13TH TRANSGENIC
TECHNOLOGY MEETING
March 20–23, 2016 | Prague, Czech Republic
MEETING PROGRAM
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
CONTENT
Welcome
5
Committees & Organizers
6
Scientific Program
8
Posters
16
Guidelines for Presenters (Oral and Poster)
37
General Information
38
Social Program
39
Exhibition
40
Venue & Exhibition Floor Plan
40
List of Exhibitors
41
Sponsors/ Exhibitors
42
Author Index
55
TT2016 Participants List
60
Map of Prague
70
3
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
WELCOME
New actuator with
extended intensity range
Dear Colleagues,
As chairman and on behalf of the organising and scientific committees,
it is my pleasure to welcome you to the 13th Transgenic Technologies
meeting (TT2016), which is being held on behalf of the International
Society for Transgenic Technologies (ISTT) in Prague, Czech Republic,
one of the most beautiful cities in the world.
e meeting is being hosted by three Czech research institutes in association with the
Czech Academy of Sciences: Czech Centre for Phenogenomics, Institute of Molecular
Genetics of the ASCR, v. v. i. and Biotechnology and Biomedicine Center of the Academy
of Sciences and Charles University in Vestec (BIOCEV). All three are leading centres in
the Czech Republic.
Our TT2016 programme is filled with presentations from leading scientists who have
made valuable contributions to the transgenic technologies field and our poster
session will also showcase the latest developments in technologies within the
community. I would like to say a special thank you to all presenters for agreeing to
share your research, knowledge and expertise with the wider scientific community.
e field of transgenic technologies has been revolutionised by advances in
programmable nucleases, especially CRISPR/Cas9 and our ‘World of Nucleases:
discovery and development’ session will detail the history of the CRISPRs, the newest
developments and applications, and the prospects and potential in the nuclease field.
In addition to this session, we will have two Orbis pictus lectures presented by omas
Boehm and Richard Behringer. ese sessions have been designed as educational
overviews and will focus on the technology development.
Impetus
It is also my pleasure to welcome Dr Pablo Ross to this meeting. Dr Ross will receive the
4th ISTT young investigator award and will give a presentation detailing his scientific
achievements and his research.
Successful micromanipulation with the Eppendorf PiezoXpert®
Are you performing difficult injections
or working with demanding samples
(e.g., oocytes of large animals)? Or do
you face the challenge of increasing
the survival rate of your manipulated
cells? Piezo-assisted micromanipulation can help improve the outcome of
your experiments.
The Eppendorf PiezoXpert is an excellent tool for applications, such as:
> Transfer of cells into blastocysts
or morulae
> Mouse ICSI
> Enucleation/nuclear transfer
> Embryo biopsies, e.g., for PGD
> DNA /RNA injection
www.eppendorf.com/cellmanipulation
Eppendorf ®, the Eppendorf logo and Eppendorf PiezoXpert ® are registered trademarks of Eppendorf AG, Germany. U.S. Design Patents are listed on www.eppendorf.com/ip
All rights reserved, including graphics and images. Copyright© 2016 by Eppendorf AG · The Eppendorf PiezoXpert® is designed for research use only.
I would lastly like to thank all of our sponsors and exhibitors. Your generous support
of has been vital for the success of this meeting, without which, this meeting would
not be possible.
I hope that this conference will not only demonstrate to our community the
developments in the field of transgenic technologies but also inspire and motivate all
participants in their projects and services.
Sincerely
Radislav Sedláček
Chair of the TT2016 Organising Committee
5
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
COMMITTEES & ORGANIZERS
Organizing Committee
Petr Bartunek
Inken M. Beck
Martina Crispo
Nicole Chambers
Kallayane Chawengsaksophak
Boris Jerchow
Zbynek Kozmik
Christian Mosimann
Ronald Naumann
Jan Parker-ornburg
Radislav Sedlacek – chair
Scientific Advisory Committee
Wojtek Auerbach
Jiri Forejt
Alexandra L. Joyner
Carlisle P. Landel
K. C. Kent Lloyd
Lluís Montoliu
Andras Nagy
Janet Rossant
Francis Stewart
Prague, Czech Republic
Prague, Czech Republic
Montevideo, Uruguay
Prague, Czech Republic
Prague, Czech Republic
Berlin, Germany
Prague, Czech Republic
Zürich, Switzerland
Dresden, Germany
ISTT, Houston, TX, USA
Prague, Czech Republic
Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA
Institute of Molecular Genetics, Prague, Czech Republic
Sloan-Kettering Institute, New York, NY, USA
Transposagen Biopharmaceuticals, Lexington, KY, USA
University of California, Davis, CA, USA
Centro Nacional de Biotecnología, Madrid, Spain
Mount Sinai Hospital, Lunenfeld-Tanenbaum
Research Institute, Toronto, Canada
University of Toronto, Canada
e Biotechnology Center (BIOTEC),
Technische Universität Dresden, Germany
Meeting Organizers
GUARANT International spol. s r.o.
Na Pankráci 17, 140 21 Prague 4, Czech Republic
Tel.: +420 284 001 444, Fax: +420 284 001 448
E-mail: tt2016@guarant.cz
Website: www.transtechsociety.org/tt2016
ISTT
Pat Arubaleze: administration@transtechsociety.org
6
The International Society for Transgenic Technologies (ISTT) seeks to foster
communication and technology sharing, to enhance scientific research, to
advance the field of animal transgenesis, particularly as it applies to useful
experimental models in biology, medicine and biotechnology, and to represent
the interests of an international body of professionals (scientists, technicians,
and graduate students) working in the field of transgenic technologies.
Become a member! Be part of our community!
The benefits of membership include priority access/reduced registration fees to
Transgenic Technology (TT) meetings, and free on-line access to Transgenic
Research, as well as unique online content (pictures, videos, methods, talks,
posters) through the members-only area of the ISTT website. Further
information on membership can be found at:
www.transtechsociety.org
ISTT Board of Directors:
President:
Jan Parker-Thornburg,
(USA)
Vice-President:
Benoît Kanzler,
(Germany)
Secretary & Treasurer:
Aimee Stablewski
(USA)
Officers: Boris Jerchow (Germany), Wojtek Auerbach (USA), Tom Fielder (USA),
Elizabeth Williams (Australia), Karen Brennan (Australia), Cheryl Bock (USA), Martina
Crispo (Uruguay) Members-elect: Branko Zevnik (Germany), Lynn Doglio (USA), Peter
Hohenstein (UK) Ex-officio board members: Lluís Montoliu (Spain, Past-President &
webmaster), Radislav Sedláček (CR,TT2016 Organizer), ISTT Administration: Pat
Arubaleze (USA)
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
Session 2: Generation of Transgenic Models II
(selected presentations)
Chair: Ralf Kühn
17.30–18.00
18.00–19.00
Meridian Hall
Welcome address
ISTT president and Organizing Committee
Opening Keynote Lecture
Andras Nagy
Utilising transposon-delivered transgenes for understanding
reprogramming
11.05–11.20
11.20–11.35
MONDAY
11.35–11.50
19.00
Come together evening
11.50–12.05
12.05–12.20
MONDAY, MARCH 21, 2016
08.00–18.00
Registration open
TUESDAY
Session 1: Generation of Transgenic Models I
Chair: Radislav Sedlacek
09.00–09.30
09.30–10.00
10.00–10.30
Meridian Hall
Charles A. Gersbach
Genome and Epigenome Editing with CRISPR/Cas9 for Gene erapy
and Disease Modeling
Ralf Kuhn
Direct production of mouse mutants using engineered nucleases
in one-cell embryos
Lluis Montoliu
CRISPR-ing the non-coding genome
12.20–13.30
Séverine Ménoret
Increased efficiency of BAC transgenesis by piggyBac transposition but
not by CRISPR/Cas9 targeted integration for the generation of human
SIRPalpha rats
Grzegorz Kreiner
Targeting nucleolus – a new approach in generating transgenic mouse
models of neurodegenerative diseases and their exploitation to study
possible neuroprotective
Melissa A. Larson
Nuclear Transfer between Strains of Inbred Mice
Christian Mosimann
Post-genome editing approaches for recombinase genetics in zebrafish
Andrei Golovko
10 Years of TIGM: Important Lessons and Future Perspectives
