Replace the 10-port rotor seal in the nanoLC Classic or TEMPO Required parts: For a 10-port valve with a titanium stator: 200-00159 Replacement rotor seal for Titanium 10-port valve A replacement stator only is not available for this configuration. Please replace the entire valve. See the document “Replace the 10-port valve”. For a 10-port valve with a PEEK stator: 200-00211 Replacement rotor seal for PEEK 10-port valve 200-00212 Replacement stator for PAEK 10-port valve Required tools: 9/16” hex wrench The stator is the front portion of the valve, containing the ports. The rotor seal is behind the stator and forms the liquid seal between the ports of the valve. If either of these parts is damaged, then sample or gradient delivery issues can occur. 1. Locate the two bolts that secure the stator of the valve to the valve body. See Figure 1. Figure 1 – valve with stator bolts indicated 2. Remove any fittings necessary that would prohibit the removal of the stator bolts. Mark these connections with the port number for easy replacement. 3. 4. 5. 6. Completely loosen the two bolts using a 9/16” hex wrench. Gently lift the stator from the valve body. The rotor seal in now exposed. Gently pry it out of the valve body. Replace the rotor seal with a new one. It will only fit in one orientation. See Figure 2. Figure 2 - rotor seal, showing sealing side 7. Press the new rotor seal firmly into place. 8. Inspect the sealing side of the stator for scratches or damage. Replace if necessary, as any scratches on the sealing surface may cause leaks. See Figure 3. If a titanium stator is damaged, replace the entire valve. See the Replace the 10-port valve document. Figure 3 – sealing side of stator 9. Fit the stator onto the stator pegs, with the bolt-holes aligned. 10. Fasten the stator to the valve body with the bolts and tighten securely. Do not over tighten, especially with a PEEK stator as it can crack! 11. Open the Eksigent software. 12. Click System / Direct Control. 13. Select the channel that controls the valve (usually channel 2). 14. Switch the valve between Load and Inject about 10 times to verify that the valve is functional and to set the valve stop positions.