Effect of Palm Pollen Extract on Sexual Hormone Levels and Follicle

advertisement
©2014 DAZCo, Danesh Afarand Zistagen Co., IRAN
ISSN: 2252-0805 Quarterly of the Horizon of Medical Sciences 2014;20(3):139-143
Effect of Palm Pollen Extract on Sexual Hormone Levels
and Follicle Numbers in Adult Female BALB/c mice
ARTICLE INFO
Article Type
Original Research
Authors
Hosseini S.E.* PhD,
Mehrabani D.1 PhD,
Razavi F.2 MSc
How to cite this article
Hosseini S.E, Mehrabani D, Razavi
F. Effect of palm pollen extract on
sexual hormone levels and follicle
numbers in adult female BALB/c
mice. Horizon of Medical Sciences.
2014;20(3):139-143.
ABSTRACT
Aims Using chemical drugs to treat disease often is associated with side effects. Recently,
using herbal medicines, which have fewer side effects, has flourished to treat many diseases,
including infertility. This study was done aimed to investigate the effect of palm pollen
aqueous extract on sexual hormones and follicle cells numbers in adult female BALB/c mice.
Materials & Methods This experimental study was done in animal house of Shiraz University
of Medical Sciences in 2012. 40 adult female BALB/c mice were divided into 5 control,
sham and experimental groups receiving 100, 200 and 400mg/kg of the body weight of the
extract groups each with 8 members. Levels of testosterone, estrogen and progesterone
were measured by radioimmunoassay and ovarian follicles numbers were counted after
separation of the ovaries and sectioning and staining using Hematoxylin & Eosin by light
microscopy. The results of hormonal assays were analyzed based on One-way ANOVA and
Duncan’s multiple range tests using SPSS 18 software.
Findings A significant increase was observed at the level of testosterone, estrogen and
progesterone compared to the control group only in the experimental group 3 (receiving
400mg/kg palm pollen extract) (p<0.01). Also, only in the experimental group 3 a significant
increase was observed in the number of secondary follicles (p<0.05) and the number of
antral follicles (p<0.01) compared to the control.
Conclusion 400mg/kg palm pollen aqueous extract increases the amount of sexual hormones
and secondary and antral follicle numbers in adult female mice.
Keywords Pollen; Estrogens; Progesterone; Testosterone
*Biology Department, Sciences
Faculty, Fars Science & Research
Branch, Islamic Azad University,
Shiraz, Iran
1
Pathology Department, Stem Cell
and Transgenic Technology Research Center, Shiraz University of
Medical Sciences, Shiraz, Iran
2
Biology Department, Sciences
Faculty, Fars Science & Research
Branch, Islamic Azad University,
Shiraz, Iran
Correspondence
Address: Biology Department, Sciences Faculty, Islamic Azad University, Kilometer 18th of MarvdashtSadd-e-Dorudzan Road, Iran
Phone: +987143311148
Fax: +987143311172
[email protected]
Article History
Received: October 4, 2013
Accepted: July 7, 2014
ePublished: September 23, 2014
CITATION LINKS
[1] The epidemiological and etiological aspects of infertility in ... [2] Estrogen regulates
pulmonary alveolar formation, loss, and regeneration in ... [3] Resolution of a disordered
region at the entrance of ... [4] Antioxidant activity and phenolic content of various date
palm (Phoenix dactylifera) fruits from ... [5] Possible anti-diarrhoeal effect of the ... [6]
Effect of palm pollen aqueous extract on the sex ratio of ... [7] Comparative nutritive
values of palm saps before and ... [8] Effect of Phoenix Dactylifera pollen on sperm
parameters and ... [9] Prolongation and quality of life for HIV-infected adults treated
with ... [10] In vitro evaluation of the antibacterial activity of ... [11] Protective effect of
extracts from ... [12] The effect of aqueous extract of Pheonix dactylifera pollen grain
on ... [13] Aphrodisiac studies of Trichlepis glaberrima with ... [14] Saliva versus serum
estradiol: implications for ... [15] The Study of the Effects of Dexamethasone During ...
