NEW
HS All-in-one Fluorescence Microscope
BZ-9000 Series
Truly-revolutionary, New-generation
Fluorescence Observation
Advent of High-End Model that Provides
High Sensitivity and High Resolution
ALL- IN - ONE FLUORESCENCE MICROSCOPE ELIMINATES NEED FOR DARKROOM
HS All-in-one Fluorescence Microscope
that enables Quick and Easy Observation
into Deep Layers
“BIOZERO” was designed by reviewing the basics of fluorescence observation in terms of
factors required for fluorescence microscopes from the zero point.
Furthermore, the High Standard (HS) ergonomic observation method of BIOREVO brings
about a “revolution” in the history of fluorescence microscopes, resulting in the
remarkable development of fluorescence microscopes.
2
CON TEN TS
STA N D A R D EQU IP M EN T
P4
No Need For A Darkroom NEW
Has A Six-mount Electronic Revolver
New useful “F-OPT” structure enables fluorescence observation in any location.
Monochrome
Colour Switching Camera [Industry First]
P6
NEW
Monochrome CCD provides remarkably improved sensitivity and resolution.
Wide versatility enabling 2-way (monochrome/colour) switching.
Navigation System [World First]
P8
NEW
Revolutionary observation application that leads you to an optimum observation
point with a single click.
P10
Haze Reduction Function
Eliminates fluorescence blurring, enabling visualisation of weak fluorescence signal.
Quick Full-focusing Function
P11
NEW
New function for clarifying out-of-focus afterimages.
Image Joint Function
P12
Joints wide-view images into high-quality image at high speed.
O PTION
Dynamic Cell Counts
NEW
P16
New function using new algorithm to separate overlaid “cells” and count individual cells.
Multidimensional Time-Lapse
P18
Captures images to find a change in the cell in chronological order.
Shooting points can be moved and observation conditions can be changed.
Real-Time 3D Module
P20
Advanced function that enables accurate observation of local expression,
to report observation results to a third party.
Measuring Module
P21
Enables easy quantification of observation results.
3
All-in-one Fluorescence Microscope
NEW STRUCTURE
Fully electronic control motorized components simplify setup and control
[Folded Optical Structure]
F-OPT STRUCTURE
The optical path for the image-formation optical system
and transmitted illumination is folded with mirrors, and
incident illumination and the image-formation optical
system are horizontally located at optimum angles. This
structure provides the space-saving design for the
high-quality infinite optical system.
1
Phase-contrast slit electronic
switching mechanism NEW
For phase-contrast observation, the
phase-contrast slit is automatically
switched according to magnification of
the objective lens (between 20x and 40x).
6
2
1
Electronic dimmer filter NEW
Excitation light dimming ratio can be
changed by external remote control.
The dimming ratio can be selected in 5
levels by colour, according to expression
intensity and discolouration conditions of
multi-dyed specimens. Safety design that
can reduce damage to specimens.
Transmitted illumination
3
Electronic shutter
When you suspend observation, the shutter
for the excited light is automatically closed.
This prevents accidental fading of
fluorescent specimens.
4
Electronic XYZ axes stage
The electronic stage moving along the X,
Y, and Z axes supports the field and focus
adjustment in the "Black Space", which is
cut off from the outside world.
5
Electronic filter turret
Four filter cubes (excitation filter, absorption
filter, dichroic mirror) can be mounted and
switched by electronic control. The filters
can be changed not only from a PC but also
from the unit itself.
4
Objective lens
6
2
Electronic aperture control
During bright-field observation, aperture
can be changed by remote control..
4
Fluorescent incident illumination
3
5
Image-formation optical system
4
BLACK SPACE
No Need for a Darkroom
The built-in “black space” permits fully electronic control
from the outside
Conventional fluorescence observation requires operation in a
darkroom in order not to lose the contrast to the background
other than the signals. With the BZ-8000 Series, however, such
operations are performed inside the unit, allowing the unit to be
installed anywhere. The picture-taking operation in the built-in
“Black Space” can be fully controlled with the electronic XYZ
axes stage, electronic optical zoom, electronic shutter, and
electronic filter turret.
NEW
Six-Mount Electronic Revolver
Objective lens can be switched
according to the observation
purpose
Space-Saving
About 40% space reduction
By reviewing the fundamentals of the internal structure, the foot
print has been reduced to about half, which allows for a
space-saving installation even in the room or the laboratory.
Microscope
Power
supply for
a mercury Power
Camera
lamp
supply for
a halogen
lamp
Light source
Control box
Hand switch
Focus
handle
Configuration of a conventional system
Up to six types of objective lenses
(2x to 100x) can be mounted. The
lens can be switched over with a
single click on the PC control
screen, thus enabling smooth
operation.