Anna Anagnostopoulos
Using the Human – Mouse: Disease Connection to Identify Mouse
Models of Human Disease
MONDAY
10.50–11.05
Opening Session
Chairs: Radislav Sedlacek & Jan Parker-ornburg
LUNCH
TUESDAY
SUNDAY
SUNDAY, MARCH 20, 2016
Meridian Hall
Lunch session (Sponsored lecture)
Meridian Hall
12.20–13.20 Merck, Sigma-Aldrich Chemie GmbH
Caroline Beckett (CRISPR Product Manager)
Designing the Next Phase in Transgenic Genome Editing: Advanced
CRISPR applications
WEDNESDAY
Lunch session (Sponsored lecture)
Tycho+Kepler Hall
12.20–13.20 Charles River
Jean Cozzi (Charles River); Guillaume Pavlovic (Phenomin ICS)
Designer Genes – providing the right fit
WEDNESDAY
SCIENTIFIC PROGRAM
SUNDAY
March 20–23, 2016, Prague, Czech Republic
10.30–10.50
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13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
15.00–15.15
Session 4: Beyond Transgenic Model Generation
Chair: Yann Herault
15.15–15.45
TUESDAY
15.45–16.15
16.15–16.45
Meridian Hall
16.45–17.00
WEDNESDAY
17.00–17.30
17.30–18.00
18.00–18.30
10
09.00–09.30
09.30–10.00
Meridian Hall
Francis Mojica
Taking a look at the CRISPR biology and applications from a historical
perspective
Bernd Zetsche
Cpf1 is a single-RNA-guided endonuclease of a Class 2 CRISPR-Cas
system
Konstantin Severinov
A pipeline approach for discovery of novel CRISPR-Cas systems
Meridian Hall
Eckhard Wolf
COST Action BM1308 “Sharing Advances on Large Animal Models –
SALAAM”
Yonglun Luo (Alun)
Efficient precision gene editing in pigs: Towards a new era in generating
genetically designed pigs of human diseases and regenerative medicine
Chris Proudfoot
Genome engineering livestock
10.00–10.15
Session 7: Advances in Animal Biotechnology II
(selected presentations)
Chair: Eckhard Wolf
10.30–10.45
10.45–11.00
11.00–11.15
SooYoung Yum
Production and Analysis of a multi-copy integrated transgenic cattle via
transposon
Amy Kaucher
Production of germline ablated male pigs via Crispr/Cas editing of the
NANOS2 gene
Sean Stevens
Pig Genome Engineering for Xenotransplantation
Wiebke Garrels
Multiplex Transgenesis in Cattle via the Sleeping Beauty Transposon
System
Session 8: Parallel Sessions – Animal Ethics
Chair: Boris Jerchow
11.20–11.40
11.40–12.00
12.00–12.20
Meridian Hall
SUNDAY
08.30–09.00
10.15–10.30
Mary Dickinson
Analysis of embryonic lethal mutations in mice using 3D imaging
Nicholas Gale
A large scale mouse phenotyping screen for the discovery of novel
biotech targets in oncology and angiogenesis and other therapeutic
areas
Kent Lloyd
An update on progress by the International Mouse Phenotyping
Consortium (IMPC)
Session 5: World of Nucleases: Discovery and Development
Chair: Lluis Montoliu
Session 6: Advances in Animal Biotechnology I
Chair: Bruce Whitelaw
MONDAY
MONDAY
Marina Gertsenstein
Associate Director of the Transgenic Core
e Centre for Phenogenomics (TCP)
Cord Brakebusch
Head of the Transgenic Mouse Core Facility;
Biotech Research and Innovation Centre (BRIC) at the University of
Copenhagen, Denmark
Lauryl Nutter
Mouse line production manager for IMPC projects
e Centre for Phenogenomics (TCP)
Ignacio Anegon
Director of the Transgenesis Rats ImmunoPhenomics (TRIP) Facility –
Nantes, France
TUESDAY, MARCH 22, 2016
TUESDAY
SUNDAY
13.30–15.00
Meridian Hall
Meridian Hall
Henriette Bout
Ethical aspects of the Crispr/Cas9 technology
Aurora Brønstad
e AALAS-FELASA Working Group on Harm-Benefit analysis of animal
studies
Michelle Stewart
e Four R’s of phenotyping GA mice – Robustness, Reproducibility,
Rigorousness, and Randomisation
WEDNESDAY
Session 3: Roundtable: Running a Transgenic Service Facility
Chair: Steve Murray
March 20–23, 2016, Prague, Czech Republic
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13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
12.00–12.20
12.20–14.00
LUNCH
MONDAY
Lunch session (Sponsored Lecture)
12.20–13.20
Applied StemCell, Inc.
Ruby Yanru Chen-Tsai
Genome engineering using TARGATTTM in rats, rabbits and pigs
Session 9: Gene Manipulation and Genome Editing
and (disease) Models I.
Chair: Jan Parker-ornburg
TUESDAY
14.00–14.30
14.30–15.00
WEDNESDAY
15.15–15.30
15.30–15.45
Meridian Hall
Valentino Gantz
e implications of active genetics
Didier Stainier
Genetic compensation induced by deleterious mutations but not gene
knockdowns
Session 10: Gene Manipulation and Genome Editing
and (disease) Models II. (selected presentations)
Chairs: Karen Brennan, Cheryl Bock
15.00–15.15
Meridian Hall
Meridian Hall
Yann Herault
Modelling and understanding rare genetic diseases with intellectual
disabilities for tomorrow’s treatment
Katharina Boroviak
e possibilities and limitations of CRISPR/Cas9 in mouse zygotes
Kevin A. Peterson
CRISPR/Cas9 mediated gene modification provides a robust platform
for modeling developmental disorders
16.00–16.45
Richard R. Behringer
Transgenic approaches in diverse animals species
16.45–18.00
ISTT General Assembly
19.15
Gala dinner
Meridian Hall
Meridian Hall
Žofín Palace
WEDNESDAY, MARCH 23, 2016
Session 11: Technology Development II
Chairs: Lynn Doglio, Martina Crispo
08.30–09.00
09.00–09.30
09.30–10.00
Meridian Hall
Masato Ohtsuka
GONAD and Easy (Isi)-CRISPR: novel mouse genome engineering tools
Haoyi Wang
CRISPR-Cas9 Application in Mouse Model Creation and Transcription
Regulation
Tomomi Aida
Gene cassette knock-in in mice with cloning-free CRISPR/Cas system
10.00–10.15
Session 12: Genetics, Epigenetics, Stem Cell Manipulation
Chair: Jiri Forejt
10.15–10.45
10.45–11.15
11.15–11.45
11.45–13.30
Meridian Hall
Robin Lovell-Badge
Regulation of Sox9 in the gonad during sex determination
F. Kent Hamra
Rat Germline Editing in Donor Spermatogonial Stem Cells
John Schimenti
GWIS: Genetics with Interrogation of SNPs
LUNCH & Poster Session
MONDAY
11.40–12.00
Toru Takeo
Efficient production of mouse oocytes using superovulation by
immunization against inhibin
Jeff Batton
New Cost-Effective Methods for Stem Cell Procedures on PreImplantation Embryos
Sandra Hope
Microfabricated Lance Array Nanoinjection system delivers CRISPRCas9 to Hundreds of ousands of Cells Simultaneously
Orbis pictus lecture I.
Chairs: Jan Parker-ornburg, Radislav Sedlacek
TUESDAY
SUNDAY
11.20–11.40
Tycho+Kepler Hall
WEDNESDAY
Session 8: Parallel Sessions – Technology Development I
Chair: Ronald Naumann
March 20–23, 2016, Prague, Czech Republic
SUNDAY
March 20–23, 2016, Prague, Czech Republic
15.45–16.00
12
13
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
14.00–14.15
MONDAY
14.15–14.30
17.10–17.55
17.55–18.15
18.15
14.45–15.30
Denis Duboule
A Genetic Approach of Long-range Gene Regulation During
Development and Evolution
Closing Remarks
Presentation of ISTT Registration Award Diplomas
e ISTT Best Poster Awards, sponsored by Charles River Laboratoris
International, Inc.
Presentation of the TT2017 Meeting
Susan Tamowski, e University of Utah, Salt Lake City, Utah
TUESDAY
16.00–16.30
Meridian Hall
Rene Maehr
Towards using pluripotent stem cell-based disease models to study
immune syndromes
Ron Weiss
Mammalian Synthetic Biology: From Parts to Modules to erapeutic Systems
WEDNESDAY
16.30–16.45
Session 15: ISTT Young Investigator Award
Meridian Hall
and Best Poster Awards
Chair: omas Zeyda, Director of Technology Integration, Ingenious Targeting Laboratory
16.45–16.50
Close of TT2016 Meeting
Meridian Hall
omas Boehm
Genetic basis of lymphoid organ formation
Session 14: Genetics & Models Pluripotency/iPS cells
Chairs: Zbynek Kozmik, John Schimenti
15.30–16.00
Meridian Hall
SUNDAY
Session 16: Closing Keynote Lecture
Chairs: Radislav Sedlacek, Benoît Kanzler
14.30–14.45
Orbis pictus lecture II.
Chairs: Benoît Kanzler, Radislav Sedlacek
14
Pablo Ross
Embryonic stem cells and interspecies blastocyst complementation in
farm animals
TUESDAY
13.45–14.00
Marie-Christine Birling
Generation of genomic structural variants by CRISPR/Cas9 genome
editing in rodents
Petr Kasparek
TALEN-mediated inactivation of Klk5 and Klk7 rescues lethal phenotype
of Netherton syndrome mouse model
Javier Martín-González
A powerful new tool to improve immune-compromised mouse models:
derivation of NRG embryonic stem cell lines.
