1
Sl.No1. Specification for Water Purification System
1. General: Compact, Wall mountable, sleek water purification system for Ultra –
Pure Type-I water for molecular biology application.
2. Quality of water:- Should deliver Type I/Ultra –pure as per International
specifications as follows: Resistively: 18.2 MΩcm; TOC (typical):< 5ppb;
Bacteria:<1CFU/10ml;
Endotoxin:
<0.001
EU/ml;
RNase:
20pg/ml;
DNase:0.002ng/ml
Important : Should have an ability to deliver Type III water also
3. Volume: 10-12 litre/day.
4. Feed –water: Potable tap water
5. General Essential Features:
(i)
Preferably should start giving pure water immediately after start.
(ii) Should have built in RO, Dual Wavelength UV lamp, Purification pack
or DI cartridge.
(iii) Should have built-in integral tank with minimum 5-7 litre capacity.
(iv) Facility to attach external point of use Bio filter or any such filter to
control endotoxin.
6. Dispenser: Should have a flexible handle dispenser, preferably, with a facility
of :
a. Variable flow drop by drop up to 2 litre per minute.
b. Locked dispense for glassware filling.
c. Auto volume dispense from 50ml to 60 liters.
7. Display: TOC TEMPERATURE, conductivity, volume dispensing, alarm and tank
water level indicator.
8. Other important features:Sanitization facility to avoid contamination
(i)
Facility of intermittent water recirculation to avoid contamination
build-up.
(ii)
USB Port to capture data.
9. Accessories:(i)
UPS/Stabilizer/CVT as required for functioning of the equipment
(ii) All cartridges, filters, pump or any such item which is /are essential
for Installation and functioning /operating the equipment.
10. Consumable: Must Quote separately for consumables (cartridges, filters etc.)
for ONE YEAR for trouble free working.
11. Warranty: For TWO years guarantee plus warranty for at least three years.
12. List of User: Must include a list of user of the same model of the equipment
along with valid email ID and phone no and other contact details. In case the
list is NOT provided or incomplete, the quotation will NOT be considered for
technical/price comparison.
2
Sl. No. 2
Refrigerated micro centrifuge
Technical Specifications:
•
•
It should be table top model with ergonomic design.
Must have wide temperature range of -4°C- to + 40°C, which is modifiable during the
run operation.
•
•
Centrifuge must have a fast cool function where.
Must have a standby cooling and heating mode up to at least 6 hours which enables cooling
or heating even when not in operation.
Must have automatic temperature start up at a specific time to automatically pre-prepare
temperature before centrifugation
Must be able to maintain at 4°C at max. speed for the entire period of opeartion
Max speed of approximately 17,500rpm, max rcf of 30,000g adjustable from 100rpm
upwards in an increment of 10rpm up to 3000rpm and in an increment of 100rpm
thereafter.
Acceleration time to maximum speed must be less than 15s for all rotors at max load and a
deceleration time of less than 15s with the standard 30 x 1.5/2ml rotor, but should have an
option to accelerate and decelerate gently for sensitive samples.
Timer for run can be set up to 99minutes continuous.
It should not produce sound at maximum speed with noise level less than 60 dB.
It should be provided with fixed angle rotor accommodating 30x 1.5/2ml tubes, 6x 15/50ml
and 8 x 8-PCR strips.
•
•
•
•
•
•
•
•
•
It should come with 3 years warranty and on-site repair services as and when
required.
Technical features described above should be supported by original illustrated
catalog, with list of users (with all contact detail), and authentication of vendor’s
business record in the form of Income Tax return for, at least, of last 3 years
3
Sl. No. 3.
Automated Peptide Synthesizer (01 Unit)
1. A fully automated and programmable solid phase peptide synthesizer with all
essential accessories listed at Sl.No. 20 below
2. All operations of solid phase peptide synthesis during the synthesis cycle must
be performed with single machine unit
3. The synthesizer must have provisions for performing chemical synthesis of
peptides using either FMOC or BOC chemistry without any hardware
modification in the system
4. System must have provision for synthesis of at least three peptides
simultaneously with completely different peptide sequences in each reaction
vessel using three independent programmes
5. Variable scale of synthesis : From 50 micro mol to 0.30 milli mol or more
6. Reaction vessels compatible with different synthesis scales must be provided
essentially with the machine. A spare set of reaction vessels (compatible for
different synthesis scale) must be provided essentially with the system
7. At least 36 amino acid reservoirs must be provided to store amino acid stock
solutions and these reservoirs must be connected to reaction vessels with
pressurized tubings under inert atmosphere of nitrogen gas
8. The peptide synthesis vessels should be made up of inert, solvent resistant and
corrosion resistant material like teflon or glass
9. The reaction vessels must be connected with inert pressurized tubings to deliver
solvents and amino acids as well as to drain excess reactants and washings
under vacuum
10. The machine should essentially have software controlled, at least two separate
solvent delivery robotic arms: one for delivering the solvents and reactants to
reaction vessel and the other to deliver piperidine exclusively during deprotection
step
11. Essentially there should be provision for mixing the reactants in the reaction
vessel by either 180o rotation or vortex mechanism. The equipments for enabling
this mixing mechanism like compatible air-compressor with dryer or vortexer
must be provided essentially with the machine
12. The system should automatically take care of complete activation of amino acid
derivatives by mixing the reactants under inert nitrogen atmosphere and transfer
of desired amino acid into each reaction vessel as per set programme
13. Atleast five solvent reservoirs (made of solvent resistant material) should be
available for storing different solvents like DMF/DCM, Methanol/Isopropanol,
Diisopropyl ethylamine, Piperidine and activating reagents
14. Waste containers of at least 20L capacity with Teflon inserts should be provided
with the machine
15. The machine must allow the user to interrupt synthesis reaction at any desired
time to take out a sample of resin from synthesis vessels for checking coupling
efficiency and to test the reaction conditions
4
16. Security and mal functioning alarms must be inbuilt with machine and should be
displayed in the event of low solvents, amino acids and other reagents in the
reservoirs, complete filling of waste container, low inert gas pressure, presence
of air bubbles in the delivery tubings, power failure etc.
