Curriculum Vitae Dr. Jonas G. Kühn Lerchenrain 19 8046 Zurich SWITZERLAND jonas.kuehn@alumni.epfl.ch Birth date: September 6, 1979 Marital status: Married Nationality: Swiss, French Education 2005–2009 PhD in Science (Photonics) Microvision and Microdiagnostics Group, Advanced Photonics Laboratory, Swiss Federal Institute of Technology, Lausanne (EPFL) PhD dissertation: “Multiple-wavelength digital holographic microscopy” 1998–2003 MSc in Microengineering - Optics Specialization Swiss Federal Institute of Technology (EPFL), Advanced Photonics Laboratory Professional Experience 2015–current Principal Investigator (PI) at Institute for Astronomy, ETH Zurich Three-years “Ambizione” Fellowship from Swiss NSF, including coverage for research costs and personnel. Research on novel instrumental approaches to High-Contrast Direct Imaging of Exoplanets, based on Coherent Differential Imaging (CDI) and Digital Adaptative Coronagraphy. 2014–2015 Caltech Post-Doctoral Scholar at NASA/JPL (60%) High Contrast Imaging for Exoplanets detection by Nulling Interferometry and Coronagraphy in the near-infrared: · Vector Vortex Coronagraph (VVC) on the Subaru 8-m Telescope SCExAO instrument: lead-person. · Stellar Double Coronagraph (SDC) instrument on the Palomar 200-inch Telescope: assembly, commissioning and first light science. · Palomar Fiber Nuller (PFN) instrument on the Palomar 200-inch Telescope: instrument upgrade, calibration and science. (40%) Development of a field-able digital holographic microscope at Caltech, in preparation for a NASA life-dection mission on icy moons (Enceladus, Europa): member of JPL optical engineering team. 1 2012–2014 Post-doc Fellow at Caltech-NASA/Jet Propulsion Laboratory (JPL) Two-years own-funded post-doctoral mobility fellowship granted by Swiss NSF. Research on High-Contrast Imaging for Exoplanets detection by Nulling Interferometry and Coronagraphy in the near-infrared: · Vector Vortex Coronagraph (VVC) on the Palomar 200-inch Telescope (P3K 2nd-gen AO, PHARO imager): engineering and science. · Palomar Fiber Nuller (PFN) instrument on the 200-inch Telescope: assembly, calibration and science. 2009–2012 Post-doc Fellow in the Microvision and Microdiagnostics Group, EPFL Managing digital holography space-related activities: participation at two ESA parabolic flight campaigns, aimed at monitoring muscle cells in micro-gravity. Project manager for several federally-funded projects (SystemsX) to apply digital holographic microscopy to pharmaceutical screening, in collaboration with the EPFL Biomolecular Screening Facility (BSF). 2005–2009 PhD student and teaching assistant in the Advanced Photonics Laboratory, EPFL. Supervision of several student Bachelor and Master projects, as well as EPFL practical lab courses on holography for three consecutive years (2006-2008). 2003–2005 R&D Engineer within the spin-off company Lyncée Tec SA In charge of optical design and laser sources aspects for the first commercially available digital holographic microscopes. Languages French English German Mother tongue Fluent Good Affiliations Senior member of the International Society for Optical Engineering (SPIE). Member and acting as a regular reviewer for the Optical Society of America (OSA). Grants and Awards 2015-2018 Swiss NSF 3-years “Ambizione” PI fellowship and research grant ($600k) 2014 Elected Senior Member of the International Society for Optical Engineering (SPIE) 2012-2014 Swiss NSF 2-years “Advanced postdoc mobility” fellowship ($135k) 2012 “Young Scientist Award” from Society for Laboratory Automation and Screening (SLAS) 2010 “City of Lausanne” Research Award for the PhD Thesis (awarded every 3 years only) 2 Skills Optics Observational astronomy through high-contrast direct imaging and nulling interferometry: planning, observing (40+ nights) and data reduction. Instrumental astronomy, with focal-plane coronagraphs and nulling stellar interferometry: lab assembly, calibration and commissioning at the telescope (3 instruments). Design and built-up of optical systems (for both astronomy and microscopy), with particular skills in related optical alignment (incl. OAPs, fibers, ...). Comprehensive expertise in the field of digital holography, and strong experience in interferometric wavefront metrology in general. Adaptation of optical lab setups to industrial market products or dedicated highconstrains prototypes, including space applications (e.g. instruments compliant with 0-g parabolic flight conditions). Life sciences applications and biomedical imaging, incl. fluorescence microsopy. Academic Author or co-author of several tens of peer-reviewed scientific publications and conference proceedings (see publications list); H-index: 15 (Thomson ISI) Participation at several conferences abroad throughout Europe and in the U.S., including more than a dozen talks Writing of funding requests and proposals, with multiple institutional partners Research project coordinator and management experience (3-5 people) Supervisor of several PhD, Master or Bachelor student projects (see list below), as well as practical lab teaching courses Computing Matlab, IDL, Labview, Mathematica, TEX, MS Office, Adobe Illustrator; Basics in ZEMAX, C and C++ programming Students (co-)advised • M. Fatih Toy, PhD Thesis, EPFL and ETH Zurich (2009-13) • Stéphane Perrin, Master Thesis, EPFL and University of Bourgogne (2010) • Christophe Pache, Master Thesis, EPFL (2007-8) • Mathieu Grossenbacher, Master Thesis, EPFL (2005-6) • Diane Morgan, Semester Project, EPFL (2005) • Sabine Miller, Research Internship, EPFL (2005) 3 Publications list - Current ISI H-index: 15 (as of June 1st, 2015) Refereed papers 1 F. Y. Morales, J. Kühn, D. Mawet, S. Hinkley, B. Mennesson, M. W. Werner, and E. Serabyn, “High-contrast Imaging Survey of Spitzer Two-Belt Debris Disk”, The Astrophysical Journal (in preparation) (2015). 2 M. Bottom, J. Kühn, B. Mennesson, D. Mawet, J. C. Shelton, J. K. Wallace, and E. Serabyn, “Resolving the delta Andromedae spectroscopic binary with Direct Imaging”, The Astrophysical Journal (in revision) (2015) 3 G. Singh, J. Lozi, O. Guyon, P. Baudoz, N. Jovanovic, F. Martinache, T. Kudo, E. Serabyn, and J. Kühn, “On-sky Demonstration of the Lyot-based Low-Order Wavefront Sensor and its integration with the High-Order Pyramid Wavefront Sensor on the Subaru Coronagraphic Extreme Adaptive Optics System”, Publications of the Astronomical Society of the Pacific (accepted, in press) (2015) 4 N. Jovanovic, F. Martinache, O. Guyon, C. Clergeon, G. Singh, T. Kudo, V. Garrel, K. Newman, D. Daughty, J. Lozi, J. Males, Y. Minowa, Y. Hayano, N. Takato, J. Morino, J. Kühn, E. Serabyn, B. Norris, P. Tuthill, G. Schworer, P. Stewart, L. Close, E. Huby, G. Perrin, S. Lacour, L. Gauchet, S. Vievard, N. Murakami, F. Oshiyama, N, Baba, T. Matsuo, J. Nishikawa, M. Tamura, O. Lai, F. Marchis, G. Duchene1, T. Kotani, and J. Woillez, “The Subaru Coronagraphic Extreme Adaptive Optics System: Enabling Highcontrast Imaging on Solar-system Scales”, Publications of the Astronomical Society of the Pacific (in revision) (2015). 5 J. K. Wallace, S. Rider, E. Serabyn1, J. Kühn, K. Liewer, J. Deming, G. Showalter, C. Lindensmith, and J. Nadeau, “A robust, compact implementation of an off-axis digital holographic microscope”, Optics Express (in revision) (2015) 6 J. Kühn, B. Mennesson, E. Serabyn, K. Liewer, S. Martin, F. Loya and R. Millan-Gabet, “Exploring the 5-40 AU scales around AB Aurigae with the Palomar Fiber Nuller”, The Astrophysical Journal 800(55) (2015). 