2015 – 2016 Intel Philippine Science Fair Research Paper PART I: BACKGROUND INFORMATION 1. Student’s Name 2. School SAMBILE, Janine Anne M. Baguio City High School 4. School Address Cor. Governor Pack Road, Baguio City 5. Telephone/ Fax Number 6. Title of Project (074) 444 26 45 A Comparative study on the rate of fermentation of Guyabano juice (tetra pack fruit juice) and Apple juice (natural fruit juice) 7. Project Adviser/ Adult Sponsor Juliet Ilustre-Herreria 8. This year’s experiment began and ended (mo/day/year) (mo/day/year) 9. Where did you conduct your lab work? (check all that apply) [ ] Research Institution [ / ] School [ ] Field [ / ] Home 10. Name, Address & Phone no. of lab work site(s): PART II. RESEARCH PAPER (TYPED OR COMPUTER PRINTOUT – DO NOT BOOK BIND) As attachment to Part I, The research paper must include the following: a) Problem or question being addressed b) Objectives/Goals c) Method/Procedures (describe in detail) d) Results e) Conclusion/Recommendation f) Bibliography (List at least 3 major references from your library research). Can be reproduce IPSF – Form – B Republic of the Philippines DEPARTMENT OF EDUCATION U.L. Complex, Meralco Ave., Pasig City APPLICATION FORM – TEAM PROJECT INSTRUCTION: This form must be accomplished by all high school project leaders of team projects. Please TYPE or PRINT clearly. Region: CAR Please Check: Level: Cluster: Science Fair Division: Field of Study/Discipline: Microbiology 1. Name of Project Proponent [ ] Division [ ] Regional [ ] National []1 [ ]2 [ ] Life Sciences [ ] Physical Sciences (Please refer to the identified disciplines in the IPSF Guidelines) SAMBILE Surname JANINE ANNE First Name MERCADO Middle Name Surname First Name Middle Name Surname First Name Middle Name Member Member 2. Permanent Home Address Lot 2, Block 3, SLU-SVP, Bakakeng, Baguio City No. Street 3. Telephone/Fax No. 4. Sex 6. School/Address of School Telephone/Fax No. District Benguet City/Municipality FEMALE 5. Birth Date Province 09-16-00 Baguio City National High School – Cor. Gov. Pack Rd., Baguio City (074) 444 26 45 7. Year Level Fourth year A Comparative study on the rate of fermentation of Guyabano juice (tetra pack fruit juice) and Apple juice (natural fruit juice) 9. Attach one copy of accomplished OFFICIAL ABSTRACT FORM: (Not more than 250 words, one page only). 8. Title of Investigatory Project Janine Anne M. Sambile Printed Name & Signature of Project Proponent NOTED: Juliet Ilustre- Herreria Printed Name & Signature of Project Adviser/Adult Sponsor JACQUELINE G. LAMPAC, Ed.D Printed Name & Signature of Principal Or Head of S & T Department Date Submitted 2016 – 2017 INTEL PHILIPPINE SCIENCE FAIR RESEARCH PLAN Required for All Projects To be Submitted to IRB/SRC before the Experimentation Begins Name School Cluster Baguio City High School Category Fair Division Cor. Gov. Pack Rd., Baguio City School Address Title of Project Project Adviser The research plan must include the following: A) Problem/s B) Hypothesis/Objectives: C) Methods/ Procedure (Describe in Detail) D) BIBLIOGRAPHY (074) 444 26 45 Tel. No. IPSF OFFICIAL ABSTRACT FORM Category pick one only mark an X in box at right TITLE: NAME: SCHOOL: The purpose of this project is to come up with a substitute for antioxidant wherein the consumers will benefit both in economic and nutritional aspects. In this study, the expediency of bougainvillea (Bougainvillea spectabilis) leaves extract as antioxidant was tested. 