Deutsche Akkreditierungsstelle GmbH
Annex to the Accreditation Certificate D-PL-14085-01-00
according to DIN EN ISO/IEC 17025:2005
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
Holder of certificate:
MQD Qualitätsprüfungs- und Dienstleistungsgesellschaft
Mecklenburg-Vorpommern mbH
Speicherstrasse 11, 18273 Güstrow
Tests in the fields:
physical, physico-chemical, chemical, microbiological, molecular-biological, immunological and
sensorial analyses of foods, particularly milk and dairy products and beverages;
microbiological and molecular-biological analyses of animal feeding stuffs;
microbiological analyses for hygiene monitoring in the food sector;
molecular-biological analyses of genetically modifications and genetically modified organisms in
foods and animal feeding stuffs;
microbiological analyses in accordance with the German drinking water ordinance;
sampling of untreated and drinking water as well as of milk and milk products and other foods;
veterinary medicine
test areas:
microbiology, molecular biology, serology
test methods:
culturally analyses
amplification methods
ligand assays
Abbreviations used: see last page
This document is a translation. The definitive version is the original German annex to the accreditation certificate.
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Within the given testing field marked by * the testing laboratory is permitted, without being
required to inform and obtain prior approval from DAkkS,
*)
**)
***)
the free choice of standard or equivalent testing methods.
the modification, development and refinement of testing methods.
to use standard testing methods listed here with different issue dates.
The listed testing methods are exemplary. The testing laboratory maintains a current list of all
testing methods within the flexible scope of accreditation.
1
Foods
1.1
Sampling of foods ***
MQD MET 7327
2014-03
Sampling of foods for sensorial, chemical and microbiological
analyses
ASU L 01.00-43
2010-09
Examination of foods; guidance on sampling of milk and milk
products (DIN EN ISO 707:2009)
ASU L 06.00-59
2011-06
Examination of foods, sampling of carcass for microbiological
analysis (DIN ISO 17604:2011)
1.2
Sensorial analyses
1.2.1
Sensorial analyses of foods using simple descriptive tests *
ASU L 00.90-6
1997-09
Examination of foods; sensorial analyses - simple descriptive
test (DIN 10964:1996)
ASU L 00.90-16
2006-12
Examination of foods; sensorial analyses - expert witness for
the judgement of conformity with foodlaw
(DIN 10975:2005)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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1.2.2
Sensorial analyses of foods using special sensory tests *
DIN EN ISO 22935-2
2012-12
Milk and milk products - sensory analysis Part 2: recommended methods for sensory evaluation
DIN EN ISO 22935-3
2012-12
Milk and milk products - sensory analysis - Part 3: guidance
on a method for evaluation of compliance with product
specifications for sensory properties by scoring
ASU L 01.01/02-1
1988-12
Examination of foods; sensory analysis and assessment of
drinking milk
ASU L 04.00-9
1986-05
Examination of foods; determination of the distribution of
water in butter; indicator-paper method
ASU L 04.00-12
1990-06
Examination of foods; sensory analysis of butter
1.3
Physical, physico-chemical and chemical analyses
1.3.1
Gravimetric analyses of primary ingredients in foods *
ASU L 00.00-18
1997-01
2002-12 (amendment)
Examination of foods; determination of dietary fiber
in foods
ASU L 01.00-9
2012-01
Examination of foods; determination of fat content
in milk - gravimetric method, reference method
(DIN EN ISO 1211:2010)
(modification: drying in a vacuum and active recooling,
reduction of the drying and cooling times)
ASU L 01.00-20
2013-08
Examination of foods; determination of fat content of milk and
milk products using the Weibull-Berntrop gravimetric method
(DIN 10342:1992)
ASU L 01.00-38
2009-06
Examination of foods; determination of fat content in
skimmed milk, whey and buttermilk - gravimetric method,
reference method (DIN EN ISO 7208:2009)
(modification: drying in a vacuum and active recooling,
reduction of the drying and cooling times)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 02.05-2
2009-06
Examination of foods; determination of fat content in
cream - gravimetric method, reference method
(DIN EN ISO 2450:2009)
(modification: drying in a vacuum and active recooling,
reduction of the drying and cooling times)
ASU L 02.06 E(EG) and
1 (EG) bis 8 (EG);
1981-01
Analytical method regarding the composition of certain
partially or completely, dried non-perishable milk products
(modification: method 2)
ASU L 02.06-12
2009-06
Examination of foods, determination of fat content in
evaporated milk and sweetened condensed milk Gravimetric method, Reference method
(modification: drying in a vacuum and active recooling,
reduction of the drying and cooling times)
ASU L 02.07-15
2009-06
Examination of foods; determination of fat content in
dried milk and dried milk products - gravimetric method,
reference method (DIN EN ISO 1736:2009)
(modification: drying in a vacuum and active recooling,
reduction of the drying and cooling times)
ASU L 02.07-20
2006-12
Examination of foods; determination of moisture content in
dried milk - reference method (DIN EN ISO 5537:2004)
ASU L 03.00-8
2007-04
Examination of foods; determination of fat content of
cheese and processed cheese products - gravimetric method
according to Schmidt-Bondzynski-Ratzlaff, reference method
(DIN EN ISO 1735:2005)
(modification: drying in a vacuum and active recooling,
reduction of the drying and cooling times)
ASU L 03.00-9
2007-04
Examination of foods; determination of the total solids of
cheese and processed cheese - reference method
(DIN EN ISO 5534:2004)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 04.00-24/1-3
2013-01
Examination of foods; determination of moisture, non-fat solids
