AP Bio Toothpickase

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MATERIALS
Flat toothpicks
Ice water
Tape
Stopwatch
Goggles
PROCEDURE:
1. Round 1: Obtain a pile of flat toothpicks
2. Without looking, and using only your thumb and index finger, break as many
toothpicks as you can- one at a time.
3. Record your numbers while running a stopwatch Under table 1. Record how many
toothpicks you broke at 10 seconds, then 30, 60, 120, and 180.
4. After the numbers have been recorded, set aside the broken toothpicks.
5. Round 2: Grab a pair of safety goggles and place them between your index finger
and thumb.
6. Repeat steps 1-4 being sure to record these numbers under competitive inhibitor.
7. Round 3: Press your thumb against your fingers and have your lab partner tape
your hand completely. Only a small bit of your fingertips should be exposed
8. Repeat steps 1-4 being sure to record these numbers under Denatured
9. Round 4: Soak your hand in ice water for as long as you can (up to 1.5 min).
10. Repeat steps 1-4 being sure to record these numbers under Ice
4. Did the rate of metabolized toothpicks decrease? If so, for which ones?
5. Explain the effect of the competitive inhibitor on the enzyme.
6. Explain how denaturation affects the function of an enzyme.
7. What happened to the metabolism of the toothpicks as the enzyme increased
temperature in the ice water portion?
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