Biotechnology in Ukraine Ukraine Population: 42 million ppl Area: 603,628 km2 Ethnicity: 77.8% Ukrainians 17.3% Russians 4.9% others/unspecified Biotechnology of Plants Micropropagation of cloned plants: Meristem culture Callus tissue culture (proteins, polysaccharides, enzymes, etc.) Embryo culture Biotechnology of Plants Bacterial Desulphurization of Coals: using microorganisms of various taxonomic groups. Data about the reduction of sulphur content in the coals, mainly pyrite, under the action by representatives of mesophilic and moderately thermophilic acidophilic chemolithotrophic bacteria are given. Desulphurization of Coal Odessa Mechnikov University together with biotechnological center are involved in desulphurization of Ukrainian coal located in L’viv and Volyn’ coal mining regions, while combining the industry with precious metal mining, such as Germanium. Biotechnology of Plants Production of probiotics (Lactobacillus acidophilus, etc.) Production of medicinal plants Pharmacology: secondary metabolites ( humans use secondary metabolites as medicines, flavorings, and recreational drugs), antibiotics, monoclonal antibodies, starch, insulin Monoclonal antibodies Monoclonal antibodies (mAb or moAb) are antibodies that are made by identical immune cells that are all clones of a unique parent cell. Monoclonal antibodies can have monovalent affinity, in that they bind to the same epitope (the part of an antigen that is recognized by the antibody). In contrast, polyclonal antibodies bind to multiple epitopes and are usually made by several different plasma cell (antibody secreting immune cell) lineages. Bispecific monoclonal antibodies can also be engineered, by increasing the therapeutic targets of one single monoclonal antibody to two epitopes Biotechnology of Plants Propagation of endangered species Preservation of biodiversity Production and growth of virus-free plants November 1, 2017, IN VITRO PLANT, Research and Production Center, Odessa Ukraine Biotechnology of Plants Growth of plants selected for the genes of interest, disease resistance, elevated synthesis of the products of metabolism, herbicides resistance, stability of abiotic (expl. soil, water) and biotic factors GMO: Despite the new national legislation establishing biotech registries, due to the lack of required sublegislation only one biotech event (mentioned below) is legal for distribution in Ukraine for the purpose of animal feed. No other biotech events are currently legal for import, production, or sale in Ukraine (2016-11-10). Roundup Ready MON 40-3-2, in the form of soybean meal, is the only GE event in the official registry of approved feed sources that contain agricultural biotechnology for a term of five years from 2013- 2018. Alternative energy sources: production of bioethanol and electricity Corn is a major export item for Ukraine, and China is among its largest buyers of corn as the result of an agreement signed in 2013. The official protocol on phytosanitary and inspection requirements on corn export from Ukraine to China does not contain direct references for testing GE presence in shipments, however the protocol allows exports of products compliant with China’s phytosanitary legislation and standards. Technically such a framework might allow GE corn exports as China accepts GE-positive cargo, but only if the shipment is marked accordingly and contains only those GE events that are approved and allowed in China (see GAIN Report 14032 for further details) Biotechnology of Animals Animal breeding for the production of meat, lard, poultry and eggs Transgenic animals: rabbits, pigs, sheep, mice, rats Ukraine also approved the Antigen V-RG oral vaccine for carnivores “BrovaRabies V-RG” until October 2020, to be used for veterinary purposes. Aquaculture: fish, oysters (seaweed), crawfish, clams, shrimp Methods PCR ELISA CRISPR Cloning Cis-genes: do not contain foreign parts of genome, i.e. manipulation of genome with restriction enzymes Recombinant DNA technology References Microbiology and Biotechnology, N3 2017, Ukraine BACTERIAL DESULPHURIZATION OF COALS І. А. Блайда, Т. В. Васильєва (pp.6-23) "Micropropagation - Definitions from Dictionary.com". dictionary.reference.com. Retrieved 2008-03-17. "Frederick Campion Steward". Cornell University Faculty Memorial Statement. Archived from the original (PDF) on 2012-04-02. "Secondary metabolites - Knowledge Encyclopedia". www.biologyreference.com. Retrieved 2016-05-10. Ukraine Biotechnology Annual, USDA Foreign Agricultural Service report, 2016-11-10, UP1619