Prem Premsrirut
RNAi and CRISPR/Cas9 based In Vivo Models for Drug Discovery
16.50–17.10
WEDNESDAY
SUNDAY
13.30–13.45
Meridian Hall
MONDAY
Session 13: Generation of Transgenic Models III:
Models of Diseases and Applications (selected presentations)
Chairs: Elizabeth Williams, Aimee Stablewski
March 20–23, 2016, Prague, Czech Republic
Presentation of the 4th ISTT Young Investigator Award
Sponsored by
15
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
March 20–23, 2016, Prague, Czech Republic
POSTERS
Posters will be mounted in the ZENIT room and will be available for viewing from 19:00
Sunday, March 20, 2016. e posters will remain on display for the duration of the
meeting and can be viewed until the specified times:
Monday, March 21, 2016
Tuesday, March 22, 2016
Wednesday, March 23, 2016
20:00
18:00
17:00
During the poster session (Wednesday, March 23, 2016; 11:45–13:30) the authors of
the posters will be present and available for more in-depth discussion.
List of Posters
P-001 New prospects for genetic analysis and characterization of inbred mice (ID 7)
Peter Dobrowolski1, Olaf Gelsen1, Melina Fischer2, Ronald Naumann3
1
GVG Genetic Monitoring GmbH, Leipzig, Germany; 2Genolytic GmbH, Leipzig, Germany; 3Max Planck Institute of
Molecular Cell Biology and Genetics, Dresden, Germany
P-002 e neglected Y-chromosome of inbred mice (ID 27)
Peter Dobrowolski1, Olaf Gelsen1, Melina Fischer2
1
GVG Genetic Monitoring GmbH, Leipzig, 2Genolytic GmbH, Leipzig
P-003 Applying Digital Droplet PCR (ddPCR) to screen for aneuploidy and gene
targeting events in embryonic stem cells in a high-throughput pipeline (ID 33)
Gemma F. Codner1, Loic Lindner2, Adam Caulder1, Marie Wattenhofer-Donzé2, Adam
Radage1, Annelyse Mertz2, Benjamin Eisenmann2, Joffrey Mianne 2, Edward P. Evans1,
Colin V. Beechey1, Marie-Christine Birling2, Yann Herault2, Guillaume Pavlovic2,
Lydia Teboul1
1
2
e Mary Lyon Centre, Medical Research Council, Harwell Science and Innovation Campus, Oxon, UK;
PHENOMIN Institut Clinique de la Souris, ICS; GIE CERBM, CNRS, INSERM, University of Strasbourg, France
P-004 Improvement of GONAD (genome-editing via oviductal nucleic acids
delivery), one of the zygote injection-free genome editing systems targeted to
preimplantation embryos (ID 36)
Masahiro Sato1, Channabasavaiah B. Gurumurthy2, Satoshi Watanabe3, Masato Ohtsuka4
1
Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Japan; 2Mouse
Genome Engineering Core Facility, Department of Genetics, Cell Biology and Anatomy, University of Nebraska
Medical Center, Omaha, NE, USA; 3Animal Genome Research Unit, Division of Animal Science, National Institute
of Agrobiological Sciences, Ibaraki, Japan; 4Division of Basic Molecular Science and Molecular Medicine, School
of Medicine, Tokai University, Kanagawa, Japan
P-005 Generation of genome-modified mice by various procedures of CRISPR/
Cas9 systém (ID 38)
Karolina Piotrowska-Nitsche1, Teresa Quackenbush1, Yao Huang1, Tamara Caspary1,2
1
16
2
Transgenic Mouse/Gene Targeting Core Facility, Emory University School of Medicine, Atlanta, USA;
Department of Human Genetics, Emory University School of Medicine, Atlanta, USA
P-006 Improving the injection procedure for manipulating the chicken embryo
(ID 41)
Shahin Eghbalsaied, Ahmadreza Rahaee, Saeedeh Moghaddasi, Zohreh Hajihoseini
Transgenesis Center of Excellence, Isfahan branch, Islamic Azad University, Isfahan, Iran
P-007 Heritable gene disruption in goats with CRISPR/Cas9 results in expected
phenotypes (ID 45)
Xiaolong Wang1, Yiyuan Niu1, Bei Cai1, Baohua Ma2, Xingxu Huang3, Lei Qu4, Yulin Chen1*
1
College of Animal Science and Technology, Northwest A&F University, Yangling, China; 2College of Veterinary
Medicine, Northwest A&F University, Yangling, China; 3School of Life Science and Technology, ShanghaiTech
University, Shanghai, China; 4Yulin University, Life Science Research Center, Yulin, China
P-008 Mice double-deficient for Klk5 and Klk7 generated by programmable
nucleases show altered epidermal barrier (ID 54)
Zuzana Ileninova, Petr Kasparek, Henrieta Palesova, Ivan Kanchev, Radislav Sedlacek
Czech Centre for Phenogenomics, Institute of Molecular Genetics AS CR, v. v. i., Prague, Czech Republic
P-009 Simplification of embryo vitrification using cryovial adapters (ID 63)
K.John McLaughlin1, N. Adrian Leu2, Benoit Kanzler3, Erin Grove1
1
Center for Molecular and Human Genetics, Nationwide Children‘s Hospital, Ohio State University, Columbus,
Ohio, USA; 2Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania, USA; 3MaxPlanck-Institut f. Immunbiologie u. Epigenetik, Freiburg, Germany
P-010 Site-specific recombination in pre-implantation stage embryos and adult
reproductive tissues using cell-permeable Cre protein (ID 71)
Marina Gertsenstein1, Lauryl M.J. Nutter1,2, Steffen Biechele3, Andrea Jurisicova4,
Janet Rossant5
1
e Centre for Phenogenomics (TCP), Toronto, Canada; 2Physiology & Experimental Medicine, e Hospital
for Sick Children, Toronto, Canada; 3Center for Reproductive Sciences, Department of Obstetrics, Gynecology
and Reproductive Sciences; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,
University of California, San Francisco, USA; 4Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital,
Toronto, Canada; Department of Physiology; Department of Obstetrics and Gynecology, University of Toronto,
Canada; 5Program in Developmental and Stem Cell Biology, Peter Gilgan Centre for Research and Learning, e
Hospital for Sick Children, Toronto, Canada; Department of Molecular Genetics, University of Toronto, Canada
P-011 e use of heteroduplex mobility assay for screening genome-edited
animals (ID 104)
Vanessa Chenouard, Lucas Brusselle, Séverine Remy, Séverine Ménoret, Claire Usal,
Laure-Hélène Ouisse, Ignacio Anegon and Laurent Tesson
INSERM UMR 1064-ITUN, CHU de Nantes, France; Platform Transgenic Rats and ImmunoPhenomics; Nantes, France
P-012 A vector with a single promoter for in vitro transcription and mammalian
cell expression of CRISPR gRNAs (ID 131)
Peter J. Romanienko1, Joseph Giacalone, Joanne Ingenito, Yijie Wang, Mayumi Isaka,
omas Johnson, Yun You1 and Willie H. Mark1
Mouse Genetics Core Facility, 1Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan
Kettering Cancer Center, New York, USA
17
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
P-013 Guidelines for generation of genetically modified mice via CRISPR/Cas
(ID 145)
Yueh-Chiang Hu, Yinhuai Chen, Huirong Xie, Alexandra Falcone, Susan R. Martin,
Melissa A. Scott
March 20–23, 2016, Prague, Czech Republic
P-020 Effect of Leptin on Derivation Rate of Mouse Embryonic Stem (ES) Cell
Line (ID 98)
Ali Cihan Taskin1, Ahmet Kocabay1, Tevfik Tamer Onder2, Ayyub Ebrahimi2
1
Koc University, College of Sciences, Istanbul, Turkey; 2Koc University, College of Medicine, Istanbul, Turkey
Cincinnati Children‘s Hospital Medical Center, Cincinnati, USA
P-014 Testing the CARD ultra-superovulation IASe protocol for EMMA archiving
purposes (ID 150)
Julia Fernández1,2, María Jesús del Hierro1, Marta Castrillo1, Isabel Martín-Dorado1, Toru
Takeo3, Naomi Nakagata3 and Lluís Montoliu1,2
1
Spanish EMMA node at the National Centre for Biotechnology (CNB-CSIC) and 2CIBERER-ISCIII, Madrid, Spain;
3
CARD-University of Kumamoto, Japan
P-015 Development of integrase-based TARGATTTM method for generating sitespecific transgenic rat models (ID 167)
Qi Zheng, Lin Yan, Jimmy Tai, Lingjie Kong, Jinling Li and Ruby Yanru Chen-Tsai
P-021 Development of an iPS cell-based model to study the role of p73 gene in
adipocyte differentiation (ID 133)
Laura Maeso-Alonso1, Marta Martin-Lopez1, Sandra Fuertes-Alvarez1,
Margarita M. Marques2 and Maria C. Marin1
1
Department of Molecular Biology and Institute of Biomedicine, University of Leon, Spain; 2Department of
Animal Production and Institute for Animal Breeding and Health, University of Leon, Spain
P-022 Investigation the role of microRNAs in early embryonic development and
stem cells in rabbit and chicken (ID 147)
Mahek Anand1,2, Pouhneh Maraghechi1, Kinga Nemeth1, Bence Lazar1, Elen Gocza1
Applied StemCell, Inc., USA
Department of Animal Husbandry Sciences, Szent Istvan University, Godollo, Budapest, Hungary; 2National
Agricultural Research and Innovation Center -Agricultural Biotechnology Center, Godollo, Budapest, Hungary
P-016 Comparation of three different cell transfection reagents in ram
spermatozoa transfection to obtain transgenic sheep embryos (ID 172)
Gül Bakirer Oztürk1, Mehmet Koray GöK2, Kamber Demir3, Ramazan Arici3, Ayşe Can3,
Saadet K.Pabuccuoğlu2, Sema Birler3,Serhat Pabuccuoğlu2.