[16] Androgenic potential of aqueous extract of Massularia ... [17] Germline stem cells
and follicular renewal in ... [18] Effects of Phoenix dactylifera on serum concentration of
... [19] The effect of phoenix dactylifera (date-palm) pit powder on ... [20] Phytochemicals
and the breakthrough of traditional herbs in ... [21] Estradiol, esteriol, estrone and
novel flavonoids from ... [22] The gonadotropic activity of palmae in ... [23] The effect
of Phoenix Dactylifera (Date-palm) pit powder on ... [24] Ultrastructural dynamics of
human reproduction, from ovulation to fertilization and ...
All keywords and available citations are linked in this page.
- .#
"/ 0
1 *+,
!"! #$ %! &!'$(
"') . *"" +, *"$ - /0$ /1
7/08 94 #2 3 4 "! *"!" !5 6# *""$
, *"!" *0-01 *7" :!; <# *""$ !"' %?> "! !' :#! 3 %=? => %0
! *<'"' .[=] AB <$ $ %0
#0C' 9, D , # *#0E *<'"'
G!' #F <'C'$&1 *"#,'0 "! 9, .#$'
*'C'$& *9' "! <' H0 "! /0$ :! %
!" *9' "! <I $#J *9'0C'
<I ! %0C LC <$ "! .[K] !' #F <I1
0 !0 *"!" 0) &#M 0 0)
O , 0I1 <'"' % <I1 .[N]
/0$ *"!" Q !' P <#J O' &#M 0
S !' P #0C' ; O' <8 *! !R
V" :"'! W;V ARC < "! /&$T U V" *:!8
+J !' $1 !#X 2' 4Y *"!" 9'Q "!
.[Z] !' :/0C' #F, O' /0$ <8 *:#
*! 0 /1 \" 9'Q "! "! <0& :!;
<"! * <0& \" 9'Q "! :"' - !
"! <0& % . :!, :!; !'( **"0
_` *"! , (Phoenix dactylifera) (
<'b F0, ( *& "! . 4I'C',"
*9 "', :!'$( *!' #M b %0c1
2CB 4 "! .[?] :# %"', d'$ N 0&
F$ +/ S J$ :!& 8 :"e , :# :!! <-$
*I$g1 *:! ( .[f] !' :! F$ $ <!'$
0 +/ S 6! < he _"'i "! ,
<-$ ( B" "! ! U2CB .[j] !' 5V
#0C' 0 l *"1 :0& % :"e , #!k
<-$ _2CB .[n] `' LC $ Yi *7' "! m
o'( %! J$ :!& $! %0p ( :'0 , #!k
HIV r s0 #" 0'0$8 #q
$#0,$8 0C2 *"! ( :'0 .[=> t] #, *0&'
<'b F0, !' .[== j] $I'$8 $Q#q
U" U"v&` ( "! #0E'$u %0$'1 #0E'CC8
%0p %01! P 4Y */, Fe 0
+/ S wF',8 40F0C/ 0 <!,92
.[=N =K] !' <! <$ "! O" *"M 0
%" BALB/c $ !"# *
PhD !
"#$% & % "'(#
PhD 6-4 ,5$ ,
$2 ,3)# *+' ,-./ 012
& % % "!%*/ MSc !
"#$% & % "'(#
:
"# $ %& . !
,-/ ' %# ()% " *%+ %+ % 01 234 5 . "6 %6 7 "- 1) <=> 8 % 9/ %$ "
: ;!
.1 CD BALB/c [email protected] A 1/) [email protected]
C-! GH
*F "& %EF %D) 234 :
BALB/c A % LM .1 CD IJKI % ?84
1>>#6
P%D) 1 '%># )O %$ N " [email protected]
9/ %$ : ;! '1 C%$-#%C%$<-LMM QMM 'IMM
" %T%4 T% '%=) 8 .11 R=S)
U4 1/) [email protected] 1) %$1 A
A " 8:V 6 WXS "Y) 1/) 1
] .1 A P \?%? Z# '5[ 5-=#)
"6%XZ U "8D) : : ^% 2D>
"8D) SPSS 18 : 86C% Z# 5?
">
1>_ :
.1 9-`)
9/ %$ ;! 1>>#6
) J %D) %$ Y>) :
8 (p<M/MI) > 286 '(C%$-#%C%$<-LMM
%># %$ " = %T%4 T% '%=) "+ [email protected] 1) J %D) %$ Y>) 5>d .1 1H
%$ " = (p<M/MI) %: [email protected] 1) (p<M/MN)
.1 1H > 286 %>#
C%$-#%C%$<-LMM 9/ %$ : ;! :
[email protected] 1) 5>d => 8 286 e!