and fat contents of butter
Part 1: Determination of moisture content (reference method)
Part 2: Determination of non-fat solids content (reference
method)
Part 3: Calculation of fat content
ASU L 06.00-3
2014-08
Examination of foods; determination of moisture content in
meat and meat products - gravimetric method, reference
method
ASU L 06.00-6
2014-08
Examination of foods; determination of the total fat content
in meat and meat products - gravimetric method according to
Weibull-Stoldt, reference method
ASU L 13.00-20
2004-12
Examination of foods; determination of unsaponifiable
matter in animal and vegetable fats and oils - method with
diethyl ether extraction (DIN EN ISO 3596:2002)
ASU L 17.00-1
1982-05
2002-12 (amendment)
Determination of the drying loss in bread including rolls of
bread dough
ASU L 17.00-3
1982-05
2002-12 (amendment)
Determination of the ash content in bread including rolls of
bread dough
ASU L 17.00-4
1982-05
2002-12 (amendment)
Determination of the total fat content in bread including
rolls of bread dough
ASU L 44.00-4
1985-12
Examination of foods; determination of the total fat content
of chocolate
VDLUFA MB Bd. VI C 10.2
2000
Determination of the total ash content in milk and milk
products
VDLUFA MB Bd. VI C 15.2.4
1985
Determination of the free fat content in dried milk
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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VDLUFA MB Bd. VI C 15.2.5
2003
Determination of the free fat content of milk and liquid milk
products - gravimetric method
(modification: drying in a vacuum and active recooling,
reduction of the drying and cooling times)
VDLUFA MB Bd. VI C 35.3
1985
Determination of dry matter content of milk and milk products,
sea sand method
DAB 10 V 3.2.14
1991
Determination of sulphated ash in foods
SLMB 1000.1
2009
Determination of the fat content of instant coffee
NIRO-Atomizer
method A8a
2013-06
Determination of particle size distribution in dried milk by
sieving
NiroAtomizer
method A10a
2013-06
Determination of surface free fat of dried milk
1.3.2
Titrimetric analyses
1.3.2.1 Titrimetric analyses of primary ingredients in foods *
ASU L 01.00-10/2
2002-12
Examination of foods; determination of nitrogen content
in milk - Part 2: Block digestion method
(DIN EN ISO 8968-2:2002)
ASU L 06.00-7
2014-08
Examination of foods; determination of crude protein in meat
and meat products - titrimetric method according to Kjeldahl,
reference method
ASU L 17.00-15
2013-08
Examination of foods; determination of crude protein in bread
including rolls of bread dough - Kjeldahl method
VDLUFA MB Bd. VI C 30.4
1985
Determination of casein content of milk
NiroAtomizer
method A1d
2013-06
Determination of moisture content in dried milk products - KarlFischer method
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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1.3.2.2 Titrimetric analyses of secondary ingredients in foods *
ASU L 01.00-7
2002-05
Examination of foods; determination of the level of
acidity in milk and liquid milk products (DIN 10316:2000)
ASU L 13.00-5
2012-01
Examination of foods; determination of acid value and
acidity of animal and vegetable fats and oils
(DIN EN ISO 660:2009)
ASU L 13.00-6
2014-08
Examination of foods; determination of peroxide value in fats
and oils (method according to Wheeler; method according to
Sully)
ASU L 13.00-10
2014-08
Examination of foods; determination of iodine value in
animal and vegetable fats and oils (DIN EN ISO 3961:2013)
ASU L 31.00-3
1997-09
Examination of foods; determination of titratable acidity
of fruit and vegetable juices
(DIN EN 12147:1997)
VDLUFA MB Bd. VI C 8.4
2000
Determination of titratable acidity of dried milk products
VDLUFA MB Bd. VI C 30.3
1985
Determination of non protein nitrogen content (NPN) in milk
and milk products
The BDI method - part I
2000
Determination of free fatty acids in cream and whole milk
powder
1.3.3
Photometric analyses of secondary ingredients in foods *
ASU L 01.00-36
1990-06
Examination of foods; determination of nitrate content
in milk and milk products - xylenol method
ASU L 01.00-41
1991-12
Examination of foods; determination of the phosphatide value
in milk, milk products and cheese
ASU L 03.00-17
1990-12
Examination of foods; determination of the total phosphorus
content of cheese and processed cheese - spectrometric
method (DIN 10324:1990)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 06.00-8
2010-01
2010-09 (amendment)
Examination of foods; determination of hydroxyproline content
in meat and meat products - photometric method using acid
digestion
ASU L 13.00-15
2008-06
Examination of foods; determination of anisidine value in
animal and vegetable fats and oils (DIN EN ISO 6885:2007)
VDLUFA MB Bd. VI C 10.6.6
2003
Determination of nitrite content in milk and milk products,
photometric method
Codex Alimentarius
CAC/GL 13-1991 App. III
1991
Determination of thiocyanate in milk and dried milk products
ADPI Bulletin 946
2009
Determination of undenatured whey protein nitrogen in
nonfat dry milk (WPNI)
(modification: application also for concentrates and other
dried products)
Federal Research Institute
of Nutrition and Food;
Institute for Cereal,
Potato and Starch
Technology
2006-06
Determination of the blue value in potato products method according to Haase
Dairy reports119
1994
Determination of OPA-WLN und OPA-NPN in cheese according
to Tschager
MQD MET 1024
2014-01
Detection of protease in foods according to Stydom
1.3.4
Potentiometric analyses of secondary ingredients in foods *
ASU L 03.00-11
2007-12
Examination of foods; determination of chloride content in
cheese and processed cheese products - potentiometric method
(DIN EN ISO 5943:2007)
ASU L 04.00-13
2006-12
Examination of foods; determination of the pH-value in butter
plasm (DIN 10349:2004)
ASU L 06.00-2
1980-09
Measurement of the pH-value in meat and meat products
