JOURNAL OF THE DRYLANDS 4(2): 333-340, 2011 Effect of Selected Faba Bean (Vicia faba L.) Varietal Difference on Straw DM Yield, Chemical Composition and Nutritional Quality Yetimwork Gebremeskel1, Awet Estifanos2* and Solomon Melaku3 Yetimwork Gebremeskel, Awet Estifanos and Solomon Melaku. 2011. Effect of Selected Faba Bean (Vicia faba L.) Varietal Difference on Straw DM Yield, Chemical Composition and Nutritional Quality. Journal of the Drylands 4(2): 333-340. The study was conducted at Haramaya University Campus and Hirna Experimental Stations, Ethiopia, during the 2004 cropping season with the objective of determining leaf to stem ratio (L:S), straw dry matter yield (SDMY), harvest index (HI), potential utility index (PUI), chemical composition, in vitro dry Matter Yield (IVDMY) and in sacco dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) degradability. The experiment was conducted using five selected varieties of faba bean in Randomized Complete Block design (RCBD) with three replications. The overall result showed that varietal differences in grain yield, SDMY and straw quality and this indicated that the possibility of selecting for faba bean varieties that combine high grain yield and desirable straw characteristic. Based on the present findings it can be concluded that Tesfa, Mesay and local bean were identified to have high grain yield and better nutritive value than the others. Key words: Variety, Straw Dry matter Yield, chemical composition and nutritional quality 1 Debrebirhan University, P.O. Box 445, Debrebirhan, Amhara, Ethiopia, yetger@yahoo.com 2 Tigray Agricultural Research Institute, P.O.Box 492, Mekelle, Tigray, Ethiopia, email- aweyetir@yahoo.com 3 Late, Haramaya University, P.O. Box 138, Dire Dawa, Ethiopia *Author of correspondence: Awet Estifanos, tel-+251344419607, Fax-+251344408028, P.O.Box-492, emailaweyetir@yahoo.com Received: August 10, 2011, Accepted, November, 2011 INTRODUCTION In Ethiopia the majority of cattle population (70%) is reared in the highlands settled crop farming area, where animal feed production is a serious problem (Alemayehu, 2004). It is obvious that the expansion in the cultivation of food crops increase the supply for crop residues for animal feeding and different crop residues contribute 50% of the total animal feed requirement in the crop-livestock farming area (Jutzi et al 1987). Many Ethiopian farmers conserve crop residues for use during the critical period of the dry season. Among the pulses, faba bean (Vicia faba L.) covers about 280,190 to 380,570 hectares of land, which accounts for about 40-48% of the total area cultivated by pulses. Ethiopia is the second largest producer of faba bean in the world next to China (Asfaw et al1993). Currently, according to the National Agricultural Input Authority (2003) a total area of 369,000 ha is covered by faba bean from which 447 thousand tons of grain are produced. Although, faba bean is grown primarily for human food, it can also be made into silage, as animal feed. In addition to being used as animal feed, the straw is used for brick making and as a fuel in some parts of Sudan and Ethiopian (Bond et al 1985). According to Kossila (1984), a straw of faba bean is rich in protein, calcium and magnesium than cereal straws, and if properly harvested, it is useful roughage feeds for ruminant animals. Generally, pulse straws contain 10-15% crude protein (CP) in DM and their Copyright © Journal of the Drylands 2011 ISSN 1817-3322 energy content is higher compared to the respective cereals by-products and sugar cane, with satisfactory palatability (Kossila 1984). This experiment was thus conducted to evaluate the varietal differences in dry matter yield, chemical composition, in vitro dry matter digestibility and in sacco degradability of selected varieties of faba bean straw. MATERIALS AND METHODS The Study Area The study was conducted at Hirna and Haramaya University campus Experiment Station on Vertisols. Hirna is located at 41o4’ E longitude and 