❙ Collision preventive function for lens switching operation
(Automatic move/return to Z-axis)
❙ Suppressing vibration and visual field displacement during lens switching operation,
resulting in stable operation
BZ-9000 All in one
* Corrects visual field displacement with the electronic XY stage during lens switching operation.
Great Versatility
Improved Basic Functions
Bright-field, fluorescence, and phase-contrast observation
Optimized cooling system
With the compact body, the BZ-9000 provides three observation
modes. During observation mode changeover, light source,
exposure time and filter setup changes are easy with a single
click of the mouse.
The remaining heat resulting from the
all-in-one structure is completely
eliminated by the optimized design
of the separation of the upper/lower
chassis and the cooling fan. In the
specimen room, in particular, the
temperature difference from the ambient temperature is limited
to +3°C or less even when the light is interrupted.
Bright-field
Fluorescent
Phasecontrast
Antivibration design
Various specimen types are acceptable
You can use various types of specimens such as glass slides,
plastic petri dishes, multi-well plates, and glass bottom dishes.
The BZ-9000 can cancel the influence
of vibration of the cooling fan by using
a spring of the optimum design.
The weight of the upper chassis has
been reduced to lower the centre of
gravity, so that influence of external
vibration can be minimised.
Long-life mercury lamp without need for axis alignment
The lamp life has been improved to 2,000 hours, or five times
longer than that of conventional lamps.
5
Monochrome High Sensitivity/
Colour Switching CCD Camera
NEW STRUCTURE
Fully electronic control motorized components simplify setup and control
The BZ-9000 is equipped with a monochrome CCD suitable for high sensitivity, long wavelength and high-speed shooting. With the
electronic colour filter insertion mechanism, the camera enables easy switching to colour observation mode. The BZ-9000 covers a
wide range of applications, from weak fluorescence signal processing and infrared image capturing to HE dyeing pathological
diagnosis, even with a single unit.
Mitochondria
(100x objective lens)
MONOCHROME
(Weak fluorescent image)
High Sensitivity
High Resolution
Long Wavelength
High Speed
High Gradation
Switchable
with a single click
2 WAY
Kidney glomerulus
(40x objective lens)
COLOUR
(HE dyed image)
Colour Reproducibility
3CCD Image Quality
6
With its high sensitivity characteristic, the BZ-9000 can capture
images under weak excitation light in the monochrome shooting
mode. This feature is advantageous in reducing damage to
specimens. Also, the BZ-9000 enables shooting of an infrared ray
wavelength (Cy7, etc.). This feature is suitable for capturing images
of a cell with expression in a deep layer.
Relative Response
High Sensitivity and Long Wavelength
BZ-9000 wavelength/
sensitivity characteristics
Conventional wavelength/
sensitivity characteristics
Wave Length (nm)
High Resolution
The BZ-9000 provides high-resolution images that clearly express minute profiles in nuclei. In the colour shooting mode, the 3CCD system
provides excellent colour reproducibility, offering high-definition images.
Conventional image
(Cattle pulmonary artery)
BZ-9000 image
(Cattle pulmonary artery)
High-Gradation 12-bit
Random Noise Eliminating Function
Through high-accuracy quantification, the BZ-9000 enables
high-precision measurement of expression quantity in 4,096
gradation levels.
Eliminates noise by using several frame data through the
dedicated circuit with a frame buffer.
Image with noise
Image after noise elimination
Conventional gradation levels
256 gradation levels
4096 gradation levels
High precision
High-Speed Shooting [100 frames/sec. max.]
Provides on-chip binning (2 x 2, 4 x 4, 8 x 8) function, enabling shooting at 100 frames/sec. max. * When “8 x 8” binning is used
7
Navigation System
NEWLY-DEVELOPED
Revolutionary observation application that leads you to an optimum observation point with a single click
With a click on a desired point on the low-magnification lens image navigation screen, the high-magnification lens preview point moves
to the specified coordinates through electronic control, and a high-magnification image is displayed. The BZ-9000 achieves a new
observation style that moves the view point with a click on a target point on the navigation screen, without having to search for a target
point on the XY stage.
This navigation system improves visibility of the whole specimen, thus supporting effective observation and shooting. Also, this system
can capture tiling images of the whole specimen. (* Refer to the image joint function described later.)
Find an observation point in a navigation image (wide-view image), and click the target point.
Preview image
Navigation image
Easy finding
of
view point
A
B
Click
* Joint image can be inserted.
Navigation image: Ovary (equivalent to 0.5x)
NAVIGATION SYSTEM APPLICATION
Easy positioning with oil-immersed lens
The view point can move to desired coordinates with the
high-magnification oil-immersed lens. Replacement with a
low-magnification dry lens for view finding is not required.
Quick Observation of HE Dyed Section
Clicking on a periosteum regeneration part in the whole image of
skull tissues enables quick high-magnification observation.
