University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Eastern Pine and Meadow Vole Symposia Wildlife Damage Management, Internet Center for 3-4-1981 EXPERIMENTAL RESULTS OF CHLOROPHACINONE GROUND SPRAYS IN NORTH CAROLINA William T. Sullivan Jr. North Carolina State University, Raleigh, North Carolina Follow this and additional works at: http://digitalcommons.unl.edu/voles Part of the Environmental Health and Protection Commons Sullivan, William T. Jr., "EXPERIMENTAL RESULTS OF CHLOROPHACINONE GROUND SPRAYS IN NORTH CAROLINA" (1981). Eastern Pine and Meadow Vole Symposia. Paper 60. http://digitalcommons.unl.edu/voles/60 This Article is brought to you for free and open access by the Wildlife Damage Management, Internet Center for at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Eastern Pine and Meadow Vole Symposia by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. EXPERIMENTAL RESULTS OF CHLOROPHACINONE GROUND SPRAYS I N NORTH CAROLINA William T. S u l l i v a n , Jr. North' C a r o l i n a S t a t e U n i v e r s i t y Raleigh, North C a r o l i n a 27650 ABSTRACT: F i e l d experiments w i t h chlorophacinone (CPN) ground s p r a y s seem t o b e more e f f e c t i v e i n c o n t r o l of p i n e v o l e s i n North C a r o l i n a o r c h a r d s when t h e p e r c e n t a g e g r a s s cover under t r e e d r i p l i n e s i s h i g h . P r e l i m i n a r y l a b o r a t o r y r e s u l t s seem t o confirm t h i s o b s e r v a t i o n . Our group h a s c a r r i e d out a number of f i e l d t r i a l s of t h e e f f i cacy of ground s p r a y s f o r c o n t r o l l i n g p i n e v o l e s i n o r c h a r d s . I n r e viewing t h e s e tests w i t h s p e c i a l r e f e r e n c e t o i n c o n s i s t e n c y of r e s u l t s w i t h chlorophacinone (Hayne 1977) a n a p p a r e n t r e l a t i o n s h i p t o t h e amount of g r a s s cover was n o t e d , and l a b o r a t o r y t r i a l s were s t a r t e d t o test t h i s q u e s t i o n . METHODS: The f i e l d experiments were c a r r i e d o u t i n privately-owned Each experimental p l o t of about o r c h a r d s i n Henderson County, N.C. 2.0 a c r e s c o n t a i n e d a c e n t r a l d a t a a r e a and a b u f f e r zone; t h e b a s i c d e s i g n was d e s c r i b e d by S u l l i v a n and Hayne (1978). Vole a c t i v i t y was monitored b e f o r e and a f t e r t r e a t m e n t by l i v e t r a p p i n g and t h e a p p l e s i g n t e s t . Blood c o a g u l a t i o n times were a l s o recorded b u t a r e n o t r e p o r t e d h e r e . Toxic ground s p r a y was a p p l i e d by u s i n g a n a n g u l a r boom t h a t d i s t r i b u t e d t h e m a t e r i a l evenly from t h e t r e e t r u n k o u t t o t h e d r i p l i n e . An o p e r a t i n g p r e s s u r e of 125 t o 135 p s i was used; t h i s i s lower t h a n recommended b u t we f e e l t h a t u s i n g t h i s lower p r e s s u r e reduces d r i f t and keeps most of t h e t o x i c a n t under t h e t r e e . The a p p l i c a t i o n r a t e was v a r i e d i n a s t u d y of t h i s f a c t o r . Laboratory t e s t s were c a r r i e d o u t i n m e t a l boxes u s i n g methods d e s c r i b e d by Davis e t a l . (1980) w i t h t h e d i f f e r e n c e t h a t i n some boxes sod w i t h a v i g o r o u s growth of g r a s s was used i n s t e a d of b a r e s o i l . I n one s e t of boxes c o n t a i n i n g sod we added a measured amount of w a t e r t o s i m u l