Proceedings of DEPARTMENT OF BOTANY, Telangana University (A State University Established under the Act No.28 of 2006, A.P. Recognized by UGC under 2(f) and 12 (B) of UGC Act 1956) Accredited with B Grade by NAAC Dichpally, Nizamabad, T.S. International E – Publication www.isca.me , www.isca.co.in Proceedingsof National Seminar on MEDICINAL PLANTS FOR BETTER HUMAN HEALTH January 28 -29, 2016 SPONSORED BY Indian Council of Medical Research, New Delhi, Science and Engineering Research Board, New Delhi Telangana State Council of Higher Education, Hyderabad Organized By Department of Botany, Telangana University (A State University Established under the Act No.28 of 2006, A.P. Recognized by UGC under 2(f) and 12 (B) of UGC Act 1956) Accredited with B Grade by NAAC Dichpally, Nizamabad, T.S. Editors Prof. B. Vidya Vardhini Professor and Head, Dept. of Botany, Telangana University Dr. Ahmed Abdul Haleem Khan Assistant Professor, Dept. of Botany, Telangana University 2016 International E - Publication www.isca.me , www.isca.co.in International E - Publication 427, Palhar Nagar, RAPTC, VIP-Road, Indore-452005 (MP) INDIA Phone: +91-731-2616100, Mobile: +91-80570-83382 E-mail: contact@isca.co.in , Website: www.isca.me , www.isca.co.in © Copyright Reserved 2015 All rights reserved. No part of this publication may be reproduced, stored, in a retrieval system or transmitted, in any form or by any means, electronic, mechanical, photocopying, reordering or otherwise, without the prior permission of the publisher. ISBN:978-93-84659-27-1 Proceedings of National Seminar on Medicinal Plants For Better Human Health iii PREFACE The department of Botany started when the University was established in the year 2006. The Knowledge of the plants has always played a key role for mankind and their development since ages. Botany deals with classification, anatomy, physiology and biochemistry and even the molecular biology of the plants and their important role in human life. Botany is course that gives insight about the importance of plants; it has several branches, which deal with basics as well as applied aspects to meet the demand of scientific necessities of contemporary world. We are organizing a 2-Day National Seminar entitled “Medicinal Plants for Better Human Health” sponsored byTelangana State Council of Higher Education (TSCHE), Indian Council of Medical Research (ICMR), New Delhi and SERB (Science and Engineering Research Board), Department of Science and Technology, Government of India on 28-29 Jan 2016. The two day event includes an inaugural session, 4 plenary sessions and a valedictory session. Each plenary session includes two lectures and will be chaired by the faculty of the university. The two day National Conference will commence on 28 th January around 10:00 am, the Hon’ble Vice-Chancellor, Shri C. Parthasarathi (IAS) will be the Chief Guest and the Dynamic Registrar Prof. R. Limbadri will be the Guest of Honour. Plenary Session–I: Dr. B. Dinesh Kumar, Dr. Sudhakar Reddy Plenary session-II: Prof. K. Satya Prasad, Prof. S. Seeta Ram Rao,Prof. S. Jeelani Plenary Session –III:Prof. Sahera Nasreen, Prof. K. Seshagirirao Plenary Session –IV: Prof. Mohan Karupayil In this National Event around 80 abstracts were received on the various aspects of medicinal plants that are very helpful in human health viz., taxonomy of medicinal plants, ethnomedicinal plants traditional knowledge on herbal medicines, extraction techniques for medicinal and aromatic plants , conservation of medicinal plants , biotechnology of medicinal plants, bioprospecting of medicinal plants for herbal drugs, bioactive lead molecules from medicinal plants, phytotherapy, medicinal plants & nanomedicine etc. will be presented by delegates coming from various universities. We are grateful to the Hon’ble Vice-Chancellor, Shri C. Parthasarathi (IAS), for his moral support in organizing this National Seminar. We are grateful to the Dynamic Registrar, Prof. R. Limbadri, for his support. We are also grateful to Prof. Naseem, Dean, CDC, Prof. Y. Jaya Prakash Rao, Dean Faculty of Sciences and Prof. P. Kanakaiah, Principal, University College for their consistent encouragement to the organizers. We are also grateful to the Advisors, Members of the Executive Committee and Members of the organizing committee for their constant help and support. Prof. B. Vidya Vardhini Convener Dr. A. A. Haleem Khan Organizing Secretary International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health iv ABOUT THE SEMINAR Our country has long history of traditional medicine used to treat several acute and chronic diseases by herb practitioners in rural hamlets. These medicines are propagated through cultural patrimony. Around the globe these medicines are common to treat ailments and maintain human health. Medicinal plants are well known to be part of alternative medicines popular among community based and self-supported health care. These plants offer research areas of phytochemistry (characterized bioactive compounds), pharmacognosy (assays of bioactivity, identification of mode of action and target sites for compounds), and horticulture (develop the optimum growth in cultivars). The commercial drug manufacturers investigate the bioactive compounds and pharmacological activities in the traditional medicine. The low cost medicine used as folklore turns very expensive with brand name. The drugs available in market today are prepared by gaining knowledge from the traditional healing system. The nutraceuticals with improved medicinal qualities are primary focus of research that could benefit the health. The plants with nutritional value and without nutritional value are studied to overcome acute or chronic health problems. The phytochemical constituents incorporated in the nutraceuticals for health aid developed functional foods. Hence this seminar will open avenues for deliberations, discussions and finally to formulate idea among the young researchers who take medicinal plants as their research area. This platform helps to gain access with eminent researchers from esteem institutes. The understanding of igniting minds activates the community to realize and shoulder the responsibilities for preservation of plant biodiversity. The students are expected to disseminate the conservation message amongst the rural masses, countryside where there is its dire need. FOCAL THEMES OF THE SEMINAR Taxonomy of medicinal plants, Ethnomedicinal plants Traditional knowledge on herbal medicines Extraction Techniques for Medicinal and Aromatic Plants Conservation of medicinal plants Biotechnology of medicinal plants Bioprospecting of medicinal plants for herbal drugs Bioactive lead molecules from medicinal plants Phytotherapy, Medicinal plants & Nanomedicine International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health v Contents S. No. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Title, Authors & Affiliations Safety Evaluation of Traditional Preparations Dr. B. Dinesh Kumar, Centre for Advanced Research for Pre-clinical toxicology (PCT) & Drug Toxicology Division Food and Drug Toxicology Research Centre National Institute of Nutrition, Hyderabad Conspectus on Modern Medicinal Botany Kottapalli Seshagirirao Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Enhancement of quantitative and qualitative yield of medicinal and aromatic plants by brassinosteroids S.Seeta Ram Rao* Department of Botany, Osmania University, Hyderabad Ayurveda Inspired Drug Discovery: Promises and Challenges S. Mohan Karuppayil, Professor, School of Life Sciences (UGC SAP and DST FIST Sponsored), SRTM University, Nanded Medicinal Plants-A Boon for Human Health Sahera Nasreen Department of Botany, P.G. and Research center, Government Institute of Science, Nipat Niranjan nagar, Caves Road, Aurangabad-431004 Pharmacognostic Study of Herbal Medicine: Challenges and Opportunities Prof. S. Jeelani Director, Centre for Virtual Learning (CDVL), University of Hyderabad, Remote sensing applications in Biodiversity and conservation C. Sudhakar Reddy* Forestry and Ecology Group, National Remote Sensing Centre, Balanagar, Hyderabad Exploration of ‘Mode of action’ of Phytochemicals as Antioxidants and Chelating agents Dr. Y. Srinivasa Reddy, Dr. T. Manjula and Dr. B. Dinesh Kumar Food and Drug Toxicology Research Centre National Institute of Nutrition Pivotal Role of Medicinal Plants in Primary Healthcare of Human M. Aruna Dept. of Botany, Telangana University, Dichpally, Nizamabad Next-Generation RNA Sequencing for Gene Discovery in Medicinally Important Plants Praveen Mamidala Department of Biotechnology, Telangana University, Nizamabad Medicinal Plants for Better Human Health: Social Work Interventions K. Rajeshwari Department of Social Work, Telangana University, South Campus, Bhiknur STEVIA: The alternative for sugar A. Ravi Charan*, V. Prathap Reddy and Ranjit Nagireddy PRR Biotech Innovations Pvt. Ltd. Studies on Grass diversity of Karaikal District, U.T of Puducherry K. Sambandan, N. Dhatchanamoorthy and S. Kalaivani Department of Plant Science, AvvaiyarGovt College for Women, Karaikal, U.T of Puducherry Role of Endophyitic actinomycetes from Medicinal Plants in Dharwad region WaheedaKhanam and VootlaShyam Kumar Post Graduate Department of Biotechnology and Microbiology, Karnataka University, Dharwad580003, Karnataka Bio-Plastics a Long Way to Solve Environment Pollution A. Dharani Dept. of Library Science, GDC (W), Srikalahasthi International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Page No. 01 01 02 02 03 03 04 04 05 06 06 07 08 08 09 Proceedings of National Seminar on Medicinal Plants For Better Human Health 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. Efficiency of AgNPs in combined mode with drugs against pathogenic bacterial strains Anima Nanda FNRS Faculty of Bio & Chemical Engineering, Sathyabama University Rajiv Gandhi Salai, Chennai-600119, India Traditional knowledge for Modern Medicine K. Prasanna Rani* Dept. of Law, Telangana University, Dichpally, Nizamabad Biosynthesis Silver Nanoparticles from Syzygiumaramaticum and Their Antimicrobial Activity Siddiqui A G, Awasthi R S Dept. of Microbiology and Biotechnology, Dayanand Science College, Latur, Principal, Shivaji College, Renapur, Dist: Latur Standardization of Avarttani (HelicteresisoraLinn. Stem bark)-An Ayurvedic drug J. Shashikanth, P. Ramachandra Reddy* Plant Anatomy and Taxonomy Laboratory, Department of Botany, University College for Women, Koti, Osmania University, Hyderabad Evaluation of Regeneration Potential of Mature Seed Culture in an Anti-Diabetic Herbal Legume Crop- Trigonellafoenum-graecumL. Vikrant* and Karthiga, K. Post-Graduate Department of Plant Science, Avvaiyar Government College for Women, Karaikal609 602, U.T. of Puducherry, India. *Post-Graduate Department of Plant Science, Mahatma Gandhi Government Arts College, New Mahe In vivo anti-inflammatory effect of various extracts of Canthiumcoromandelicum (Burm. F.) Alston Priyadarshini S. Ekambe* and B. S. Surwase School of Life Sciences, S. R. T. M. University, Nanded- 431606, Maharashtra, India. Evaluation of Antioxidant Activity in different parts of Costusigneus(L.,) Elizabeth Margaret*, Dr (Mrs.) Kalpana& Ms. Roja Department of Botany, St. Ann’s College for Women, Mehdipatnam, Hyderabad. Isolation and Characterization of Anti-Alzheimer’s Compounds from Celastruspaniculatus B. Nirmala Kumari and N.Sudhakar 1. Department of Zoology, GDC (W), Srikalahasthi, Andhra Pradesh, India 2. Department of Microbiology, GDC (W), Srikalahasthi, Andhra Pradesh, India Detection and Identification of Pesticides in Fruits and Vegetables SyedaAzeemUnnisa Department of Environmental Science, University College of Science, Osmania University, Hyderabad Telangana State, India Herbal medicine: Parkinson's disease G. Swathi, Dept. of Zoology, Government Degree & PG College, Srikalahasthi Estimation of E&Z-guggulsterones and In vitro Propagation studies inCommiphorawightii (Arn.) Bhandari Ch. Mohan, D. Sreekanth, P. Manjula, B. Keerthi, B. Kiran Kumar and Prathibha Devi* Plant Biotechnology and Molecular Genetics Laboratory, Center for Advance Studies in Botany, Department of Botany, Osmania University, Hyderabad-500007, India. Traditional knowledge on Indian herbal Medicine S. Padmavathi, Department of Botany, GDC, Nagari. Airborne microfungi in rural environments of Puducherry region Bijaya Kumar Nayak Department of Botany, K. M. Centre for Post Graduate Studies (Autonomous), Lawspet, Airport Road, Pondicherry-605008, India Evaluation of Free Radical Scavenging Potential and Anti-cataract Activities of Selected Medicinal Plants 1 Dhole N. A.*, 2 Gacche R. N. 1 Digambarrao Bindu Arts, Commerce & Science College, Bhokar, Nanded-431801 2 School of Life Sciences, Swami RamanandTeerthMarathwada University, Nanded International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in vi 09 10 10 11 11 12 12 13 13 14 14 15 15 16 Proceedings of National Seminar on Medicinal Plants For Better Human Health 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. DNA – Welfare of Living Beings Madhamanchi Pradeep, Department of Biotechnology, Govt. Degree College (Men), Srikakulam KatiraGond- Novel Natural Product Srinivasa Reddy CH1, N V S SSatyadev T2, Manoranjani M2 1.Department of Botany, PB.Siddhartha College of A & S, Vijayawada 2.Department of Chemistry, P G Centre, PB.Siddhartha College of A & S, Vijayawada Management of Fusarium Wilt by Botanical pesticides in Chilies- an important commercial cash crop E. Shravyapuri*, A. Sabitha Rani Department of Botany, Osmania University College for Women, Koti, Hyderabad. Micropropagation Studies on Stem Node Explants of CucumissativusL. A Medicinal Important Plant M. Venkateshwarlu Department of Botany, Kakatiya University, Warangal Free radicals-Phytomedicine K. ChittiKalavathi, Dept. of History, GDC, Nagari. Preliminary screening of Endophytic fungi from Enicostemmaaxillare (Lam.) Raynal. for antimicrobial activity Yamilee Das*, Ujwala S. Deepake, G. Gyananath School of Life Sciences, Swami RamanandTeerthMarathwada University, Nanded-431606, Maharashtra, India. Science Teacher Education: Nurturing Young Minds for Better Health P. Samata&Pavan MNV University College of Education, Telangana University Observations on the Vegetation and Flora of Satkosia Tiger Reserve, Odisha K. Chandramohan1*, P.V. Prasanna1& P. Ramachandra Reddy2 1 Botanical Survey of India, Deccan Regional Centre, Hyderabad, Telangana-500048 2 Department of Botany, Osmania University, Hyderabad Effects of Biofertilizers on Yield and Biochemical Parameters of Ashwagandha (Withaniasomnifera) D. Vijaya Lakshmi, T. Prabhakar Reddy, J. Kamalakar, and C. Padma Veni KrishiVigyan Kendra, Rudrur, Nizamabad Professor Jayashankar Telangana State Agricultural University, Telangana. Antifungal Activity of Some Important Medicinal Plants against Two Plant Pathogenic Fungi Jalander, V., S. Prashanthi and M. Mamatha Department of Botany, Telangana University, Dichpally, Nizamabad The Promise of Insulin Plant in Treatment of Diabetes Syed AfzaluddinBiyabani*, Syed Raziuddin Faisal, Javed Akhtar Ansari Department of PharmD, MESCO College of Pharmacy, Hyderabad, TS. Heavy metals in Herbal preparations P. Manohar Dept. of Zoology, SVA Govt. Degree College, Srikalahasthi. Pollen analysis of honeys from Hukumpetamandal of Paderu forest division in Visakhapatnam district R. Devender and H. Ramakrishna* Department of Botany, University College of Science, Osmania University, Hyderabad Role of Coarse grains & Millets in Human Health– A Review A.Poshadri, G. Praveen Kumar* and J.Sai Prasad College of Food Science & Technology *Regional Sugarcane and Rice Research Station, Rudrur, Nizamabad. Professor Jayashankar Telangana State Agricultural University, Hyderabad, Telangana. Finance Commitees for AYUSH G. Padmavathy Customer Support Officer, State Bank of India, Miryalaguda, Telangana International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in vii 16 16 17 17 18 18 18 19 19 20 20 21 21 21 22 Proceedings of National Seminar on Medicinal Plants For Better Human Health 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 58. 59. Antifungal Effect of Some Weed Plant Leaf Extracts on Seed-borne Fungi of Pigeonpea [Cajanus cajan (L.) Millsp.]. Seeds Jalander, V. and B.D. Gachande* Department of Botany, Telangana University, Dichpally, Nizamabad (T.S.) *Botany Research Laboratory and Plant Disease Clinic, N.E.S. Science College, Nanded. Organic Farming of Medicinal and Aromatic Plants: A Hope for Quality Enhancement T. Prabhakar Reddy, D. Vijaya Lakshmi, J. Kamalakar and Dr. B. Joseph Regional Sugarcane and Rice Research Station, Rudrur, Nizamabad Professor Jayashankar Telangana State Agricultural University, Hyderabad, Telangana. Pharmacological Profile of Indian Solid Gold (Curcumin) Syed Raziuddin Faisal*, Syed AfzaluddinBiyabani, Javed Akhtar Ansari Department of PharmD, MESCO College of Pharmacy, Hyderabad, TS, India. Remediation of heavy metals effect on stomata cells in Curcuma longa L. Damera Vineeth1*, Chinna. Venkateshwar2 and SyedaAzeem Unnisa3* Department of Botany, University College of Science, Osmania University, Hyderabad Origin of Herbal medicine: A critical review G. Tirumala Vasu Deva Rao, Dept. of History, Government Degree College, Nagari Antimicrobial Activity of Some Important Medicinal Plants Jalander, V., A.A. Haleem Khan and B. VidyaVardhini Department of Botany, Telangana University, Dichpally, Nizamabad (T.S.) Phytonutrient and Antioxidant Enzyme Studies in Phoenix Fresh Ripened Fruit B.Thirupathi* and S. Gangadhar Rao. Department of Botany, Osmania University, Hyderabad Health Benefits of Green Tea A. Srilakshmi, Department of Computer Application, GDC (W), Srikalahasthi. In vitro production of Spilanthol – A medicinally important secondary metabolite from hairy root cultures of Spilanthesacmella Hajera Sana* and A.Sabitha Rani Dept. of Botany, Osmania University College for Women, Koti, Hyderabad Phytochemical Investigation of Curcuma longa M. Thejomoorthy Dept. of Zoology, GDC, Rajahmundry Medicinal Properties of Sapindaceae Family Devaraju Srinivas, K. Jaganmohan Reddy* Department of Botany, Telangana University, Dichpally, Nizamabad, (T. S.) *Department of Botany, Kakatiya University, Warangal, (T. S.) India In vitro antagonistic activity of Trichoderma harzianum and Trichoderma virideagainst Alternariaalternata, the leaf spot pathogen of Senna plant NagamaniTagaram and Bhumi Narasimha Reddy Department of Botany, Osmania University College for Women, Koti, Hyderabad. Medicinal Value of Rice Land Races in India N.Sandhyakishore*, G.Praveenkumar, M.Pallavi, T.Prabhakar Reddy, J.Kamalakar, FirdozShahana, Y.Bharathi and B.Joseph *Corresponding Author: N.Sandhyakishore, Scientist (Plant Breeding), Regional sugarcane and Rice Research Station, Rudrur, Nizamabad-503188. Effect of Aqueous Extracts of Datura sp. Against Fusarium oxysporumf.sp. udum, Wilt Pathogen of Pigeonpea [Cajanuscajan (L.) Millsp.]. Jalander, V. and B.D. Gachande* Department of Botany, Telangana University, Dichpally, Nizamabad (T.S.) *Botany Research Laboratory and Plant Disease Clinic, N.E.S. Science College, Nanded. Azadirachtaindica The Medicinal Plant in Poultry Birds Syeda Zainab, Maulana Azaad College, Dr BAMU, Aurangabad. M.S. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in viii 23 23 24 24 25 25 26 26 26 27 27 28 28 29 29 Proceedings of National Seminar on Medicinal Plants For Better Human Health 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. Phytochemical screening and in vitro anti-oxidant activity of Bacopamonnieri M. Sharmila Department of Zoology, GDC (W), Srikalahasthi. Phytochemical screening, antibacterial and antioxidant properties of Thevetiaperuviana(Pers.) K. Schum S. Thejaswi1* andM. Uma Devi2. 1 Department of Biochemistry, St Mary’s College, Hyderabad, Telangana. 2 Department of Biotechnology, Aurora College, Hyderabad, Telangana. Medicinal flora described in Lalitāsahasranāmastothramand their significance Devarinti Srihari Reddy, Govt. Degree College, Ramannapet, Nalgonda In vitro Propagation of Spine Gourd (Momordica dioica. Roxb.) V. Prathap Reddy, A. Ravi Charan, Ranjit Kumar Nagireddy PRR Biotech Innovations Pvt. Ltd. In vitro regeneration from different explants of Costuspictus - an insulin plant G.Sulakshana* and A.Sabitha Rani Department of Botany, Osmania University College for Women, Koti, Hyd. Study of Ethnomedicinal Plants of Bheemgal region of Nizamabad District T.Umakiran1 and M.Chandrakumar2 1. Department of Botany, Girraj Government College, Nizamabad. 2. Department of Zoology, Government Degree College, Nirmal. Medicinal Value of Rice Types for Human Health in India – A Review G. Praveen Kumar, A. Poshadri*, N. Sandhyakishore and B. Joseph. Regional Sugarcane and Rice Research Station, Rudrur, Nizamabad. *College of Food Science & Technology Professor Jayashankar Telangana State Agricultural University, Hyderabad, Telangana. Plant Tissue Culture Studies on Cocciniaindica-A Vegetable Crop Plant M. Venkateshwarlu(1) T.Ugendher(3) D.Srinivas(4)M.Nagaraju(1) P.Sreevani (2)and B.Mallaiah(1) (1)Department of Botany, Kakatiya University, Warangal – 506009 T.S. (2)Department of Physics, Kakatiya University, Warangal – 506009 T.S. (3)Department of Botany, S.R.R. PG College, Karimnagar – T.S. India (4)Department of Botany, Telangana University, Nizamabad–T.S. India Study of Medicinal Values and Health benefits of Mustard Plant P. Latha1 and B. VidyaVardhini*2 1 Lecturer, Govt. Degree College, Armoor, Nizamabad, Telangana State *2Dept. of Botany, Telangana University, Dichpally-503322, Nizamabad Plants of Adilabad Forest used by Hakim for the Treatment of Certain Diseases Shaik Hafeez and J.Chapla Department of Botany, University College of Science, Osmania University, Hyderabad. Antibacterial Activity in Methanolic Leaves Extract of Madhucaindica L. Yahya Khan and SaheraNasreen Department of Botany, P.G. and Research Center, Government Institute of Science, NipatNiranjan Nagar, Caves Road, Aurangabad -431004 (M.S.) Impact of Sulphur dioxide on medicinal value of Cassiatora T. Prasannarani1, D. U Gawai2 1 Dept. of Botany, Lal bahadur shastriMahavidyalaya, Dharmabad (M.S) 2 Dept. of Botany, Science College Nanded (M.S) Medicinal plants used to cure amoebiasis in Khammam District of Telangana, India Dr. RatnaManjula, R. Dept. of Botany, Government Degree College, Rammannapet, Nalgonda Medicinal plants used in the treatment of Diabetes by ethnic people of Nizamabad district, Telangana, India J. Vasudev and B. VidyaVardhini* Department of Botany, Telangana University, Dichpally, Nizamabad International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in ix 30 30 30 31 32 32 32 33 33 34 34 37 39 42 Proceedings of National Seminar on Medicinal Plants For Better Human Health 74. 75. 76. 77. 78. 79. 80. 81. 82. 83. 84. 85. 86. 87. 88. A study on Ethno medicinal Values of Plants used by Koyas in Tadvai Mandal, Warangal District, Telangana, India K. Anuradha Department of Botany, Government Degree College, Siddipet, Medak Dist. Effect of Salicylic acid on the growth of Cotton grown in Nizamabad District Y. Venkateshwarlu, B. VidyaVardhini Department of Botany, Telangana University, Dichpally.Nizamabad. (TS) Medicinal Plants of Nizamabad District with anti-diabetic potential Gaddam Srinivas Reddy, T.VenugopalaSwamy*, S.Prashanthi** Department of Zoology, Govt. Junior College for Boys, Nizamabad *Department of Zoology, Girraj Govt College, Nizamabad **Department of Botany, Telangana University Medicinal values of Euphorbiaceae Family in Nizamabad District, Telangana State S. Shireesha, Dr. A.A. Haleem Khan Department of Botany, Telangana University,Dichpally, .Nizamabad. (TS) Cultivation and Conservation of Medicinal Plants and Women Empowerment M.Saraswathi, Asst. Professor, Dept. of Economics, G.D.C.(M), Nirmal, Adilabad. Health Benefits of Withaniasomnifera L. R. Rajakala, Dr. A.A. Haleem Khan Department of Botany, Telangana University, Dichpally.Nizamabad. (TS) Role of Traditional Medicine in Primary Health Care and TMK in Developing Countries Sunil Kumar A Department of Zoology, Girraj Government College (A), Nizamabad Traditional uses and conservation of medicinal plants in Nizamabad district E. Kiran, B. VidyaVardhini* Department of Botany, Telangana University, Dichpally, Nizamabad Exotic Floristic Diversity of the Nizamabad District, Telangana State, India Vijigiri Dinesh Department of Botany, Shri Renukadevi Arts, Commerce and Science College, Mahur, Nanded, Maharashtra, India. Study of Various Medicinal Benefits of Spirulina on Human beings M. Srinivasa Rao1 and P. Latha2 1 Dept. of Microbiology Govt. Degree College, Armoor, Nizamabad, Telangana State 2 Dept. of Botany, Govt. Degree College, Armoor, Nizamabad, Telangana State A study of locally available medicinal plants of Ananthagiri hills forest area and their role in folklore medicine. Dr.N.C. Sowjanya, Dept. of Botany, Government City College (A), Hyderabad Micropropagation, molecular profiling and RP-HPLC determination of Woodfordin C &Woodfordin I across various regeneration stages of Woodfordiafruticosa (L.)Kurz MahenderAileni Dept. of Biotechnology, Telangana University, Dichpally, Nizamabad Isolation and identification of some pathogenic fungi from different infected vegetables DigambarS.Pawar and SaheraNasreen Dept.of Botany, Government Institute of Science and Research Center, NipatNiranjannagar, Caves road, Aurangabad-431004 Traditional knowledge on medicinal plants used in the treatment of mouth ulcers in rural villages of Mahabubnagar District, Telangana, India. P. Shivakumar Singh1, D.S.R. Rajender Singh2, G.M. Vidyasagar3 1 Department of Botany, Palamuru University, Mahabubnagar-509001, Telangana, India. 2 Principal, Department of Botany, SV Govt. UG & PG College, Palem, Mahabubnagar, 3 Medicinal Plants and Microbiology Research Laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, Gulbarga –585 106, Karnataka ABOUT EDITORS International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in x 45 48 49 51 51 55 55 57 58 59 59 59 60 63 68 Proceedings of National Seminar on Medicinal Plants For Better Human Health 1 Safety Evaluation of Traditional Preparations Dr. B. Dinesh Kumar, Deputy Director Sr. Gr. (Scientist ’F’) Coordinator –Centre for Advanced Research for Pre-clinical toxicology (PCT) & HOD – Drug Toxicology Division Food and Drug Toxicology Research Centre National Institute of Nutrition, Indian Council of Medical Research Jamai-Osmania, Tarnaka, Hyderabad-500007 Email:nindinesh@rediffmail.com, nindineshpct@gmail.com Phone No (Office): +91 (40) 2719 7322 The Ancient wisdom has been the basis of Modern medicine and will remain as one important source of medicine and therapeutics. It is well known that traditional medicine differ from country to country and region to region and is dependent on indigenous cultural practices. Since one decade there is a growing demand of natural products as potential preventive and therapeutic agents all over the world. Many international agencies especially WHO has now recognized integrated system of medicine which includes Ayurveda, Unani and Homeopathy to achieve health for all. It is therefore, necessary to generate the safety, efficacy quality profile of drugs as per the regulatory guidelines. The history of development of regulatory guidelines is a century old process and now become mandatory after the alarming reports viz. diphtheria toxin epidemic in 1901, toxic effects due to exposure of the addictive Drugs/Alcohol, Thalidomide catastrophe story in 1962 etc on the safety of drugs. The development of guidelines for safety evaluation of any preparations is a continuous process which is being developed from time to time by both National Agency (Drug Controller General of India) and International Agencies (World Health Organisation (WHO), Organization of Economic Cooperation and Development (OECD), International Conference of Harmonization (ICH), Food & Drug Administration U.S.A. (FDA)s etc.). The pharmaceutical industries are now realizing the changing attitude of masses towards the traditional/ indigenous/ natural preparations and are keen to promote such products having beneficial health effects. In addition as per WTO agreements it is not enough to promote, patent indigenous / traditional preparations, but get them tested for safety, efficacy etc. as per the prescribed international guidelines for wider global acceptance. The safety evaluation procedure especially for indigenous products is one of the prime important areas for academia and industry. Conspectus on Modern Medicinal Botany Kottapalli Seshagirirao Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad – 500046, Telangana State Medicinal Botany is (a neologism by Dr. K. Seshagirirao, University of Hyderabad, India in 2005: Internet search with keywords: Medicinal Botany & Dr. K. Seshagirirao) the subject deals with traditional and folk medicine practice with extended modern techniques including pharmaceutical science based on the use of plants and plant extracts. The bioinformatics related to this subject could be referred to as MedBotanics (Seshagirirao, 2005). The subject matters such as collection & storage of the plants (according to CITES, IUCN, CBD & FAO), analysis both traditional & modern aspects, biochemical & pharmaceutical analysis and the modern approaches would be discussed in this seminar. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 2 Enhancement of quantitative and qualitative yield of medicinal and aromatic plants by brassinosteroids S.Seeta Ram Rao* Department of Botany, Osmania University, Hyderabad-500 007. *E-mail: ssrrao2002@rediffmail.com Brassinosteroids are new generation plant hormones with growth promoting nature. Brassinosteroids are known to influence varied growth and developmental process in plants. The improvement of economic yield of cereals, vegetables, oil seed crops and fruit crops is well established. The papers discuss the importance of the brassinosteroids on the performance of certain medicinal and aromatic plants with special emphasis on pharmaceutically active substances. Exogenous application of brassinosteroids substantially improved the growth of rose-scented geranium (Pelargonium graveolens). Further there was significant rise in geroniol content and decrease in citranellol content reflecting the improvement of economic value of the aromatic acid. Similarly, brassinosteroid-feeding resulted is increase in tuber yield of Coleus (Coleus forskohlii). Further there was steep increase in forskolin, the pharmaceutically important ingredient in Coleus. Brassinosteroid application also resulted in improvement of root yield in ashwagandha (Withania somnifera). The growth promotion further translated in enhancement in the content of withaferin-A, the praised ingrediant of the plant. Brassinosteroid treatment as foliar spray caused impressive growth of Tinospora cordifolia as well as increase in total alkaloid content. Moreover aqueous extracts of brassinosteroid treated Tinospora plants exhibited higher antioxidant activity as compared to untreated control plants. Brassinosteroids also helped in acclimatization of pepper (Piper nigrum) in semi arid tropics, which is native humid region indicating the conference of habitat finless Ayurveda Inspired Drug Discovery: Promises and Challenges S. Mohan Karuppayil, Professor, School of Life Sciences (UGC SAP and DST FIST Sponsored), SRTM University (NAAC Acredited with A Grade), Nanded. MS. 431606 prof.karuppayil@gmail.com. Mobile: 09764386253 Our country is endowed with a rich biodiversity of medicinal plants. As such we are in an advantageous position for medicinal plant based drug discovery. In addition we have various codified as well as non-codified systems of medicine practiced in our country. Our traditional systems of medicine like ayurveda, siddha and unani and the systems practiced by vaidyas, shamans and tribals heavily rely on plants. Ayurveda which has its origin in Atharva Veda and has a history of at least five thousand years of use in health care. Even though ayurveda is often referred as a complimentary system of medicine in the western world, for us ayurveda is the mainstream system of medicine available to around seventy percentage of our people. There is a global re-emergence of interest in ayurveda considering its time tested efficacy against a variety of human diseases. Ayurveda has inspired many a drugs used in current day medicine. These include medicines for, diabetes, chlosterol lowering drugs, antioxidants, drugs for vitilago, cancer, arthritis to site a few. The traditional texts on ayurveda like Charaka, Susruta,Vagbhataetc. are important sources. Tremendous scope exists for pharmacological, clinical, biochemical and molecular studies to validate the claims of the drugs as per ayurveda. Many well-known drugs of ayurveda are validated and most of the time the experiments reconfirm the traditional claims. While investigating these drugs scientists have faced with a number of challenges. Since the ayurveda texts were written centuries ago in Sanskrit language one has to be careful in ascertaining the identity of the plants. It is important to understand the nomenclature dynamics of ayurveda. The language of ayurveda which is quite different from that of modern life sciences needs to be understood. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 3 The promises and the challenges faced by the scientists and present day physicians are discussed in this talk. Medicinal Plants-A Boon for Human Health Sahera Nasreen Department of Botany, P.G. and Research center, Government Institute of Science, Nipat Niranjan nagar,Caves Road,Aurangabad-431004 The conservation of medicinal plants and wealth of Vedic knowledge for the present day, clinical management medicinal plants used by ethic tribes of immigrant Muslims in the Bhramaputra valley of Assam, by people of Uttranchal, by North eastern sub Himalayan forest and Meena tribes of Jaipur district provides comprehensive account of different aspects of their utilization and conservation. Mineral nutrition of medicinal plants and treatment of Hyper lipidimea and nutraceuticals and herbal drugs are one of the important sources. The physiological active alkaloids such as morphine and codine from opium strychnine, quinine from cinchona sp. Colchine, from colchicum sps, were isolated from these medicinal plant which are very active principle saved to validate the claims of the human health as therapeutic agents is the form of drugs .These drugs are safer with no side effects as the natural products. As these medicinal plants have the active principle of the organic compounds such as terpenoids, flavonoids, carbohydrates alkaloids, lignans, fatty acids, and other organic acids etc. There are formulated generally used as water soluble salts of pure alkaloids, Gutika, or vati (dill) capsule, Arista or Asava (medicinal wine) Kwatha (decoction) Tincture (alcohol extract), Lepa (paste), Choorma (powder) and sarbat (Syrup). Thereforesome commonly available medicinal plants were studied and will be discussed with the application. Because of this the demand of such natural, holistic eco-environ friendly and herbal based therapeutics are increasing day-by-day. On the other hand there is tremendous scope of earning foreign exchange and earning in the indigenous market. The plant scientists have a role in increasing these products in the rural /forest areas by way of using their expertise Keywords- Herbal drugs, Secondary metabolites, bioactive components, therapeutics. Pharmacognostic Study of Herbal Medicine: Challenges and Opportunities Prof. S. Jeelani Director, Centre for Virtual Learning (CDVL), University of Hyderabad, Golden threshold city campus, Abids, Hyderabad Importance for the usage of alternative herbal medicine is growing every year. The WHO defined herbal medicine as comprising therapeutic practices that have been in existence for hundreds of years. Herbal medicines are in great demand in the developed countries and also in developed Nations, for primary health care not just because they are less expensive but for their cultural acceptability and compatibility with the human body. There is also a feeling that herbal medicine will have very minimal side effects, at the same time there are many challenges with the herbal drugs like herbal drugs are not being subjected to drug approval process for their safety and effectiveness. The herbal medicines are used as a dietary product in the developed Nations. The chemical constituent present in these medicines are, the part of physiological functions of the living fluorescence and are believed to have better compatibility with human body. The ancient literature also mentions that herbal medicines are very much used for age related diseases like memory loss, arthritis, diabetes, wounds, liver disorder, immunity, vitality and strengthening of the muscles. Since herbal medicines are popularly used more than 70% of world population in the developing countries, these drugs are under different process of various pharmacognistic, standardization and also vigorous method of scientific and clinical applications must be applied before they come into the market. Thus in the present paper it is discussed about the pharmacognistic International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 4 standardization techniques for undertaking the quality standards of the herbal drugs. It has also discussed on the wide opportunities available with regard to the herbal medicine not only in the day to day primary health care but for also for the communal expert at the global level. A few challenges were also discussed about the regulatory mechanism, quality standards in this paper. Key Words: - Pharmacognosy, herbal standardization. Remote sensing applications in Biodiversity and conservation C. Sudhakar Reddy* Forestry and Ecology Group, National Remote Sensing Centre, Balanagar, Hyderabad500037, India *Email: drsudhakarreddy@gmail.com Biodiversity is expressed at different levels - genetic, species and ecosystem level. Information on forest type, forest fragmentation, disturbance and biodiversity richness stands as primary source for effective conservation. Ground methods have limitations as whole area cannot be accessed in one go in many of the cases. The biodiversity needs to be surveyed, mapped, monitored and quantified. Estimates shows globally 53,000 species are medicinal. About 10,000 species of medicinal plants are endangered. 99% of medicinal plants are distributed in forests. Medicinal plant conservation may accomplish the biodiversity conservation as a whole.Species-level conservation will never meet the desired outcome, unless we conserve the ecosystems. Remote sensing, the science of obtaining information via non contact recording, has swept the fields of biodiversity, forestry, ecology and conservation. Remote sensing and Geographic Information System can be used as important tools separately or in combination for studying biodiversity at various temporal and spatial scales. The direct remote sensing approach refers to the observation of individual organisms, species assemblages or ecological communities from high spatial resolution and hyperspectral sensors. Indirect approaches rely on environmental parameters derived from remotely sensed data as proxies. Habitat parameters, such as vegetation type and forest canopy density are the primary spatial layers generated from satellite data. Using this information in conjunction with landscape spatial analysis, field data, bioclimate, preparation of different kinds of maps related to wildlife habitat, species richness, vegetation index, fragmentation, disturbance index, species composition, species vulnerability and distribution is possible. This spatial database stands as baseline forhabitat suitability assessment, prioritization for micro scale habitat studies, corridor connectivity and planning. Deforestation, biological invasion and forest fires are emerged as major biodiversity issues. Current rate of deforestation and disturbances can be monitored using multi-temporal satellite data. A geospatial approach through analysis of cumulative anthropogenic threats helps in red listing of ecosystems for conservation prioritization of biodiversity. Exploration of ‘Mode of action’ of Phytochemicals as Antioxidants and Chelating agents Dr. Y. Srinivasa Reddy, Dr. T. Manjula and Dr. B. Dinesh Kumar Food and Drug Toxicology Research Centre National Institute of Nutrition, Indian Council of Medical Research Jamai-Osmania, Tarnaka, Hyderabad-500007 Email: ysrinur@yahoo.com; Phone (O): +91 (40) 2719 7291 The constituents of traditional preparations have known various active principles. It is essential to evaluate the activity of such traditional preparations and/or phytochemicals as potential therapeutic agents. We aimed to assess i). antioxidant activity of Rasna panchaka using serum free explants culture system; ii). thiamine as heavy metal chelating agent with a battery of test. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 5 The antioxidant activity of water (4212) and water + methanol (4308) extracts (1 -10 µg/ml) of Rasna panchaka were evaluated using mouse liver ex-plant cultures. The dosage of test compound was optimized up to 24 hours followed by assessing superoxide dismutase (SOD), Catalase, reduced glutathione (GSH) and malonaldialdehyde (MDA) levels. Thiamine chelation effect and mechanism against Pb toxicity evaluated using in vitro experiments such as UV-Vis, Fluorescence spectrophotometric and solubility studies. The liver ex plant culture system suggests that the cellular architecture was well conserved up to initial 6h with gradual display of specific changes in the next 24h. There was a significant increase in MDA levels with reduced SOD and catalase levels in experimental groups indicating the oxidative stress induction in explants. Whereas, explants showed significant protection against oxidative stress when incubated with extracts at the dose of 2µg/ml. The battery of in vitro studies suggests that chelation potential of thiamine is mediated by “Pyrimidine ring”. These results concludes that Rasna panchaka is a potential antioxidant further the hydro-alcoholic extract have shown superior activity than traditional water extract. Pyrimidine ring mediated chelation might be the mechanism of ‘preventing action’ of thiamine against Pb toxicity. Pivotal Role of MedicinalPlants in Primary Healthcare of Human M. Aruna Dept. of Botany, Telangana University, Dichpally, Nizamabad -503 322, Telangana. INDIA Plants are a vital component of the World’s biodiversity and essential resource for human well-being. Besides sustenance, the plants have been used as therapeutic aid for alleviating human ailments from ancient times. Such plants commonly referred to as medicinal plants have been one of the valuable tools in the traditional system of medicine and are known to produce ingredients for formulation of new medicines in pharmaceutical industry. W.H.O (World Health Organization) has listed over 21,000 species of plants to be of medicinal use around the World. More than 60% of the World’s human population depends on medicine derived from plants. Medicinal plants play a key role in human health care from ancient period. These plants have been used over the millennia for human welfare in promotion of health. This relationship between man and environment continues even today, as large numbers of people of developing countries are still living in rural areas. WHO estimated that 80% of the people living in developing countries are almost completely dependent on traditional medicine. India is the largest producer of medicinal herbs and is called as ‘Botanical Garden’ of the World. In India traditional communities like tribal and rural people are frequently using crude extracts of local plants for medicine and other purpose. Plants consist of number of biologically active chemical compounds, which are formed during normal metabolic process. These chemicals are referred to as secondary metabolites of which there are several classes including alkaloids, flavonoids, coumarins, glycosides, polysaccharides, phenols, tannins, terpenes and terpenoids. Secondary metabolites are known to exhibit numerous biological activities that promote health effects. Medicinal value of plants is due to the presence of a substance or substances present in its tissue which produces a physiological action on the body. Most important of them are alkaloids, compounds of carbon, nitrogen, oxygen and hydrogen. Glucosides, essential oils, fatty oils, resins, mucilages, tannins and gums are all utilized. Some of these are poisonous; hence the preparation and administering of them should be entirely supervised by physicians. Based on the habit, plants can be classified as trees, shrubs and herbs. Different parts of the plant either as a whole plant, roots, stem, bark, leaves, fruits, bark flowers and seeds may possess medicinal value and is administered either in the form of a tablet, grinded as powder or paste and is used for treatment of numerous diseases such as diarrhoea, dysentery, skin International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 6 infections, rheumatoid arthritis, hypertension, insomnia, jaundice, fever, cold, menstrual pain, uterine disorder, ulcer, hyperglycemia, leukaemia and cancer Knowledge of usage of traditional medicine by ethnic communities is not only useful for conservation of culture, traditional values but also play an important role in health care of community and drug development in present and future. A floristic survey of Botanical garden of Telangana University campus was carried out during 2014-2015 and plants of medicinal importance were identified and labelled. In the present work it is intended to discuss the medicinal properties of plants enlisted along with their applications in ailment of various diseases in human. Next-Generation RNA Sequencing for Gene Discovery in Medicinally Important Plants Praveen Mamidala Department of Biotechnology, Telangana University, Nizamabad, Telangana – 503322 E-mail: pmamidala@gmail.com Plants are an abundant source of medicinal compounds and till date research on plant-derived medicinal products has traditionally been led by chemists and phytochemists. However, as the association between secondary metabolites and genes those encode proteins involved in their production remains elusive. Medicinal plants, which produce different classes of natural products, cover a wide range of plant taxa and most of them have only limited transcriptomics and/or genomic data. Transcriptomics (complete snapshot of all the transcripts at a given time) in a particular tissue or organism has been transformed with the introduction of next generation sequencing (NGS) platforms such as 454 pyrosequencing (Roche Diagnostics), Illumina Solexa sequencing (Illumina, Inc.), SoLiD (Applied Biosystems), etc. Particularly in non-model organisms, NGS has revolutionized genomic research through RNA sequencing, wherein little to no molecular knowledge exists. The molecular resources (expressed sequence tags, ESTs) developed from these NGS platforms allow to identify novel genes and quantify rare transcripts without prior knowledge of a particular gene. Further, NGS platforms facilitate single nucleotide polymorphism discovery (molecular markers), mutation mapping, identifying transcripts resulting from alternative splicing, etc. Very particularly, the organ-specific gene expression using NGS platforms have made us to better understand in vivo biological processes and the unprecedented quantity of transcriptomic data has enabled biologists to examine several genes involved in secondary metabolism of plants. Keywords: RNA Sequencing; Next Generation Sequencing Platforms; Pyrosequencing; Illumina; Transcriptome. Medicinal Plants for Better Human Health: Social Work Interventions K. Rajeshwari Department of Social Work, Telangana University, South Campus, Bhiknur, Nizamabad.drkrsocialworktu@gmail.com Key Words: Medicinal Plants, Human Health, KAP-Knowledge, Attitude and Practice and Social Work Interventions. Health is a state of complete physical, mental and social well-being and not merely the absence of disease or infirmity (WHO, 1946). The Definition has not been amended since 1948. Medicinal plant is any plant whose roots, leaves, seeds, bark, or plant part is used for therapeutic, tonic, purgative, or other health-promoting purposes. Health is a resource for everyday life, not the object of living, and is a positive concept emphasizing social and personal resources as well as physical capabilities. Health is a fundamental human right, recognized in the Universal Declaration of Human Rights (1948). It is also an essential component of development, vital to a nation's economic growth and internal stability. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 7 Modern day stressful life-style has led to a rise in life-style diseases all over the world. It has been realized that no single system of medicine can address the health care needs of modern society. Around 65-70% of the population in rural India use traditional system of medicine. One of the Goals of National Health Policy 2002 in India is to increase access to tried and tested systems of traditional medicine. India has rich tradition of medicinal wisdom coupled with its strong capability in modern medicine. The Ministry of AYUSH was formed with effect from 9 November, 2014. The Department of Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy, abbreviated as AYUSH, is a governmental body in India with and aim of developing education and research in (Indian traditional medicine). Ayush is also a common Hindu given name, derived from Sanskrit, meaning "Long-life". Long life with Complementary and Alternative Medicine. Medicinal Plants is one such Alternative Medicine. In order to achieve the Millennium Development Goals of 2015 and National Health Policy goals in India, it is important to know about the Social Work Interventions. Social Work Interventions like Social Case Work, Social Group Work, Community Organisation, Social Work Research, Social Work Administration and Social Action help in improving the Knowledge, Attitude and Practices (KAP) towards use of Medicinal Plants for Better Human Health. It is the Professional Social Worker who has to study the (KAP) regarding Medicinal Plants and also (KAP) on Better Human Health. It is an integrated approach of a Plant Scientist and Social Scientist. STEVIA: The alternative for sugar A. Ravi Charan*, V. Prathap Reddy and Ranjit Nagireddy PRR Biotech Innovations Pvt. Ltd. Stevia is the only internationally recognized zero calorie natural high sweeteners with Medicinal and Nutritional properties. It is well known that Air, Water, Food and Stress are the basic sources for the multiplication of harmful bacteria in the Human body leading to almost all the Diseases. Going back to 327 BC, the people in India used raw sugar as sweetener. Since then, humans have acquired taste for sugar. Sugars are carbohydrates and are the foundation of our food chain. Sugars includes: Glucose (dextrose or blood sugars), Fructose (levulose or fruit sugars), Galactose (occurs only in milk) (1, 2). Our body becomes addicted to the sweetness but it has become a danger to our bodies, sugar can “attack” our white blood cells which are our bodies defense system against Diseases. The white blood cells are formerly known as Phagocytes. Two teaspoons of sugar can reduce Phagocytes strength by 25%. Intake of large portions of sugar can make our Immune system 100% helpless. We call this as “sugar rush”. This amount of sugar intake can be a trap for a cold, Diabetes, Obesity, Arthritis, damage of collagen (cause wrinkles), sometimes even cancer (4). A positive outlook on a sugar substitutes are that more natural forms of sugar substitute are now available instead of package of artificial sweeteners. A product that is well on its way to become a popular sweetener on the market is Stevia. Stevia is an ideal sweetener in place of cane sugar because of natural sweetener and specifically for those in pursuit of Health. Stevia is approved by World Health Organization (WHO), World Food and Agricultural Organization (FAO) (3) and USA Food and Drug Authority (FDA) (5). Stevia leaves contains a number of important Nutrients and Flavonoids that are lacking in many of the foods we eat which are vital to various glands and organs of the body to function correctly, with precision and balance. It can be used as a sweetener in various preparations i.e. hot and cold beverages, food, bakery, sweets, ice creams, energy drinks etc. Stevia is the only safest alternative and essential for Diabetes and widely proven for Obesity, Constipation, Mouth diseases, Heart, Blood pressure, Dental/Gums, Skin/Allergies/Psoriasis, Hair care, Cold/viral, Asthma, Women complaints, Tumors/Injuries, Arthritis, Rheumatism, International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 8 Cancers, Urinary /Kidney infections (6, 7). We have developed some techniques in Stevia cultivation by using natural organic materials. Studies on Grass diversity of Karaikal District, U.T of Puducherry K. Sambandan, N. Dhatchanamoorthy and S. Kalaivani Department of Plant Science, Avvaiyar Govt College for Women, Karaikal, U.T of Puducherry Email: sambandhan@gmail.com The grass family Poaceae is one of the most important families to mankind. Grasses occur in virtually every terrestrial habitat, cover as much as one-fifth of the Earth’s land surface. The cereals, sugarcane, bamboos and forage and weedy grasses are of great importance in human economy. They are directly consumed as food by human beings or indirectly through the animal products. The grass family includes approximately 11,000 species in over 700 genera at global level. India is having 240 genera and 1,200 species of grasses. The present work was carried out the exploration and documentation of species composition of grasses and its distribution in the Karaikal district. In the present study, Sixty two grass plant species belonging to 42 genera were recorded during the study period. Genus wise analysis of Poaceae shows Eragrostis with 5 species followed by Aristida, Brachiaria and Panicum with three species each and Chloris, Cynodon, Digitaria, Echinochloa, Ichaemum, Leptochola, Paspalidium, Paspalum and Sporobolus with two species each. Twenty eight genera of Poaceae are represented by single species in the present study. Most of the genera represented in this study are mainly tropical in distribution. Twenty five grasses are flowering and fruiting period extended throughout the year followed by 21 grass species flowering and fruiting only in winter and rainy months where as 15 grass species fruiting and flowering fall only during summer months. Among the 60 grass plant species, 53 plant species are commonly available in the district whereas 4 plant species are less common and 5 plants are rarely available in the district. The anthropogenic activities like over-grazing, overexploitation of natural resources, human habitation, invasion of alien species, urbanization and industrialization activities were highly threatening the valuable grasses in the district. There is an immediate need of conservation activities to preserve the valuable grass genetic resources for sustainable utilization for the future generation. Role of Endophyitic actinomycetes from Medicinal Plants in Dharwad region Waheeda Khanam and Vootla Shyam Kumar Post Graduate Department of Biotechnology and Microbiology, Karnataka University, Dharwad-580003, Karnataka Endophytic microorganisms and plants have developed good information transfer, leading to the hypothesis that plants with an ethanobotanical history are more potent sources of endophytes producing active natural products than other plants. Endophytes are microorganisms that, for the whole part of their life history, live inside plant tissues without causing immediate overt negative effects as a result of these long hand associations. Medicinal plants are a precious source of endophytic microbes which possess abundant biological and chemical diversity. These endophytes not only make the same bioactive compounds originally from their host plants but also have the ability to produce more novel chemicals with potent bioactivities. They have become a rich source of natural products, which some of them could be used as new drug candidates and agrochemicals. Therefore, it is very important to pay more attention on the studies of biology and chemistry of endophytes from medicinal plants. Actinomycetes are the main source of antibiotics and endophytic actinomycetes isolated from medicinal plants has considerable development potential. From the present findings, some new actinomycetes from tissue of medicinal plants have been founded constantly. Moreover, most endophytic actinomycetes of medicinal plants can produce important compounds and some of them are new chemical structure. The scope for International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 9 screening new endophytic microorganisms among myriads of plant ecosystems is very much appealing. Endophytic actinomycetes from ethnobotanical plant Ocimum basilicum in Karnatak University, Dharwad were isolated. Medicinal plants are of significant interest as the producer of important bioactive compounds by endophytic microorganisms. A novel method of surface sterilization and standardization of media for isolating endophytic actinomycetes from Ocimum basilicum yielded 32 isolates of which 10 isolates were identified based on their morphological and phenotypical characters. Starch casein agar amongst the media and surface sterilization method using calcium hypochlorite, sodium azide and sodium hydrogen carbonate was proved to be effective for surface sterilization. Bio-Plastics a Long Way to Solve Environment Pollution A. Dharani Dept. of Library Science, GDC (W), Srikalahasthi Now-a-days plastic is more common in human life, but it shows negative effect on humans and wildlife and it takes more and more time to degrade. Industrial growth, urbanization, wrong agricultural practices etc., are responsible for pollution and loss of environmental quality. Nature's built-in mechanisms and self-regulation ability cannot tackle novel pollutants since nature is unfamiliar to it. Phthalates, Bisphenol-A are the toxic compounds released from plastic waste and over dose of these compounds may cause ulcers, cancer and also shows effect on reproductive system in humans. We will have to remain in tune with the nature and exploit its balancing capacities to ultimately make our environment comfortable. Bio-plastics are one such reality which can help us to overcome the problem of pollution caused by plastics. It can be used for manufacture of variety of containers, articles and packaging material which otherwise are produced by using non-degradable plastics. There is a lot of research going on to develop bioplastics which can economically replace conventional plastics. Efficiency of AgNPs in combined mode with drugs against pathogenic bacterial strains Anima Nanda FNRS Faculty of Bio & Chemical Engineering, Sathyabama University Rajiv Gandhi Salai, Chennai-600119, India Development of nanomedicine to fight against drug resistant bacteria, the present study was an attempt to carry out a comparative analysis of the extracellular biosynthesis of silver nanoparticles from different Penicillium species obtained from wild and MTCC cultures and was processed for their antibiotic efficacy on its own and with other antibiotics against pathogenic clinical bacteria. Characterization by UV- spectrophotometer showed that the nanoparticles have the absorption peaks in between 410- 439nm, which are specific for the silver nanoparticles. Fourier Transform Infrared Spectroscopy (FTIR) analysis showed the presence of proteins, molecules and functional groups in the nanoparticles formed, which were found responsible for the stabilization of the nanoparticles as capping agents. Field Emission Electron Microscopy (FESEM) confirmed the nanoparticles are spherical and are well dispersed. Atomic Force Microscopy (AFM) analysis showed the agglomeration of nanoparticles and determined the average roughness and confirmed the data of FESEM. XRD analysis showed the metallic nature of the nanoparticles and their face was cubic centered. Silver nanoparticles synthesized from all the ten Penicillium spp were checked for their antibacterial activity against clinically isolated bacterial pathogens and found perfection. The efficiency of drugs was remarkably enhanced with their antibacterial properties when combined with silver nanoparticles against the bacteria studied. P. glabrum wasalso found with good anticancer activity against A-549 lung cancer cell line during our present study. Key words: Nanomedicine, AgNPs, AFM, FESEM, XRD, Wild and MTCC cultures International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 10 Traditional knowledge for Modern Medicine K. Prasanna Rani* Dept. of Law, Telangana University, Dichpally, Nizamabad. “When an elder dies a Library Burns” This statement is ever truth for all subjects irrespective of Race or Religion, Caste or Complexion and Place and Philosophy. It is a fact that for time immemorial people have healed with herbal or animal derived remedies, using knowledge handed down through generations. In Africa, Asia, Latin America and the Middle East, 70-95 per cent of the population still use traditional medicine (TM) for primary healthcare. And some 100 million people are believed to use traditional, complementary or herbal medicine in the European Union (EU) alone — as high as 90 per cent of the population in some countries. 1 The industry is worth big money. In 2012, global sales of Chinese herbal medicine reached US$83 billion, up more than 20 per cent from 20112. The global market for all herbal supplements and remedies could reach US$115 billion by 2020, with Europe the largest and the Asia-Pacific the fastest growing markets. The demand is driven by women as the main consumers of dietary supplements, by growing emphasis on healthy living and concerns over the side-effects of mainstream drugs3. Meanwhile it is not out of context to note that modern medicine has desperate side effects. More over new drugs are costly as it has to get through the research and development pipe line. And use of several antibiotics is leading to raise drug resistance. As such most of the science fraternity is realizing the importance of traditional knowledge for modern medicine. Excavations at different sites suggest that medical interventions such as dentistry and trepanation were practiced as early as 7000 BCE in the Indian subcontinent. Organized forms of agriculture practiced by the people of the Indus civilization, the importance they gave to certain medicinal plants and trees and the emphasis on hygiene and water sanitation suggest an advanced awareness of health management. Trade routes linked the Indus valley civilization to other parts of the subcontinent and westward to Persia, Mesopotamia and the Arabian Sea, and northward to Central Asia. It is highly likely that botanical and medical commodities and knowledge were among the prized items of exchange. Recent archaeobotanical excavations give evidence for the use in the Middle Gangetic region of medicinal plants since the 2nd millennium BCE that are still used by Ayurvedic physicians and folk healers. In this context this paper aims to focus mainly on three aspects. First aspect dealing with main problems in modern medicine and Second aspect reveals Indian Traditional Knowledge about human health and finally third aspect concentrates on legal implications in using traditional knowledge for modern medicine with possible suggestions. Biosynthesis Silver Nanoparticles from Syzygium aramaticum and Their Antimicrobial Activity Siddiqui A G, Awasthi R S Dept. of Microbiology and Biotechnology, Dayanand Science College, Latur, Principal, Shivaji College, Renapur, Dist: Latur Metal nanoparticles (1-100 nm) are being used in different fields including electrical, biological, textile, and chemistry. Silver nanoparticles due to their antimicrobial activity have wide applications in medical field; therefore eco-friendly and nontoxic process of synthesis of nanoparticles is of great importance. Hence we attempted to synthesize silver nanoparticles from Szygium aramaticum, preliminary confirmation was done by UV spectrum and checked 1 Molly Meri Robinson and Xiaorui Zhang The world medicines situation 2011 (WHO, 2011) WHO traditional medicine strategy 2014-2023. (WHO, 2013) 3 Ibid. 2 International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 11 their antimicrobial activity against some microorganisms. We got optimistic results from this study. Key Words: Silver nanoparticles, biosynthesis, antimicrobial activity Standardization of Avarttani (Helicteres isora Linn. Stem bark)-An Ayurvedic drug J. Shashikanth, P. Ramachandra Reddy* Plant Anatomy and Taxonomy Laboratory, Department of Botany, University College for Women, Koti, Osmania University, Hyderabad-500007. *Corresponding author’s E mail:jale_shashikanth@yahoo.com Helicteres isora L. popularly known as‘Avarttani’ belongs to Sterculiaceae family. The stem bark is extensively used in Indian system of medicine. The stem bark is useful in skin diseases, dysentery, piles and diabetes. Standardization and quality control of herbal drugs used in Ayurveda is essential for their acceptance in the international as well as local markets. Thus, there is urgent need of standardized drugs having consistent quality for reliable beneficial therapeutic purpose. Physicochemical parameters, qualitative analysis, histochemical studies, powder treating with different chemical reagents, Hptlc profile and fluorescence analysis were used for standardization and quality evaluation of ‘Avarttani’. Preliminary phytochemical screening reveals presence of alkaloids, phenolic compounds and tannins, saponins, flavonoids and carbohydrates. Key words: Standardization, Helicteres isora L., Ayurveda, Preliminary phytochemical screening, Physicochemical, stem bark Evaluation of Regeneration Potential of Mature Seed Culture in an Anti-Diabetic Herbal Legume Crop- Trigonella foenum-graecum L. Vikrant* and Karthiga, K. Post-Graduate Department of Plant Science, Avvaiyar Government College for Women, Karaikal- 609 602, U.T. of Puducherry, India. *Corresponding Author and Present Address Post-Graduate Department of Plant Science, Mahatma Gandhi Government Arts College, New Mahe- 673 311, U. T. of Puducherry, India. E.mail: dr.s.vikrant@gmail.com In the direction to minimize or to control diabetes to some extent, medicinal plants as such and their products have been highly effective. Therefore, establishment of a high efficiency in vitro regeneration system is a prerequisite for gene engineering based medicinal plant improvement. Mature seeds of the most promising medicinal crop Trigonella foenumgraecum L. (family Leguminaceae) were undertaken in present study. The combination of cytokinin and auxin, particularly the concentration of cytokinin, in the regeneration medium has been proved as most critical factor to affect the regeneration potential of explants. As for the different hormonal treatments, (BAP 5 mg/L + 2,4-D 1.5 mg/L) combination was found to be the best treatment, followed by (BAP 2.5 mg/L + 2,4-D 1.5 mg/L) in terms of frequency of shoot-buds regeneration from induced callusfrom the cultured explants. During this study, BAP alone at high concentration (5 mg/L) supported shoot-buds regeneration but frequency was relatively very poor whereas, 2,4-D alone in the nutrient medium failed to show morphogenic response and mature seeds merely exhibited slow germination followed by slight swellings at the basal region of the seedling. These results based on investigation of regeneration potential established for medicinal plant Trigonella can be applied to obtain at large scale or for genetic transformation studies. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 12 In vivo anti-inflammatory effect of various extracts of Canthium coromandelicum (Burm. F.) Alston Priyadarshini S. Ekambe* and B. S. Surwase School of Life Sciences, S. R. T. M. University, Nanded- 431606, Maharashtra, India. Aim: The research work was carried out to investigate presence of phytochemicals and to evaluate anti-inflammatory activity of methanol, acetone and ethanol extracts of Canthiumcoromandelicum(Burm. f.) Alston. Materials and Methods: The extract of Canthium coromandelicum leaves were prepared by maceration method in different organic solvents as 70% methanol, 70% acetone, 70% ethanol. All extracts were analyzed for presence of phytoconstituents like alkaloids, glycosides, tannins, saponins, proteins, sterols, phenolic compounds, resins, reducing sugars, flavonoids and anthraquinones. The anti-inflammatory activity of all extracts was screened by carrageenan induced paw edema model in Wistar rats. The extracts were administered (@ 500 mg/kg body weight) and the obtained effects were compared with control. Results: The phytochemical analysis of all extracts revealed presence of alkaloids, glycosides, tannins, sterols, phenolic compounds, reducing sugars and flavonoids. Anthraquinones were present only in ethanol extract, whereas, a phytochemical saponin was absent in acetone extract. Anti-inflammatory activity of methanol and acetone extracts was statistically significant (P < 0.05) and was shown by decrease in paw edema. Ethanol extract also reduced paw edema but did not show significant difference. Conclusion: The significant anti-inflammatory activity of methanol and acetone extracts of Canthiumcoromandelicum (Burm. f.) Alston leaves may be due to the presence of glycosides, alkaloids, tannins, saponins, sterols, phenolic compounds, reducing sugars, flavonoids. Keywords: Phytoconstituents, Carrageenan, Anti-inflammation. Evaluation of Antioxidant Activity in different parts of Costus igneus(L.,) Elizabeth Margaret*, Dr (Mrs.) Kalpana & Ms. Roja Department of Botany, St. Ann’s College for Women, Mehdipatnam, Hyderabad. Email id: margaret_nje@yahoo.com Recently there is upsurge of interest in therapeutic potential of plants, as antioxidants, reducing free radical induced tissue injury. Cancer, cardiovascular disease, diabetes, age related muscular degeneration implicate Oxidative stress. Such Diabetes has serious consequences and limited treatment. Antioxidants prevent oxidative damage caused by free radicals interfering with oxidative process, chelating, catalytic metals and as oxygen scavengers. Present study focuses on antioxidant activity evaluation of Costus igneus (L.) (insulin plant), of family Costaceae – efficient, effective herbal drug possessing various pharmacological activities - hypolipidemic, diuretic, antidiabetic, antioxidant, antimicrobial, anticancerous. Pharmacological work was carried out on Costus igneus leaves but pharmacological potential of other plant parts requires exploration. The present study aims at evaluation of antioxidant activity in methanolic extract of leaves, rhizome, and stem. An attempt is made to find correlation between phenolic, flavonoid content and antioxidant activity. Examining the exact mechanism and phytoconstituents responsible for the observed effects warrants further study. Key Words: Costus, Insulin plant, Antidiabetic activity, Pharmalogical, Phytochemical International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 13 Isolation and Characterization of Anti-Alzheimer’s Compounds from Celastrus paniculatus B. Nirmala Kumari and N.Sudhakar 1.Department of Zoology, GDC (W), Srikalahasthi, Andhra Pradesh, India 2. Department of Microbiology, GDC (W), Srikalahasthi, Andhra Pradesh, India For Correspondence: nirmalakumarib@gmail.com Alzheimer’s is the most common form of dementia. It is an age-related neurodegenerative disease characterized by progressive memory loss followed by general cognitive decline affecting older persons. Ayurveda is the knowledge of healthy living and is not merely confined to the treatment of diseases or disorders. Celastrus paniculatus have been used in the ancient systems of medicine for the treatment of various neurological disorders. The main objective of the present research work is to isolate and characterize antiAlzheimer’s compounds from selected medicinal plants and to define their neuroprotective activity against neurological disorders in general and AD in particular. Male Wistar albino mice of 30 days age, weighing 20±2 grams will be use as the experimental animal model in the present investigation. Separation of solvent extracts from crude extract and isolation of bioactive compounds from the selected plants will be carried out by employing the appropriate assay techniques such as Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR). The effective dose for different fractions of these selected plants will be determined. The performance skills of the control and experimental mice will be determined through Morris Water maze experiments. Biochemical estimations on the Cholinergic System, Biogenic amines, Glutamate metabolism and ATPases neurotransmitters and enzymes will be done in different regions of brain (Cerebellum, Hippocampus, Cerebral Cortex and Ponsmedulla) in vivo as per the experimental schedule in Control and Experimental mice. After thorough analysis of the experimental results on the above mentioned experimental parameters, the nature and mode of action of these different fractions of the medicinal plants will be assessed. From these findings, the therapeutic compounds having anti-Alzheimer’s properties will be isolated and characterized through appropriate techniques. Detection and Identification of Pesticides in Fruits and Vegetables Syeda Azeem Unnisa Department of Environmental Science, University College of Science, Osmania University, Hyderabad Telangana State, India E-mail:syeda_30@yahoo.co.in The present study deals with the detection and identification of pesticides residues in fruits and vegetables collected in and around Hyderabad. Randomly 40 samples were collected from the market and tested for the pesticide residues by finger printing technique where by visual color reaction of pesticides indicates the presence of residues. The results reveal that 60 percent of fruit samples and 80 percent of vegetable samples showed presence of pesticide residues of HCH, DDT, Carbaryl, Endosulfan, Carbofuron and Aldrin. The presence of pesticides residues indicate that it is applied on harvested fruits and vegetables directly for control of pests and diseases during production of crops. The problems of consumer safety, quality of the products and legal tolerance of the compound produce become important. The study recommends that the usage of organic pesticide should be promoted and awareness should be brought in the agriculture sector specially taking farmers into consideration. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 14 Herbal medicine: Parkinson's disease G. Swathi Dept. of Zoology, Government Degree & PG College, Srikalahasthi Parkinson’s disease, a chronic, degenerative disorder of the nervous system, results from dopamine-producing brain cell deficiency. In recent days phytomedicine is gaining due to its less side effects, Herbal medicines have attracted considerable attention in recent years, which are used to treat Parkinson's disease (PD) in modern pharmacological theories. We summarized and analyzed the anti-Parkinsonian activities of herbal medicines and herbal formulations investigated in PD models and provide future references for basic and clinical investigations. All the herbal medicines and herbal formulations were tested on PD models in vitro and in vivo. The relevant compounds and herbal extracts with anti-Parkinsonian activities were included and analyzed according to their genera or pharmacological activities. In this article we had analysed two herbal crude extracts which showed positive effect and acted as antiparkinsonian drugs.These herbal medicines can be an alternative and valuable source for antiParkinsonian drug discovery. The plant species in these genera and families may be the most promising candidates for further investigation and deserve further consideration in clinical trials. Active components in some of the herbal extracts and the compatibility law of herbal formulations remain to be further investigated. Key words: Parkinson's disease, Herbal medicine, antiparkinsonian drug. Estimation of E&Z-guggulsterones and In vitro Propagation studies inCommiphora wightii (Arn.) Bhandari Ch. Mohan, D. Sreekanth, P. Manjula, B. Keerthi, B. Kiran Kumar and Prathibha Devi* Plant Biotechnology and Molecular Genetics Laboratory, Center for Advance Studies in Botany, Department of Botany,Osmania University, Hyderabad-500007, India. Corresponding author E-mail address: pathi56@yahoo.com The qualitative analysis of Commiphora wightii (Arn.) Bhandari showed the presence of various phytochemicals like flavonoids, steroids, tannins, saponins, terphenoids, phenols and glycosides. The gum guggul-resin extract of the plant has the cholesterol-reducing activity of the guggul-resin well documented in the Ayurveda. Quantitative analysis for total steroids produced interesting results. The E-Guggulsterone and Z-Guggulsterone are steroid compounds reportedly present in the Gum of Commiphora wightii plant. A high-performance liquid chromatographic method has been developed presently in a DST -PURSE project and validated for the quantitative determination of E- and Z-guggulsterones, the hypolipidemic agents in the gum-resin exudate of Commiphora wightii, currently marketed worldwide as hypocholesterolemic. The method involves extraction of the guggul-resin from the raw exudate and chromatography on a Zorbex SB C-18 column using (mobile phase A)-Ortho Phosphoric acid in water and acetonitrile-water gradient. The method C18 has a validated quantitation range of 2.45 mg/l with 9.279 minutes retention time at 243 nm for Eguggulsterone and 2.17 mg/l with 11.495 minutes retention time at 243 nm for Zguggulsterone in Commiphora wightii crude resin extracts and final products.In vitro propagation studies has been taken up with nodal explants by using different concentrations and combinations of growth regulators and micropropagation protocol have been developed. The regenerated plantlets were successfully transferred to the glasshouse, acclimatized and transferred to the field. Keywords: Commiphora wightii, Guggul-gum (resins), Qualitative and Quantitaive analysis, HPLC, E-and Z-guggulsterones, In vitro propagation International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 15 Traditional knowledge on Indian herbal Medicine S.Padmavathi, Department of Botany, GDC, Nagari. India has a wealth of biological and abundant natural resources in its tropical forests in the form of medicinal plants. It also has a rich heritage of traditional medicinal knowledge which is derived from multiple medicinal traditions like Ayurveda, Siddha, Unani, Homeopathy and which is passed on through generations. The traditional knowledge of the local community needs to be collected and preserved. This traditional medicine or Ethno Medicine is perceived as efficient, safe, affordable and accessible. In some Asian and African countries 80% of the population relies on the traditional medicine for their primary health care needs. The commercial use of the herbal medicine has been growing in the recent years. However, many of them were not processed through an appropriate production activities and technology, which doesn’t lead to consistent quality and significant value to the economy as well. This article would provide ways in which commercial use of medicinal plants as well a s traditional knowledge can be very perspective in the future. The modernization policy also includes efforts to encourage scientific research, clinical trials to document the effectiveness and safety of traditional medicines. Airborne microfungi in rural environments of Puducherry region Bijaya Kumar Nayak Department of Botany, K. M. Centre for Post Graduate Studies (Autonomous), Lawspet, Airport Road, Pondicherry-605008, India Diversity of fungi are ubiquitous in nature, they occur in wide spectacular array of shapes, sizes and colors. Dispersion of microfungi is unique and their distribution varied from each environment and constitutes the major portion of the suspended bioparticulate matter in the air. It is exceptionally important to note that vast majority of fungal diversity are given least importance and remains ever unexplored. There is no aerobiological work has been made in rural environments in India. In our recent study, volumetric analysis of airborne microfungi of rural environments of Puducherry region was carried out by employing Burkard’s personal sampler on SDA mediated petridishes. Air samplings were made for one year at fifteen day intervals in order to isolate the prevalent fungi from the study sites at diurnal time intervals viz., morning noon and evening. Altogether, more than seventy fungal species under fifty genera were isolated, among which Aspergillus spp were recorded as the dominant followed by Penicillium spp. The incidence of fungal spores was found more in the areas of high vegetation in comparison to low vegetation localities. Diurnal variation showed that, noon was found to harbor more number of spores in composition and concentration than morning and evening. Seasonal incidence of fungal spores was quite regular in our study, and showed the trend more in winter followed by summer and rainy seasons. In addition to aspergilli and penicilli, other fungi like Alternaria, Cladosporium, Curvularia, Fusarium, Monascus, Mucor, Paecilomyces, Rhizopus, Trichoderma and Verticillium were also observed from the localities. The fungal spores viz., aspergilli and penicilli as well as Alternaria and Cladosporium were found to be allergenic predominant, probably due to their wide host range, substrate adaptability and opportunistic nature. It was observed that the variation of the fungi was highly correlated to the substrate precipitation needed for the fungal growth and the local vegetation. Keywords: Airborne microfungi, Burkard’s volumetric air sampler, SDA, CFUs. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 16 Evaluation of Free Radical Scavenging Potential and Anti-cataract Activities of Selected Medicinal Plants 1 Dhole N. A.*, 2 Gacche R. N. 1 Digambarrao Bindu Arts, Commerce & Science College, Bhokar, Nanded-431801 2 School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded-431606 (Corresponding author: nageshdhole@gmail.com ) Diabetes mellitus is a metabolic syndrome which affects more peoples in all developing as well as in developed countries. The rate of prevalence of diabetes and its secondary complications is more in Asian countries like India and hence “India is considered as Diabetes capital of the world”. Phytomedicine has significant role for the prevention and management of diabetes and its secondary complications. The present study describes the evaluation of free radical scavenging potential and anti-cataract activities of different fractions of Gymnema sylvestre, Boerhavia diffusa, Cassia occidentalis,Cyathocline purpureaand Annona squamosa. The antioxidant properties were evaluated by determining DPPH (1, 1-diphenyl-2-picrylhydrazyl), FICA (Ferrous ion chelating ability), RP (reducing power) and H2 O2 (hydrogen peroxide) scavenging activity. The water fraction (IC 50, 262 µg/ml) of Gymnema sylvestre and ethanol fraction (IC50 , 300 µg/ml) of Boerhavia diffusa showed maximum DPPH radical scavenging activity while other fractions of selected plant exhibited considerable antioxidant potential. Sugar induced lens opacity studies revealed that, water fractions of Cyathocline purpurea, Gymnema sylvestre, and Boerhavia diffusa possess significant anti-cataract potential as compared to glucose induced lens opacity in goat lens model. Keywords: Diabetes, Antioxidant, Anti-cataract, Medicinal plants DNA – Welfare of Living Beings Madhamanchi Pradeep,Department of Biotechnology, Govt. Degree College (Men), Srikakulam, Mail: mpsep15@gmail.com Advancement of Life Sciences in all aspects is mainly due to better understanding of Central Dogma of Molecular Biology. The versatile and keystone molecule of Central Dogma is DNA that promotes almost all biological processes directly or indirectly in living organisms. So better understanding of DNA make possible to manipulate it several ways and improve quality and quantity of lives through Biotechnology Key words: Life Sciences, Biology, DNA, Biotechnology, Central Dogma KatiraGond- Novel Natural Product Srinivasa Reddy CH1, N V S S Satyadev T2 , Manoranjani M 2 1.Department of Botany, PB.Siddhartha College of A & S, Vijayawada 2.Department of Chemistry, P G Centre, PB.Siddhartha College of A & S, Vijayawada Tragacanth (KatiraGond) gum comes from a thorny shrub. It is a tree gum like myrrh, frankincense and balm of Gilead and can be used, along with the stems of the plant as incense. It comes from a number of Astragalus species, but the one which produces the best quality gum is Astragalusgummifer.The gum exudates naturally from the roots of plant and from incisions made in the stem. This is collected and when dried forms crystals of flakes or may be formed into blocks. The plant is member of Fabaceae. Externally the gum has been used in traditional medicine for dressing burns and it is now believed that it might have antitumor activity and could stimulate the immune system.Tragacanth gum has been used as laxative, antitussive, anti-diarrhoeic, and as an aphrodisiac. It is used as an adhesive agent in making pills and tablets, as an emulsifying agent in lotions, creams and pastes and as a thickener in cosmetics, toothpastes, jellies, salad dressings, syrups, mayonnaise, sauces, liqueurs, candy, ice cream and popsicles. It has both cooling and heat producing effects. In summer after soaking in water it becomes fluffy and soft. Consuming it in this form in International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 17 summer helps to cool our body. In winter we use it after frying and grinding. It can be added to many sweets. And then it generates heat in the body. It has the qualities of laxative and so it helps in constipation. It helps to cure weakness in men. In women it helps to increase breast size. Key Words: KatiraGond, Gum Exudate, Astragalus Management of Fusarium Wilt by Botanical pesticides in Chilies- an important commercial cash crop E.Shravya puri*, A. Sabitha Rani Department of Botany, Osmania University College for Women, Koti, Hyderabad. In recent years, many plant species are being exploited for production of green pesticides, which are effective and ecofriendly. Chillies (Capsicum annum), one of the important cash crop is susceptible to various types of fungal diseases. Hence, the present study has taken up to evaluate plant extract against Fusarium oxysporum, pathogen of wilt disease of chillies. The methonolic extract of Butea monosperma (Family-Fabaceae)leaf was evaluated in vitro for its antifungal activity. Among various fungal pathogens, high inhibition zone were observed against Fusarium oxysporum (26mm), Colletotrichum (22mm) followed by Fusarium moniliformae (19mm). Moderate activity was noticed in Rhizoctonia (18mm) Macrofomina (17mm) and Alternaria (16mm). The leaf extract was also evaluated in vivo on experimental plants established in the pots. Different concentration of plant extract (10, 20, 30 and 40 %). was sprayed at various time intervals, staring from 15 days of sowing to flowering and fruiting stage. The biological efficacy was determined by percentage control of disease incidence, which ranged from 20 to 60% among different treatments of plant extract. Data from the field trials of second cycle i.e. the present season is under study. The study proves that plant based formulation can be recommended as alternative protection agents for vegetables crops against fungal diseases. Micropropagation Studies on Stem Node Explants of Cucumis sativus L. A Medicinal Important Plant M. Venkateshwarlu Department of Botany, Kakatiya University, Warangal – 506009 T.S. (India) E-Mail Id: drvenkat6666@gmail.com Plant tissue culture methods were applied for micropropagation of this medicinal important plant. A reliable regeneration system culminating in the formation of complete from the mature stem nodes segments having 1 – 2 nodes. After initial swelling a maximum of 6 – 10 shoot buds were obtained on MS medium supplemented with a combination of Kn and BAP moreover like Adenine sulphate, ascorbic acid. Citric acid and glutamine were also found to be beneficial when incorporated in the above mentioned medium sholls was obtained on a separate media comprising of half the concentration of M.S. inorganic salts and high concentration of IAA plantlets thus obtained were hardened acclimatized and finally transferred successfully to field conditions. The study established reliable and reproducible protocol for rapid multiple shoot induction from stem node explants ofCucumis sativus L. using different concentration and combination of cytokinins. The present study was to develop a rapid system for regenerating shoots from mature stem node explants ofCucumis sativus L. These plants are propagated through cutting process but are a labour intensive and time consuming process. The percentage of explants exhibiting shoot induction was found to be between 50-60 i. most of the concentrations of Benzyl amino purine. Several workers in past have micro propagated. Some of the important Asclepiadaceae members such as Ceroegia bulbosa (Britto et al, 2003).Murashige and Skoog (1962) medium supplemented with 0.5 to 2.0 mg/l BAP was found to be optimum to induce shoots directly from the stem node explants. Since very scarce information is available about micro propagation of this International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 18 important vegetable crop plant, an attempt was made to develop a reproducible protocol for multiple shoot induction form stem node explants of one the culture. Free radicals-Phytomedicine K. Chitti Kalavathi, Dept. of History, GDC, Nagari. Free radicals and its adverse effects were discovered in the last decade. These are dangerous substances produced in the body along with toxins and wastes which are formed during the normal metabolic process of the body. Free radicals are well documented for playing a dual role in our body as both deleterious and beneficial species. In low/moderate concentrations free radicals are involved in normal physiological functions but excess production of free radicals or decrease in antioxidant level leads to oxidative stress. It is a harmful process that can be mediates damage to cell structures, including lipids, proteins, RNA and DNA which leads to number of diseases. A variety of synthetic medicine employed in the treatment of different diseases also capable to generate free radicals in body which may causes another disease. The plant sources are rich of antioxidants, phyto-constituents are capable to terminate free radical reactions and prevent our body from oxidative damage. Vegetables and fruits are also important sources of antioxidant substances. Different phytoconstituents and herbal product which are safer then synthetic medicine and beneficial in the treatment of diseases caused by free radicals, it also protect the body by prevent the free radicals to cause tissue injury. Phytoconstituents are conferring less side effect and compatible to body physiology. Therefore it is demand of modern era to use such phytoconstituents or phytomedicines. Key words: phytomedicine, Free radicals, antioxidants Preliminary screening of Endophytic fungi from Enicostemma axillare (Lam.) Raynal.for antimicrobial activity Yamilee Das*, Ujwala S. Deepake, G. Gyananath School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded-431606, Maharashtra, India. Enicostemma axillare (Lam.) Raynal.belonging to Gentianaceae family is a perennial herb found throughout India and is reported to possess medicinal properties. Considering the importance of this plant, a study was conducted to determine the colonization frequency of endophytic microbes and to evaluate the antimicrobial activity of crude extracts of fungal endophytes from Enicostemma axillare. endophytic fungi from different parts of the plant (leaves, stem and roots) were isolated, pure cultures were raised and identified based on the morphology and characteristics of fungal spores. The highest colonization frequency of bacteria and actinomycetes was observed in leaves (100 %). However, colonization frequency of endophytic fungi was around 92 % higher in stem. The endophytic fungi that displayed broad spectrum antimicrobial activity include Aspergillus flavus, Penicillium sp., Eurotium sp., Sartorya sp. and Phomopsis sp. Our preliminary results indicated that crude extracts of endophytic fungi of Enicostemma axillare (Lam.) Raynal. may possess some antimicrobial compounds. Keywords: Endophytic fungi, crude extracts, antimicrobial activity, Enicostemma axillare Science Teacher Education: Nurturing Young Minds for Better Health P. Samata & Pavan MNV University College of Education, Telangana University “Sound mind in a sound body in a sound environment” is the well-known saying in the field of health. A healthy body works as a platform where other important qualities can spring. The different dimensions of health of an individual can be viewed under five heads. They are physical, mental, social, emotional and spiritual health. These dimensions of health can be assessed under different areas of health such as knowledge and understanding of International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 19 whole body, nutrition and health, safety health, environmental health, consumer health, social health and responsibility of health. All these are achieved through teaching of Natural Science. The present Teacher Education Curriculum for science teacher does incorporate these aspects of natural sciences, to explore how natural products help in protecting one’s health. Indeed there is a need to evolve a comprehensive science curriculum that can help one to study medicinal values of natural products. In order to save the young lives, there is an urgent need to strengthen science teacher curriculum. This paper attempts to explore into the depths of pedagogy of Biological Sciences especially to understand and recommend the would-be teachers to focus the child’s attention towards those aspects of the vegetation around and their medicinal attributes so that the child grows up with an increasing awareness of the importance of sciences for better health. Keywords: Teacher Education, Health Education, Natural products, Herbal Medicines. Observations on the Vegetation and Flora of Satkosia Tiger Reserve, Odisha K. Chandramohan1*, P.V. Prasanna1& P. Ramachandra Reddy 2 1 Botanical Survey of India, Deccan Regional Centre, Hyderabad, Telangana-500048 2 Department of Botany, Osmania University, Hyderabad *Corresponding author. Email: kolaganicm@gmail.com Documenting flora of any area is vital to unravel the floristic richness and in planning conservation programmes to save the fast eroding Biodiversity. As a part of this scientific programme, the present work on inventorying the flora of the Satkosia Tiger Reserve in Odisha State has been initiated. Satkosia Tiger Reserve ranks second largest home in Odisha for the population of Elephants and Tigers. The reserve comprises two adjoining sanctuaries viz., Satkosia Wildlife Sanctuary and Mahanadi Wildlife Sanctuary. It is a meeting point of two bio-geographical regions of India: Deccan peninsular and Eastern Ghats, contributing to the rich biodiversity. The preliminary floristic survey carried out during 2013-15 in the Tiger Reserve has resulted in identifying 650 species. Satkosia Tiger Reserve display four sub types of forests- Tropical Semi Evergreen forests, Tropical Moist deciduous forests, Tropical Dry deciduous forests and Riparian forests. Tropical Semi evergreen forests are dominant with species like Artocarpus lacucha, Magnolia champaca, Dillenia pentagyna, Mangifera indica, Firmiana colorata & Macaranga peltata and underground vegetation is covered with Curcuma aromatica and Boesenbergia longiflora. In Tropical Moist Deciduous forests, Shorea robusta (Sal) is the dominant plant species with association of Lagerstroemia parviflora, Mallotus philippensis, Syzygium cumini, Bauhinia vahlii, Haldina cordifolia, Pterocarpus marsupium, Madhuca indica, Kydia calycina, Bridelia retusa, Anogeissus latifolia and Garuga pinnata. Tropical Dry Deciduous Forests are covered with Cochlospermum religiosum, Mitragyna parvifolia, Melia dubia, Gardenia latifolia, Tectona grandis, Cleistanthus collinus, Holarrhena pubescens, Wrightia tinctoria, Gardenia resinifera, Ziziphus oenopolia, Terminalia tomentosa and Cassia fistula species. Along Mahanadi River and its tributaries, Dry Tropical Riparian forests characterized by the presence of Terminalia arjuna, Vitex altissima, Strychnos nux-vomica, Anogeissus acuminata, Syzygium cumini and Barringtonia acutangula. Dendrocalamus strictus is dominant bamboo in the Tiger Reserve. Key words: Flora, Vegetation, Satkosia Tiger Reserve, Odisha Effects of Biofertilizers on Yield and Biochemical Parameters of Ashwagandha (Withania somnifera) D. Vijaya Lakshmi, T. Prabhakar Reddy, J. Kamalakar, and C. Padma Veni Krishi Vigyan Kendra, Rudrur, Nizamabad Professor Jayashankar Telangana State Agricultural University, Telangana. A field experiment was conducted to study the effect of organic and inorganic sources of nutrients on physiological and biochemical parameters of ashwagandha. In this study a International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 20 different biofertilizers, chemical fertilizers and organic manure were applied in order to find out their effects on growth and biochemical parameters of ashwagandha. The biofertilizers included Azospirullum, Phosphorous solubilising bacteria (PSB), Azotobacter, rhizobium, combination and control. The test crop was ashwagandha. The results indicated that, among the different organic and inorganic sources of nutrients biofertilizers (Azospirillum, Azotobacter, Rhizobia and PSB) brought about significantly higher chlorophyll contents (‘a’ and ‘b’), carotene content, photosynthetic and respiration rates as well as total alkaloid content over the remaining organic and inorganic sources. However, 100 % recommended dose of fertilisers produced maximum dry matter growth and root yield (12.49 q/ha). Amongst the inorganic sources of nutrients, 100% RDF (N20P40K20) recorded significantly higher of all these physiological and biochemical parameters including root yield (14.02 q/ha) and alkaloid yield (17.41 q/ha) of ashwagandha. Antifungal Activity of Some Important Medicinal Plants against Two Plant Pathogenic Fungi Jalander, V., S. Prashanthi and M. Mamatha Department of Botany, Telangana University, Dichpally, Nizamabad (T.S.) Leaf extracts of five different medicinal plant species such as Adhatoda vasica Nees, Eucalyptus globules Labill.,Ocimum sanctum L., Azadirachta indica A. Juss. andCatharanthus roseus (L.) G. Don.wereextracted with the help of Soxlet apparatusin water and ethanol. These extracts were tested for their antifungal activity against two plant pathogenic fungi i.e. Fusarium oxysporum f.sp.udum Butler, a wilt pathogen of pigeon pea [Cajanus cajan (L.) Millsp.]andAlternaria solani, leaf spot pathogenof tomato (Lycopersicon esculentum Mill.). The results of agar well diffusion assay indicated that the ethanolic leaf extracts were found good inhibitory than aqueous leaf extracts against plant pathogenic fungi tested. The ethanolic leaf extract prepared from Azadirachta indica was shown to have better efficacy against wilt pathogen and extract from E. globules found good inhibitory activityagainst leaf spot pathogen tested. Keywords: Antifungal Activity, Medicinal Plants, Plant Pathogenic Fungi. The Promise of Insulin Plant in Treatment of Diabetes Syed Afzaluddin Biyabani*, Syed Raziuddin Faisal, Javed Akhtar Ansari Department of PharmD, MESCO College of Pharmacy, Hyderabad, TS, India. Chamaecostus cuspidatus, common name Fiery Costus or Spiral Flag, is a species of herbaceous plant in the Costaceae family native to eastern Brazil (States of Bahia and Espirito Santo). In India, it is known as insulin plant due to its use in Ayurvedic medicine for its purported anti-diabetic properties. Chamaecostus cuspidatus has large fleshy looking leaves. The undersides of these large, smooth, dark green leaves have light purple shade. The leaves are spirally arranged around the stem, forming attractive, arching clumps arising from underground rootstocks. In Siddha medicine, it is known as Kostum. The species reproduces vegetative by rhizome and birds disperse seeds when they feed on the fruits. Consumption of the leaves are believed to lower blood glucose levels, and diabetics who consumed the leaves of this plant did report a fall in their blood glucose levels. Regular consumption of insulin plant leaves in conjunction with other modalities of treatment has effectively provided glycemic control in diabetics; the dose of insulin could be reduced to half. Blood sugar levels which were earlier not controlled with oral hypoglycemic drugs or non-allopathic treatments can be controlled with this plant. The risk of diabetic complications is also been avoided as no adverse effects due to the consumption of insulin plant leaves were reported in studies. Regular consumption is necessary for benefits to be observed. Besides, it has been proven to possess various pharmacological activities like hypolipidemic, diuretic, antioxidant, antimicrobial, anti-cancerous etc. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 21 Heavy metals in Herbal preparations P. Manohar Dept. of Zoology, SVA Govt. Degree College, Srikalahasthi. The object of Ayurveda is to cure the disease and to prevent the onset of disease. To achieve this aim various diet and drug materials were mentioned in ayurvedic literatures. Due to easy availability, the use of herbal material and its preparation were frequently used for nutritional and medicinal purpose. Globally, herbal medicine is three to four times more commonly practiced than conventional medicine. The WHO estimates above 60% of the world's population use traditional medicine as their primary form of health care. Some traditional medicine/herbal preparation with ancient formulas have been found to contain some heavy metals, such lead, cadmium, mercury, arsenic and lithium etc. Even though the herbal preparations are safe, but some of herbal preparations causes serous poisonings and toxic effect due to the preparation containing dangerous toxic drugs or heavy metals. Both medical professional and the general public should be alerted to the potential toxicity of herbal preparation. There should be frequent monitoring of herbal preparations, containing toxic drugs or heavy metals. For the purpose of monitoring the herbal preparation contamination with heavy metal like Lead, a strength quality control of herbal raw materials/its preparation needed to check the presence of heavy metals by using modern sophisticated instruments like Atomic absorption spectrophotometer, flame photometer etc. The limits (parts per million) of such heavy metals in herbal preparation should remain within specification. Keywords: Metals, Non-metals, Lead poisoning Pollen analysis of honeys from Hukumpeta mandal of Paderu forest division in Visakhapatnam district R. Devender and H. Ramakrishna* Department of Botany, University College of Science, Osmania University, Hyderabad *Corresponding author Email: hrkpaleobot@gmail.com Pollen analysis of nine Apis dorsata, A.florea, A.cerena and Trigona Sp. honey samples from various localities of Hukumpeta mandal of Paderu forest division in Visakhapatnam district, Andhra Pradesh State were studied and recorded Schleichera oleosa (58%), Eucalyptus globulus (73%) and Brassica nigra (57%) as Predominant pollen types in 3 honey samples and identified as unifloral. In these honeys samples besides the predominant pollen types Tridax procumbens, Ageratum conyzoides, Syzygium cumini, Exacum bicolour, Mimosa pudica, Pulicaria foliosa, Erythrina indica, Hakea laurina, Vernonia cinerea and Borassus flabellifer were constituted as other significant pollen types. Diversity of honeybees and diversity of bee forage plant for these bees were recorded from Hukumpeta mandal of Paderu forest division indicates to establish apiary. Hence useful to increase honey production in large scale which will be useful to increase the economic status of the local people by marketing the honey and also useful for the production of more quantity of medicinally and qualitatively pure honey. This study is also useful by knowing beeforage plants of different types of Apis sps. which were already established both in Telangana and Andhra Pradesh states, for more production of honey through apiary. Key words: Pollen analysis; Unifloral honey; Human welfare. Role of Coarse grains & Millets in Human Health– A Review A. Poshadri, G. Praveen Kumar* and J.Sai Prasad College of Food Science & Technology *Regional Sugarcane and Rice Research Station, Rudrur, Nizamabad. Professor Jayashankar Telangana State Agricultural University, Hyderabad, Telangana. Coarse Cereals comprises crops like jowar, bajra, ragi, other small millets (kudo, kutiki, sanwa, foxtail) and maize, which have traditionally been the main components of the International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 22 food basket of the poor in India. As per available estimates, total coarse grains production in Telangana State is 4.0 million tones during 2012-13 with a share of 12 % of total country’s output (State of Indian Agriculture 2012-13, Government of India). Coarse cereal grain based products with health benefits represent a growing part of the health related food market. The adoption of health claims linking whole grain based products with a reduction of risk of coronary heart disease significantly supported the major market growth in recent years. In addition, coarse grain &millet products are associated with a range of other health benefits, including a reduced risk of type-2 diabetes, obesity, and some types of cancer. In fact, the recent coarse grain & millet trend is a reversal of the main trend in the past century – towards highly refined products, without the outer part of grains. These outer parts are rich in fiber and a wide range of phytochemicals. Whereas market growth of coarse grain & millet products is expected to continue in the coming years, research in India and other parts of the world is focusing on exploring options for a wider range of healthy cereal grain based products, based on a deeper understanding of the mechanisms of coarse grain & millet health benefits. Finance Commitees for AYUSH G. Padmavathy Customer Support Officer, State Bank of India, Miryalaguda, Telangana India has rich diversity of medicinal plants. Medicinal Plants form the major resource base of our indigenous health care traditions. The outreach and acceptability of AYUSH systems, both nationally as well as globally, are dependent on uninterrupted availability of quality medicinal plants based raw material. More than 90% of the species used in trade continue to be sourced from the wild of which about 2/3rd are harvested by destructive means.The chairman of the SFC will have authority to invite, as special invitees, representatives from organisations engaged in R&D, quality control, planning and other related disciplines in medicinal plants sector. The Board has a Standing Finance Committee(SFC), which will be headed by Secretary (AYUSH). This committee will act as the Executive Committee of the Mission. The National Medicinal Plants Board (NMPB) will provide the necessary support to both SFC and the Mission. The Chief Executive Officer, NMPB who is of the rank of Joint Secretary will function as the Mission Director. The CEO has been delegated the powers of the Head of the Department and would, therefore, be able to carry out the Mission mandate and also implement and monitor the action plans/proposals approved by the SFC. The Standing Finance Committee(SFC) will have the following functions: (i) To oversee the activities of the Board and approve its annual budget. (ii) To consider and approve any financial proposal which is to be included for expenditure in the Budget. (iii) To consider and approve any proposal for incurring expenditure. (iv) To consider and recommend all proposals for creation of new posts. (v) To consider and allocate resources among the states and under different components of the scheme. (vi) To consider and approve projects under existing pattern of assistance. (vii) To consider and approve components of individual projects for which cost/subsidy norms have not been prescribed. (viii) The SFC shall also monitor the overall subsidy being disbursed under the scheme and in particular monitor the cap of 30% for cultivation subsidy. (ix) Any other matters that the Board may refer to it. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 23 Antifungal Effect of Some Weed Plant Leaf Extracts on Seed-borne Fungi of Pigeonpea [Cajanus cajan(L.) Millsp.].Seeds Jalander, V. and B.D. Gachande* Department of Botany, Telangana University, Dichpally, Nizamabad (T.S.) *Botany Research Laboratory and Plant Disease Clinic, N.E.S. Science College, Nanded. Seeds are regarded as means of transporting plant pathogens. Seed borne diseases affect growth and productivity of crop plants. A seed borne pathogen may cause seed abortion, seed rot, seed necrosis, reduction or elimination of germination capacity, as well as seedling damage. Different types of chemicals have been used to control these pathogens. Use of plant extracts against plant pathogenic fungi is however, a recent approach to plant disease control. It helps to avoid environmental pollution by chemicals. In the present study ten commonly growing different weed plants leaf extracts i.e. Acalypha indica L., Achyranthes aspera L., Alternanthera sessilis (L.) R. Br. Ex. DC., Cassia tora L., Datura stramonium L., Euphorbia hirta L., E. heterophylla L., Parthenium hysterophorous L., Solanum xanthocarpum Schrad. & H. Wendl.andXanthium strumarium L.,were tested for their antifungal activity (in-vitro) against seed-borne fungi of pigeonpea [Cajanus cajan (L.) Millsp.]. All the alcoholic plant leaf extracts showed good inhibitory activity against seedborne fungiand increased percent of seed germination, root & shoot length and vigour index. Alcoholic extracts of X. strumarium and D. stramonium were showed very good antifungal activity than the other plants tested. Next to these, the alcoholic extracts from E. hirta, and C. tora showed good inhibitory activity against the seed-borne fungi of pigeonpea. Water extracts of test plants also showed antifungal activity (less than the alcoholic extracts) against seed-borne fungi of pigeonpea. Seeds of pigeonpea treated with the alcoholic extract of X. strumarium gave 98.00% seed germination. The highest vigour index was recorded after the application with alcoholic and water extracts of X. strumarium and D. stramonium on seeds of pigeonpea while the lowest was observed in case of untreated control. Key Words: Antifungal activity, weed plants, seed-borne fungi, pigeonpea. Organic Farming of Medicinal and Aromatic Plants: A Hope for Quality Enhancement T. Prabhakar Reddy, D. Vijaya Lakshmi, J. Kamalakar and Dr. B. Joseph Regional Sugarcane and Rice Research Station, Rudrur, Nizamabad Professor Jayashankar Telangana State Agricultural University, Hyderabad, Telangana. India has traditionally been a country of organic agriculture, but the growth of modern scientific, input intensive agriculture has pushed it to wall. But with the increasing awareness about the safety and quality of foods, long term sustainability of the system and accumulating evidences of being equally productive, the organic farming has emerged as an alternative system of farming which not only address the quality and sustainability concerns, but also ensures a debt free, profitable livelihood option. Medicinal plants in every sense play an important role in day to day activity of people and would serve tremendously if grown organically for better health management. Pot experiments conducted in the laboratory have demonstrated that adding vermicompost has resulted in increased organic carbon content of the soil with larger pools of stored nutrients that are critical for long term soil fertility. The seedlings of Tagetes minuta, Ocimum basilicum, Hyoscyamus muticus and Silybum marianum grown in vermicompost supplemented pots were healthier with higher root and shoot weights compared to seedlings grown in FYM supplemented pots. Application of organic manures reduces the incidence of various seedling diseases such as those caused by Pythium sp. and Rhizoctonia sp. in opium poppy (Papaver somniferum), ashwagandha (Withania somnifera) and aambrette (Abelmoschus moschatus). Growth characters, yield, essential oil and its constituents, fixed oil, carbohydrates, soluble sugars and nutrients contents of medicinal and aromatic plants were significantly affected by adding the biological fertilizers compared with recommended International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 24 chemical fertilizers. The demand for organic essential oil generated from aromatherapists and flavor industries, are keen to use only high quality organic essential oils, also called clinical, pure or therapeutic oils. Such oils are usually priced 100% higher than normal oils. Our experience also reveals, organically farmed soils had better tilth. The changes in structure brought about by sufficient organic matter improved ground cover, while reduced water runoff by 10-50%, increased filtration by 10-25% therefore such soils are less prone to drought with a significant reduction in irrigation costs. There is also evidence to show nutrient cycling/fixing microbes are in plenty, and are efficient in organic soils making more nutrients available to the soil. On macroeconomics levels, conversion to organic farming would yield higher benefits with greater eco-compatible implications. It would reduce depletion of fossil fuel, reduce social costs associated with erosion, improve wild life/livestock habitats and ensure the productivity of land for future generations. In addition, it would facilitate foreign trade and help build valuable foreign exchange, and where trade is constrained because of foreign currency, organic agriculture becomes a solution to economic necessity. Pharmacological Profile of Indian Solid Gold (Curcumin) Syed Raziuddin Faisal*, Syed Afzaluddin Biyabani, Javed Akhtar Ansari Department of PharmD, MESCO College of Pharmacy, Hyderabad, TS, India. Turmeric, derived from the plant Curcuma longa, is a gold-colored spice commonly used in the Indian subcontinent, not only for health care but also for the preservation of food and as a yellow dye for textiles. Curcumin, which gives the yellow color to turmeric, was first isolated almost two centuries ago, and its structure as diferuloylmethane was determined in 1910. Since the time of Unani & Ayurveda (1900 BC) numerous therapeutic activities have been assigned to turmeric for a wide variety of diseases and conditions, including those of the skin, pulmonary, and gastrointestinal systems, aches, pains, wounds, sprains, and liver disorders. Extensive research within the last half century has proven that most of these activities, once associated with turmeric, are due to curcumin. Curcumin has been shown to exhibit antioxidant, anti-inflammatory, antiviral, antibacterial, antifungal, and anticancer activities and thus has a potential against various malignant diseases, diabetes, allergies, arthritis, Alzheimer's disease, and other chronic illnesses. These effects are mediated through the regulation of various transcription factors, growth factors, inflammatory cytokines, protein kinases, and other enzymes. Curcumin exhibits activities similar to recently discovered tumor necrosis factor blockers, vascular endothelial cell growth factor blocker, human epidermal growth factor receptor blockers and a HER2 blocker. Considering the recent scientific bandwagon that multitargeted therapy is better than monotargeted therapy for most diseases, curcumin can be considered an ideal "Spice for Life". Remediation of heavy metals effect on stomata cells in Curcuma longa L. Damera Vineeth1*, Chinna. Venkateshwar 2 and Syeda Azeem Unnisa3* Department of Botany, University College of Science, Osmania University, Hyderabad Email:1.vineethdamera@yahoo.com, 2. cvlaxman2004@yahoo.com, 3. syeda_30@yahoo.co.in (corresponding author) The present research study was conducted to know the heavy metal ( Cd,Cr and Ni) toxicity in stomatal cells Curcuma longa L. in leafand application of Ca(OH)2 for its remediation. Pot culture experiments were conducted with three different treatments till productivity levels at Greenhouse of Botanical Garden, Department of Botany, Osmania University, Hyderabad. The three treatments consist of control without any addition of heavy metals to the soil, Treatment -I heavy metals spiked into the soil and Treatment-II, 1 % of calcium hydroxide added along with heavy metals to the soil. The results showed when compared to control and Treatment-II the high concentrations of heavy metals (Ni, Cd and Cr) are found in leaf of Curcuma longa L. in (Treatment- I). Leaf epidermal studies mainly stomatal cells, costal International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 25 cells, leaf clearings and articular preparations were examined with light microscope. Anisocytic stomata observed in Control,Treatment- I and Treatment- II. It was also observe that the the no.of stomatal cells are increased in Control and Treatment -II when compared to Treatment –I and the heavy accumulation in the leaf parts.. In addition, the plants grown in treatment -II with 1% calcium hydroxide treated soil, reversed the growth suppression and inhibited the heavy metal toxicity in plants as evidenced by reduced heavy metal concentration in leaf. Anatomical properties of plant parts are sources for taxonomic inferences in different groups of medicinal plants. The aim of present study is to use stomatal cells number and characters as aid in taxonomy of medicinal plants crops. The study would help in the identification and authentication of these medicinally important plants on the basis of stomatogenesis.The study concludes that medicinally important plant Curcuma longa L.affected with heavy metals can be treated by using calcium hydroxide. Keywords: Curcuma longa L., Heavy metals influence, Treatment.1%Ca(OH)2.Stomatogenesis. Origin of Herbal medicine: A critical review G. Tirumala Vasu Deva Rao, Dept. of History, Government Degree College, Nagari The pharmacological treatment of disease began long ago with the use of herbs. By definition, ‘traditional’ use of herbal medicines implies substantial historical use, and this is certainly true for many products that are available as ‘traditional herbal medicines’. The ancient Chinese, Indians, Egyptians, Babylonians, and Native Americans were all herbalists. The oldest known list of medicinal herbs is Shen Nung’s Pen Ts’ao or Shennong Ben Cao Jing (c. 3000 B.C.), a Chinese herbal that is probably a compilation of an even older oral tradition. The ancient Greeks and Romans were also renowned herbalists. Surgeons travelling with the Roman army spread their herbal expertise throughout the Roman empire, in Spain, Germany, France, and England. Dioscorides (c. 40-c. 90) and Galen (131-200 A.D.), both Greek surgeons in the Roman army, compiled herbals that remained the definitive materia medica texts for 1500 years. Through the Middle Ages, herbalism was preserved in the monasteries of Britain and mainland Europe. Before the establishment of universities in the eleventh and twelfth centuries, monasteries served as medical schools. Monks copied and translated many of the works of Hippocrates, Dioscorides, and Galen. Their “physick” gardens, well-stocked with the most common and useful medicinal herbs, served as basic training grounds for the next generation of physicians–monks and laymen alike. Meanwhile, as a result of the Islamic conquest of North Africa in the seventh and eighth centuries, Arabic scholars acquired many Greek and Roman medical texts. Iranian physician Ibn Sina, also known as Avicenna (980-1037 A.D.), combined the herbal traditions of Dioscorides and Galen with the ancient practices of his own people in The Canon of Medicine (al-Qanun fi at-tibb). One of the most influential medical texts ever written, Avicenna’s Canon spread through Europe during the eleventh and twelfth centuries. With the invention of the printing press in the mid-fifteenth century, the herbals of Dioscorides, Galen, and Avicenna were mass-produced and made accessible to people outside the palace, the monastery, and the university. Use of the herbals required no specialized skills: readers simply gathered the herbs and applied them in the prescribed manner and dosage. Key words: Herbal medicine, History Antimicrobial Activity of Some Important Medicinal Plants Jalander, V., A.A. Haleem Khan and B. Vidya Vardhini Department of Botany, Telangana University, Dichpally, Nizamabad (T.S.) Email: jalandervaghmare@gmail.com Leaf extracts of four different medicinal plant species [Adhatoda vasica Nees, Eucalyptus globules Labill.,Ocimum sanctum L. and Catharanthus roseus (L.) G. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 26 Don.]wereextracted with the help of Soxlet apparatusin water, hexane and ethanol. These extracts were examined for their antimicrobial activity against three pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Salmonella typhi.The results of agar well diffusion assay indicated that the ethanolic leaf extracts were found good inhibitory than aqueous and hexane leaf extracts against pathogenic bacteria tested. The ethanolic leaf extract prepared from Eucalyptus globules Labill was shown to have better efficacy against Escherichia coli and Staphylococcus. Ethanolic leaf extract from Ocimum sanctum L. found good inhibitory activityagainst Salmonella typhi. Keywords: Antimicrobial Activity, Medicinal Plants, Pathogenic Bacteria. Phytonutrient and Antioxidant Enzyme Studies in Phoenix Fresh Ripened Fruit B.Thirupathi* and S. Gangadhar Rao. Department of Botany, Osmania University, Hyderabad Email: thirupathi.bunga@gmail.com, gangadharrao53@gmail.com Phytonutrients and antioxidant enzymes studies were undertaken in phoenix fresh ripened fruit. Among the observation phenols, flavonoids and anthocyanins contents was recorded maximum, however ascorbic acid, total carotenoids and beta carotene was present in low quantities. In case of antioxidant enzymes catalase activity was high with (3.233 units/g) as compared to peroxidase and polyphenol oxidase (0.012 and 0.050 units/g) respectively. Super oxide dismutase enzyme activity was recorded high with 20 units/g, however glutathionreductase activity was low(0.353 units/g). The result of antioxidant assay showed maximum inhibition % in FRAP (1.64 %). There was very little inhibition% activity with ABTS, DPPH. Key words: phytonutrient, Antioxidant enzyme, SOD, glutathionreductase. Health Benefits of Green Tea A. Srilakshmi, Department of Computer Application, GDC (W),Srikalahasthi. Green tea in its purest and unaltered form has always influenced health from several generations. Many researchers have revealed green tea poly-phenol and catechins as powerful anti-oxidant potential helps in prevention of cellular damage from oxidative reactions in the body. Several epidemiological studies have also shown beneficial effects of green tea in cancer, cardiovascular, and neurological diseases. Several vitro and in vivo studies have been carried on cancer chemoprevention, hypercholesterolemia, artherosclerosis, Parkinson's disease, Alzheimer's disease, and other agingrelated disorders. Various phytochemicals present in the green tea are useful for the treatment of various ailments. Our present study is to elucidate the various health benefits of different phytochemicals present in green tea. Key words: Green tea, Antioxidants, Phytochemicals In vitro production of Spilanthol – A medicinally important secondary metabolite from hairy root cultures of Spilanthes acmella Hajera Sana* and A.Sabitha Rani Dept. of Botany, Osmania University College for Women, Koti, Hyderabad Email: hajera.sana@gmail.com Abstract Spilanthes acmella Murr. (Family Asteraceae) is an important medicinal plant with antimicrobial, antioxidant and insecticidal activity. The active chemical component is spilanthol, an alkamide which is present in roots and all aerial parts of the plant. Spilanthol is being used in pharmaceuticals, toothpaste and cosmetic industry. Since there is less commercial cultivation of S.acmella,spilanthol production is not meeting the industrial demand.Hence there is a need to produce spilanthol by alternative methods. Hairy roots transformed with Agrobacterium rhizogenes have been found to be suitable for large scale International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 27 secondary metabolite production because of their genetic and biochemical stability and high productivity in hormone free culture conditions. Hairy roots were induced from the S.acmella leaf explants by transforming with Agrobacterium rhizogenes (MTCC 532). Initiation of hairy roots occurred after 10 days of infection with a high transformation frequency(70 %).The confirmation of transformation was done by Polymerase Chain reaction (PCR) by amplification of rol B and rol C primers responsible for T-DNA transfer. The hairy roots were mass multiplied on hormone free ½ MS liquid medium and subcultured in fresh medium every 15 days.The active component spilanthol was extracted from dried hairy roots using cold extraction method and its HPLC analysis showed substantial amount of spilanthol in the hairy roots of Spilanthes acmella. This study is useful for scaling up of hairy roots of S.acmella in bioreactors for large scale production of spilanthol and can be used as a model system in metabolic engineering. Phytochemical Investigation of Curcuma Longa M. Thejomoorthy Dept. of Zoology, GDC, Rajahmundry In recent year there has been rapid increase in the standardization of selected medicinal plant of potential therapeutic significance. The rhizome of Curcuma longa reported to have good medicinal values in traditional system of medicines. The present study deals with pharmacognostic parameters for the rhizomes of Curcuma longa which mainly consist of Macromorphology, Physico-chemical constants and Phytochemical screening. This information will be used for further pharmacological and instrumental evaluation of the species and will assist in standardization for quality, purity and sample identification. The alcohol soluble extractives were also determined. Preliminary phytochemical analysis of ethanol extract was carried out. The results were positive for steroids terpenoids, phenols, tannins and flavonoids. HPTLC analysis confirms the presence of Curcuminoids. These secondary metabolites were the active constituents of Curcuma longa Linn. that may be responsible for its pharmacological activities. Keywords: Curcuma longa, Curcuminoids, Rhizome, Physicochemical constant Medicinal Properties of Sapindaceae Family Devaraju Srinivas, K. Jaganmohan Reddy* Department of Botany, Telangana University, Dichpally, Nizamabad, (T. S.) India *Department of Botany, Kakatiya University, Warangal, (T. S.) India Email: devarajusrinu@gmail.com The Sapindaceae or Soap berry family contains more than 2,000 species from 150 genera, mostly trees and shrubs, but rarely herbs, widely distributed throughout the warm sub-tropics and tropics. The majority of species are native Asia. Although there are few species reported from South America, Africa and Australia. New species are still being described. The most specialized growth forms are the rather strange unbranched palm-like trees such as Talisia and woody climbers like Sejania and Paullinia. The largest trees including Schleschesa oleasa and Pometicapinnata may reach up to 60m in height. Sapindus is a genus of about five to twelve species of shrubs and small trees in the Lychee family, Sapindacecea native to warm temperate to tropical regions in both the old world and New world. The genus includes both deciduous and evergreen species. Members of the genus are commonly known as soapberries or soapnuts because the fruit pulp is used to make Soap. The generic name is derived from the Latin words saponis meaning "soap" and indicus meaning "of India”. (Soap berry), a genus of plants of the family Sapindaceae. They are evergreen or deciduous trees. The pinnate leaves are alternate, and the flowers are in terminal or axillary panicles. There are about 15 species, distributed in the tropics of Asia and America. The fruits, which contain up to 38 percent saponins, are used as soap. Soapberries are cultivated in tropical and warm countries as ornamentals and for their saponins. Three species-Sapindus International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 28 saponaria, S. mucorossii and the winter-hardy American species S. drummondii-are grown in the USSR, on the Black Sea shore of the Caucasus and, less frequently, in Transcaucasia and Middle Asia. The genus’s Russian common name, myl’noe derevo, is sometimes used to designate the goldenrain tree (Koelreuteria paniculata) of the same family, which grows in East Asia and contains saponins in its bark. Keywords:Medicinal properties, Sapindaceae, Soap nuts. In vitro antagonistic activity of Trichoderma harzianum and Trichoderma viride against Alternaria alternata, the leaf spot pathogen of Senna plant Nagamani Tagaram and Bhumi Narasimha Reddy Department of Botany, Osmania University College for Women, Koti, Hyderabad. Email: nagamanitagaram@gmail.com Medicinal plants are valued for the content and chemical composition of their bio-active components. The intensive use of fungicides for the control of diseases in medicinal plants has resulted in the accumulation of toxic chemical, seriously affecting their marketing and utilization in the development of plant based formulations. The increasing awareness of fungicide related hazards has emphasized the need for adopting a safer biological method. The genus, Trichoderma is ubiquitous with high population density has been extensively studied for their biological control of fungal diseases of plants. Hence, the present study was taken up for bio-control of foliar disease of Senna (Cassia angustifolia Vahl), an important medicinal plant with excellent export market potential. The antagonistic activity of Trichoderma viride and Trichoderma harzianum isolates were tested in vitro against the Alternaria alternata, the causal organism of leaf spot disease on Senna. The dual culture plate method has revealed that the species of Trichoderma has excellent biocotrol properties against the leaf spot pathogen. The percentage growth inhibition of Alternaria alternata byTrichoderma viride and Trichoderma harzianum was 80.1% and 72.2% respectively. The results indicated that the growth inhibition of test pathogen by Trichoderma viride and Trichoderma harzianum provides an excellent potential antagonist capable of controlling the pathogenecity of Alternaria alternata. Medicinal Value of Rice Land Races in India N.Sandhyakishore*, G.Praveenkumar, M.Pallavi, T.Prabhakar Reddy, J.Kamalakar, Firdoz Shahana, Y.Bharathi and B.Joseph *Corresponding Author: N.Sandhyakishore, Scientist (Plant Breeding), Regional sugarcane and Rice Research Station, Rudrur, Nizamabad-503188. Email: kishoregene@gmail.com Rice is the staple food of over half of the world’s population and 90% of Asians. Ancient records speak of the existence of rice varieties of curative value for various ailments, as detailed in Ayurvedic treatise of the 15th and 16th century AD. India is endowed with very rich rice diversity of speciality rices with unique quality features. The rice varieties identified in Chhattisgarh viz., ‘Aalcha’ for the treatment of pimples, Maharaji, which gives strength and stamina to ladies immediately after delivery, ‘Baisoor’ for epilepsy, ‘Laicha’ for pregnant women to deliver healthy children are a few examples and the ‘Baisoor’ variety is used for the treatment of headache and boils in different regions. It is also used for skin diseases, ringworm infection, itching and conjunctivitis, and as a face pack with neem leaves. Another variety, ‘Njavara’, is a unique land race with great antiquity and traditionally used for the treatment of rheumatism, arthritis, neurological problems and for the relaxation as well as rejuvenation of weak muscles of aged persons by the Ayurvedic physicians of Kerala. ‘Njavara’ is used as a supplementary diet for underweight persons or consumed as a replenishing drink called ‘karkada kanjhi’ during monsoon season along with some herbal medicines. The oil prepared from this variety is used for a wide range of ailments like aches, painful inflammations, spondylitis, lower backpain, etc. But the most significant use is the International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 29 medicinal pouch named ‘njavarakizi’, made with this rice after cooking it in a milk decoction of medicinal herb, Sida cordifolia. It is applied as a massage on the whole body, dipping the pouch repeatedly in the decoction. The medicinal and nutritive properties of ‘Njavara’ have recently received wide recognition and drawn the attention of some corporate sectors for its commercial exploitation. Some other medicinal rice varieties still available in Kerala are ‘Jaathi Suggi’, ‘Jeeraka Chembavu’, ‘Karutha Chembavu’, ‘Kamaal’, ‘Kolaran’, ‘Naron’, ‘Nalla Chennellu’, ‘Vadakkan’ and ‘Vatten’. In Odisha, the tribal inhabitants consume rice beer colloquially called ‘Handia’ prepared from traditional rice varieties by fermenting watered rice for several days with a herbal formulation called ‘Bakhara’ into it and prepare health drink claimed to prevent gastro-intestinal ailments. Similar practice is observed among the tribals of North Bengal, who consume an alcoholic beverage called ‘Haria’. But in modern agriculture, farmers are gladly accepting the new varieties for higher yield. Thus millions of years of genetic diversity and germplasm heritage are vanishing forever. Hence urgent attention is warranted to collect and conserve these unique rice varieties and also focus on the improvement, production, processing and marketing of such rice varieties as we have been doing for basmati rice for the benefit of prosperity. Effect of Aqueous Extracts of Datura sp. Against Fusarium oxysporum f.sp.udum, Wilt Pathogen of Pigeonpea [Cajanus cajan (L.) Millsp.]