of this effect. 15 20 25 30 35 What else does replication provide? ANOVA_EXAMPLE P60 Brainerd Cotton % 8 ANOVA: Design of Experiments Chapter 3 H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different REPLICATION Effect Error Tensile Strength Allows the experimenter to obtain an estimate of the 2 experimental error. This 2 estimate if error becomes a xi of measurement basic unit for determining whether observed differences in the data are really statistically different. Cotton % σ σ = n 15 20 25 30 35 What assumption does the error estimate depend upon? ANOVA_EXAMPLE P60 Brainerd 9 ANOVA: Design of Experiments Chapter 3 H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different REPLICATION Effect Error RANDOMIZATION Tensile Strength Cotton % 15 20 25 30 35 ANOVA_EXAMPLE P60 Brainerd Both the allocation of the experimental material and the order in which the individual runs or trials of the experiment are to be performed are randomly determined. 10 ANOVA: Design of Experiments Chapter 3 Need to randomize run order COTTON% Experimental Run # 15 20 25 30 35 1 6 11 16 21 2 7 12 17 22 3 8 13 18 23 ANOVA_EXAMPLE P60 Brainerd 4 9 14 19 24 5 10 15 20 25 11 ANOVA: Design of Experiments Chapter 3 Test Seq Run # % 1 8 20 2 18 30 3 10 20 4 23 35 5 17 30 * * * 25 3 15 RANDOMIZE RUNS ANOVA_EXAMPLE P60 Brainerd 12 ANOVA: Design of Experiments Chapter 3 H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different REPLICATION Effect Error RANDOMIZATION Tensile Strength Cotton % 15 20 25 30 35 What if the measurements varied widely because of human operators? ANOVA_EXAMPLE P60 Brainerd 13 ANOVA: Design of Experiments Chapter 3 • PUSHUP EXAMPLE – Test if one can do push ups better in the morning or afternoon. 20 DATA POINTS – Select 40 people at random Is PM really better than AM? AM PM ANOVA_EXAMPLE P60 Brainerd 14 ANOVA: Design of Experiments Chapter 3 • • • What if the PM group of 20 was in better shape then the AM group of 20? What if the test was conducted on a Monday morning? What if different people counted the push ups between AM and PM? Is PM really better than AM? AM PM ANOVA_EXAMPLE P60 Brainerd 15 ANOVA: Design of Experiments Chapter 3 Controllable Factors Input PROCESS Output Uncontrollable Factors ANOVA_EXAMPLE P60 Brainerd 16 ANOVA: Design of Experiments Chapter 3 • BLOCKING – Used to limit the uncontrollable factors – Therefore increase precision • PUSH UP EXAMPLE – Paired Data = BLOCKING – Have same person do AM and PM – You are investigating AM vs PM not which group can do more pushups. – Randomly Sort experiment by Days of the Week and have one grader ANOVA_EXAMPLE P60 Brainerd 17 THE THREE PRINCIPLES of Experimental Design H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different Tensile Strength REPLICATION RANDOMIZATION BLOCKING A block is a portion of the experimental material that should be more homogeneous than the entire set of material. 15 20 25 30 35 ANOVA_EXAMPLE P60 Brainerd Cotton % 18 Analysis of Variance (ANOVA) H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different Variation Between Samples Tensile Strength Variation Within Samples 15 20 25 30 35 ANOVA_EXAMPLE P60 Brainerd Cotton % 19 Analysis of Variance (ANOVA) When very different Tensile Strength Between Sample Variation Large Within Sample Variation Small 15 20 25 30 35 ANOVA_EXAMPLE P60 Brainerd Cotton % 20 Analysis of Variance (ANOVA) When near equal Tensile Strength 15 20 25 30 35 Cotton % Between Sample Variation near equal to Within Sample Variation ANOVA_EXAMPLE P60 Brainerd 