Click
High-magnification preview image
Click
Navigation image
Navigation image
High-magnification preview image
8
Click
High-magnification preview image
Electronic control enables movement
to a desired observation point with a
single click
Before Movement Preview Screen (Target at 20 x magnification)
A
Navigation image
A
After Movement Preview Screen (Target at 20 x magnification)
B
Navigation image
Type of area frame
Current preview area
B
Indicates a current observation area in a visual
field range.
1
Stage position memory area
Indicates a stored XY coordinate position.
Captured area
Indicates an area that has been captured.
Extraction of Target Image from Multi-dyed Cell
Overlay Images
Dual Displays for Macro Image
Visibility Improvement
Enables a cell that can be identified only by overlay processing
to be observed in the navigation image at a glance.
Navigation image and preview image (high-magnification image)
can be simultaneously shown on dual displays.
Rat kidney
Navigation image
(Observation of Cy3 + GFP overlaid images: Yellow part)
Click
High-magnification preview image
Navigation image
High-magnification preview image
9
Haze Reduction Function
Eliminates fluorescence blurring, enabling visualisation of weak fluorescence signal.
Quickly eliminates an out-of-focus image (fluorescence blurring) on the objective lens, and improves the image to a high-contrast
image. The “Real-Time Haze Reduction” function to quickly eliminate fluorescence blurring is also provided on the preview screen.
Haze reduction image
Raw image
Mouse embryo (before implantation) (Courtesy of Mr. Atsushi
Sawada of Laboratory for Embryonic Induction, RIKEN Center
for Developmental Biology)
The blurring can be
eliminated immediately
Click
This image was taken using the normal method with a fluorescence
microscope. The target fluorescence signals cannot be recognized
clearly due to fluorescence blurring.
The haze reduction function removes the light diffused on the unfocused
surface (blurring). This allows accurate recognition of the location of the
fluorescence signals.
HAZE REDUCTION IMAGE SAMPLES
Raw image
Bacteria
Raw image
ACTUAL ACHIEVEMENTS
Colon cancer cell (HT29)
Actual achievements obtained by using
the Biozero haze reduction function
were introduced in the U.S.A.'s science
magazine "Nature Immunology".
Title:
Mechanistic basis of pre -T cell receptor-mediated
autonomous signaling critical for thymocyte
development.
Researcher:
Yokohama Institute of Physical and Chemical Research
Immunology and Allergy Science Research Center
Doctor Masaru Yamazaki of Immune Signal
Research Group
Click
Haze reduction image
Click
Haze reduction image
Reference Magazine:
Nature immunology volume 7 number 1 January 2006
P74“BZ-8000 (KEYENCE) was used for deconvoluted
fluorescence imaging”
Raw image
Haze reduction image
Intracellular location of GFP-labeled pre-T cell receptor
10
Quick Full-Focusing Function
NEW
New function clarifies unfocused afterimages
The quick full-focusing function is a revolutionary new function that can
synthesise a full-focus image simply by turning the mouse wheel. The
BZ-9000 captures several images while moving the objective lens focal
position with the electronic Z-axis stage that is linked with the mouse wheel.
The BZ-9000 extracts only a focused point among group images captured,
to synthesise a full-focus image in real time. This function is advantageous in
storing an observation result as an “image” instead of a “sketch”, so that the
observation result can be reported exactly to a third party. Also, the BZ-9000
enables quick and accurate measurement even with time-consuming
observation items (e.g. nerve axon elongation, chromosome count control).
Full-focus image
Drosophila
Simple composition
just by turning mouse wheel
IMAGE SAMPLES
Just by turning
mouse wheel
Full-focus image
Just by turning
mouse wheel
Mouse dendrite
Measurement of axon length with free length measurement function
Full-focus image
Just by turning
mouse wheel
Rat retina blood vessel
Full-focus image
Zebrafish
Courtesy of Mr. Tsutomu Nakahara of Department of Molecular Pharmacology,
Kitasato University School of Pharmaceutical Sciences
11
Image Joint Function
Connects images with high speed and quality
Micro images and macro images (e.g. brain sections and myocardial cells) may be mutually related and shot. In this case, shooting
with even brightness is required because errors in immuno-staining and fluorescence expression affect the analysis result. This "image
merge function" has solved these problems to provide image processing that can be performed at speeds over eight times faster than
conventional speeds.
Original image
Image created by image joint function
Wide-view image of multi-dye brain section
Image Joint
Click
10x object lens, 28 images
Synthesising
full-focus
image from
slice images
Eliminating
fluorescence
blurring
Overlaying
multi-dyed
images
Quick and easy multi-functional tiling
Shading cutting
The image merge function uses the shading canceller to cut uneven contrast around a view resulting from uneven light intensity and lens
aberration, allowing natural image linking.
Conventional software
BZ-9000 image joint
Conventional software
The shade at the seam is removed by
the shading canceller.
Uneven light intensity causes a shade
in the seam.
He chromatic figure
Uneven light intensity causes a shade
in the seam.