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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VDLUFA MB Bd. VI C 8.2
2000
Determination of the pH-value in milk and milk products
VDLUFA MB Bd. VI C 10.6.3
2003
Determination of chloride content in cheese, potentiometric
method
1.3.5
Enzymatic analyses
1.3.5.1 Enzymatic analyses of primary ingredients in foods *
ASU L 01.00-17
2010-09
Examination of foods; determination of lactose and D-galactose
content of milk and milk products - enzymatic method
(DIN 10344:1982)
VDLUFA MB Bd. VI C 20.3
1988
Milk and milk products; enzymatic determination of glucose
and sucrose content of milk products
MQD MET 1008
2012-09
Determination of starch in foods, enzymatic method
MQD MET 1012
2012-09
Determination of sucrose, D-glucose and D-fructose in foods
MQD MET 1019
2012-09
Enzymatic determination of maltose and maltose homologues
in foods
MQD MET 6114
2014-01
Determination of total glucans in food supplements; ß-1,3 - ß1,6-glucans by hydrolysis with trifluoroacetic acid and enzymatic
glucose determination
MQD MET 6207
2014-01
Determination of total glucans in food supplements; ß-1,3 - ß1,6-glucans and mannans by hydrolysis with trifluoroacetic acid
and enzymatic glucose and mannose determination
MQD MET 6277
2014-01
Determination of fructans in foods
1.3.5.2 Enzymatic analyses of secondary ingredients in foods *
DIN 10484
2013-09
Milk; determination of urea and ammonia content photometric method
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 01.00-26/2
2010-09
Examination of foods; determination of D- and L-lactic acid
(L- and D-lactate) content in milk and milk products reflectometric method (screening method)
MQD MET 1007
2014-01
Determination of citric acid content in foods,
enzymatic method
1.3.6
High performance liquid chromatography (HPLC) using standard detectors
1.3.6.1 High performance liquid chromatography of primary ingredients in foods **
ISO 22662
2007-09
Milk and milk products; determination of lactose content by
high-performance liquid chromatography - reference method
ASU L 40.00-7
1999-11
2009-06 (amendment)
Examination of foods; analysis of honey - determination of the
content of saccharides, fructose, glucose, saccharose, turanose
and maltose - HPLC-method (DIN 10758:1997)
MQD MET 6198
2014-03
Determination of rebaudioside A and other steviol glycosides in
sweeteners - HPLC-method
1.3.6.2 High performance liquid chromatography of secondary ingredients in foods **
ISO 13875
2005-02
Heat-treated milk; determination of acid-soluble β-lactoglobulin
content - HPLC-method
(modification: application also for the determination of αlactalbumin and lactoferrin) (IDF 178: 2005)
ASU L 18.00-16
1999-11
Examination of foods; determination of theobromine and
caffeine content in pastries
ASU L 45.00-1
1999-11
Examination of foods; determination of theobromine and
caffeine content in cocoa
ASU L 46.00-3
2013-08
Examination of foods; examination of coffee and coffee
products - determination of the caffeine content using HPLC,
reference method (DIN ISO 20481:2011)
ASU L 47.00-6
2014-02
Examination of foods; examination of tea and instant tea in
solid form - determination of caffeine content - HPLC-method
(DIN 10727:2004)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 47.08-1/1
2002-05
Examination of foods; determination of theobromine and
caffeine content of fluid tea drinks - Part 1: HPLC-routine
method (DIN 10811 Teil 1:2000)
ASU L 47.08-1/2
2002-05
Examination of foods; determination of theobromine and
caffeine content of fluid tea drinks – Part 2: HPLC-routine
method (DIN 10811 Teil 2:1997)
1.3.6.3 High performance liquid chromatography of food additives in foods **
IDF 165
1993-04
Determination of antioxidants in butteroil using HPLC
ASU L 00.00-9
1984-11
Examination of foods; determination of preservatives in
low-fat foods
ASU L 00.00-10
1984-11
Examination of foods; determination of preservatives in
high-fat foods
ASU L 00.00-28
2001-07
Examination of foods; determination of acesulfame-K,
aspartame and saccharin in foods - HPLC-method
(DIN EN 12856:1999)
(modification: application also for the determination of
diketopiperazine)
ASU L 00.00-29
2001-07
2006-12 (amendment)
Examination of foods; determination of cyclamate in foods HPLC-method (DIN EN 12857:1999)
ASU L 00.00-59
2008-12
Examination of foods; determination of isomalt, lactitol,
maltitol, mannitol, sorbitol and xylitol in foods - HPLC-method
(DIN EN 15086:2006)
(modification: application also for the determination of
erythritol)
ASU L 00.00-126
2013-01
Examination of foods; determination of sucralose in foods HPLC-method (DIN EN 16155:2012)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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Merisant-method
2005
Determination of maltose in sweeteners
MQD MET 6308
2014-03
Determination of bronopol in raw milk - HPLC-method
1.3.7
Gas chromatography (GC) using standard detectors
1.3.7.1 Gas chromatography of secondary ingredients in foods *
ASU L 00.00-140/1
2014-08
Examination of foods; determination of cholesterol content in
low cholesterol and starch-free foods - gas chromatographic
method
ASU L 13.00-26
2008-06
Examination of foods; gas chromatographic determination of
methyl esters of fatty acids in animal and vegetable fats and oils
(DIN EN ISO 5508:1995)
ASU L 13.00-27/3
2012-01
Examination of foods; gas chromatography of fatty acid methyl
esters - Part 3: preparation of methyl esters using
trimethylsulfonium hydroxide (TMSH) in animal and vegetable
fats and oils (DIN EN ISO 12966-3:2010)
1.3.7.2 Gas chromatography of contaminants in foods *
ASU L 01.00-35
1990-06
Examination of foods; determination of low-boiling halogenated
hydrocarbons in milk
(modification: detection by GC-MS)
ASU L 13.04-1
2006-12
Examination of foods; determination of low-boiling halogenated
hydrocarbons in edible oils
(modification: detection by GC-MS)
1.3.8
ISO 8070
2007-08
Atomic absorption spectrometry of minerals in foods
