Chapter 1 Introduction: Themes in the Study of Life PowerPoint Lectures for Biology, Seventh Edition Neil Campbell and Jane Reece Lectures by Chris Romero Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Overview: What is Biology? • Biology is the scientific study of life • People who study it are "biologists“ • Biologists are moving closer to understanding: – How a single cell develops into an organism – How plants convert sunlight to chemical energy – How the human mind works – How living things interact in communities – How life’s diversity evolved from the first microbes Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Life’s basic characteristic is a high degree of order • Each level of biological organization has emergent properties Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Characteristics of life 1. The ordinate structures All organisms consist of the basic unit: cell – Basic life unit: Cell, (organelles and biological macromolecules) – Biosphere: cell, tissue, organ, organ system, organism (individual), population, community and ecosystem – Without such ordinate structures, there is no life Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Characteristics of life – Unicellular (Prokaryotic) organisms • Single cell – Multicellular (Eukaryotic) organisms • Many cells organized to form tissues, organs, and organ systems Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Characteristics of life 2. Metabolism processes Living organisms carry out metabolism in which they use and transform energy. – Chemical reactions and energy transformations – Growth and repair of cells, and conversion of energy – Regulation of metabolic processes maintains homeostasis Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Characteristics of life 3. All living things maintain stable internal conditions, or homeostasis. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Characteristics of life 4. Growth – Increase in the size of cells, the number of cells, or both Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Characteristics of life 5. Living organisms reproduce – Reproduction in plants and animals takes place sexually, resulting in the production of offspring. – Reproduction can also occur via asexual propagation Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Characteristics of life 6. Heredity: • All living things possess a genetic system based on DNA and transmit characteristics from parent to offspring in a process called heredity Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Characteristics of life 7. Adaptation (Response to the environment) – Organisms evolve to better survive – Adaptations to the environment • Characteristics that enhance an organism’s ability to survive in a particular environment • May be structural, physiological, behavioral, or a combination Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.3 Order Response to the environment Evolutionary adaptation Reproduction Regulation Energy processing Growth and development Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.3g Order Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A Hierarchy of Biological Organization 1. Biosphere: all environments on Earth 2. Ecosystem: all living and nonliving things in a particular area 3. Community: all organisms in an ecosystem 4. Population: all individuals of a species in a particular area 5. Organism: an individual living thing Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A Hierarchy of Biological Organization (continued) 6. Organ and organ systems: specialized body parts made up of tissues 7. Tissue: a group of similar cells 8. Cell: life’s fundamental unit of structure and function 9. Organelle: a structural component of a cell 10. Molecule: a chemical structure consisting of atoms Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The biosphere Ecosystems Organelles 1 µm Cell Cells Atoms 10 µm Communities Molecules Tissues 50 µm Populations PowerPoint Lectures for Organisms Biology, Seventh Edition Neil Campbell and Jane Reece Lectures by Chris Romero Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Organs and organ systems Figure 1.4a The biosphere Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.4b Ecosystems Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.4c Communities Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.4d Populations Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.4e Organisms Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.4f Organs and organ systems Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.4g 50 m Tissues Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.4h Cell Cells Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 10 m Figure 1.4i Chloroplast 1 m Organelles Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.4j Atoms Chlorophyll molecule Molecules Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 1.1: Biologists explore life from the microscopic to the global scale • The study of life extends from molecules and cells to the entire living planet • Biological organization is based on a hierarchy of structural levels Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A Hierarchy of Biological Organization Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Populational level Organism Population Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Community Ecosystem 29 A Closer Look at Ecosystems • Each organism interacts with its environment • Both organism and environment affect each other Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Ecosystem Dynamics • The dynamics of an ecosystem include two major processes (functions): – Cycling of nutrients, in which materials acquired by plants eventually return to the soil – The flow of energy from sunlight to producers to consumers Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Figure 1.5 Sunlight Leaves absorb light energy from the sun. CO2 Leaves take in carbon dioxide from the air and release oxygen. O2 Cycling of chemical nutrients Leaves fall to the ground and are decomposed by organisms that return minerals to the soil. Water and minerals in the soil are taken up by the tree through its roots. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Animals eat leaves and fruit from the tree. Cycling of nutrients: (Nitrogen cycle) Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The phosphorus cycle Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The functions of Ecosystem •Energy flow •Ecosystems depend on continuous input of energy Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Ecosystem • Organisms can neither create energy nor use it with complete efficiency • During every energy transition, some is lost to the environment as heat Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Energy Conversion • Activities of life require work • Work depends on sources of energy • Energy exchange between an organism and environment often involves energy transformations • In transformations, some energy is lost as heat • Energy flows through an ecosystem, usually entering as light and exiting as heat Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 1-4 Sunlight Ecosystem Producers (plants and other photosynthetic organisms) Heat Chemical energy Consumers (including animals) Heat A Closer Look at Cells • The cell is the lowest level of organization that can perform all activities of life • The ability of cells to divide is the basis of all reproduction, growth, and repair of multicellular organisms Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Theme: The Cell Is an Organism’s Basic Unit of Structure and Function • All cells – Are enclosed by a membrane – Use DNA as their genetic information Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings © 2011 Pearson Education, Inc. Two Main Forms of Cells • Characteristics shared by all cells: – Enclosed by a membrane – Use DNA as genetic information • Two main forms of cells: – Eukaryotic: divided into organelles; DNA in nucleus – Prokaryotic: lack organelles; DNA not separated in a nucleus Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • A eukaryotic cell has membrane-enclosed organelles, the largest of which is usually the nucleus • By comparison, a prokaryotic cell is simpler and usually smaller, and does not contain a nucleus or other membrane-enclosed organelles Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings © 2011 Pearson Education, Inc. Figure 1.8 Prokaryotic cell Eukaryotic cell Membrane DNA (no nucleus) Membrane Cytoplasm Nucleus (membraneenclosed) Membraneenclosed organelles Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings DNA (throughout nucleus) 1 m Structure of a plant cell Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Structure of an animal cell Cells Basic unit of organismal organization; compartmentalize macromolecules and organelles Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Prokaryotes have a very simple architecture – They lack a nucleus and organelles Pilus Found in all prokaryotes Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Cell’s Heritable Information • Cells contain DNA, the heritable information that directs the cell’s activities • DNA is the substance of genes • Genes are the units of inheritance that transmit information from parents to offspring Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 1-6 Sperm cell Nuclei containing DNA Egg cell Fertilized egg with DNA from both parents Embryo’s cells With copies of inherited DNA Offspring with traits inherited from both parents Figure 1.11 Nucleus A C DNA Nucleotide T A T Cell A C C G T A G T A (a) DNA double helix (b) Single strand of DNA Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Each DNA molecule is made up of two long chains arranged in a double helix • Each link of a chain is one of four kinds of chemical building blocks called nucleotides and nicknamed A, G, C, and T Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings © 2011 Pearson Education, Inc. DNA molecule The DNA double helix • Each DNA molecule is made up of two long chains arranged in a double helix • Each link of a chain is one of four kinds of chemical building blocks called nucleotides Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Concept 1.2: Biological systems are much more than the sum of their parts • A system is a combination of components that form a more complex organization • System = a network of relationships among a group of parts, elements, or components that interact with and influence one another through the exchange of energy, matter, and/or information Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Emergent Properties of Systems • Emergent properties result from arrangements and interactions within systems • New properties emerge with each step upward in the hierarchy of biological order Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Emergent properties Properties of a whole system not evident in the system’s components “The whole is more than the sum of its parts.” A tree is an element of a forest, a sink for CO2, and habitat for birds. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Systems show several defining properties • Systems receive input, process it, and produce output. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Feedback loops: Negative feedback Feedback loop = a circular process whereby a system’s output serves as input to that same system •In a negative feedback loop, output acts as input that moves the system in the opposite direction. This compensation stabilizes the system Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Feedback loops: Positive feedback • In a positive feedback loop, output acts as input that moves the system further in the same direction. • This magnification of effects destabilizes the system. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Positive feedback • In a positive feedback loop, the output drives the system further toward one extreme. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • In negative feedback, the accumulation of a product slows down the process itself • In positive feedback (less common), the product speeds up its own production Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • Concept 1.3: Biologists explore life across its great diversity of species • Biologists have named about 1.8 million species • Estimates of total species range from 10 million to over 200 million Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Grouping Species: The Basic Idea • Taxonomy is the branch of biology that names and classifies species into a hierarchical order • Kingdoms and domains are the broadest units of classification Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Three Domains of Life • At the highest level, life is classified into three domains: – Bacteria (prokaryotes) – Archaea (prokaryotes) – Eukarya (eukaryotes) Eukaryotes include protists and the kingdoms Plantae, Fungi, and Animalia Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Three-Domain Classification • Archaea – Prokaryotesin cell walls • Bacteria – Prokaryotesin cell walls • Eukarya – all eukaryotes: protists, fungi, plants, animals Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Three-Domain Classification Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Protists Plants Fungi Animals First eukaryote Bacteria Archaea Stepped Art Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Fig. 23-4b, p. 489 Figure 1.15 2 m (b) Domain Archaea 2 m (a) Domain Bacteria (c) Domain Eukarya Kingdom Animalia 100 m Kingdom Plantae Protists Kingdom Fungi Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 1-15 Bacteria Archaea 4 µm 0.5 µm Protists Kingdom Fungi 100 µm Kingdom Plantae Kingdom Animalia Charles Darwin • The evolutionary view of life came into sharp focus in 1859, when Charles Darwin published On the Origin of Species by Natural Selection Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings • The Origin of Species articulated two main points: – Descent with modification (the view that contemporary species arose from a succession of ancestors) – Natural selection (a proposed mechanism for descent with modification) Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Natural Selection • Darwin inferred natural selection by connecting two observations: – Observation: Individual variation in heritable traits – Observation: Overpopulation and competition – Inference: Unequal reproductive success – Inference: Evolutionary adaptation Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 1-20 Population of organisms Overproduction and competition Hereditary variations Differences in reproductive success Evolution of adaptations in the population Darwin’s theory: variation is inherited Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Tree of Life • Many related organisms have similar features adapted for specific ways of life • Such kinships connect life’s unity and diversity to descent with modification • Natural selection eventually produces new species from ancestral species • Biologists often show evolutionary relationships in a treelike diagram Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Tree of Life Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Results of Cladistic Analyses Sometimes Run Counter to Classical Classification Schemes Which pair is more closely related? A lizard/crocodile or bird/crocodile? Cladistic analysis indicates that the bird/crocodile pair is more closely related. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Classification of Living Things • Taxomony is the science of identifying and classifying organisms according to specific criteria using these categories: Kingdom Phylum (Division) Class Order Family Genus Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Species Taxa • Hierarchical system of classification Domain Kingdom Phylum Class Order Family Genus Species Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Higher Categories • Taxonomists use a hierarchical system to classify organisms Information gets more and more general – Species spaghetti – Genus green – Family for – Order – Class – Phylum – Kingdom Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings over came Philip King • Concept 1.5: Biologists use various forms of inquiry to explore life • Inquiry is a search for information and explanation, often focusing on specific questions • The process of science blends two main processes of scientific inquiry: – Discovery science: describing nature – Hypothesis-based science: explaining nature Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Discovery Science • Discovery science describes nature through careful observation and data analysis • Examples of discovery science: – understanding cell structure – expanding databases of genomes Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Types of Data • Data are recorded observations • Two types of data: – Quantitative data: numerical measurements – Qualitative data: recorded descriptions Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Induction in Discovery Science • Inductive reasoning involves generalizing based on many specific observations Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings A Closer Look at Hypotheses in Scientific Inquiry • A scientific hypothesis must have two important qualities: – It must be testable – It must be falsifiable Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Scientific method • The scientific method involves systematic thought – Deductive reasoning draws conclusions from premises – Inductive reasoning begins with observations and draws conclusions or extrapolates Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Stage of a scientific method The scientific method involves 6 steps: 1. Observation: Find a question 2. Hypothesis 3. Predictions 4. testing: experiment 5. Control 6. Results and Conclusion Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 1. Observation • You might observe something unusual or something very common. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 2. Hypothesis • A hypothesis attempts to explain an observation. It is an educated guess • As questions are asked, scientist attempt to answer those questions by proposed explanations that answer the questions. • Those proposed explanations are called hypothesis. • Alternative hypotheses: If they have more than one guess about what they observe. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Hypothesis-Based Science • In science, inquiry usually involves proposing and testing hypotheses • Hypotheses are hypothetical explanations – Educated guess Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Role of Hypotheses in Inquiry • In science, a hypothesis is a tentative answer to a well-framed question • A hypothesis is an explanation on trial, making a prediction that can be tested Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Prediction: The “If…then” Logic of Hypothesis-Based Science • In deductive reasoning, the logic flows from the general to the specific • In inductive reasoning begins with observations and draws conclusions or extrapolates 1. Observation: Find a question – 2. Hypothesis – 3. Predictions – 4. testing: experiment – 5. Control – 6. Results and Conclusion • If a hypothesis is correct, then we can expect a particular outcome Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings LE 1-25a Observations Question PowerPoint Lectures for Biology, Seventh Edition Hypothesis #1: Neil Campbell and Jane Reece Dead batteries Lectures by Chris Romero Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Hypothesis #2: Burnt-out bulb LE 1-25b Hypothesis #1: Dead batteries Hypothesis #2: Burnt-out bulb Prediction: Replacing batteries will fix problem Prediction: Replacing bulb will fix problem Test prediction Test prediction PowerPoint Lectures for Biology, Seventh Edition Neil Campbell and Jane Reece Test falsifies hypothesis Lectures by Chris Romero Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Test does not falsify hypothesis 3. Prediction • The prediction states the expected results of the experiments based on the hypothesis. Predictions often take the form of an “if…then…” statement: “IF the hypothesis is correct, then the results of the experiment will be…” Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 4. Testing • Test the hypothesis by attempting to verify some of its predictions • We called the test of a hypothesis an experiment (Procedure, process) Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings How to perform the testing • Set up the variables – Independent variables – Dependent variables – Control variables • Control treatments (standard, or zero) • Levels of treatment • Replications Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Variables • The independent variable is what the investigator varies during the experiment. It is what the investigator thinks will affect the dependent variable. • The dependent variable is what the investigator measures (or counts or records). It is what the investigator thinks will be affected during the experiment. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 5. Controls: Designing Controlled Experiments • Controlled variables: we test one independent variable and keep the other factors are constant – Scientists do not control the experimental environment by keeping all variables constant • Control treatment: A control is a treatment in which the independent variable is either eliminated or set at a standard value. – Researchers usually “control” unwanted variables by using control groups to cancel their effects • Zero test Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Results: Figure 1. Relationship between plant weight and fertilizer content 12 Expected curve Actual curve Average weight of plants (kg) 10 8 6 4 2 0 0 1 2 3 4 Conentration of fertilizer (%) Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 5 6 7 Other graphs: Bar Chart, Pie chart -1 Mean FCO2 (mol m-2 s ) 10 9 Gap Non-gap 8 7 ** 6 ** ** 5 4 3 2 0 2 4 6 8 Years after harvesting Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings 10 1 2 3 4 6. Conclusion • The results either support or do not support the hypothesis. • As long as the experiment is good, whatever the results is same or different with the hypothesis, the results is valid Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Experimental design Completely randomized design (CRD) Tree diameters in response to Fertilization experiment Treatment: Concentration of Fertilization F50kg/ha F100kg/ha (year6) Total 4*5*=20 Tree diameters Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings F150kg/ha C0 In the fertilizer example • the control would be treatment in which no fertilizer is applied or some standard amount of fertilizer is applied. • This allows the scientist to be sure that the effect on the dependent variable is in fact due to the independent variable. Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings