A TRANSPONDER FOR HARMONIC RADAR TRACKING OF THE BLACK VINE WEEVIL IN BEHAVIORAL RESEARCH R. D. Brazee, E. S. Miller, M. E. Reding, M. G. Klein, B. Nudd, H. Zhu ABSTRACT. The black vine weevil (BVW), Otiorhynchus sulcatus (Fabricius), is a major economic insect pest for growers of ornamental nursery crops and small fruits. Development of management strategies by entomologists and growers has been hampered by a lack of behavioral information on movement of BVW within agroecosystems. Although insects can be tracked using tag-and-release methods, the BVW is active primarily at night, cannot fly, and can be difficult to relocate. Harmonic radar technology has been used in entomological research and was investigated for applicability to the BVW problem. An insect-mounted, miniature transponder was developed to facilitate location in conjunction with a commercially available harmonic radar transceiver detector. The transponder, powered by a 0.917 GHz signal from the detector, returns a 1.834 GHz signal when detected. The transponder consists of a Schottky barrier diode with an inductively loaded monopole antenna and is lightweight at about 27% of a BVW body mass. In field trials, insects were successfully released, relocated, and recovered after several days. Keywords. Antenna, Insect, Instrumentation, Nursery, Pesticide. T he black vine weevil (BVW), Otiorhynchus sulcatus (Fabricius), is a particularly troublesome pest for growers of ornamental and other nursery crops. Shetlar (1995) provides an informative overview of the problem, noting that the BVW ranges across much of North America from Maine to the Carolinas and west to Washington and Oregon. The BVW feeds on over 100 different kinds of trees, shrubs, vines, and flowers, with the preferred hosts in nurseries growing ornamentals typically being Taxus, hemlock, and rhododendron. In addition to field and landscape plantings, it commonly infests containerized perennials in greenhouse and polyhouse production settings. Adults often feed along leaf margins, producing typically crescent shaped notches, while the larvae prefer to feed on young tender roots. Adult BVW are oblong oval in shape, about 13 mm long, and while they cannot fly, they are very active walkers. With regard to control, knowledge about the behavior and movement of this insect in and around nurseries is very incomplete. This lack of knowledge causes growers to apply insecticides against BVW after the resident population Article was submitted for review in April 2004; approved for publication by the Information & Electrical Technologies Division of ASAE in February 2005. Presented at the 2004 ASAE Annual Meeting as Paper No. 041033. Mention of proprietary product or company is included for the reader’s convenience and does not imply any endorsement or preferential treatment by USDA-ARS. The authors are Ross D. Brazee, ASAE Member Engineer, Senior Research Scientist, Ethan S. Miller, Former Research Associate, Michael E. Reding, Research Entomologist, Michael G. Klein, Research Entomologist, Barry E. Nudd, Electronic Technician, and Heping Zhu, ASAE Member Engineer, Agricultural Engineer; USDA-ARS Application Technology Research Unit, Wooster, Ohio. Corresponding author: Heping Zhu, USDA-ARS Application Technology Research Unit, Ag. Eng. Bldg., OARDC, 1680 Madison Ave., Wooster, OH 44691; phone: 330-263-3871; fax: 330-263-3670; e-mail: zhu.16@osu.edu. should have been eliminated because they fear migration of the insect into nurseries from outside sources. These preventative treatments are costly in terms of time, labor, materials, and environmental risks, and may be unnecessary. Clearly, data are needed on BVW behavior to aid development of improved or alternative management strategies and more effectively time and target those control measures. Tracking of the insects is an essential part of behavioral studies. Entomological radar technology offers some interesting possibilities for adapting or developing research tools for investigating insect behavior, populations, and migrations (Beerwinkle et al., 1994; Wolf et al., 1993, 1995). It is immediately clear that radar applications in nursery insect research present different challenges than, e.g., long-range migration studies, since tracking must be done in more restricted areas with plant populations of wide variety. Since the BVW prefers to live in or near these sorts of habitats, harmonic radar appears to have potential as a tracking research tool. Unlike a conventional radar system, which depends on the target to reflect sufficient radio energy for detection, harmonic radar systems use tuned reflectors mounted on targets of interest. The reflectors are powered by an incoming radar signal from a tracking transceiver, or detector. For entomological studies, tiny transponders must be attached to insects that are either mass-reared or captured and released for study. The harmonic radar detector sends a