9o12’ N latitude, and at an altitude of 1870 m.a.s.l. It has an annual rainfall of 990-1010 mm, with a mean temperature of 24oC. Haramaya University is located at 42o3’ E longitude and 9o26’ N latitude and at an altitude of 1980 m.a.s.l. The mean annual rainfall at Haramaya is 780 mm and the mean annual temperature is 23.40C (AUA, 1998). Experimental Design and Treatments The experimental treatments consisted of five varieties of faba bean that were selected on the bases of their adaptability to the agro-ecological zones of the two sites from earlier screening trial. The varieties used in the study were Tesfa, Bulga70, CS-20-DK, Mesay, and local bean. The land was prepared at the two sites according to the acceptable cultural practices for 333 faba bean. Ploughing was done during the short rainy season in March, followed by once harrowing using disc harrow in early June and a slight hoeing was made to loosen the soil. The land was then classified into plots as per the design of the experiment. Each variety of faba bean was sown on 4 m by 3.2 m plot area with 0.8 m between plots. The spacing between rows and plants within row were 0.4 m by 0.1m, respectively. Data Collection Close observation was made after sowing to assess the rate of germination and early establishment performance of the varieties. In order to support the visual assessment of the establishment performance of the varieties, seedling counts were made on the whole plot on the 23rd day of sowing. The experimental plots were weeded three weeks after sowing and thereafter as required based on the occurrence of weeds. Leaf : stem ratio was calculated by taking ten randomly selected plants from each plot and separating them into leaf and stem fraction manually, and weighing each fraction separately in order to take their relative proportion on DM basis. Straw Dry Matter Yield, Grain Yield, Potential Utility Index and Harvest Index At maturity on average of three months, all varieties were harvested to asses straw DM yield. The straw dry matter yield (SDMY) was calculated according to the formula developed by Tarawali et al (1995). Where: TFW= Total fresh weight, DM%=Dry matter percentage of the straws. Grain yield was calculated as the total weight of seeds harvested per plot. Potential utility index (PUI) was calculated as the ratio of grain yield plus digestible DM yield of crop residue to total above ground biomass DM yield (Fleischer et al 1989). The in vitro dry matter digestibilityI (IVDMD) was used for calculation of the digestible crop residue DM yield Harvest index (HI) was calculated as the percent proportion of grain yield to total above ground DM yield (Fleischer et al 1989). In vitro Dry Matter Digestibility Procedure In vitro dry matter digestibility of the straw sample of each variety was determined by Tilley and Terry method as modified by Van Soest and Robertson (1985). In sacco Degradability Procedure The dried faba bean straw samples were ground to pass through a 2 mm screen to determine DM, OM and NDF degradability. Rumen degradability of the samples were determined by incubating 2.5 g of sample of faba bean straw in a nylon bag (41 μm pore size and 6.5 x 14 cm dimension) in three rumen fistulated Boran x Holestein Fresian steers kept at the International Livestock Research Institute (ILRI), Debre Zeit Research Station. The straw samples were incubated for 0, 3, 6, 12, 24, 48, 72, 96 and 120 hours. The DM, OM and NDF degradability data were fitted to the experimental equation described by McDonald (1981); Where: Y= DM degradability at time (t); a= rapidly degradable fraction; b= potentially degradable fraction, but slowly degradable fraction; c= rate of degradation of b; tl= lag time Chemical Analysis The dried samples of each variety of faba bean straws were ground to pass through a 1 mm screen to determine their chemical composition. Dry matter percentages of the different samples were determined by oven drying the samples at 105 0C for 24 hours. Total ash and CP content