.
[email protected] A %: "+
&7#7 &7#-/ 3&-7# :!"
9;[email protected]/=>/9; :
8
9;?;/=C/@@ :AB/ 8
*
[email protected] :)D' $
'(' & BALB/c % !"#$ % 4, (<C8 GHiperion) NP4 plus 9# /0C +$'(
$#J *9'0C' !#2 .# *0&:#$ (<C8 GDRG) 0,
:! 4, xQR 0 <#J *# w1
*/08$" (0C GLEIYZ) =?=K 9# "! 6'0
U"'$ ‚'0 4, (%'E %00,') H&E 7"
.[=?] # 7" (%1I GNikon)
<'8 4, 9'0C' 7" iV ƒ$ <!'9$
*+5 ƒ$ & "M #0 !"' h'$0 |h&'C',
<'8 Q4 w$" /5 *"8 <'8 r $'"'
/5 SPSS 18 *"8 "/6$ 4, %$! !#b
.# 0Y
! J$ :!& :"e :#,"!) N 5 :& "! </0 "! (p<>/>=) *"!2 +/ (6&'0,6&k0Z>>
:& F$ <I1 <I <' *<'"'
!#2 "! N 5 :& "! %0p .# :#- 9,
9$8 *9'0C' !#2 "! (p<>/>?) '$` *9'0C'
# :#- *"!2 +/ 9, :& F$ (p<>/>=)
.(= 9#)
&7#7 &7#-/ &-7# ,3&'3 "'# EF# (% #$
BALB/c K3A' )7 IJ I. ,3)!. G H'
P +2L'9== 2 %N) 9 L2M 3% )7$+ K3
"2 P +2L'@== 2 %N) @ L2M (R1 "2
(R1 "2 P +2L'C== 2 %N) ; L2M (R1
- )
*+
, )
*+
% )
*+
'
#
&"
(7.L'2!/) .
&&+ .
=/SS±=/;S* =/[email protected]±=/=? =/[email protected]±=/;= =/;S±=/=? =/[email protected]±=/@@
(7.L'2!/) .
& .
9S/?=±=/;9* 9U/[email protected]±9/CC 9U/C=±=/9> 9;/C=±=/;? [email protected]/US±=/@=
(7.L'2!/) .
&
/ .
*
9>/=U±=/S; 9U/CS±=/;C 9;/>=±9/=C >/[email protected]±=/U; S/S=±=/99
#0 1+
9/T>±=/CT 9/UT±=/T; 9/[email protected]±=/UU 9/@±=/@U
9/;±=/@=
23 #0 1+
@/9=±=/CU** 9/?C±=/9S 9/[email protected]±=/@U 9/>U±=/;U 9/S±=/@S
#
&4 #0 1+
@/==±=/@=* 9/;C±=/;C 9/C±=/99 =/SU±=/CU =/T±/>=
p</ *
p< / **
:!& $! 8 :"e , !! <-$ +z1 % iV ƒ$
"!2 +/ S 6&'0,6&k0Z>> ! J$
Q Horizon Med Sci
Vol. 20, Iss. 3, 2014
"! <'( #0l0C 6 %0c1 %0'FC8 +/ S J$ :!&
.[=Z] !' :# ! $8 <#J , 7'
! *"0 <"! * "! <0& :!; , 5$8 <0& +01 <M0$ !#V :!' 9'2 *- x' "!
" *"0 <"! * "! %0 x "!
"! k00 *"! F$ y"' ' #"
' "! <0& _` " _"q 0( *9
DF$ */ F$ y"' :# *-0
*$'$ *""$ <"! "! , 00 *"! !
7" ' %0p #$0& "M :!; !"' <$ "!
% ! _$'0V "! 'M#b :z *"" _"#M *!eM
</0 J$ :!& $! 8 :"e ` " h# {0RY
%0p <I1 <I <' *<'"'
.# 65$ LC :! *7' "! $#J *9'0C' !#2
0 /1 6' :-$! _$'0V $( "! qV 5 +z1
< LC :! BALB/c 7' Z> .# 65$ =Nt= 9 "!