BZ-9000 image joint
Fluorescent image
The shade at the seam is removed by
the shading canceller.
High-speed mapping using XY coordinate information
The image merge function links XY coordinate information with the image information shot by Biozero and performs high-speed mapping.
Our original image analysis algorithm has realized high-speed image processing that is over eight times faster than conventional image
processing.
12
Automatic Range Setting for Image Joint
NEW
When moving the table on which a specimen is mounted, find the maximum coordinate position (most protruding point) of the specimen
and click on the specified position. Then, click the [Photo] button. With conventional systems, a wide-view image capturing range cannot
be identified, causing omission of an intermediate image. However, this function can accurately capture all images (max. 30 (vertical) x
40 (horizontal) pieces: 1200 pieces in total) without a failure.
Whole image of specimen
BZ-9000 image joint
Click SET on specimen periphery.
Synthesis of whole specimen image
Click
Top:
Maximum coordinate position
Click
Click
Left:
Maximum coordinate position
High-speed scan
Image joint
Right:
Maximum coordinate position
Click
Bottom:
Maximum coordinate position
4x objective lens, 25 images
High-resolution, wide-view image of ovary
IMAGE SAMPLES
Original image
10x objective lens, 42 images
BZ-9000 image joint
Wide-view image of spleen
BZ-9000 image joint
Wide-view image of kidney section
Image joint
Click
Original image
10x objective lens, 45 images
Image joint
Click
13
Various Functions Available with Mouse
Five mouse operations available with electronic all-in-one system
Optimum adjustment of the microscope unit and peripheral units required for microscopic observation
requires operator proficiency. The electronic all-in-one system of BIOREVO enables you to execute basic
operations simply with a mouse. This system provides an ideal operation flow that allows anyone to easily
maximise the microscope’s potential.
Observation Applications
Observation mode switching
Multidimensional task
Monochrome
Colour switching (p.6)
Channel switching
Objective lens switching
Binning setting
Auto focusing
Black balance
Real-time haze reduction
Auto calibration scale
Navigation function (p.8, p.9)
Illumination ON time
User setting storing/reading
File save setting
Electronic Z-axis stage operation system
Electronic XY stage operation system
XY/Z-axis stage coordinate preset
Capturing images for image joint
Illumination adjusting slider
Fluorescent shutter
auto-OFF function
1
Mouse wheel
setting indication
One-click shooting
Focal point adjustment Interlocking with the Z-axis electric motor
Turning the mouse wheel
enables you to adjust a
microscope focus.
Far
Near
Quick full-focusing function (p.11)
4
XY stage movement Interlocking with the XY axis electric motor
Mouse dragging provides
movement in any XY
direction.
Drag
Electronic focusing for the Z axis
2
Observation magnification change Interlocking with the optical electric zoom
Turning the mouse wheel
enables you to change
theoptical magnification of
the microscope.
1.0x
3.0x
5
Preview screen switching Interlocking with the electric filter turret and fluorescent shutter
Only clicking CH1 to CH4 on the viewer screen allows you to change
the fluorescent filter, turn on/off the fluorescent shutter, and switch to
bright field observation. This clicking also allows you to change the
preset exposure time.
Magnification zooming
Red
3
Turning the mouse wheel
allows you to change the
exposure time (shutter
speed) of the cooling CCD
camera.
Fast
Blue
Slow
Mitochondria
Exposure time adjustment
14
Green
Exposure time adjustment Interlocking with the CCD camera shutter speed
Cytoskeleton
Core
Optimised Camera Settings
with A Single Click
Easy-to-Use Microscope Designed for Everyday Use
CH1
Phase-contrast
image
CH1
Click
4-channel images captured in different observation modes are displayed on 4-split preview
screens, and the images are overlaid.Switching multi-dyed specimen images by the colour.
Overlay Switching between phase-contrast observation and fluorescence observation Overlay
This function provides the ideal operation flow that enables quick and easy overlaying on the
preview screen, without requiring image processing with commercially-available software.
COMPARISON OF PROCEDURE WITH CONVENTIONAL PRODUCTS
Conventional Microscope
Conventional procedure
CH2
GFP image
CH2
Operation steps
(To change from a bright-field image to a fluorescent image)
These items must be set one at a time.
Click
A click of the
screen changes
the display
setting.
3
2
1 Turn off the halogen lamp for the transmitted illumination.
5
7
4
2 Open the shutter for the excited fluorescent light.
3 Select the fluorescent filter according to the target colour.
1
6
4 Change the optical path from the eyepiece to the camera port.
5 Adjust the camera exposure time (In the automatic mode, adjust the metering method.)
6 Adjust the camera focus again by watching the monitor.
CH3
DAPI image
7 Apply the following camera settings one at a time.
1Contrast correction→2Black balance→3Real-time filter→
4ISO sensitivity adjustment
CH3
Channel settings
BZ-9000
Click
(The following setting memory is available.)
