SIDC-FM-F-SOS-004
TRIAL RESULT
REVISION 00
(Effectivity Date: 05 Feb 2018)
TRIAL REF NO: 2018-008
DATE: September 12, 2018 – December 19, 2018
I.
TITLE:
COMPARISON OF DIFFERENT TYPES OF ANTI-AMMONIA ON
PERFORMANCE OF PIGS AND AMMONIA EMISSION ON STARTER-GROWER STAGE
(Enthera® of Philchema, Dinase® of Realvet, Puregro® of B&V/DSM and Effective
Microorganism of SIDC)
Trial Site:
SIDC Trial Farm
Sorosoro Ilaya, Batangas City
Dinase® is a high technology feed additive from Yucca schidigera plant. It is a naturally
concentrated extract obtained through a low temperature process that assures retention and
efficacy of natural ingredients.
Enthera® is a synergistic combination of probiotics, prebiotics and immune-stimulating factors
that improves feed efficiency and intake, enhance immune strength, controls diseases and
lessens farm wastes and odor.
PureGro® is a heat-stable microbial feed additive that contains microorganisms selected for
their ability to improve decomposition of stored manure. It reduces the solids in manure and
minimizes objectionable odor. It contains viable spores of carefully selected naturally occurring
microorganisms (Bacillus subtilis and Bacillus lichenformis).
Effective Microorganisms are cultures of naturally-occuring organisms that enables growth
of diverse microbes that are beneficial for use.
II.
•
•
OBJECTIVES:
To determine the effect of different anti-ammonia on performance of pigs and ammonia
emission on starter-grower stage
To evaluate profitability of different anti-ammonia on starter-grower diets.
III.
MATERIALS:
A total of one hundred fifty piglets (150) with about the same age will be distributed to five (5)
dietary treatments following a completely randomized design. Each treatment will be replicated
three (3) times with ten (10) piglets per replicate.
Dinase
Page 1 of 13
PRICE
DOSE
P656.00/kg
100 g/ton – S
ADD’L
COST/BAG
P3.28/bag – S
TOTAL
MATERIALS
540 grams
Enthera
Puregro
Effective Mircoorganism
P550/kg
P260/kg
P150/kg
120 g/ton – G
500 g/ton
500 g/ton
1000 g/ton
P3.94/bag – G
P13.75/bag
P6.50/bag
P7.50/bag
2.5 kgs
2.5 kgs
5 kgs
Treatment Groups are as follows (Not the real grouping on actual trial)
S. SIDC HSM & HGM w/o Dinase ® (Negative Control)
T. SIDC HSM & HGM with Dinase ® (Positive Control / Commercial Feeds)
U. SIDC HSM & HGM with Enthera ®
V. SIDC HSM & HGM with Puregro ®
W. SIDC HSM & HGM with Effective Microorganism
Estimated Feed Consumption for Starter Special Stage
Treatment
Group
Feed Type
SIDC HSM & HGM w/o Dinase® (Negative
Control)
SIDC HSM & HGM with Dinase® (Positive Control
/ Commercial Feeds)
SIDC HSM & HGM with Enthera ®
SIDC HSM & HGM with Puregro ®
SIDC HSM & HGM with Effective Microorganism
S
T
U
V
W
Number
of Pigs
ADFI
Per Head,
grams
Feeding
Days
Total Feed
Requirement
(bags)*
30
1.10
15
10 bags
30
1.10
15
10 bags
30
30
30
1.10
1.10
1.10
15
15
15
10 bags
10 bags
10 bags
Number
of Pigs
ADFI
Per Head,
grams
Feeding
Days
Total Feed
Requirement
(bags)*
30
1.35
30
25 bags
30
1.35
30
25 bags
30
30
30
1.35
1.35
1.35
30
30
30
25 bags
25 bags
25 bags
Number
of Pigs
ADFI
Per Head,
grams
Feeding
Days
Total Feed
Requirement
(bags)*
30
2.00
30
36 bags
30
2.00
30
36 bags
30
30
30
2.00
2.00
2.00
30
30
30
36 bags
36 bags
36 bags
Estimated Feed Consumption for Starter Stage
Treatment
Group
Feed Type
SIDC HSM & HGM w/o Dinase® (Negative
Control)
SIDC HSM & HGM with Dinase® (Positive Control
/ Commercial Feeds)
SIDC HSM & HGM with Enthera ®
SIDC HSM & HGM with Puregro®
SIDC HSM & HGM with Effective Microorganism
S
T
U
V
W
Estimated Feed Consumption for Grower Stage
Treatment
Group
S
T
U
V
W
Feed Type
SIDC HSM & HGM w/o Dinase® (Negative
Control)
SIDC HSM & HGM with Dinase® (Positive Control
/ Commercial Feeds)
SIDC HSM & HGM with Enthera ®
SIDC HSM & HGM with Puregro®
SIDC HSM & HGM with Effective Microorganism
Page 2 of 13
IV.
