Supplementary figure 1. Representative negative lumogallion staining of grey and white matter of the frontal lobe of a 14-year-old male donor (A10), diagnosed with autism. Lumogallion staining found negative for the presence of aluminium produced a weak brown/yellow fluorescence emission in grey matter (a) with an elevated intensity typically noted in white matter areas (b). A weak green autofluorescence emission of the adjacent nonstained (5μm) serial sections (c & d) was observed with lipofuscin deposits producing a yellow fluorescence emission. Upper and lower panels depict magnified inserts of the fluorescence channel and bright field overlay. Magnification X 400, scale bars: 50μm.