Milk and milk products; determination of calcium, sodium,
potassium and magnesium content - Atomic absorption
spectrometric method
(modification: application also for pastries, meat and meat
products, sugar and sugar substitutes, sweets and food
supplements)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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1.3.9
Product-specific physical and physico-chemical analyses in foods
1.3.9.1 Physical-rheological analyses of primary ingredients in foods *
ASU L 04.00-14
1996-02
Examination of foods; determination of the firmness of butter
(DIN 10331:1996)
VDLUFA MB Bd. VI C 26.7
2000
Physical determination of whipping cream
MQD MET 1314
2014-01
Melting- and solidification curve of butter according to Wölbern
1.3.9.2 Analysis of the density of foods using densitometric methods *
ASU L 01.00-28
1988-12
2002-12 (amendment)
Examination of foods; areometrical determination of the density
of milk
VDLUFA MB Bd. VI C 12.3.1
2003
Determination of the density in sour milk products without added
foods
VDLUFA MB Bd. VI C 26.5
1995
Determination of bulk volume and bulk density of dried milk
products
Niro-Atomizer
method A2b
2013-06
Bulk volume and bulk density of dried milk products
1.3.9.3 Refractometrical analyses of primary ingredients in foods *
ASU L 31.00-16
1997-09
Examination of foods; determination of soluble solids content in
fruit and vegetable juices - refractometric method
(modification 1: application also for other foods)
(modification 2: dilutions according withdrawn Standard
ASU L 30.00-2 (EG) 1993-08)
VDLUFA MB Bd. VI C 16.2
1988
Determination of the refraction of milkfat
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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1.3.9.4 Qualitative and semiquantitative physical and physico-chemical analyses of foods
by visual evaluation
Niro-Atomizer
method A6b
2013-06
Dispersibility of dried milk products
ADPI Bulletin 916 revised
1996
Determination of scorched particles in dried milk products
Australian Standard 2300.4.7
1994-01
Determination of the heat stability of milk powder
MQD MET 1172
2014-01
Test of lipase in foods according to Purr
1.3.9.5 Cryoscopic analyses of primary ingredients in foods
MQD MET 1026
2014-01
1.3.10
Cryoscopic determination of the osmolarity of beverages and
beverage bases
Volumetric analyses of primary ingredients in foods *
ASU L 01.00-74/2
2002-12
Examination of foods; butyrometric determination of fat content
of milk and milk products - Part 2: product specific requirements
(DIN 10479-2:2001)
VDLUFA Bd. VI C 15.3.3
1985
Butyrometric determination of fat content of cream according to
Roeder
VDLUFA MB Bd. VI C 15.3.7
1985
Butyrometric determination of fat content of yogurt and
fermented milk (ammoniac method)
VDLUFA MB Bd. VI C 15.3.8
1985
Butyrometric determination of fat content of cheese, method
according to van Gulik
VDLUFA MB Bd. VI C 15.3.9
1988
Determination of fat content of milk powder by Teichert
VDLUFA MB Bd. VI C 26.2
1995
Determination of the solubility of milk powder
(according to ADPI)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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VDLUFA MB Bd. VI C 26.6
2000
1.3.11
Determination of the grade of homogenization of milk
Other analyses
Regulation 1169/2011
Annex XIV (LMIV)
in the current version
Calculation of the energy value of foods
1.4
Immunology
1.4.1
Immunological analyses of contaminants in foods using Enzyme-Linked
Immunosorbent Assay (ELISA) *
ASU L 01.00-34
1989-12
Examination of foods; determination of aflatoxin M1 content in
milk and dried milk products using Enzyme-Linked
Immunosorbent Assay (ELISA) - screening-method
ASU L 01.00-68
1998-09
Examination of foods; determination of chloramphenicol
residues in milk - screening method using ELISA microtiter
system
ASU L 01.00-70
2002-05
Examination of foods; determination of streptomycin- and
dihydrostreptomycin residues in milk - screening method using
ELISA microtiter system
MQD MET 6078
2014-02
Lateral flow assay for detection of beta-lactam-/ cephalosporin
antibiotic residues (penicillin, ampicillin, cephalonium etc.) and
tetracyclin antibiotic residues in milk of cows, goats and sheeps
MQD MET 6229
2014-02
Determination of staphylococcal enterotoxins A, B, C, D and E
in foods, ELISA method
1.4.2
Immunological analyses of allergens in foods using Enzyme-Linked Immunosorbent
Assay (ELISA) *
MQD MET 3901
2014-02
Determination of gliadin / gluten in foods; ELISA method
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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MQD MET 3902
2014-02
Determination of hazelnut in foods; ELISA method
MQD MET 3903
2014-02
Determination of casein in foods; ELISA method
MQD MET 3905
2014-02
Determination of ß-lactoglobulin in foods; ELISA method
MQD MET 3907
2014-02
Determination of peanut in foods; ELISA method
1.5
Microbiological analyses
1.5.1
Sample preparation for bacteriological and mycological cultural analyses of foods *
ASU L 00.00-54
2000-07
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations of foods - Part 1: general rules for the preparation
of the initial suspension and decimal dilutions
(DIN EN ISO 6887-1:1999)
ASU L 00.00-89
2014-02
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations of foods - specific rules for the preparation of
other products than milk and milk products, meat and meat
products, and fish and fishery products
(DIN EN ISO 6887-4:2012)
ASU L 01.00-1
2011-06
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations - Part 5: specific rules for the preparation of milk
and milk products (DIN EN ISO 6887-5:2011)
ASU L 06.00-16
2004-12
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations - specific rules for the preparation of meat and
meat products (DIN EN ISO 6887-2:2004)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 10.00-10
2004-12
1.5.2
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations - specific rules for the preparation of fish and