/1 6' :#-$! _$'0V 7"1 /, 6&N>|N? FR
O KK±K°C *! "! o'eJ } 4 "! 0 0
$'$ .#$# *"#$ " =K " =K
*! :#,"! U5 # 9, n :& ?
:!& 8 :"e <# < 6&'0,6&k0Z>> K>> =>>
_"'i _$'0V +z1 :"! _# 9'Q "! .#$# 0R J$
.#$!, :!; $#08 s8 vX !#Y$
V BR s8 "! : :!& # J$ :!& 8 :"e 0 U
s'( Z>°C _"V *"% *" R0M!N> _# :#
*"#$ Zn #2 .!' iV (' ~'J # :!
0JF :! 4, hi iV ~'J :-8 *' "!
.# *"8x :"e , </0 4-( U""
Y # :& #$ "M *"0 0 Y 9, :&
<' 4I'C'/0 6 (/$I :"!) :"K= M;i<"! /'5
/0$ J$ :!& :"e 5 *:& .#& "M "! 9uV
" K= _# * " J$ :!& 8 :"e :#%002 *!
w1 +8 :"! <1 "! .#$!, "! M;i<"! _"'i
<'( .#8 *0&<'( $8 €M _$'0V <!'$7'0
J" 8 "'Q +8 *C'C "! _$'0V :#0
wl # *"#$ :-8 *! "! JC 0- 6
0 # +8 C'C "# <'( JC s' 0
$8 6 R0M!K> _# R0M!"!"!N?>> I'0;$ :!
<I <' *<'"' </0 .# *#
:! :!; (RIA) '$''!" 7" <I1
" {Q ( :!& "! !'' 4"c#0 40C1#0
<' #F x$ ,!";C8|? /$8 0C2
<' 50$ "! !' "! <'"#0*!
5 $ "! :# #F <'"#0*! *,
5$8 .[KN] !' <'( "! <' <'"' )X +/
w1 #$' ‡J- #C' # "! 0C *9'0C' !#2 ,
!#2 "! 00ˆ < , # #,$ 00ˆ #C'
C # #$ !' "0 *:& "! 0C *9'0C'
S J$ :!& _2CB ( ƒ$ r $8 '
S #$'k "',v *<'"' !' LH FSH +/
.[=j =K] #$' *<'"' $#J *9'0C' #"
, FSH P +/ S ( _F0, "! !'' %0$'1
*9'0C' '$ #" 9'! P 0) "! +R$
9'0C' #" LH €0, "! FSH .[KK] !' !"! 9$8
0R \1 xM "! #, 4Y " *"v&4J FM
-0 *:#$0& ~' 'C'$& *9' "! LH +/ LH +/ [KZ] r'C'', *9' F$ <8
*9' "! " *!#2 C' (! $"601 *0 0C2
#0C' </0 LH +/ #2 .#, *#$:" 'C'$&
0, C l0C'; 0C2 %0p _;* 9''
+/ S +/ 'C'$& *9' "! C' (!
9'0C' #0C' +/ DTV , !' 9' 0m
.#, 0' " *<'"'
U0, 0 U"'! <'k +z1 % U!#Y %002 U 0$ !"' _$ <!'F$r!"! k$'"' +5
.!'$ :" J$ :!& :"e :#!0- k00 _F0, {0M!
"!2 +/ F +z1 % iV ƒ$ ' ! :#,"! 5 :& "! 9$8 '$` *9'0C' !#2
-0 0 _R0RY 65$ <' 6&'0,6&k0Z>>
d'$ ( <"! * 0$ !"' *"! <' <8
+/ %0p <$ "! *$'$ *""$
.!'$ :!; ! _$'0V 7"1 /, "! #0C' !#2
+/ S 6&'0,6&k0Z>> ! "! J$ :!& 8 :"e
'$` *9'0C' !#2 %0p *<'"' </0
.!' LC :! *7' "! 9$8
#$! € !'( CR <&#'$ : 40$Iw$ *"‰ *!0 *9' _R0RY /, #
:I1 % !' _$ , 0 /1 6' :-$!
.#$ $!"#M - #$!'$ " R0RY
*9'0C' <' <I1 <I *<'"'
:!! <-$ " % "! ' .!' 9$8 '$`
$#J *9'0C' !#2 +/ S J$ :!& , #
M <I1 <I *<'"' +/ w1 !'