signal at a known fundamental frequency and receives return signals from the target transponder at twice the transmitted frequency (the 2nd harmonic, as described later). The reflector circuit on the target typically consists of an antenna and a Schottky barrier diode, which effectively multiplies the received signal to produce the 2nd harmonic signal detected by the receiver. This mode of operation prevents excitationsignal backscatter from interfering with detection of the returned tracking signal from the transponder. Transactions of the ASAE Vol. 48(2): 831−838 2005 American Society of Agricultural Engineers ISSN 0001−2351 831 Mascanzoni and Wallin (1986) used harmonic radar technology for tracing released, individually marked specimens of various nocturnal species of ground-dwelling carabid beetles in a cereal field. They were able to continuously trace individual insects for periods of up to 8 days. Recapture rates for radar-tagged insects indicated no significant effect on behavior compared with other tracing methods. Of the total number of insects recaptured, 86% of the tagged and 83% of the control insects were recovered within a week. Their diode-equipped tags used antennas of 3 to 5 cm lengths, with weight for some tags of the order of 30 to 80 mg. Tracking ranges indicated from their data for these tags were about 4 to 6 m. Overnight travel distances for the various insects observed averaged 7.9 m with a range of 3.2 to 16.2 m. Other applications of harmonic radar technology include studies of bee flight and foraging patterns. Capaldi et al. (2000) used harmonic radar to demonstrate ontogeny in orientation flights of honeybees. The bees take repeated flights at increasing distances to develop homing capability, as they become successful foragers at about three weeks of age. Osborne et al. (1999) studied dynamics and spatial scale of bumblebee foraging flights. Transponders consisted of Schottky diodes with vertical dipole antennas and loading coils constructed from with 135 mm copper-plated springsteel wire, and weighed about 12 mg. Roland et al. (1996) used harmonic radar to study effects of habitat on movement of several species of flying insect. Tags were fitted to the small Apollo and alpine butterflies, to larvae and moths of the forest tent caterpillar, and to the parasitic tachinid and sarcophagid fly, both of which attack the tent caterpillar. The tags that were used consisted of a Schottky diode and center-driven dipole antenna formed from extremely fine aluminum bonding wire. The tags increased weight of the insects by about 0.1% to 0.9% and typically weighed about 0.4 mg. Using a 1.7 W continuous transmitted radar signal, they report detection ranges up to 50 m. Boiteau and Colpitts (2001) investigated the effect of electronic tag weight on flight performance of the Colorado potato beetle. They found that transponders should weigh no more than 23% to 33% of the beetle’s body weight to have minimal impact on the number and quality of upward flights. They also reported from chamber trials that beetles were incapable of upward flight beyond 11.8 N m−2 average wing loads. With the aid of harmonic radar, Svensson et al. (2001) monitored long-range, pheromone-mediated flight behavior of male turnip moths (Agrotis segetum) under natural and mating disruption conditions. Under various treatments, moths could be followed over a variety of terrains for up to 77 min, corresponding to a total track length of 7350 m and average ground speeds ranging from 0.7 to 5.4 m s−1. Tag weight was about 8 mg, amounting to less than 10% of the insect’s body weight. Generally, no great amount of detail was given on transponder properties, such as antenna impedance, efficiency, or far-field characteristics near the ground. Despite the desirability of having such information, those numbers are not always easy to obtain, considering the variety of needs for each tracking application and the difficulties associated with high-frequency measurements. Thus, the primary goals of this study were: (1) to determine the potential of harmonic 832 radar technology for tracking of the BVW, given its ground-dwelling and cover-seeking tendencies, and (2) to identify and develop the transponder-antenna combination that would be required. THE HARMONIC RADAR SYSTEM THE RADAR TRANSCEIVER The radar transceiver chosen for this research was a RECCO Type R5 detector (RECCO AB, Lidingö, Sweden) that is designed for locating avalanche victims wearing special transponders. The system transmits a 0.917 GHz fundamental excitation signal from an integral Yagi-Uda antenna at about 1.7 W, receiving a 2nd harmonic response at 1.834 GHz from any transponder within range and located in the same direction as the main forward lobe of the antenna transmission pattern. The operator receives an audible signal of transponder response via a detector headset. Detector transmitted field strength was measured with its Yagi-Uda antenna held horizontally level 1.32 m (4.04l, where l is the fundamental signal wavelength) above a concrete floor, using an RF field strength meter (AlphaLab, Inc., Salt Lake City, Utah) in high-pass mode. Total midline horizontal and vertical power density corrected for background was 14.7 mW cm−2 at 8.18 cm (l/4) from the antenna. REFLECTOR-TAG REQUIREMENTS The reflector tag, or transponder, package must have the least possible interference with the movement and behavior of the insect, while enabling detection at least within its normal range travel distances. It was anticipated that a detection range for a BVW of 3 to 4 m would be acceptable, depending on the insect’s location above the ground. The combined receiving and transmitting antenna of the transponder must ideally be simple, lightweight, flexible, and small as possible. A particular constraint is that the transponder should have a minimum of protrusions that could impair insect activity. Figure 1 is a simplified schematic diagram of a reflector tag circuit, of which the Schottky barrier diode is an essential element. The DC path around the Schottky diode, which may include an inductor, conductive strip, or transmission line, provides a pathway for dissipating accumulated charge, allowing the diode to operate at zero-voltage bias (Riley and Smith, 2002). The antenna should be as near as possible to an optimum match with the diode to realize efficient power transfer. Antenna Schottky Diode DC Path Figure 1. Reflector tag schematic diagram. TRANSACTIONS OF THE ASAE THE SCHOTTKY DIODE The Schottky barrier diode is useful for frequency multiplication applications owing to its low junction capacitance and nonlinear characteristic at low signal levels. Models for diode-antenna interaction at high frequencies are complex, since a nonlinear differential equation is encountered (Kanda, 1980). However, the problem is tractable, and Kanda (1980) made some comparisons among analytical and numerical solutions for short-dipole, antenna-driven systems with nonlinear loading, giving transfer function characteristics over a range from 1 kHz to 10 GHz. For our transponder development, it was adequate to assume that the diode impedance had resistive and capacitive (approx. 1.0 pF) components and make use empirical analyses and simulations. A voltage-current characteristic model for the Schottky diode (Kanda, 1980) is: ( αv i (t ) = I s e o (t ) ) −1 (1) where Is is the saturation current (assumed to be 2 × 10−9 A), a = q/nkT ≅ 38 V−1 [q is the electronic charge (1.6 × 10−19 C), n is a dimensionless diode ideality factor (approx. 1.05), and k is Boltzmann’s constant (1.38 × 10−23 J/K)], T is the absolute temperature (approx. 290 K), and vo (t) is the applied voltage. It is presumed that the diode is part of a complete circuit including the antenna. The effect of diode nonlinearity in generating signal harmonics can be seen by application of a simulated incoming radar signal voltage: vo (t )= Vo sin (2πft ) (2) in equation 1, with f = 0.917 GHz and the amplitude (Vo ) chosen arbitrarily to normalize results. Fourier transformation of the resulting i(t) yields the frequency domain plot shown in figure 2. THE ANTENNA Various types of antenna were considered for applicability under the constraints imposed by the insect and the need for the transponder to be as small as possible with acceptable detection range. The antenna selected needed to be tuned to a range of frequencies giving a bandwidth such that incoming and outgoing power could be transferred as efficiently as possible. Antenna configurations are usually expressed in terms of number of wavelengths, with the relation between free space wavelength (l0, m) and the speed of light (c0, m s−1) as: Vol. 48(2): 831−838 Normalized Response Since the BVW cannot fly, its fused wing-cover surfaces provide a favorable base for mounting a transponder. The BVW frequents dark areas under leaves and mulch, where it can find nourishment and protection. Thus, a low-profile antenna is essential to minimize impairment of its movement, which is almost always in a forward direction. Protrusion of transponder components beyond the boundaries of the insect’s body is tolerable provided there is minimum interference with its maneuvering and climbing. The BVW is about 8 to 11 mm long and has a mass of approximately 73 mg. Only limited observations have been made of its load carrying ability, and it was therefore assumed that a transponder weighing more than 30% of BVW body weight could impair its movement, as suggested by the work of Boiteau and Colpitts (2001). Frequency (GHz) Figure 2. Fourier domain plot of Schottky barrier diode normalized current response to an applied sinusoidal voltage at a fundamental frequency of 0.917 GHz (1st harmonic). Harmonics from DC (0th) through the fourth harmonic are shown. The radar detector receives only the 2nd harmonic at 1.834 GHz. λ0 = c0 300 ≈ f f MHz (3) Kraus and Marhefka (2002) and Balanis (1997) are useful references in antenna development. PLANAR ANTENNAS Microstrip or patch-antenna transponders were considered for this application owing to their potential for sizes comparable with the insect and absence of projecting wires. They are a type of planar antenna (fig. 3) used in high-frequency systems owing to their adaptability to small devices and relative ease of fabrication to strict tolerances. However, in the current application with frequencies of 0.917 and 1.834 GHz, there was concern that a patch antenna might still be too large. The shorter dimension (L) of a rectangular, half-wavelength patch antenna would be (notation of Kraus and Marhefka, 2002): L= 0.49λ 0 εr (4) where er is the relative permittivity of a dielectric substrate sandwiched between the conducting patch and the ground plane. Application of equations 3 and 4 to a prototype halfwavelength rectangular patch antenna on a printed circuit board (PCB) with an epoxy-fiberglass substrate of relative permittivity (er ) of 4 and a 1.834 GHz signal gave a value for L of 40.1 mm. With a longer dimension (W = 2L) corresponding to 0.917 GHz and a PCB of 3.2 mm thickness, the antenna mass would be 8.86 g, which would be prohibitive for this application. Use of a higher-permittivity substrate or superstrate and slotted patch and ground planes, to allow meandering of induced currents, could reduce antenna dimensions substantially for fixed operating frequencies. However, the mass of the dielectric and conductors was expected to be excessive, especially for ceramic substrates, with the risk that the unit would become too top-heavy for a BVW to carry. Experimental transponders with patch antennas and Schottky diodes similar to RECCO reflector tags were fabricated, but reduced in size (scaled) in accord with 833 Figure 4. Half-wavelength dipole antenna. Figure 3. Rectangular planar patch antenna. Figure 5. Folded dipole antenna. equation 2. Reduction was accomplished by using a mica substrate with no attempt to slot an antenna of the size needed for a BVW. The effective permittivity (er , eff ) was taken to be the arithmetic mean of the permittivities of the substrate and superstrate. Use of a single, thin layer of mica for the substrate (er = 6) and with air as the superstrate (er = 1) gave a value for er, eff of 3.5, which enabled reduction of tag size to about half that of a RECCO reflector. In trials, this transponder was lacking in both size and performance, with a mass of 94 mg and a range of only about 1 m. Table 1 is a summary of ranging tests for several similar reflector tags using Schottky diodes. Other than preliminary response checks in the laboratory, most ranging trials were done in an outdoor area. Tags were secured to a 40 × 85 mm piece of 2 mm thick, rigid plastic, which served as a dielectric support for the transponder, preventing its distortion due to handling or ground contact. Range measurements were done in a level turf area that was as free as possible from stray electromagnetic fields. Tags were placed on the ground, subjecting them to a relatively severe response test and with ranging performed from the optimum direction according to the transponder antenna pattern. For later tests, with tags using monopole or dipole antennas, this direction would be normal to the antenna axis. When ranging, the operator located the point where a return signal could no longer be detected. Since an operator needed to maneuver the detector at approximately chest level, detection distances were measured horizontally with a surveying wheel from the plane of the forward end of the detector antenna to the transponder. WIRE ANTENNAS Several forms of wire antennas were considered as potentially suitable for tracking the BVW. Among them were the 1/2-wavelength dipole (fig. 4), 1/2-wavelength folded-dipole (fig. 5), 1/4-wavelength monopole over a ground plane (fig. 6), and a magnetic loop (fig. 7). The bold, dark segments in the drawings represent drivepoint locations. The expression relating wavelengths in a vacuum and in a transmitting medium is: λ = λ0 ×V f (5) Figure 6. Quarter-wavelength monopole antenna. Figure 7. Small magnetic loop antenna. 