❙ Observation mode (Fluorescence, Bright-field, Phase-contrast)
❙ Exposure time setting
CH4
Overlay image
❙ Dimmer filter setting
❙ Camera gain
❙ Preview speed setting
❙ Binning
❙ Black balance setting
CH4
❙ Haze reduction setting
All of these steps are completed with
Click
a single click of the mouse
❙ Overlay blend ratio
❙ Pseudo colour
❙ Comment (Filter name)
BZ-II Image Analysis Application [BZ-H2A]
Grouping Image Book
Displays a list of Z-stack and time-lapse group images, as well as a single image.
Group image batch processing function
Z-stack image
Z-stack
Overlay
Z-stack
Overlay
Full-focusing
Z-stack image and time-lapse image
are recognised as group images.
❙ Haze reduction
❙ Overlay
❙ Full-focusing
Batch processing for the above
functions is enabled. Easy-to-operate,
useful function.
Slice viewer
Group images can be
quickly switched with
mouse wheel operation,
like leafing through a
photo album.
Turning the mouse wheel
Time-lapse image
15
Dynamic Cell Count BZ-H1C
(Optional)
NEW
Cell Separation and Extraction
The Keyence-original “cell separation method” is used to separate and count individual cells. This
method extracts cells by separating individual cells based on a “change in brightness level” that
provides abundant information, instead of the binary edge extraction method. This method enables
separation of highly-agglutinative, non-circular cells because edge information is not required. If
visual judgement by an operator is required for identification of an individual cell because of a
complicated cell shape, it can be separated or combined through manual correction, so that the
count accuracy can be improved. The operation flow is easy to understand, which ensures smooth
operation and assists you in speedy and accurate counting.
Raw image
Cell separation processed image
Click
A separated cell counts as an individual.
Separation level changing slide bar
Conventional binary processing
Cell separation level
can be changed using
the slide bar.
Cannot recognise each cell as an individual.
Correcting function
16
Elimination of minute particles
Area joint
Eliminates particles in small areas.
Joints several binary-processed areas.
Fill-in
Elimination of specified area
Fill in a black hole with white.
Eliminates a specified unnecessary area.
Enlarge
Image correction
Enlarges a binary-processed area.
Corrects an image manually.
Reduce
Edge extraction
Reduces a binary-processed area.
Extracts an edge of the clicked target.
Colour Extraction
Extracts and counts specified colours on the screen. Based on colour information, stable extraction is enabled even with
weak expression.
Colour extraction processed image
Click
Only the red area of necrosis can be extracted.
Brightness Level Extraction (with circular separation function)
Automatically recognises a brightness level of an image, and extracts a specified area through binary processing. This
function enables separation and counting of agglutinative cells, in combination with the circular separation function.
Circular separation processed image
Circular separation
Click
Automatically separates
cells in the circles.
Measurement Data
Histogram
Measurement data list
By linking a measurement data number
with the number indicated on the
screen, the relevant object can be
highlighted.
Brightness level sum value, area, periphery length, and long/short
diameters can be displayed.
Auto-calibration
A target image can be displayed in its actual size, based on the
shooting conditions embedded in the image.
Minimum value, maximum value, value range, average and standard
deviation can be displayed.
17
Multipoint Time-lapse BZ-H2TL
(Optional)
A bright-field image, fluorescence image and phase-contrast image are captured in chronological order at a preset time interval. The BZ-9000
stores XY coordinates on up to 30 points, and captures target images while moving the XY stage. A multi-colour image and Z-stack image
at each point can be also captured. This function provides several cell images through a single experiment, resulting in remarkable improvement
in experimental efficiency. Excitation light is automatically turned OFF other than during the shooting operation. The BZ-9000 is designed to
ensure the longest specimen life by reducing phototoxic effect.
Multi-colour, Z-stack Time-Lapse Function
Multi-point, Multi-colour, Z-stack Time-Lapse Function
The multicolour Z-stack time-lapse function obtains scanning
images in the Z-axis direction in chronological order while
automatically selecting the filter turret matching the multi-staining
procedure sample.
The multipoint/multicolour Z-stack time-lapse function obtains
images while moving the multicolour Z stack time-lapse function on
XY coordinates by up to 30 points.
n min
n min
(n+1) min
n min
n min
Z-stack
Z-stack
Z-stack
n min
Z-stack
Z-stack
Z-stack
Z-stack
Z-stack
Z-stack
Z-stack
Z-stack
Z-stack
t1-XY1
t1
n min
n min
t2
Time
t1-XY2
t1-XY3
Up to 30 points
Multi-point, Joint, Time-Lapse
t2-XY1
t2-XY2
t2-XY3
Time
Up to 30 points
* Standard feature (joint, multi-point), when time-lapse function is not used.
