OPERATOR’S MANUAL BIOPROtect II JR Vent to Room / Vent to C.E.C / Vent to H.E.C Bio-Containment Enclosure This manual includes information for installation, operation, maintenance and spare parts. We recommend that it be kept near the cabinet for ready reference. November, 2006 Rev C THE BAKER COMPANY 161 Gatehouse Rd., Sanford, Maine 04073 USA ⋅ (207) 324-8773 ⋅ 1-800-992-2537 ⋅ FAX (207) 324-2632 Internet address: http// www.bakerco.com “Creating Immaculate Atmospheres” http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc The Baker Company INTRODUCTION AND WELCOME It is a pleasure to welcome you to the growing number of customers who own and operate Baker biological safety cabinets. As the inventors of the laminar flow biological safety cabinet and the leaders in the field, Baker people take special pride in providing a cabinet that is designed for maximum performance. Your new BIOPROtect II JR cabinet includes many unique features which are included to give you superior performance, simpler maintenance and lower life cycle cost. Your BIOPROtect II JR cabinet is designed for both safety and value. In addition to the high quality you expect from all Baker equipment, this model has been ergonomically designed to provide the lab user with many exciting design features. The revolutionary ergonomic design will help prevent repetitive motion injury, reduce fatigue and lab accidents and enhance productivity. You will find your BIOPROtect II JR cabinet suitable for use not only for research and clinical diagnostic work involving tissue culturing of possibly infectious samples, but also for I.V. drug preparations and other pharmaceuticals that could have adverse health effects on operators and other techniques requiring a contamination-free atmosphere. Please note that all open-front containment cabinets, including this one, are for use with low to moderate risk agents only. Open-front cabinets do not provide absolute protection for the user. The adequacy of a cabinet for user safety should be determined on-site by an industrial hygienist, safety officer or other qualified person. Remember that you, the owner and user, are ultimately responsible and that you use your cabinet at your own risk. We recommend that this manual, along with factory test report, be kept near the cabinet for convenient reference by operators and qualified maintenance personnel. If you have any questions about the use or care of your new BIOPROtect II JR cabinet, please do not hesitate to contact our Customer Service Department at 800-992-2537 for assistance or e-mail us at bakerco@bakerco.com. Sincerely, Dennis Eagleson President, CEO The Baker Company, Inc. P.O. Drawer E, Sanford, Maine 04073 (207) 324-8773 1-800-992-2537 FAX (207) 324-3869 Visit our website at www.bakerco.com http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Table of Contents INTRODUCTION AND WELCOME ................................................................................................ i Table of Contents................................................................................................................................ ii I - FUNCTION AND DESCRIPTION OF THE BIOPROtect II JR CABINET .............................. 1 Airflow Inside the BIOPROtect II JR Cabinet ............................................................................... 1 Access to the Work Area ................................................................................................................ 2 Design Details ................................................................................................................................ 3 Performance assurance.............................................................................................................. 3 All-metal plenums..................................................................................................................... 3 Tested HEPA filters .................................................................................................................. 3 Work area lighting .................................................................................................................... 3 Iris Port...................................................................................................................................... 3 Optional Height Adjustable Cart .................................................................................................... 3 Specifications ................................................................................................................................. 4 Dimensions: .............................................................................................................................. 4 Weights ..................................................................................................................................... 4 Utilities: .......................................................................................................................................... 4 Mechanical ................................................................................................................................ 4 Electrical ................................................................................................................................... 4 Environmental Conditions.............................................................................................................. 5 Symbols and Terminology ............................................................................................................. 5 II - PREPARING THE BIOPROtect II JR CABINET FOR USE ..................................................... 6 The Uses of the BIOPROtect II Jr. ................................................................................................. 6 Location Within the Laboratory ..................................................................................................... 6 Operator Controls: .......................................................................................................................... 7 1.Blower On / Off Switch – ...................................................................................................... 7 2.Light On / Off Switch – ......................................................................................................... 7 3.Mute switch - ......................................................................................................................... 7 4 Left Side & Right Side Outlet On / Off –............................................................................... 7 Ground Fault Circuit Interrupter .................................................................................................... 8 Air Pressure Gage......................................................................... Error! Bookmark not defined. Air Pressure Alarm ......................................................................................................................... 8 Door Operations ............................................................................................................................. 9 To open the viewscreen: ........................................................................................................... 9 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc To open the door: ...................................................................................................................... 9 Removing the cart........................................................................................................................... 9 Set-up Procedure .......................................................................................................................... 10 Start-up procedure ........................................................................................................................ 11 Reacting to Spills.......................................................................................................................... 12 Decontamination........................................................................................................................... 13 About the HEPA Filters................................................................................................................ 