17. Entire procedure of peptide synthesis should be controlled by a computer
equipped with user friendly software with flexibility to custom design different
synthesis protocols as per needs of the user
18. The software may have provision to allow the user to change the synthesis steps
at any desired time during synthesis. There should be provision to re-start the
synthesis protocol from any desired step during synthesis cycle
19. The bidder must essentially agree to perform on-site chemical synthesis of at
least two peptides of about 20 to 25 mer length (as per users requirement)
satisfactorily at the time of installation of machine in the lab
20. The system must be essentially provided with the following items:
a. A dedicated computer with latest configuration to operate the machine and
installing operating software of machine
b. One branded color laser printer for taking the printouts of the reaction
parameters and synthesis data for documentation purpose
c. One online 5KVA UPS with at least one hour power backup should be
provided essentially for ensuring uninterrupted machine operation during
synthesis in the event of power failure
d. Three filled nitrogen cylinders with regulators for continuous supply of AR
grade nitrogen gas to maintain the inert environment during pressurized
delivery of solvents and reactants
e. One self-standing fume hood (4 feet width) with provisions for corrosion
resistant interiors, front sliding glass door, inert gas port, vacuum port, water
tap and electricity socket should be provided essentially for handling the
aerosol producing and hazardous solvents used during peptide synthesis.
The ducting for connecting the fume hood to exterior of lab should be
provided by the bidder
f. One split Air Conditioner (1.5/2.0 Tonne capacity) should be provided
essentially with the system to maintain the ambient working temperature for
the automated peptide synthesizer
g. The consumable chemicals and reagents for peptide synthesis must be
essentially with the system in quantities listed as Rink Amide Resin (100GM),
Wang Resin (100GM), All twenty FMOC-L-Amino Acid Derivatives (100GM
each), All twenty FMOC-D-Amino Acid Derivatives (25GM each), Coupling
Reagents HBTU (500GM), HOBT (500GM) and TFA (1KG)]
h. Along with system, all hardware consumables compatible with the machine
like tubings, filters, reagent reservoir vials, reaction vials, septa and
membrane caps etc. should be provided in sufficient quantities to enable
uninterrupted peptide synthesis
5
i.
The cost of all consumables like chemicals, reagents and machine
hardware items (listed at Sl. Nos. g and h above) must be quoted
separately against each items
21. Warranty on the machine and its essential accessories for a period of at least two
years from the date of installation must be provided and AMC charges for
additional three years must be given separately
6
Sl. No. 4
Inverted fluorescent microscope with image taking capability supported by
software and computer
Technical specifications
Body of the microscope should be with Infinity optical corrected optical system,
Extendable optical free space upto 80mm for attaching others attachment in
future, facility for 2 way (100:0, 20/80 left port) or more light distribution of light,
up/ down focusing, side port for attaching digital camera upgradable to one
additional port for attaching another camera, binocular tube with built-in Bertrand
lens & darkslide shutter along with diopter adjustment facility.
• Should have Universal turret condenser (suitable for all microscopy techniques)
with 5 position
• It should have 12V 100W Pre-centred Halogen Illumination system
• It should be fitted with Eyepiece: 10X with F.O.V 22 or better and diopter
adjustment facility on both eyes, anti fungus type
• Microscope should have sextuple revolving nosepiece to accommodate six
objectives at a time.
• Must be supported with DIC Attachment with polarizer & Analyzer with
respective prism
• XY Rectangular mechanical stage should be also in the microscope
• High performance Long working distance Objectives suitable for Brightfield
/Phase Contrast Observation with facility of cover-glass correction. 4X, 10, 20x,
& 40X
• Specification for Epi fluorescence: With 6 position turret filter block, Noise
Terminator mechanism incorporated for high signal to noise ratio images with100W
HG Illumination for Epi-fluorescence. Band pass Fluorescent filters for FITC/GFP
(Ex 465-495, DM 505, BA515-555), TRITC/Rhoda mine/PI/Alexa Fluor (Ex 540/25,
DM 565, BA605/55), DAPI/Hoechst (Ex 340-380, DM 400, BA435-485)
applications so that no cross talk is allowed.
• Should be attached with a Digital Cooled camera with Specifications:- Digital
Cooled camera capable of handling brightfield, fluorescence, DIC, darkfield images
with 2/3” high density ccd chip, approx. 5.1 million pixel resolution. Peltier Cooling
20 deg below ambient Live display mode: (5m interlace mode – 5.9 frames/sec.; 23
frame per / sec. With roi & binning); binning modes: 2x2, 4x4, digital zoom: up to
16x (8 steps); interval shooting : 5 sec. – 12 hr intervals. Software should come along
with camera for acquiring & capturing of images of reputed make. Should have
separate modes for different microscopy techniques i.e., Brightfield, fluorescence,
dic, darkfield images. Fire Wire port for attaching camera onto desktop/laptop
through single wire.
•
Software should be with following features:
•
Acquisition and device control upto three-dimensional acquisition, Image Acquisition,
Time Lapse Imaging, Z-Stack, Annotation, ND Viewer, Large Image, Macro, Report
7
•
Generator facility, Manual Measurement, Vector layer & Multi-Dimensional File Format
(ND format)
Microscope, camera & software should be from same manufacturer for better
compatibility.
•
Data Processing Unit: Branded Desktop PC (with a latest processor at least Core
I3, TFT monitor at least 19” size, 3 GB RAM and 320 GB hard disk, and
preloaded with latest operating system software) as accessories and Color Laser
Printer of reputed make.
•
Microscope should have the provision to be upgraded for motorization.
•
Technical features described above should be supported by original illustrated
catalog, with list of users (with all contact detail), and authentication of vendor’s
business record in the form of Income Tax return for, at least, of last 3 years
Following Accessories that must be supplied with the equipment:
1) On-line 5 KVA UPS with a minimum of 30 minutes back-up
For data back-up one additional system in the form of a laptop PC from a reputed make
that has at specifications such as Intel core I5 processor, 3GB RAM, 320 GB hard disk,
screen size more than 15” with windows7 and MS office 2010. This computer should
also be supported by data analysis software of real-time PCR experiments
8
Sl. No. 5
Specifications for Real Time PCR
Technical Specifications:
Should be a fast gradient real time PCR system for Nucleic acid and protein analysis
Should have thermal block to accommodate 96 well PCR plate, 0.2ml strips or 0.2ml individual
tubes of standard format
System should support fast PCR protocol with ramp rates of ~ 4°C/sec for heating and 3°C/sec
for cooling
Block temperature should be maintained at 20°C till the start of PCR cycle to minimize nonspecific amplification
System should have Optical Detection Module with minimal mechanical movement to capture
sensitive fluorescence emission from reaction volumes as low as 5ul – 100ul
System should have a minimum of 6 peltier elements beneath the block to maintain block
homogeneity for improved data reproducibility
System should have the flexibility to adjust ramp rates to run standard protocol without
change in hardware.