21 Analysis of Variance (ANOVA) Between Sample Variation TEST STAT = Within Sample Variation F-TEST REQUIRED ASSUMPTION All data is normal with equal variance EXTENSION OF TWO SAMPLE POOLED t ANOVA_EXAMPLE P60 Brainerd 22 Analysis of Variance (ANOVA) SETUP (i = factor; j = replicate) Replicates Level 1 2 3 * i xi , j Mean SD 1 2 3 * j 11 21 31 * i1 12 22 32 * i2 13 23 33 * i3 * * * * * 1j 1* 2j 2* 3j 3* * ij ** = observed value i th level, jth measurement ANOVA_EXAMPLE P60 Brainerd 23 Analysis of Variance (ANOVA) COTTON EXAMPLE Replicates Level 1 2 3 4 5 1 2 3 4 5 7 12 14 19 7 7 17 18 25 10 15 12 18 22 11 11 18 19 19 15 9 18 19 23 11 Mean SD 9.80 3.35 3.13 2.07 2.61 2.86 15.40 17.60 21.60 10.80 15.04 xi• = sample mean i th level x•• = Grand Mean ANOVA_EXAMPLE P60 Brainerd 24 Analysis of Variance (ANOVA) I = # factors and J = # replicates Mean Square Treatment ≡ Between Sample Variation J 2 MSTr = ( X i • − X •• ) ∑ I −1 i Mean Square Error ≡ Within Sample Variation S + S + S + ....S MSE = I 2 1 2 2 2 3 ANOVA_EXAMPLE P60 Brainerd 2 I 25 Analysis of Variance (ANOVA) H 0 : µ1 = µ 2 = µ 3 = ... = µ i H a : At least one µ i is different Between Sample Variation TEST STAT = Within Sample Variation MSTr f = MSE Fα , I −1, I ( J −1) If H 0 true E ( MSTr ) = E ( MSE ) = σ 2 If H 0 false E ( MSTr ) > E ( MSE ) = σ 2 ANOVA_EXAMPLE P60 Brainerd 26 Anova Chapter 3 (See pg. 62) • • Does changing the cotton weight percent change the mean tensile strength? Is there an optimum level for cotton content? ANOVA_EXAMPLE P60 Brainerd 27 The Analysis of Variance SST = SSTreatments + SS E • A large value of SSTreatments reflects large differences in treatment means • A small value of SSTreatments likely indicates no differences in treatment means • Formal statistical hypotheses are: H 0 : µ1 = µ 2 = L = µ a H1 : At least one mean is different ANOVA_EXAMPLE P60 Brainerd 28 The Analysis of Variance • While sums of squares cannot be directly compared to test the hypothesis of equal means, mean squares can be compared. ( MS = Estimates of variances) • A mean square is a sum of squares divided by its degrees of freedom: dfTotal = dfTreatments + df Error an − 1 = a − 1 + a ( n − 1) SSTreatments SS E MSTreatments = , MS E = a −1 a ( n − 1) • If the treatment means are equal, the treatment and error mean squares will be (theoretically) equal. • If treatment means differ, the treatment mean square will be larger than the error mean square. ANOVA_EXAMPLE P60 Brainerd 29 The Analysis of Variance is Summarized in a Table • • • Computing…see text, pp 70 – 73 The reference distribution for F0 is the Fa-1, a(n-1) distribution Reject the null hypothesis (equal treatment means) if F0 > Fα ,a −1,a ( n −1) ANOVA_EXAMPLE P60 Brainerd 30 Analysis of Variance (ANOVA): Excel Analysis: ANOVA Single Factor Setup data in EXCEL Spreadsheet in columns as: 15% Cotton 20% Cotton Tensile Strength Tensile Strength lb/in2 lb/in2 25% Cotton 30% Cotton 35% Cotton Tensile Tensile Tensile Strength lb/in2 Strength lb/in2 Strength lb/in2 7 12 14 19 7 7 17 18 25 10 15 12 18 22 11 11 18 19 19 15 9 18 19 23 11 ANOVA_EXAMPLE P60 Brainerd 31 Analysis of Variance (ANOVA): Excel Analysis: ANOVA Single Factor EXCEL Data Analysis: ANOVA Single Factor ANOVA_EXAMPLE P60 Brainerd 32 Analysis of Variance (ANOVA): EXCEL Data Analysis: ANOVA Single Factor ANOVA_EXAMPLE P60 Brainerd 33 Analysis of Variance (ANOVA): EXCEL Data Analysis: ANOVA Single Factor Output EXCEL Anova: Single Factor SUMMARY Groups 15% Cotton Tensile Strength lb/in2 20% Cotton Tensile