. Jalander, V. and B.D. Gachande* Department of Botany, Telangana University, Dichpally, Nizamabad (T.S.) *Botany Research Laboratory and Plant Disease Clinic, N.E.S. Science College, Nanded. Green plants represent a reservoir of effective chemotherapeutants and can provide valuable sources of natural pesticides. Secondary plant metabolites and plant based pesticides appear to be one of the better alternatives as they are known to have minimal environmental impact and danger to consumers in contrast to synthetic pesticides. Extracts of many higher plants have been reported to exhibit antifungal, antibacterial and antiviral properties under laboratory and field trials. Allthe Datura species contain tropane alkaloid such as hyoscyamine, scopolamine and atropine in their parts such as root, stem, leaf, flowers and seeds. Besides its hallucinogen activity, Datura reported to have antimicrobial activity. The effect of aqueous root, stem and leaf extracts from four different species of Datura viz. D.stramonium L., D. innoxia Mill. Gard, D. metal L.and D. ferox L. Amoen.were tested for their antifungal activity against Fusarium oxysporum f.sp. uduma wilt pathogen of pigeonpea [Cajanus cajan (L.) Millsp.].The extracts of Datura sp. at 5, 10, 15 and 20% concentrations were incorporated in glucose nitrate (GN) medium. The extracts of Datura sp.at 20% concentration was found more inhibitory activity againstthe pathogen tested. The leaf extracts were more effective in reducing the growth of wilt pathogen than the other plant parts used. Key words:Datura sp., antifungal activity, Fusarium oxysporum f.sp. udum Azadirachta indica The Medicinal Plant in Poultry Birds Syeda Zainab, Research scholar, Maulana Azaad College, Dr BAMU, Aurangabad.M.S. Azadirachta indica also known as neem, neem tree belongs to the family Meliacea. Since ancient time it was using in different fields like ayurveda, unani and homeopathic system of medicine for treatment of health problems as it is having anti-bacterial, anti inflammatory, anti oxidant, anti parasitic, anti helmintic activities. It is most important plant whose almost every part like leaves, flower, seed, fruit, root and bark is used for the medicinal purpose. From neem tree near about 140 compounds isolated and is prepared from above mentioned parts of the plant. Parasitism is a relationship between two different organisms in which smaller is called as parasite living on the body of another organism called as host who provides the platform for International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 30 the parasite to live due to which there are side effects on the health of host in the form of diseases that means hosts are sufferers due to the parasite presence. In this the best example for the parasitism is the cestode parasites which are living in the intestine of poultry birds and are responsible for the less egg laying capacity, loss of apetite, less flesh production. But by the using of neem plant we can cure the poultry from parasite infection. And the outcome is we are getting high egg laying, healthy poultry birds so due to which we can improve the economic value. Phytochemical screening and in vitro anti-oxidant activity of Bacopa monnieri M. Sharmila Department of Zoology,GDC (W), Srikalahasthi. Bacopa monnieri is a medicinal plant, which has been traditionally used in most of the neurological diseases. Qualitative estimation of methanolic and aqueous extract indicates the presence of alkaloids, flavanoids, phenols, steroids, saponins and essential oils. Estimation of total phenols, flavanoids, alkaloids and saponins has been carried out to determine quantitavely. In vitro antioxidant activity such as H 2O2 scavenging activity, thiobarbituric acid (TBA) scavenging activity and nitric oxide scavenging activity showed a potent antioxidant and radical scavenging activity in methanol extract. The Phytochemical and antioxidant activity results were taken into consideration to carry out the further investigation. Keywords: Bacopa monnieri, Phytochemical screening, in vitro antioxidant assay Phytochemical screening, antibacterial and antioxidant properties of Thevetia peruviana(Pers.) K. Schum S. Thejaswi1* andM. Uma Devi2. 1 Department of Biochemistry, St Mary’s College, Hyderabad, Telangana. 2 Department of Biotechnology, Aurora College, Hyderabad, Telangana. Email id: thejaswi@stmaryscollege.in Thevetia peruviana (Pers.) K. Schum,belongs to family Apocynaceae and commonly called as Mexican oleander, Yellow oleander or lucky nut tree. It is an ornamental plant and mostly all the parts of plant (flowers, leaves, seed and root) are medicinally used. In the present investigation the presence of phytochemical constituents, antibacterial activity and antioxidant properties of the root, leaf and stem extracts of the plant are studied. Methonolic and aqueous extracts of different plant parts were obtained and assessed for their antioxidant as well as antibacterial activity. These activities were determined by using standard protocols with some modifications. The antibacterial activity was observed against test organisms like Escherchia coli, Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa. The results show that the inhibitory action of the methonolic extract was relatively more than that of aqueous extract. The phytochemical evaluation reveals the presence of Alkaloids, Flavonoids, Saponins, Tannins, Terpenoids etc. This investigation finds that methanolic extracts of Thevetia peruviana has significant antioxidant activity. The plant extracts show promising free radical scavenging activity and comparable activity against gram positive and gram negative bacteria. There is a good scope to develop natural drugs to reduce oxidative stress and to fight against bacterial pathogens. Key words: Thevetia peruviana, phytochemical constituents, antibacterial activity, antioxidant properties. Medicinal flora described in Lalitāsahasranāmastothram and their significance Devarinti Srihari Reddy, Govt. Degree College, Ramannapet, Nalgonda Dist. 508113. Lalitāsahasranāmastothram is one among the most popular stothras enchanted in the Hindu rituals. It is taken from Uttarakanda of Bramhandapurana. Lord Hayagriva gave this stothra to great sage Agasthya. Goddess Sri Lalitha Devi is depicted by thousand names with the descriptions about flowers and creepers of Michelia champacaL.(Champaca), Saraca International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 31 asoca(Roxb.)Wilde.(Asoka), Calophyllum inophyllum L. (Punnaga), Punica granatum L.(Dadimi), Anthocephalus cadamba (Roxb.)Miq.(Kadamba), Nelumbo nucifera Gaertn.(Kamala/Padma), Stereospermum chelonoides (L.f.) DC.(Patali), Bauhinia purpurea L.(Mandara), Santalum album L.(Chandana)as her ornamentations. All these plants have medicinal properties according to the recent research findings and cure many diseases. In vitro Propagation of Spine Gourd (Momordica dioica. Roxb.) V. Prathap Reddy, A. Ravi Charan, Ranjit Kumar Nagireddy PRR Biotech Innovations Pvt. Ltd. Spine gourd (Momordica dioica Roxb.) commonly known as kakrol / spine gourd, a cucurbitaceous crop (2n=28) originated in the Indo-Malayan region (Rashid, 1976; Singh, 1980, Karim and Ullah, 2011). It is an important rainy season vegetable which is commercially grown in certain districts of Telangana and Andhra Pradesh with high food value, containing good amount of carotene (162 mg/100 g) and protein (3.1 g/100 g) amongst all Cucurbitaceous vegetables (Gopalan et al., 1982). Traditionally the fruits, leaves and tuberous roots are used by the diabetic patient. The plant was also reported to possess anti allergic, antimalarial, anti-feedant, nematocidal and antibacterial properties (Nabi et al., 2002), anti-oxidants, hepatoprotective (Jain et al., 2008) and used as a remedy of jaundice and bleeding pile (Deokule, 2008). The leaves are used as an aphrodisiac, to remove intestinal parasites, treatment of fever, asthma and piles. The roots (paste) of female plant were used to heal bleeding piles and also used for the treatment of kidney stones, jaundice, ulcers. The fruit is considered pungent, bitter, hot, alexiteric, stomachie and luxative and plays a role in cures for biliousness, asthma, leprosy, bronchitis, fever, tumors, urinary discharges, excessive salivation, and heart disease, Juice of the fruit is a domestic remedy for inflammation (Kumar and Prajapati, 2003). Spine gourd is effective against acute renal failure (Jain and Singhai, 2009) and Ethanol and aqueous extracts of spine gourd had antioxidant and hepato-protective activity (Jain et al., 2008). In spite of its multiple uses, the crop remained underutilized and underexploited. As a crop, kakrol has a number of problems, including low yield, Fruits become inedible at maturity owing to the presence of large number of hard seeds, low rate of tuber production (10-20 tuberous pieces per year); germination of seeds is very low or impossible due to hard seed coat (Rashid 1976, Mondal et al., 2006), non-availability of improved varieties, difficulties in propagation by seed due to dormancy, dormancy of tubers and unpredictable sex ratio in seedling progeny (Ali et al., 1991, Mustafa et al., 2013 ). Presently its propagation entirely depends partly on underground tuberous and largely on seed. Maintaining tuber quality in field conditions as well as to conserve it in storage is difficult. Maintaining optimum female to male ratio 10:1 through seed propagation is also difficult. Micro-propagation may help in overcoming these problems to a great extent. An attempt of in vitro propagation of this plant was demonstrated by many workers (Hoque et al., 1995; Nabi et al., 2002; Karim and Ullah, 2011, Devendra et al., 2009; Rai et al., 2012; Mustafa et al., 2013). They have not been many successful stories of commercialization on micro-propagation of spine gourd in India or in neighbouring countries. In view of the above the research work on the development of large scale propagation of planting material of spine gourd employing the tissue culture has been developed at PRR Biotech Innovations Pvt. Ltd. This micropropagation procedure could be useful for raising genetically uniform planting material of known sex for commercial cultivation or build-up of plant material of a specific sex-type. Multiple shoots were induced from nodal, shoot tip and axillary bud of Momordica dioica Roxb. The maximum number of multiple shoots was obtained from MS medium containing BAP (2.0 mg L -1) and KIN (1.0 mg L -1) for nodal explants, BAP (1.0 mg L -1) combined with NAA (0.5 mg L -1) for shoot tip and BAP (2.0 mg L -1) with NAA (0.5 mg L International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 32 -1) for axillary buds. Maximum elongation of shoots from nodal, shoot tip and axillary bud explants occurred on MS medium augmented with BAP at 0.75 mg L -1 and 1.0 mg L -1 . Elongated shoots were harvested and transferred to MS medium containing IBA (1.0 mg L 1) for root induction. Rooted plants were acclimatized and further successfully transferred to the field. Abbreviations: BAP-N 6 -benzyl amino purine, IBA -indole-3-butyric acid, KIN -kinetin, MS medium -Murashige and Skoog (1962), NAA -naphthalene acetic acid. In vitro regeneration from different explants of Costus pictus - an insulin plant G.Sulakshana* and A.Sabitha Rani Department of Botany, Osmania University College for Women, Koti, Hyd. *Email: sulakshanasagar@gmail.com Costus pictus (Family:Costaceae) is an important medicinal plant widely used in traditional and modern system of medicine. The rhizomes contain diosgenin a steroidal saponin which is commonly used in treating diabetes. There is an increased demand for diosgenin production from Costus. Thus indiscriminate collection of these plants from the natural habitats is leading to their depletion. Therefore there is a need to conserve and propagate these plants. The present study was taken up to standardize in vitro regeneration of Costus pictus using different explants (mature leaves, young leaves, nodal and rhizome segments). The explants were cultured on SH media supplemented with different concentrations and combination of hormones like BAP, IBA, IAA, NAA and KN to inducing direct regeneration. Shoot buds appeared within 15-20 days from nodal and rhizome segments, whereas mature and young leaves did not respond for regeneration. Among all the combinations of hormones used, highest shoot regeneration (78.2%) was observed from nodal explants in SH media with 1 mg/l BAP+ 1 mg/l IBA. The rhizome segments exhibited moderate regeneration of 53.3%. Therefore nodal explants were taken for in vitro regeneration. To induce rooting, regenerated shoots were transferred onto SH media containing different concentrations of IAA and IBA. Among the two hormones, IBA 1 mg/l showed good rooting (63%) compared with IAA (40%).The regenerated plants were then transferred to the field with 92% survival rate. Study of Ethnomedicinal Plants of Bheemgal region of Nizamabad District T.Uma kiran 1 and M.Chandra kumar 2 1.Department of Botany, Girraj Government College, Nizamabad. 2. Department of Zoology, Government Degree College, Nirmal. Ethnobotany is the study of the utilitarian relationship between human beings and vegetation in their environment including medicinal uses. Indian subcontinent is known for its rich flora and fauna. The knowledge of modern plants has been accumulated in the course of many centuries based on different medicinal system like Ayurveda, Siddha and Unani. Since pre-historic times, plants and their extracts have been used for their healing properties. Our present investigation focused primarily on how plants are used in medicine and construction. The data is collected through personal interviews with village dwellers, traditional healers, and from herbal medicinal practitioners. The identified plants were documented for treatment of various ailments like skin diseases, kidney stones, antihelminthic, constipation, asthma and bronchitis etc. Medicinal Value of Rice Types for Human Health in India – A Review G. Praveen Kumar, A. Poshadri*, N. Sandhyakishore and B. Joseph. Regional Sugarcane and Rice Research Station, Rudrur, Nizamabad. *College of Food Science & Technology Professor Jayashankar Telangana State Agricultural University, Hyderabad, Telangana. Rice (Oryza sativa L.) is a “Global Grain” grown in different eco- geographical regions of the world and feeds more than 3 billion peoples. Today the world is suffering with chronic disease due to deficiency of vital nutrients in the diets. In order to overcome this problem, International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 33 rice supplemented with micronutrients is an important substitute for human malnutrition. In india, the tribal people and small farmers of the remote areas still growing indigenous rice varieties having nutritional and medicinal values. There are various types of Indian rice like White rice, Brown rice, Red rice, sticky rice, Black rice, Basmathi rice, Jasmine rice etc., that are healthy and contains vital nutritients. The paper is focused on types of rice and their nutritonal imporatnce. The White rice is the easiest food to digest at any time of the day and it helps in relieving digestive disorders such as diarrhoea, dysentery, colitis and even morning sickness. Brown Rice has less starch, less calories and an excellent source of soluble fibre. The oil present in this rice is good to reduce bad cholesterol. Red Rice regulates blood sugar and insulin. It contains vitamin B6, which is important to balance the formation of serotonin and red blood cells. Black rice is helpful for patients who are suffering from Alzheimer's and diabetes. Basmati Rice has a low to medium glycaemic index. It means that the energy released is slower and comes at a steadier rate leading to a more balanced level of energy and it contains fibre also. Plant Tissue Culture Studies on Coccinia indica-A Vegetable Crop Plant M. Venkateshwarlu(1) T.Ugendher(3) D.Srinivas(4)M.Nagaraju(1) P.Sreevani (2)and B.Mallaiah(1) (1)Department of Botany, Kakatiya University, Warangal – 506009 T.S. India (2)Department of Physics, Kakatiya University, Warangal – 506009 T.S. India (3)Department of Botany, S.R.R. PG College, Karimnagar – T.S. India (4)Department of Botany, Telangana University, Nizamabad–T.S. India drvenkat6666@gmail.com Plant tissue culture methods were applied for micro propagation of this medicinal important plant. The study established reliable and reproducible protocol for rapid multiple shoots induction from stem node explants of Coccinica indica. Using different concentration and combination of cytokinins. A reliable regeneration system culminating in the formation of complete from the mature stem nodes segments having 4–6 nodes. After initial swelling a maximum of 6 – 10 shoot buds were obtained on MS medium supplemented with a combination of Kn and BAP moreover like Adenine sulphate, ascorbic acid. Citric acid and glutamine were also found to be beneficial when incorporated in the above mentioned medium sholls was obtained on a separate media comprising of half the concentration of M.S. inorganic salts and high concentration of IAA, BAP, Kn, plantlets thus obtained were hardened acclimatized and finally transferred successfully to field conditions. The percentage of explants exhibiting shoot induction was found to be between 50-60 i. most of the concentrations of Benzyl amino purine. Several workers in past have micro propagated. Some of the important Asclepiadaceae members such as Ceroegia bulbosa (Britto et al, 2003).. Murashige and Skoog (1962) medium supplemented with 0.5 to 2.0 mg/l BAP was found to be optimum to induce shoots directly from the stem node explants. The present study was to develop a rapid system for regenerating shoots from mature stem node explants of Coccinica indica. Since very scarce information is available about micro propagation of this important vegetable crop plant, an attempt was made to develop a reproducible protocol for multiple shoot induction form stem node explants of one the culture. Study of Medicinal Values and Health benefits of Mustard Plant P. Latha1 and B. VidyaVardhini*2 1 Lecturer, Govt. Degree College, Armoor, Nizamabad, Telangana State 2 * Professor and Head, Dept. of Botany, Telangana University, Dichpally-503322, Nizamabad Email: latharajeshwar@yahoo.com Mustard (Brassica junceae) is an oil yielding plant belonging to the family Brassicaceae which is grown throughout the world. In India the Brassica crops occupy the second largest position after groundnut, with 3-5 million hectares, producing about 2 Million Tonnes of seed International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 34 annually. Mustard greens are easy to grow and have become a gardening favorite. This delicious and nutritious vegetables crop can be grown for most of the spring, summer and fall. With a little care you can enjoy this crop for most of the year.Plants are important sources of vitamins, minerals and flavonoids which are useful for maintaining good health of human beings. The seeds can also be pressed to make mustard oil and the edible leaves can be eaten as mustard greens. Mustard is a pungent winter vegetable plant, abundant when other vegetables plants are not in season. Mustard greens found to have more vitamin A, carotenes, vitamin K, and flavonoid, anti-oxidants than some of typical fruits and vegetables. Mustard and its oil have been used as a topical treatment for rheumatism and arthritis, as a foot bath for aching feet, and in the form of plasters over the back and chest to treat bronchitis and pneumonia. The characteristic pungent flavor of mustard oil is due to allyl isothiocyanate. Mustard oil has about 60% monounsaturated fatty acids (42% erucic acid and 12% oleic acid); it has about 21% polyunsaturated fats (6% the omega-3 alpha-linolenic acid and 15% the omega-6 linoleic acid), and it has about 12% saturated fats. Internally, mustard seeds have been used as appetite stimulants, emetics and diuretics. This paper studies the medicinal values of Mustard plant for obtaining health benefits to the human beings. Keywords: Mustard Plant, Medicinal Values, Vitamins, Flavonoids, Health Benefits Plants of Adilabad Forest used by Hakim for the Treatment of Certain Diseases Shaik Hafeez and J.Chapla Department of Botany, University College of Science, Osmania University, Hyderabad. Adilabad forest occupies about 7219 sq.km. The total population of tribals are 4, 17,000 (approximately). The present study was carried out recently. Practical work of administering medicines for Snakebites, Bone setting and Paranochiya disease and cure were recorded from the effected persons with the help of local Hakim. The forest of Adilabad is an excellent example for plant bio-diversity with a very good number of medicinal plants. The tribal people and the surrounding villagers of Adilabad forest mainly depend on the local hakim for the treatment of their diseases. Different snake bites have different medicinal plants for the treatment, different symptoms, different dosages and different timings, for example Naja Naja and Russell Viper. The same is the case for Bone setting and paranochiya. The hakim treats the patients free of cost. Hence the hakim commands lot of respect and regards. Antibacterial Activity in Methanolic Leaves Extract of Madhuca indica L. Yahya Khan and Sahera Nasreen P.G. Department of Botany, Government Institute of Science and Research Center, Nipat Niranjan Nagar, Caves Road, Aurangabad -431004 (M.S.) Corresponding author Email id – yahyakhan28@gmail.com Abstract Anti-bacterial activity of methanolic extracts of Madhuca indica was screened by agar Disc diffusion method. The results revealed that the methanolic extract exhibited significant antimicrobial activity of concentration of 100, 250, 500, 1000 µg/ml respectively against tested organisms, particularly more effective against gram – ve bacteria staphylococcus aureus and gram –ve bacteria Escherichia coli than the aqueous extract when compared to the standard drug (streptomycin). Keywords- Madhuca indica, antimicrobial activity S. aureus, E. coli. IntroductionMadhuca indica belonging to family Sapotaceae is an important economic tree growing throughout India. Traditionally Madhuca indica.Leaves have been used as an Anti-diabetic, Rheumatism, Ulcers, Bleedings and Tonsillits. The flowers, seeds and seed oil of Madhuca have great medicinal value. Externally, the seed oil massage is very effective to alleviate pain. In skin diseases, the juice of flowers is rubbed for oleation. It is also beneficial as a nasya (nasal drops) in diseases of the head due to pitta, like sinusitis. The purpose of the International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 35 present study was to evaluate the in vitro antibacterial activity of the methanol leaves extract of Madhuca indica.The external applications are skin effections, analgesic anti pyretic,antioxidant and anti diabetic. MATERIALS AND METHODS Plant materialDisease free leaves were collected from the campus of Government Institute of Science, Aurangabad. The collected leaves were surface sterilized with 0.1% mercuric chloride & then washed with D/W 2-3 times separately & shade dried. Fine powder were made after complete drying and used for the experimental work. Solvent Extraction of Leaves – Extracts were made in 80% methanol at room temperature by simple extraction method (Deshpande et al). 10 gm dried powder of leavesmixed with 100ml solvent in 250 ml flask and were kept on shaker for 24 hrs. Then it was allowed to stand for the 30 min to stand the plant material. Thereafter it was filtered & centrifuged at 5000 rpm for 15 min .The supernatant was collected &solvent was evaporated at 45 0C in vacuum evaporator to make the final volume 1/5 of the original volume Determination of Antibacterial activity Culture media – For antibacterial test Nutrient Agar/broth was purchased from Hi-Media Pvt. Ltd Bombay, India. Inoculums Preparation –The bacteria were inoculated into Nutrient broth &Incubated at37oC for 18 hrs & suspension was checked to provide approximately 3x 10 5 cfu/ml. Microorganism Used – The pure culture of test microorganism Bacillus cereus, E.coli. Staphylococcus aures, P.auroginosa was isolated from different samples in the Dept. of Botany Government Institute of science, Aurangabad (M.S.) Antibacterial assayThe Antibacterial activity of methanolic extract analysed by using disc diffusion assay (Dugler and Gomez, 2004) sterile disc was used for the present investigation. The extracts were incorporated to the sterile disc individually with 100, 250, 500, and 1000 µl using micropipette. Precautions were taken to prevent the flow of solvent extract from the disc outer surface .commercial antibiotic streptomycin (20 ml) was used for the positive control .The Disc was placed on the nutrient agar plates in which the bacteria were inoculated and spread uniformly and incubated at 37 oC+ 1oC for 24 hrs. The diameter of zone of inhibition was measured in mm. Table 3-Antimicrobial activity of methanolic extract of leaves of Madhuca indica S.No. Test Microorganism 1 2 3 E.coli. S.aureus B. cereus 4 P. auroginosa Zone of inhibition(mm) Conc. of methanolic leaf extract( µl ) 100 + +++ +++ + 250 + +++ +++ + Zone of inhibition (mm) Streptomycin(µl) 500 ++ +++ +++ 1000 +++ ++++ ++++ 400 +++++ +++++ +++++ ++ +++ +++++ International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 36 Plate 1: Antimicrobial activity of methanolic extract of leaves of Madhuca indica RESULT AND DISCUSSION Alcoholic extracts of leaves of Madhuca indica, were screened for antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and at dose level ranging from 100 µl/ml, 250 µl/ml, 500 µl/ml and 1000 µl/ml. (Table 1) methanolic extracts of Madhuca indica, leaves inhibited all the bacterial strains tested. All the doses (100 µg/ml to 1000 µg/ml) showed zone of inhibition against all the bacteria, even at a dose of 100µg/ml of extract exhibited significant zone of inhibition comparable to standard antibiotic (streptomycin) against S. aureus. For leaf extract B. cereus exhibited maximum inhibition (18 mm) which was greater than that of standard. For all other bacteria 100 µg/ml concentration of the extract was sufficient to produce effective inhibition. Concentration which were comparable to standard antifungal agent Streptomycin. Thus leaves of alcoholic extracts of Madhuca indica, were found to be inhibitory against all the bacteria tested. Antibacterial activities of alcoholic extracts of leaves of Madhuca indica, could be attributed to the presence of biological compounds like 2-Furan methanol, 4H pyran 4-one, 2,3-dihydro 3,5-dihydroxy-6-methyl,Thiophene, 2-Furancarboxyaldehyde-5-(hydroxymethyl) and 1,4tetra decanediol .The use of medicinal plants play a vital role in covering the basic health International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 37 needs in developing countries and these plants may offer a new sources of antibacterial, antifungal and antiviral agents with significant activity against inflective microorganisms. CONCLUSION The present study indicates that Madhuca indica, extracts have broad inhibitory activities to pathogenic microorganism and to act as potential antibacterial agent from natural sources. In general, commercial antibiotic and antifungal drugs causes side effects such as liver, kidney and gastrointestinal tract toxicity. Severe hepatotoxicity had also been reported in patients undergoing antifungal drug therapy. However, herbal remedies often do not produce any side effects. Therefore, alternative medicine become popular remedy to various types of ailments In conclusion, Madhuca indica extracts have revealed significant antibacterial activities against test organisms used for the study. Impact of Sulphur dioxide on medicinal value of Cassiatora T. Prasannarani1 , D. U Gawai2 1 Dept. of Botany, Lal bahadur shastri Mahavidyalaya, Dharmabad (M.S) 2 Dept. of Botany, Science College Nanded (M.S) Abstract: Cassia tora L. is popularly known for its medicinal value and various chemicals it possess. It is useful in treatment of fever, skin diseases, and acts as nerve tonic, acts as substitute for coffee and many more properties. But in nature being stationary and continuously exposed to polluted atmosphere particularly Sulphur dioxide there is tremendous effect on morphology and growth causing ultimately impact on its medicinal properties. In the present study an attempt has been made to understand the effect of different concentrations of Sulphur dioxide (10, 20, 30, 40 and 50 ppm) on the growth and morphological characters of Cassia tora seedlings. At the end of 15 days the effects were recorded in terms of colour and nature of leaves, stem, lamina, and dry weight of the plant. As the concentration of SO 2 increased (50ppm) different notable changes were recorded like change in the turgidity and inward curling of the leaves. Dry weight of the plant decreased with increase in the concentration of SO2 where as no change was noted in the nature of roots. I. Introduction A wide array of toxic substances including gases and particulates are emitted from the industries, especially from large industrial complexes, which affect our food supplies, health and economy. In particular these atmospheric pollutants adversely affect the growth and yield of practically all crop plants and structures of natural ecosystem. In India SO 2 has been recognized as a major air pollutant in urban and industrial areas (Chakraborty and Rao., 1962; Aiyanger et al., 1973; Yennwar et al., 1970). SO 2 not only affect well being of man kind, animals but also vegetation inclucing many medicinal plants (Rao et al., 1977). Cassia tora L. is one of the medicinal plant which posses various chemical. It is useful in treatment of fever, skin diseases, acts as nerve tonic, acts as substitute for coffee and many more medicinal properties. The effects of air pollution on the morphology, physiology and biochemistry of plants have long been recognized (Koziol and Whatley, 1984; Treshow, 1985; Ahmed et al., 1988). The leaves of the plants are highly exposed organs and they express most of the obvious effects. The present investigation deals with the effects of SO 2 on the growth and morphological characters like nature of leaves, nature of stem, nature of roots, and leaf lamina of Cassia tora L seedlings. II. Materials and Methods The seeds of Cassia tora were collected from Marathwada Agriculture University, Parbhani. Earthen pots of 25 cm2 diameter were filled with adequately fertilized sterilized black cotton soil and each to 15 seeds were sown. The thinning was done at the age of 10 days and only 10 plants were allowed to grow up to the age of 15 days. Pots were irrigated as and when required. The pots were exposed to different concentrations of SO 2 (10, 20, 30, 40 and 50 International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 38 ppm) was generated by following Khan and Kulshreshtra, 1991). The seedlings of Cassia tora L having the age of 21 days were placed in exposure chamber at different concentration for 6 hrs duration. After completion of the desired exposures of seedling, morphological changes were observed. III. Observations Effect of SO 2 on morphological changes of seedlings of Cassia tora L. SO2 ppm (v/v) Control 1 2 3 4 5 Nature of Leaves Nature of stem Nature of roots Nature of Lamina Dry weight (gm) 3.18 No change No change No change No change Two bottom leaves turned to pale yellow Older leaves turned pale yellow. Leaves became leathery. Leaves near to roots pale yellow. Leaves near to the roots are yellow. Stem turned pale yellowish green No change Old leaf lamina edge Brittle / Dried, Green coloured. 3.12 Pale yellow colour loss of rigidity No change Edge of Old leaf lamina was Brittle 2.83 Pale yellow colour Weaker & loss of turgidity No change Leaf lamina curling inward with Brittleness/Dryness. 2.55 Yellow coloured. Loss of turgidity No change 2.31 Older leaves are yellow in colour. Stem was pale yellow. It is highly weak with loss of turgidity. No change Leaf lamina curling inward with Brittleness/Dryness and total leaves of seedlings shows such symptoms. Leaf lamina Dry & Brittle to touch. 2.01 Results and Discussion In the present investigations growth and morphological changes of Cassia tora were observed at different concentrations of SO 2. It is evident from the results presented in table that, the seedlings of Cassia tora L. showed the following changes at the end of 15 days. The two leaves turned pale yellow at lower concentration of SO 2 (10 ppm) where as older leaves turned yellow at higher concentration (50 ppm). The Lamina of older leaves became Brittle/Dried at edge at lower concentration (10 ppm) SO 2, while at higher concentration (50 ppm) of SO2 all the leaves of seedling showed such symptoms along with inward curling of leaves. Stem was observed Pale yellowish green at lower concentration (10 ppm) while it became yellow, weak with loss of turgidity at higher concentration. No change was recorded in nature of roots. According to Halbwachs, 1984, very young or not yet fully expanded leaves, are relatively resistant and fully expanded leaves are more sensitive than the older ones. It is also evident from table that the dry weight of seedling decreased with increase in concentration of SO2. Similar kind of results were observed by Ayer and Bedi (1990) when maize plants were exposed in fumigation chamber with different concentration of SO 2. In their study, no of leaves, leaf area, biochemical parameters and yield was recorded maximum in 1.0 ppm SO2 exposure. In case of long term exposure to low SO2 concentrations the older leaves of Betavulgaris, Viciafabamalus and pyrus showed injury symptoms usually earlier then the younger ones (Guderian 1977). In conclusion, our study shows that change in the nature of leaves of Cassiatora due to SO2 can affect the functional contents there by altering its medicinal properties. IV. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 39 Medicinal plants used to cure amoebiasis in Khammam District of Telangana, India Dr. Ratna Manjula, R. Lecturer in Botany, Government Degree College, Rammannapet, Nalgonda District, Email: rratnamanjula1973@gmail.com Abstract The present study yielded 18 species belonging to 17 genera and 17 families used for curing Amoebiasis by the aborigines of the district. Symptoms of amoebiasis are usually gastrointestinal including diarreah, vo,iting, abdominal pain or discomfort and fever. There are eight herbs, six trees, two shrubs. CAESALPINIACEAE family is dominant family with 2 plants followed by Acanthaceae, Araceae, Asclepiadaceae, Bombacaceae, Combretaceae, Dilleniaceae, Euphorbiaceae,Flacourtiaceae, Hypoxidaceae, Lythraceae, Musaceae, Myrtaceae, Piperaceae, Punicaceae, Rutaceae, Sapotaceaewith each one. Of all the 18 practices, 16 practices involved one plant only, two practices involved two plants. Keywords: Ethnomedicine, Amoebiasis, Khammam district, Telangana INTRODUCTION Traditional herbal therapy is an age old practice (Rawat & Chaudhury 1998). This has cured varied diseases in the past and is still a favorite way out for the indigenous tribe. In fact, the traditional healing practices are arousing curiosity among various researches from all round the professions to go in depth into this subject (Tag et al 2005). Ancient traditional treatment methodology earns fame from its ethnic tribes, who still believe that traditional methods of application in curing many incurable diseases where modern medicine definite limitations. For the forest dwelling groups, age old practice of application plant-drugs for bone fracture, jaundice, pneumonia, diabetes, etc are still in demand to their modern counter pant (i.e. allopathic/ modern medicine). The local made herbal treatments along with enchantment for fast recovery by their local doctors are more sought after by the local tribes (Kala 2005). The Study Area Location, Topography and Geomorphology Khammam district came into existence on October 1, 1953. It was carved out from the taluks of Warangal and East Godavari districts and occupies an area of 16,029 km2 covering 46 Mandal Praja Parishads. It lies between 16° 45' and 18° 35' North latitude and between 79° 47' and 80° 47' East longitude. The total population of the district is 25, 78, 927 of which 6, 82,617 (26.46%) are scheduled tribes as per 2001 census. The district presents a rough topography with dissected uplands and hills, which sometimes exceeds 600 m. Temperature varies from 10 to 44° C. The average rainfall of the district is 1045 mm. The main tribes of the district are Koyas, Gonds/Naikpods, Lambadas and KondaReddis. The district has more than 52.6% forest land with 4 divisions. Dry deciduous, moist deciduous, riparian, scrub and grass land forest types are predominant. Though Filarial disease is important diseases exclusive studies on it are not many, necessitating the present investigation in Khammam district of Telangana state. METHODOLOGY An ethnobotanical survey was conducted during 2008-11 among the tribal communities of the district. Elder people, medicine men, tribal physicians and village old mothers were consulted to record first-hand information on ethnomedicinal uses, methods of preparation and administration of crude drugs. The information from the tribal people was compared with literature. Enumeration Alocasia indica Schott ARACEAE VN: Enugu chevi aku E: Large leaved caladium, Gaint taro Stout coarse herbs; leaves 2-4 feet long, deeply cordate, basal lobes ovate; petioles long and stout; spathe stalked; flowers unisexual; spadix free; perianth 0; stamens 3 -8, International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 40 connate into a synandrium; ovary unicarpellary, unilocular; ovules few; style very short; stigmas 2-4 lobed; berries obconical or ellipsoid; seeds subglobose. Fl & Fr: Jun-Nov Wild Leaf decoction mixed with equal quantities of Cuminum cyminum and Foeniculam vulgare is administered in 3 spoonful daily twice 3-5 d. Cassia auriculata L.CAESALPINIACEAE VN: Tangedu S: Avartaki H: Taswar E: Tanners cassia Shrub; branchlets finely pubescent; leaves long, leaftets 8-12 pairs, glands between leaflets; corymbs axillary and terminal, often panicled; petals yellow, veined; pod flat, dehiscent, obtuse. Fl&Fr: Nov-May Wild. Three spoonful of stem decoction is administered twice a day for 3 d. Cassia italica (Mill.) Lam. ex Andr. CAESALPINIACEAE VN: Sunamuki E: Country senna Deffuse herb or undershrubs; leaves pinnate; leaflets 4-8 pairs, obovate; ellipticoblong; foliar glands absent; flowers yellow, in axillary racemes; stamens 10; pods curved. Fl & Fr: Oct-May Wild. Stem with that of Ficus hispida made into paste is administered in 2 spoonful twice a day till cure. Ceiba pentandra (L.) Gaertn. BOMBACACEAE VN: Tella buruga S: Swetha salmali H: Safed samul E: White silk cotton tree Tall tree, trunk straight, prickly when young, branches horizontal; leaves 6-9 foliolate, leaflets oblanceolate or elliptic or oblong; flowers clustered at the ends of branches, white or yellowish; fruit ellipsoidal capsule, seeds many. Fl & Fr: Jan – May Wild and cultivated. Stem bark decoction is taken in one spoonful twice a day. Ceropegia junceaRoxb. ASCLEPIADACEAE VN: Bellagadda E: Leafless goglet flower Twining herb; stems fleshy; leaves decidous; flowers brownish-purple, solitary or in cymes, axillary; sepals and petals 5 each; pollinia oblong; follicles cylindric (Fig. 65). Fl & Fr: Aug-Feb Wild. Five ml of root decoction mixed with a cup of curd is taken once a day till cure. Curculigo orchioides Gaertn.HYPOXIDACEAE VN: Nela tadi S: Musali tala mulika H: Kala Musali E: Black musali A small herb with corm, root stock elongate; leaves lanceolate narrow, flowers yellow, solitary, perianth lobes 6, stamens 6, ovary hidden among the leaves, 3 celled, stigmas 3; fruit somewhat succulent; seeds globose. Fl&Fr: Jul-Oct Wild. Five g of tuber powder mixed with a cup of curd is administered twice a day for 3 d. Dillenia indicaL. DILLENIACEAE VN: Kalinga chettu, Revadachettu S: Korhotta H: Chalta E: Elephant apple A medium to large sized tree; branches spreading; leaves sharply serrate; flowers creamy; sepals thick; fruit fleshy enclosed in accrescent fleshy sepals; seeds compressed, embedded in glutinous pulp. Fl: May-Jul Fr: Mar-Apr (next year) Wild. Ten g of young fruits are boiled and eaten. Flacourtia indica (Burm. f.)Merr.FLACOURTIACEAE VN: Kana regu S: Aghori H: Kancu E: Governor’s plum International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 41 A small tree; branchlets sparsely armed; leaves simple, oblong, orbicular or obovate, cuneate at base, crenulate, retuse at apex; flowers pale yellow, in racemes; fruits globose, dark purple drupes with juicy pulpy and hard endocap. Fl & Fr: Jan – Jun Wild. Two spoonful of leaf juice mixed with half spoon of dried ginger powder is administered twice a day for 3 d. Lepidogathis cristata Willd. ACANTHACEAE VN: Nakka pitha gadda E: Cristata Herbs with stout rootstocks; leaves linear-oblong; flowers white, in globose spikes; clustered at the base of the stem. Fl & Ft: Nov – Mar Wild. Thirty ml of whole plant decoction is taken once for 3 d. Mimusops elengi L.SAPOTACEAE VN: Pogada S: Bakula H: Mulsari E: Spanish cherry Medium sized evergreen tree, bark scaly; leaves variable, elliptic-oblong, tip acuminate, margin entire, coriaceous, base truncate to acute; flowers solitary or few in fascicles of 2-6; fruit ovoid berry. Fl & Fr: Mar – May Wild and cultivated. Two spoonful of fruit pulp mixed with one spoon of sugar is taken once a day for 3 d. Musa ornata Roxb. MUSACEAE VN: Adavi arati E: Wild banana Herbs; leaves broadly elliptic; inflorescence erect; peduncle glabrous; flowers of the basal bracts uniseriate, female 6-flowered, lotus pink; flowers of the upper bracts male; ovary oblong, stigma capitate; staminodes 5; male flowers uniseriate; fruits yellowish when ripe; seeds numerous, black. Fl & Fr: Aug-Nov Wild. Young shoots are made into juice and administered in 2 spoonful once a day till cure. Naringi crenulata (Roxb.)Nicol. RUTACEAE VN: Torri velaga E: Dog’s wood apple Deciduous thorny trees; spines axillary, straight; leaves in clusters; leaflets 3 pairs, ovate or elliptic; flowers 4 or 5 merous, white, in axillary racemes; berries globose; seeds 3 or 4, ovoid. Fl & Fr: Apr-Sept Wild. Thirty ml of stem decoction mixed with a cup of milk is administered once a day till cure. Phyllanthus emblica L. EUPHORBIACEAE VN: Usiri S: Amalaki H: Amla E: Indian gooseberry A deciduous tree, branchlets reddish brown, villous; leaves linear, apiculate, pinnately on the branchlets, stipules scarious; flowers minute, monoecious, in axillary fascicles, tepals 6, stamens 3, ovary 3 celled, fruit drupe, green, globose. Fl & Fr: Jun-Mar Cultivated. Twenty ml of leaf juice mixed with half spoon of seeds of trigonella is administered twice a day for 3 d. Piper nigrum L.PIPERACEAE VN: Miriyalu S: Maricha H: Kali-mirch E: Black pepper A stout glabrous climbing perennial, rooting at the nodes; leaves simple, alternate, cordate, very variable in breadth, broadly ovate; flowers minute, bisexual, in axillary spikes; fruiting spikes very variable in length, fruits ovoid or globose, 1-seeded berries, black when mature. Fl & Fr: Jun – Apr Cultivated. One spoon of seed powder is taken with one spoon of buttermilk once a day till cure. Punica granatum L. PUNICACEAE International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 42 VN: Dhanimma S: Dadimasara H: Anar E: Pomegranate A glabrous, deciduous, small tree, branchlets often spine tipped; leaves oblanceolate or oblong; flowers bright-red, solitary or 2-3 together, terminal; fruit globose berry, crowned by persistent calyx-lobes, interior separate with membranous wall, pulp pellucid, pinkish. Fl & Fr: May – DecCultivated. Half cup of fruit juice is taken twice a day till cure. Syzygium cumini L. MYRTACEAE VN: Adavii jinne, Neredu S: Jambuh H: Jambhal E: Indian cherry Tree with grey bark, exfoliating; leaves coriaceous, elliptic, acuminate, shining; flowers tetramerous, subsessile in trichotomous lateral panicles; petals united into calyptra and falling off in one piece; berries ovoid or oblong, dark purple, juicy; 1 seeded. Fl&Fr: Mar-Aug Wild and cultivated. One spoonful of stem bark decoction is administered daily twice till cure. Terminalia bellirica(Gaertn.)Roxb.COMBRETACEAE VN: Tani S: Vibhutak H: Bahera E: Belleric myrobalan A large deciduous tree; leaves long petioled, densely clustered at the ends of branchlets, elliptic-oblong, acute, coriaceous; flowers pale yellow in axillary spikes, upper flowers male, lower ones bisexual; fruits sub-globose clothed with fine rusty felt (Fig. 130). FI & Fr: Mar – May Wild and cultivated. Gum is ground and the powder is administered in 10 g dose along with a cup of cow milk. Woodfordia fruticosa (L.) Kurz LYTHRACEAE VN: Adavidraksha, Jujuki S: Dhataki H: Dhai E: Fire flame bush Large shrub, with spreading branches; leaves opposite, ovate-lanceolate, base rounded or cordate, margin entire, apex acuminate; flowers in axillary clusters on slender pedicels, corolla bright red; capsules oblong or ellipsoid, enclosed by persistent calyx tube, seeds minute, pale brown, numerous, smooth. Fl & Fr: Mar – Jun Wild. Stem bark decoction is administered in 3 spoonful daily once till cure. RESULTS AND DISCUSSION The present study yielded 18 species belonging to 17 genera and 17 families used for curing Amoebiasis by the aborigines of the district. There are eight herbs, six trees, two shrubs. Caesalpiniaceae family is dominant family with 2 plants followed by Acanthaceae, Araceae, Asclepiadaceae, Bombacaceae, Combretaceae, Dilleniaceae, Euphorbiaceae,Flacourtiaceae, Hypoxidaceae, Lythraceae, Musaceae, Myrtaceae, Piperaceae, Punicaceae, Rutaceae, Sapotaceae with each one. Of all the 18 practices, 16 practices involved one plant only, two practices involved two plants. Present investigation indicates that Khammam district is blessed with magnificent diversity of ethno-medicinal plants used to cure many diseases. The present study will give new incentive to the traditional system of healthcare. Further, this approach for the treatment of amoebiasis is a practical, cost-effective and biological safe. Medicinal plants used in the treatment of Diabetes by ethnic people of Nizamabad district, Telangana, India J. Vasudev and B. Vidya Vardhini* Department of Botany, Telangana University, Dichpally, Nizamabad -503322, India E-mail id: drvidyavardhini@rediffmail.com Abstract An attempt has been made to compile the ethno botanical utilization of the data presented in the present study which has brought into light around 25 species of medicinal plants which are used by the tribals for their health care as well as day to day life of different ethnic groups such as Kolams, Naikpods, Pardhans, Yerukalas and Lambada of Nizamabad district located International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 43 in the northern part of the state. The traditional knowledge regarding the use of these plants is widely applied by these ethnic groups. The diverse ethnic communities together with the luxurious floristic diversity offer ample scope for the ethno botanical study in this district. The indigenous groups depend either directly or indirectly on the products of the forest for their livelihood and have, down age ages, preserved the knowledge about the traditional and cultural uses of plants. Keywords: Ethno botany, Diabetes, Nizamabad district, Telangana Introduction Nizamabad district is situated in the northern part of the state Telangana. It lies between 18-5' and 19' of the northern latitudes, 77-40'and 78-37' of the eastern longitudes. The district is bounded on the North by Adilabad district, East by Karimnagar District, South by Medak district and West by Bidar District of Karnataka and Nanded district of Maharashtra. The geographical area is around 7956 Sq. km’s i.e. 19,80,586 acres spread over 923 villages in 36 mandals. Major rivers such as Godavari and Manjeera crosses Nizamabad district with some other streams Kalyani, Kaulas, Peddavagu also exist in the district. Forests: The forest is covering area of around 1.67 lacs hectares (4,18,450 acres) forming 22% of the total geographical area of the district. The forests fall under the category of Southern Tropical dry deciduous type. Thick forest belt produces major population of Dalbergia, Tectona, Terminalia, Rhynchosia species. The forest produce, which includes timber, fuel, bamboo and Diospyrosleaves yields good revenue. Mangoes and Custard apples grow well in the district. Forest Dwellers: As per 2011 census the total population of the district is 25.51 lakhs and of which these tribal population is 1.92 Lakhs. Lambada, Naikpod, Yerukalas etc. are major tribal groups in the area. Of these, Lambada is found most abundant throughout the area. Besides these tribal groups, several other communities are residing as forest dwellers. Since ancient times, plants have been an exemplary source of medicine. Ayurveda and other Indian literature mentioned the use of plants in treatment of various human ailments. In India, indigenous plant remedies have been used in the treatment of Diabetes mellitus since the time of Charaka and Sushruta (6 th century BC). Plants have always been an exemplary source of drugs and more than 80% of the currently available drugs have been derived directly or indirectly from them. Medicinal plants have the advantage of having no or only few side effects. Some of them are being used in traditional systems of medicine from hundreds of years in many countries of the world. Metformin is an oral antidiabetic drug used for the treatment of non-insulin-independent diabetes mellitus (NIDDM) patients. Metformin is now believed to be the most widely prescribed antidiabetic drug in the world; it was first derived from a medicinal plant Galega officinalis, which was historically used for treatment of diabetes in medieval Europe. There are many antidiabetic plants which might provide useful sources for the development of drugs which can be used in the treatment of Diabetes mellitus. The literature on medicinal plants with antidiabetic activity is vast, so a few commonly used plants have been discussed here. Methodology For documentation of ethno-botanical information and collection of plant material, several tours were undertaken during the period 2014 - 2015. Data presented here is based on personal observations and interviews with traditional healers’ viz., medicine men, hakims and old aged people and the methodology used is based on the methods available in literature (Jain, 1989) and (Jain and Mudgal, 1999). Ethnobotanical information gathered was documented in datasheets prepared. For collection of plant material, local informer International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 44 accompanied the authors. Plant identification was done by using regional flora and flora of adjoining districts (Pullaih and Rao, 1995; Cooke, 1958). Medicinal uses of plants were compared with other major published literature available (Ambasta, 1992; Anonymous, 1948-1976; Asolkar et al., 1956; 1969; Jain 1991; Jain, 1996; 1999; Kapur 2001; Kirtikar & Basu, 1993; Pradhan et al.,2005; Reddy et al.,2007 ; 2008; Reddy, 2008; Sharma & Singh, 2001). Enumeration The present ethno-botanical explorations conducted in forest areas of Nizamabad resulted in the traditional plant uses of 25 plants species belonging to 42 families. The following data includes botanical name of species, vernacular name, family, plant part used, method of preparation of medicine and mode of administration and details about its application. The name of the tribal group who provides the information is given in parenthesis after each use. In case of the informer being a non tribal, he has been put in category of forest dweller. Table-1 S.No Botanicalname Commonnames Part Used Beneficialeffects 1. Aloevera L. Kalabanda Family Liliaceae 2. Aegle marmelos L. Bilva, Patri Rutaceae Seeds 3. Amaranthuscaudatus L. Thotakura Amaranthaceae Leaf 4. Andrographis paniculata (Burm.F.) Wall ex Nees. Nelavemu Acanthaceae Leaf Hypotriglyceridemic effect, α-amylase enzyme inhibition 5. Boerhaaviadiffusa L. Nyctaginaceae Leaf 6. Azadirachtaindica L Atakamamidi, Punarnava Vepa Meliaceae Leaf Increases plasma insulin concentration andinsulinsensitivity Hypoglycemic , reduces peripheral utilization of glucose and glycogenolytic effect 7. Caesalpiniabonducella (L.) Roxb. Gachchakkay Caesalpiniaceae Seed Hypoglycemic, insulin secretagogue, hypolipidemicactivity 8. Capparis zeylanica L. Adonda Capparaceae Fruit, Leaf Hypoglycaemic 9. Cassia auriculata L. Leaf Catharanthus roseus (L.) G.Don Tangadu, Sonakuki Billagannaru, Chakram pullu Caesalpinaceae 10. Apocynaceae Leaf Hypoglycaemic properties and galactosidase inhibition α-amylase inhibition 11. Ficusbenghalensis L. Marri Moraceae Leaf Hypoglycemic, hypolipidemic, inhibits insulinase activity from liver and kidney, Insulinmimetic activity 12. Ficus hispida Linn.f Bommidi, Budda chettu Moraceae Fruit Hypoglycemicactivity 13. Glycyrrhizaeradix L. Athimadhuram Fabaceae Root Hypoglycemicactivity 14. Gymnemasylvestre (Retz). Madhunashini, R. Br. Ex Schult Poda patri Asclepidaceae Leaf Stimulation of repair or regeneration of beta cells, anti-hyperglycemic effect, hypolipidemic 15. Momordica charantia L. Kakara Cucurbitaceae Unripe fruit, Seeds Insulin mimetic activity 16. Ocimum sanctum L. Tulsi Lamiaceae Leaf 17. Phyllanthus emblica Gaertn. Usiri Phyllanthaceae Fruit Lower blood sugar level, potent hypoglycemic and hypolipemic effect Decreases lipid peroxidation, antioxidant, hypoglycemic 18. Punica granatum L. Danimma Punicaceae Flower, Peel Antioxidant, anti-hyperglycemic effect 19. Pterocarpus marsupium Roxb. Syzigium cumini L Vegisa, Peddagi Fabaceae Wood Hypoglycemic, insulinogenic-enhance insulin release Neredu, Allanaradu Nelavemu Myrtaceae Seeds Gentianaceae Leaf Hypoglycemic, anti-oxidant activityty, α-glucosidase inhibitory activity Stimulates insulin release from islets Combretaceae Fruits Hypoglycemic, antioxidant, hypolipidemic activity 20. 21. 22. Swertia chirayita (Roxb ex Flem) Terminalia bellerica Roxb. Tanikaya Leaf Hypoglycemic , wound healing indiabetics Anti hyperglycaemic activity along with decreas ed cholesterol and blood urea , increased plasactivity ma ins ulin level Hypoglycaemic International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in α- Proceedings of National Seminar on Medicinal Plants For Better Human Health 45 23. Terminalia chebula Retz. Karakkaya Combretaceae Fruit Hypoglycemic, antioxidant, hypolipidemic activity 24. Tinospora cordifolia (Willd). Hook.f Tippa teega Menispermaceae Leaf Anti-hyperglycemic, stimulates islets 25. Trigonella foenumgraecum L. Menthulu Leguminosae Seeds Hypoglycemic activity, stimulates insulin release by islet cells insulin release from Results and Discussion The present study encompasses the in-depth investigation on medicinal plants which are used in Ethnobotanical medicine by the local healers in the district of Nizamabad, Telangana. The majority of the rural people of the district population depend on livestock rearing and agriculture farming activities. Illiteracy, ignorance, superstition, taboos are common among the rural population. An attempt is made to gather information from the tribal communities such as Kolam, Naipod, pardhan, Lambada, Yerukalas. These communities are directly interlinked with nature and having a symbiotic relationship with the medicinal plants in the region. The author has recorded around 25 plants species belonging to 20 families of medicinal plants which are used by the herbal practitioners for treating health care applications. The detailed description of local medicinal uses along with local names, family, part used, botanical name and beneficial aspects are given (Table-1). Conclusion The present study indicates that the primary health care of the inhabitants is being taken care by local tribal communities. Healers never charge any fee for treatment but they ask farmers to bring additives like pepper, chillies, curcuma powder, jaggery etc., required for preparing medicine. Valuable herbal practices which are being practiced by tribal communities of Telangana State have to be studied scientifically and it is also very important to document and publicize the ethno medicinal plant knowledge among the young generations to raise awareness of and appreciation for traditional values. It also helps to conserve the sustainable use of the plants as well as to keep the traditional medical knowledge. The tribes living in and around the forest area are very much dependent on herbal practices due to lack of communication and cost of allopathy. A study on Ethno medicinal Values of Plants used by Koyas in Tadvai Mandal, Warangal District, Telangana, India K. Anuradha Department of Botany, Government Degree College, Siddipet, Medak Dist. E-mail:saiteja.b@gmail.com Abstract An Ethnobotanicalsurveywasconducted out among the ethnic groups (Koya)intheTadvai Mandal, Warangal District of Telangana. Koyas have rich k nowledge abou t medicinal pla nts and their ethno medicinal valu es. In this stu dy, it is observed that the Koya s u se 34plants belonging t o 26familiesused bytraditional healers.The medicinal plants were identified and documented in this paper with regard to their botanical name, family, local name, parts used and utilization by the local tribal people for different human ailments.This paper reports the uses of medicinal plants by the tribal people in the form of powders, extracts, decoctions, pastes, etc. Keywords:MedicinalPlants;Ethno medicine; W a r a n g a l district;Telangana; Traditional use. Introduction Plants have been used in traditional medicine for several thousand years. Now a days, study of medicinal plants and their utilization has been drawing the attention throughout world. People using herbal medicine because it is safer, without any side effects. For this reason the study of ethno medicine has become an important issue. Ethno medicine is the study of the beliefs and practices regarding illness in different human populations (Genest, 1978). In International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 46 India, it is reported that traditional healers use 2500 plant species and 100 species of plants serve as regular sources of medicine (Pei, 2001). During last few decades there has been an increasing in the study of medicinal plants and their traditional use in different parts of the world (Lev, 2006). Ethnic groups of various regions of the world are the real care takers of nature and experts in herbal therapy. The traditional ethnic knowledge of phytocure methods transferred orally for centuries is fast disappearing because of the developments and changes in the culture of tribal groups (Ganesan, et al 2004). Conventionally, this knowledge has been passed on orally from generation to generation without any written document (Perumal Samy and Ignacimuthu, 2000) and is still retained by various indigenous groups around the world. But this knowledge is to be secured and conserved for future generations (Burmol & Naidu, 2007). The traditional systems of medicine that have evolved over the centuries within various communities are still maintained as a great knowledge in herbal medicines (Mukherjee and Wahil, 2006).Documenting the indigenous knowledge through ethnobotanical studies is important for the conservation and utilization of biological resources. Ethnobotanical survey has been found to be one of the reliable approaches to drug discovery (Fabricant and Farnsworth, 2001). The main focus of the present study is to discover the detailed information on the use of plants and their healing practices among Koya tribes. Methodology Fieldtripswereconductedintribalpartsof Tadvai Mandal in Warangal district.Collectiondataincludedlocalname,plantpartusedandmodeofadministration. Atthetime,actualapplicationswerealsoobservedduringthefieldwork. Theethno medicalinformationwascollected through interviews and discussionsfromknowledgeablelocalagedpeople,herdsmenandlocal healers. Therepeatedvisitsto thevillageshelpedindevelopingintimacywiththelocalpeople.Thephytocureinformationwascolle ctedfrom Koyasthroughinterviewsanddiscussions.Thescientificnamesofplantspeciestheirfamilieswereid entifiedwiththe helpofforestfloraofHyderabadstate(SharfuddinKhan,M.1959)andfloras(Gamble&Fischer1915 ─1935). Results&Discussion Inthepresentaccount,34 speciesofangiospermsbelongingto 26familiesare reported. The parts of the plant used for medicinal purposes are leaves, root, stem, seeds the whole plant and barks. However, leaves were found most frequently used part. Theyareusedasethno medicinesforvariousseverediseaseslike dia betes,cancer,etc.Othercommondiseasesandhealthcomplaints likecough,piles,fever,bonefracture,stomachache,bloodpressure,dandruff and asthmaarecuredbyusingthe medicinal preparationsofvariousplants.T he mode of ethno medicine u sage for different diseases is in variou s forms, such as aqu eous extra cta nd paste. In addition, milk, ginger, pepper, and jaggery etc. are used as ingredients in preparation of ethno medicine (Sudhakar Reddy et al ., 2008). T he ethnic tribe (Koya) of these villages are healthy and not su ffering fr om common problems lik e depression, blood pressure and diabetes which are common in urban people (Ishwar Prasad et al., 2009).Thealphabeticalorderofscientificnameoftheplants,theirfamilieslocalnames,diseases,par tsused,mode ofadministrationwithdurationanddosesarefurnishedin(Table 1). S.N o 1 Name of the plant Family Local name Useful part Mode of Administration Abutilon indicum Malvaceae Thuthuru benda Leaf Leaf is cooked with onion and taken International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Disease treated Piles Proceedings of National Seminar on Medicinal Plants For Better Human Health 47 orally to treat piles 2 Acacia intsia Mimoseceae Korinta Root 3 Achyranthes aspera Amaranthaceae Uttareni Leaf 4 Amaranthus spinosus Amaranthaceae Mulla thotakura Leaf, Root Leaf The aqueous extract of root is administered twice a day for 7 seven days Leaf crushed and paste applied for eyes in 1 to 2 drops per day for 3 days Leaf, root paste along with lemon juice is taken daily Leaf extract taken orally in 2 spoon ful/day for Three days Leaf extract is poured in nostrils for curing cold. Leaf ground with sugar and given orally in 1 spoon thrice a day for 3 days 5 Adhatoda zeylanica Acanthaceae Adda saramu 6 Aegle marmelos Rutaceae Maredu Leaf 7 Andrographis paniculata Acanthaceae Nela vemu Leaf 8 Anogeissus latifolia Combretaceae Chiriman Gum 9 Argemone mexicana Papaveraceae 10 Boerhaavia diffusa Nyctaginaceae 11 Boerhavia erecta Nyctaginaceae Galijeru Leaf 12 Borassus flabellifer Palmaceae Tadi Root 13 Cassia auriculata Caesalpiniaceae Tangedu Leaf 14 Cassia tora Fabaceae Penta chennangi Shoot tip 15 Catharanthus roseus Apocyanaceae Billa ganneru Leaf 16 Chloroxylon swetenia Rutaceae Billu Bark 17 Cissus quadrangularis Vitaceae Nalleru Stem with Leaf 18 Coccinia grandis Cucurbitaceae Kaki donda Leaf 19 Cocculus hirsutus Menispermaceae Dushta teega Leaf 20 Crataeva magna Capparidaceae Vulimiri Chettu Leaf 21 Euphorbia hirta Euphorbiaceae Pacha botlu Whole plant 22 Ficus glomerata Moraceae Medi Bark 23 Gymnema Sylvestre Asclepiadaceae Poda Patri Whole plant 24 Hemidesmus indicus Asclepiadaceae Sugandha pala Leaf 25 Lantana whitiana Verbanaceae Puli kampa Leaf 26 Lawsonia inermis Lythraceae Maidaku Leaf 27 Leucas aspera Lamiaceae Tummi Leaf 28 Mimosa pudica Mimosaceae AttiPatti Leaf 29 Ocimum basilicum Lamiaceae Sabja Leaf Dried Leaf are kept in fire and the smoke is inhaled to cure Asthma 30 Plumbago Zeylanica Plumbaginaceae Chitra moolam Leaf leaf paste miledly heated and gently massaged Brahma dandi Atika mamidi Latex plant The gum is eaten for improve Potency. Latex of the plant is applied topically on the site of boils The plant is pasted with cumin and taken internally to cure digestive problems Leaf extract mixed with sugar and curd and administered early in the morning for 4 days The extracts of roots are orally given for krite bite. Leaf paste with egg albumen plastered over the bone parts till cure Tender twigs are cooked with onion and taken with food Leaf paste applied on tumors and the decoction given orally in 30ml twice a day for about 1 month The extract of bark in combination with pepper and cloves are orally administrated with a dose of two spoons for a day The paste of stem with Leaf is applied for healing fractures. Leaf extract administered in 2 spoonfuls twice a day for about 10 days Leaf juice administered in 2 spoon fuls with a glass of Sugar water twice a day for 10-15 days Leaf extract administered during the bleeding condition in 2 spoon fuls twice a day for 15 days, the paste applied externally as ointment Plant decoction used two spoonful twice a day for 15 days The aqueous extract of bark , pepper and cardmom is administered a spoonful per day Plant decoction administered in 2 spoonfuls twice a day for about 2 months Root and Leaf decoction is taken orally to regulate digestion The leaf is ground with Cipadessa baccifera root, leaf and bark & applied topically to treat Psoriasis Leaf paste applied on head Hair growth 1 hour before the bath for twice a week for 1 month Leaf paste or crushed leaf is taken both externally & internally to treat snake bite, It is also applied topically on the forehead to cure one side headache Root and Leaf infusion is applied on the wounds International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Cough Ophthalmic Problems Stomach ulcer Cough Cold Fever Dental Boils Digestive disorder Jaundice Snake bite Bone fracture Ophthalmic Problems Cancer Asthma Bone fracture Diabetes Gonorrhea Piles, fistula Branchial infection, asthama Constipation Diabetes Stomach disorder Skin disease Dandruff, Alopecia Snakebite, headache Wound healing Asthma, breathing problems Chest pain Proceedings of National Seminar on Medicinal Plants For Better Human Health 31 Sida acuta 32 Strychnos Vomica 33 34 48 Malvaceae Muttavapulagamu Leaf Loganiaceae Esha mushti Leaf Syzygium Cumini Myrtaceae Alla neredu Leaf Seeds Tridax procumbens Asteraceae Gaya paku Leaf nux - and Crush the fresh Leaf and the juice is applied topically to treat boils Leaf, ground with ginger and make into small pills,administered in 2 pills thrice a day for 10-15 days Leaf juice with Sugar water given to children, Seed Powder with water are taken orally in empty stomach Leaf juice is applied topically on wounds Boils Blood pressure Diarrhea, diabetics Wound healings Conclusion The data collected shows that majority of the remedies are taken orally. Most of the reported preparations are drawn from a single plant; mixtures are used rarely. The fresh plant parts are used for the preparation of medicine. When fresh plant parts are unavailable, dried parts are also used. Generally, the people of the study area still have a strong belief in the efficacy and success of herbal medicine. The results of the present study provide evidence that medicinal plants continue to play an important role in the healthcare system of this tribal community. This study provides an ethnobotanical data of the medicinal plants used by the tribal people of Koyas to cure different diseases. Moreover, this study will promote a practical use of botanicals and must be continued focusing on its pharmacological validation. Further detailed exploration and collection of ethnobotanical information, chemical studies and screening for medicinal properties will provide cost effective and reliable source of medicine for the welfare of humanity. Effect of Salicylic acid on the growth of Cotton grown in Nizamabad District Y. Venkateshwarlu,B. VidyaVardhini Department of Botany, Telangana University, Dichpally.Nizamabad. (TS) Phytohormones have an important role to play in mediating plant responses to abiotic stress. Plants have developed over the years a variety of physiological and biochemical mechanisms through which they survive under the stressful conditions. In the recent times, other phytohormones like brassinosteroids, methyl jasmonates, salicylic acid, and strigolactones have been shown not only to regulate plant growth and development but also to protect plants from various abiotic stresses like high temperature, low temperature, salt, high light, weak light, drought as well as flooding, heavy metals, herbicide stress, pesticide stress and even biotic stresses. Salicylic acid (SA) is a phenolic compound which, despite its broad distribution in plants, has basal levels differing widely among species, with up to 100-fold differences having been recorded. It plays a major role in the regulation of plant growth, development, and interaction with other organisms and defense responses to environmental stresses. Salicylic acid (SA) acts as endogenous signal molecule that is responsible for inducing abiotic stress tolerance in plants. Cotton (Gossypium herbaceum L.) is a commercially grown crop throughout India and is very responsive to environmental changes. Cotton is a fibre, oil and protein yielding crop grown in various parts of Telangana State of India. The ever changing environment is posing a severe threat on the growth and yield of cotton crop. The employment of growth promoting substances has been extensively used for the control of growth of cotton. In the present study, the effect of a plant growth regulator, salicylic acid was selected to investigate its effect on growth, yield and metabolism of two varieties of cotton (Bt and non Bt) varieties grown in semi-arid tropics of Nizamabad District of Telangana State in India. The present research is a field experiment where two varieties of cotton seeds (Bt and non-Bt) were sown in different rows in the field which was supplemented with farmyard manure. Plants were grown under natural day length. SA was supplied to the plants as foliar spray at three different concentration levels viz., 0.5 mM, 1.0 mM and 2.0 mM