7 J. Kühn, B. Niraula, K. Liewer, J. K. Wallace, E. Serabyn, E. Graff, C. Lindensmith, and J. L. Nadeau, “A Mach-Zehnder digital holographic microscope with sub-micrometer resolution for imaging and tracking of marine micro-organisms”, Review of Scientific Instruments 85(12) (2014). 8 J. Kühn, E. Shaffer, J. Mena, B. Breton, J. Parent, B. Rappaz, M. Chambon, Y. Emery, P. Magistretti, C. Depeursinge, P. Marquet, and G. Turcatti , “ Label-free cytotoxicity screening assay by digital holographic microscopy”, Assay Drug Dev Technol. 11(2): 101107 (2013). 9 D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, P.J. Magistretti, and P. Marquet, “Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy”, Journal of Biomedical Optics 18(3): 036007 (2013). 4 10 M. F. Toy, J. Kühn, S. Richard, J. Parent, M. Egli, and C. Depeursinge, “Accelerated autofocusing of off-axis holograms using critical sampling”, Optics Letters 37(24): 50945096 (2012). 11 M. F. Toy, S. Richard, J. Kühn, A. Franco-Obregòn, M. Egli, and C. Depeursinge, “Enhanced robustness digital holographic microscopy for demanding environment of space biology”, Biomedical Optics Express 3(2): 313-326 (2012) 12 N. Pavillon, J. Kühn, C. Moratal, P. Jourdain, C. Depeursinge C, P. J. Magistretti, and P. Marquet, “Early cell death detection with digital holographic microscopy”, PlosOne 7(1): e30912 (9 pages) (2012). 13 Y. Delacrétaz, E. Shaffer, N. Pavillon, J. Kühn, F. Lang, and C. Depeursinge, “Endoscopic low coherence topography measurement for upper airways and hollow samples”, Journal of Biomedical Optics 15(6): 066014 (6 pages) (2010). 14 C. Pache, J. Kühn, K. Westphal, M. F. Toy, J. Parent, O. Büchi, A. Franco-Obregón, C. Depeursinge and M. Egli, “Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity”, Journal of Biomedical Optics 15(2): 026021 (9 pages) (2010). 15 N. Pavillon, A. Benke, D. Boss, C. Moratal, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti and P. Marquet, “Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopy”, Journal of Biophotonics 3(7): 432-436 (2010). 16 T. Colomb, S. Krivec, H. Hutter, A. A. Akatay, N. Pavillon, F. Montfort, E. Cuche, J. Kühn, C. Depeursinge and Y. Emery, “Digital holographic reflectometry”, Optics Express 18 (4), 3719-3731 (2010). 17 T. Colomb, N. Pavillon, J. Kühn, E. Cuche, C. Depeursinge and Y. Emery, “Extended depth-of-focus by digital holographic microscopy”, Optics Letters 35(11): 1840-1842 (2010). 18 E. Shaffer, N. Pavillon, J. Kühn and C. Depeursinge, “Digital holographic microscopy investigation of second harmonic generated at a glass/air interface”, Optics Letters 34, 2450-2452 (2009). 19 B. Rappaz, E. Cano, T. Colomb, J. Kühn, C. Depeursinge, V. Simanis, P. J. Magistretti and P. Marquet, “Non-invasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy”, Journal of Biomedical Optics 14, 034049 (5 pages) (2009). 20 N. Pavillon, C.S. Seelamantula, J. Kühn, M. Unser and C. Depeursinge, “Suppression of the zero-order term in off-axis digital holography through nonlinear filtering”, Applied Optics 48, H186-H195 (2009). 21 J. Kühn, F. Montfort, T. Colomb, B. Rappaz, C. Moratal, N. Pavillon, P. Marquet, and C. Depeursinge, “Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection”, Optics Letters 34(5): 653-655 (2009). 22 J. Kühn, F. Charrière, T. Colomb, E. Cuche, F. Montfort, Y. Emery, P. Marquet and C. Depeursinge, “Axial sub-nanometer accuracy in digital holographic microscopy”, Measurement Science and Technology 19, 074007 (8 pages) (2008). 5 23 J. Kühn, T. Colomb, F. Montfort, F. Charrière, Y. Emery, E. Cuche, P. Marquet and C. Depeursinge, “Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition”, Optics Express 15, 7231-7242 (2007). 