15 IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES ............................................... 16 Filter Media and Seal Leak Tests ................................................................................................. 16 Procedure for filter leak tests........................................................................................................ 17 The Airflow Balance .................................................................................................................... 18 Airflow Smoke Pattern Test ......................................................................................................... 19 Grounding Continuity Test........................................................................................................... 19 Calibration procedure for Modus Airflow alarm(Digital Differential Pressure Monitor)............ 20 Initial Set Point Settings.......................................................................................................... 20 Low Flow Alarm Setting ........................................................................................................ 20 Check the Calibration ............................................................................................................. 20 Modus Programming Instructions ................................................................................................ 21 1.) Programming the Alarm Set points ................................................................................... 21 2.) Programming the Relay Dead band................................................................................... 21 3.) Returning to Normal Operation ......................................................................................... 21 Maintenance Notes ....................................................................................................................... 22 Replacing the HEPA Filter ........................................................................................................... 22 Changing the Exhaust Filter.................................................................................................... 22 Changing the Supply Filter ..................................................................................................... 23 V. - TROUBLE SHOOTING .......................................................................................................... 24 REPLACEMENT PARTS LIST .................................................................................................. 26 VI. APPENDIX ................................................................................................................................ 27 Modus Installation and Operation Handbook............................................................................... 28 BGA-BIOPROTECT II-JR Vent to Room Page 1 ....................................................................... 42 BGA-BIOPROTECT II-JR Vent to Room Page 2 ....................................................................... 43 BGA-BIOPROTECT II-JR Vent to Room Page 3 ....................................................................... 44 BGA-BIOPROTECT II-JR Canopy Exhaust Connection(CEC) Page 1 ..................................... 45 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc BGA-BIOPROTECT II-JR Canopy Exhaust Connection(CEC) Page 2 ..................................... 46 BGA-BIOPROTECT II-JR Canopy Exhaust Connection(CEC) Page 3 ..................................... 47 BGA-BIOPROTECT II-JR Hard Exhaust Connection(HEC) Page 1.......................................... 48 BGA-BIOPROTECT II-JR Hard Exhaust Connection(HEC) Page 2.......................................... 49 BGA-BIOPROTECT II-JR Hard Exhaust Connection(HEC) Page 3.......................................... 50 Wiring Diagram-Standard BIOPROtect JR.................................................................................. 51 Wiring Diagram – Master BIOPROtect JR .................................................................................. 53 WARRANTY ................................................................................................................................... 54 TEST REPORT…………………………………………………………………………ATTACHED http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc I - FUNCTION AND DESCRIPTION OF THE BIOPROtect II JR CABINET The BIOPROtect II JR is a Class II Bio-Containment Enclosure designed to house laboratory robotics or large apparatus that would not ordinarily fit in a standard Class II Biological Safety Cabinet (BSC). Some important features of the BIOPROtect II JR include, vertical laminar airflow, a front access opening, as well as supply & exhaust HEPA filters. The unit is designed to protect not only the environment and the people using the cabinet, but also the product within from airborne contaminants. The BIOPROtect II JR can be configured to vent three different ways. Vent to the room, Vent to a Canopy Exhaust Connection (C.E.C.) via a house exhaust system or, Vent to a Hard Exhaust Connection (H.E.C.) via a house exhaust system. Although your unit has passed a microbiological test, a modification of NSF/ANSI standard-49-2002e, the BIOPROtect II JR is not currently listed with the NSF. Airflow Inside the BIOPROtect II JR Cabinet Figure 1 shows the name and location of component parts and the typical airflow pattern of the BIOPROtect II JR The stainless steel metal diffuser (1) just below the supply HEPA (High Efficiency Particulate Air) filter assures that the work area is bathed in HEPA filtered air. The supply air stream splits so that some is directed into the front grille (2) and the balance is pulled into the perforated grilles on the rear wall (3). On a flat table top, in the absence of any obstacles, the air should split roughly in the center of the table. Because the cabinet must take in air to replace the air which is expelled through the exhaust filter, the same quantity of room air enters the cabinet through the eight inch front access opening. The flow of the intake air provides the personnel protection (containment) aspect of the cabinet. The front grille of the cabinet is integral to the large hinged door. This door, as well as the side and rear walls, are maintained under a negative pressure. Air is drawn from the front intake grille, into the hollow door, and into the right side wall. In this manner the outside air that is drawn into the cabinet as intake is not allowed to penetrate the table top area of the cabinet. Any and all contaminated air that is captured in the cabinet’s negative pressure plenums (front door, side walls, rear walls or upper plenum) is pulled into the cabinet’s blower (4). The blower discharges into a steel box that is a common plenum for the supply and exhaust HEPA filters (5). In this manner, all the air that is handled by the cabinet is HEPA filtered. The BIOPROtect II JR will exhaust a quantity of air (6) equal to that received at the front intake grille. If a leak should occur in a contaminated pressure plenum the negative pressure will create suction and pull air in, not allowing it to escape into the laboratory. If there is a leak from the positive pressure plenum, the surrounding negative pressure area will recapture the contaminated air and recirculate it through a HEPA filter. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 1 of 59 Figure 1 Typical section with airflow visualization arrows Access to the Work Area The BIOPROtect II JR is provided with three modes of access to the work area; via the large hinged door that can be opened to access the entire work area, the hinged viewscreen (providing access to the work surface) or from the 8” x 42” access (sash) opening between the bottom of the closed viewscreen and the air intake grate. The primary means of access must be through the 8” sash opening. This is the only means of access that will ensure the user protection from contaminants within the enclosure. The operator is only protected from airborne particulate when the large front door and the hinged viewscreen is closed and secured to provide an 8” x 42” access (sash) opening For easy set-up of equipment in the work area, the BIOPROtect II JR has one large door that exposes the entire work area from floor to ceiling to an area of 51.5” wide x 68” tall. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 2 of 59 Design Details Performance assurance Meticulous care in manufacturing is followed by more than 14 separate performance tests prior to shipment of your cabinet. In addition, a complete factory test report on the operation of your unit is included at the rear of this manual. All-metal plenums The work area surfaces and plenums of your BIOPROtect II JR have been constructed entirely of metal in order to provide strength, durability, air-tightness and resistance to deterioration. External construction is of 16-gauge cold-rolled steel, protected by a smooth white baked enamel finish. Tested HEPA filters Supply and exhaust HEPA filters in the BIOPROtect II JR unit have been found to be 99.99 percent effective on particles of 0.3 micron size. Each filter is scan-tested to assure leak-free installation. Work area lighting The fluorescent lights are externally mounted within the light canopy to minimize heat buildup in the work area. The unit uses two common F32T8 fluorescent bulbs powered by an efficient and reliable electronic ballast. Cable Port A 2.75” I.D. cable port is provided in each side wall to accommodate communications ports from process equipment without compromising performance. Optional Height Adjustable Cart The all stainless steel cart, which is optional on the BIOPROtect II JR was designed to accommodate process equipment weighing no more than 500 Lbs. It’s work surface dimensions are 44” Wide x 34” Deep. The height of the worksurface can be adjusted by the operator from 27” to 39” by using the manual crank provided at the front of the cart. For ideal ergonomic performance the cart should be adjusted so that the work table of the equipment is at the same level as the front intake grille of door on the front of the cabinet. The cart is provided with swivel casters, all of which are locking. To retract the cart from the work area, tip the foot operated locking devices at each front caster to a horizontal position then pull the cart out of the work area. To return the cart to the cabinet push the cart back into the work area and lock the front casters by pushing down on the foot operated locking devices. Make certain that the crank handle for the lift system is facing the front of the cabinet and is retracted under the work table before closing the door. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 3 of 59 Specifications Dimensions: Outside: 66” W x 64.44” D x 106.41”H Usable work area: 51”W x 36.5”D x 75”H (under diffuser) Cart worksurface: 44”W x 34” D x (27”- 37”) H (height adjusted by operator). Weights Unit weight: The weight of the BIOPROtect II JR cabinet is approximately 1200 lbs. Cart weight: The weight of the BIOPROtect II JR cart is approximately 250 lbs. Maximum worksurface capacity: 500 Lbs. uniform load. Point loads not to exceed 125 Lbs. each Utilities: Mechanical Four (4) 3/8” NPT female connections provided for plumbing; all are pre-plumbed to the top of the unit using 3/8” copper tubing. No service fixtures or valves are provided. User must supply fixtures to mate with 3/8” NPT female connection on side walls (see general arrangement drawing for details). Electrical !!! This cabinet is powered from more than one power source. The cabinet is not electrically safe unless all power sources are disconnected. Proper Lockout/Tag out procedures should be used !!! All electrical wiring should comply with the National Electric Code and any applicable Local Electrical Codes at the site of installation. The standard BIOPROtect II Jr has three junction boxes located on the front of the top deck. The following services need to be connected to the junction boxes. One 120Volt, 20Amp, 60 Hz, Single phase connection to power the blower. This circuit should be wired using 12 AWG wire and provided with a 20 Amp circuit breaker that will serve as a disconnect for this power supply. One 120 V, 15Amp, 60 Hz, Single phase connection for control power and power to the left side wall duplex. This circuit should be wired using 14 AWG wire and provided with a 15 Amp circuit breaker that will serve as a disconnect for this power supply. One 120 V, 15Amp, 60 Hz, Single phase connection for power to the right side wall duplex. This circuit should be wired using 14 AWG wire and provided with a 15 Amp circuit breaker that will serve as a disconnect for this power supply. The circuit breakers for these services are to be located in close proximity to the cabinet. Other connections may be required for optional equipment. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 4 of 59 The BIOPROtect II JR incorporates a solid-state ‘Stedivolt’ controller to adjust the blower motor speed. This unit compensates for some variation in incoming line voltage. The usable voltage range is 95 to 130V AC. You will need to consult the most recent certification test report on the unit to determine what the voltage setting should be for your unit. Environmental Conditions The BIOPROtect JR is designed to be used under the following conditions: Indoor use Altitudes up to 2000 meters Temperature range from 5° C to 40° C Relative humidity up to 80% Main supply voltage 105 to 125 VAC Maximum relative humidity 80 percent for temperatures up to 31°C decreasing linearly to 50 percent relative humidity at 40°C. Transient over voltages according to Installation Category(OVERVOLTAGE CATEGORIES) II per UL 61010A-1, Annex J. Pollution Degree 2 Symbols and Terminology Protective Earth: Any terminal which is intended for connection to an external protective conductor for protection against electric shock in case of a fault. ! General Caution: Refer to the instruction manual for information regarding personnel and environment protection.) http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 5 of 59 II - PREPARING THE BIOPROtect II JR CABINET FOR USE The Uses of the BIOPROtect II Jr. The BIOPROtect II JR cabinet has been designed to provide a work area that protects the experiment from the environment (Product protection), and protects the users from the experiment (personnel protection or ‘containment’). The laminar flow biological safety cabinet is designed for work with Biosafety Levels 1, 2 and 3 (low to moderate risk) agents as listed in The Center for Disease Control’s “Biosafety in Microbiological and Biomedical Laboratories”, U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and National Institutes of Health, U.S. Government Printing Office, Washington, D.C. 20402. HHS publication number (CDC) 84-8395. Extremely high risk agents assigned to Biosafety level 4 should never be used in this cabinet except in a certified BSL4 suit room. Please consult your safety professional for a proper risk assessment. Cautions * The use of any hazardous material in the cabinet requires that it be monitored by an industrial hygienist, safety officer or other qualified individual. * Explosive or flammable substances should never be used in the cabinet unless a qualified safety professional has evaluated the risk. * If hazardous biological work is to be performed, apply the appropriate biohazard decal which is enclosed. This is in accord with OSHA regulations, volume 39, number 125, Part II. * If chemical, radiological or other non-microbiological hazards are present, be sure to employ appropriate protective measures in addition to formaldehyde decontamination.Also see section 2 “Decon”. Call upon a suitably trained individual to monitor the operation. Location Within the Laboratory PROPER PLACEMENT WITHIN THE LABORATORY IS ESSENTIAL. The ideal location for any laminar flow biological safety cabinet is in a dead-end corner of the laboratory away from personnel traffic, vents, doors, windows or any other sources of disruptive air currents. Published research from The Baker Company (see Rake ASM paper, reference #34 in Appendix) and unpublished tests performed at the National Cancer Institute show that if a draft or other disruptive air current were to exceed the intake velocity of the cabinet, then contamination can enter the work area or escape from it. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 6 of 59 III - PROPER CABINET USE The BIOPROtect II Jr. is designed for continuous operation. The Baker Company recommends continuous operation to keep the interior work space clean and free of unwanted particles. If the blower is not running, the workspace of the cabinet will become contaminated with room air. If the cabinet’s use is not understood or the cabinet is operated incorrectly, it will not provide an adequate protective barrier. It would be helpful for operators to learn about the capabilities and limitations of the cabinet by reading some of the available literature. The Eagleson Institute offers several videotapes and an interactive CD which illustrate proper operation techniques for biological safety cabinets. A biological safety cabinet is a valuable supplement to good sterile technique, but is not a replacement for it. All activities that are to be performed in your cabinet should first be approved by a competent professional, such as an industrial hygienist or safety officer. This will ensure the cabinet is appropriate for the work it will be required to do. A competent professional should monitor the cabinet and its operating personnel at regular intervals to see that it is being used correctly. Operator Controls: The operator controls and indicators are arranged on the front panel of the unit. Blower On / Off Switch – This push button is a maintained action switch that controls the power to the cabinet blower. Pressing and releasing the push button from the UP/OFF position will change the switch to the DOWN/ON position. The cabinet blower will start. The GREEN indicator in the push button will illuminate. Pressing and releasing the push button from the DOWN/ON position will change the switch to the UP/OFF position. Caution: The cabinet audible alarm is disabled when the Blower switch is in the OFF position Light On / Off Switch – This pushbutton is a maintained action switch that controls the power to the cabinet fluorescent lights. Pressing and releasing the pushbutton from the UP/OFF position will change the switch to the DOWN/ON position. The cabinet lights will illuminate. The GREEN indicator in the pushbutton will illuminate. Pressing and releasing the pushbutton from the DOWN/ON position will change the switch to the UP/OFF position. Mute switch This pushbutton is a momentary action switch that temporarily mutes the audible alarm. A timer module inside the canopy controls the length of time that the alarm is muted. This is set at 5 minutes as a factory default but can be changed to 1 second to 100 hours. It may be desirable to increase this setting when cabinet decontamination is being performed. The YELLOW indicator in the pushbutton will illuminate while the alarm mute function is active. Should the alarm condition be remedied before the mute ‘times-out’ the timer will be reset and the mute indicator will not be illuminated. Left Side & Right Side Outlet On / Off – A separate 10 amp breaker switch is supplied for the remote switching of power to the right and left outlet receptacle located inside the BIOPROtect II JR. The breaker switches will ‘trip’ if the total electrical load on the circuit exceeds 10 amps. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 7 of 59 Ground Fault Circuit Interrupter Ground fault circuit interrupter (GFCI) outlets are installed on the 115V power circuit on this cabinet. The GFCI is designed to protect the operator from a possible electrical ground fault hazard. If a hazardous condition exists the GFCI will cut off electricity to the outlet. The button in the center of the unit will pop out. If the GFCI “trips” you should first determine and correct the cause of the problem, then press the button to reset the GFCI. Air Pressure Alarm The Air pressure alarm is controlled by a Modus Air Pressure indicator mounted in the control canopy. Operating & set up instructions for this instrument can be found in the On-Site Checks And Maintenance section of this manual. When either a High or Low flow condition is present for more than 3 seconds the audible alarm will be activated. Pressing the Mute switch will silence the alarm. The mute time is determined by the setting of a timer inside the control canopy. Factory setting of this timer is 5 minutes. Consult the test report for your cabinet for the factory set flow conditions for this alarm. The Baker Company recommends continuous operation to keep the interior work space clean and free of unwanted particles. Caution: The cabinet audible alarm is disabled when the Blower switch is in the OFF position This feature allows the user to turn-off the cabinet blowers when no longer required and the processes within the cabinet are found to be safe to personnel and the environment. Caution: It is recommended that prior to turning the Blower OFF you contact your health and safety officer for decontamination procedures.(also see section 2 “Decon”) http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 8 of 59 Door Operations During cabinet operation, the only access that should be permitted is through the 8” sash opening provided at the front of the cabinet. For set-up purposes, the viewscreen or the large front door can be opened to provide greater access. To open the viewscreen: Caution: Failure to latch the viewscreen in the open position may result in injury Grasp the acrylic panel and pull it away from the door to disengage the two grab catches on each side of the viewscreen. Swing the panel up to engage the support brackets on the cabinet’s top section. You must reach up and swing the two securing latches down to constrain the viewscreen in the open position. To open the door: With the viewscreen in the closed position, slide the dead bolt to the right to disengage the slide from the door jam. The deadbolt is located just inside the door and mates with a jam located on the left side wall. Pull the door open. Due to the cam rise action of the hinges the door will self close if left open. Swing the door 100 degrees (slightly more than perpendicular to the front of the cabinet) in order to override the self closing feature. Removing the cart Caution: It is recommended that prior to cart removal you contact your health and safety officer for decontamination procedures. (also see section 2 “Decon”) Should it be necessary to remove the cart, you must open the front door to fully expose the BIOPROtect II JR’s interior work bay. This area is assumed to have contamination so use caution in the way you approach the task. • Decontaminate and clean the unit as recommended by your health and safety officer. • Open the door to expose the cabinet’s work bay. • Any equipment on the cart should be disconnected from the side wall service connections and removed from the cable ports. • Unlock the front casters by pulling up the foot operated locking devices. When the locking device is in a horizontal position it is unlocked. • Use the hand crank to lower the height of the work surface if necessary. Check that your equipment will clear the front header if it is allowed to be left on the cart. • Pull the cart out and away from the BIOPROtect II JR. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 9 of 59 Set-up Procedure This section outlines the tasks required to properly install automated laboratory work stations within the BIOPROtect II JR. This does not replace the recommendations of the equipment manufacturer. It is assumed that the user is familiar with the set-up and operation of the ancillary equipment. • Place the equipment on the cart’s table top so that the equipment is within the area of the table top as defined by the four corners. The front of the equipment must face the front of the cart (crank handle side). • Using the crank handle provided on the cart, adjust the cart’s height until the work table of the equipment is flush or below the intake grill section of the door. Stow the crank handle under the work table. • Make utility connections to the side wall services. • Close the front door of the BIOPROtect II JR and make certain that you can perform any manual operations on the equipment through the 8” x 42” sash opening provided. • Make certain that no part of the equipment blocks or hangs over the front intake grilles. • Power and run all equipment. Make certain that the equipment and cabinet is operating properly and that none of the BIOPROtect II JR’s circuit breakers have ‘tripped’. • Have a certifier or safety officer witness the air currents (using a smoke tracer) to determine that the equipment does not compromise the cabinet’s ability to provide personnel and product protection. The user may choose to use a particle counter to evaluate the product protection capacity of the cabinet. A measurement of the personnel protection capacity of the cabinet (containment), should the user deem it necessary, will be a more involved test and will require a third party skilled in the operation of the BIO-analog test or the KI-discus test. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 10 of 59 Start-up procedure • If the unit has not been left running continuously, press the blower on/off switch. The switch will be illuminated when switched on. The cabinet’s audible alarm may activate for several seconds until the blower has come up to full speed. Make sure that you have cabinet airflow, either by listening for blower sound or feeling the airflow across your fingers. Check the reading on the air pressure gauge. The reading on the gauge should be consistent with the last time the unit was on. • Turn on the fluorescent light. The switch will become illuminated in the ‘on’ position and the fluorescent bulbs should activate. • Wipe down the interior area of the cabinet with a surface disinfectant. • • NOTES: • Some disinfectants may corrode or stain the steel surfaces. If this happens, clean the • surfaces afterward with a disinfectant-detergent and rinse with sterile tap water to prevent corrosion • Blocking the front and rear perforated grills must be avoided. If wipes or absorbent • towels are used on the work surface, be sure to keep them away from the grills. • Check that the position of the rear wall dampers (two upper and two lower) are consistent with the settings from the factory test report. • After your equipment is in place inside the cabinet, close and secure the door. All operations inside the BIOPROtect II JR must be carried out through the unit’s 8” sash opening. • Caution: The BIOPROtect II JR will not provide adequate personnel protection unless the front door and viewscreen are closed. • • After a procedure has been completed, all equipment which has been in contact with the research agent should be enclosed, and the entire surface decontaminated. Trays of discarded items should be covered. The cabinet should then be allowed to run for at least three minutes with no activity so that the airborne contaminant will be purged from the work area. • After you have removed all materials, culture apparatus, etc., decontamination of the interior surfaces should be repeated. Check the work area carefully for spilled or splashed nutrient which might support bacterial growth. And never use the cabinet to store supplies or laboratory equipment. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 11 of 59 Reacting to Spills Caution: An Emergency spill containment and clean-up procedure should be established prior to an accident Spilled fluid on the floor of the BIOPROtect II JR is contained within the stainless steel work area of the cabinet via the threshold placed under the unit’s door. The threshold will contain a volume of approximately 1 gallon (3 liters) of fluid. However, because the BIOPROtect II JR cabinet bears directly on the users floor there is no drain provided. • It is recommended that the researchers, in coordination with their consulting safety professional, have a written plan available in case of an accidental exposure or spill. • In the case of biological spill, for example, the area containing the spill may be flooded with an appropriate disinfectant. After the disinfectant has had time for a complete kill, remove the residue. If you have used a disinfectant which is harmful to stainless steel (Hypochlorite bleach solutions, for example) be sure that none remains to corrode cabinet surfaces. Clean the surfaces with sterile water. • The Center for Disease Control has published “Biosafety in Microbiological and Biomedical Laboratories.” If you have a spill involving a hazardous Biosafety Level 2 or 3 agent, then you are advised to leave the cabinet running so as to let the aerosols settle before you start cleanup procedures. With some spills, it may be necessary to decontaminate the room with an agent such as formaldehyde gas. (Biosafety Level 4 agents should never be used in the type of cabinet.) • If the spill contains volatile liquids which generate vapors creating a danger of fire or explosion, turn off the unit and other electrical equipment. Evacuate and seal the room and call for immediate help from a safety professional. • If the agent is a hazardous chemical, it may be recommended that a Spill Kit be kept readily available. This kit should be clearly labeled, and might include such items as a respirator, chemical splash goggles, two pairs of gloves, two sheets of absorbent material, spill control pillows, a solution to clean the contaminant, and waste disposal bags or other containers. Consult your safety professional for proper procedures and treatment of the specific agents you plan to use. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 12 of 59 Decontamination Whenever maintenance, service or repairs are needed in a contaminated area of your cabinet, the unit must first be decontaminated by an appropriate agent. The National Institute of Health, National Cancer Institute and the Center for Disease Control have all recommended the use of formaldehyde gas for most microbiological agents. Its application requires individuals who are experienced in the decontamination of cabinets, since the gas itself is toxic. Consult the NSF/ ANSI-standard-49-2002 Annex G. “Recommended Microbiological Decontamination Procedure”, contact NSF International, 789 North Dixboro Road, P.O. Box 130140, Ann Arbor Michigan 48113-0140. Whatever gas you choose, have the proper safety equipment (gas masks, protective clothing, etc.) within easy reach. In addition, you will want to be sure that the gas you are using will be effective against all of the biological agents within the cabinet. When you have decided which gas to use, post the antidote to it in a visible and nearby location. The volume of the BIOPROtect II JR cabinet is approximately 75 cubic feet, this will help you determine the required amount of decontaminating gas. Carcinogens present a unique chemical deactivation problem and the standard biological decontamination will not be effective against chemicals or other non-biologic materials. With materials of this kind, consult a qualified safety professional. Decontamination procedure Warning! This procedure should be performed by qualified technicians only. Surface-disinfect the inside of the window and all other surfaces on the view screen assembly. Multiply the total volume of the cabinet (75 ft3 ) by .3 gram/ft3 ) to determine the amount of paraformaldehyde required to decontaminate the cabinet. If the cabinet is vented to the outside you must consider the volume of the duct work in the paraformaldehyde calculation. Prior to sealing up the cabinet make sure all gas or flammable petcocks are closed and pressure tight. The inside cabinet work space must be at room temperature with 60% to 85% relative humidity. If relative humidity is low (below 60%) add boiling water in the work space. Monitor the inside of the cabinet with a humidity meter. Relatively humidity above 85% will require extra clean up which will be covered in step 15. Note: Without the proper relative humidity the formaldehyde gas will not be effective. The mode of entry of formaldehyde into the living organisms is through the cell wall by the absorption of water. Place a heating mantle with paraformaldehyde in the work space. The heating mantle must be able to reach 450 degrees F and must have a grounded plug which should be plugged in to an outlet outside the cabinet. Place a second heating mantle in the cabinet with 60% more by weight of ammonium bicarbonate and 10% more if using ammonium carbonate, than paraformaldehyde. This will be used later in step 13 to neutralize the formaldehyde gas. This step is recommended. Place spore strips inside the cabinet to confirm the decontamination process has been successful. If the cabinet is vented to the room, use a sheet of plastic and seal the front access and exhaust port openings. These openings should be sealed such that the exhaust airflow recirculates back to the front access opening. This will promote the even distribution of formaldehyde gas throughout the cabinet. If the cabinet is vented to the outside, then the exhaust transition should have a means to recirculate the exhaust airflow back to the cabinet blower if possible. This will promote the even distribution of formaldehyde gas through the exhaust filter. Seal the front access opening. Turn on the heating mantle containing the paraformaldehyde flakes. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 13 of 59 After 25% of the paraformaldehyde has depolymerized, turn on the cabinet blower for 10 to 15 seconds. Repeat after 50%, 75% and 100% of the paraformaldehyde has depolymerized, approx. 20 to 30 mins.. Allow the formaldehyde gas a minimum residence time of 6 hours, preferably over night. Turn on the heating mantle containing ammonium bicarbonate (NH4HCO3). After 25% of the ammonium bicarbonate has depolymerized turn on the cabinet blower for 10 to 15 seconds. Repeat after 50%, 75% and 100% of the chemical has depolymerized to neutralize the formaldehyde. Turn on the cabinet blower and allow the two gases to circulate for at least one hour. Check for formaldehyde by using a gas sampling device (dregger tube) to be sure the formaldehyde is at a safe level inside the lab space. Cut open the plastic and vent the cabinet to the outside. Aseptically remove spore strip and place in Trypticase-soy broth and incubate for 7 days. No growth will verify the decontamination process. When cleaning up, you may find residual paraformaldehyde (white powder) on the metal or glass surfaces. To remove this, use ammonia in warm water, wear gloves and wipe down the affected surfaces. The paraformaldehyde will dissolve in water and be neutralized by the ammonia. • http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 14 of 59 About the HEPA Filters The HEPA filter is one of the essential components of a biological safety cabinet. It is the shield which stands between the environment and the experimental agent. Some questions that the user should ask regarding the use of chemical agents in the BIOPROtect II JR are as follows: • Are these chemicals, either singly or in combination, able to attack filter components? Are these chemicals potentially toxic to the operator? Is there any combination of two or more which could be toxic? If the cabinet is being correctly used and only the operator’s hands and arms are inside the machine, then toxicity or irritation could occur through skin penetration. A proper evaluation of toxicity must deal not only with onetime exposure, but also with the effect of many small exposures over a period of time. • Are these chemicals explosive or flammable? If so, they should never be used in your cabinet. With a buildup caused by recirculation of air, an explosion can be the result of a motor spark or a burner operation in the work area. • In cases where chemical carcinogens, mutagens or teratogens are to be used, the risks should be carefully weighed in choosing a cabinet. Where the exhaust effluent contains a contaminant, it may need treatment. • The life of a filter is determined by how it is used and how often. Under normal laboratory conditions, you can expect up to five years of use. However, misuse or a heavy dust load within the cabinet will shorten any filter’s lifetime. Bunsen burners and misuse of chemicals will also shorten the useful life. • http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 15 of 59 IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES We recommend that the filter leak checks be performed before initial use, after relocation and after each filter change. They should also be carried out at regular intervals, usually six months or one year, as specified by an industrial hygienist, safety officer or other qualified person. As reported earlier in this manual, each individual cabinet made by The Baker Company is carefully tested before it leaves the factory. Your copy of the test report, which you will find at the back of this manual, gives the factory test results for your BIOPROtect II JR cabinet. Use it as your record of the original testing, and as your guide to testing in the future. To gain many years of satisfactory service; please be sure that your maintenance personnel come as close as possible to duplicating these original test figures. Your test procedures should be identical to ours so that comparing test results will have meaning. Please correspond directly with us to request detailed procedures for your particular cabinet model. Filter Media and Seal Leak Tests When preparing your cabinet for use after shipment, and then at prescribed intervals throughout its working life, you will need to verify that the filters have maintained their integrity. This is done by scan testing the filter faces and seals. The Institute of Environmental Sciences recommended practice IES-RPCC034.1 would be a good reference for this testing. The equipment needed will be: • An aerosol photometer. The instrument should sample air at a flow rate of 1 CFM. • A (D.O.P) generator with Laskin nozzle(s). Liquid dioctylphthalate (DOP), dioctylsebacate or comparable substance aerosolized by flowing air through the nozzle(s). When generated with Laskin type nozzle(s), the mean droplet size of the aerosol is 0.1 –3.0 micron with an average of 0.45 micron. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 16 of 59 Procedure for filter leak tests • Decontaminate the cabinet in accordance with NSF/ANSI standard-49-2002 and your health and safety officer. • Remove the front upper panel by first removing the ¼-20 hardware. Remove the damper from the front of the filter. This will expose the exhaust filter. • Find the 1/8” NPT hose barb on the top of the cabinet used for measuring the upstream concentration of challenge. • Turn on the aerosol photometer and set-up according to the manufacturer's instructions. • With the cabinet’s blower running, position the D.O.P. generator so as to introduce air generated smoke into any of the work bay openings (side wall to door openings or rear wall openings are all appropriate for introducing challenge). Measure the upstream concentration of the challenge. You must have a concentration of at lease 10 micrograms per liter. • Supply Filter: Remove the diffuser prior to scanning the filter. Holding the photometer probe no more than one inch from the filter face scan the entire surface area and perimeter (filter gasket frame area) in slightly overlapping strokes at a traverse rate of not more than ten feet per minute. • Exhaust filter: You may scan the downstream face of the exhaust filter from outside of the cabinet. • Eliminate leaks in the gasket frame area by re-tightening the filter gasket. • An unacceptable leak is defined as a reading greater than 0.01% http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 17 of 59 The Airflow Balance Warning! This procedure should be performed by qualified technicians only. The airflow balance which is set at the factory provides your unit with the air volume and velocity control to minimize leakage or airborne contamination either in or out of the work area. In order to duplicate as closely as possible the airflow characteristics described in the original factory test report, please follow these steps: 1. Make certain that the front door and viewscreen are closed and secured so as to make an 8” x 42” horizontal access opening. 2. Using a DIM (Direct Intake Measurement) measure the flow rate into the front access opening. Take at least five (5) consecutive readings. Average the readings and divide by 2.3 square feet (area of the front access opening). Acceptable range of the average shall be 100-110 FPM. 3. Using a hot wire anemometer mounted on a ring stand within the work area, take readings with the anemometer probe set 6” below the diffuser. The location of these readings should be the same as shown in the factory test report. The average of these velocity readings must be in the range between 50 and 70 FPM. 4. After you have compared your figures with those originally taken at the factory, make whatever adjustments are necessary. Use the motor speed control and the exhaust damper to bring the intake and downflow average values within the specifications. 5. As the HEPA filters load up with particulates, airflow will be maintained automatically to some extent. When the air flow is diminished you will have to increase the motor speed to maintain the specified intake and downflow velocity averages. This is done via the speed controller located in the light canopy. Warning! When the light canopy is lowered, some electrical parts are exposed. Do not perform this work unless you are a trained electrician or electronic technician. !!!!Note that terminals M1 & M2 as well as L1 & L2 are high Voltage.!!!! On the speed controller, turn the potentiometer clockwise until you have the desired airflow. Use a voltmeter to check the voltage across terminals M1 & M2. This is the voltage out to the motor. If the airflow cannot be maintained, it will necessary to replace the HEPA filters. (See “Procedure for HEPA Filter Replacement” later in this section.). http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 18 of 59 Airflow Smoke Pattern Test Airflow smoke patterns have been shown to vary depending on the equipment that is used inside the cabinet. The BIOPROtect II JR has been tested with the cart only. A description of this testing is summarized in the test report. However, when the BIOPROtect II JR is set-up properly (i.e. your set-up should not block or restrict the grilles and diffusers provided) the appearance of the airflow directly at the 8” access openings must be consistent with that described in the test report. To check for the direction of air movement, use a smoke generator and trace along the front access opening on the inside of the cabinet. Observe that no smoke is escaping from the work area. In order to be sure that room air is not entering the work area, trace along the outside of the front access opening. Observe that no smoke penetrates to the work surface. Grounding Continuity Test Using an Ohmmeter, set it to read the low resistance scale. Touch the two leads together and see that the display reads 0.1Ω to 0.0Ω. Touch one lead to the ground lug on the cabinet power cord while touching the other lead to bare metal on the unit where the user would be likely to touch the cabinet. According to NSF/ ANCI-standard 49-2202 the maximum allowable shall be 0.15Ω (Max allowable for UL is 0.50Ω ). http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 19 of 59 Calibration procedure for Modus Airflow alarm(Digital Differential Pressure Monitor). Note: This procedure MUST be followed every time the damper width has been changed. Initial Set Point Settings Balance the cabinet air flows to nominal operating set point per manufacturer instruction.( See instruction label on front of cabinet). Record voltage to motor/blower (M1, M2 on speed control) and measure and record damper/ slot width Make certain that the Modus displays 0.000 when zero pressure is applied(This can be easily accomplished by removing the Tygon tubing from the hose barb fitting). Use the “zero” function if necessary. Re-connect the tygon tubing and make sure that the value displayed is negative(-) when the cabinet is running. Low Flow Alarm Setting Place a DIM airflow hood on the front of the cabinet or use the 3 inch method for measuring inflow. Adjust cabinet to a 20% reduction in airflow by decreasing the voltage to motor. DO NOT adjust the damper Record the exhaust/ damper pressure on Modus (indication of exhaust plenum pressure at 20% reduction) and voltage to motor/ blower (across M1, M2 on speed control) Set the Modus High Alarm set point to the 20% reduction in pressure recorded in step 14 (per Modus programming instructions below) Set the Modus dead band to .002 (per Modus programming instructions below). Return cabinet to Nominal Set Point voltage, recorded in step 2 Check the Calibration Set Point Check While measuring the intake airflow with a DIM, reduce the airflow by decreasing the voltage to motor/ blower until a 20% reduction in flow is measured. Check that the low set point alarms at this value. If not, reset Modus low alarm set point to alarm as desired, per instructions. Return cabinet to Set Point Voltage. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 20 of 59 Modus Programming Instructions 1.) Programming the Alarm Set points After the first press of MODE, the HI LED flashes, indicating the HI set point is being entered. Press the ARROW key repeatedly to display the desired digit. Next, press the MODE to move towards the most significant digit, and repeatedly press the ARROW key to advance the digit’s value. Continue for the remaining digits. The sign of the set point (+ or -) is selected last. After the HI set point has been programmed, press the MODE to enter the LO set point. The LO alarm LED flashes, and you can repeat the above process to program the LO set point. 2.) Programming the Relay Dead band The dead band selection is provided to keep the relays from chattering when the pressure varies near the set point. To display the dead band, press MODE once after setting the LO set point polarity in the previous step. Both alarm LEDs flash. The three digits displayed are the dead band width, in the same units used for data display. Press the ARROW and MODE keys as described earlier to step the dead band to a value of .002. 3.) Returning to Normal Operation Press MODE once after the setting the dead band parameters. The digit “1” on the left indicates the unit is in analog setting mode. Since there are no analog outputs, the mode key can be pressed a final time to end programming and return to the normal pressure display. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 21 of 59 Maintenance Notes Replacing the HEPA Filter WARNING! Before any panels are removed, the cabinet must be decontaminated. The filters are sure to have collected microorganisms and other potentially harmful particles generated from the work area during their lifespan. Maintenance personnel should not allow themselves to be exposed. It should also be remembered that a specific gaseous decontaminant may work against microorganisms, but not against chemical agents. Where chemical agents are present, consult an industrial hygienist or other qualified person(s). If the differential pressure monitor presents an alarm, or if your periodic checks of total airflow show a drop of more than 5% of the nominal set point, the filters may be loading with particulates. As explained earlier in this section, the blower speed can be manually increased to compensate for filter loading. However, when the airflow can no longer be maintained or when the filters are damaged, they need to be replaced. A contaminated filter must be handled with caution. Personnel should be protected by clothing or breathing apparatus as necessary to the nature of the hazard. It is advisable to seal the contaminated side of the filter by taping a plastic sheet or cardboard over the face before removal. This should minimize the number of particles shaken loose from the filter. Once removed, the filter should immediately be sealed in a biological hazard bag and then disposed of safely in accordance with environmental regulations. After filter replacement has been completed, the cabinet and the room should be cleaned and decontaminated in a manner consistent with the nature of the hazardous material. The cleaning materials, along with the protective gear and clothing, should be properly disposed of. HEPA filters are very easily damaged, and you will want to use great care during handling so as to avoid injury to the filter media and gasket surface. When installing the new filters, it is a good idea to tape a piece of cardboard over the filter media to give protection against dropped wrenches or misdirected fingers. Of course, you will need to make sure that the cardboard is removed before the access panels are re-installed. Inspect the filters carefully before and after installation. A broken or damaged filter is worthless. Changing the Exhaust Filter The BIOPROtect II JR requires one exhaust filter measuring 19” x 38” x 12” deep (consult test report for part numbers and specifications). This filter is arranged to face the front of the unit so as to eliminate the nuisance of ceiling restrictions on the exhaust air. To service the exhaust filter remove the panel from the front of the unit using a 3/8” nut driver or socket to remove the ¼-20 hardware. Using the same 3/8” driver, remove the hardware from the filter securing frame. Pull the frame and filter away from the unit. Prepare the new filter by putting a light coat of silicone grease on the face of the gasket. Clean the sealing flange thoroughly. Then carefully slide the new filter into place and make sure that it is properly seated on the flange. Replace the filter clamp assembly and perform the leak test described earlier in this chapter. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 22 of 59 Changing the Supply Filter The BIOPROtect II JR requires one supply filter measuring 32” x 45” x 3” deep (consult test report for part numbers and specifications). This filter is arranged so as to be serviced from inside the BIOPROtect II JR’s work area. Consequently you will have to remove the cart and any equipment from inside the cabinet prior to changing the filters. To service the supply filter first swing the diffuser down allowing it to rest on the rear wall by removing the ¼-20 screws at the front edge of the diffuser panel. Next using a 3/8” driver remove the ¼20 hardware that secures the frame to the filter. This frame supports the filter. Prepare the new filter by putting a light coat of silicone grease on the face of the gasket. Clean the sealing flange thoroughly. Place the new filter in the center of the frame and install the frame into the cabinet using the ¼-20 hardware. Conduct a filter leak check as described in the earlier chapter. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 23 of 59 V. - TROUBLE SHOOTING Here are some suggestions based on our experience with the use and misuse of biological safety cabinets. CAUTION: Whenever the potentially contaminated areas of your cabinet must be entered, make sure that the unit is first decontaminated by use of an appropriate method by qualified personnel. When a smoke test indicates that there is air flowing from the interior of your cabinet into the surrounding room: • Make certain that the front door is closed and the front intake grilles are not blocked. This will create an 8” x 42” horizontal opening in the front of the cabinet. • If your cabinet is connected to an in-house exhaust, make sure that there is adequate exhaust suction and no back pressure. Also, be sure the dampers are open. Re-balance the exhaust system to handle an adequate quantity of air and static pressure (suction). Consult with building maintenance people. • The exhaust filter may be loaded with dirt if the unit has been in service for some time. Decontaminate, and replace the HEPA filter. • There may be high cross-drafts in the room which are causing the outflow of smoke. Check the airflow balance, following the procedure recommended in an earlier section of this chapter. Eliminate the source of the cross-draft. If there is no airflow within the work area: • Make sure that the blower switch is turned on. The indicator on the pushbutton should be illuminated. • If the lights are working, then turn the blower switch off and let the cabinet rest for ten minutes. When the time has passed, turn the blower switch on again. If the blower starts, you know there has been overheating of the blower motor. Also check the wiring connections inside of the light canopy which connects the canopy to the cabinet. Be sure the connections are pushed together. • If these solutions do not correct the problem, or if the blower failed to start after the rest period, then either the speed control, blower motor or capacitor is defective. A qualified electrician can find out if the speed control is defective by bypassing it, using the wiring diagram in the Appendix of this manual as a guide. If there is a noise problem, it may be caused by motor bearings. When there is uneven fan operation, or noise from the motor/blower assembly: • • It may be necessary to decontaminate the cabinet. • Look for loose objects in the fan cage. • Check to see if the fan wheel is contacting the blower housing. Open the light canopy and check the multi-pin connectors at each end to be sure they are securely engaged. • Another possible source of the problem is the speed control. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 24 of 59 If the fluorescent light does not work • Make sure that the light switch is turned on. The indicator on the pushbutton should be illuminated. • Make certain that the bulbs are installed properly into the clips. • Open the light canopy and check the multi-pin connectors at each end to be sure they are securely engaged. If the lamp flickers, there is probably an improper ground. The wiring can be traced to the source of a break. http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 25 of 59 REPLACEMENT PARTS LIST PART NAME QTY BAKER COMPANY PART NUMBER Blower 1 11432 Capacitor 1 11558 Motor (120 V, ½ HP, 1620 RPM) 1 31679 Filter, exhaust, 19” x 38” x 12” 1 41175 Filter, supply, 32” x 45” x 3” 1 38913 Power Supply, 24V DC, 1A 1 39324 Motor speed control 1 39658 Fluorescent lamp 2 17927 Ballast, fluorescent 1 34160 Pressure Monitor/Alarm 1 38996 Hydraulic pump kit (cart) 1 40555 NOTE: When ordering replacement parts, please furnish serial number and model number of unit http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 26 of 59 VI. APPENDIX http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 27 of 59 Modus Installation and Operation Handbook http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 28 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 29 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 30 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 31 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 32 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 33 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 34 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 35 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 36 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 37 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 38 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 39 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 40 of 59 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 41 of 59 BGA-BIOPROTECT II-JR Vent to Room Page 1 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 42 of 59 BGA-BIOPROTECT II-JR Vent to Room Page 2 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 43 of 59 BGA-BIOPROTECT II-JR Vent to Room Page 3 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 44 of 59 BGA-BIOPROTECT II-JR Canopy Exhaust Connection(CEC) Page 1 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 45 of 59 BGA-BIOPROTECT II-JR Canopy Exhaust Connection(CEC) Page 2 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 46 of 59 BGA-BIOPROTECT II-JR Canopy Exhaust Connection(CEC) Page 3 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 47 of 59 BGA-BIOPROTECT II-JR Hard Exhaust Connection(HEC) Page 1 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 48 of 59 BGA-BIOPROTECT II-JR Hard Exhaust Connection(HEC) Page 2 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 49 of 59 BGA-BIOPROTECT II-JR Hard Exhaust Connection(HEC) Page 3 http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 50 of 59 BIOPROTECT II-JR OPTIONAL CART http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 51 of 59 Wiring Diagram-Standard BIOPROtect JR http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 52 of 59 Wiring Diagram – Master BIOPROtect JR http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 53 of 59 WARRANTY The Baker Company, Inc. expressly represents and warrants all goods (a) to be as specified in the Baker Company catalogs and literature, and (b) to be free under normal use, service and testing (all as described in the Baker Company catalogs and literature) from defects in material and workmanship for a period of twelve months from the invoice date. The exclusive remedy for any breach or violation of this warranty is as follows: The Baker Company, Inc. will, F.O.B. Sanford, Maine furnish without charge repairs to or replacement of the parts or equipment which proved defective in material or workmanship. No claim may be made for any incidental or consequential damages. This warranty is expressly in lieu of all other warranties, express or implied, including any implied warranty of merchantability or fitness for a particular purpose, unless otherwise agreed in writing signed by the Baker Company. (The Baker Company shall not be responsible for any improper use, installation, service, or testing of the goods). http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc Page 54 of 59