Should have temperature Gradient range of 20°C in all three steps of PCR to provide
additional flexibility while extrapolating established PCR protocols to suit real time chemistries.
Excitation Light Source (LED Array) with a life time warranty with the capability to excite
commercially available dyes that have emission range max of 520-550 (like SYBR Green, Cy5, Cy3,
HEX, FAM, VIC and JOE etc).
System should have at least 2 emission filters and channel photomultiplier as detector to
provide a 9 log dynamic range
Should have particle protection technology to prevent dust accumulation in the optics for
reliable fluorescence detection
Software should support performing
o Absolute quantification,
o Relative quantification,
o Melting curve analysis,
o Allelic Discrimination,
o Plus/Minus assays,
o End point assays
o Thermofluor assay for Protein Characterization.
Software should provide raw data of fluorescence as well as advanced options to export
configurations compatible with analysis software like qBASE, geNorm, REST and GenEx
GenEx and Beacon Designer original version software to be supplied along with the
instrument.
It should be possible to use the system for regular PCR applications by switching off the optical
module
It should have Open system to accommodate consumables and reagents from different
commercial firms.
System should be calibrated / validated and should be traceable to international standards
System should be able to support twin-tec, white and transparent plates for ease of their
handling for better results.
Vendor should have a fully functional Application laboratory and technical support team to
support and trouble shoot problems.
9
The instrument should be supplied with 1 Desktop having following configuration: Processor
(Intel Core i3-370M), 3GB RAM, 320 GB HDD and at least 19’’ inch screen. It should also be
supplied with heavy duty laser printer of reputed make.
RS 232 communication port and printer interface
It should be supplied with three years Warranty and on demand repair services as and when
required.
Technical features described above should be supported by original illustrated catalog, with
list of users (with all contact detail), and authentication of vendor’s business record in the form of
Income Tax return for, at least, of last 3 years
Following Accessories that must be supplied with the equipment:
1) On-line 5 KVA UPS with a minimum of 30 minutes back-up
2) Split AC (1.5 tons) of reputed make only
3) For data back-up one additional system in the form of a desktop from a reputed make
having following configuration: Processor (at least Intel Core i3-370M), 3GB RAM, 320 GB
HDD and at least 19’’ inch screen.
10
Sl. No. 6
Biosensor System (01 Unit)
1. Fully automated Biosensor System working on the principle of Surface Plasmon
Resonance (SPR) for real time, label-free monitoring of bio-molecular
interactions
2. The system must be capable to carry out real-time data acquisition and analysis
of the acquired data
3. The system, software and analysis chemistry must facilitate multi-sample
analysis. It must be possible to vary the use from a single sample run to
unattended multi-sample runs over a period of time.
4. The system must select samples automatically from vials or microplates for
analysis.
5. The system must allow analysis of samples at minimum two analysis spot
positions overlaid on a single sensor surface.
6. The system must have possibility of auto-recovery of samples for further
analytical investigations
7. The system must have an inbuilt auto-sampler for programmable recovery of
samples in multi-well format or vials
8. System must have provision to deliver the solvents or buffers on the sensor
surface using at least two separate pumps or dispensers or fluidic channels
9. Reservoirs made of inert material for storage of chemicals and buffers used in
bio-molecular interaction analysis and waste container must be provided
10. The sensor surfaces must be compatible with different chemistries used to
immobilize the bio-molecules like Amine coupling, Thiol Coupling, Aldehyde
coupling, Streptavidin-Biotin interactions hydrophobic interactions etc.
11. The machine must be supplied with a starter kit to demonstrate the real-time biomolecular interactions during installation of machine
12. Analysis Software: Acquisition and evaluation of data through user-friendly,
computer operated comprehensive analysis software. The software must have
flexibility for developing the desired methods as per requirements. All operations
like flow rate, sample volume, auto recovery of samples, analysis time and
analysis temperature etc. must be software controlled. Malfunctioning alarms
must be displayed for better performance of the machine. There must be option
to change sequence of operations using simple and user-friendly commands.
The analysis software must have following provisions :
a. To calculate concentration of active bio-molecules in solution
b. To determine affinity constants for molecular interaction
c. To determine kinetic rate constants
d. To determine binding affinities
e. To determine thermodynamic parameters
f. To store and export data files in user-friendly format for documentation
13. Sensor Surfaces: The sensor chips compatible with machine with details as
given below must be supplied essentially with the biosensor system mentioning
cost of each items separately
11
a. Gold coated sensor chips (Plain)
x 2)
b. Gold coated CM Dextran sensor chips amino modified
c. Gold coated CM Dextran sensor chips carboxyl modified
d. Protein A immobilized sensor chips
: 100 Nos (50
: 10 Nos
: 10 Nos
: 10 Nos
14. Technical Information
Measuring Principle
Type of
information/Data
Presentation
Sample type
Sample handling
Sample recovery
Sample capacity
Sample volume Range
Injection volume
Sample recovery
Analysis time per
sample
Detection spots per
sensor surface
Surface Plasmon Resonance (SPR)
Real time, Label-free monitoring of molecular Interactions
Concentration of analytes
Kinetics and affinity constants
Thermodynamic Parameters
Biomolecules like Protein, DNA, RNA, antigen, antibody
and Organic molecules like peptides, crude cell extracts,
lipid vesicles, drugs and viruses etc.
