Gluconeogenesis Gluconeogenesis and starch/glycogen synthesis • In animals, lactate formed anaerobically in muscles is converted to glucose in liver and kidney and stored as glycogen or released as blood glucose. • In plants, G3P product of photosynthesis is converted to starch and stored in chloroplasts or converted to glucose and sucrose and exported to other tissues for starch storage. • In some plant seeds, stored fats are converted to glucose and sucrose upon germination and used to make cell wall cellulose. Gluconeogenesis is the synthesis of glucose. Gluco neo genesis Standard free energies Go’ glycolysis neogenesis -16.7 -17.7 +1.7 -1.7 -14.2 -16.7 +23.8 +7.6 -23.8 -7.6 +6.3 -6.3 -18.9 +18.9 +4.4 -4.4 +1.8 -1.8 -31.4 -22.6 ________________ -74 -37.7 Gluco neo genesis Working free energies G’ glycolysis neogenesis -33.9 -5.1 -2.9 -18.9 -8.6 -0.2 +2.4 -1.3 +0.1 +0.8 +1.1 -22.6 -23.0 -75.8 -36.3 Gluconeogenesis starts in the mitochondrion ..and finishes up in the endoplasmic reticulum (to release glucose). + + + - Why control? Note possible futile cycle: - F1,6BP + H2O -> F6P + Pi F6P + ATP -> F1,6BP + ADP Net: ATP + H2O -> ADP + Pi + While animals can use many amino acids to make glucose, they cannot use acetyl-CoA from fatty acid metabolism. Why? Removing malate from the TCA cycle to make carbohydrates means that there will be a lack of OAA to form citrate. But plants and bacteria can convert acetyl-CoA to glucose using the glyoxylate cycle. A modification of the TCA cycle (glyoxylate cycle) lets plants and bacteria make carbohydrates from fats. to glucose isocitric lyase malate synthase Starch/glycogen synthesis glucose + ATP --> G-6-P + ADP hexokinase G-6-P --> G-1-P P-glucomutase UTP + G-1-P --> UDPG + PPi PPi + H2O --> 2 Pi U D PG pyrophosphorylase (plants: ATP, ADPG) PPP: Pentose Phosphate Pathway PPP: Pentose Phosphate Pathway P-pentose isomerase epimerase TK TA TK Using the PPP to make ribose Using the PPP to make NADPH Summary •Synthesis of glucose from pyruvate involves the glycolytic pathway plus 4 new enzymes. •Glycolysis and gluconeogenesis show reciprocal controls to prevent futile cycles. •Plants can direct acetyl-CoA to gluconeogenesis with the glyoxylate cycle. •Starch/glycogen synthesis involves UDPG (ADPG). •Pentose phosphate pathway provides a method for synthesizing NADPH and ribose, among other compounds.