fishery products (DIN EN ISO 6887-3:2003)
Selective and nonselective cultural bacteriological and mycological analyses
1.5.2.1 Cultural bacteriological and mycological analyses of microorganisms (pour plate
technique, spread plate technique and membrane filtration) in foods **
ISO 8552
2004-05
Milk; enumeration of psychrotrophic microorganisms - colonycount technique at 21 °C (rapid method) (IDF 132: 2004)
ISO 13559
2002-11
Butter, fermented milks and fresh cheese; enumeration of
contaminating microorganisms - colony-count technique at
30 °C (IDF 153: 2002)
ISO 14189
2013-11
Water quality; enumeration of Clostridium perfringens membrane filtration method
(modification: application also for other filterable foods)
ISO 15213
2003-05
Microbiology of food and animal feeding stuffs; horizontal
method for the enumeration of sulfite-reducing bacteria
growing under anaerobic conditions
ISO 15214
1998-08
Microbiology of food and animal feeding stuffs; horizontal
method for the enumeration of mesophilic lactic acid bacteria colony-count technique at 30 °C
ISO/ TS 27265
2009-08
Dried milk; enumeration of the specially thermoresistant spores
of thermophilic bacteria
DIN ISO 21528-2
2009-12
Microbiology of food and animal feeding stuffs; horizontal
method for the detection and enumeration of
Enterobacteriaceae - Part 2: colony-count technique
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 00.00-22
2006-09
Examination of foods; horizontal method for the detection and
enumeration of Listeria monocytogenes - Part 2: enumeration
method (DIN EN ISO 11290-2:2005)
(modification: 9.2.1 - using Brilliance Listeria-Agar; 9.5.1 hemolysis test on Caso-Blood-Agar; 9.5.2 - using api listeria)
ASU L 00.00-33
2006-09
2006-12 (amendment)
Examination of foods; horizontal method for the enumeration
of presumptive Bacillus cereus - colony-count technique at 30 °C
(DIN EN ISO 7932:2004)
(modification: 9.3 - hemolysis test on Caso-Blood-Agar)
ASU L 00.00-55
2012-04
Examination of foods; horizontal method for the enumeration
of coagulase-positive staphylococci (Staphylococcus aureus and
other species) in foods - Part 1: method with Baird-Parker-Agar
(DIN EN ISO 6888-1:2003)
(modification: 9.4 - coagulase test is performed at catalase
positive typical and atypical colonies; 9.5 - the enrichment in
brain-heart broth is dropped)
ASU L 00.00-57
2006-12
Examination of foods; horizontal method for the enumeration
of Clostridium perfringens in foods - colony-count technique
(DIN EN ISO 7937:2004)
ASU L 00.00-88
2004-07
Examination of foods; horizontal method for the enumeration
of microorganisms - Colony-count technique at 30 °C
(DIN EN ISO 4833:2003)
ASU L 00.00-88
2004-07
Examination of foods; horizontal method for the enumeration
of microorganisms - Colony-count technique at 55 °C
(DIN EN ISO 4833:2003)
(modification: 9.2.8 - the incubation temperature is 55 °C)
ASU L 00.00-132/2
2010-09
Examination of foods; horizontal method for the enumeration
of β-glucuronidase-positive Escherichia coli in foods Part 2: colony-count technique using 5-bromo-4-chloro-3indolyl β-D-glucoronide (DIN ISO 16649-2:2009)
ASU L 01.00-3
1987-03
Examination of foods; determination of coliforms in milk, milk
products, butter, cheese and ice cream; method with solid
medium
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 01.00-37
1991-12
Examination of foods; determination of the count of yeasts and
moulds in milk and milk products - reference method
(ISO 6611:2004, ISO 7954:1987)
(modification: application also for other foods)
ASU L 01.00-72
2011-01
Examination of foods; determination of presumptive Bacillus
cereus in milk and milk products - Part 1: colony-count
technique at 37 °C (DIN 10198:2010)
(modification: hemolysis test on Caso-Blood-Agar)
ASU L 42.00-2
1987-03
Examination of foods; determination of the total plate count in
ice cream - pour plate technique
ASU L 59.00-3
1988-05
Examination of foods; detection of Pseudomonas aeruginosa in
natural mineral water, spring water and table water - reference
method
MTVO Annex 2 section 4
2006-12
Determination of sulfite-reducing anaerobic bacteria (clostridia)
in water according to MTVO
(modification: application also for other filterable foods)
VDLUFA MB Bd. VI M 7.3.2
1985-01
Milk and milk products; determination of protein metabolising
microorganisms, method with Calcium-Caseinate-Agar
(for caseolytes)
VDLUFA MB Bd. VI M 7.6.2
1985-01
Milk and milk products; determination of lipolitic
microorganisms (lipolytes) - colony-count technique with
Tributyrinagar
VDLUFA MB Bd. VI M 7.8.2
1993
Milk and milk products; determination of enterococci - colonycount technique with Kanamycin-Aesculin-Azide-Agar
VDLUFA MB Bd. VI M 7.9.2
1988
Milk and milk products; determination of lactobacilli - colonycount technique with ROGOSA-Agar
VDLUFA MB Bd. VI M 7.12.2
1993
Milk and milk products; determination of pseudomonas species
- colony-count technique with C-F-C-Selective-Agar
VDLUFA MB Bd. VI M 7.13
1996
Milk and milk products; determination of thermoduric
(thermoresistant) microorganisms
VDLUFA MB Bd. VI M 7.17.2
1993
Milk and milk products; determination of spores of
aerobic spore formers (Bacillus)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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MQD MET 3372
2014-03
Detection of anaerobic spore formers (Clostridium spp.) in milk
and milk products - method with RCM-Agar
Co-applicable documents
ASU L 00.00-123
2008-12
Examination of foods; general requirements and guidance for
microbiological examinations of foods (DIN EN ISO 7218:2007)
1.5.2.2 Cultural bacteriological and mycological analyses of microorganisms (enrichment
technique, MPN- and Titer technique) in foods **
ISO 4831
2006-08
Examination of foods; horizontal method for the detection and
enumeration of coliforms - MPN-technique
ISO 6785
2001-05
Milk and milk products; detection of Salmonella spp.