Automated sample loading and injection in desired
quantities
Automatic sample recovery in 96 vials or 96/384 well
plates
Facility to store a minimum of 96 samples/reactants in
vials or plates
In Microlitres
20ul to ≥120ul
Fully automatic recovery of samples
1minute to <10 minutes
Atleast two spots per sensor surface for detecting
interaction between immobilized molecules and test
sample against a reference
Number of channels
At least two channels for delivering samples on the
sensor surface
Sensor Surface
Gold coated glass/solid surface
Surface Chemistries
Sensor surface must be compatible with chemistries
commonly used for site directed immobilization of biomolecules
Re-use of sensor
The sensor surface must withstand regeneration buffer
surfaces
pH and conditions for repeated use
Refractive Index Range 1.33 to ≥1.37
Analysis temperature
4°C to ≥ 40°C
Molecular weight
200 Daltons or more
detection
Baseline noise
~ < 0.5 RU/min
Electric voltage
220-240
15. System must be essentially supplied with a dedicated computer with latest
configuration for operating the biosensor system and installing operating and
analysis software of machine
12
16. System must be essentially supplied with one branded color laser printer for
taking the printouts of biomolecular interaction data for documentation purpose
17. System must be essentially supplied with branded 5KVA Online UPS with at
least 1 hour power backup for ensuring uninterrupted functioning of biosensor
system
18. One split Air Conditioner (1.5/2.0 Tonne capacity) should be provided essentially
with the system to maintain the ambient working temperature for the biosensor
system
19. Warranty on the machine and its essential accessories for a period of at least two
years from the date of installation must be provided and AMC charges for
additional three years must be given separately
20. Letter of consent for providing all software updates/additions and supplying the
sensor chips in future for a period of at least five years from the date of
installation, as per requirement of the user must be provided essentially.
21. All available sensor surfaces/chips and other accessories compatible with
the system like sensor surface preparation unit etc. must also be coated as
optional mentioning the price separately against each item
13
Sl. No. 7.
Bioanalyzer (01 Unit)
1. Micro-chip based automated system for DNA, RNA, protein and preferably cell
analysis
2. The system should be capable of measuring size, integrity and concentration of
nucleic acids (DNA and RNA) and proteins
3. The system must provide an automated process of sample delivery, sample
injection, electrophoretic separation, staining and de-staining, data analysis and
display
4. Simultaneous analysis of multiple samples
5. Highest assay accuracy and precision
6. Capacity to analyze low sample volumes (1 to 5 µl) and multiple sample loading
7. Temperature control during electrophoresis
8. Fluorescence detection
9. Separate kit for bio-molecules. With all required accessories and
DNA/RNA/protein/ cell analysis kits. The system should include kits for analysis of
at least 100 samples.
10. Works across a wide range of genetic applications
11. Software for one point instrument control, operation and data analysis
12. Various data display option and output
13. Power 220-240V
14. With software and computer system. To be supplied with branded desktop
computer with latest configuration along with one color laser printer
15. To be essentially supplied with one branded online UPS with 30 minute power
backup
16. The price of all consumables required for running the machine should also be
quoted separately
17. Machine should be covered with at least two year warranty
14
Sl. No. 8 Cell culture hood (Biosafety level 2.0)
Technical specifications
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Biosafety cabinet should be class II, type A2
Nominal Size should be at least 1.2 meters ( 4 Feet)
Internal and external dimension (WxDxH in mm) should be larger than 1200x600x650
mm and 1375x800x1550, respectively
Efficiency should be ≥ 99.99% inhibiting particles ≥0.3 µm to provide around 70% down
flow air and 30% exhaust
Average Airflow Velocity Inflow and down flow should be at least 0.50 m/s (>100 fpm)
and 0.35 m/s (70 fpm), respectively.
Should be vibration free & low noise working station (<65dB).
Should be Fully compliant tested and certified U.S. Standard NSF 49 and European
standard EN 12469, UL listed, SEFA member
Cabinet should have Fluorescent Lamp ,Germicidal UV lamp with automatic control
Cabinet Construction should be of corrosion proof stain resistant stainless steel interior
with antimicrobial powder (for example epoxy) coated body offering product, operator
and environment protection
Should have easy-to-use microprocessor controlled system with alarm.
Bio safety Cabinet should have Ergonomic pitched profile and can be decontaminated
with common antimicrobials.
Installation, testing, validation, demonstration/technical presentation should be
conducted.
Thick laminated safety glass sash door counter balance should be there.
Bio safety Cabinet must have internally electrical socket outlet, universal service fittings
for gas, water, air and vacuum.
Differential pressure gauge must be there.
Should Workable on power supply of 220-240V, 50/60Hz.
It should be provided with support stand, castor wheels, fixed foot or foot masters.
Should Furnish all the documents like NSF 49 certificate, EN 12469 certificate etc. in the
form of hard copy.
Technical features described above should be supported by original illustrated catalog,
with list of users (with all contact detail), and authentication of vendor’s business record
in the form of Income Tax return for, at least, of last 3 years
Following Accessories that must be supplied with the equipment:
I) Filtration system with Vacuum pump
o
o
o
A compatible vacuum pump fitted to an additional filtration unit from any reputed
make. This is to be used for aspiration of spent media and also filtering the fresh
media. Vacuum pump should have the following specification
Die-cast aluminum made body, with Neoprene Diaphragm, Stainless steel Seals
Polyurethane Filter Elements
Weight should be less than 7.0 kg with occupying minimum space (Upto 8" length
x11" height x 7" width)
Should be capable of operation in 210-240 V: 50-60 Hz
15
o
o
o
o
o
o
Should be able to generate a vacuum of at least 60 cm Hg with a pressure of 60 or
more psig
The vacuum should be operated by a motor of 1/8 hp or more capacity
Must be able to supply and maintain Oil-free, dust-free delivery system in the
laboratory
Filtration unit with all accessories like funnel body, stem, Lid support screen and
vent plugs should be made up of internally standard materials for using in Biosafety
cabinet to filter the media. Should be provided with variable sizes of funnels (150500 ml) with effective corresponding diameters for each funnel, Stem should fit to
into standard one-hole stopper, entire system should be able to withstand multiple
autoclaves, should be able to hold 47mm or better filter sizes.
Vacuum Pump and Filtration Unit should be supplied with a compatible Vacuum
Moisture Trap container that could trap aspirated /used media or spent tissue
culture liquid materials
The entire system should be compatible with the Bio safety cabinet.
II) This equipment should be accompanied with one window AC (1.5 tons) for providing
controlled environment to ensure proper functioning of the equipment.