Enables shooting while moving shooting points, in addition to multi-colour and Z-stack shooting.
Shooting method: Multi-point, Time-lapse
Shooting method: Joint, Multi-point, Time-lapse
Shooting method: Joint, Time-lapse
Captures images on preset coordinates in
sequence.
Captures images from preset coordinates by the
number of joints in sequence.
Captures images in the area including all preset
coordinates (40 (Horizontal) x 30 (Vertical) images max.)
* Up to 32767 pixels can be jointed.
❙ This function can be linked with another function (Z-stack).
XY coordinate memory screen
While monitoring the preview screen, click on [SET] at a shooting point.
Through repetition of this procedure, XY coordinates are stored in memory.
If “Z-axis link” is checked, the Z-axis position at which [SET] is clicked is
stored in memory. A specimen with height differences can be moved in the
XY direction while being kept in focus.
Storing XY coordinates on up to 30 points
18
Best Focus Auto Extraction Function
This function is very useful for extracting best focus images from a large number of images that have been captured with the Z-stack function
and compiling them into time-series group images (time-lapse images) at a high speed. This function provides a great advantage in work
efficiency improvement, because visual searching for focused images from large-volume data is not required.
Z-stack image
Z-stack image
High-speed extraction
Best focus
CLICK
Z-stack image
High-speed extraction
Best focus
Completion of
best-focus,
time-lapse image
Moving image
generation
High-speed extraction
Time-lapse image can be
converted into video file (AVI)
format with a click on the
[Moving Image] button.
Best focus
Time
Time-series Brightness Measurement Function
This function measures a change in RGB brightness level of time-lapse images in chronological order, to create graphs and text files. Up to
8 measuring points are available. Polygon, circle and free-form curve can be selected by area designation.
R
G
B
Measurement
graph
Measuring function
Measured image
Memo
Time-lapse shooting time inserting function
Shooting time and time interval data embedded
in the image can be displayed.
Histogram
Maintaining cell activity Installable temperature and CO2 concentration control chambex
The temperature and CO2 concentration control chamber can be installed on the main body. This provides long-time time-lapse shooting
of live cells.
Chamber
You can observe cells
in the darkroom status
with the front panel closed.
Temperature CO2 control chamber (971817) *Picture on the left
Temperature CO2 control chamber with mixing device (971948) *Picture on the right
19
Real-Time 3D Module BZ-H1R
(Optional)
Advanced function that enables accurate observation of local
expression, to report observation results to a third party.
The BZ-9000 can accurately recognise an expression point in a cell or nucleus by creating an image on XYZ 3D coordinates.
Operation screen
Hela Cell
Real-time Adjustment
Her2 3D image
Separates fluorescence signals for R, G and B after synthesis
of a 3D image, enabling count check.
Moving Image Setting
1. Volume rendering:
A method to visualize the brightness levels of the points (voxels) in a 3D
space by converting them directly into a 2D image, instead of converting
them into polygons. This results in a significant amount of data and
calculations because not only the surface information but also the internal
information is reflected.
2. OpenGL
An OS-independent library used for high-quality real-time 3D graphics. It
can be used to directly operate the hardware functions of a video card
(rendering engine).
After synthesis of a 3D image, it can be saved as a 3D
moving image by setting an angle step.
Real-time 3D Analysis
3D rotation
Zoom
Section (X-Y, Y-Z, or X-Z direction)
Drag with the left mouse button
Mouse wheel
Drag with the right mouse button
XY image
XZ image
20
YZ image
Various Z-stack processing
XYZ slicing function
Max projection
A cross-sectional image can be
obtained at the desired
coordinates of X-Y, Y-Z, or XZ.
You can check the location of
Fluorescent-coloured materials in
the crosssectional structure.
You can see the maximum
brightness in the X-Y, Y-Z, or X-Z
direction. This function is useful
for example to check the overall
image of a cell.
You can observe the cross-sectional view
at a desired point by dragging these lines.
Measuring Module BZ-H1M
(Optional)
Enables easy quantification of observation results
Dimension Measurement
Winding line length
Area
Line profile
Measure the length of a cell’circumference
or nerves.
Measure the amount of expression in a
specified area.
Brightness level by the colour between two
points or on a free-form curve is displayed
relative to pixel coordinates.
Histogram
2 points
Perpendicular line length
Measure the distance between the two
points specified on the screen.
Measure the length of a perpendicular line
drawn to the specified reference line.
Radius
Distance between parallel lines
Measure the radius of the circle determined
by the three points specified on the screen.
Measure the distance between a specified
reference line and a line parallel to the reference
line (the shortest distance to the reference lines).
2 centres
Broken line length
Measure the distance between the centres
of the two circles specified on the screen.
Measure the length from the start to
the end of a broken line.
Count
Winding line length
Count the number of points specified on
the image.
Measure the length from the start to the
end of a winding line.