were determined according to the procedure of AOAC (1990). Neutral detergent fibers (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL) were analyzed using the detergent extraction method (Van Soest et al., 1991). Hemicellulose was calculated by subtracting ADF from NDF and cellulose fraction was calculated as the difference between ADF and ADL. Statistical Analysis Data on agronomic parameters and straw dry matter yield were subjected to analysis of variance (ANOVA) using the statistical software MSTAT-C (1989). Chemical composition, IVDMD, in sacco DM degradability (DMD), OM degradability (OMD) and NDF degradability (NDFD) data were analyzed using the General Linear Model (GLM) procedure of the statistical analysis system (SAS 1998). Treatment means were separated by least significance difference (LSD). 334 The model used for analysis of the nylon bag data was; Where, Yijk = response variable; μ = overall mean ai = animal effect pj = incubation time rk = sample effect eijk= random error For chemical composition, agronomic parameters and IVDMD the model used was; Where, Yij = response variable μ = overall mean Ti = treatment effect βj = Block effect eij = the random error RESULTS Leaf to Stem Ratio, Grain Yield, Harvest Index and Potential Utility Index Leaf to stem ratio (LSR) had no significant difference (P>0.05) among the faba bean variances both at Haramaya and Hirna sites (table 1). There were significant yield difference (P<0.05) among the faba bean varieties both at Haramaya and Hirna. Mesay and local bean varieties produced significantly higher (P<0.05) grain yield than Bulga-70 ad CS-20-DK at Haramaya where as Tesfa and Mesay yielded significantly more (P<0.05) grain than CS-20-DK and local bean at Hirna. Harvest index was significantly higher (P<0.05) for Mesay compared to the other faba bean varieties at Haramaya whereas, there was no significant difference among the varieties at Hirna. There was significant difference (P<0.001) in PUI among the faba bean both at Haramaya and Hirna. The value of PUI ranged from 60.7 to 79.5 and 41.3 to 50.6 at Haramaya and Hirna, respectively Straw Dry Matter Yield, Straw to Grain Ratio and In vitro Dry Matter Digestibility The faba bean varieties were significantly different (P<0.001) in SDMY at Haramaya only. Local bean produced significantly higher (P<0.05) SDMY than the rest varieties. However, the varieties were significantly different in the value of straw to grain ratio both at Haramaya and hirna with the mean value of 1.56 and 3.22. The faba bean varieties were significantly different (P<0.05) in IVDMD value at Hirna where as there was no significant difference at Haramaya. At Hirna the variety Bulga-70 was relatively higher in IVDMD value than the rest varieties. Chemical Composition Both at Haramaya and Hirna there was no significant difference (P>0.05) among the faba bean varieties for dry matter, ash, organic matter and crude protein contents. The CP result in this study was greater than the value of 7.2%, which is reported by Luleseged and Jemal (1989). Table 1. Mean leaf to stem ratio, grain yield, harvest index and potential utility index of faba bean varieties at Haramaya and Hirna. Leaf to stem ratio Grain yield (t/ha) Harvest index (%) PUI Varieties HAU HI HAU HI HAU HI HAU HI Tesfa 0.66 0.28 3.63ab 2.54a 42.4b 27.4 71.6b 43.5b bc ab b a Bulga-70 0.82 0.30 3.25 2.46 39.9 26.8 79.5 44.3b c c b c CS-20-DK 0.68 0.39 3.10 1.97 39.2 26.9 60.7 50.6a a a a b Mesay 0.55 0.30 3.98 2.62 49.6 27.56 71.4 44.9b a bc b c Local bean 0.66 0.27 3.91 2.08 41.9 23.9 62.2 41.3b Significant level ns ns * * * ns *** *** Overall mean ± SEM 0674 0.308 ± 3.574 ± 2.334 ± 42.6 26.51± 69.08 44.92 ± 0.06 0.02 0.06 0.10 ± 0.99 0.85 ± 1.09 ± 0.83 C.V (%) 19.97 11.84 0.02 9.61 4.65 7.20 2.73 3.24 a, b, c Means with different superscript in the same column are significantly different; SEM= standard error of mean; CV= coefficient of variation; *P<0.05; **P<0.01; ns= non significant; HAU= Haramaya University; HI= Hirna. Neutral Detergent Fiber, Acid Detergent Fiber and Acid Detergent Lignin There were significant differences among the faba bean varieties for the plant cell wall content (Table 4). The NDF content ranged between 33.1% (CS20-DK) to 40.9% (Tesfa) at Haramaya and 39.6% (Tesfa) to 51.5% (Mesay) at Hirna. The ADF value was ranged 30.4% (local bean) to 36.6% (Tesfa) and 34.9% (Tesfa) to 42.4% (Mesay) with lignin content of 5.8% (CS-20-DK) to 6.5% (Tesfa) and 8.3% (CS-20-DK) to 6.5% (local bean) at Haramaya and Hirna, respectively. 