INSTRUCTIONS
®
Tri-Sil TP
TS-49230 TS-49231
0909.1
Number
Description
TS-49230
Tri-Sil® TP, 10 x 1 ml ampules
TS-49231
Tri-Sil® TP, 25 ml Hypo-Vial⎢ Sample Storage Vial
Storage: Upon receipt store at 4°C. Product is shipped at ambient temperature.
This product is guaranteed for one year from the date of purchase when handled and stored properly.
Introduction
Tri-Sil TP is a mixture of trimethylsilylimidazole (TMSI) in dry pyridine (1.5 mEq/ml = 1 part TMSI: 4 parts pyridine) and is
used primarily for derivatizing hydroxyl compounds, particularly carbohydrates, and does not react with amines. Tri-Sil TP
can be used in the presence of water as long as there is enough reagent to react with both the water and the sample. The
reagent reacts with water in a 2:1 ratio. Tri-Sil TP has been used successfully for the silylation of the following compounds:
alcohols and phenols
amino acids
barbituarates
carbohydrates
indoles
vitamins
flavonoids
hydroxylamines
nucleotides
steroids
hydroxyl acids
narcotics
glycols and polyglycols
organic acids
Example Protocols
Protocol 1
1. Combine 10-15 mg sample and 1.0 ml Tri-Sil TP in a 3.0 ml Reacti-Vial Small Reaction Vial.
2. Cap vial and shake to dissolve. If necessary, heat at 60-70°C. Silylation is complete upon dissolution.
3.
Analyze by gas chromatography.
Protocol 2
For solutions containing ~1% or less total sugars, use a 50:50 v/v TMSI/pyridine solution.
1. Evaporate ~50 ∝l sugar solution to a glassy syrup in a 0.3 ml Reacti-Vial Small Reaction Vial.
2. Add 50 μl TMSI/pyridine solution.
3. Cap vial and heat at 60°C to dissolve and derivatize the sugars.
4. Analyze directly by GC (% OV-1 or OV-17) for full-scale peaks at mid-range sensitivity settings on FIDs.
References
Hymovwitz, T., et al. (1972). Relationship between the content of oil, protein and sugar in soybean seed. Agronomy J. 64:613.
Low, N., et al. (1994). Normative data for commercial pineapple juice from concentrate. J. of AOAC Intl. 77:965.
Quilliam, M.A., et al. (1980). Study of rearrangement reactions occurring during gas chromatography of tert-butyldimethylsilyl ether derivatives of uridine.
J. Chrom. 194:379.
Current versions of product instructions are available at www.thermo.com/columns.
© 2008 Thermo Fisher Scientific Inc. All rights reserved. Unless otherwise indicated, all trademarks are property of Thermo Fisher Scientific Inc. and its
subsidiaries. Printed in the USA.
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