Angle 1
Angle 2
Measure the angle determined by a vertex
and two points specified on the screen.
Measure the angle determined by two lines
specified on the screen.
Polygon area
Curve
Measure the area and brightness of a
polygon.
Measures brightness level on a free-form
curve.
The brightness distribution of an area is
displayed as a histogram.
Circle area
Measure the area and brightness of a
circle.
Image Processing
Hue/Chroma/Lightness adjustment
Black balance
Hue and chroma can be changed without a change in
fluorescence signal gradation.
Adjusts background of a fluorescence observation image.
Before hue correction
After hue correction
Before
black balance correction
Other Functions
❙ Filter
❙ Image calculation/integration/averaging
After
black balance correction
Symbol/Comment function
❙ Gray conversion
❙ Negative-positive reversing
❙ Black balance
❙ White balance
Scale, comment, arrow, date and time
can be inserted into an image.
21
SPECIFICATIONS OF THE FLUORESCENT FILTER SETS
Shift control filter
Set name
Unit: nm
Excitation Absorption Dichroic mirror
wavelength wavelength
wavelength
Normal filter
Unit: nm
Excitation Absorption Dichroic mirror
wavelength wavelength
wavelength
Set name
OP79304 SB filter DAPI-BP
377/50
447/60
409
OP66834 BZ filter DAPI-BP
360/40
460/50
400
OP79301 SB filter GFP-BP
472.5/30
520/35
495
OP66835 BZ filter GFP
480/30
510-
505
OP79303 SB filter TRITC
543/22
593/40
562
OP66836 BZ filter GFP-BP
470/40
535/50
495
OP79302 SB filter TexasRed
562/40
624/40
593
OP66837 BZ filter TRITC
540/25
605/55
565
OP79305 SB filter Cy5
628/40
692/40
660
OP66838 BZ filter TexasRed
560/40
630/60
595
OP66839 BZ filter Cy5
620/60
700/75
660
FLUORESCENT FILTER SPECTRUM DATA
Shift control filter spectrum data
OP79304 SB filter DAPI-BP
OP79301 SB filter GFP-BP
OP79302 SB filter TexasRed
OP79305 SB filter Cy5
OP79303 SB filter TRITC
Standard filter spectrum data
22
OP66834 BZ filter DAPI-BP
OP66835 BZ filter GFP
OP66836 BZ filter GFP-BP
OP66837 BZ filter TRITC
OP66838 BZ filter TexasRed
OP66839 BZ filter Cy5
SPECIFICATIONS
Model
BZ-9000 (HS All-in-one fluorescence microscope)
Basic optical system
Objective lens
Nikon CF160 Series infinite optical system
Six-mount electronic revolver
Two-mount manual switching
Fixed image-forming lens, electronic LC filter insertion/removal unit
Electronic zoom lens: 0.5x to 3x
Objective lens switching
Image-formation optical system
Microscope unit
Bright-field, Fluorescence, Phase-contrast (Ph1 only)
Bright-field, Fluorescence, Phase-contrast (Ph1, Ph2)
Observation mode
40 x 40 mm stroke, 1 µm pitch min.
Electronic XY stage
Electronic Z stage
7 mm stroke, 0.1 µm pitch min.
8 mm stroke, 0.1 µm pitch min.
Up to four filters can be mounted. Automatic position recognition and automatic excitation shutdown during filter replacement
Electronic filter turret
Fluorescent incident illumination
Fluorescence dimmer mechanism
Rod fibre optical system
Electronic field aperture (synchronised with zoom)
Electronic dimming (5%, 10%, 20%, 40%, 100%)
Manual slot x 1 (Diffusion)
Manual slot x 2
Dimmer (10%, 20%, 40%), Diffusion
Operating distance: 30 mm, Pop-up mechanism (with automatic lamp off function)
Transmitted illumination optical system
Transmitted illumination mechanism
Electronic switching, Electronic bright-field aperture (100% max.)/Phase-contrast slit (Ph1/Ph2)
Halogen lamp
120 W, Average life: approx. 2000 hours
Black space
The stage is fully contained in a built-in darkroom.
