MBIO 4600/4602 Molecular Genetics of Prokaryotes
Fall 2013
Instructor: Dr. Ann Karen Brassinga [Tel: 799-8457; Email: Karen_Brassinga@umanitoba.ca]
Lecture Time and Room: 11:30 am – 12:20 pm MWF Rm. 315 Buller Bldg.
Office Location and Hours:
Rm. 418 Buller; generally 12:30-1:30 p.m. daily or by appointment.
Required textbook: Molecular Genetics of Bacteria 4TH Ed., Snyder, Peters, Henkin and Champness
One copy is on 1-hr reserve in Science and Technology Library.
 Online resource Bacterial and Bacteriophage Genetics (2006) by Birge
http://www.springerlink.com/openurl.asp?genre=book&isbn=978-0-387-23919-4
 Your Microbiology A/1010/2020 textbook also has good background information.
Lecture material: Lecture notes and supplementary material will be posted on Angel
MBIO 4600 Laboratory: Dr. L. Cameron 414B Buller (office); The lab manual will be handed out in class and a copy
can be found on the Microbiology website: (http://umanitoba.ca/science/micro300400labs/).
NOTE: MBIO 4602 is NOT accepted as a 4000-level MBIO course for students currently registered in four-year
Honours or Majors programs in Microbiology, Biochemistry or Genetics. No exceptions! Remember that you are
responsible for ensuring that your courses meet degree requirements.
Date
Lecture Content*
Friday, September 6
Introduction
Monday, September 9
Review – Nature and Structure of DNA
Wednesday, September 11
Review – Replication
Friday, September 13
Review – Transcription
Monday, September 16
Review – Transcription Regulation and Translation
Tuesday, September 17
MBIO 4600 Lab 1– The lac system; Lab 1 Report due Oct. 1
Wednesday, September 18
Conjugation
Friday, September 20
Conjugation
Monday, September 23
Conjugation
Tuesday, September 24
MBIO 4600 Lab 2 – Conjugation; Lab 2 Report due Oct. 15
Wednesday, September 25
Transformation
Friday, September 27
Transformation
Monday, September 30
Term Test I – 8% of final course grade (Sept. 9 – Sept. 23 Lectures inclusive)
Tuesday, October 1
MBIO 4600 Lab 3 – Part 1:Transformation – Plasmid DNA Isolation and Analysis
Wednesday, October 2
Transduction
Friday, October 4
Transposition
Monday, October 7
Transposition
Tuesday, October 8
MBIO 4600 Lab 3 – Part 2:Transformation – Competent Cell Preparation
Wednesday, October 9
Transposition
Friday, October 11
Replication and Repair
Monday, October 14
Thanksgiving Day – no classes
Tuesday, October 15
MBIO 4600 Lab 3 – Part 3:Transformation: Xgal detection; Lab 3 Report due Oct. 29
Wednesday, October 16
Replication and Repair
Friday, October 18
Replication and Repair
Monday, October 21
Recombination
Tuesday, October 22
MBIO 4600 Lab 4 – P1 Generalized transduction; Data due Oct. 25; Report due Nov. 5
MBIO 4600/4602 Molecular Genetics of Prokaryotes
Wednesday, October 23
Recombination
Fall 2013
Friday, October 25
Recombination
Monday, October 28
Term Test II - 12% of final course grade (Sept. 25 – Oct. 18 Lectures inclusive)
Tuesday, October 29
MBIO 4600 Lab 5 - Transposition: 1098 Titration
Wednesday, October 30
Phage Genetics
Friday, November 1
Phage Genetics
Monday, November 4
Phage Genetics
Tuesday, November 5
MBIO 4600 Lab 5 – Tn10 transposition; Data due Nov. 8; Report due Nov. 15
Wednesday, November 6
Plasmid Genetics
Friday, November 8
Plasmid Genetics
Monday November 11
Remembrance Day Observed (No classes)
Wednesday, November 13
Bacterial Tools for Genetic Engineering - Last day for voluntary withdrawal
Friday, November 15
Bacterial Tools for Genetic Engineering
Monday, November 18
Term Test III - 10% of final course grade (Oct. 21 – Nov. 8 Lectures inclusive)
Wednesday, November 20
Molecular mechanisms of pathogenesis
Friday, November 22
Molecular mechanisms of pathogenesis
Monday, November 25
Molecular mechanisms of pathogenesis
Tuesday, November 26
MBIO 4600 Lab exam
Wednesday, November 27
Molecular mechanisms of pathogenesis
Friday, November 29
Molecular mechanisms of pathogenesis
Monday, December 2
Molecular mechanisms of pathogenesis
Wednesday, December 4
Review for exam – MBIO 4602 Assignment due
TBA
FINAL EXAM (CUMULATIVE) – 50% of final course grade
* The lecture content and schedule is tentative and may be subject to change
Marking:
MBIO 4600: Term tests (30%), Final Exam (50%), Laboratory (20%)
MBIO 4602: Term tests (30%), Final Exam (50%), Assignment (20% - due by Wednesday, December 4)
There will be no deferred term test. You must email me a valid reason (illness, family emergency, etc.) within one week
of the missed term test. Once your reason is validated, then the marks of the missed term test will be added to your final
exam. No emailed valid reason = no transference of marks and you will receive a mark of “0” on the missed term test.
Note that the final exam schedule may be worse than your midterm schedule and for most students a 60-80% final is not
less stressful than one worth 50%. The Faculty of Science regulations apply to all missed final exams. MBIO4600: You
must pass the lab to pass the course. MBIO4602: Late assignments will not be accepted.
NOTE: Any e-mails must be sent to me from your university email account. E-mails sent to me from an email
account other than the University of Manitoba account will automatically be deleted.
Academic dishonesty
Guidelines stated in your calendar regarding University policy with respect to academic dishonesty (including plagiarism
and cheating) and behaviour and absence from final exams will be followed.
Also see the Faculty of Science website (http://umanitoba.ca/faculties/science/student/webdisciplinedocuments.html).
Please ask if you have any questions.