P-023 Generation of Induced Pluripotent Stem Cells from Human Umbilical Cord
Vein Mesenchymal Stem Cells (ID 185)
Fardin Fathi, Mehdad Abdi, Sonia Zare, Seyed hadi Anjamrooz,
Mohammad Jafar Rezaei, Jalal Rostamzadeh
1
Department of Laboratory Animal Science, Institution of Experimental Medicine, Istanbul University,
Turkey; 2Department of Chemical Technologies, Faculity of Chemical Engineering, Istanbul University, Turkey;
3
Department of Reproduction and Artificial Insemination, Faculity of Veterinary Medicine, Istanbul University,
Turkey
Cellular and Molecular Research Center, Kurdistan University of Medical Science, Sanandaj, Iran
P-017 Exclusive germline transmission through mouse chimeras from sterile
male blastocysts (ID 173)
John Dixon1, Frank Koentgen1, Jiangwei Lin2, Markella Katidou2, Isabelle Chang2, Mona
Khan2, Jacqui Watts1 & Peter Mombaerts2*
1
Ozgene Pty Ltd, Bentley, WA, Australia; 2Max Planck Research Unit for Neurogenetics, Frankfurt, Germany
P-018 Embryonic stem cell culture conditions support distinct states associated
with different developmental stages and potency (ID 57)
Javier Martín-González1, Sophie M. Morgani2, Kasper Bonderup1, Sahar Abelchian1,
Cord Brakebusch1 and Joshua M. Brickman2
Transgenic Core Facility, University of Copenhagen, Denmark; 2e Danish Stem Cell Centre - DanStem,
University of Copenhagen, Denmark
1
P-024 A rapid and efficient one-step approach to generate sgRNA template for
producing genetic modified mice (ID 6)
I-Shing Yu1,2, Sheng-Kai Chang1,3, Zu-Shan Huang1, Feng-Lin Pu1, Po-Yueh Wu1,
Huang-Yi Ling1, Sheng-Wen Wang1, Wei-Jou Lin1, Cheng-Ju Wang1, Wei-Nien Chen1,
Chien-Hui Wu1, and Shu-Wha Lin1,2,3
1
Transgenic Mouse Models Core Facility, Ministry of Science and Technology, Taiwan; 2Laboratory Animal Center,
College of Medicine, National Taiwan University, Taipei, Taiwan; 3Department of Clinical Laboratory Sciences and
Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan
P-025 Designing for success: the right CRISPR design strategies for the right
experiment (ID 8)
Leigh Brody1, Victor Dillard1, Neil Humphryes1, Riley Doyle1
1
Desktop Genetics, London, UK
1
P-019 Developing a universal gene targeting construct to introduce complex
modifications via co-electroporation of CRISPR reagents into mouse ESCs (ID 60)
Alasdair J. Allan, Joffrey Q. Mianne, Jorik Loeffler, Marina Maritati, Gemma F. Codner,
Lydia Teboul
e Mary Lyon Centre, MRC Harwell, HSIC, Oxon, UK
18
P-026 Developing transgenic tools to study Notch signaling in intact neural stem
cell niches of the adult vertebrate brain in vivo (ID 12)
Sara Ortica Gatti, Nicolas Dray, Sébastien Bedu, Isabelle Foucher, Laure Bally-Cuif
DevEvo Department, Paris-Saclay Institute for Neuroscience (Neuro-PSI), Gif-sur-Yvette, France
P-027 Promoting the efficiency of CRISPR/Cas9-mediated knock-in mice through
homology-directed repair via poly(A) tailing and Scr7 treatment (ID 19)
Tzu-Yu Chou1, Pi-Fang Tsai1, Yi-Fang Tu2 and Ching-Yen Tsai1
1
Transgenic Core Facility, Inst. of Molecular Biology, Academia Sinica, Taipei, Taiwan; 2 Department of Pediatrics,
National Chang Kung University Hospital, College of Medicine, National Chang Kung University, Tainan, Taiwan
19
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
P-028 Selecting guide RNAs with high on-target and low off-target activity using
CRISPOR (ID 20)
Maximilian Haeussler1, Kai Schönig2, Hélène Eckert3, Alexis Eschstruth4,
Sylvie Schneider-Maunoury4, Alena Shkumatava3, Jim Kent1, Jean-Stephane Joly5,
Jean-Paul Concordet6
P-034 Efficient CRISPR/Cas9 genome engineering using zygotes and embryos
derived from transgenic mice overexpressing Cas9 (ID 51)
Alberto Cebrian-Serrano, Chris Preece, Daniel Biggs and Benjamin Davies
1
Santa Cruz Genomics Institute, MS CBSE, University of California, Santa Cruz CA, USA; 2Central Institute of
Mental Health, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany; 3Institut Curie, CNRS
UMR3215, INSERM U934, Paris, France; 4CNRS UMR 7622, INSERM U1156, Sorbonne Université Paris 06, Paris,
France; 5TEFOR Infrastructure, Gif-sur-Yvette, France; 6INSERM U1154, CNRS UMR 7196, Muséum National
d‘Histoire Naturelle, Paris, France
P-035 e questions you should ask yourself for efficient CRISPR/Cas9 genome
editing (ID 56)
Guillaume Pavlovic1, Pascale Mercier3, Valérie Risson4, Philippe Schmitt5,
Marie-Christine Birling1, Francina Langa6, Suzy Markossian4, Lydia Teboul7,
Erwana Harscoet8, Karelia Lipson9, Sandra Offner10, Fabien Angelis11, Isabelle Barde10,
Matthieu Bringart12, Jean-Paul Concordet13, Frédéric Fiore11, Térésa Jagla14,
Laurent Tesson15, Jean-Stéphane Joly16 and Yann Hérault1,2
P-029 Generation of inducible cas9 knock in mouse embryonic stem cell and
transgenic mouse for in vivo gene targeting (ID 26)
Kyung-Jun Uh1, Yeong-Hee Jeong1, Woo-Sung Hwang1, Ji-Hyun Bae1, Da-Eun Jang2,
In-Yeong Choi1, Su-Cheong Yeom2
1
Designed Animal and Transplantation Research Institute, Greenbio Research and Technology, Seoul National
University, Daewha, Pyeongchang, Kangwon, Korea; 2Graduate School of International Agricultural Technology,
Seoul National University, Daewha, Pyeongchang, Kangwon, Korea
P-030 Optimisation and high-throughput production of CRISPR/Cas9-mediated
knockout mouse strains (ID 30)
Ed Ryder, Brendan Doe, Barry Rosen, Joanna Bottomley, Graham Duddy, Mark omas,
Ellen Brown, Diane Gleeson, Dominique Von Schiller, Debarati Sethi, Michael Woods,
Sanger Mouse Genetics Project, Ramiro Ramirez-Solis
Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
P-031 In vivo correction of an infertility disease by CRISPR-Cas9-mediated
genome editing of the adult mouse germline (ID 31)
Manuel Sánchez-Martín1, Ignacio García-Tuñón2, Natalia Felipe2, Laura Gomez-H2, Hiroki
Shibuya4, Jose Luis Barbero6, John C. Schimenti7, Guillermo Montoya5, Yoshi Watanabe4,
Elena Llano3, Alberto M. Pendas2
1
Department of Medicine and Transgenic Facility, University of Salamanca, Spain; 2Instituto de Biología
Molecular y Celular del Cáncer (CSIC-USAL), Salamanca, Spain; 3Departamento de Fisiología y Farmacología,
Universidad de Salamanca, Spain; 4Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular
Biosciences, University of Tokyo, Japan; 6Centro de Investigaciones Biológicas (CSIC), Madrid, Spain; 7Center for
Vertebrate Genomics, Cornell University, Ithaca, USA
P-032 Generation of new germline mutations in mouse thyroid hormone receptor
TRα1 by in ovo CRISPR/Cas9 genome editing to model the RTHα genetic disease (ID 34)
Suzy Markossian1, Valérie Risson2, Marie Teixeira2, Frédéric Flamant1
1
Ecole Normale Supérieure de Lyon, IGFL, INRA USC 1370, CNRS UMR5242, France; 2Plateau de Biologie
Expérimentale de la Souris, Ecole Normale Supérieure de Lyon, SFR BioSciences Gerland, France
P-033 A fast, easy and reliable method for the generation of KO mice using
CRISPR/Cas9 by a single person: a small laboratory perspective (ID 40)
Fabien Delerue, Lars Ittner
Transgenic Animal Unit (TAU), Mark Wainwright Analytical Centre, University of New South Wales (UNSW),
Sydney, Australia
20
March 20–23, 2016, Prague, Czech Republic
Wellcome Trust Centre for Human Genetics, University of Oxford, UK
1
Institut Clinique de la Souris, PHENOMIN, CNRS UMR7104, INSERM U964, Université de Strasbourg, Illkirch,
