Physiology and Genetics SB0024 CHAPTER 4: GENE TECHNOLOGY 1. Define the following: (a) Polymerase chain reaction (1 mark) (b) Vector (1 mark) 2. List five tools needed in recombinant DNA technology. (1 mark) X Y Process Z Physiology and Genetics SB0024 3. (a) Name structures X and Y. (2 marks) (b) State advantages using structure Y in the process. (3 marks) (c) Name process Z and enzyme involved in this process. (2 marks) 4. Describe steps in recombinant DNA process in genetic engineering. (6 marks) 5. State and give functions of all enzymes involved in cloning process. (6 marks) 6. State and briefly explain about the method used in cells amplification. (6 marks)