on 40th, 50thand 60th day (from the day of sowing). Growth parameters International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 49 were recorded on the 65 th day. Plant material was fixed on the 70 th day by homogenizing with 80% ethyl alcohol which was used for the estimation of the biochemical parameters. Medicinal Plants of Nizamabad District with anti-diabetic potential Gaddam Srinivas Reddy, T.Venugopala Swamy*, S.Prashanthi** Department of Zoology, Govt. Junior College for Boys, Nizamabad *Department of Zoology, Girraj Govt College, Nizamabad **Department of Botany, Telangana University Abstract Diabetes mellitus is one of the common metabolic disorders acquiring around 2.8% of the world's population and is anticipated to cross 5.4% by the year 2025.The present survey has been carried out to explore the medicinal plants of Nizamabad district in the treatment of diabetes. Medicinal plants are nature’s wonderful gift to mankind and are involved in India’s very rich and ancient heritage of traditional systems of medicine i.e Ayurveda, Sidda and Unani. The present paper reviews about 27 medicinal plants and their products available in Nizamabad district. Key words: Medicinal Plants, Ayurveda, Unani, Diabetes, Nizamabad, Telangana Introduction The World Health Organization has listed 21,000 plants, which are used for medicinal purposes around the world. Among these 2500 species are in India. India is the large producer of medicinal plants and is called as botanical garden of the world. The ethno botanical literature provides the information about 800 plants may possess anti-diabetic potential; however searching for new antidiabetic drugs from natural plants is still attractive because they contain substances which demonstrate alternative and safe effects on diabetes. Diabetes is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action or both. The chronic hyperglycemia of diabetes is associated with long term damage, dysfunction and failure of different organs especially the kidneys, eyes, nerves and heart. Several pathogenic processes are involved in development of diabetes. These range from autoimmune destruction of the β-cells of the pancreas with consequent insulin deficiency to abnormalities that result in resistance to insulin action. The basis of the abnormalities in carbohydrate, fat and protein metabolism in diabetes is deficient action of insulin on target tissues. Deficient insulin action results from inadequate insulin secretion and or diminished tissue responses to insulin at one or more points in the complex pathways of hormone action. Symptoms of marked hyperglycemia include polyuria, polydipsia, weight loss, sometimes with polyphagia and blurred vision. Impairment of growth and susceptibility to certain infections may also accompany chronic hyperglycemia. Acute, life-threatening consequences of uncontrolled diabetes are hyperglycemia with ketoacidosis or the nonketotic hyperosmolar syndrome. Long-term complications of diabetes include retinopathy with potential loss of vision; nephropathy leading to renal failure; peripheral neuropathy with risk of foot ulcers, amputations, and Charcot joints; and autonomic neuropathy causing gastrointestinal, genitourinary, and cardiovascular symptoms and sexual dysfunction. Patients with diabetes have an increased incidence of atherosclerotic cardiovascular, peripheral arterial and cerebrovascular disease. Hypertension and abnormalities of lipoprotein metabolism are often found in people with diabetes. Methodology Study area: Nizamabad District is located in the north-western region of Telangana state of India. The geographical limit of the district extends to 7,956 Sq. Kms and is spread over to International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 50 923 villages of 36 mandals. The district lies between 18°5’and 19° of the Northern latitudes and 77° 40’ and 78° 37’ of the Eastern longitudes. Nizamabad district is bounded by Adilabad district on the north, Karimnagar district on the east, Medak district on the south, and Nanded district of Maharashtra State and Bidar district of Karnataka State on the west. The authors have conducted an extensive field survey in the forest areas and interior villages adjoining forest areas in the district to collect the information about medicinal plants and their products used by the tribes to treat diabetes. Simultaneously actual applications of plant parts to treat diabetes were observe during the field survey. All the species have been identified and documented as per the taxonomical procedures. In course of time collected data is tabulated with botanical name, family name, local name and parts used for medicinal values. Results and discussion The paper presents a brief account of the uses of various medicinal plants and their parts in treatment of diabetes by the people of Nizamabad district, Telangana state, India. The study provides the information on 27 medicinal plant species belonging to 20 families. The plant parts used for medical preparation were bark, roots, rhizome, seeds and leaves. In some cases the whole plant was utilized. Present investigation indicates that Nizamabad district has magnificent ethno-medicinal biodiversity. Still many plants and active compounds obtained from plants have not been well characterized. More investigations must be carried out to evaluate the exact mechanism of action of medicinal plants with antidiabetic and insulin mimetic activity. Table 1 List of Plants having Anti-Diabetic Properties S. No. 1 2 Botanical Name Acacia arabica Alangium salvifolium Family Name Leguminosae Cornaceae Parts used Bark, Seeds Bark Powder, Root Liliaceae Local Name Babul, Tumma Sage leaved, Oodugu Garlic, Velluli Kalabanda Custurd Apple, Seetaphal Satavari 3 4 5 Allium sativum Aloe vera Annona squamosa Liliaceae Liliaceae Annonaceae 6 Asparagus racemosus 7 Azadirachta indica Miliaceae Neem Bark, Leaves, Seeds, Oil 8 Borassus flabellifer Arecaceae Toddy palm 9 10 Brassica nigra Butea monosperma Cruciferae Fabaceae 11 12 13 14 Cassia auriculata Catharanthus roseus Coccinia grandis Ficus bengalensis Caesalpiniaceae Apocynaceae Cucurbitaceae Moraceae Musturd, Aavaalu Flame of the forest, Moduga Tangedu Billaganneru Dondakaya Banyan,Marri 15 16 Ficus racemosa Gymnema sylvestre Moraceae Asclepiadaceae Medi Podapatri Roots, Leaves, Flowering stocks Leaves, Seeds Bark, Leaves, Flowers, Seed Gum Flower buds, Flowers Whole plant Roots, Leaves, Fruits Latex, Bark, Fruits, Leaves, Roots, Buds Bark, Fruits, Latex, Root Leaves 17 18 19 20 21 Hibiscus rosa sinensis Mimosa pudica Momordica charanita Nymphaea nouchali Pongamia pinnata Malvaceae Mimosaceae Cucurbitaceae Nymphaeaceae Fabaceae 22 23 Psidium guajava Ricinus communis Myrtaceae Euphorbiaceae Mandara Touch me not Bitter gourd Indian water lilly Indian Beech,Kanuga Jaama Castor oil, Amudam 24 Saraca asoca Caesalpiniaceae Ashoka 25 26 27 Semecarpus anacardium Syzygium cumini Tribulus terrestris Anacardiaceae Myrtaceae Zygophyllaceae Marking nut, Jeedi Black plum,Neredu Puncturevine,Palleru Bulbs Leaf juice, Roots Root, Leaves, Seeds Tuberous Root Leaves, Flowers Root, Leaves, Seeds Whole plant Leaves, Flowers, Seeds Root, Bark, seeds, Leaves, Flowers Leaves, Root, Bark Root, Leaves, Flowers, Seeds Bark, Leaves, Flowers, Seeds Fruits, Gum-resin Bark, Leaves, Fruits Root, Fruits, Leaves International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 51 Medicinal values of Euphorbiaceae Family in Nizamabad District, Telangana State S. Shireesha, Dr. A.A. Haleem Khan Department of Botany, Telangana University, Dichpally.Nizamabad.(TS) bunnishireesha@gmail.com Abstract Medicinal uses of the family Euphorbiaceae growing throughout the Nizamabad region, were studied during September 2015 to January 2016. A total of 7 species belonging to the family Euphorbiaceae were collected and identified. Out of the total number of species the identified species are Acalypha indica L., Euphorbia hirta L,Phyllanthus fraternus Webster, Jatropha gossypifolia L., Ricinus communis L., Emblica officinalis, Euphorbia geniculata Orteg. were common and Acalypha indica L is found to have rich medicinal values in the study area. The findings of this study could be used to enhance the base medicine for the betterment of rural or indigenous livelihoods. Introduction Euphorbiaceae, the spurge family, is a large family of flowering plants with 300 genera and around 7,500 species. A number of plants of the spurge family are of considerable economic and medicinal importance. The prominent areas of treatment include Asthma, Cough, Respiratory problems, Diarrhoea, Tooth ache, Jaundice, Skin diseases etc, Acalypha Indica plants are expectorant, diuretic, emetic and Laxative, for these reasons it is used in curing bronchitis, pneumonia, asthma and pulmonary tuberculosis. Study Site: Nizamabad district is situated in the northern part of the Telangana state. It lies between 18-5' and 19' of the northern latitudes, 77-40' and 78-37' of the eastern longitudes. Based on standard literature survey and related research the family Euphorbiaceae growing in Nizamabad region: S. No 1 Botanical Name Euphorbia hirta L Local Name Reddyvarinanubalu Diseases wounds and mouth ulcers Methods to Use Crushed leaf are applied. (Naikpoda) 2 Emblica officinalis Usiri anti allergic Fruits and jaggary taken in same proportion crushed and mixed taken as anti allergic. (Lambada) Fruits taken as digestive. (Lambada, Naikpod, Yarukala) 3 Jatropha curcas L Adavi amudam teeth problem *fruit powder is applied (Lambada) 4 Acalypha indica L Kuppinta, Kuppi jaundice,Scabies,itch,ring worm 5 Ricinus communis L. Amudamu Constipation 6 Euphorbia geniculata Orteg. (Sarnik’ ‘Gamalkota’ Phyllanthus fraternus Webstar. (Sarnik’ ‘Gamalkota’ Constipation Acalypa indica and Momordica charantia whole plants taken in 1:1 ratio & extract is given for 10 days Doses of 5 and 3 table spoons for adult and children respectively. (Lambada) The leaf juice can be applied externally (Lambada) Ricinus oil, honey and Zingiber officinale rhizome powder taken in to equal proportion and crushed to prepare a liquid solution. One teaspoonful taken in treating constipation. (Lambada) Eating leaf curry.(Yarukala) ‘Nala usiri’ reduce heat , laxative jaundice and leucorrhea 7 Leaf with fruits Cuminum cyminum (jeera) and sugar cubes reduce crushed with water and taken 30-40 ml. (Lambada) Whole plant eaten directly. (Lambada) Whole plant crushed and tablets are prepared, taken twice a day for 10 days. (Lambada) Cultivation and Conservation of Medicinal Plants and Women Empowerment M.Saraswathi, Asst. Professor, Dept. of Economics, G.D.C.(M), Nirmal, Adilabad. (Ph.D. Scholar, Telangana University) Abstract The Indian System of Medians, viz Ayurveda, Siddha, Unani and Homeopathic system predominantly use plant baaed raw materials m moat of their preparations and formulations. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 52 Modem pharmacopoeia also contain at least 25% drugs derived from plants and many other which are synthetic analogues bum on prototype compounds isolated from plants. India is a country of diverse cultures and rituals. Women have played a vital role in maintaining these virtues. Plants are an integral part of these cultures as they are variously utilised in the rituals. Women take an utmost care for maintaining plant species for this purpose. Agriculture is another sector which is largely dependent on women. Women play a significant and crucial role in rural economy. 90% of agricultural activities are organizing by the women in the State, like, agriculture allied fields including the main cropproduction, livestock management, post harvest operations and proccessing activities etc. But the women farmers remained as invisible workers. The socio-economic status of women in Indian agriculture is a matter of widspread concern. Low production costs and high yielding are encouraging women farmers to go for the cultivation of Medicinal and Aromatic Plants. The recent policy of liberalisation and globalisation has opened up new avenues for agriculture modernisation. Objectives of the study: 1. To study the importance of medicinal plants in the agriculture. 2. To examine the role of women in cultivation and conservation of medicinal plants in Indian agricilture. 3. To know the need and importance of conservation of medicinal plants in resource management and in employment generation. 4. To analyse the role of agriculture diversification. Hypothesis: Conservation and cultivation of medicinal plants will ensure the empowerment of women. Methodology: The methodology used in this paper with the help of the data from secondary sources. Introduction In India,the collection and processing of medicinal plants and plant products contributes a major part each year to the national economy,as a source of both full and part time employment. Micro-studies suggest that alarge numbers of those employed are women. Women have been custodian of plant genetic diversity from time immemorial. They have adequate knowledge regarding importance of plants as food, medicine, clothing and raw materials for various house-hold purposes. Plants are the backbones of religious events and rituals. Obviously these aspects of life are dependent on women. Since time immemorial, role of women has been acclaimed by society as gatherer and conservator of plant species. Medicinal plants occupied an important position in the socio-cultural, spiritual and medicinal arena of rural people of India. Their sustainable management and harvesting can conserve biodiversity, sustain human and environmental health, generate employment and enhance export earnings Therefore, a Task Force was set up, by the Planning Commission for Conservation and Sustainable use of Medicinal Plants. The World Health Organisation (WHO) estimated that 80% of the population of developing countries rely on traditional medicine mostly plant drugs, for their primary health care needs Medicinal plants being natural, non-narcotic, having no side elect of ay a range of safe, cost effective, preventive and curative therapies which could be useful in achieving the sustainable and equitable development of medicinal plants sector provide "Health for All", boost exports, and will improve livelihood of the people and green the country for the present and the generation to come. Medicinal plants sector has traditionally occupied an important position in the socio-cultural, spiritual and medicinal arena of rural and tribal lives of India. The global thrust areas for drugs from medicinal plants include disease conditions, whose incidence is increasing and where the modern drugs are either unavailable or unsatisfactory. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 53 The introduction of high-yielding varieties along with canal irrigation facilities and the lower productivity of the traditional land races are the main causes of geneticerosion of the traditional cultivars. Of the 514 000 workers registered in the district, nearly 336 000are cultivators and agricultural labourers. In the process of agricultural evolution, women haveplayed a distinct role in planting, weeding and in post-harvest operations such as harvesting,threshing, seed selection and storage. Women Empowerment and Employment creation through cultivation of Medicinal Plants Women are traditional caretakers of crop genetic diversity in agriculture. Their knowledge of the growing conditions and nutritional characteristics of various species gives them a vital fund of experience in seed selection and plant breeding. In a wider context, there to a growing demand for plant based medicines, heath products,pharmaceuticals, food supplements, cosmetics, etc,. in the national and international markets. Conservation and sustainable use of medicinal plants are issues on which immediate focus is required in the context of conserving biodiversity and promoting and maintaining the health of local communities, besides generating productive employment for the women with the objective of poverty alleviation in tribal and rural areas. At present, 90% collection of medicinal plants is from the wild, generating about 40 million mandays employment (part and full) and since 70% of plants collections involve destructive harvesting many plants are endangered or vulnerable or threatened. Currently medicinal plants are collected without paying attention to the stage of maturity. They are stored haphazardly for long period of time under unsuitable conditions. This results in deterioration in quality. Such materials are not acceptable to importers and standard manufacturing drug units. It is expected to formalize and organize medicinal plants marketing and trade, coordinate efforts of all the stakeholders of the sector and ensure health for all by improving the awakening andavailability of herbal products, besides generating productive employment to 10 million tribals and women on a regular basis. The "Medicinal Plant Board" will need a financial assistance of Rs.50 crores over a period of three years. About 90% of medicinal plants used by the industries are collected from the wild. While over 800 species are used in production by industry, less than 20 species of plants are under commercial cultivation. Over 70% of the plant collections involve destructive harvesting because of the use of parts like roots, bark, wood, stem and the whole plant in case of herbs. This poses a definite threat to the genetic stocks and to the diversity ofmedicinal plants if biodiversity is not sustainably used. Conservation and Cultivation of Medicinal Plants Medicinal plants are potential renewable natural resources. Therefore, the conservation and sustainable utilisation of medicinal plants must necessarily involve a long term, integrated, scientifically oriented action programme. This should involve the pertinent aspects of protection,preservation, maintenance, exploitation, conservation and sustainable utilization. A holistic andsystematic approach envisaging interaction between social, economic and ecological systems will be a more desirable one. The most widely accepted scientific technologies of biodiversity conservation are the in-situ and ex-situ methods. It will be necessary, based on an understanding that where medicinal plants are currently distributed, to develop novel programmes for their in-situ conservation and to designate specific genetic reserves, giving the viability of medicinal plants for generating income as well as rehabilitating degraded lands. Due to their position as the major stewards of the resource base women and tribal groups, Given the higher cost of cultivated material, cultivation is often done under contract. In the International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 54 majority of cases, companies would cultivate only those plant species which they use in large quantity or in the production of derivatives and isolates, for which standardisation is essential and quality is critical. More recently growers have set up cooperatives or collaborative ventures in an attempt to improve their negotiating power and achieve higher price. Some of the constraints associated with the processing of medicinal plants which may result in reducing their competitiveness in global markets. Systematic cultivation of many medicinal plants needs specific cultural practices and agronomical requirements. These are species-specific and are dependent on soil, water and climatic conditions. Hence research and development work has to be done to formulate good agricultural practices which will include appropriate selection and identification, propagation methods, cultivation techniques, harvesting, stepwise quality control of raw material upto processing stage, post-harvest treatment, storage and safety. These aspects have to be incorporated into protocols for the cultivation of medicinal plants; There is an immediate need to consolidate and finally link the existing herbal gardens and gene banks as well as reference specimens in herbaria to ensure that the 540 species of importance in the major classical systems, as well as those supplied to the international market, are protected in ex-situ reserves. Botanical gardens can play a key role in ex-situ conservation of plants, especially those facing imminent threat of extinction. Field gene bank of medicinal plants: The concept of establishing field gene banks of plants provides ample options for long term preservation of the genetic variability (inter-specific) of species. Involvement of competent local NGOs like Self Help Groups for organising the community and helping in implementation of programme. Building and strengthening community institutions for long term management of the sites and a management plan to be formulated for sustainable harvesting and development of medicinal plants. Organic farming is another practice that is gaining wide acceptance as world demand particularly in developed countries for organically grown crops is rapidly on the increase. Farmers have to be trained in all aspects of organic farming of medicinal plants including obtaining certification from associations that do the monitoring starting from cultivation to final harvesting. Organic farming which is labour-intensive gives the developing countries the comparative advantage to be competitive. Forest areas with biodiversity sites traditionally valued for medicinal plant diversity having a contiguous area of about 5000 hectares should be identified for creating Medicinal Plant Development Area (MPDA). The boundaries of MPDA may correspond to the boundary of reserve and protected forests. Detailed botanical studies to be carried out and stock map to be prepared for this area. Establishing a system of scientific and sustainable harvesting, drying and grading of species. Creation of market links for sale of produce at the outset of the programme. Building and strengthening forestry institutions for sustainable management of MPDA sites. Availing financial assistance from Government of India and other agencies/donors. Conclusion Plants are an integral part of these cultures as they are variously utilised in the rituals. Women take an utmost care for maintaining plant species for this purpose. Agriculture is another sector which is largely dependent on women. They collect; gather and conserve plant varieties and thus conserve the biodiversity. A large number of women from unorganised sectors are contributing significantly in the conservation of the medicinal plants Women’s contribution in resource management has not been widely used and the lack International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 55 of documentation on such fund of knowledge bars access to it and has resulted in inequality in sharing the benefits. The understanding and appreciation of gender roles in the conservation and enhancement of genetic resources guarantee gender justice in sharing the benefits. Health Benefits of Withania somnifera L. R. Rajakala,Dr. A.A. Haleem Khan Department of Botany, Telangana University, Dichpally.Nizamabad. (TS) Withania somnifera belonging to family solanaceae. It is commonly known as Ashwagandha which is one of the most powerful herbs in ayurvedic healing, has been used since ancient times for a wide variety of conditions. In sanskrit Ashwagandha means "the smell of a horse", indicating that the herb gives the vigor and strength of a horse. Its roots, seeds and leaves are used in Ayurvedic and Unani medicines. Ashwagandha, the magical herb is considered to be a nature's gift to mankind. It is very useful to treat people for their day to day problems such as stress, anxiety, exhaustion, lack of sleep etc. It has an abundance of antioxidants, iron and amino acids. It's no surprise that Ashwagandha is one of the best medicinal herbs. Ashwagandha is the 'Ginseng' of ayurvedic medicine, the traditional medicine of india and is considered an 'adaptogen' a term used to describe herbs that improve physical energy and athletic ability, increase immunity to colds and infections and increase sexual capacity and fertility. Health benefits 1. It is known for anti-inflammatory, anti-tumor, anti-stress, antioxidants, mind-boosting, immune-enhancing and rejuvenating properties. 2. Root is used in treatment of rheumatic pain, inflammation of joints, nervous disorders and epilepsy. 3. Dried roots are used as tonic for hiccup, cold, cough, female disorders and ulcers etc. 4. Leaves are applied for inflammation and swellings and its juice is useful in conjunctivitis. 5. Its extracts are used in preparation of herbal tea, powder, tablet and syrups. 6. Helps to reduce blood sugar and cholesterol levels. 7. The berries and leaves are applied externally to tumors, tubercular glands, carbuncles, and ulcers. 8. Restore male libido, cure impotence and increase male fertility. 9. The dried leaves paste is used in the treatment of burns and wounds, sunscreen upon women's faces. 10. Remedy for diabetes in Ayurvedic medicine. 11. It is effective in controlling bacterial infections in humans. 12. Its excellent protective effects on the nerves system and a promising alternative treatment for a variety of degenerative diseases such as Alzheimer’s and Parkinson’s Role of Traditional Medicine in Primary Health Care and TMK in Developing Countries Sunil Kumar A Department of Zoology, Girraj Government College (A), Nizamabad There has been a recent global upsurge in the use of traditional medicine and complementary and alternative medicine in developing and developed countries alike (Zhang, Sahai). Zhang highlights particular difficulties in protecting traditional medicinal knowledge using classic instruments for the protection of intellectual property rights (IPR). The papers by Le Quy (Viet Nam) and Dakuyo (Burkina Faso) describe the successful ways in which those countries’ Governments have promoted traditional medicine and facilitated interaction between it and western/allopathic medicine. Mhame (Tanzania), on the other hand, reports that his Government has taken a less proactive role and that consequently the sector is not as International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 56 fully developed. Shenton argues for a more effective integration of traditional healers in HIV prevention and treatment programmes in Africa. Many products based on traditional knowledge (TK) are important sources of income, food and health care for large parts of the populations of a number of developing countries. Traditional medicine plays an important role in health care in both developed and developing countries. In fact, due to their availability and affordability, the traditional medicines and therapy systems of the developing countries provide health care to the vast majority of these countries’ residents. Consequently, there is an urgent need to protect the intellectual property of traditional medicine systems. The available instruments – the patenting system and the arrangements for guarding trade secrets – are inadequate for this task, and new arrangements need to be formulated. This paper poses some key questions that need to be addressed in order to develop an acceptable solution to the problems of (i) protecting intellectual property rights (IPR), and (ii) sharing the economic benefits derived from the use of traditional medical knowledge (TMK). In designing protection mechanisms for TMK, three important issues need to be addressed: i) How should the benefits derived from the use of traditional medicine be shared? ii) How can the intellectual property rights (IPR) of the holders of TMK and scientific researchers be protected when the TMK of the former is used by the latter to create modern drugs? (In most cases knowledge of traditional medicine originates in developing countries and is appropriated, adapted, utilized and patented by scientists and industry from developed countries, with little or no compensation to the custodians of this knowledge and without their prior informed consent. In recent years, the protection of TK, the innovations and practices of indigenous and traditional medicine and the equitable sharing of benefits have received increasing attention, and they are being discussed in many international forums). iii)How can we stop the loss of biodiversity caused by the widespread use of traditional medicine and the rapidly expanding international market for herbal products? The production of herbal pharmaceuticals requires large quantities of medicinal plants, which has resulted in over collection of many plants and has made them endangered species. For example, a particular species of African potato that in 1997 was found to combat AIDS disappeared completely from its native land, the Democratic Republic of the Congo, within two years of this finding. The twentieth century witnessed a revolution in human health care. The dramatic decline in mortality, the increase in life expectancy and the eradication of smallpox are highlights of this success. Scientific innovation, leading to the development of new medicines, has played a major role in this achievement. However, despite these successes, it is estimated that over one-third of the world’s population lacks regular access to affordable essential drugs. In other words, modern medicine is unlikely to be a realistic treatment option for a substantial proportion of the world’s population. In contrast, traditional medicine is widely available even in remote areas. Due to its local availability and low cost, it is affordable by the vast majority of people living in developing countries. In India, for example, according to the Government, 70 per cent of the population uses traditional Indian medicine. In Africa the resolution on “Promoting the Role of Traditional Medicine in Health Systems: A Strategy for the African Region”, adopted by the fiftieth meeting of the World Health Organization’s (WHO’s) Regional Committee for Africa in August 2000, states that the African Member States are aware that about 80 per cent of the region’s population depends on traditional medicine for its health care needs. In the last decade, there has been a global surge in the use of complementary and alternative medicine in both developed and developing countries. According to various government and nongovernment reports from the countries in question, the percentages of the respective populations having used complementary and alternative medicine at least once are as follows: International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 57 the– Germany – 90 per cent, France – 49 per cent, Canada – 70 per cent, Australia – 48 per cent, the United States – 42 per cent, and Belgium – 31 per cent. Various reasons have been proposed for this increase, including the affordability of the treatments as well as changing needs and beliefs. The most widely used traditional medicine and complementary and alternative therapies are herbal medicines and acupuncture. Today traditional medicine and complementary and alternative medicine play an increasingly important role in the reform of the health sector of many countries. In 2000 the Secretariat of the Convention on Biological Diversity reported that the world market for herbal medicines, including herbal products and raw materials. Herbal medicines are crude plant materials, such as leaves, flowers, fruits, seeds, stems, wood, bark, roots, rhizomes or other plant parts that may be used whole or in fragmented or powdered form. It is, therefore, often not possible to seek existing patent law protection for herbal medicines by claiming the discovery of new chemical entities or development of an inventive step. Herbal products are powdered herbal materials, extracts, tinctures, or fatty oils of herbal materials prepared by steeping or heating herbal materials in alcohol and/or honey, or in other liquids. The production process is usually simple and does not involve any sophisticated know-how or invention novel enough to secure protection under existing patent laws. Zhang Traditional Medicine and Its Knowledge Except for pharmaceutical companies and industries, holders of TMK, such as research institutes and practitioners, often do not have the financial and human resources needed to obtain protection through trademarks. It is extremely difficult, if not impossible; to keep such knowledge secret because disclosure of the composition of a product is a prerequisite for registration of herbal medicines before the product can be sold as a drug. In most countries, it is very expensive to acquire, exercise, and enforce patent rights, particularly if international protection is required. For traditional practitioners and research institutions, particularly in poorer countries, the cost is prohibitive. Traditional uses and conservation of medicinal plants in Nizamabad district E. Kiran, B. Vidya Vardhini* Department of Botany, Telangana University, Dichpally, Nizamabad -503322, India Introduction: The villagers retain a strong attachment to their traditional values/beliefs.The importance of medicinal plants in traditional healthcare practices, providing clues to new areas of research and in biodiversity conservation is now well recognized. However, information on the uses for plants for medicine is lacking from many interior areas of Nizamabad. Right from its beginning, the documentation of traditional knowledge especially on the medicinal uses of plants, has provided many important drugs of modern day. Even today this area holds much more hidden treasure as almost 80% of the human population in developing countries is dependent on plant resources for healthcare. Some medicinal plant parts uses of traditionally. S.No 1 2 Botanical name/common name Achyranthus aspera L.(uttareni) Ammannia baccifera L. Disease leucorrhea wounds and cuts tooth ache Snake bite& dog bite Fever Skin deseases Traditional Uses Root extract with goat milk is given once on alternate day for 10 -15 days. Crushed leaves are applied. Roots is used as tooth brush The seeds are used anti-dote for poison of snake and dog bite Leaf juice with honey is given in empty stomach to cure typhoid. Leaf paste with common salt is applied on the affected areas to cure skin diseases such as scabies, ringworm, skin itching, etc International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 3 4 Beta vulgaris L. (Ullam gadda) Cassia auriculata L. (Tangadu) 5 6 Cissus quadrangularis L. (nalleru) Cardiospermum helicacabum 7 Celusia agentea L.