Strength lb/in2 25% Cotton Tensile Strength lb/in2 30% Cotton Tensile Strength lb/in2 35% Cotton Tensile Strength lb/in2 Count Sum Average Variance 5 49 9.8 11.2 5 77 15.4 9.8 5 88 17.6 4.3 5 108 21.6 6.8 5 54 10.8 8.2 ANOVA_EXAMPLE P60 Brainerd 34 Analysis of Variance (ANOVA): EXCEL Data Analysis: ANOVA Single Factor Output H 0 : µ1 = µ 2 = µ 3 = ... = µ i If H 0 true H a : At least one µ i is different E ( MSTr ) = E ( MSE ) = σ 2 TEST STAT = If H 0 false MSTr f = MSE E ( MSTr ) > E ( MSE ) = σ 2 ANOVA Source of Variation Between Groups Within Groups Total SS Between Sample Variation Within Sample Variation df MS F 475.76 4 118.94 161.2 20 8.06 636.96 24 P-value F crit 14.75682 9.128E-06 P-Value: Probability of wrongly rejecting the Null ANOVA_EXAMPLE P60 Brainerd 2.866081 Fα , I −1, I ( J −1) 35 The Reference Distribution: ANOVA_EXAMPLE P60 Brainerd 36 Analysis of Variance (ANOVA): EXCEL Data Analysis: ANOVA Manual calculations 15% 20% 25% 30% 35% Cotton Cotton Cotton Cotton Cotton Tensile Tensile Tensile Tensile Tensile Strength Strength Strength Strength Strength (Σxi) SUMj (SUM)SQj SUM SQj (Σxj) (Σxj)2 Σ(xj2) 7 12 14 19 7 59 3481 799 7 17 18 25 10 77 5929 1387 15 12 18 22 11 78 6084 1298 11 18 19 19 15 9 18 19 23 11 82 80 6724 6400 1392 1416 Σ(Σxj) Σ((Σxj)2) 49 77 88 108 54 Σ(Σxi) 376 Σ(Σ(xij2)) Σ(Σxij) (Σxi)2 Σ(xi2) 2401 525 5929 1225 7744 1566 11664 2360 2916 616 Σ((Σxi2) 2 Σ(Σ(xi )) 30654 376 Σ(Σ(xj2)) 28618 6292 6292 ANOVA_EXAMPLE P60 Brainerd 37 Analysis of Variance (ANOVA): EXCEL Data Analysis: ANOVA Manual calculations Manual calculations Source of Variation SS - sum of squares Between Groups =(r( ΣΣxi )-(ΣΣxi) )/n Within Groups Total 2 Error 2 SS SS - sum of squares Calculation df MS = SS/df estimate of sigma 475.76 as = [(5 x30654) (376^2)]/25 4 118.94 161.2 =(n( ΣΣxij2)-(ΣΣxij)2)/n 636.96 20 as = [(25 x 6292) (376^2)]/25 24 ANOVA_EXAMPLE P60 Brainerd 8.06 F statistic = MSBG/MSwithin G as = 118.94/8.06 = 14.76 38 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor General Factorial Design STEP 1 Press continue ... ANOVA_EXAMPLE P60 Brainerd 39 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor General Factorial Design STEP 2 Press continue ... ANOVA_EXAMPLE P60 Brainerd 40 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor General Factorial Design STEP 3 Define # replicates Press continue ... ANOVA_EXAMPLE P60 Brainerd 41 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor General Factorial Design STEP 4 # and Name response Press continue ... ANOVA_EXAMPLE P60 Brainerd 42 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 5 Run experiment in the defined random order and measure/input responses Press continue ... ANOVA_EXAMPLE P60 Brainerd 43 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 6 Design Expert Analysis ANOVA_EXAMPLE P60 Brainerd 44 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 7 Design Expert Analysis ANOVA ANOVA_EXAMPLE P60 Brainerd 45 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 8 Design Expert Analysis ANOVA_EXAMPLE P60 Brainerd 46 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 9 Design Expert Analysis Effects M for Model e for error ANOVA_EXAMPLE P60 Brainerd 47 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA same table as before in EXCEL ANOVA_EXAMPLE P60 Brainerd 48 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Terms Model: Terms estimating factor effects. For 2-level factorials: those that "fall off" the normal probability line of the effects plot. Sum of Squares: Total of the sum of