0.95 for wire antennas. Equation 5 is fundamental for singleelement wire antennas and may be scaled to 1/2 or other wavelengths. Dipole and monopole antennas are resonant, such that their feedpoint impedances: Z A = ( Rs + Rloss ) + jX A (6) are completely resistive. A dipole antenna has the advantage of being a single line, as opposed to a plane for a patch antenna. From a size standpoint, at 1.834 GHz, the length of a 1/2-wavelength dipole would be 77.7 mm, large compared to the BVW. A folded dipole antenna, while having greater bandwidth than a dipole, would also be 77.7 mm long. A 1/4-wavelength monopole at 38.3 mm is also large compared to the insect. A magnetic loop, at 15.5 mm in diameter, approaches an acceptable size, but could limit an insect’s access where Vf is the velocity factor, i.e., the ratio of the speed of light in the new medium to that in a vacuum, typically about Antenna Large PCB Small PCB Single-layer mica Mica sandwich 834 Table 1. Scaled transponders. Substrate Dimensions Mass (εr ) (mm) (mg) PCB (4) PCB (4) Mica (6) Mica (6) 59 × 25 25 × 9 22 × 7 22 × 7 3500 1100 94 410 Range (m) 7 1 1 0 Figure 8. Tapped inductor in a tuned circuit. TRANSACTIONS OF THE ASAE Overall Dimensions (mm) Mass (mg) Dipole 78 × 15 30 Folded dipole 15.5 × 5 Antenna Loaded monopole, 3t3-15 mm 19 × 4 Table 2. Wire antenna comparisons. Simulated Simulated Range Z0 Gain (m) (Ω) (dBi) 20 4.6 44.5−j16.4 4.14 4 3.73+j540 −5.4 3.5 6.67−j115 −2.3 to narrow openings. From a purely radiation viewpoint, dipole antennas could be ideal for tracking insects, since they are balanced, can operate without a ground plane, and are generally easy to construct. A 1/10-wavelength dipole at 1.834 GHz would be 15.5 mm long, possibly an acceptable size. However, it might be difficult to mount on an insect because its feedpoint is at the center and approximately half the transponder length would protrude forward. Several constraints make it difficult to synthesize an electrically small and efficient antenna. Feedpoint impedance becomes increasingly capacitive, making XA in equa− tion 6 negative, necessitating inductive loading of the antenna. Two other limitations of small antennas are: (1) low radiation resistance (Rs approx. 0 W), and (2) low efficiency, a problem compounded by low radiation resistance. Radiation resistance seen by the load or source may be matched using an autotransformer configuration, as shown in figure 8, which represents the antenna as a tuned circuit with a tapped inductor. The shortened antenna is the capacitor, and the loading coil is the inductor. The radiation resistance (Rs ) of the antenna can be lumped into the Q of the inductor: R R Q= s = s (7) X L 2πfL which justifies the absence of a resistor in the circuit. Since Rs is small for an electrically small antenna, the apparent resistance (Rs ′) across the tap point can be related to the actual radiation resistance as: n +n Rs ’= Rs 1 2 n1 2 (8) where n1 and n2 are the numbers of turns in the coil, as depicted in figure 8. Thus, the antenna may be tapped to a value that most nearly matches the Schottky diode. After the num− ber of turns is adjusted to achieve resonance, the coil is tapped to provide the required input impedance. Figure 9. Folded dipole far-field radiation pattern. Vol. 48(2): 831−838 Comment 1/10-wavelength loop at feedpoint. 15 mm whip; loaded by three turns on 6-32 machine screw; tap at turn 3. The transponder antenna requirement is similar to highfrequency (HF) mobile units in the intent to provide an electrically small radiator with reasonable efficiency without impeding insect mobility. A HF mobile antenna is often an electrically short whip inductively loaded at the top, center or bottom (Straw, 2002). Typically, a larger inductor is required for center loading, whereas top loading must be capacitive, with greater construction complexity. Thus a simpler base-loaded antenna configuration was chosen for investigation, allowing a smaller inductance. Practical loading inductors typically range from 3 to 8 mm in length, which for a whip less than 20 mm long, is a significant section of the antenna. In computer simulations, loading coils were generated from line segments rather than being represented as complete, wire-wound inductors. In effect, this also generated a small, normal-mode helical antenna topped off with a whip (Kraus and Marhefka, 2002). Copper wire was chosen for the antenna, since it can be easily formed and is available in many sizes, although it is not as durable as steel. However, steel is generally unsuitable for high-frequency circuit or antenna applications due to its poor conductivity compared with copper. Some early experimental prototypes were constructed with 26 AWG copper and, later with 34 AWG copper wire, since fine copper wire is lightweight and flexible. Table 2 provides comparisons from among proposed wire antennas for which computer simulations were done at 1.834 GHz. As indicated earlier, range distances were measured horizontally from the forward end of the transmitting antenna, with transponders on the ground and the detector held at chest level of the operator. The folded dipole has a large inductive impedance component due to its loop configuration. All of the antennas have a toroidal radiation pattern, with the axis along the main radiating element. Figures 9 and 10 illustrate the far-field patterns of a folded dipole Figure 10. Loaded monopole far-field radiation pattern with antenna whip and coil superimposed. 