16
9. Mini Thermal Cycler
Specification
•
•
•
•
•
•
•
•
Capacity-can accommodate 25-48, 0.2ml tubes
Ramping speed: 2.5°C/Sec or above
Temperature range: 0-99°C
Heated lid
Alphanumeric representation of protocols
Wide range of user-programmable preferences
Display: LCD display
1KV online UPS
2 years warranty
10. Analytical Balance
Specification
•
•
•
•
•
•
•
•
•
Maximum Capacity -220g
Readability-0.01mg
Repeatability-0.015 to 0.03mg
Linearity -0.1mg
Taring range -0 to 220g
Sensitivity offset -2x10-6 Rnt
Setting Time – about 2s
Sensitivity Accuracy-2.0x10^-6.Rnt
Sensitivity Stability -1x10^-6/a.Rnt
17
S. L. No. 11
SPECIFICATIONS INCUBATOR SHAKER With Refrigeration
A. General: Bench top microprocessor controlled Refrigerated Incubator Shaker
B. Temperature Range:
(i) Temperature control range from 7°C above ambient to 60°C, control accuracy
±0.1°C.
(ii) Should be able to maintain 37°C during summer months when ambient
temperature is 40-45°C
C. Shaking features:
i. Electronically controlled shaking mechanism provides quiet operation. Brushless
motor is preferable to avoid costly maintenance and noise-less
ii. Type of shaking: Orbital. But having any additional feature is advantageous.
iii. Speed: 30 – 300 rpm with control accuracy of ±2 rpm
iv. It should have acceleration/deceleration circuit prevent sudden starts/stops to
eliminate flask closure wetting which would otherwise interfere with oxygen transfer.
v. It should have timer of up to 99.9 Hrs.
D. Display:
(i) Large easy to read display screen clearly indicates speed, running time, alarm
condition and temperature character
(ii) Display is clearly visible from all angles across the room, and in the dark.
E. Safety Features:
i. It should have audible and visible alarms alert for over-temperature, over-load,
over-speed and leakage
ii.There should be over temperature protection
iii. Preferably there should be a option to mute the audible alarms
iv. The system should have RS-232 port for Data Logging.
v. The system should have facility to calibrate the temperature and speed via
keypad.
vi. Electronic and mechanical components should be enclosed, protected from
accidental spills.
vii. It should have automatic restart after power interruption with non-volatile
memory.
viii. It should have open lid cut-out switch stop agitation when chamber is open.
F. Essential Accessories:
(i) It should come with Universal Platform having capacity to holds assortment of
various size of flask sizes upto 2-5 Ltrs and test tube racks. System should be supplied
18
with 250ml clamps (5 Nos.), 500ml clamps (05 Nos), 1000ml (02 Nos) and 2000ml (0102 Nos)
(ii) It should be supplied with compatible stabilizer/ servo for smooth operation
G: Essential Certification and User List:
(i) It should meet CSA, UL & CE standards. Certificates, clearly indicating the make,
model and name of the equipment are to be enclosed.
(ii) The quote should have the List of the user of the quoted equipment/model (with
valid phone number, email ID) of
H. Warranty:
(i) Should have Two year standard warranty from the date of installation
(ii) Should quote for the price of CMC/AMC per year after the expiry of standard
warranty
19
Sl. NO. 12
SPECIFICATIONS INCUBATOR SHAKER
A. General: Bench top microprocessor controlled Refrigerated Incubator Shaker
B. Temperature Range:
(i) Temperature control range from 7°C above ambient to 60°C, control accuracy
±0.1°C.
(ii) Should be able to maintain 37°C during summer months when ambient
temperature is 40-45°C
C. Shaking features:
vi. Electronically controlled shaking mechanism provides quiet operation. Brushless
motor is preferable to avoid costly maintenance and noise-less
vii. Type of shaking: Orbital. But having any additional feature is advantageous.
viii. Speed: 30 – 300 rpm with control accuracy of ±2 rpm
ix. It should have acceleration/deceleration circuit prevent sudden starts/stops to
eliminate flask closure wetting which would otherwise interfere with oxygen transfer.
x. It should have timer of up to 99.9 Hrs.
D. Display:
(i) Large easy to read display screen clearly indicates speed, running time, alarm
condition and temperature character
(ii) Display is clearly visible from all angles across the room, and in the dark.
E. Safety Features:
ix. It should have audible and visible alarms alert for over-temperature, over-load,
over-speed and leakage
x.There should be over temperature protection
xi. Preferably there should be a option to mute the audible alarms
xii. The system should have RS-232 port for Data Logging.
xiii. The system should have facility to calibrate the temperature and speed via
keypad.
xiv. Electronic and mechanical components should be enclosed, protected from
accidental spills.
xv. It should have automatic restart after power interruption with non-volatile
memory.
xvi. It should have open lid cut-out switch stop agitation when chamber is open.
20
F. Essential Accessories:
(i) It should come with Universal Platform having capacity to holds assortment of
various size of flask sizes upto 2-5 Ltrs and test tube racks. System should be supplied
with 250ml clamps (5 Nos.), 500ml clamps (05 Nos), 1000ml (02 Nos) and 2000ml (0102 Nos)
(ii) It should be supplied with compatible stabilizer/ servo for smooth operation
G: Essential Certification and User List:
(i) It should meet CSA, UL & CE standards. Certificates, clearly indicating the make,
model and name of the equipment are to be enclosed.
(ii) The quote should have the List of the user of the quoted equipment/model (with
valid phone number, email ID) of
H. Warranty:
(i) Should have Two year standard warranty from the date of installation
(ii) Should quote for the price of CMC/AMC per year after the expiry of standard
warranty
21
13.
Liquid nitrogen Cell storage system
Technical specifications:
•
•
•
•
•
•
•
•
•
•
In system Storage type should be manual fill in rack and box.
Capacity should be at least 60 liters with more than 8 inch neck diameter.
Static holding time should be more than 80 days and static evaporation time must
be less than 0.9 liter/day
System should contain at least four racks for holding of 2 ml vial boxes, each box
should provide place for 100 vials.
System capacity for storage should be 2000 or more vials each of 1.2/2.0 ml
capacity.
System exterior should be not more than: - 22.0 x 27.0 inch (Diameter x Height)
System should preferably be fitted with liquid nitrogen level monitor, suitable for
connecting to a power supply of 240V.
System should have 3 year warranty from the date of installation.
System should come wheeled accessory cart for easy movement of the storage
system
Technical features described above should be supported by original illustrated
catalog, with list of users (with all contact detail), and authentication of vendor’s
business record in the form of Income Tax return for, at least, of last 3 years.
22
S.L NO. 14
Specification for Electroporator
1. Electroporator system with the capability to electroporate eukaryotic cells,
prokaryotic cells (bacteria), yeasts and other micro-organisms.
