The Capacity to Compare Capacitors and Cell Membranes Objectives: Compare a cell membrane of a neuron to an RC circuit. Introduction: A capacitor can not produce new electrons – it only stores them. The movement of these stored electrons is important in providing short bursts of energy. In biology, the movement of charges is important in nerve impulses. Test you knowledge with a Capacitor Quiz: http://electronics.howstuffworks.com/capacitorquiz.htm If you need a review about active transport in the cell membrane, watch the following video and answer the questions? http://www.youtube.com/watch?v=fxOIcWwdl-o 1) How many sodium ions are removed from the cytoplasm by one protein molecule? 2) Does the movement of sodium ions require energy? 3) How many potassium ions enter the cell by one protein molecule? Use the following graphics to answer the question below: How does a cell membrane compare to a capacitor? (Include the structure acting as the dielectric). An RC (resistor, capacitor) equivalent circuit model is a good model for a patch of passive neural membrane. The capacitor represents the fact that cellular membranes are good electrical insulators. The battery represents the sodium-potassium pump that acts to hold the electrical potential of the inside of the cell below that of the outside. This voltage difference is called the resting potential of the neuron. The resistor represents the leakage of current through the membrane 1.5V 1.5V Capacitor Lamp Activity: Materials: Two 1.5 V batteries in series, 1 F capacitor, single 2.5 V light bulb, wire leads, digital volt meter, switch Procedure: Charging Capacitor 1) Hook batteries, light bulb, and capacitor in series. See diagram. LEAVE one lead disconnected at a battery terminal (switch). Make sure the capacitor’s polarity is inserted correctly (negative toward negative part of battery). 2) Using leads connect the DVM (digital volt meter) across the capacitor. Set DVM to 20 V DC max scale. 3) CLOSE the circuit by attaching the lead to the battery or closing the switch. 4) Observe the brightness of bulb and describe:______________________ 5) Observe the voltage on DVM when it stops: _______________ Capacitor is now fully charged 6) Disconnect both leads from battery. 7) Momentarily (for 1 second) connect the leads then disconnect them and observe light bulb and DVM (See diagram below). Disconnect, and reconnect 3-4 times, letting the bulb stay on for 1 second each time. Light bulb:_________________________________________ DVM:_____________________________________________ Capacitor Lamp Questions: 1. How does connecting the leads together resemble a sodium ion channel in a cell membrane? 2. How does disconnecting the leads resemble a sodium ion channel? Can you relate this to action potential? 3. What biological process does the charging of the capacitor represent? 4. What does the battery represent in the neuron? Extension: Using a battery, switch, capacitor, and potentiometer, can you show how the action potential increases during depolarization and then decreases during repolarization?