France; 2Institut de Genetique Biologie Moleculaire et Cellulaire (IGBMC), CNRS, INSERM, Université de
Strasbourg, UMR7104, UMR964, Illkirch, France; 3Centre National de la Recherche Scientifique, Institut de
Pharmacologie et de Biologie Structurale, Toulouse, France; 4Institut de Génomique Fonctionnelle de Lyon,
Université de Lyon, Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche
Agronomique, École Normale Supérieure de Lyon, France; 5CELPHEDIA, CNRS, Illkirch, France; 6Centre
d‘Ingénierie Génétique Murine, Institut Pasteur, Paris, France; 7e Mary Lyon Centre, Medical Research Council,
Harwell, Oxfordshire, UK; 8INRA UMR1198, Biologie du Développement et Reproduction, Jouy en Josas, France
; 9SEAT, Villejuif; 10School of Life Sciences and Frontiers in Genetics Program, Ecole Polytechnique Fédérale
de Lausanne (EPFL), Switzerland; 11Centre d’Immunophénomique (CIPHE), Aix Marseille Université, Marseille,
France; 12INSERM-US006 CREFRE (Centre Régional d‘Exploration fonctionnelle et Ressources Expérimentales)
Toulouse; 13TACGene, Muséum National d‘Histoire Naturelle, CNRS UMR 7196, INSERM U565, Paris, France;
14
Genetics, Reproduction and Development, Institut National de la Santé et de la Recherche Médicale U931,
Centre National de la Recherche Scientifique UMR6247, Clermont University, Clermont-Ferrand, France;15INSERM
UMR 1064-ITUN, Centre Hospitalier Universitaire de Nantes, Faculté de Médecine, France; 16TEFOR, Centre
National de la Recherche Scientifique, Gif-sur-Yvette, France
P-036 Enhance genome editing in mammalian cells with a modified dualfluorescent surrogate systém (ID 58)
Yan Zhou1, Yong Liu1, Dianna Hussmann1, Peter Brøgger1,
Rasha Abdelkadhem Al-Saaidi2, Shuang Tan1, 3, Lin Lin1, Trine Skov Petersen1,
Guang Qian Zhou3, Peter Bross2, Lars Aagaard1, Tino Klein5, Sif Groth Rønn6,
Henrik Duelund Pedersen6, Lars Bolund1, 4, 7, Anders Lade Nielsen1,
Charlotte Brandt Sørensen2, Yonglun Luo1, 6, 7, *
1
Department of Biomedicine, Aarhus University, Denmark ; 2Department of Clinical Medicine, Aarhus University,
Denmark; 3Shenzhen Key Laboratory for Anti-aging and Regenerative Medicine, Health Science Center,
Shenzhen University, China; 4BGI-Shenzhen, China; 5Department of Histology, Gubra A/S., Hørsholm, Denmark;
6
Department of GLP and Obesity Pharmacology, Novo Nordisk A/S., Måløv, Denmark; 7e Danish Regenerative
Engineering Alliance for Medicine (DREAM), Aarhus University, Denmark.
*Corresponding author: Yonglun Luo (ALUN@BIOMED.AU.DK)
P-037 Engineering the mouse genome using CRISPR/Cas9 technology (ID 59)
Joffrey Mianné, Adam Caulder, Gemma Codner, Ruairidh King, Rachel Fell,
Marina Maritati, Alasdair Allan, James Jarrold, Martin Fray, Wendy Gardiner,
MLC Microinjection team, Sara Wells and Lydia Teboul
e Mary Lyon Centre, MRC Harwell, Didcot, UK
21
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
P-038 Improved CRISPR-mediated genome editing specificity with destabilized
Cas9 (PEST-Cas9) (ID 61)
Yong Liu1, Yan Zhou1, Trine Skov Petersen1, Lin Lin1, Lars Bolund1, Yonglun Luo1*
1
Department of Biomedicine, Aarhus University, Aarhus, Denmark (*Corresponding author: alun@biomed.au.dk)
P-039 Characterisation of complex CRISPR induced genotypes in mice (ID 64)
Ruairidh King, Joffrey Mianné, Adam Caulder, Gemma Codner, Rachel Fell,
Marina Maritati, Alasdair Allan, James Jarrold, Martin Fray, Wendy Gardiner,
MLC Microinjection team, Sara Wells and Lydia Teboul
e Mary Lyon Centre, MRC Harwell, Harwell Campus, Oxfordshire, UK
P-040 Efficient generation of mouse models with the CRISPR/Cas9 technology
(ID 73)
Karelia Lipson Ruffert1, Jean-Paul Moussu1, Miguel Taillepierre1, Céline Becker1,
Catherine Cailleau1, Karine Charton2,3, Laurence Suel2,3, Sara F Henriques2,
Matteo Bovolenta4, Isabelle Richard2,3 and Jean-Pierre de Villartay5,6
1
SEAT-TAAM PHENOMIN, UPS44 CNRS, Villejuif, France; 2INSERM, U951, Evry, France; 3Généthon, R&D
department, INTEGRARE Research Unit, Evry, France; 4Department of Life Sciences and Biotechnology,
University of Ferrara, Ferrara, Italy; 5Laboratory of Genome Dynamics in the Immune System (DGSI), INSERM
UMR1163, Paris, France; 6Paris-Descartes, Sorbonne Paris Cité University, Imagine Institute, Paris, France
P-041 Cas9 RNA-guided nuclease – Efficient model generation in several strain
backgrounds (ID 74)
Lauryl M.J. Nutter1,2, Marina Gertsenstein1, Janet Rossant3
1
e Centre for Phenogenomics (TCP), Toronto, Canada; 2Physiology & Experimental Medicine, e Hospital for
Sick Children, Toronto, Canada; 3Developmental and Stem Cell Biology, Peter Gilgan Centre for Research and
Learning, e Hospital for Sick Children, Toronto, Canada and Department of Molecular Genetics, University of
Toronto, Canada
P-042 Validation of guide RNAs for CRISPR-Cas9 system using in-vitro cultured
mouse embryos (ID 76)
Jinping Luo1, Timothy Dahlem2, Susan Tamowski1
1
Transgenic and Gene Targeting Core, University of Utah, Salt Lake City, Utah, USA; 2Mutation Generation and
Detection Core, University of Utah, Salt Lake City, Utah, USA
P-043 Efficiently generates CRISPR/Cas9 knock-in and conditional mice using
in vitro one cell–controlled method (ID 77)
Sang Yong Kim1, Ping Zhou1, Amy Sun2, Crequer Amandine2, Marcus Hines2,
Adrian Erlebacher2, Sergei Koralov2 and David Levy3
1
Rodent Genetic Engineering Core, New York University, School of Medicine, Department of Pathology, USA;
New York University, School of Medicine, Department of Pathology, USA; 3New York University, School of
Medicine, Department of Pathology and Molecular Pathology, USA
2
P-044 CRISPR/Cas9 Genome Editing Pipeline for Mice and Rats (ID 81)
omas Saunders1,2, Wanda Filipiak1, Galina Gavrilina1, Anna LaForest1, Corey Ziebell1,
Michael Zeidler1, Elizabeth Hughes1
March 20–23, 2016, Prague, Czech Republic
P-045 High-throughput knockout mouse production using Cas9 RNA-guided
nuclease (ID 82)
Lauryl MJ Nutter1,2, Shinya Ayabe3, Gemma Codner4, Brendan Doe5, Graham Duddy5,
Angelina Gaspero6, Leslie Goodwin7, Jason Heaney6, Susan Kales7, Denise Lanza6,
Kent KC Lloyd8, Joffrey Mianne4, Stephen A Murray7, Yuichi Obata3, Kevin A Peterson7,
Ramiro Ramirez-Solis5, Ed Ryder5, John Seavitt6, Lydia Teboul4, Sara Wells4,
Brandon Willis8, Atsushi Yoshiki3
1,2
Physiology & Experimental Medicine, e Hospital for Sick Children, and 2e Centre for Phenogenomics,
Toronto, Ontario, Canada; 3RIKEN BioResource Center, Ibaraki, Japan; 4Mary Lyon Centre, MRC Harwell,
Oxfordshire, UK; 5e Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK; 6Department of Molecular and
Human Genetics, Baylor College of Medicine, Houston, USA; 7e Jackson Laboratory, Bar Harbor, USA; 8Mouse
Biology Program, UC Davis, Davis, USA
P-046 Rapid generation of knockout mice by pronuclear injection of a circular
plasmid expressing Cas9 and a single guided RNA (ID 83)
Cecilia Tomni, Katrin Schorr, Christian Hübner, Ingo Kurth
Jena University Hospital, Institute of Human Genetics, Jena, Germany
P-047 Dissection of genetic redundancy by the CRISPR/CAS system reveals
a novel role for FGF signaling during mouse eye development (ID 86)
Yas Furuta1,2, Takaya Abe1, Yui Yamashita1,2, Yoshiko Mukumoto1, Atsumi Denda1,
Mari Kaneko1,2, Megumi Watase1, Hiroshi Kiyonari1,2
1
