Applications of Genetic
Engineering-Forensics!

Crime-Scene
Fact 1:
Whichever
suspect’s DNA
matches the
DNA found at
the crime scene
is our criminal!
Crime Scene
1
2
Applications of Genetic
Engineering-Forensics!




Key:
Lane “Crime
Scene” – Crime
scene sample cut
with Eco RI
Lane 1–Suspect 1
sample cut with
Eco RI
Lane 2–Suspect 2
sample cut with
Eco RI
Crime Scene
1
2
Applications of Genetic
Engineering-Forensics!




1. Which suspect
was at the crime
scene?
Suspect 2
2. How did you
determine that this
suspect was at the
crime scene?
The DNA bands
matched.
Crime Scene
1
2
Applications of Genetic
Engineering-Forensics!
DNA Fingerprinting Practice 2: Paternity
Case!
 The chart below shows the DNA of a mother,
a child and two potential fathers. All of the
child’s DNA must match up with the mother
and the father.

Applications of Genetic
Engineering-Forensics!
Lane 1:
Lane 2:
Lane 3:
Lane 4:
Mother’s DNA fragmentation pattern
Child’s DNA fragmentation pattern
Suspected father A’s fragmentation pattern
Suspected father B’s fragmentation pattern
1
bands
a
b
c
d
e
f
g
h
i
j
k
2
3
4
Applications of Genetic
Engineering-Forensics!
Lane 1:
Lane 2:
Lane 3:
Lane 4:
Mother’s DNA fragmentation pattern
Child’s DNA fragmentation pattern
Suspected father A’s fragmentation pattern
Suspected father B’s fragmentation pattern
1
bands
a
b
c
d
e
f
g
h
i
j
k
2
3
1. Which genes do the
mother and child
have in common?
4
Genes a, f, and h
Applications of Genetic
Engineering-Forensics!
Lane 1:
Lane 2:
Lane 3:
Lane 4:
Mother’s DNA fragmentation pattern
Child’s DNA fragmentation pattern
Suspected father A’s fragmentation pattern
Suspected father B’s fragmentation pattern
1
bands
a
b
c
d
e
f
g
h
i
j
k
2
3
2. Who is the father,
suspect A or
suspect B?
4
Suspect B
Applications of Genetic
Engineering-Forensics!
Lane 1:
Lane 2:
Lane 3:
Lane 4:
Mother’s DNA fragmentation pattern
Child’s DNA fragmentation pattern
Suspected father A’s fragmentation pattern
Suspected father B’s fragmentation pattern
1
2
3
4
3. How did you
determine who the
father of the child
was?
bands
a
b
c
d
e
f
g
h
i
j
k
The bands in the child
that did not match up
with the mother
matched up with
suspect B, which is
how I determined that
he was the father.
Paternity
Crime Scene