335 bean varieties for dry matter degradability both at Haramaya and Hirna (Table 5), organic matter (Table 6) and Neutral detergent fiber degradability (Table 7) at Haramaya. In sacco Dry matter, Organic matter and Neutral detergent fiber Degradability Except for the rate of degradability there was significant difference (P<0.001) among the faba Table 4. Neutral detergent fiber, acid detergent fiber, acid detergent lignin, cellulose and hemicellulose contents of faba bean varieties at Haramaya and Hirna sites Varieties Tesfa Bulga-70 CS-20-DK Mesay Local bean Significance Overall mean ± SEM CV a, b, c, d NDF (%) HU 40.9a 34.4c 33.1d 35.7b 33.7cd *** 35.56 ±0.28 1.78 HI 39.6c 47.1b 47.1b 51.5a 46.2b *** 46.30 ±0.30 1.45 ADF(%) HU 36.6a 30.6b 31.6b 31.9b 30.4b *** 32.22 ±0.45 3.12 HI 34.9c 39.8ab 38.8abc 42.4a 37.6bc * 38.7 ±1.04 6.01 ADL(%) HU 6.46a 5.46b 5.76ab 6.19ab 5.83ab * 5.94 ±0.20 7.02 HI 6.8ab 7.5ab 8.3a 7.9ab 6.5b * 7.4 ±0.39 11.80 Cellulose(%) HU HI 30.1a 28.1d cd 25.2 32.7b 25.8bc 29.4cd b 25.9 35.5a d 24.6 30.7c *** *** 26.32 31.28 ±0.17 ±0.44 1.46 3.12 Hemicellulose(%) HU HI 4.39a 4.7c cd 3.76 6.7b 1.56bc 9.0a b 3.68 8.2ab c 3.23 8.4a *** *** 3.32 7.4 ±0.05 ±0.28 3.28 8.41 Means followed by different superscripts in column are significantly different; SEM= standard error of mean; CV= coefficient of variation; NDF= neutral detergent fiber; ADF= acid detergent fiber; ADL=acid detergent lignin; *P<0.05; **P<0.01; ***P<0.001;HU = Haramaya University; HI= Hirna. Table 5. In sacco dry matter degradability parameters of faba bean varieties at Haramaya and Hirna sites Varieties Tesfa Bulga-70 CS-20-DK Mesay Local bean Sig. level Overall mean± SEM CV (%) a (% DM) HU HI 48.5b 48.5a 49.6b 44.6b 57.3a 41.2c 55.7a 34.8d 57.8a 48.0a *** *** 53.78 43.42 ±0.80 ±0.36 2.57 1.45 B (% DM) HU HI 27.3b 24.7e 31.0a 31.0c 23.2c 36.0b 26.0bc 41.0a 23.3c 26.7d *** *** 26.16 31.88 ±0.67 ±0.24 4.46 1.28 c (% DM) HU 0.076 0.094 0.090 0.074 0.10 ns 0.0868 ±0.004 10.43 HI 0.103 0.130 0.075 0.085 0.060 ns 0.0906 ±0.003 7.76 TL (hr) HU 5.62ab 4.00b 7.26a 5.41ab 6.85a *** 5.828 ±0.46 13.72 HI 7.20b 3.48c 2.97cd 2.04d 8.24a *** 4.786 ±0.2 7.16 PD (hr) HU 75.8c 80.6b 80.5b 81.7a 81.2ab *** 79.96± 0.13 0.29 HI 73.2d 75.6b 77.2a 75.8b 74.7c *** 75.3 ±0.10 0.23 ED (% DM) HU HI 53.9c 57.3b 58.8ab 62.1a 63.5 ab 47.9cd 60.5 b 44.7d 65.6 a 51.3c *** *** 60.46 53 ±1.03 ±0.86 2.97 2.81 a, b, c d means followed by different superscripts in column are significantly different (P<0.001). HU = Haremaya University; HI= Hia; a = rapidly degradable; b= insoluble, but potentially (slowly) degradable component; c= the rate of degradation of ‘b’ component; TL = lag time; PD=potential degradability (a+b); ED= effective degradability at outflow rate of 0.03h-; S.E.M= standard error of mean; C.V= coefficient of variation; S.L= significance level; *** =P<0.001; ns= non significant. Table 6. In sacco organic matter degradability parameters of faba bean varieties at Haramaya sites Varieties a (% DM) b (% DM) c (% DM) TL (hr) PD (hr) ED (% DM) Tesfa 49.6c 24.7b 0.070 5.20b 74.3c 53.7b a b a a Bulga-70 63.5 27.7 0.080 8.11 88.2 67.9a d a b bc CS-20-DK 41.1 37.6 0.167 3.65 78.7 60.4ab a b a bc Mesay 60.1 22.0 0.097 8.13 77.7 67.4a b b b ab Local bean 55.0 27.8 0.096 5.87 82.8 63.4ab Sig. level *** *** ns ** *** * Overall mean± SEM 53.86±0.82 27.96±1.24 0.1±0.004 6.2±0.36 80.34±0.99 62.56±2.41 CV (%) 3.44 9.94 10.74 13.11 2.74 8.61 a, b, c, d means followed by different superscripts in column are significantly different. a= rapidly degradable; b= insoluble, but potentially (slowly) degradable component; c= the rate of degradation of ‘b’ component; TL = lag time; PD=potential degradability (a+b); ED= effective degradability at outflow rate of 0.03h-1; S.E.M= standard error of mean; C.V= coefficient of variation; S.L= significance level; *** =P<0.001; **= P<0.01; *= P<0.05; ns= non-significant. 