2. Instrument should have features for adjusting voltage & pulse length
3. Pulse voltage rang:10-3,000V
4. Should include all accessories for electroporation.
5. It should also have required software, pre- set optimized programmes and other
accessories.
23
S.L. NO 15
SPECIFICATION DETAILS OF CO2 incubator
1.
TYPE: Moderate sized (table top with smaller foot-print), microprocessor
controlled multi-sided heating with provision for accurate and precise controls for
CO2 and relative humidity. Suitable for Mammalian Cell Culture
2.
CAPACITY: App. 120-200 litre or more
3.
TEMPERATURE RANGE AND UNIFORMITY: ± 2-3 °C ambient temp to 50°C
or better; With control of + 0.1°C, Uniformity of + 0.1°C. Should have the external
or internal features/ arrangement so that the machine can maintain the 37°C during
summer months when ambient temperature is 40-45 °C
4.
REGULATION RANGE CO2 range: 0.2 to 20%, with control range + 0.1%;
uniformity ± 0.1%; Accurate CO2 sensing using IR or better sensing technology;
Auto- start Function
5.
HEATING SYSTEM: Muti-sided heating Fan-less convection Should have
maximum thermal protection and quick (≤ 4-6 minutes) recovery from swings in
ambient temp and door opening.
6.
CO2 SENSING Infrared (IR) or better
7.
INLET FILTER FOR CO2 HEPA filter: <0.3 micron at 99.999% for optimum
filtering, easy access, and simple replacement (no tools needed).
8.
DECONTAMINATION: Auto sterilization facility at high temperature of 121°C or
using better/improved method
9.
10.
DISPLAY & SAFETY :Large display to show set and achieved set parameters of
Temp, CO2, RH, Clock, graphical option and preferably with history
programmable alarms, diagnostics
INTERIOR:
1. Sealed, joint less, Fan-less and polished stainless steel interior with 100% coved
corners is easy to clean, saving time and reducing contamination risk.
2. Condensation free interior to avoid growth of contaminants during culture
11.
SHELVES: Removable and adjustable stainless shelves
24
12
ESSENTIAL ACCESSORIES:
1. Should be supplied with standard accessories, and all the prerequisites for
installation and functioning of the equipment like door lock, air conditioning,
calibration kit, filters, Cylinder auto-changer, and alarm system etc
2. Should be supplied with compatible branded online UPS/ Stabilizer with
minimum half an hour backup having INTERNAL dry SMF battery which should
have warranty/AMC as same as for the incubator
3. Should be supplied with one cylinders and one regulator and all other accessories
13
OTHER MANDATORY FEATURES:
1. MUST have International ISO, CE, UL certified (certificate must be enclosed).
2. Firm MUST provide a compliance statement vis-à-vis specifications in a “tabular
form in the prescribed format” clearly stating the compliance and giving
justification, if any supported by technical literature
3. A list of users of the model quoted in the present Tender only along with valid
email ID and contact numbers and a certificate that they have no objection in case
the users are contacted
14
ON-SITE WARRANTY: Two years standard comprehensive warranty and three
years AMC with detailed terms & conditions so that equipment is covered at least
for the entire project period. Rates may be shown separately.
25
Sl. No. 16
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Specifications for Fluorescent Microscope with camera
attachment
Should have a basic stand with LED illumination, four nosepiece and HCS (harmonic
component system) Optics, with 20 mm field of view
Should have automatic adjustment of illumination to the contrast methods, auto-off
function, LED with service life of 50,000 hours, constant colour temperature
Should have bright field, phase contrast, integrated modulation contrast upgradeable to
fluorescence
Should have trinocular tube with beam splitting position Vis/Phot: 100/0,50/50 & 0/100
Should have four position nosepiece
Should have Objective 4×, 10×, 20× FWD 3.7mm & 40× FWD 2.0mm & 10× eyepiece
Should have condenser suitable for all the above techniques
Should have large stage 248×212×20 mm. (optional-XY adjustment 83×127 mm &
holders
Should have fluorescence filters for Rhodamine, FITC and DAPI with 100 Watt Hg lamp
for fluorescence
Should have fluorescence slider with three positions for filter cubes
Illumination column should be attachable to both rear & front of microscope stand
Should be provided with high resolution cooled digital camera (same make as that of the
microscope) with 5MP, 2/3”CCD sensor, pixel size 3.4µm × 3.4µm, exposure time 1
msec-600 sec, fast live image (24 Frame/sec) with Firewire cable, interface to produce
1:1 image on PC
Should be provided with a branded PC with latest configuration
Should be provided with UPS one kV offline with 30 minutes back up
Should be provided with a branded colour printer for making photographic record
26
Sl. No. 17
Specifications for Bio Safety Cabinet Class II, Type B2 (total
exhaust)
Biosafety cabinet of class II, Type B2 (Total Exhaust) with vertical laminar airflow
complying to EN12469:2000 with microprocessor based monitoring system.
Automatic control of all functions and all safety alarm systems with double
centrifugal fan to provide complete operator, product and environmental
protection. Suitable for handling pathogens namely Mycrobacterium
paratuberculosis.
Internal dimension (WxDxH) should be approximately 850X 700X 550 mm or
more
The cabinet should be fitted with 1 automatic safety service connection for gas, 1
for vacuum and 1 electrical socket
Dual, long life ULPA/HEPA (H-14 grade, according to EN1822) filters for supply
and exhaust airflow. The cabinet should consist of ducting facility on the top of
the cabinet for direct ducting to facility exhaust system.
The Cabinet should be supplied with a UV sterilizing lamp
Frameless, shatter-proof sash with automatic UV shut-off on sash opening
Machine should have low noise level ( below < 53 dB)
The cabinet should have user friendly practical keyboard and display to inform
laminar airflow and frontal air barrier velocity , residual lifetime of HEPA filter , UV
lamp , total number of hours of operation, saturation level of HEPA filter, inside
and outside Temperature
Audio and visual alarms required for power failure, out of range or incorrect
laminar airflow velocity and frontal air barrier velocity, end of life-cycle of UV lamp
, fan-motor malfunction ,saturation of HEPA filters , un-correct position of front
sash-window, blockage in the exhaust duct.
Interior work area of a single piece of stainless steel and single piece HIGH
GRADE stainless steel. Work surface should be consisted of sections easily
removable for carrying out routine cleaning and/or require autoclaving
sterilization procedures if so desired.