24 A. Marian, F. Charrière, T. Colomb, F. Montfort, J. Kühn, P. Marquet and C. Depeursinge, “On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography”, Journal of Microscopy 225, 156-169 (2007). 25 F. Charrière, B. Rappaz, J. Kühn, T. Colomb, P. Marquet and C. Depeursinge, “Influence of shot noise on phase measurement accuracy in digital holographic microscopy”, Optics Express 15, 8818-8831 (2007). 26 F. Montfort, T. Colomb, F. Charrière, J. Kühn, P. Marquet, E. Cuche, S. Herminjard and C. Depeursinge, “Submicrometer optical tomography by multiple-wavelength digital holographic microscopy”, Applied Optics 45, 8209-8217 (2006). 27 F. Charrière, J. Kühn, T. Colomb, F. Montfort, E. Cuche, Y. Emery, K. Weible, P. Marquet and C. Depeursinge, “Characterization of microlenses by digital holographic microscopy”, Applied Optics 45, 829-835 (2006). 28 F. Charrière, A. Marian, F. Montfort, J. Kühn, T. Colomb, E. Cuche, P. Marquet and C. Depeursinge, “Cell refractive index tomography by digital holographic microscopy”, Optics Letters 31, 178-180 (2006). 29 T. Colomb, E. Cuche, F. Charrière, J. Kühn, N. Aspert, F. Montfort, P. Marquet and C. Depeursinge, “Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation”, Applied Optics 45, 851863 (2006). 30 T. Colomb, J. Kühn, F. Charrière, C. Depeursinge, P. Marquet and N. Aspert, “Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram”, Optics Express 14, 4300-4306 (2006). 31 T. Colomb, F. Montfort, J. Kühn, N. Aspert, E. Cuche, A. Marian, F. Charrière, S. Bourquin, P. Marquet and C. Depeursinge, “Numerical parametric lens for shifting, magnification and complete aberration compensation in digital holographic microscopy”, Journal of the Optical Society of America A-Optics Image Science and Vision 23, 3177-3190 (2006). 6 Conference proceedings 1 M. Bottom, C. Shelton, J. Wallace, J. Kühn, B. Mennesson, R. Bartos, R. Burruss, M. Mawet, and E. Serabyn, “The SDC: high contrast imaging with a multistage vortex coronagraph”, 225th AAS Meeting, Kissimmee, USA (2015) 2 J. Kühn, B. Mennesson, K. Liewer, S. Martin, F. Loya, R. Millan-Gabet and E. Serabyn, “Additional insights on the solar system scale around AB Aurigae with the Palomar Fiber Nuller”, In Proceedings of SPIE 9146, Montreal, Canada (2014). 3 D. Mawet, C. Shelton, J. Wallace, M. Bottom, J. Kühn, B. Mennesson, R. Burruss, R. Bartos, L. Pueyo, A. Carlotti, and E. Serabyn, “Demonstration of vortex coronagraph concepts for on-axis telescopes on the Palomar Stellar Double Coronagraph”, Proceedings of SPIE 9143, Montreal, Canada (2014). 4 G. Singh, O. Guyon, P. Baudoz, N. Jovanovich, F. Martinache, T. Kudo, E. Serabyn, and J. G. Kühn, “Lyot-based low order wavefront sensor: implementation on the Subaru Coronagraphic Extreme Adaptive Optics System and its laboratory performance”, Proceedings of SPIE 9148, Montreal, Canada (2014). 5 N. Jovanovic, O. Guyon, F. Martinache, C. Clergeon, G. Singh, T. Kudo, K. E. Newman, J. Kühn, et al., “Development and recent results from the Subaru Coronagraphic Extreme Adaptive Optics system”, In Proceedings of SPIE 9147, Montreal, Canada (2014). 6 D. Defrère, O. Absil, P. Hinz, D. Mawet, J. Kühn, B. Mennesson, A. Skemer and K. Wallace, ”L’-band AGPM vector vortex coronagraph’s first light on LBTI/LMIRCAM”, In Proceedings of SPIE 9148, Montreal, Canada (2014). 7 D. Defrère, O. Absil, P. Hinz, D. Mawet, J. Kühn, B. Mennesson, A. Skemer and K. Wallace, ”L’-band AGPM vector vortex coronagraph’s first light on LBTI/LMIRCAM”, In Search for Life Beyond the Solar System. Exoplanets, Biosignatures & Instruments/International Journal of Astrobiology (special issue) P4.75, Tucson, USA (2014). 8 B. Niraula, J. L. Nadeau, E. Serabyn, J. K. Wallace, K. Liewer, J. Kühn, E. Graff, and C. Lindensmith, “A Mach-Zender Holographic Microscope for Quantifying Bacterial Motility”, American Geophysical Union, Fall Meeting P53A-4004, San Franciso, USA (2014). 