336 Table 7. In sacco neutral detergent fiber degradability parameters of faba bean varieties at Haramaya Varieties a (% DM) B (% DM) c (% DM) TL (hr) PD (hr) ED (% DM) Tesfa 20.22a 35.42b 0.057 4.91b 55.64b 23.99b a a b a Bulga-70 27.82 46.94 0.087 4.63 74.76 43.80a b a b b CS-20-DK 7.90 47.61 0.190 4.42 52.44 39.30a a ab b b Mesay 19.66 39.07 0.080 4.03 53.73 29.66b a b a b Local bean 20.68 37.50 0.085 7.50 58.18 29.91b Sig. level *** *** ns ** *** *** Overall mean± SEM 19.3±1.49 14.3±1.4 0.1±0.005 5.1±0.41 59±1.80 33.3±1.12 CV (%) 17.28 7.59 16.06 17.8 6.84 7.53 a, b, means followed by different superscripts in column are significantly different (P<0.001, 0.01) a= rapidly degradable; b= insoluble, but potentially (slowly) degradable component; c= the rate of degradation of ‘b’ component; TL = lag time; PD=potential degradability (a+b); ED= effective degradability at outflow rate of 0.03h-1; S.E.M= standard error of mean; C.V= coefficient of variation; S.L= significance level; *** =P<0.001; **= P<0.01; ns= non significant DISCUSSIONS Leaf to Stem Ratio, Grain Yield, Harvest Index and Potential Utility Index According to Mushtaque et al (2010) LSR decreased as the plant advanced in maturity. This is also in agreement with the present study; some of the faba bean varieties (“Mesay” and local bean) which take longer time to mature relatively had lower LSR. Also the grain yield of these faba bean varieties was similar to the figure previously reported by (Lulseged and Jemal 1989). In this study the high grain producing varieties “Mesay” at Haramaya and “Mesay” and “Tesfa” at Hirna had significantly higher in HI than most of other varieties. On the other hand, the poor grain producing varieties like “CS-20-DK” and local bean at Hirna had significantly lower (P<0.05) HI most of the varieties. The PUI result of the current study indicated that there are varieties, which combined high grain yield, IVDMD, SDMY and PUI. This shows the possibility of selecting for faba bean varieties for straw yield and straw quality without marginalizing grain yield. Measurement of PUI of a crop is a good parameter in this respect as it integrates grain yield and digestible dry matter yields from the residues (Fleischer et al 1989). The PUI result of the current study was lower than that of the PUI of different varieties of tef straw (Seyoum et al 1996). Straw Dry Matter Yield Straw to Grain Ratio and In Vitro Dry Matter Digestibility The SDMY in the current study varies from 3.44 t/ha to 7.11 t/ha. The varieties gave higher SDMY value than reported for different varieties of barley in the highland of Ethiopia (Seyoum, 1995) and stover yield of maize (Adugna et al 1999) and the varieties were similar in SDMY with the different oat varieties (Fekede, 2004). “Tegenech” was higher in both grain and straw yield. This indicates that the possibility of selecting varieties that combine high grain yield with high straw yield. Unlike the result of harvest index, poor grain producing varieties (CS-20-DK) had significantly higher (P<0.001) straw to grain ratio. In this study the IVDMD value of the faba bean ranges from 65% to 73.8%. The result of the current study showed higher value of IVDMD than that of native grass hay (Getachew, 2002), maize stover (Leask and Daynard 1973), wheat straw (Coxworth et al 1981), oats and vetch mixture (Berhanu, 2004). Meissner et al (2000) reported that in vitro digestibility values greater than 65% indicate good nutritive value and those values below this level result in reduced intake due to lowered digestibility. The values obtained in