Cabinet should be preferably coated with anti bacterial treatment/ solution to
prevent microbial contamination.
Cabinet should also be supplied with modular stand with castors.
Air flow velocity should be at least 90 fpm; efficiency should be > 99.99% at 0.1
micron to 0.3 micron to provide 100% exhaust.
Safety device: (i) Dual-wall construction surrounds the work zone with negative
pressure plenums for maximum safety. (ii) Fail-safe system ensures that in case
of exhaust failure
Must meet American (NSF/ANSI) or European standard EN 12469 (type tested)
or both. Must submit a copy of EN 12469 or NSF/ANSI certification along with the
quote (it is mandatory and without the valid certificate the quote will be
considered as non-responsive).
27
Sl.No18. Water purification system
Technical Specifications:
•
The system should be capable to handle potable tap water conditions.
•
It should support the following applications:
Ist stage system for laboratory applications
The unit should have the following purification stages:
Activated carbon for the removal of free chlorine & organics.
Reverse osmosis stage for removal of ionic & inorganic impurities.
Conductivity cell should be present before RO stage to measure the RO feed
conductivity & system should be capable of measuring percentage of rejection
for correct monitoring.
Advanced self regenerating electro-deionisation module resistant to scaling
should be used.
Water quality: Flow rate should be at least 3 L/hr, Ions organics removal upto
99%, TOC < 50 ppb or less.
•
Additionally, for Molecular Biology applications, the Unit should have a IInd stage
system to provide ultrapure water with following features:
Resistivity: 18.2 MOhm.cm, Conductivity: 0.055uS/cm, Bacteria: < 0.1cfu/ml,
Flow rate: upto 1 liters/min, TOC: < 10 ppb, Endotoxin< 0.001 Eu/ml.
Automatic recirculation feature.
•
It should have a Reservoir Tank of at least 50 liter capacity
•
Technical features described above should be supported by original illustrated catalog,
with list of users (with all contact detail), and authentication of vendor’s business record
in the form of Income Tax return for, at least, of last 3 years
Following Accessories that must be supplied with the equipment:
1) The equipment should be accompanied with a 2 KV online UPS to back the power supply
for at least 30 minutes.
2) 1 portable water purifier for other supporting and general lab work and consumable for
media filtration filters.
28
Sl. No. 19 Geldoc for both transillumination and chemiluminescence imaging system
with photographic capabilities attached to a CCD camera and computer with software
The equipment is expected to be used for applications such as, 2-D protein gels including
multiplexing, Stain Free gel and blot imaging, 1-D nucleic acid and protein gels, Proteomics
and Genomics studies, Western blots – Chemiluminescence and 3-color multiplex
fluorescence and also for Northern and Southern blots studies
Technical Specifications required for this equipment is as follow:
•
•
•
•
•
•
•
•
•
•
•
•
•
•
System should be with true 16 bit CCD (0-65,535 levels), with > 4 Megapixel image
resolution
Cooled CCD with cooling range of -30°C absolute temperature using peltier cooling
system (Not air cooled)
System with dynamic flat fielding technology for getting accurate Quantitation with
CV < 5 %.
Multi image color target with multi-channel image display for study of more than 2
dyes at a time for differential expression. Automatic sequential imaging of multi
labeled samples.
Instrument should have a large transillumination area which is capable of taking
sample of minimum 28x36 cm and should have a minimum imaging area of 26 x 35
cm.
Must support capability of stain free gel imaging to avoid time consuming
gel/sample staining procedure.
Instrument should have auto focus technology that is system should automatically
take the best focus depending on any zoom level without the movement of sample
platform. The sample platform should be fixed at one position to ensure minimal
mechanical movement.
Pixel size should be 6.45 µm x 6.45 µm or bigger.
Instrument should have a minimum 6 position filter wheel for capturing images of
various dyes effectively. The filter wheel should be motorized and automatic
without the requirement of manually changing or moving the filter wheel.
Dynamic range should be >4 orders of magnitude for good quantification.
Pixel density should be at least 65,535.
System should have at least 8 different modes of illumination like, Trans-UV, epiwhite, no illumination for chemiluminescence, trans-white, epi-red, epi-green, epiblue, and optional trans-blue. All LEDs should be integrated with system for proper
imaging of multi target with reproducibility.
System should offer various binning options with minimum of 2x2, 3x3 and 4x4 for
customized sensitivity.
Data Processing Unit: desktop PC (with a latest processor at least Core I3, TFT
monitor at least 19” size, 3 GB RAM and 320 GB hard disk, and preloaded with
latest operating system software)
29
Software for Image acquisition and Analysis:
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Should support automatic generation of customizable reports with data organized
as desired, including, Lane and band identification, molecular weight or base pair
evaluation. Band sizing and quantification should be based on a reference band or
quantity standards.
Snapshot tool to copy images, lane profiles, and graphs
Complete flexibility with automatic and manual detection of lanes and bands,
molecular weight determination, purity assessment, and relative and absolute
quantification using several algorithms.
Should have tri-plex image capture capability and analysis features.
Easy copy/paste functionality, crop, zoom, 3D viewer, and colors.
Should provide functionality to easily produce image at user-defined dpi and
dimension to support publication
Should have multiple image optimization tools like unlimited undo and redo
functions to easily correct for any missteps
Original software should be provided free of cost, and with up-gradation without
any extra cost in future. No requirement for license registration
software should be Mac and PC compatible
16-bit and 8-bit tiff images with a one-click export option
Software should automatically select the appropriate filters, light sources, and
camera settings for all applications. It should also ensure that image optimization is
specific to a selected gel or blot application or any specific portfolio of detection
methods. Applications should support: Chemiluminescent, colorimetric and
multiplex fluorescent western blots, nucleic acid and protein detection via
colorimetric and fluorescent stains.
Software should have automated multi-channel image view and color-coded
analysis.
Software should have Signal Accumulation Mode (SAM) for easy optimization of
exposure time for chemiluminescent detection.
Software should have ability for unlimited installations of the software for multiple
users with full functionality.
Software should offer live update of results with any change of analysis parameters.
Accessories:
I. Horizontal Mini/Midi gel electrophoresis system
•
•
•
•
•
Complete system with all accessories for running Agarose gels.
Should be able to cast gels of size 7 x 7 cm and 7 x 10cm.
Should have the sample throughput of 8 – 30 .