Genetic Engineering Team and 2Animal Resource Development Unit, RIKEN Center for Life Science Technologies,
Kobe, Japan
P-048 iMITS: Supporting High-roughput Strain Production by CRISPR-based
Methods for the International Mouse Phenotyping Consortium (ID 87)
Peter Matthews and Terrence Meehan on behalf of the MPI2 Consortium
European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus,
Hinxton, Cambridge, UK
P-049 Generation of knockdown mice by CRISPR/Cas9-based targeted insertion
of artificial miRNA sequence (ID 88)
Hiromi Miura1,2, Channabasavaiah B Gurumurthy3, Rolen Quadros4, Masahiro Sato5,
Masato Ohtsuka1
1
Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai
University School of Medicine, Isehara, Kanagawa, Japan; 2Department of Regenerative Medicine, Basic
Medical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan; 3Developmental Neuroscience,
Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, USA;
4
Mouse Genome Engineering Core Facility, University of Nebraska Medical Center, Omaha, USA; 5Section of Gene
Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, Japan
P-050 Inquiring the genome: the use of CRISPR-Cas technique in a transient
transgenic strategy to interrogate regulatory element function (ID 89)
I. Rollán, M. Gómez, T. Rayón, C. Badía and M. Manzanares
Department of Functional Genomics, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain
1
Biomedical Research Core Facilities, Transgenic Animal Model Core; 2Department of Internal Medicine, Division
of Molecular Medicine and Genetics, University of Michigan Medical School, Ann Arbor, USA
22
23
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
March 20–23, 2016, Prague, Czech Republic
P-051 Generation of Gal-KO bovine fibroblast colonies using CRISPR/Cas9
technology and magnetic beads selection (ID 94)
Andrea Perota1, Irina Lagutina1, Corinne Quadalti1, Roberto Duchi1, Paola Turini1,
Gabriella Crotti1, Silvia Colleoni1, Giovanna Lazzari1,3, Cesare Galli1,2,3
P-058 CRISPRs system improves the overall efficiency in small transgenic
facilities (ID 110)
Maria Noel Meikle1, Geraldine Schlapp1, Ana Paula Mulet1, Adrián Capoano2,
Adriana Geisinger2, Martina Crispo1.
1
Avantea, Laboratorio di Tecnologie della Riproduzione, Cremona, Italy; 2Dept. of Veterinary Medical Science,
University of Bologna, Ozzano Emilia, Italy; 3Avantea Foundation, Cremona, Italy
1
Transgenic and Experimental Animal Unit, Institut Pasteur de Montevideo; 2Molecular Biology Unit, Instituto de
Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay
P-052 chNHE1 and chicken resistance to ALV-J: prospects for biotechnological
solution (ID 95)
Anna Lounkova, Jiri Plachy, Dana Kucerova, Marketa Reinisova, Jiri Hejnar
P-059 An attP Landing Pad in Rat Rosa26 Locus Allows Site-Specific IntegraseMediated Transgenesis (ID 115)
Shuqin Zhang1, Suxia Bai2, Xianling Zhao1, Xin Su1, Shumei Zhao1, Xiaohao Yao1, Nelson
Spruston1 and Caiying Guo1
Laboratory of Viral and Cellular Genetics, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic
1
P-053 Constitutively expressing hCas9 transgenic mouse generated through
targeting in Rosa26 locus (ID 96)
Irena Jenickova1, Maja Sabol2, Bjoern Schuster1, Petr Kasparek1, Inken Beck1,
Radislav Sedlacek1
1
Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, BIOCEV, Institute of
Molecular Genetics of the ASCR, v.v.i., Prague, Czech Republic; 2Laboratory for Hereditary Cancer, Division of
Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
P-054 Generation of transgenic and CRISPR/Cas9 KO/KI rat and mouse models
at the UAT (ID 99)
Ana Arbós, Sandra Turón, Miquel Garcia, Fàtima Bosch, Anna Pujol
Transgenic Animal Unit (UAT), Center for Animal Biotechnology and Gene erapy Center (CBATEG), Universitat
Autònoma de Barcelona (UAB), Spain
P-055 Gene edited rats by delivery of Cas9 protein using electroporation (ID 103)
Séverine Remy1,2, Vanessa Quillaud-Chenouard1,2, Laurent Tesson1,2,
Séverine Ménoret1,2, Claire Usal1,2, Lucas Brusselle1,2, Tuan Huan Nguyen1,3,
Anne De Cian 4, Carine Giovannangeli4, Jean-Paul Concordet4, and Ignacio Anegon1,2
1
INSERM UMR 1064-ITUN, CHU de Nantes, France; 2Platform Transgenic Rats and ImmunoPhenomics; Nantes,
France; 3Platform GenoCellEdit Nantes, France; 4INSERM U565, CNRS UMR7196, Museum National d’Histoire
Naturelle, Paris, France
P-056 Production of CRISPR/Cas9 generated mutants using frozen/thawed 1
cell C57Bl6/N mouse zygotes (ID 105)
Evelyn Grau, Ellen Brown, Mike Woods, Stuart Newman, Ed Ryder, Diane Gleeson,
Hannah Wardle-Jones, Graham Duddy, Ramiro Ramirez-Solis, Sanger MGP,
Katharina Boroviak and Brendan Doe
Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire, UK
P-057 2016 Update: Gene Editing Rat Resource Center (GERRC) (ID 109)
Jason Klotz2, Rebecca Schilling1,2, Michael Grzybowski1,2, Angela Lemke1,2,
Jocelyn Miller1,2, Anne Temple1,2, Allison Zappa1,2, Chieh-Ti Kuo1,2, Lynn Lazcares1,2,
Shawn Kalloway1,3, Jamie Foeckler1,3, Akiko Takizawa1,2, Jozef Lazar1, Howard J Jacob1,2,
Aron M Geurts1,2, Melinda Dwinell1,2
24
1
Genome Editing Rat Resource Center (GERRC), Human & Molecular Genetics Center; 2Department of Physiology,
Medical College of Wisconsin, Milwaukee, USA; 3Blood Center of Wisconsin, Blood Research Institute, Milwaukee, USA
Janelia Research Campus, HHMI, Ashburn VA, USA; 2Yale Genome Editing Center, Yale School of Medicine, New
Haven, CT, USA
P-060 Targeted Transgenesis by Nucleases: the experience of the Mouse
Genetics Engineering Center facility (ID 117)
Francina Langa, Gaëlle Chauveau-Le Friec, Ilta Lafosse, Carine Moigneu
Mouse Genetics Engineering Center, Institut Pasteur, Paris, France
P-061 Generation of targeted overexpressing models by CRISPR/Cas9 and need
of careful validation of your knock-in line obtained by nuclease genome editing
(ID 119)
Guillaume Pavlovic1, Valérie Erbs1, Philippe André1, Jacquot Sylvie1, Benjamin Eisenman1,
Dominique Dreyer1, Romain Lorentz1, Marie Wattenhofer-Donze1,
Marie-Christine Birling1, Yann Hérault1,2
1
Institut Clinique de la Souris, PHENOMIN, CNRS UMR7104, INSERM U964, Université de Strasbourg, Illkirch,
France; 2Institut de Génétique Biologie Moléculaire et Cellulaire (IGBMC), CNRS, INSERM, Université de
Strasbourg, UMR7104, UMR964, Illkirch, France
P-062 Harnessing e Power Of CRISPR: Generation of a diverse set of mouse
models through direct microinjection of mouse embryos (ID 126)
Michael Flores, Gary Kucera, Meilang Flowers, Cheryl Bock, Scott Soderling
Duke Cancer Institute Transgenic Core Facility, Duke University, Durham, USA
P-063 Combining CRISPR/Cas9 with Large Targeting Vectors in Mouse ES Cell
Electroporations to Produce Homozygous F0 Mice (ID 132)
Anthony Gagliardi, Gustavo Droguett, Alejo Mujica, Sean Trzaska, Charleen Hunt,
Junko Kuno, Marine Prissette, David Frendewey, Ka-Man Lai, David Valenzuela,
Wojtek Auerbach, Brian Zambrowicz
Velocigene, Regeneron Pharmaceuticals Inc., Tarrytown, NY, USA
P-064 Optimization of the Delivery of CRISPR System to Cultured Cells
and Single Cell Embryos (ID 134)
Evguenia Kouranova, Xiaoxia Cui
Horizon Discovery, St. Louis, USA
25
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
P-065 Improving the efficiency of Cas9/CRISPR genome engineering by
optimizing Cas9 delivery (ID 135)