squares for the terms in the model, as reported in the Effects List for factorials and on the Model screen for RSM, MIX and Crossed designs. DF: Degrees of freedom for the model. It is the number of model terms, including the intercept, minus one. Mean Square: Estimate of the model variance, calculated by the model sum of squares divided by model degrees of freedom. F Value: Test for comparing model variance with residual (error) variance. If the variances are close to the same, the ratio will be close to one and it is less likely that any of the factors have a significant effect on the response. Calculated by Model Mean Square divided by Residual Mean Square. Probe > F: Probability of seeing the observed F value if the null hypothesis is true (there is no factor effect). Small probability values call for rejection of the null hypothesis. The probability equals the proportion of the area under the curve of the F-distribution that lies beyond the observed F value. The F distribution itself is determined by the degrees of freedom associated with the variances being compared. (In "plain English", if the Probe>F value is very small (less than 0.05) then the terms in the model have a significant effect on the response.) ANOVA_EXAMPLE P60 Brainerd 49 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis Information in Help System ANOVA Terms Pure Error: Amount of variation in the response in replicated design points. Sum of Squares: Pure error sum of squares from replicated points. DF: The amount of information available from replicated points. Mean Square: Estimate of pure error variance. Cor Total: Totals of all information corrected for the mean. Sum of Squares: Sum of the squared deviations of each point from the mean. DF: Total degrees of freedom for the experiment, minus one for the mean. ANOVA_EXAMPLE P60 Brainerd 50 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA ANOVA_EXAMPLE P60 Brainerd 51 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Information in Help System ANOVA Terms Next you see a collection of summary statistics for the model: Std Dev: (Root MSE) Square root of the residual mean square. Consider this to be an estimate of the standard deviation associated with the experiment. Mean: Overall average of all the response data. C.V.: Coefficient of Variation, the standard deviation expressed as a percentage of the mean. Calculated by dividing the Std Dev by the Mean and multiplying by 100. PRESS: Predicted Residual Error Sum of Squares – A measure of how the model fits each point in the design. The PRESS is computed by first predicting where each point should be from a model that contains all other points except the one in question. The squared residuals (difference between actual and predicted values) are then summed. R-Squared: A measure of the amount of variation around the mean explained by the model. 1-(SSresidual / (SSmodel + SSresidual)) ANOVA_EXAMPLE P60 Brainerd 52 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Information in Help System ANOVA Terms Summary statistics for the model continued: Adj R-Squared: A measure of the amount of variation around the mean explained by the model, adjusted for the number of terms in the model. The adjusted R-squared decreases as the number of terms in the model increases if those additional terms don’t add value to the model. 1-((SSresidual / DFresidual) / ((SSmodel + SSresidual) / (DFmodel + DFresidual))) Pred R-Squared: A measure of the amount of variation in new data explained by the model. 