MATERIALS Flat toothpicks Ice water Tape Stopwatch Goggles PROCEDURE: 1. Round 1: Obtain a pile of flat toothpicks 2. Without looking, and using only your thumb and index finger, break as many toothpicks as you can- one at a time. 3. Record your numbers while running a stopwatch Under table 1. Record how many toothpicks you broke at 10 seconds, then 30, 60, 120, and 180. 4. After the numbers have been recorded, set aside the broken toothpicks. 5. Round 2: Grab a pair of safety goggles and place them between your index finger and thumb. 6. Repeat steps 1-4 being sure to record these numbers under competitive inhibitor. 7. Round 3: Press your thumb against your fingers and have your lab partner tape your hand completely. Only a small bit of your fingertips should be exposed 8. Repeat steps 1-4 being sure to record these numbers under Denatured 9. Round 4: Soak your hand in ice water for as long as you can (up to 1.5 min). 10. Repeat steps 1-4 being sure to record these numbers under Ice 4. Did the rate of metabolized toothpicks decrease? If so, for which ones? 5. Explain the effect of the competitive inhibitor on the enzyme. 6. Explain how denaturation affects the function of an enzyme. 7. What happened to the metabolism of the toothpicks as the enzyme increased temperature in the ice water portion?