METHODOLOGY:
a. Upon arrival, randomly assign the piglets to different treatment groups.
b. Individually weigh the trial animals and record pig id numbers and initial weights.
c. The animals will be initially fed with Hog Starter Special Mash without anti-ammonia
as flushing for the period of 2 weeks.
d. After flushing, feed the animals using the assigned feed rations. Monitor and record
the quantity given and daily feed consumption.
e. Ammonia level will be measured using ammonia meter and ammonia strips every
Tuesday at 7 am and 3 pm for the period of 2 months, 1-month starter and 1-month
grower.
f. All incidences of morbidities and mortalities will be noted throughout the trial period.
1. In cases of culling or mortality, the current weight and feeds consumed by
the pig will be recorded.
2. Tentative diagnosis as well as the medications given will be noted.
g. Additional Notes / Suppliers accountabilities:
1. Expenses will be divided into 3 (SIDC, Philchema, B&V)
1. Cost of Statistical analysis will be conducted for the level of confidence
/ significance.
2. Any negative variance of the treatment group from the positive control
will be shouldered by the supplier.
3. Salary of the caretaker for the duration of the trial
4. Cost of ear tags
2. The suppliers are free to visit the trial, however, SIDC Feedmill should be
notified first before going to the farm.
3. Trial results of their own treatment group will be disclosed to the suppliers
and the rest of the treatment groups will be coded for confidentiality.
4. The suppliers reserve the right to publish the trial results and be used for
promotional activities.
DATA NEEDED:
a. Initial weights
b. Final weights
c. Daily Feed Consumption
d. Average Daily Feed Intake (actual value)
e. Feed Conversion Ratio
f. Cost efficiency
Page 3 of 13
g. ADG
h. Mortality and Morbidity, %
i. Ammonia level in ammonia meter
j. Ammonia level in ammonia strips
V.
RESULTS AND DISCUSSIONS:
a. % Culled / Mortality
% Culled / % Mortality
10.00%
6.67%
a
3.33% 3.33% 3.33%
b
0.00% 0.00%
0.00% 0.00%
0.00%
% Culled
% Mortality
Puregro
EM
Enthera
PC (Dinase)
NCONTROL
Reason for culling: rectal prolapse and excessive thumping
Reason for mortality: thumping
b. Weight Distribution
Initial Weight Distribution
120
100
18
80
23
60
23
40
17
10
6
7
7
10
15.1 to 20
20.1 to 25
21
20
0
1
0
2
10.1 to 15
Trial S
Trial T
Trial U
Trial V
Trial W
The initial weights of the trial animals ranged from 13.50 to 26.50 kgs.
Page 4 of 13
1
0
25.1 to 30
Final Weight Distribution
45
40
35
11
30
1
25
5
20
7
5
0
10
6
15
10
6
4
2
2
3
1
4
10
4
6
2
5
4
5
2
7
2
6
1
0
4
1
1
3
0
2
1
1
1
0
60.1 to 65 65.1 to 70 70.1 to 75 75.1 to 80 80.1 to 85 85.1 to 90 90.1 to 95 95.1 to
100
Trial S
Trial T
Trial U
Trial V
1
3
1
2
2
0
1
0
0
1
2
1
100.1 to 105.1 to 110.1 to
105
110
115
Trial W
The weight distribution of the trial animals after 98 days of feeding ranged from 63 to
123.50 kgs.
PUREGRO
# of heads
18
19
ABOVE NORMAL
NORMAL
7
5
6
4
STARTER
FINAL
BELOW NORMAL
For Puregro, the number of piglets in the normal weight increased by 1. The below normal
and above normal weight group decreased from 5 to 4 heads and 7 to 6 heads
respectively. The difference in the two groups were transferred to above normal weight
group with a recorded mortality of 1 head.
Page 5 of 13
EFFECTIVE MICROORGANISM
25
# of heads
24
ABOVE NORMAL
NORMAL
BELOW NORMAL
3
2
STARTER
FINAL
Using Effective Microorganism, the quantity of pigs in the below normal and above normal
weight group both decreased by 1, with 1 of which transferred to the normal weight group
and 1 recorded as mortality.
ENTHERA
23
# of heads
19
ABOVE NORMAL
NORMAL
BELOW NORMAL
4
3
4
STARTER
FINAL
In Enthera, the quantity of pigs in the normal weight group is decrease by 4 heads, while
the normal weight group maintained the number of heads. An increase by 1 head in the
below normal weight group was transferred from the normal weight group and the
remaining heads were recorded as culling and mortality.
DINASE
# of heads
24
Page 6 of 13
19
ABOVE NORMAL
NORMAL
3
6
2
STARTER
FINAL
BELOW NORMAL
For Dinase, the quantity of pigs from the normal weight group and above normal weight
group decreased by 5 heads and 1 head, respectively. 3 of which were transferred to the
below normal weight group and the rest were mortalities.