9 J. L. Nadeau, C. Lindensmith, J. W. Deming, R. Stocker, E. Graff, E. Serabyn, J. K. Wallace, K. Liewer, and J. Kühn, “Bacterial Motility As a Biosignature: Tests at Icy Moon Analogue Sites”, American Geophysical Union, Fall Meeting P53A-4005, San Franciso, USA (2014). 10 R. Burruss, S. Hinkley, M. Wahl, J. Kühn, E. Cady, R. Dekany, B. Mennesson, S. Metchev, B. Oppenheimer, R. Patel, J. Roberts, E. Serabyn, J. C. Shelton, J. Tesch, G. Vasisht, “First exoplanet and disk results with the PALM-3000 adaptive optics system”, In Proceedings of AO4ELT III, Florence, Italy (10 pages) (2013). 11 M. F. Toy, S. Richard, J. Kühn, A. Franco-Obregon, M. Egli, and C. Depeursinge, “Digital holographic microscopy for the cytomorphological imaging of cells under zero gravity”, In Proceedings of SPIE 8227, 82270S (6 pages) (2012). 12 D. Boss, J. Kühn, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Quantitative measurement of absolute cell volume and intracellular integral refractive index (RI) with dual-wavelength digital holographic microscopy (DHM)”, In Proceedings of SPIE 8227, Brussels, Belgium (2012). 7 13 D. Boss, J. Kühn, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Dual-wavelength digital holography for quantification of cell volume and integral refractive index (RI)”, In Proceedings of SPIE 8086, 808608 (4 pages) (2011). 14 N. Pavillon, J. Kühn, P. Jourdain, C. Depeursinge, P. J. Magistretti, and P. Marquet, “Cell death detection and ionic homeostasis monitoring with digital holographic microscopy”, In Proceedings of SPIE 8090, 809004 (6 pages)(2011). 15 P. Marquet, J. Kühn, D. Boss, P. Jourdain, P. Magistretti, and C. Depeursinge, “Measuring biophysical properties of living cells with digital holographic microscopy”, In 2011 10th Workshop on Information Optics, WIO (2011). 16 P. Marquet, D. Boss, J. Kühn, P. Jourdain, C. Depeursinge, and P. Magistretti, “Exploring cell dynamics at nanoscale with digital holographic microscopy for diagnostic purposes”, Proceedings of SPIE 7690, Orlando, USA (20101). 17 P. Marquet, J. Kühn, D. Boss, P. Jourdain, P. Magistretti, N. Pavillon, and C. Depeursinge, “Progress and perspectives in digital holographic microscopy applied to life sciences”, In 9th Euro-American Workshop on Information Optics, WIO, Code 82123, Helsinki, Finland (2010). 18 M. F. Toy, C. Pache, J. Parent, J. Kühn, M. Egli and C. Depeursinge, “Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity”, In Proc. SPIE, 75700A. Vol. 7570, San Francisco, USA (2010). 19 J. Kühn, E. Solanas, S. Bourquin, J.-P. Blaser, L. Dorigatti, T. Keist, Y. Emery, and C. D. Depeursinge, ”Fast noncontact surface roughness measurements up to the micrometer range by dual-wavelength digital holographic microscopy”, In Proc. SPIE, 771805. Vol. 7718, Brussels, Belgium (2010). 20 N. Pavillon, J. Kühn, F. Charrière, and C. D. Depeursinge, “Optical tomography by digital holographic microscopy”, In Novel Optical Instrumentation for Biomedical Applications IV, Vol. 7371, SPIE, Munich, Germany (2009). 21 T. Colomb, J. Kühn, C. D. Depeursinge and Y. Emery, “Several micron-range measurements with sub-nanometric resolution by the use of dual-wavelength digital holography and vertical scanning”, In SPIE Proceedings 73891H, Vol. 7389, Munich, Germany (2009). 22 J. Kühn, F. Montfort, T. Colomb, B. Rappaz, C. Moratal, N. Pavillon, P. Marquet, and C. Depeursinge. “Tomography of Red Blood Cells by Multiple-Wavelength Digital Holographic Microscopy”, In Digital Holography and Three-Dimensional Imaging, DTuA3, Optical Society of America, Vancouver, Canada (2009). 23 E. Shaffer, N. Pavillon, J. Kühn and C. D. Depeursinge, “Second Harmonic and Fundamental Wavelength Digital Holographic Microscopy”, In Digital Holography and ThreeDimensional Imaging, JTuA3, Optical Society of America, Vancouver, Canada (2009). 