*#0E *#0E'$u _F0, J$ :!& . 0+01
40$I"#$8 *<'"' P 4Y S *#0l0C 0$'1
+/ qV +z1 "! DTV [=f] #$' LH
. LH +/ 0C! <I1 <'"'
"! 9'! </0 +/ S ( , #!k <-$ _2CB
S %0p <#J #M C *7' :& K "! / "#R C :! *7' "! <I1 +/
( …J *+J .[=j] !' <#J #M *7'
*<'"' </0 #$' , 0& *9 *"!
:! Yi *7' "! .[?] # ! :#/ ` *#0E
</0 +/ S J$ :!& :"e , :!! <-$ /0$ LC
</0 , CV "! !' <I <I1 u1
J$ :!& $! :"e "! .[=n] 0` <# LH FSH
*"! , %0" %0††E', 9'0 | 0)$ F0,
+/ DTV [=t] !"! !' # <I 0C2
6&'0,6&k0Z>> 5 :& "! <I </0
. _F0, % !'
+/ S ( :!& :"e , #!k <-$ U! 2CB
+/ DTV G[K> =K] !' LH FSH <'"'
0` <' " <I1 <I *<'"'
#!k <-$ *! _2CB .$! "',v *<'"'
C' $ C'! _F0, *V J$ :!& ,
)X +/ %0p *"" _"#M +/ S , iV ƒ$ ' .[K=] !' 9'! <'"' u1
{"/ , #!k <-$ *! 2CB +z1 % <' *<'"' +/ S J$ :!& M;i<"!
!' :! $ w ! "! %01!$'& 9'!
+/ S b /5 {Q J$ :!& .[KK]
" %0'FC8 *'0, x! ! hQ !' !8 sb *#0
!8 sb *#0 +/ DTV #! +/ /0$
4 % #, M" %0'FC8 9e "! <I1
%0'FC8 <I1 9e +, %0'FC8 x! +/ hQ
9e !8 sb *#0 +/ ! hQ <'"' PB </0 +/ S ! %0'FC8
.#$' <I1
+/ {Q J$ :!& "! 6'0!, *" 0)$ F0, !'
#0C' +/ S $I"#0! #0E ,"#0 |=j /'0
0)$ F0, .[KK] !' #0E 0C' <'
/01 .' BALB/c -% &
'( )* +, - ! "# $ 11- Al-Qarawi AA, Mousa HM, Ali BH, Abdel-Rahman H,
El-Mougy SA. Protective effect of extracts from dates
(Phoenix dactylifera L.) on carbon tetrachloride-induced
hepatotoxicity in rats. Intern J Appl Res Vet Med.
2004;2(3):176-80.
12- Abedi A, Parviz M, Karimian SM, Sadeghipour Rodsari
HR. The effect of aqueous extract of Pheonix dactylifera
pollen grain on sexual behavior of male rats. J Physiol
Pharmacol Adv. 2012;2(6):235-42.
13- Padashetty SA, Mishra SH. Aphrodisiac studies of
Trichlepis glaberrima with supportive action from
antioxidant enzymes. Pharm Biol. 2007;45(7):580-6.
14- Tivis LJ, Richardson MD, Peddi E, Arjmandi B. Saliva
versus serum estradiol: implications for research studies
using postmenopausal women. Prog Neuropsychopharmacol
Biol Psychiat. 2005;29(5):727-32.
15- Moshfeghi M, Hosseini SE, Vahdati A, Moshfegh Z.
The Study of the Effects of Dexamethasone During
Pregnancy on development and Function of Ovary Tissue in
Offspring of First Lineage Rats. Q J Anim Physiol Dev.
2014;7(2):23-30.
16- Yakuba MT, Akanji MA, Oladiji AT, Adesokan AA.
Androgenic potential of aqueous extract of Massularia
acuminata (G. Don) Bullock ex Hoyl. Stem in male Wistar
rats. J Ethnopharmacol. 2008;118(3):508-13.
17- Jahnson J, Canning J, Kaneko T, Pru JK, Tilly JL.
Germline stem cells and follicular renewal in the postnatal
mammalian ovary. Nature. 2004;428(6979):145-50.
