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O
OH membranes by several Good buffers, including ADA , has been reported.
5 A study of the chelation of H + , Ca 2+ , and Mg various buffers, including ADA , has been published.
6
2+ to
H
2
N
O
N
ADA has been shown to interfere with color development in the bicinchoninic acid assay for protein quantitation.
7 The use of ADA in the isoelectric focusing of proteins in immobilized pH gradients has been described.
8
O OH
Molecular Formula:
Molecular Weight:
Appearance:
C
6
H
10
190.2
N
2
O
5
White Powder
Solubility ( 10% in
1n n a
OH ): Clear and Colorless
Solution
Assay ( M in
.
):
Loss on Drying ( M ax
.
):
Heavy Metals ( M ax
.
):
98.0%
1.0%
10 ppm
This product is soluble in 1 M NaOH ( 160 mg
/ ml
), yielding a clear and colorless solution.
This product is solely for Research and Development use.
It is not for drug, household, or any other uses. Please review the Safety Data Sheet for information regarding risks and safe handling practices.
ADA is a zwitterionic buffer used in biochemistry and molecular biology. It is one of the “ Good Buffers ” developed in the 1960 ’s to provide buffers in the pH range of 6.15
to 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of ADA and its physical properties.
1 The useful range of ADA
B uffer
in aqueous solution is 6.0
to 7.2
.
1. Good, N.E., et al., Hydrogen Ion Buffers for Biological research. Biochemistry , 5(2) : 467 - 477, (1966).
2. Robinson, J.D., Davis, R.L., Buffer, pH and Ionic strength effects on the
(Na + + K + )-ATPase. Biochim. Biophys. Acta , 912(3) : 343 - 347, (1987).
3. Pietzkowski, Z., et al., Cellular activities associated with the transition of chick embryo Fibroblasts from stationary to proliferation state. Folia Histochem. Cytobiol.
, 27(3) :183 - 196, (1989).
The effect of ADA on the activity of dog kidney
( Na + + k +
)ATPase activity has been investigated.
2 ADA has been used in a protein-free medium for supporting chick embryo fibroblasts.
3 Cardiac muscle contraction in various buffers, including ADA , has been studied.
4 The inhibition of
γ-aminobutyric acid receptor binding to rat brain synaptic
4. Bers, D.M., et al., Citrate decreases contraction and Ca current in cardiac muscle independent of its buffering action. Am. J. Physiol.
, 260(5) : C900 - C909, (1991).
5. Tunnicliff, G., Smith, J.A., Competitive inhibition of γ-Aminobutyric Acid receptor binding by N-2-Hydroxyethylpiperazine-N’-2-о-Ethanesulfonic
Acid and related buffers. J. Neurochem.
, 36(3) : 1122 - 1126, (1981). page 1

6. Durham, A.C., A survey of readily available chelators for buffering Calcium Ion concentrations in Physiological
Solutions. Cell. Calcium , 4(1) : 33 - 46, (1983).
7. Kaushal, V., Barnes, L.D., Effects of Zwitterionic Buffers on measurement of small masses of Protein with Bicinchoninic acid. Anal. Biochem.
, 157(2) : 291 - 294, (1986).
8. Righetti, P.G., et al., Immobilized pH gradients: effect of
Salts, added carrier Ampholytes and voltage gradients on
Protein patterns, Electrophoresis , 9(2) : 65 - 73, (1988).
NBC warrants that its products will conform with the information provided in this information sheet, though the Purchaser must determine the suitability of the products for their particular usage needs. Additional terms and conditions may apply.
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