24 C. Depeursinge, I. Bergöend, N. Pavillon, J. Kühn, T. Colomb, E. Cuche, and Y. Emery, “Measuring Shape and Surfaces down to the Nanometer and Nanosecond scales by Digital Holographic Microscopy”, Fringe 2009, 6th International Workshop on Advanced Optical Metrology, Nürtingen, Germany (2009). 8 25 J. Kühn, T. Colomb, C. Pache, F. Charriére and C. Depeursinge, “Sub-Nanometer Resolution over Several Microns Range with Real-Time Dual-Wavelength Digital Holographic Microscopy”, In Digital Holography and Three-Dimensional Imaging, DTuC2, Optical Society of America, St. Petersburg, USA (2008). 26 C. Depeursinge, N. Pavillon, J. Kühn, T. Colomb and P. Marquet, “Refractive Index Tomography by Digital Holographic Microscopy”, In Digital Holography and ThreeDimensional Imaging, DTuC3, Optical Society of America, St. Petersburg, USA (2008). 27 J. Kühn, F. Charrière, T. Colomb, F. Montfort, E. Cuche, Y. Emery, P. Marquet and C. Depeursinge, “Dual-wavelength digital holographic microscopy with sub-nanometer axial accuracy”, In Optical Micro- and Nanometrology in Microsystems Technology II, SPIE 6995, 99503, Strasbourg, France (2008). 28 B. Rappaz, E. Cano, J. Kühn, T. Colomb, C. Depeursinge, P. J. Magistretti and P. Marquet, “Non-invasive dry mass determination and monitoring at the single cell level with digital holographic microscopy”, In Progress in Biomedical Optics and Imaging, Proceedings of SPIE 699111, Vol. 6991, Strabourg, France (2008). 29 J. Kühn, T. Colomb, C. Pache, F. Charrière, F. Montfort, E. Cuche, Y. Emery, P. Marquet and C. Depeursinge,“Real-time dual-wavelength digital holographic microscopy for extended measurement range with enhanced axial resolution”, In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, SPIE 6861, 68610J8, San Jose, USA (2008). 30 T. Colomb, F. Charrière, J. Kühn, P. Marquet and C. Depeursinge, “Advantages of digital holographic microscopy for real-time full field absolute phase imaging”, In ThreeDimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, SPIE 6861, 686109-686110, San Jose, USA (2008). 31 F. Charrière, J. Kühn, T. Colomb, E. Cuche, P. Marquet, and C. Depeursinge, “Subcellular quantitative optical diffraction tomography with digital holographic microscopy”, In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 6441, San Jose, USA (2007). 32 J. Kühn, F. Charrière, T. Colomb, E. Cuche, Y. Emery and C. Depeursinge, “Digital holographic microscopy for nanometric quality control of micro-optical components”, In Proceedings of SPIE 6475, San Jose, USA (2007). 33 B. Rappaz, A. Barbul, F. Charrière, J. Kühn, P. Marquet, R. Korenstein, C. Depeursinge and P. Magistretti, “Erythrocytes volume and refractive index measurement with a digital holographic microscope”, In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 6445, San Jose, USA (2007). 34 F. Montfort, F. Charrière, J. Kühn, T. Colomb, E. Cuche, Y. Emery, P. Marquet and C. Depeursinge, “Multi-wavelength digital holographic microscopy for sub-micron topography of reflecting specimens”, In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 6443, San Jose, USA (2007). 35 T. Colomb, F. Charrière, J. Kühn, Y. Bellouard and C. Depeursinge, “Real-Time Birefringence Measurement with Digital Holographic Microscopy”, In Adaptive Optics: Analysis and Methods/Computational Optical Sensing and Imaging/Information Photonics/Signal Recovery and Synthesis Topical Meetings, paper-Dmb6, Optical Society of America, Vancouver, Canada (2007). 9 36 T. Colomb, F. Charrière, J. Kühn, F. Montfort and C. Depeursinge, “Numerical Optics in Digital Holography”, In Adaptive Optics: Analysis and Methods/Computational Optical Sensing and Imaging/Information Photonics/Signal Recovery and Synthesis Topical Meetings, DTuC4, Optical Society of America, Vancouver, Canada (2007). 