NEGATIVE CONTROL
# of heads
26
ABOVE NORMAL
17
NORMAL
9
BELOW NORMAL
4
3
1
STARTER
FINAL
For Negative Control, the number of heads in the above normal weight group and below
normal weight group decreased by 8 heads and 1 head, respectively. All of which were
transferred to the normal weight group.
c. Average Weights / Weight Gain
AVERAGE
WEIGHTS
ab
a
a
a
b
b
b
b
a
a
b
ab
a
b
b
Weight, kgs
b
Puregro
Initial Wt
19.60
Grower Wt
74.66
Final Wt
95.95
WEIGHT GAIN
76.35
EM
18.70
73.19
91.09
72.39
Enthera
18.62
72.17
93.25
74.63
PC (Dinase)
18.68
74.93
90.78
72.09
NCONTROL
19.88
70.85
89.30
69.42
The final weight is based on the 98 feeding days. With this, Puregro has the highest
weight gain with 76.35 kgs followed by Enthera with 74.63 kgs.
Page 7 of 13
d. ADG
ADG
a
0.779
0.780
0.760
0.740
0.720
0.700
0.680
0.660
0.762
b
b
a
ab
0.739
0.736
b
b
b
0.708
Final ADG
Puregro
EM
Enthera
PC (Dinase)
NCONTROL
Puregro got the highest ADG when weighed followed by Enthera.
e. FCR
FCR
2.69
2.63 2.62
2.62
2.58
2.51
a
2.52
2.57
a
2.51
a
2.45
b
FCR
Puregro
Corrected FCR
EM
Enthera
PC (Dinase)
NCONTROL
Puregro got the best FCR among treatment groups.
Page 8 of 13
f. Average Daily Feed Intake, kgs
ADFi
1.68
1.68
1.66
1.65
1.66
1.62
1.64
1.61
1.62
1.60
1.58
ADFi
Puregro
EM
Enthera
PC (Dinase)
NCONTROL
Dinase got the highest average daily feed intake followed by Puregro.
g. Average Feeds Consumed per Head, kgs
Ave Feeds Consumption/head, kgs
194.00
192.00
193.86
191.36
190.00
189.35
188.00
186.00
187.83
187.65
186.93
185.74
186.60
184.00
182.00
181.89
181.89
180.00
178.00
176.00
174.00
Average Feed Consumption
Ave Corrected Feed Consumption
Puregro
EM
Enthera
PC (Dinase)
NCONTROL
Dinase has the highest average feed consumption per head with 193.86 kgs equivalent
to 3.88 bags per head.
Excluding the mortalities and culled pigs, Dinase also got the highest average corrected
feed consumption per head with 189.35 kgs equivalent to 3.79 bags.
Page 9 of 13
h. Feed Cost per Bag, Php
Feed Cost Per Bag, Php
1,300.00
1,250.00
1,200.00
1,150.00
1,100.00
1,050.00
1,000.00
Starter Special
Starter
Grower
Puregro
1,275.76
1,132.22
1,108.81
EM
1,275.76
1,131.92
1,108.51
Enthera
1,275.76
1,138.17
1,114.76
PC (Dinase)
1,275.76
1,127.70
1,104.29
NCONTROL
1,275.76
1,124.42
1,101.01
i. Feed Cost per Head, Php
Feed Cost / Head
4300.00
4250.00
4200.00
4253.70
4257.20
4222.65
4189.79
4150.00
4100.00
4080.93
4050.00
4000.00
3950.00
Puregro
EM
Enthera
PC (Dinase)
NCONTROL
Dinase has the highest feed cost per head with P4257.20 with the highest average feed
consumption and still the highest on the corrected feed consumption.
Page 10 of 13
j. Feed Cost Efficiency, Php
Feed Cost Efficiency
59.19
60.00
59.00
58.00
57.00
56.00
55.00
54.00
53.00
58.78
57.88
56.99
55.37
Feed Cost Efficiency
Puregro
EM
Enthera
PC (Dinase)
NCONTROL
The most efficient in terms of feed cost is Puregro with only P55.37 feed cost to convert
1 kg weight gain.
k. Ammonia Levels
AMMONIA STRIP READING AM & PM
p=0.104
35.00
30.00
25.00
20.00
15.00
10.00
5.00
0.00
Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10
Puregro
EM
Enthera
Dinase
NC
With a ceiling value of 20 ppm which is the allowable limit set by the DENR, it would
appear that the negative control (NC) is consistently low than other treatments followed
by the effective microorganism (EM).
Page 11 of 13
AMMONIA METER READING AM & PM
35.00
30.00
25.00
20.00
15.00
10.00
5.00
0.00
Week 1
Week 2
Puregro
Week 3
EM
Week 4
Enthera
Week 5
Dinase
NC
To validate the result of the strips, ammonia level testing was done using ammonia meter
which also shows that the negative control is consistently low followed by the effective
microorganism as well.
Page 12 of 13