18- Moshtaghi A, Johari H, Shariati M, Amiri J. Effects of
Phoenix dactylifera on serum concentration of estrogen,
progesterone and gonadotropins in adult female rats. J
Rafsanjan Uni Med Sci. 2010;9(2):117-24. [Persian]
19- Shariati M, Sharifi E, Kaveh M. The effect of phoenix
dactylifera (date-palm) pit powder on testosterone level and
germ cell in adult male rats. Sci J Zanjan Uni Med Sci.
2007;15(61):21-8. [Persian]
20- Adimoelja A. Phytochemicals and the breakthrough of
traditional herbs in the management of sexual dysfunctions.
Int J Androl. 2000;23(Suppl 2):82-4.
21- Abbas FA, Ateya AM. Estradiol, esteriol, estrone and
novel flavonoids from date palm pollen. Aust J Basic Appl
Sci. 2011;5(8):606-14.
22- El Mougy SA, Abed Aziz SA, Al-Shanawany M, Omar
A. The gonadotropic activity of palmae in mature male rats.
Alexandria J Pharm Sci. 1991;5:156-9.
23- Shariati M, Sharifi E, Kaveh M. The effect of Phoenix
Dactylifera (Date-palm) pit powder on testosterone level and
germ cells in adult male rats. ZUMS. 2007;15(61):21-8.
24- Familiari G, Heyn R, Relucenti M, Nottola SA,
Sathananthan AH. Ultrastructural dynamics of human
reproduction, from ovulation to fertilization and early
embryo development. Int Rev Cytol. 2006;249:53-141.
Q Horizon Med Sci
Vol. 20, Iss. 3, 2014
C%0 %0$'M r +z1 % 1 : :-$! }u( 0, "! 0) -8 _$'0V !"' "!
.#0" €'e
. :#-$ 7"/& <&#'$ O' U!"' : :# %0 <&#'$ O' AQ % kC x : .
1- Aflatoonian A, Seyedhassani SM, Tabibnejad N. The
epidemiological and etiological aspects of infertility in Yazd
Province of Iran. Iran J Reprod Med. 2009;7(3):117-22.
2- Massaro D, Massaro GD. Estrogen regulates pulmonary
alveolar formation, loss, and regeneration in mice. Am J
Physiol Lung Cell Mol Physiol. 2004;287(6):1154-9.
3- Grishkovskaya I, Avvakumov GV, Hammond GL, Muller
YA. Resolution of a disordered region at the entrance of the
human sex hormone-binding globulin steroid-binding site. J
Mol Biol. 2002;318(3):621-6.
4- Biglari F, AlKarkhi AF, Easa AM. Antioxidant activity
and phenolic content of various date palm (Phoenix
dactylifera) fruits from Iran. Food Chem. 2008;107(4):163641.
5- Al-Taher AY. Possible anti-diarrhoeal effect of the date
palm (Phoenix Dactylifera L.) spathe aqueous extract in
Rats. Sci J King Fasial Univ Basic Appl Sci. 2008;9:121-37.
6- Hosseini SE, Mehrabani D, Razavi FS, RafieiRad M.
Effect of palm pollen aqueous extract on the sex ratio of
offspring in mice strain BALB/c. Q Res J Lorestan Uni Med
Sci. 2013;15(2):121-8. [Persian]
7- Barh D, Mazumdar BC. Comparative nutritive values of
palm saps before and after their partial fermentation and
effective use of wild date (Phoenix sylvestris Roxb.) sap in
treatment of anemia. Res J Med Sci. 2008;3(2):173-6.
8- Bahmanpour S, Talaei T, Vojdani Z, Panjehshahin MR,
Poostpasand A, Zareei S, et al. Effect of Phoenix Dactylifera
pollen on sperm parameters and reproductive system of
adult male rats. IJMS. 2006;31(4):208-12.
9- Burgoyne RW, Tan DH. Prolongation and quality of life
for HIV-infected adults treated with highly active
antiretroviral therapy (HAART): A balancing act. J
Antimicrob Chemother. 2008;61(3):469-73.
10- Jassim SA, Naji MA. In vitro evaluation of the
antibacterial activity of an extract of date palm (Pheonix
dactylifera L.) pits on a pseudomonas phage. Evid Based
Complement Alternat Med. 2010;7(1)57-62.
Download