(IDF 93: 2001)
(modification: 9.3.2.1 - 1ml of the pre- enrichment is transferred
in 10 ml selective enrichment - selenit-cystine medium)
ISO 21528-1
2004-08
Microbiology of food and animal feeding stuffs; horizontal
method for the detection and enumeration of
Enterobacteriaceae - Part 1: detection and enumeration by
MPN technique with pre-enrichment
(modification: 9.5 - using TSA or Caso-Blood-Agar)
ISO/TS 22964
2006-02
Milk and milk products; detection of Cronobacter spp.
(Enterobacter sakazakii)
DIN EN ISO 7937
2004-11
Microbiology of food and animal feeding stuffs, horizontal
method for the detection of Clostridium perfringens
(modification: application as enrichment method with DRCMBouillon followed by streaking out on SC-Agar)
DIN EN ISO 21871
2006-04
Microbiology of food and animal feeding stuffs - horizontal
method for the determination of low numbers of presumptive
Bacillus cereus - MPN technique and detection method
ASU L 00.00-20
2008-12
Examination of foods; horizontal method for the detection of
Salmonella spp. in foods (DIN EN ISO 6579:2007)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 00.00-32
2006-09
Examination of foods; horizontal method for detection and
enumeration of Listeria monocytogenes - Part 1: detection
method (DIN EN ISO 11290-1:2005)
(modification: 9.4.1 - streaking out only from the 2. selective
enrichment; 9.4 - using Brilliance Listeria-Agar and Oxford-Agar;
9.6.1 - hemolysis test on Caso-Blood-Agar; 9.6.2 - using api
listeria)
ASU L 00.00-90
2004-12
2007-12 (amendment)
Examination of foods; horizontal method for the detection of
presumptive pathogenic Yersinia enterocolitica
(DIN EN ISO 10273:2003)
ASU L 00.00-91
2012-06
Examination of foods; horizontal method for the detection of
Shigella spp. in foods (ISO 21567:2005)
(modification: 9.3.1 – MacConkey-Agar (low-selective) is not
used)
ASU L 00.00-100
2006-12
Examination of foods; horizontal method for the enumeration
of coagulase-positive staphylococci (Staphylococcus aureus and
other species) in foods - detection and MPN technique for low
numbers (DIN EN ISO 6888-3:2005)
(modification: 9.3.1.1 - coagulase test is performed at catalase
positive typical and atypical colonies; 9.3.1.2 - The enrichment in
brain-heart broth is dropped)
ASU L 00.00-107
2007-04
Examination of foods; horizontal method for detection and
enumeration of Campylobacter spp. in foods - detection
method (DIN EN ISO 10272-1:2006)
ASU L 00.00-132/2
2010-09
Examination of foods; horizontal method for the detection of βglucuronidase-positive Escherichia coli in foods - Part 2: colonycount technique using 5-bromo-4-chloro-3-indolyl β-Dglucoronide
(modification: application as enrichment, method with
Laurylsulfat-Tryptose media followed by streaking out on TBXAgar)
ASU L 01.00-2
1991-12
2002-12 (amendment)
Examination of foods; determination of coliforms in milk, milk
products, butter, cheese and ice cream - method with liquid
medium
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ASU L 01.00-54
1992-12
Examination of foods; determination of Escherichia coli in milk
and milk products - fluorescence optical method with parallel
determination of coliforms
VDLUFA MB Bd. VI M 7.18.4
1988
Milk and milk products, determination of sulfite-reducing
clostridia
MQD MET 3361
2014-02
Microbiology of food and animal feeding stuffs; horizontal
method for the detection of Salmonella spp.
Co-applicable documents
ASU L 00.00-123
2008-12
1.6
Examination of foods; general requirements and guidance for
microbiological examinations of foods (DIN EN ISO 7218:2007)
Molecular-biological analyses of microorganisms in foods using Real-time PCR **
ASU L 00.00-98
2007-04
Examination of foods; qualitative detection of salmonella in
foods - Real-time PCR method
ASU L 00.00-122
2008-06
Examination of foods; detection of DNA sequences from
califlower mosaic virus (CaMV 35S-promoter, P35S) and
Agrobacterium tumefaciens (Tnos) in foods often used in
genetically modified organisms (GMO) - screening method
MQD MET 3801
2010-04
Detection of Salmonella in foods using Real-time PCR
"foodproof Salmonella - Biotecon"
MQD MET 3802
2010-04
Detection of Cronobacter spp. and Enterobacteriaceae in foods
using Real-time PCR "foodproof Enterobacteriaceae and E.
sakazakii - Biotecon"
MQD MET 3803
2010-06
Detection of Listeria monocytogenes in foods using Real-time
PCR "foodproof Listeria monocytogenes - Biotecon"
MQD MET 3804 / SOP 37
2014-03
Detection and determination of microorganisms in foods using
Real-time PCR; using method of the test manufacturers
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
- Translation -
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MQD MET 3805
2013-07
Detection of enterohaemorrhagic E. coli (EHEC in foods about
genes for Shiga toxins (stx1 & stx2) and intimin (eae) using Realtime PCR „foodproof STEC Screening LyoKit - Biotecon“
MQD MET 3806
2012-10
Detection of Cronobacter spp. in foods using Real-time PCR,
FDA -PCR Assay (Chen et al., 2012)
MQD MET 3808
2014-03
Detection of Clostridium perfringens in foods using Real-time
PCR "foodproof SL Clostridium perfringens, Biotecon"
MQD MET 3811
2014-03
Detection of naturally occurring califlower mosaic virus (CaMV)
in foods for the verification of genetic modifications using Realtime PCR
2.
Commodity goods
2.1
Enumeration and detection of microorganisms in the environment of foodareas and on
fitment and utensils in foodareas using cultural bacteriological analyses *
DIN 10113-1
1997-07
Determination of surface colony count on fitment and utensils
in foodareas - Part 1: quantitative swab method
DIN 10113-2
1997-07
Determination of surface colony count on fitment and utensils
in foodareas - Part 2: semiquantitative swab method
(modification: 7.3, 7.4 - the inoculation of the nutrient media is
done by direct plating)
DIN 10113-3
1997-07
Determination of surface colony count on fitment and utensils
in foodareas - Part 3: semiquantitative method with culture
media laminated taking up equipment (squeeze method)
ASU B 80.00-5
2011-12
Examination of commodity goods; methods for sampling
techniques for microbiological analysis from surfaces using
contact plates and swabs (DIN ISO 18593:2009)
MQD MET 4101
2014-03
Determination of airborne bacterial count using sedimentation
and impaction method
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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3.
Animal feeding stuffs
3.1
Microbiological analyses
3.1.1
Sample preparation for bacteriological cultural analyses of animal feeding stuffs *
ASU L 00.00-54
2000-07
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations of foods - Part 1: general rules for the preparation
of the initial suspension and decimal dilutions
(DIN EN ISO 6887-1:1999)
(application for animal feeding stuffs)
ASU L 00.00-89
2014-02
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations of foods - specific rules for the preparation of
other products than milk and milk products, meat and meat
products, and fish and fishery products
(DIN EN ISO 6887-4:2012)
(application for animal feeding stuffs)
ASU L 06.00-16
2004-12
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations - specific rules for the preparation of meat and
meat products (DIN EN ISO 6887-2:2004)
(application for animal feeding stuffs)
ASU L 10.00-10
2004-12
Examination of foods; preparation of test samples, initial
suspension and decimal dilutions for microbiological
examinations - specific rules for the preparation of fish and
fishery products (DIN EN ISO 6887-3:2003)
(application for animal feeding stuffs)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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3.1.2
Selective and nonselective bacteriological cultural analyses
3.1.2.1 Bacteriological cultural analyses of microorganisms (pour plate technique, spread plate
technique and membrane filtration) in animal feeding stuffs **
ISO 15213
2003-05
Microbiology of food and animal feeding stuffs; horizontal
method for the enumeration of sulfite-reducing bacteria
growing under anaerobic conditions
ISO 15214
1998-08
Microbiology of food and animal feeding stuffs; horizontal
method for the enumeration of mesophilic lactic acid bacteria colony-count technique at 30 °C
DIN ISO 16649-2
2009-12
Microbiology of food and animal feeding stuffs; horizontal
method for the enumeration of β-glucuronidase-positive
Escherichia coli - Part 2: colony-count technique at 44 °C using
5-bromo-4-chloro-3-indolyl β-D-glucoronide
DIN EN ISO 4833
2003-06
Microbiology of food and animal feeding stuffs; horizontal
method for the enumeration of microorganisms - colony-count
technique at 30 °C
(withdrawn standard)
DIN EN ISO 7937
2004-11
Microbiology of food and animal feeding stuffs; horizontal
method for the enumeration of Clostridium perfringens colony-count technique
DIN ISO 21528-2
2009-12
Microbiology of food and animal feeding stuffs; horizontal
method for the detection and enumeration of
Enterobacteriaceae - Part 2: colony-count method
3.1.2.2 Bacteriological cultural analyses of microorganisms
(enrichment technique, MPN- and Titer technique) in animal feeding stuffs **
ISO 7932
2006-04
Microbiology of food and animal feeding stuffs; horizontal
method for the enumeration of presumptive Bacillus cereus colony-count technique at 30 °C
(modification: 9.4.2 - Hemolysis test on Caso-Blood-Agar)
ISO 21528-1
2004-08
Microbiology of food and animal feeding stuffs; horizontal
method for the detection and enumeration of
Enterobacteriaceae - Part 1: detection and enumeration by
MPN technique with pre-enrichment
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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DIN EN ISO 6579
2007-10
Microbiology of food and animal feeding stuffs; horizontal
method for the detection of Salmonella spp.
MQD MET 3361
2014-02
Microbiology of food and animal feeding stuffs; horizontal
method for the detection of Salmonella spp.
3.2
Molecular-biological analyses of microorganisms in animal feeding stuffs
using Real-time PCR **
ASU L 00.00-98
2007-04
Examination of foods; qualitative detection of salmonella in
foods - Real-Time PCR method
(application for animal feeding stuffs)
ASU L 00.00-122
2008-06
Examination of foods; detection of DNA sequences from
califlower mosaic virus (CaMV 35S-promoter, P35S) and
Agrobacterium tumefaciens (Tnos) in foods often used in
genetically modified organisms (GMO) - screening method
(application for animal feeding stuffs)
MQD MET 3801
2010-04
Detection of Salmonella in foods using Real-time PCR
"foodproof Salmonella - Biotecon"
MQD MET 3811
2014-03
Detection of naturally occurring califlower mosaic virus (CaMV)
in foods for the verification of genetic modifications using Realtime PCR
4.