300mg dried and fresh samples were collected and were soaked in ethanol for 48 hours and were macerated. Next, the extracts were filtered until all the extract passed through the filter paper. After this, it was distilled and evaporated until all the ethanol was separated from the extracts. The extracts were put in containers with peeled potatoes for observations. The results showed that the bougainvillea (Bougainvillea spectabilis) extract has the expediency to be a substitute for antioxidant. The results showed that bougainvillea (Bougainvillea spectabilis) leaves extract contains a number of alkaloids, phytosterols, phenolics and flavonoids which makes it effective as an antioxidant. Also, there is a significant difference of the different bougainvillea (Bougainvillea spectabilis) extracts and control since the computed F-value (17.79727599) is greater than the critical Fvalue (2.368270235) at alpha 0.05% level of significance. It was also identified that as the concentration of extract increases, its effectivity as antioxidant also increases as revealed by the absorbance reading at 517 nm wavelength and the percentage of freeradical inhibition but in the peeled potato test, it was an indirect relationship. The researcher also discovered that fresh bougainvillea (Bougainvillea spectabilis) leaves extract is more effective than dried bougainvillea leaves extract. Lastly, the best concentration of the bougainvillea extract is 1284.775 µg/mL. Behavioral and social science Biochemistry Botany Chemistry Computers Earth Sciences Engineering Environmental Sciences Mathematics Medicine and Health Microbiology Space Science Physics Zoology As a part of this research project, the student directly handle, manipulated, or interacted with (check ALL) that apply. Human Subjects Vertebrae Animals controlled pathogenic agents recombinant DNA substances human/animal tissue 2. Students independently performed all procedures as outlined in this abstract. 3. This project was conducted at regulated research institutions. 4. Is this project a continuation? 5. My display board includes photographs/visuals depictions of humans (other than myself). I/We certify that the above statements are correct and the information provided in the abstract is the result of one year’s research. I/We also attest that the above properly reflects my/our own work. ________________ Project Proponent Signature Date x IPSF – Form I – D CERTIFICATE OF PARENTAL CONSENT I who is the legal parent(s)/guardian(s) of with residence at do hereby Certify that I/We give our son/daughter to participate in the 2015 – 2016 Intel Philippines Science Fair, on under the following conditions: 1. Our child/ward will be chaperoned by his/her project adviser; a. The chaperone will see to the safety, behavior and physical upkeep of our child as far as humanly possible; b. Considering the above conditions and the benefits that our child will derive from his/her participation in this activity, and realizing that every precaution will be undertaken by the adviser/chaperone and your office, we the undersigned parent(s)/guardian(s) of the above name project investigator do hereby give our consent for him/her to the point of official debarkation. Name and Signature of Parent/Guardian Date Signed MEDICAL CERTIFICATE Date: This is to certify that of BAGUIO CITY NATIONAL HIGH SCHOOL has undergone physical examination and was found to be physically fit to participate in the 2015 – 2016 Intel Philippines Science Fair, on . REMARKS: JOCELYN M. DE JESUS Examining Physician License no. 72845 Hospital/Clinic Office: BCNHS Address: Gov. Pack Rd. IPSF Form 3 – B 2015 – 2016 Intel Philippines Science Fair SCIENTIFIC REVIEW OF IPSF PROJECTS Fair Division: [ ] Life Science Cluster: [ ]1 Category: [ ] Individual [ ] Physical Science [ ]2 [ ] Team Level: Venue: Date: Instruction: Please put a check (/) on appropriate box and write recommendations on space provided. Project Title: REMARKS 1. Completed forms, signature and dates (to be verified by IPSF Secretariat) [ ] complete [ ] incomplete 2. Evidence of use of reference materials [ ] complete [ ] incomplete 3. Evidence of proper laboratory supervision [ ] complete [ ] incomplete 4. Use of accepted research techniques [ ] Yes [ ] No [ ] Needs certification/data notebook 5. Use of pathogenic organisms, hazardous substances and devices/disposal of wastes [ ] Proper [ ] Improper 6. Status of Investigatory projects [ ] original concept of study (does not violate intellectual property rights) [ ] with innovation [ ] with violation found [ ] needs changes/improvement 7. Over – all evaluation of project [ ] Approved [ ] Disapproved CERTIFICATION