International Journal of Pharmaceutical Science and Health Care
Available online on http://www.rspublication.com/ijphc/index.html
Issue 6, Vol. 3 (May-June 2016)
ISSN 2249 – 5738
INVITRO ANTI MCIROBIAL ACTIVITY STUDY OF CRUDE EXTRACT
FROM SPHAGNETICOLA TRILOBATA
Devika R, Krishna Priya S, Padmapriya M and Sugashini PK
Department of Biotechnology, Aarupadai Veedu Institute Of Technology,
Paiyanoor.
ABSTRACT :
Since ancient times , use of plants as a source of medicine has been the inherent part of
life in India. There are more than 3000 officially documented plants in India that holds great
medicinal potential in modern medicine and in traditional systems. Therapeutic properties of
medicinal plants are useful in curing various diseases, advantageous and 100% natural.
Sphagneticola trilobata commonly known as Singapore Daisy. Wedelia and Creeping Oxeye is
found wide spread as mat – forming perennial herb. In the present investigation, the crude extract
of leaf and flower were subjected to anti microbial studies at various concentrations (30µL,
60µL, 90µL and 120µL) and the results were represented in terms of zone of inhibition. A
significant
results were recorded and the crude extract showed resistant to the targeted
organisms proved that the extracts have high therapeutic value.
Keywords : Antimicrobial , therapeutic, phytochemicals, inhibition , sensitive, resistant.
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International Journal of Pharmaceutical Science and Health Care
Available online on http://www.rspublication.com/ijphc/index.html
Issue 6, Vol. 3 (May-June 2016)
ISSN 2249 – 5738
INTRODUCTION :
For several years, natural plant phytochemicals are traditionally used as medicines and
about 10% of flowering plants on Earth has been proved to treat various infections and gained
importance among the pharmaceutical industries [1,2]. The phytochemicals possess antioxidant
[3], antinociceptive [4], anti – inflammatory [5,6], antimicrobial [7], antihelminthic [8],
hepatoprotective [9] , antiproliferative [10]
and antifungal activities [11].The therapeutic
importance of a plant may be due to the presence of bioactive compounds such as flavanoids,
glycoside, resins, phenols, tannins, alkaloids, saponins, steroids, triterpenoids etc. [12].
Plant polyphones are known to have high radical scavenging activity and also effective
antiulcer, anti-inflammatory and reduces the side effects like neurodegenerative diseases,
Cardiovascular liver cirrhosis and hepatitis [13,14]. The methanolic fruit extract of Benincasa
hispida are traditionally used to treat jaundice, dyspepsia , fever, menstrual disorders and renal
diseases [15], antihistaminic acid [16] and anti depressant activities [17]. In the present
investigation, the leaf and flower extracts of Sphagneticola trilobata were subjected to
antimicrobial activity study with few Gram positive and Gram negative bacteria.
MATERIALS AND METHODS
Antimicrobial Study
Microbial culture






Escherichia coli
Klebsiella pneumonia
Protease mirabilis
Pseudomonas aeruginosa
Salmonella typhi
Streptococcus aureus
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International Journal of Pharmaceutical Science and Health Care
Available online on http://www.rspublication.com/ijphc/index.html
Issue 6, Vol. 3 (May-June 2016)
ISSN 2249 – 5738
PROCEDURE
A pure culture of the above target organisms were obtained from MTCC, INTECH ,
Chandigarh and were culture in nutrient broth for antibacterial study in the present study.
Antibacterial activity study of all the organisms were conducted in nutrient broth for antibacterial
study in the present study. Antibacterial activity study of all the organisms were conducted with
Muller and Hinter Agar by Kirby-Bauer Disk Diffusion method. The test organisms were
swabbed onto the duplicate petriplates and five wells were made with the help of sterile cork
borer. Control extracts of leaf and flower (30µL, 60µL, 90µL and 120µL) were pipette out
aseptically in the wells and incubated for 24 hours and the zone of inhibition were measured as
per standard methods.
RESULTS AND DISCUSSION
Sphagneticola trilobata plant is found to be wide spread and the extracts of leaf and
flower registered high zone of inhibition when it was subjected to antimicrobial activity study
and the results are tabulated in Table 1.The extracts were pipette out in sterile condition in their
respective wells with a negative control in the middle. The leaf extract recorded the maximum
inhibitory zone with Escherichia coli and Pseudomonas aeruginosa at 120 µL with 1.4 mm
diameter zone of inhibition followed by 1.3 mm with Staphylococcus aureus and Klebsiella
pneumonia. The third zone of inhibition was registered by Salmonella typhi and Protease
mirabilis during the period of study. The root extract of B..tinctoria exhibited significant
antibacterial activity against Pseudomonas aeruginosa and Escherichia coli [18]. The methaolic
extract of Trichodesma indicum (Linn) R. Br. showed maximum zone of inhibition (10% more)
than the standard Neosporin against Escherichia coli [19] and the extract had good antioxidant
[20,21] activity against nitric oxide radical generation.
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International Journal of Pharmaceutical Science and Health Care
Available online on http://www.rspublication.com/ijphc/index.html
Issue 6, Vol. 3 (May-June 2016)
ISSN 2249 – 5738
The flower extract of Sphagneticola trilobata was found to be relatively sensitive to all
target bacterial organisms. The maximum zone of inhibition (1.4mm) was observed with
Escherichia coli, Pseudomonas aeruginosa , Klebsiella pneumonia
and Protease mirabilis
followed by Staphylococcus aureus (1.3mm) and Salmonella typhi . The ethanolic extracts of
Acacia nilotica, Syzygium aromaticum and Cinnamum zeylanicum showed the most potent
activity against all the micro organisms (E. faecalis, Staphylococcus aureus, S. bovis and S.
mutans) [ 22]. A. nilotica is hardly susceptible to the gram negative bacteria in doses less than
2*105µg/ mL [23]. The antimicrobial potency of medicinal plants are due to the bioactive
compounds such as tannins, phenols, saponins, flavanoids etc. and they show good active against
multidrug resistant strains [24].
TABLE 1: Antimicrobial studies of S. trilobata
ORGANISM
ZONE OF INHIBITION(mm)
30
LEAF
FLOWER
CONCENTRATION (µL)
60
90
120 30
60
90
120
Escherichia coli
0.9
1.0
1.2
1.4
0.9
1.0
1.2
1.4
Pseudomonas aeruginosa
0.9
1.0
1.1
1.4
0.9
1.1
1.2
1.4
Staphylococcus aureus
0.9
1.0
1.2
1.3
0.9
1.0
1.1
1.3
Klebsiella pneumonia
0.9
1.0
1.2
1.3
0.9
1.2
1.3
1.4
Salmonella typhi
0.9
1.0
1.1
1.2
0.9
1.0
1.1
1.3
Protease mirabilis
0.9
1.0
1.1
1.2
0.9
1.0
1.2
1.4
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International Journal of Pharmaceutical Science and Health Care
Available online on http://www.rspublication.com/ijphc/index.html
Issue 6, Vol. 3 (May-June 2016)
ISSN 2249 – 5738
CONCLUSION:
In the present investigation, Sphagneticola trilobata showed sensitive result over the
targeted organisms and proved that the phytochemicals are active against the strains and on
further investigation with particular phytochemical will provide a clear picture of their activity
with distinct therapeutic value.
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International Journal of Pharmaceutical Science and Health Care
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International Journal of Pharmaceutical Science and Health Care
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International Journal of Pharmaceutical Science and Health Care
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Issue 6, Vol. 3 (May-June 2016)
ISSN 2249 – 5738
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