Analysis according to the German drinking water ordinance 2001 (TrinkwV 2001) ***
Method
DIN EN ISO 5667-01 (A 4)
2007-04
DIN ISO 5667-5 (A 14)
2011-02
DIN EN ISO 5667-3 (A 21)
2013-03
DIN EN ISO 19458 (K 19)
2006-12
Title
Water quality - sampling - Part 1: guidance on the design of
sampling programmes and sampling techniques
Water quality - sampling - Part 5: guidance on sampling of
drinking water from treatment works and piped distribution
systems
Water quality - sampling - Part 3: preservation and handling of
water samples
Water quality - sampling for microbiological analysis
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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ANNEX 1: MICROBIOLOGICAL PARAMETERS
PART I:
Lfd. Nr.
1
2
General requirements for drinking water
Parameter
Escherichia coli (E. coli)
Enterococci
PART II:
Lfd. Nr.
1
2
3
Method
DIN EN ISO 9308-1 (K12) 2014-12
DIN EN ISO 7899-2 (K 15) 2000-11
Requirements for drinking water offered for sale in closed containers
Parameter
Escherichia coli (E. coli)
Enterococci
Pseudomonas aeruginosa
Method
DIN EN ISO 9308-1 (K 12) 2014-12
DIN EN ISO 7899-2 (K 15) 2000-11
DIN EN ISO 16266 (K 11) 2008-05
ANNEX 2: CHEMICAL PARAMETERS
PART I:
Chemical parameters, whose concentration generally do not increase in the drinking
water distribution network including its installation
not performed
PART II:
Chemical parameters, whose concentration may increase in the drinking water
distribution network including its installation
not performed
ANNEX 3: INDICATOR PARAMETERS
PART I:
Lfd. Nr.
1
2
3
4
5
6
7
8
9
General indicator parameters
Parameter
Aluminium
Ammonium
Chloride
Clostridium perfringens
(including spores)
Coliform bacteria
Iron
Colour (spectral absorption
coefficient Hg 436 nm)
Odour
Taste
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
Method
not performed
not performed
not performed
TrinkwV 2001 Anl. 5 I e)
ISO 14189 2013-11
DIN EN ISO 9308-1 (K 12) 2014-12
not performed
not performed
not performed
not performed
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Annex to the accreditation certificate D-PL-14085-01-00
Lfd. Nr.
10
11
12
13
14
15
16
17
18
19
20
21
22
PART II:
Parameter
Colony count 22 °C
Colony count 36 °C
Conductivity
Manganese
Sodium
Total organic carbon (TOC)
Oxidisability
Sulphate
Turbidity
Hydrogen ion concentration
Calcit saturation
Tritium
Total indicative dose
Method
TrinkwV 2001 Anl. 5 I d) bb)
TrinkwV 2001 Anl. 5 I d) bb)
not performed
not performed
not performed
not performed
not performed
not performed
not performed
not performed
not performed
not performed
not performed
Specific requirements for drinking water in facilities of the drinking water installation
Parameter
Method
ISO 11731 1998-05,DIN EN ISO 11731-2 (K22) 2008-06
UBA Empfehlung 2012-08
Legionella spec.
The accreditation does not replace the recognition or approval procedure by the competent
authority according to § 15 passage 4 of the drinking water ordinance (TrinkwV 2001).
5.
Test field: veterinary medicine
5.1.
Test area: microbiology, molecular biology, serology
5.1.1
Test method: cultural analyses
Parameter
Mastitis associated
microorganisms
5.1.2
Matrix
Method
Udder secretion
Cultural analyses
Test method: amplification methods ***
Parameter
Mastitis associated
microorganisms
Mastitis associated
microorganisms
Matrix
Udder secretion
Container milk
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
- Translation -
Method
PCR, PathoProof Mastitis PCR
Assay; Thermo Scientific
PCR, PathoProof Mastitis PCR
Assay; Thermo Scientific
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Annex to the accreditation certificate D-PL-14085-01-00
5.1.3
Test method: ligand assays ***
Parameter
Matrix
Method
Salmonellae antibodies
Pregnancy associated
glycoproteins (PAG)
Meat juice, blood serum
ELISA - SALMOTYPE PigScreen
Raw milk
ELISA - IDEXX
Used abbreviations:
ADPI
ASU
BDI
DAB
DIN
ELISA
IDF
ISO
LFGB
MQD-MET
MTVO
LMIV
PCR
SLMB
TrinkwV
UBA
VDLUFA
American Dairy Products Institute
Official collection of test methods according to §64 LFGB (Amtliche Sammlung von
Untersuchungsverfahren nach § 64 LFGB)
Bureau of Dairy Industries
German Pharmacopoeia (Deutsches Arzneibuch)
German Institute for Standardization (Deutsches Institut für Normung)
Enzyme-Linked-Immunosorbent-Assay
International Dairy Federation
International Organization for Standardization
Food- and Feed Code (Lebensmittel- und Futtermittelgesetzbuch)
In-house method of MQD Qualitätsprüfungs- und Dienstleistungsgesellschaft
Mecklenburg-Vorpommern mbH
Mineral and table water ordinance (Mineral- und Tafelwasser-Verordnung)
Food Information Regulation (Lebensmittelinformations-Verordnung)
Polymerase chain reaction
Swiss Book of Foodstuffs (Schweizerisches Lebensmittelbuch)
Drinking water ordiance 2001 (Trinkwasserverordnung 2001)
Federal Enviroment Agency (Umweltbundesamt)
Association of German Agricultural Analytic and Research Institutes (Verband
Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten)
Period of validity: 10.08.2015 to 09.08.2020
Date of issue: 10.08.2015
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