This is to certify that the above project was reviewed by the Intel Philippines Science Fair Committee. SRC Member’s Printed Name Signature Date of Approval CERTIFICATION AS TEAM PROJECT This is to certify that the project submitted by ________________, (Team Leader) _____________________, and ___________ of Baguio City National High School (Member) (Member) (School) As official entry to 2015 – 2016 Intel Philippines Science Fair was conducted/experimented by the above mentioned three (3) students. ___________________ Printed Name and Signature of Adviser/Adult Sponsor NOTED: JACQUELINE G. LAMPAC, Ed.D Printed Name & Signature of Principal or Head of S & T Department Date Signed: 2015 – 2016 Intel Philippine Science Fair Research Paper PART I: BACKGROUND INFORMATION 2. Student’s Name 2. School 4. School Address Cor. Governor Pack Road, Baguio City 5. Telephone/ Fax Number (074) 444 26 45 6. Title of Project 7. Project Adviser/ Adult Sponsor 8. This year’s experiment began 06- 01- 15 and ended (mo/day/year) (mo/day/year) 9. W///here did you conduct your lab work? (check all that apply) [ ] Research Institution [ ] School [ ] Field [ ] Home 10. Name, Address & Phone no. of lab work site(s): PART II. RESEARCH PAPER (TYPED OR COMPUTER PRINTOUT – DO NOT BOOK BIND) As attachment to Part I, The research paper must include the following: a) Problem or question being addressed b) Objectives/Goals c) Method/Procedures (describe in detail) d) Results e) Conclusion/Recommendation f) Bibliography (List at least 3 major references from your library research). Can be reproduce IPSF – Form – B Republic of the Philippines DEPARTMENT OF EDUCATION U.L. Complex, Meralco Ave., Pasig City APPLICATION FORM –INDIVIDUAL PROJECT INSTRUCTION: This form must be accomplished by all high school investigators /project researchers of individual projects. Please TYPE or PRINT clearly. Region: CAR Please Check: Level: [ ] District Cluster: Science Fair Division: Field of Study/Discipline: 1. Name of Project Proponent 2. Permanent Home Address [ ] Division [ / ] Regional [ ] National [ ]1 [/]2 [ / ] Life Sciences [ ] Physical Sciences (Please refer to the identified disciplines in the IPSF Guidelines) Lim Surname Leandro First Name #9 Lower Malvar St. Trancoville No. Street 3. Telephone/Fax No. 442-6903 6. School/Address of School Telephone/Fax No. District 4. Sex Male Tan Middle Name Baguio City, Benguet City/Municipality 5. Birth Date Province July 31,2000 Baguio City National High School – Cor. Gov. Pack Rd., Baguio City (074) 444 26 45 8. Title of Investigatory Project 7. Year Level Grade 9 The Expediency of Bougainvillea (Bougainvillea spectabilis) Leaves Extract as Antioxidant 9. Attach one copy of accomplished OFFICIAL ABSTRACT FORM: (Not more than 250 words, one page only). Leandro T. Lim Printed Name & Signature of Project Proponent NOTED: Marilyn Degay Printed Name & Signature of Project Adviser/Adult Sponsor FLORENCE E. BALICTAN, Ed.D Printed Name & Signature of Principal Or Head of S & T Department Date Submitted CERTIFICATION OF INDIVIDUAL PROJECT This is to certify that the project submitted by of Baguio City National High School. (School) As official entry to 2015 – 2016 Intel Philippines Science Fair was conducted/experimented by the student mentioned above. ___ Printed Name and Signature of Adviser/Adult Sponsor NOTED: FLORENCE E. BALICTAN, Ed.D Printed Name & Signature of Principal or Head of S & T Department Date Signed: Can be produced IPSF-Form I-E CERTIFICATION AS OFFICIAL PROJECT ADVISER This is to certify that __ ___ is the Official Project Adviser / Adult (Name of Adviser) Sponsor of project entitled, __________________________________________________________________________________________ _________________________________________________________________________________________ as an official entry of _____Baguio City National High School___ to the 2015-2016 Intel Philippine (School) Science Fair. FLORENCE E. BALICTAN, Ed.D Printed Name & Signature of Principal or Head of S&T Department _______________ Date Signed