a t e r a i n f a l l (325 mllday w i t h 12 a n i m a l s , 700 ml/day w i t h 1 0 ) . RESULTS: Table 1 shows t h e r e s u l t s of t h e f i e l d tests. The h i g h e r t h e p e r c e n t a g e ground cover, t h e more e f f e c t i v e t h e apparent c o n t r o l . The l a b o r a t o r y r e s u l t s (Table 2) a r e c o n s i s t e n t i n t h a t a t t h e same a p p l i c a t i o n l e v e l , m o r t a l i t y seemed t o b e h i g h e r w i t h sod. Use of s i m u l a t e d r a i n f a l l seemed t o have l i t t l e e f f e c t on t h e outcome. DISCUSSION: These r e s u l t s i n d i c a t e t h a t chlorophacinone ground s p r a y i s most e f f e c t i v e i n t h e presence of v e g e t a t i o n a l ground cover, i n t h i s c a s e , g r a s s . H o r s f a l l e t a l . (1974) observed t h a t t h e i n g e s t i o n of t h i s l e t h a l a g e n t by mice may b e enhanced by t h e presence of f o r b s i n t h e t r e a t e d g r e e n e r y . Both o b s e r v a t i o n s a r e c o n s i s t e n t w i t h t h e l a b e l a d v i c e n o t t o s p r a y b a r e ground. A t p r e s e n t we conclude t h a t where t h e r e i s l i t t l e o r no v e g e t a t i o n under t h e t r e e s , chlorophacinone ground s p r a y may n o t b e expected t o p r o v i d e good c o n t r o l of v o l e s . There may b e need t o look a t o t h e r ground s p r a y s under t h e s e same c o n d i t i o n s . Table 1. F i e l d t r i a l s of chlorophacinone ground s p r a y l i s t e d i n o r d e r of amount of g r a s s cover. Application p e r sprayed acre gal lb ai Percent activity apple sign test Grass percent cover mean height in pre post Number of v o l e s live-trapped postpretreatment treatment marked marked unmarked To q u a l i f y f o r i n c l u s i o n i n t h i s t a b l e t h e t e s t must have had e i t h e r 4 animals marked and r e l e a s e d b e f o r e t r e a t m e n t o r 10 p e r c e n t a c t i v e s t a t i o n s with the apple s i g n t e s t . *Caught i n f i r s t 48 h o u r s a f t e r s p r a y i n g ; no c a p t u r e a f t e r 72 hours. Table 2. L a b o r a t o r y tests of t o x i c i t y t o p i n e v o l e s of chlorophacinone a p p l i e d t o b a r e s o i l and t o sod. Treatment cover Bare soil lb a i per acre Number of v o l e s ( d i e d l t o t a l ) treated control 19/22 014 0.2 0.4 Sod, no water 0.2 Sod, w i t h water 1.2 LITERATURE CITED Hayne, D. W. 1977. S u r v i v a l r a t e s of p i n e v o l e s i n North C a r o l i n a o r c h a r d s . Pages 70-75 & R. E. Byers, ed. Proc. F i r s t E a s t e r n P i n e and Meadow Vole Symposium, Winchester, V i r g i n i a . H o r s f a l l , F . , R. E. Webb and R. E. Byers. 1974. Dual r o l e of f o r b s and r o d e n t i c i d e s i n t h e ground s p r a y c o n t r o l of p i n e mice. Pages 112-216 & Proc. S i x t h V e r t e b r a t e P e s t C o n t r o l Conf., Anaheim, C a l i f . Davis, D. H., W. T. S u l l i v a n , Jr. and D. W. Hayne. 1980. R e s u l t s of a b i o a s s a y t e c h n i q u e f o r ground sprayed r o d e n t i c i d e s . Pages 58-61 i n R. E. Byers, ed. Proc. Fourth E a s t e r n P i n e and Meadow Vole Symposium, H e n d e r s o n v i l l e , North C a r o l i n a . S u l l i v a n , W. T . , J r . and D. W. Hayne. 1978. An e x p e r i m e n t a l comparison of v o l e c o n t r o l methods. Pages 49-51 & R. E. Byers, ed. Proc. Second E a s t e r n P i n e and Meadow Vole Symposium, B e l t s v i l l e , Mary land.