UV Transparent trays with an integrated fluorescent rulers.
Gel caster with Leak proof casting facility, without using tapes. Also option for
casting dams should be there.
30
Should come with 8, 12, and 15 wells fixed height comb
Should have replaceable electrodes for longer life of equipment.
Suitable Power pack to support the system
•
•
•
II. Vertical Electrophoresis SDS Page System with Blotting apparatus:
Mini Electrophoresis system with 7x8 cm gel size with capacity of 2-4 gels
simultaneously ( Preferably 4 gels)
Should have integrated spacers with glass plates for ease of casting.
Should be a modular system to support blotting and electro-elutions also in the
same system.
Should come all standard accessories like 10 well, 0.75mm comb, 5 sets of glass
plates –both spacer plates, and short plates.
Should come with sample loading guide.
•
•
•
•
•
Blotting Apparatus should have the following technical features
•
•
•
•
•
•
Gel size 10 x 7.5 cm or any standard specification
Buffer requirements:- >400 ml
Capacity:- 2 mini protean hand cast gels, 2 mini precast gels
Placement of electrode should be 4 cm or more apart for strong electric fields
and efficient protein transfer.
Color coded cassettes and electrodes to ensure proper orientation
Blue cooling unit that absorbs heat generated during rapid transfer
Power Supply: for Running and transfer of large gels
•
•
•
•
•
•
Programmable power supply should be capable to operate four electrophoresis
units simultaneously for four identical runs with graphic 3 digit LED display.
The output range should be up to 10- 300 V , 4- 400 mA in I m A steps, 75 W
Constant voltage, or constant current Horizontal Electrophoresis system
Timer – 1- 999 min
No load detection, sudden load change detection, short circuit detection, over
voltage protection.
Technical features described above should be supported by original illustrated
catalog, with list of users (with all contact detail), and authentication of vendor’s
business record in the form of Income Tax return for, at least, of last 3 years
31
Following Accessories that must be supplied with the equipment:
1) To support controlled environment one Split AC (1.5 ton) from any reputed
make should be provided
2) An additional data back-up system in the format of a laptop PC from a reputed
make with a latest processor (at least Core I3) and 3 GB RAM and 320 GB hard
disk, screen size more than 15” and preloaded with latest operating system
software and 4 lab work stools should be given free of cost.
32
S.L. NO. 20
Automated Blood Gas analyzer for Veterinary Use
Specifications
1.
2.
3.
4.
5.
Features to measure pH, pCO2, pO2, measure Oximetery parameters, Electrolytes
Test Samples: Blood, Plasma, Serum in small amount
Analyzer controller with soft ware for analysis of multiple parameters
LCD screen with Printer
Facility for regular quality assessment of the instrument should be provided by
the company
6. Warranty : At least 3 years
Provide reagents with the analyzer to run the machine satisfactorily for two years
(@1000 samples/year).
33
Sl. No. 21 Blood Flow Meter
(To measure quantitative skin blood flow rate in the micro circulatory beds of skin and
other tissues of livestock under heat stress, suitable under Indian conditions)
Specification:
Mode: Continuous laser Doppler flowmetry
Primary Measurement: Microvascular blood perfusion (Relative RBC Flux)
LDF Units: Relative units (0–7500 Blood Perfusion Units, corresponding to 0–7.5 V
output)
BSC Units: Relative units (0–100% corresponding to 0–7.5 V output)
Laser Type: Semiconductor laser diode (temperature stabilized)
Laser Wavelength: 830 nm +10 nm
Laser Power: < 0.5 mW from probe
Laser Class: Class 1 (as per 21 CFR 1040-10 and 1040-11)
Doppler Signal Bandwidth: 16 kHz
Linearity Range: up to 0.35% moving scatterers by volume.
Flow Response Time: < 0.2 s
Reading Stability: 5% (measured with standard motility solution)
Zeroing: Automatic, controlled
Flux Calibration: Factory or user calibrated using a motility standard (concentration of
latex spheres undergoing Brownian Motion)
Power Supply
Voltage Range: 100–240 V AC (50–60 Hz), 0.8–0.4 A
Output Power: +5 V, }12 V DC
Fusing: Internal, 2 A 240 V resettable
Physical Configuration
Power Requirements: 240 V or 120 V (internally set) @ l5 W
Operating Temperature: 0–50 °C
Operating Humidity: 0–100% (non-condensing
34
Sl. No. 22
Specifications for Real Time PCR
Real time PCR with block of 96 x 0.2 ml tubes or plate to run typical 0.2ml tubes, strips,
and plates
The base thermal cycler should be able to be used for standard PCR
Gradient capacity in Real-time
Should have a maximum ramping speed of 5 OC per sec
Should have the capacity to detect FIVE different fluorescent reporters in the same
tube.
Should be capable of detecting Cy5, FAM/Sybr Green, VIC/JOE, TAMRA/Cy3, Texas Red,
Quasar705
Should have six excitation and six emission channels each filter corresponding to one
dye
that ensures smooth differentiation of even dyes having high degree of spectral overlap
Should have peltier cooling & heating
Should have one channel dedicated for FRET experiments
Should have excitation –emission range of 450- 730nm
No internal reference dye should be required. True 5 Color Multiplexing with use of 5
different flourophores without the need of addition of any internal reference dye
Should have LED excitation source with Photodiode detector
Should have dynamic range of 10 orders
Should be an open system capable of running various chemistries like TaqMan,
Molecular Beacon, SYBR green
Should have temperature range of 0– 100 OC with accuracy of ±0.2 OC and uniformity of
±0.4 OC within 10 sec of arrival at 90 OC
Should be able to run with a minimum sample volume of 1µl
Should be able to detect ≤ 10 fmol of fluorescein
Should have multiple scan modes with a FAST scan option for reading all wells in 3
seconds
Should have facility for automatic allelic discrimination by end point fluorescence or
threshold cycle
Gene expression analysis by relative quantity (∆Ct) or normalized expression (∆∆Ct)
Should have end point analysis for upto 5 fluorophores
Should have mode for Melt curve analysis
Comparison of upto 5000 Ct values from different data files should be possible
Should be supplied with 2KVA online UPS to provide uninterrupted power supply,
branded PC/laptop for analysis and cooling system to maintained ambient temperature
Analysis of multiple experiments from a single plate should be possible
Warranty of 1 year and CMC free of cost for the second year