835 and a loaded monopole, respectively. The images are not to scale, representing only the general radiation patterns for either the electromagnetic field or power density. ANTENNA SIMULATIONS Computer simulations were performed using the NEC (Numerical Electromagnetic Code; www.si-list.org/swindex2.html) EZ-NEC software and a free software alternative, 4nec2, to evaluate proposed antenna designs prior to any actual testing. In setting up simulations with these systems, coils were constructed stepwise by means of discrete wire segments. EZ-NEC is less suitable than 4nec2 for simulations requiring a discrete-wire model for an inductor, since 4nec2 supports more discrete-wire segments. A spreadsheet was devised to aid in synthesizing discrete-wire coils of various sizes and precisions. To standardize results, all data shown in table 2 were based on simulations using 4nec2. Figures 9 and 10 were generated with 4nec2, which supports direct 3D rendering. ANTENNA SELECTION Based on the simulations and preliminary physical testing, the base-loaded monopole configuration was identified as most suitable for BVW application from among the wire antennas. This antenna was lightweight, durable, and physically small, and had adequate tracking range for the BVW. It could also be mounted with the whip pointing rearward of the insect, leaving its forward travel relatively unimpeded. Design protocol for transponders with wire antennas was: (1) to place as much wire on the insect as possible until resonance was achieved, and (2) if resonance was not attained, to load the antenna inductively until return signal strength was maximized. TRANSPONDER TESTING WITH INSECTS INSTALLATION AND TRACKING TRIALS Captive insects must be subdued before transponders can be installed. One method is to place the insect in a reduced-temperature environment until its activity is diminished. A simpler way was to release the BVW on a piece of corrugated cardboard and push forceps into the cardboard around its body to restrain it. Then its back was wetted with water as a catalyst for a cyanoacrylate adhesive. After a small drop of adhesive was placed on the diode, it was held in place on the wetted area until secure. One disadvantage of this method is that water tends to cause the adhesive to become milky, possibly obscuring any identifying markings on the transponder or insect. Figure 11 shows a transponder with its loaded monopole antenna mounted on a BVW. The radiation pattern of the transmitting antenna is shown in figure 12, with the antenna’s central metal boom and transverse elements superimposed. The pattern of the Yagi-Uda antenna is predominantly in the “forward” direction, although, as shown in figure 12, minor side and rear lobes occur in the field. Since the electric vector of the electromagnetic field is parallel to the transverse elements of the antenna, the maximum range was obtained when those elements were parallel to the whip of the transponder antenna. The receiving antenna of the detector is also directional, with a strong forward lobe. Detection requires “illumination” by the transmitter and reception of the radiated 2nd harmonic 836 Figure 11. Transponder installed on a BVW. The trailing monopole antenna (next to its shadow) and the diode and loading coil (rear and side of the insect) can be seen. response. Thus, the actual detection pattern is a combination of both antenna fields of the radar detector. It was essential whenever possible to do a 360° sweep when a response signal was first detected to ensure that the main lobes of the detector antennas coincided with the actual direction of the target. A monopole transponder has a toroidal radiation pattern, with the straight antenna along the axis of the torus (fig. 10). Since the antenna whip typically trailed the insect, the weakest signals were received when the operator was directly behind or in front of the insect. When searching for a tagged insect, the operator set the detector sensitivity to its maximum level and held the unit with the Yagi-Uda transmitting antenna elements approximately parallel to the horizon. The antenna was aimed slightly below horizontal, and when a signal was detected, the operator moved toward it while slowly sweeping the unit side to side. As the signal became stronger, the operator reduced the detector sensitivity, allowing the antenna pattern directivity to become more sharply defined. The radar did not have sufficient resolution to precisely locate an insect, requiring visual inspection to locate insects that were be hidden under debris. During the course of experimentation, several weevils were released in a landscaped area for up to 4 days. In these Figure 12. The far-field pattern of the detector’s Yagi-Uda transmitting antenna, with the antenna’s driven, reflector, and director elements superimposed. TRANSACTIONS OF THE ASAE trials, insects were always located, having moved anywhere from a few centimeters to several meters, depending on location and duration of release. One insect carried the first prototype of the 3t3-15 mm loaded monopole