Pawel Pelczar1, Heide Oller1, Mara Kornete2, Dietmar Schreiner3, Mario Hermann4,
Lukas Jeker2
Center for Transgenic Models, University of Basel, Switzerland; 2Department of Biomedicine, University of
Basel, Switzerland; 3Biozentrum, University of Basel, Switzerland; 4Institute of Laboratory Animal Science,
University of Zurich, Switzerland
March 20–23, 2016, Prague, Czech Republic
P-072 e Czech Centre for Phenogenomics: International research
infrastructure for mouse model production, archiving and phenotyping (ID 78)
Inken M. Beck, Trevor A. Epp, Jan Honetschlager, Jan Prochazka, Radislav Sedlacek
Czech Centre for Phenogenomics, Institute of Molecular Genetics ASCR, Prague, Czech Republic
1
P-066 Comparison of Conventional & Cloning Free Methods of Generating
CRISPR/Cas Mutant Mice (ID 136)
Paul S Devenney1, Derya D Ozdemir2, Laura A Lettice1, Nick D Hastie1, Peter
Hohenstein2
1
IGMM University of Edinburgh, UK; 2Roslin Institute University of Edinburgh, UK
P-067 Large-scale production of knockout mice using RNA-guided nucleases (ID 138)
Stephen A. Murray, Kevin A. Peterson, Leslie Goodwin, Susan Kales, Peter Kutny,
Matthew McKay, Jocelyn Sharp, Rachel Urban
e Jackson Laboratory, Bar Harbor, ME, USA
P-068 Baylor College of Medicine KOMP2 High roughput CRISPR Mouse
Production (ID 144)
Denise Lanza, John Seavitt, Isabel Lorenzo, and Jason Heaney
BaSH Consortium - Knockout Mouse Project (KOMP2) and Department of Molecular and Human Genetics,
Baylor College of Medicine, Houston TX, USA
P-069 Functional assessment of far-upstream DNA regulatory elements
of the mouse Tyr gene by CRISPR-Cas9 mutagenesis (ID 151)
Santiago Josa1,2, Davide Seruggia1,2, Almudena Fernández1,2, Rafael Jiménez1,
Marta Cantero1,2, Julia Fernández1,2, Lluís Montoliu1,2
1
National Centre for Biotechnology (CNB-CSIC) and 2CIBERER-ISCIII, Madrid, Spain
P-070 Inducible Colorectal Cancer in a Genetic Porcine Model (ID 16)
Morten M. Callesen1, Sigrid S. Árnadóttir1, Jannik E. Jakobsen2, Søren Høyer3,
Henrik Callesen4, Torben F. Ørnto1, Claus L. Andersen1 & Lars Dyrskjøt 1.
1
Department of Molecular Medicine, 2Biomedicin, 3Pathology & 4Animal Science, Aarhus University, Aarhus,
Denmark
P-071 RHEB1 expression in embryonic and postnatal mouse (ID 68)
Lev M. Fedorov1,3, Qi Tian1, James L. Smart4, Joachim H. Clement6, Yingming Wang1,
Alex Derkatch1, Harald Schubert3, Michael V. Danilchik5, Daniel L. Marks2
1
Transgenic Mouse models Shared Resource, Oregon Health & Science University, Portland, OR, USA;
Department of Pediatrics, Oregon Health & Science University, Portland, OR, USA; 3Friedrich-Schiller-University,
Jena, Germany; 4George Fox University, Newberg, OR, USA; 5Department of Integrative Biosciences, Oregon
Health & Science University, Portland, OR, USA; 6Department of Hematology & Oncology, Jena University
Hospital, Jena, Germany
2
26
P-073 Parthenogenetic activation, but not electrofusion, alters developmental
kinetics and hatching of mouse embryos (ID 18)
Marcelo Fábio Gouveia Nogueira, Pablo Diego Moço, Bruna Martins Garcia,
Elisa Mariano Pioltine, Gabriela Berni Brianezi, Letícia Rustici Chica, Lívia Vieira Saura
Department of Biological Sciences, Univ Estadual Paulista, Assis, Brazil
P-074 Comparison of the reproductive performance aer unilateral or bilateral
embryo transfer in mice (ID 22)
Esther Mahabir1, Adrian Landsberger2, Sabine Manz2, Ellen Na2, Iris Urban2, Geert Michel2
1
Comparative Medicine, Center for Molecular Medicine, University of Cologne, Germany, 2FEM, Transgenic
Technologies Charité, Berlin, Germany
P-075 Estrous cycle staging before mating increased the production of
pseudopregnant recipients without negatively affecting embryo transfer in mice
(ID 23)
Malte Heykants, Esther Mahabir
Comparative Medicine, Center for Molecular Medicine, University of Cologne, Germany
P-076 Monogamous versus polygamous mating influences the superovulation
efficiency in four strains of mice (ID 24)
Malte Heykants, Esther Mahabir
Comparative Medicine, Center for Molecular Medicine, University of Cologne, Germany
P-077 Murine norovirus is not transmitted to mice via in vitro fertilization,
intracytoplasmic sperm injection and ovary transplantation (ID 25)
Marcello Raspa1, Esther Mahabir2, Martin Fray3, Ferdinando Scavizzi1
1
National Research Council (IBCN), CNR-Campus International Development (EMMA-INFRAFRONTIER-IMPC),
Monterotondo Scalo, Italy; 2Comparative Medicine, Center for Molecular Medicine, University of Cologne,
Germany; 3Mary Lyon Centre, MRC Harwell, Harwell Science and Innovation Campus, Oxfordshire, UK
P-078 A simple and efficient vitrification procedure for cryopreserving mouse
embryos and oocytes using plastic semen straws (ID 32)
Mo Guan, Martin Fray
Medical Research Council, Mary Lyon Centre, Harwell Campus, Oxfordshire, UK
P-079 Birth of normal live mice derived mouse spermatozoa vacuum-dried
and preserved at room temperature for long term (ID 35)
Norihiro Tada1,2, Eri Nakamura1
1
Division of Genome Research, Research Institute for Diseases of Old Age; 2Division of Genome Research, Atopy
Research Center, Juntendo University Graduate School of Medicine, Tokyo, Japan
27
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
P-080 A novel non-surgical embryo transfer with TCET in mice (ID 42)
Ruonan Liu1, Yanping Miao1, Xinchong Duan1, Mo Guan2, Xiangyun Li1
1
College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China; 2Medical Research
Council, Mary Lyon Centre, Harwell Campus, Oxfordshire, UK
P-081 Using IVF to generate mice not obtainable by natural mating (ID 55)
Sheila Bryson, Seren Stedman, Laurence Cadalbert, David Stevenson,
Farah Naz Ghaffar, Douglas Strathdee
Beatson Institute for Cancer Research, CR-UK Beatson Laboratories, Garscube Estate, Switchback Road,
Bearsden, Glasgow, Scotland, UK
P-082 Generation and basic characterization of glutamate carboxypeptidase II
knock-out mice (ID 65)
Barbora Vorlova1,2, Petr Kasparek3, Pavel Sacha1,4, Radislav Sedlacek3, Jan Konvalinka1,4
1
Gilead Sciences and IOCB Research Center, Institute of Organic Chemistry and Biochemistry AS CR, v.v.i.,
Prague, Czech Republic; 2First Faculty of Medicine, Charles University, Prague, Czech Republic; 3Institute of
Molecular Genetics AS CR, v.v.i., Prague, Czech Republic; 4Department of Biochemistry, Faculty of Science,
Charles University, Prague, Czech Republic
P-083 Novel superovulation technique using inhibin antiserum and equine
chorionic gonadotropin in C57BL/6 mice (ID 84)
Toru Takeo, Ayumi Mukunoki, Naomi Nakagata
Division of Reproductive Engineering, Center for Animal Resources and Development (CARD), Kumamoto
University, Japan
P-084 e Rederivation of animals to the Francis Crick Institute (ID 107)
Katharine Mankelow, Mary Ann Haskings, Marta Miret, Ian Rosewell,
Sarah Hart-Johnson
e Francis Crick Institute, London, UK
P-085 Impaired pregnancy and birth rates in Swiss Webster mice used
for embryo transfer (ID 113)
Geraldine Schlapp, María Noel Meikle, Gabriel Fernández, Martina Crispo
P-088 An inducible Infrared Fluorescent Protein transgenic mouse line offers
alternative in vivo reporting for analysis of genetically modified models of cancer
(ID 91)
David Stevenson, Andreas K. Hock, Sheila J. Bryson, Farah Ghaffar, Seren Stedman,
Karen H. Vousden and Douglas Strathdee.