(plumed cockscomb) 9 10 Gymnemasylvestre (Podapatri) Tephrosia purpurea L.(Vampali) 58 kidney stone diabetes reduces body heat Piles Rheumatism, stiffness. Diabetes, jaundice conjunctivitis liver disease, paralysis, tooth ache Daily two glass of rhizome juice for seven days Gum and dried leaves and flowers are given one teaspoon. Leaf decoction once a day. 1gm of powdered stem mixed with sugar or ghee taken for 2 to 3 weeks. Plant leaves used controlled fever, stiffness of limbs. In Indian folk medicine, used for diabetes. Seeds traditionally used for treatment of jaundice, gonorrhea, wounds and fever. Leaf taken orally. The whole plant powder with curd taken 20-30 ml orally. Root is used as tooth brush. Conservation: The preservation and careful management of the environment and of natural resources. 1.The best means of conservation is to ensure that the populations of species of plants and animals continue to grow and evolve in the wild - in their natural habitats .observe populations of medicinal plant species in natural habitats 2.The Parks should prepare a policy at national level on the conservation and utilization of medicinal plants in protected areas. 3. Public education about medicinal plants in protected areas To conserve populations of medicinal plant species ex situ: 1.Ideally all medicinal plant species should be conserved as evolving populations in nature. However, these species should also be conserved ex situ as well. 2.The primary purpose of this is as an insurance policy. But it also has the advantage that it is usually easier to supply plant material for propagation, for re-introduction, for agronomic improvement, for research and for education purposes from ex situ collections than from in situ reserves. 3.The ethnobotanical information serves as a base for new compounds with active principles for phytochemical, pharmacognostical, pharmacological and clinical research. The survival of native aquatic species is threatened and hence attention on the aquatic resources especially those having economic value are important. 4. Wetlands not only provide useful resources but ecologically very important. 5. Students, ONG’s, private organizations, Lectures and formal courses, Educational Campaigns all are protected areas. Exotic Floristic Diversity of the Nizamabad District, Telangana State, India Vijigiri Dinesh Department of Botany, Shri Renukadevi Arts, Commerce and Science College, Mahur, Nanded, Maharashtra, India. Email: vijigiri.dinesh@gmail.com A study was conducted to analyze the exotic floristic wealth of Nizamabad district of Telangana State and Nizamabad district is one of the 10 districts of Telangana state. It lies between 18-5' and 19' of the northern latitudes, 77-40' and 78-37' of the eastern longitudes. The district is bounded on the North by Adilabad district, East Bay Karimnagar District, South by Medak district and West by Bidar District of Karnataka and Nanded district of Maharashtra. The geographical area is 7956 Sq. Km’s i.e. 19, 80,586 acres spread over 923 villages in 36 mandals. Major rivers, such as, Godavari and Manjeera crosses Nizamabad district with some other streams Kalyani, Kaulas, Peddavagu also exist in the district. Flora refers to the botanical composition of a place where names of different species are taken into account. Flora is widely categorized into two type’s i.e. native flora and exotic flora. Native floras are those which exist from pre-historic times in the country while exotic International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 59 flora is those which are of foreign origin. Nearly 40% of the plant species found in India are exotic. A total of 144 exotic plant species distributed among 118 genera belonging to 54 families were collected. The flora of Tropical American origin dominates the exotic floristic composition of the Nizamabad district. Key Words: Exotic Flora, Diversity, Nizamabad, Telangana State. Study of Various Medicinal Benefits of Spirulina on Human beings M. Srinivasa Rao1 and P. Latha2 1 Dept. of Microbiology Govt. Degree College, Armoor, Nizamabad, Telangana State 2 Dept. of Botany, Govt. Degree College, Armoor, Nizamabad, Telangana State Single-cell protein (SCP) refers to edible unicellular microorganisms. The biomass or protein extract from pure or mixed cultures of algae, yeasts, fungi or bacteria may be used as an ingredient or a substitute for protein-rich foods, and is suitable for human consumption or as animal feeds. The algae spirulina has been considered for use as a supplementary protein. It is a blue green algae having strong antioxidant activity and provokes a free radical scavenging enzyme system. Medicinal uses of spirulina is strengthen and improve immune system, phycocyanins build blood cell, increase antiviral activity, Exhibits, anticancer activity.Studies showed that a spirulina consumption of 4 weeks reduced serum Cholesterol level in human beings by 4.5% and significantly reduced body weight by 1.4±0.4kg after 4 weeks. It is concluded that the use of spirulina should be encouraged in patients suffering from malnutrition, immuno-suppression, hepatic and neural compromise, etc. The reduction of Cholesterol is partly due to high content of gamma linolenic acid in cyanobacteria. This paper studies the medicinal benefits of Spirulina for obtaining improvement of health on human beings. Keywords:Antioxidant, Health, Human, Single cell protein, Spirulina. A study of locally available medicinal plants of Ananthagiri hills forest area and their role in folklore medicine. N.C. Sowjanya, Dept. of Botany, Government City College (A), Hyderabad Medicinal plants, since times immemorial, have been used as a source of medicine. The widespread use of herbal remedies and healthcare preparations, are described in ancient texts such as the Vedas and the Bible. The use of traditional medicine and medicinal plants in most developing countries, for the maintenance of good health, has been widely observed. Furthermore, an increasing reliance on the use of medicinal plants in the industrialised societies has been traced to the extraction and development of several drugs and chemotherapeutics from these plants as well as from traditionally used rural herbal remedies. Moreover herbal remedies have become more popular in the treatment of minor ailments, and also on account of the increasing costs of personal health maintenance. Therefore, the present study emphasizes on identifying the locally available medicinal plants in the Ananthagiri forest area and also to document their role in folklore medicine. Micropropagation, molecular profiling and RP-HPLC determination of Woodfordin C & Woodfordin I across various regeneration stages of Woodfordia fruticosa (L.)Kurz Mahender Aileni Dept. of Biotechnology, Telangana University, Dichpally, Nizamabad- 503 322 Woodfordia fruticosa (L.)Kurz, (family – Lythraceae.) a woody shurb isproved to be a rich storehouse of pharmaceutically active compounds used in treating various diseases. The compound woodfordin C (an inhibitor of DNA topoisomerase II) exhibits antitumor activity.Woodfordin I, shown to induce apoptosis in human chronic myelogenous leukemia (CML) K562 cells. High demands from pharmaceutical industries lead the indiscriminate International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 60 harvesting from the wild which created the pressure on natural populations. Micropropagation system was developed using different explants for both commercialization and conservation of W. fruticosa. Murashige and Skoog’s (MS) medium with different concentrations and combinations of plant growth regulators (PGRs) viz. 6-benzylaminopurine (BAP), kinetin (KN), indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy acetic acid (2,4-D) were used during various in vitro regeneration stages. MS medium supplemented with BAP (3.0 mg/l), maximum rate of shoot multiplication (90%) exhibited highest regeneration efficiency with 35 ± 1. 65 multiple shoots/ explants. The antioxidants and other additives significantly altered the rate of shoot multiplication, number of shoots as well as survival rate of shoots. The plantlets rooted on ½MS + IBA 1.5 mg/l, showed 80% establishment in soil without any morphological variation. The genetic integrity of the regenerated plants was further confirmed using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analysis. A total of 3871 distinct bands were produced by 12 RAPD and 17 ISSR primers in mother and 20 randomly selected micropropagated clones wherein 3858 (99.66%) bands were monomorphic and rest of 13 (0.34%) were polymorphic. The high level of monomorphism confirms the genetic uniformity of the micropropagated clones. Additionally, reverse phasehigh performance liquid chromatographic (RP-HPLC) analysis at various in vitro regeneration stages showed uniformity in terms of presence of Woodfordin C & Woodfordin I however, the amount of Woodfordin C & Woodfordin I varied across regeneration stages. Phytochemical and molecular fingerprinting of micropropagated clones revealed that the in vitro regeneration protocol developed could be successfully used for industrial-scale propagation of W. fruticosawith enhanced production of Woodfordin C & Woodfordin I. Keywords: Antitumor, Antileukemia , ISSR , RP-HPLC, Mature node, Micropropagation, RAPD ,Woodfordin C & Woodfordin I Isolation and identification of some pathogenic fungi from different infected vegetables Digambar S.Pawar and Sahera Nasreen Dept.of Botany, Government Institute of Science and Research Center, Nipat Niranjan nagar, Caves road, Aurangabad-431004 Corresponding author – dspawarbotany@gmail.com ABSTRACT The present work deals with the isolation and identification of pathogenic fungi. The fungal isolates were Colletotrichum capsici, Phytopthora infestans, Fusarium oxysporum, Fusarium moniliforme, Taphrina maculans, Alternaria alternata and Alternaria solani which were isolated from vegetable and were identified on the basis of colony morphology, microscopic examination by using selective and differential media. The morphological characteristics of these fungal elements showed various kinds of spores have been identified up to genus/species level. Keywords: Colletotrichum, Phytopthora, Fusarium, Taphrina, Alternaria INTRODUCTION Plants are infected by different kinds of microbial pathogens and the required inoculum for infection is present in the soil, water and air, in addition to plant host. In many cases, the pathogens may be carried by seeds or propagative planting materials such as tubers, corms, suckers and setts. Whatever may be the source of inoculum, the susceptible plant species or crop varieties may exhibit clear visible local symptoms in or on the tissues where infection is initiated. If the pathogen is able to find favourable conditions for further development, systemic symptoms are induced in tissues or organs far away from the point of pathogen entry into the plant. When the symptom of infection is not expressed externally, it is termed latent infection. Some fungal pathogens infecting unripe fruits do not induce any visible symptom, as they remain dormant Detection of microbial pathogens refers to the process of International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 61 establishing the consistent presence of a particular target organism(s) within the plant or in its environments, irrespective of the development of visible symptoms in the plant suspected to be infected by the pathogen(s) in question. Diagnosis, on the other hand, relates to the identification of the nature and cause of the disease problem under investigation. MATERIALS AND METHODS Infected plant materials were collected in sterile polythene bags from various fields from the Mantha taluka of Jalna district. Collected infected parts of Turmeric , Potato, Pumpkin , Cabbage and lady’s finger were cut into small pieces, and then rinsed 3~4 times with sterile Distilled water after treated with 70% (v/v) ethanol for 2~3 s and with 0.1% (w/v) mercuric chloride solution for 3~5 min. The infected parts of the leaves were places into moisture chamber for 2 to 3 days for the growth of fungi. After the growth of fungal mycelium then it was transferred to PDA plates and Incubated for 3-5 days for the complete growth of fungi with sporulation. All the plates were grown in triplicates for the complete isolation and purification of plant pathogenic fungi the microscopic photo plates were tabulated. Medium used for the isolation: The composition of the differential medium used for the isolation and identification. PDA media Potato Distilled water Nutrient agar - 200 g Dextrose Agar agar - 20 g - 20 g - 1000 ml. Peptone Beef extract Yeast extract Sodium chloride Agar agar Distilled water - 5.0 - 1.5 - 1.5 - 5.0 - 15.0 - 1000ml Sabouraud Dextrose agar Peptone Dextrose Agar Distilled water - 10.0 g - 40.0g - 15.0g - 1000 ml Identification of fungi: The isolated fungi were identified to the genus level and species which was possible on the basis of micro-morphological and macro-morphological characteristics using suitable media, slide cultures (obtained by inoculating micro fungi directly on a small square of agar medium) and the most updated keys for identifications. Photoplate 1:-Growth of pathogens on differential media A. alternata F. oxysporum C. capsici T. maculans F. moniliforme A. solani Photoplate 2:- Growth of pathogens on PDA medium P. infestans International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health P.infestans F.moniliforme 62 C.capsici T.maculans A. alternata A.solani F.oxysporum Table1.-Showing the Isolated Pathogen with Respective Host Sr.No. 1. 2. 3. 4. 5. 6. 7. Name of Fungal Mycelium Fusarium oxysporum Alternaria alternata Fusarium moniliforme Colletotrichum capsici Taphrina maculans Alternaria solani Phytopthora infestans Host Abelmoschus esculentusL. Abelmoschus esculentusL. Cucurbita pepo L. Curcuma longa L. Curcuma longa L. Solanum tuberosum L. Solanum tuberosum L. Month Sept. Sept Sept. Oct Oct Sept. Sept. RESULT AND DISCUSSION During the investigation period seven fungus were isolated from infected vegetables parts. Colletotrichum capsici, Phytopthora infestans, Fusarium oxysporum, Fusarium moniliforme, Taphrina maculans, Alternaria alternata and Alternaria solani were observed. It is found that maximum percentage contribution was observed for .Fusarium moniliformi and F. oxysporum. During the investigation period (Between July –October) monthly seasonal variation were also observed. Five fungal species were found in the September month due to low temperature, percentage of humidity it was quite favourable for fungal growth.and remening two Fungal species were found in Oct. Month. The results of present investigation revealed with work done which was recorded in the table 1. These include Alternaria, Aspergillus, Candida, Cephalosporium, Cladosporium, Fusarium, Geotrichum Mucor. Sharma (2010) Sharma et al (2011) al, (2006), Penicillium, Rhizopus, Rhodotolura, Saccharomyces, Torulopsisand Trichoderma. Oboh et al, (2006) reported that the fungal isolates obtained in their study were mainly Aspergillus species, while others were Trichoderma, Penicillum, Rhizopus and Rhodotorula species which were all able to utilise hydrocarbon as carbon source. Our finding coincides with the work of Elisane et al, (2008), who also isolated four strains from the contaminated soil. They were identified as Aspergillus sp. Kostadinovaa et al (2014) isolated Aspergillus andPenicillium from Antarctica. Sharma (2010) isolated same fungiat Darjeeling tea garden soil and Sharma et al (2011) reportedsome same fungi from Lachung soil.the result were compared with the study of other workers for for the fungal strains Colletotrichum capsici, Phytopthora infestans, Fusarium oxysporum, Fusarium moniliforme, Taphrina maculans, Alternaria alternata and Alternaria solani. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 63 Traditional knowledge on medicinal plants used in the treatment of mouth ulcers in rural villages of Mahabubnagar District, Telangana, India. P. Shivakumar Singh1*, D.S.R. Rajender Singh2, G.M. Vidyasagar3 1 Department of Botany, Palamuru University, Mahabubnagar-509001, Telangana, India. 2 Principal, Department of Botany, SV Govt. UG & PG College, Palem, Mahabubnagar, 3 Medicinal Plants and Microbiology Research Laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, Gulbarga –585 106, Karnataka, India. ABSTRACT The method of traditional healing has deep root in Indian culture used the plants not only for curing diseases but also during various ceremonies. Right through man history, people have relied on traditional medicine, in particular to promote and maintain good health. This addiction of man on plant ended him to obtain the knowledge on medicinal properties of plants by documenting. A total of 32 folkloric medicinal plants used in the treatment of mouth ulcers in ten villages of Mahabub nagar district, Telangana state have been documented using standard questioner. Of 31 plants species belonging to 31 genera and 22 families were found useful. The leading family Fabaceae was represented 04 species followed by Euphorbiaceae 03 species, whereas the five families i.e., Apiaceae, Lythraceae, Lamiaceae, Myrtaceae and Verbenaceae represented by 2 of each species. While the 15 families represented single species each. Among parts used 20 plants species 12 used with ingredients, others alone independent plant parts used. The present report described them in detailed along with the method of drug preparation, mode of administration, probable dosage and duration of treatment. Utmost new results on the rural practices were documented. Key words: Traditional medicinal plants, mouth ulcers, Mahabubnagar, Telangana. INTRODUCTION The skill of rural peoples in medicinal plants and their usages has very yawning involved in Indian culture. The plants are being used not only for curing diseases but also during various ceremonies. The present to man history, people have directly or indirectly depending on rural medicinal plants in scrupulous to endorse and sustain fine healthiness. This reliance of man on plant ended him to gain the information on medicinal knowledge on plants by audition methods. The medicinal usages and their wisdom of plants have giving numerous information on drugs preparation, mode of action secrets from our fore fathers. Even these days this area holds much more concealed riches as about 80% of the human population in embryonic countries is reliant on plant products for healthcare. The familiar mayhem mouth ulcers are stipulation that affects the common population at one time or another. The majority of prescription that has been used so far i.e. mouth washes and gels have the inconvenience of concentrated contact time with the exaggerated area. Oral sanitation is an vital part of health of a individual. Oral health when disused, results unmoved types of oral ailments like mouth ulcers, dental caries and periodontal diseases. Oral disorders can considerably influence the general well-being of a individual by causing considerable pain and uneasiness, thus distressing their excellence of life [1]. Telangana State is one of the 29 states of India. It was separated from the erstwhile Andhra Pradesh on 2nd June 2014. Telangana State is situated in the central stretch of the eastern seaboard of the Indian Peninsula. Telangana state has an area of 114,840 square kilometres (44,300 sq mi). The area is divided into two main regions, the Eastern Ghats and the plains. Telangana lies between 15 50' – 19 55' North latitudes and 77 14' – 78 50' East longitudes. Telangana is bordered by the states of Maharashtra to the north and north-west, Karnataka to the west, Chattisgarh to the north-east and Odisha to the east and Andhra Pradesh to the south. The state is drained by two major rivers, with about 79% of the Godavari river catchment area and about 69% of the Krishna catchment area, but most of International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 64 the land is arid. It is an extensive plateau with an average elevation of about 400 m above sea level. This plateau consists mainly of the ranges of erosion surface: (i) above 600 mt, (ii) from 300 – 450 mt and (iii) from 150 – 300 mt. The State Telangana has the monsoon type of tropical climate. On the whole State enjoys warm climate. In northern Telangana tropical rainy type of climate prevails. Hot Steppe type of climate is noticed in the southern parts of the State. In Tropical Rainy type, the mean daily 0 temperature is above 20C with an annual rainfall of 150 to 200 cms, mostly in summer and South-West monsoon. In the Hot Steppe type, the mean daily temperature is 18C and less. In the state of Telangana Maximum temperature in the summer season varies between 37C and 44C and minimum temperature in the winter season ranging between 14C and 19C. The State has a wide variety of soils and they form into three broad categories - red, black and literate. The type of forests met within Telangana, as perthe classification of Champion and Seth (1968) are Tropical moist deciduous forests, Southern dry deciduous forests, Northern mixed dry deciduous forests, Dry savannah forests and Tropical dry evergreen scrub 9 . Figure 1, Study area: Mahabubnagar District, Telangana State, India. Mahabubnagar is the largest district in Telangana in terms of area (18432.00 sq. km) covered. It is also known as Palamoor. The name was changed to Mahabubnagar in honour of Mir Mahbub Ali Khan Asaf Jah VI, the Nizam of Hyderabad (1869-1911 AD). The district was situated between 77° 15' and 79° 15'E, of the eastern longitudes and 15° 55' and 17° 20'N, of northern latitudes. Mahabubnagar is southern district of Hyderabad state under Nizam and bordered with River Krishna in the south and surrounded by the Guntur District of AP to the east, Kurnool district of AP to the south, Nalgonda and Ranga Reddy Districts to the north and Gulbarga and Raichur Districts of the state of Karnataka to the West. The district has population of 40,53,028 as per the 2011 census which accounts for 11.52% of the total population of the State with 15.34% decadal growth. The people of this district are economically backward. They can speak three languages, knowledge flows from one culture to other. The plant assortment is very rich and a excellent quantity of medicinal plants are using in the treatment of various diseases including mouth ulcers. There was no detail document on mouth ulcers contribution from the study area. Therefore, the present research concept focused on the credentials of rural comprehension on medicinal plants used in the healing of mouth ulcers. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 65 MATERIALS AND METHODS Regular field trips were undertaken to different localities of the district [study area] during the period from June 2014 to June 2015. Localities were selected in such a way that they should represent the entire district including rural areas. A list of healers belonging to different areas was prepared. Each locality was visited during special seasons and collected the in sequence concerning the seasonal medicinal plants. During the fieldwork, numerous visits were made to the herbal practitioners and efforts were ended to convince them to divulge their rural knowledge about the remedial plants. The information about the plants was systematically recorded by revenue of negotiations and interviews using standard questionnaire [2] with the informers along with the field visits during the collection period. The information was gathered like local name(s) of the plant, parts used. The information about the ingredients supplements, methods of administration, coupled medicated food was also recorded in few cases. The collected plant specimens were authentically identified with the help of floras such as, Flora of Andhra Pradesh, [3], Flora of British India [4], Flora of Telangana [5]and the voucher numbers were given and herbarium specimens were prepared, submitted. RESULTS AND DISCUSSION The present rural report on medicinal plants revealing a total of 31 rural medicinal plants used against mouth ulcers in rural area of Mahabub nagar district. Telangana state have been documented using standard questioner. Of 31 plants species belonging to 31 genera and 22 families were found useful. The leading families Fabaceae and Euphorbiaceae with each of 03 species, whereas the five families i.e., Apiaceae, Lythraceae, Lamiaceae, Myrtaceae and Verbenaceae represented by 2 of each species. While the 15 families revealed single species each. Among parts used 20 plants species were used without ingredients, while 11 used with ingredients. The current report described them in meticulous along with the vernacular name, part used, plant botanical name, family. Maximum new findings on the ethnic practices were recognized [Table 1]. The data was compared with the available recent literature and found that many of the usages programmed are not recorded previously [6, 7]. Earlier there were no reports on mouth ulcers from the present study area. This is first and unique report. Incidence in circulation sources, fraction in division of growth forms, allotment of medicinal plants into systemic groups, occurrence in allocation of plant parts against mouth ulcers was analyzed. Total 100 Wild (In-situ) 100 80 60 28.12 40 9 20 32 0 8 25 No. of Medicinal plants Percentage (%) Cultivated (Ex-situ) 15 46.87 Both Fig. 1: Incidence allocation of sources of medicinal plants from the study area against mouth ulcers Frequency distribution sources of medicinal plants in the study area against mouth ulcers shown in fig.1. Of 31 plants species recognized, shows that both the (Cultivated and International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 66 Wild)share the biggest fraction with 15 species (46.87%) followed by Wild (In-situ) with 09 species (28.12%) and Cultivated (Ex-situ) with 08 species (25 %). 60 Percentage (%) 50 40 40.62 30 31.25 20 25 10 8 0 Herbs 13 10 Shrubs 3.12 1 Climbers Trees 0 0 Figure: 2: Fraction allocation of expansion forms of medicinal plants recorded against mouth ulcers. The analysis of habits (Fig. 1) of plants predictable, shows that trees share the leading section with 13 species (40.62%)followed by shrubs with 09 species (31.25%), herbs with 08 species (25.00%) and climbers with 1 species (03.12%). Euphorbiaceae Fabaceae Lamiaceae Verbenaceae Apiaceae Lythreceae Myrtaceae . Fig 3: Medicinal plants circulation family wise adjacent to mouth ulcers. Frequency of occurrence 100 37.5 Total Frequency of occurrence Flower buds Leaf base Terminal buds Fruit Rhizome Latex Flowers Seeds 12 3.126.259.37 32 1 2 3 3.126.253.1215.629.37 1 2 1 5 3.12 3 1 3.12 1 Leaves Aerial parts Bark 100 80 60 40 20 0 Figure 4: Incidence allocation of plant parts used against mouth ulcers. International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Proceedings of National Seminar on Medicinal Plants For Better Human Health 67 In the present analysis 31 plants wisdom, their local name, part used, botanical name and family were authenticated. This was possible due to the fact that rural population of study area is much larger and related to utilize of plants for healthcare upholding, because of the basic density of flora is found in the study area. The present report Lawsonia inermis L. terminal buds listed, but in previously Maji Jose et al., used only fresh leaves.While Jatropha curcas L. latex reported, while in formerly the leaves used in the treatment of mouth ulcers [7]. The Rajasthanees used Jatropha species in treating habitual mouth hygiene [8]. Hebber et al., [9] reported 35 plants belonging to 26 families used to indulgence altered mouth ailments of Western Ghat of Dharwad district of Karnataka. In the previous dental [mouth] related ailments reported by Chopra RN et al., [10]. Cinnamon inhibits the growth of oral microbes [11]. Sonowal Kachari [12] reported two effective medicinal plants against mouth freshening. Vidyasagar GM et al., [13] were reported 32 anti mouth ulcer medicinal plants and their detailed usages. CONCLUSION Due to the documentation of information on rural medicinal plants against mouth ulcers would be improving the socio economic position of the people. This study gives foundation for further phytochemical and pharmacological studies. Table 1: Traditional knowledge on medicinal plants used in the treatment of mouth ulcers in rural villages of Mahabubnagar District, Telangana, India. Vernacular name Adavi gobi Allam Parts used Leaf Rhizome Botanical name Launaea procumbens(Roxb). Zingiber officinale Rosce. Family Asteraceae Zingiberaceae Avichi chettu Daanimma chettu Dante Jama Jeera Jeripothu alamu Leaves & flowers Seed Leaf Leaves Seed Latex Cassia alata L. Punica granatum L. Gymnosporia montana (Roth) Benth Psidium guajava L. Cuminum cyminum L. Argemone mexicana L. Ceasalpiniaceae Lythraceae Celastraceae Myrtaceae Apiaceae Papaveraceae Kakar kaya Kalepaku chettu karaka Kashabusha chettu Kebbera Kothimir Lavanga Dried fruit pulp Leaf Fruits Leaf Fruit Ariel Flower buds Cucurbitaceae Rutaceae Combretaceae Solanaceae Arecaceae Apiaceae Myrtaceae Mamidi Marri chettu Seed Terminal buds Momordica charantia L. Bergera koenigii L Terminalia chebula L. Solanum nigrum L. Coccus nucifera L. Coriandrum sativum L. Syzygium aromaticum (L.) Merr. & Perry Mangifera indica L. Ficus bengalensis L. Midaku Muduga Nalla thumma Napalamun Nela usiri Neredu Podina Pulichara chettu Seema chintha Seetha phala chettu Teku Tulasi Vailu chettu Vepa chettu Terminal buds Leaf Leaves Latex Dried fruit powder Leaves Leaf Young stem bark Fresh fruit Flower Leaf base Leaf Terminal buds smooth stem bark Lawsonia inermis L. Butea monosperma (Lam.) Taub. Vachellia nilotica (L.) P.J.H.Hurter & Mabb. Jatropha curcas L. Phyllanthus emblica L. Zizyphus jujuba Lam. Mentha viridis L. Phyllanthus reticulates Poir. Pithacalobium dalsi L. Annona squamosa L. Tectona grandis L. f. Ocimum sanctum L. Vitex negundo L. Azadirachta indica Lythraceae Fabaceae Fabaceae Euphorbiaceae Euphorbiaceae Rhamnaceae Lamiaceae Euphorbiaceae Fabaceae Annonaceae Verbenaceae Lamiaceae Verbenaceae Meliaceae International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in Anacardiaceae Moraceae Proceedings of National Seminar on Medicinal Plants For Better Human Health 68 About Editors Prof. B. Vidya Vardhini is a Professor and Head in the Dept. of Botany, Telangana University. She is Principal, University College of Science. She has 21 years of teaching and research experience. She completed her M.Sc. (Botany) in first division with distinction in the year 1994. She worked as a Project Fellow in the UGC Sponsored MRP from 1995 - 1998. She was awarded Ph. D. (Doctor of Philosophy) in 1998 from Osmania University, Hyderabad under the guidance of Prof. S. Seeta Ram Rao. She worked as a Research Associate in the CSIR sponsored MRP from 1999- 2002. She worked CSIR- Senior Research Associate (Scientist’s Pool Scheme) from 2002-2003 at Osmania university, Hyd. She worked as a Lecturer/Asst Professor in Botany on selection by Union Public Service Commission in Union Territory of Puducherry, from 2003 to 2008. She served as the Head, Department of Botany, Chair Person, BOS in Botany as well as Biotechnology and Director, Alumni; Director, Research and Development etc. She completed a UGC-MRP in 2007- MRP-655/05 (UGC –SERO) on brassinosteroids and saline soils of Karaikal. She published more than 56 research papers in National and International journals with good Impact Factors like Phytochemistry, Plant Growth Regulation, Indian Journal of Plant Physiology, Current Science, Asian and Australasian Journal of Plant Science and Biotechnology, Bulgarian Journal of Agricultural Sciences etc. Her total Impact Factor is 30.902, citations are 605 and h-index and i10-index are 10. She has presented around 45 papers in National and International seminars /conferences etc. She was Lead Guest Editor for Journal of Phytology, Issue 4 volume 2, 4 and 5, Year 2012, Khajure Research Foundation and Development Society, Scholar Journals, Unites Arab Emirates. She is the Reviewer for African Journal of plant Sciences, Journal of Crop Improvement, Australian Journal of Experimental Agriculture, Food Science and Technology, Plant etc. and Editorial Board Member of Agriculture, Forestry and Fisheries, USA and Journal of Applied Science and Research, USA. She is guiding 5 Research scholars from T.U and 1from JNTU. Dr. Ahmed Abdul Haleem Khan Assistant Professor, Dept. of Botany, University College, Telangana University, Dichpally,Nizamabad-503322, Telangana State E mail: aahaleemkhan@gmail.com Research Project: UGC-BSR Research Start-Up Grant project, A Study on the Biodiversity of Endophytic Fungi in Apocynaceae, Ref: Lr. No. F. 30100/2015 (BSR) (on going)-Rs. 6.00 lakhs Invited Lectures in National seminars & Extension Lectures Delivered: 13 Membership in Professional Bodies/ Societies: Indian Science Congress Association (ISCA) Membership No. L14922, Medicinal and Aromatic Plants Association of India [MAPAI] Membership No. 360 Countries visited: Malaysia, Kingdom of Saudi Arabia Co-curricular Experience: Member, Board of Studies, Department Research Committee, Mentor, Dept. of Botany, Volunteer editor/reviewer for Science Alert scientific journals, Member in Editorial Board for International Journal of Biology, Pharmacy and Allied Sciences (IJBPAS), ISSN: 2277-4998 Organized three Lecture Workshopsponsored byIndian Academy of Sciences, Bangalore, Indian National Science Academy, New Delhi, The National Academy of Sciences, Allahabad.Popular Lecture Series sponsored by Department of Biotechnology ( DBT)- CTEP Training: 12 workshops, 1 refresher course, 1 orientation programme, Publications: Book: 01, Chapters: 03, Papers: International: 10, National: 06, Conf. Proceedings: 15 Citation indices-Google Scholar: Citations: 60, h-index: 4, i10-index:2 Total Impact factor: 27.669 International Science Community Association www.isca.in , www.isca.co.in , www.isca.net.co , www.isca.net.in