Image pick-up element
2/3 inch, 1.5 million pixel monochrome CCD (colourised with LC filter)
Output signal, gradations
Frame rate
8-bit R/G/B
15 frames/second for 8-bit monochrome recording (up to 100 frames/
second with binning) 7.5 frames/second for 12-bit monochrome or color recording
7.5 frames/second
ROI (partial scan)
On-chip binning (2 × 2, 4 × 4, 8 × 8)
Soft binning (2 × 2, 3 × 3)
Enabled
N/A
Peltier cooling: 5°C (at ambient temperature of –25°C)
CCD cooling mechanism
4,080 × 3,072 max (12.5 million pixels, high-quality interpolation)
Number of pixels in recorded image
15 fps max. (8-bit monochrome, 1360 x 1024);
30 fps (with binning, 680 x 512); 60 fps (336 x 248); 100 fps (168 x 120);
7.5 fps (12-bit monochrome, 1360 x 1024)
Moving image shooting
7.5 fps (1,360 × 1,024)
Auto; 1/12,000. to 60. (144 increments)
Electronic shutter
Auto: 1/10000 to 60 sec. (137 increments)
Arbitrary area, average/peak
Metering method
0 dB, +6 dB, +12 dB, +18 dB, +24 dB
Gain
ISO sensitivity: 200, 400, 800, 1600
Push-set, manual
White balance
Push-set, manual, preset
Push-set, manual
Black balance
Electronic revolver control driver software
N/A
Electronic revolver control driver software using control mouse
Electronic XY stage control driver software using control mouse
Electronic stage control driver software
Electronic turret/
zoom control driver software
Software
2/3 inch, 1.5 million pixel color CCD
12-bit monochrome, 8-bit monochrome, 8-bit R/G/B
Binning
Electronic turret/optical zoom control driver software
using control mouse
N/A
Moving image recording software
N/A
Enabled
4-channel image quick switching/capturing software
Multi-colour image capturing software
Multi-colour & Z-stack image capturing software
Z-stack image capturing software
Multi-point image capturing software
Joint image capturing software
Quick full-focusing software
Software for capturing multi-colour Z-stack image while moving Z-axis stage
N/A
Software for capturing Z-stack image while moving stage to Z-axis
Software for capturing image while moving electronic XY stage to preset XY coordinates
N/A
Software for synthesising and jointing adjacent images in XY direction
N/A
Software for synthesising full-focus image while moving electronic Z stage
N/A
N/A
Software for displaying scale on preview screen
Scale display software
Windows XP*1 with SP2
Applicable OS
PC interface
IEEE1394a
+15 to +40°C
+15 to +35°C
Ambient temperature
Controller
Manual slider, Bright-field aperture (85% max.)/Phase-contrast slit (Ph1)
12 V, 100 W, Average life: 1000 hours
Ultra-high-pressure mercury lamp
Camera unit
BZ-8100 (All-in-one Fluorescence Microscope)
Inverted type fluorescence phase-contrast microscope
Relative humidity
35 to 80% RH (no condensation)
493 (H) × 345 (W) × 592 (D) mm*2
Dimensions
410 (H) × 312 (W) × 557 (D) mm
34 kg (without objective lenses mounted)
Weight
Power supply
28 kg
100 to 240 VAC, 50/60 Hz
400VA
Power consumption
390VA
Overvoltage category
Pollution degree
2
*1 Windows XP is a registered trademark of Microsoft Corporation in the U.S.A.
*2 With the front panel closed.
DIMENSIONS
When panel is opened
677.2
486
345
7
592
646.5
23
SPECIFICATIONS OF THE FLUORESCENT FILTER SETS
Extensive lens lineup that can respond to any kind of specimen observations
CFI Plan Apo 2x (971826)
NA0.1 WD8.5 Prepared slide/petri dish
CFCFI Plan Apo 4x (971836)
NA0.2 WD20 Prepared slide/petri dish
CFI Plan Apo 10x (971821)
NA0.45 WD4.0 Prepared slide
CFI Plan Apo 20x(971804)
NA0.75 WD1.00 Prepared slide
CFI Plan Apo 40x(971844)
NA0.95 WD0.14 Prepared slide
CFI Plan Apo VC60xH(971805)
NA1.40 WD0.13 Prepared slide Oil immersion
CFI Plan Apo VC100xH(971891)
NA1.40 WD0.13 Prepared slide Oil immersion
S PI FIELWD ADM 20xC(971962)
NA0.45 WD8.2 to6.9 Prepared slide/petri dish
S PI FIELWD ADM 40xC(971963)
NA0.60 WD3.6 to2.8 Prepared slide/petri dish
OPTIONS
BZ Blank filter cube (OP-66840)
Temperature CO2 control chamber
(971817) *Picture on the left
Temperature CO2 control chamber
with mixing device (971948)
*Picture on the right
BZ multi plate (OP-74800)
(Large plate for multiwell use)
BZ stage holder (OP-74801)
(Slide fixing holder)
❙ BZ desktop PC (971856)
❙ Immersion oil NF50CC (971806)
❙ BZ mercury lamp (OP-85674)
❙ Dimmer filter MF4 (OP-78905)
■ ND filter (OP-85673)
■ 21-inch monitor (971872)
■ 17-inch monitor (971882)
Related Product
All-in-one Type Fluorescence Microscope
BZ-8000 Series
❙ Space saving design eliminates the need for a darkroom
❙ Advanced blur elimination
❙ Image Merge function
Specifications are subject to change without notice.
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Copyright (c) 2009 KEYENCE CORPORATION. All rights reserved. BZ9-WW-C-E 0109-1 600727 Printed in Japan
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