antenna for over a month without apparent ill effects. Transponders could be readily removed from test insects with acetone. They remained relatively motionless for a period of time after removal, but soon resumed normal activity. COPPER-PLATED STEEL WIRE ANTENNAS Based on the foregoing work, development of a transponder with a base-loaded monopole antenna using copperplated steel wire was undertaken. The purpose was to take advantage of the ruggedness of steel wire while maintaining the needed conductivity by means of copper coating. This method made use of the “skin effect” wherein conduction occurs primarily at the outer surface of a wire at high frequencies. Steel guitar string wire of 152 mm diameter was chosen for its light weight and spring-like characteristics. Induction coils were cold-formed on a 6-32 machine screw at intervals along a length of wire and stabilized by heating. The wire was then precleaned as necessary and immersed in plating solution in an electroplating unit. A 25 s exposure produced a copper coating of 16 mm thickness. Following final cleaning, antennas with 3-turn coils were cut from the plated wire, with allowance for whips up to about 50 mm length. A Schottky diode was then soldered in place, tapping the coil at its third turn, and the antenna whip was cut to the desired length as for the previous monopole transponders. Transponder mass was typically 27 mg with a 48 mm whip, about 32% of the mass of a BVW. A similar transponder was fabricated with tinned-copper wire (34 AWG) for experimental ranging comparison. Preliminary ranging trials were done with the RECCO detector as for other transponders, with results as summarized in table 3. The range was consistently greater for the copper-plated steel (CPS) than for the tinned-copper (TC) transponder. There may be several reasons for this: First, the monopole whip of the CPS unit was slightly longer than that of the TC unit. The tinning coat on the TC wire may have been less conductive than the copper plating of the CPS unit at the radar frequencies used. Finally, the TC unit can be much more easily distorted or misshapen than the CPS unit, whereby it could have been detuned to some extent. It was concluded that CPS transponders, with their ruggedness, weight, and response characteristics, would be suitable for field tracking studies. CONCLUSIONS It was found that harmonic radar has potential as a research tool for tracking the black vine weevil (BVW). Of the transponder-antenna combinations studied, the Schottky barrier diode with an inductively base-loaded monopole wire antenna was identified as most suitable. Pending further investigation, 15 mm appears to be the minimum useful antenna whip length that would be required. The typical 3 to 4 m detection ranges using a harmonic radar transmitter/receiver set, such as a standard RECCO detector, were considered to be acceptable for the BVW at the present stage of entomological investigations. Overall, the transponder Vol. 48(2): 831−838 Table 3. Comparisons of loaded-monopole transponders with copper-plated steel (CPS) vs. tinned copper (TC) wire antennas. Whip Length Range (m) Location of Transponder (mm) Wire TC CPS CPS TC CPS CPS 40 48 48 40 48 48 0.94 2.0 2.7 6.0 10.0 12.0 On soil surface, in shallow depression On soil surface, in shallow depression On soil surface, at the base of a tree 21.6 cm above soil surface, in open space 21.6 cm above soil surface, in open space 30.5 cm above soil surface, in open space was lightweight and fairly simple to construct, and with a mass of 20 mg was about 1/3 the mass of a BVW. In transponder trials, tagged BVW were successfully located and recovered up to 4 days after release in landscaped areas. Similar transponders constructed with copper-plated steel wire were found to have very acceptable range characteristics in addition to their resistance to deformation and detuning. Design of insect-borne transponders requires careful attention to mass, and to loading and dimensions of antennas. Each insect species can be expected to present unique challenges in terms of its habitat, size, and physiology, and whether it flies, walks, or both. ACKNOWLEDGEMENTS The authors acknowledge helpful discussions with M. J. Sciarini and R. C. Hansen. REFERENCES Balanis, C. A. 1997 Antenna Theory: Analysis and Design. 2nd ed. New York, N.Y.: Wiley. Beerwinkle, K. R., J. D. Lopez, Jr., J. A. Witz, P. G. Schleider, R. S. Eyster, and P. D. Lingren. 1994. Seasonal radar and meteorological observations associated with nocturnal insect flight at altitudes to 900 meters. Environmental Entomology 23: 676-683. Boiteau, G., and B. Colpitts. 2001. Electronic tags for the tracking of insects in flight: Effect of weight on flight performance of adult Colorado potato beetles. Entomologia Experimentalis et Applicata 100(2): 187-193. Capaldi, E. A., A. D. Smith, J. L. Osborne, S. E. 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