1-(PRESS / (SStotal-SSblock) The predicted r-squared and the adjusted r-squared should be within 0.20 of each other. Otherwise there may be a problem with either the data or the model. Look for outliers, consider transformations, or consider a different order polynomial. ANOVA_EXAMPLE P60 Brainerd 53 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Information in Help System ANOVA Terms Summary statistics for the model continued: ANOVA_EXAMPLE P60 Brainerd 54 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Scroll down ANOVA_EXAMPLE P60 Brainerd 55 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Scroll down ANOVA_EXAMPLE P60 Brainerd 56 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Scroll down ANOVA_EXAMPLE P60 Brainerd 57 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Scroll down ANOVA_EXAMPLE P60 Brainerd 58 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Scroll down ANOVA_EXAMPLE P60 Brainerd 59 Model Adequacy Checking in the ANOVA Text reference, Section 3-4, pg. 76 • • • • • Checking assumptions is important Normality Constant variance Independence Have we fit the right model? ANOVA_EXAMPLE P60 Brainerd 60 Model Adequacy Checking in the ANOVA STEP 10 Design Expert Analysis ANOVA Diagnostics Residuals • Examination of residuals (see text, Sec. 3-4, pg. 76) eij = yij − yˆij = yij − yi. • • • Design-Expert generates the residuals Residual plots are very useful Normal probability plot of residuals ANOVA_EXAMPLE P60 Brainerd 61 Analysis of Variance (ANOVA): STAT EASE Design Expert: ANOVA Single Factor STEP 10 Design Expert Analysis ANOVA Model Graphs ANOVA_EXAMPLE P60 Brainerd 62 Other Important Residual Plots 5.2 5.2 2.95 2.95 R es iduals R es iduals 2 2 0.7 2 2 0.7 -1.55 -1.55 2 2 2 -3.8 -3.8 9.80 12.75 15.70 18.65 21.60 1 4 7 10 13 16 19 22 25 R un N um ber Predicted ANOVA_EXAMPLE P60 Brainerd 63 Post-ANOVA Comparison of Means • The analysis of variance tests the hypothesis of equal treatment means • Assume that residual analysis is satisfactory • If that hypothesis is rejected, we don’t know which specific means are different • Determining which specific means differ following an ANOVA is called the multiple comparisons problem • There are lots of ways to do this…see text, Section 3-5, pg. 86 • We will use pairwise t-tests on means…sometimes called Fisher’s Least Significant Difference (or Fisher’s LSD) Method ANOVA_EXAMPLE P60 Brainerd 64 Graphical Comparison of Means Text, pg. 89 ANOVA_EXAMPLE P60 Brainerd 65 For the Case of Quantitative Factors, a Regression Model is often Useful Response:Strength ANOVA for Response Surface Cubic Model Analysis of variance table [Partial sum of squares] Sum of Mean F Source Squares DF Square Value Prob > F Model 441.81 3 147.27 15.85 < 0.0001 A 90.84 1 90.84 9.78 0.0051 A2 343.21 1 343.21 36.93 < 0.0001 A3 64.98 1 64.98 6.99 0.0152 Residual 195.15 21 9.29 Lack of Fit 33.95 1 33.95 4.21 0.0535 Pure Error 161.20 20 8.06 Cor Total 636.96 24 Coefficient Factor Estimate Intercept 19.47 A-Cotton % 8.10 A2 -8.86 A3 -7.60 Standard 95% CI 95% CI DF Error Low High 1 0.95 17.49 21.44 1 2.59 2.71 13.49 1 1.46 -11.89 -5.83 1 2.87 -13.58 -1.62 ANOVA_EXAMPLE P60 Brainerd VIF 9.03 1.00 9.03 66 The Regression Model Strength = +62.61143 -9.01143* Cotton Weight % +0.48143 * Cotton Weight %^2 -7.60000E003 * Cotton Weight %^3 25 % 20.5 2 2 Strength Final Equation in Terms of Actual Factors: 2 16 2 11.5 This is an empirical model of the experimental results 2 7 2 2 15.00 20.00 25.00 30.00 35.00 A: C otton Weight % ANOVA_EXAMPLE P60 Brainerd 67