the present study were higher than this critical level. Chemical Composition The current study of CP is similar to the value reported by Kossila (1984), which ranged between 10-15%. The CP content of faba bean in this study was higher than that of maize stover (Adugna et al 1999), cereal straws (Seyoum and Zinash 1989). The mean CP content was higher than the critical value of 7% for normal rumen microbial action and feed intake (Van Soest 1982). According to Nsahlai et al (1996) roughage feeds with CP content of 9.92 to 15.2%, 6.6 to 9.1% and 3 to 6.5% were classified as high, medium and low quality roughage feeds, respectively. The faba bean varieties evaluated in this study could be classified as high quality feed with respect to their CP contents. Neutral Detergent Fiber, Acid Detergent Fiber and Acid Detergent Lignin The NDF content of faba bean in this study lies within the range of 33.1% to 51.5%. This result was lower than the critical level of 55-60% which was reported to decrease voluntary feed intake and feed conversion efficiency due to longer rumination time (Shirley 1986). According to Singh and Oosting (1992), these faba bean varieties are average quality feed since their NDF lies between 45-65%. The ADF results of this study were lower than the values reported for faba bean straws (Luelseged 337 and Jemal 1989), cereal and oil straws (Seyoum and Zinash 1989), tef straw (Seyoum and Zinash 1996) and maize stover (Adugna et al 1999). The lower value of ADF in this study could be indicative of its better digestibility than other straws. Generally, the lignin contents of the faba bean varieties in this study lie within the range of 5.0 to 6.5%. Lignin is a compound, which attributes and resistance to plant tissue, thereby limiting the ability of the rumen microorganisms to digest the cell wall polysaccharides (Reed et al 1988). The polysaccharides of the cell wall become more digestible once the lignin has been removed (Jones and Wilson, 1987). Therefore, those varieties, which have higher lignin content could have low digestibility than the lower lignin containing varieties. In Sacco Dry Matter, Organic Matter and Neutral Detergent Fiber Degradability In the current study the faba bean dry matter degradability had higher value of a, c, TL, PD and lower value of b than that reported by BrunoSoares et al (1999) for the same parameter of faba bean DM degradation. Except for a faba bean straws had higher value of b, c, PD and ED than tef straw and maize stover which are reported by Solomon (2001) and Adugna et al (1998), respectively. The current study of OM degradability constants of faba bean were higher in a, c and ED than that of tef straw reported by (Solomon, 2001). Moreover, the NDF degradability result of the current study was higher in a, b, c, and PD and ED value than the respective result of faba bean straw by Bruno-Soares et al (1999), tef straw (Solomon 2001). CONCLUSION The varieties Tesfa, Mesay, and local bean were identified to have high grain yield and SDMY. The varieties with relatively high PUI value were Bulga-70, Mesay and local bean. In addition, the result showed some significant varietal difference in grain yield, straw dry matter yield, potential utility index, harvest index, chemical composition and degradability. Moreover, there are varieties which had significantly higher value in both grain yield and straw yield. This indicates the possibility of selecting faba bean varieties that combine better grain yield with desirable straw parameters. In the current study the different varieties of faba bean had higher CP, lower fiber contents and relatively higher in sacco degradability than cereal straws and maize stovers. However, Tesfa, Mesay and local bean were identified to have high grain yield and better nutritive value than Bulga-70 and CS-20DK. ACKNOWLEDGMENTS First and foremost, we are greatly indebted to Haramaya University for sponsoring this research project. 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