37 J. Kühn, T. Colomb, F. Montfort, F. Charrière and C. Depeursinge, “Real-time dualwavelength digital holographic microscopy with a single hologram”, In Proceedings of SPIE, Vol. 6616, Munich, Germany (2007). 38 T. Colomb, F. Charrière, J. Kühn, F. Montfort and C. Depeursinge, “A complete digital optics applied to digital holographic microscopy: Application to chromatic aberration compensation”, In Proceedings of SPIE - The International Society for Optical Engineering, Vol. 6616, Munich, Germany (2007). 39 B. Rappaz, A. Barbul, F. Charrière, J. Kühn, P. Marquet, R. Korenstein, C. Depeursinge and P. Magistretti, “Erythrocytes analysis with a Digital Holographic Microscope”, In Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 6631, Munich, Germany (2007). 40 C. Depeursinge, T. Colomb, Y. Emery, J. Kühn, F. Charrière, B. Rappaz, and P. Marquet, “Digital holographic microscopy applied to life sciences”, 29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC’07, 62436246, Lyon, France (2007). 41 J. Kühn, T. Colomb, F. Montfort, F. Charrière, Y. Emery, E. Cuche, P. Marquet and C. Depeursinge, “Real-time dual-wavelength digital holographic microscopy for MEMS characterization”, In Conference on Optomechatronic Sensors and Instrumentation III, SPIE Proceedings 6716, 71608, Lausanne, Switzerland (2007). 42 F. Charrière, F. Montfort, J. Kühn, T. Colomb, A. Marian, E. Cuche, P. Marquet and C. Depeursinge, “Use of digital holographic microscopy in tomography - art. no. 61910O”, In Biophotonics and New Therapy Frontiers, O1910, Vol. 6191, SPIE-Int Society Optical Engineering, Strasbourg, France (2006). 43 P. Marquet, B. Rappaz, T. Colomb, F. Charrière, J. Kühn, Y. Emery, E. Cuche, C. Depeursinge and P. Magistretti, “Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics - art. no. 61910U”, In Biophotonics and New Therapy Frontiers, U1910, Vol. 6191, SPIE-Int Society Optical Engineering, Strasbourg, France (2006). 44 T. Colomb, J. Kühn, E. Cuche, F. Charrière, F. Montfort, A. Marian, N. Aspert, P. Marquet and C. Depeursinge, “Automatic procedure for aberrations compensation in digital holographic microscopy - art. no. 618805”, In Optical Micro- and Nanometrology in Microsystems Technology, 18805, Vol. 6188, SpieiInt Society Optical Engineering, Strasbourg, France (2006). 45 J. Kühn, E. Cuche, Y. Emery, T. Colomb, F. Charrière, F. Montfort, M. Botkine, N. Aspert and C. Depeursinge, “Measurements of corner cubes microstructures by highmagnification Digital Holographic Microscopy - art. no. 618804”, In Optical Micro- and Nanometrology in Microsystems Technology, SPIE Proceedings 6188, 18804-18804, Strasbourg, France (2006). 46 Y. Emery, E. Cuche, F. Marquet, N. Aspert, P. Marquet, J. Kühn, M. Botkine, T. Colomb, F. Montfort, F. Charrière, C. Depeursinge, P. Debergh and R. Conde, “Digital Holographic 10 Microscopy (DHM) for metrology and dynamic characterization of MEMS and MOEMS”, In Mems, Moems, and Micromachining II, N1860, Vol. 6186, SPIE-Int Society Optical Engineering, Strasbourg, France (2006). 47 F. Montfort, F. Charrière, T. Colomb, J. Kühn, E. Cuche and C. Depeursinge, “Tomography using multiple wavelengths in digital holography: Method, simulations and experiments - art. no. 618802”, In Optical Micro- and Nanometrology in Microsystems Technology, 18802, Vol. 6188, SPIE-Int Society Optical Engineering, Strasbourg, France (2006). 48 T. Colomb, J. Kühn, F. Charrière, P. Marquet, N. Aspert and C. Depeursinge, “Real-Time Phase Recovery of Biologicall Cell in Digital Holographic Microscopy by Use of a SelfCalibration Hologram”, In Biomedical Optics, paper-TuI47, Optical Society of America, Fort Lauderdale, USA (2006). 49 P. Marquet, P. Magistretti, B. Rappaz , T. Colomb, J. Kühn and C. D. Depeursinge, “Quantitative Measurements of Dynamic Cell Morphometry and Intracellular Integral Refractive Index with Digital Holographic Microscopy”, In Biomedical Optics, paper-Me24, Optical Society of America, Fort Lauderdale, USA (2006). 50 F. Montfort, Y. Emery, E. Solanas, E. Cuche, N. Aspert, P. Marquet, C. Joris, J. Kühn and C. Depeursinge, “Surface roughness parameters measurements by Digital Holographic Mcroscopy (DHM)”, In Proceedings of SPIE - The International Society for Optical Engineering, Vol. 6280 I, Urumqi, China (2006). 51 C. D. Depeursinge, A. M. Marian, F. Montfort, T. Colomb, F. Charrière and J. Kühn, “Digital Holographic Microscopy (DHM) applied to optical metrology: A resolution enhanced imaging technology applied to inspection of microscopic devices with subwavelength resolution”, In 5th International Workshop on Automatic Processing of Fringe Patterns (Fringe 2005), 308-314, Springer-Verlag Berlin, Stuttgart, Germany (2006). 52 Y. Emery, E. Cuche, F. Marquet, S. Bourquin, P. Marquet, J. Kühn, N. Aspert, M. Botkin and C. Depeursinge, “Digital Holographic Microscopy (DHM): Fast and robust 3D measurements with interferometric resolution for industrial inspection”, In 5th International Workshop on Automatic Processing of Fringe Patterns (Fringe 2005), 667-671, SpringerVerlag Berlin, Stuttgart, Germany (2006). 53 F. Charrière, J. Kühn, T. Colomb, F. Montfort, E. Cuche, Y. Emery, K. Weible and C. D. Depeursinge, “Microlenses metrology with digital holographic microscopy”, In Optical Measurement Systems for Industrial Inspection IV - SPIE, 447-453, Vol. 5856, Munich, Germany (2005). 54 Y. Emery, E. Cuche, F. Marquet, N. Aspert, P. Marquet, J. Kühn, M. Botkine, T. Colomb, F. Montfort, F. Charrière and C. Depeursinge, “Digital Holography Microscopy (DHM): Fast and robust systems for industrial inspection with interferometer resolution”, In Optical Measurement Systems for Industrial Inspection IV, 930-937, Vol. 5856, SPIE-Int Society Optical Engineering, Munich, Germany (2005). 55 C. Depeursinge, F. Charrière, A. M. Marian, T. Colomb, F. Montfort, J. Kühn, N. Aspert, M. Botkine, F. Marquet, S. Bourquin, E. Cuche, Y. Emery, P. Marquet and P. J. Magistretti, “Digital Holographic Microscopy, a new imaging technology applied to nanotechnology and life”, In Application of holographic and optical coherence methods, Proc. SPIE Holo 05, Varna, Bulgaria (2005). 11 56 C. D. Depeursinge, A. M. Marian, F. Montfort, T. Colomb, F. Charrière, J. Kühn, N. Aspert, M. Botkine, M. St-Ghislain, F. Marquet, E. Cuche, Y. Emery, P. Marquet, and P. J. Magistretti, “Digital Holographic Microscopy, a new imaging technology applied to biological cells and tissues”, In Conference on Lasers and Electro-Optics (CLEO) Europe, IEEE Technical Digest, 626, Munich, Germany (2005). 57 C. D. Depeursinge, F. Charrière, A. M. Marian, F. Montfort, T. B. Colomb, J. Kühn, E. Cuche, Y. Emery, P. Marquet and P. J. Magistretti, “Digital Holographic Microscopy applied to Metrology”, In Optical Metrology in Production Engineering, 504-512, Vol. 5457, SPIE-Int Society Optical Engineering, Strasbourg, France (2004). Patents 1 N. Pavillon, J. Kühn, P. Marquet, E. Cuche, P. Jourdain, C. Depeursinge and P. J. Magistretti, “Apparatus and method for early diagnosis of cell death”, PCT/IB2010/053768 (2010). 2 T. Colomb, E. Cuche, N. Aspert, J. Kühn, P. Marquet, C. Depeursinge, F. Montfort, F. Charrière, A. Marian, S. Bourquin, Y. Emery and S. Herminjard, “Wave Front Sensing Method And Apparatus”, US7649160, WO2006090320 (2006). Book chapters 1 T. Colomb and J. Kühn, “Digital Holographic Microscopy”, In Optical Measurement of Surface Topography, Chapter 10 (209-235), Springer, Berlin (2011). 12