Beatson Institute for Cancer Research, Transgenic Technologies Group, Glasgow, Scotland, UK
P-089 Caspase3 defendant BRET mouse model for test of efficacy and toxicity
in new developing drugs (ID 100)
Hee-Young Yang1, Sung-Gon Kim1, Byeong-Jin Park1, Sang-Kyoon Kim1, Sang-Joon
Park2, Choong-Yong Kim1 and Gabbine Wee1
1
Laboratory Animal Center, Daegu-Gyeongbuk Medical innovation Foundation, Daegu, Korea, 2Colleage of
Veterinary medicine, Kyungpook National University, Daegu, Korea
P-090 Optogenetic tools to study cAMP signaling in cilia and flagella (ID 183)
Vera Jansen1, Jan Jikeli1, Shatanik Mukherjee2, Luis Alvarez2, Peter Hegemann3, U.
Benjamin Kaupp2, Dagmar Wachten1
1
Minerva Research Group - Molecular Physiology, Center of Advanced European Studies and Research (caesar),
Bonn, Germany; 2Department Molecular Sensory Systems, Center of Advanced European Studies and Research
(caesar), Bonn, Germany; 3Institute of Biology, Experimental Biophysics, Humboldt-Universität zu Berlin,
Germany
P-091 Imaging in animal transgenesis (ID 189)
Ryszard Slomski1,2, Magdalena Hryhorowicz1, Agnieszka Nowak1, Marlena Szalata1,
Joanna Zeyland1, Daniel Lipiński1
1
Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poland; 2Institute of Human
Genetics, Polish Academy of Sciences, Poznan, Poland
P-092 e role of Fam208a in early mouse embryogenesis (ID 13)
Shohag Bhattacharyya, Christiana Polydorou, Radislav Sedlacek, Trevor Allan Epp,
Kallayanee Chawengsaksophak
1
Transgenic and Experimental Animal Unit, Institut Pasteur de Montevideo, Uruguay
Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics, Prague, Czech Republic;
Biotechnology and Biomedicine Center of the Academy of Sciences and Charles University in Vestec, Czech
Republic
P-086 Generation of mouse lines through IVF (ID 137)
Sarah Johnson, Ying Chen, Zhenjuan Wang, Laurie Chen, and Lin Wu
P-093 A mouse model of neuronal-specific MeCP2 overexpression (ID 28)
Martha V. Koerner, Jim Selfridge and Adrian P. Bird
Genome Modification Facility, Department of Molecular and Cellular Biology, Harvard University, Cambridge,
Massachusetts, USA
Wellcome Trust Centre for Cell Biology, University of Edinburgh, UK
P-087 Generation and characterization of HLA-E transgenic pigs
for xenotransplantation (ID 190)
Magdalena Hryhorowicz1, Joanna Zeyland1, Ryszard Słomski1,2, Agnieszka Nowak1,
Marlena Szalata1, Daniel Lipiński1
1
Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Poland; 2Polish Academy of
Sciences/Institute of Human Genetics, Poznan, Poland
28
March 20–23, 2016, Prague, Czech Republic
2
P-094 Optimization of the One-Step CRISPR/Cas9 Gene Knock-Out Employing
Reporter Transgenic Zygotes (ID 37)
Ronja Apfelbaum1*, Romina Bevacqua1,3*, Wiebke Garrels1,2*, irumala R. Talluri1,
Ayan Mukherjee1, Maren Ziegler1, Birgit Burchardt1, Heiner Niemann1, Daniel Salamone3,
Ester Grueso4, Zoltan Ivics4, Wilfried A. Kues1.
1
Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany; 2Institute for Laboratory Animal
Sciences, Medical School Hannover (MHH), Germany; 3Departomento de Producción Animal, Facultad de
Agronomía, Universidad de Buenos Aires, Argentina; 4Paul-Ehrlich-Institute, Langen, Germany (*equally
contributing authors)
29
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
P-095 Influences of the gut microbiota on animal models of multiple species (ID 39)
Daniel J. Davis, Susheel B. Busi, Marcia L. Hart, Catherine H. Gillespie,
James M. Amos-Landgraf, Craig L. Franklin, Aaron C. Ericsson, Elizabeth C. Bryda
Department of Veterinary Pathobiology, University of Missouri, Columbia, USA
P-096 Knockout mouse model for human autoimmune hair loss (ID 43)
Ji-hyun Bae1, Woo-Sung Hwang1, Su-Cheong Yeom2
1
Designed Animal and Transplantation Research Institute, Greenbio Research and Technology, Seoul National
University, Daewha, Pyeongchang, kangwon, Korea; 2Graduate School of International Agricultural Technology,
Seoul National University, Daewha, Pyeongchang, Kangwon, Korea
March 20–23, 2016, Prague, Czech Republic
P-102 Generating inducible Knockout models of Cancer using CRISPR-Cas9
technology (ID 62)
Laprano N1,2, Stevenson D1, Ghaffar F1, Cadalbert L1, Bryson S1, Warrander F1,
Strathdee D1
1
e Beatson Institute for Cancer Research, Bearsden, Glasgow, Scotland, UK; 2University Of Glasgow, Institute
of Cancer Sciences, Glasgow, Scotland, UK
P-103 Function of RTEL1 in protecting cerebellar stem cells from the formation
of medulloblastoma (ID 66)
Xiaoli Wu, Sumit Sandhu, Wenjun Liu, Hao Ding
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada
P-097 Generation of various organ/tissue specific ERT2creERT2 transgenic mice (ID 44)
In-Yeong Choi1, Woo-sung Hwang1, Kyung-Jun Uh1, Dae-Kee Lee2, Su-Cheong Yeom3
1
Designed Animal and Transplantation Research Institute, Institutes of Green Bio Science and Technology,
Seoul National University, Daehwa, Pyeongchang, Gangwon, Korea, 2Ewha Womans University, Seodaemun,
Seoul, Korea, 3Graduate School of International Agricultural Technology, Seoul National University, Daehwa,
Pyeongchang, Gangwon, Korea
P-098 Function of cyclin-dependent kinase 13 during mouse development (ID 47)
Jiří Kohoutek1, Monika Nováková1, Hana Paculová1, Kallayanee Chawengsaksophak2,
Radislav Sedláček2
1
Department of Chemistry and Toxicology, Veterinary Research Institute, v.v.i., Brno, Czech Republic; 2Czech
Centre for Phenogenomics, Institute of Molecular Genetics AS CR, v. v. i., Prague, Czech Republic
P-104 Using programmable nucleases for the generation of a viable mouse
model for Netherton syndrome (ID 70)
Radka Haneckova, Petr Kasparek, Irena Jenickova, Inken M. Beck, Radislav Sedlacek
Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the ASCR, v. v. i., Prague, Czech
Republic
P-105 Strain-specific epigenetic regulation of a rat angiotensinogen transgene
in mice (ID 72)
Olga Taryma-Lesniak, Nadea Todiras, Mihail Todiras, Luiza Rabelo, Valeria Nunes,
Natalia Alenina, Michael Bader
Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
P-099 Use of CRISPR/Cas9 technology combined with ssODNs to obtain
a specific mono-allelic amino acid substitution in the TDP43 protein for
the generation of a swine model of Amyotrophic Lateral Sclerosis (ALS) (ID 48)
Corinne Quadalti1,2, Andrea Perota1, Irina Lagutina1, Giovanna Lazzari1,3,
Paola Crociara4, Maria Novella Chieppa4, Cristina Casalone4, Cristiano Corona4,
Valentina Bonetto5, Cesare Galli1,2;
Avantea-Laboratorio di Tecnologie della Riproduzione, Cremona, Italy; 2Dept.of Veterinary Medical Science,
University of Bologna, Ozzano Emilia, Italy; 3Avantea Foundation, Cremona, Italy. 4Istituto Zooprofilattico
Sperimentale PLV, Torino, Italy; 5Istituto Di Ricerche Farmacologiche Mario Negri, Milano, Italy
P-106 Nitric oxide knock out mouse is good disease model for constipation
dominant irritable bowel syndrome (ID 79)
Da-Eun Jang1, Woo-Sung Hwang2, Su-Cheong Yeom1
1
Graduate School of International Agricultural Technology, Seoul National University, Daewha, Pyeongchang,
Kangwon, Korea; 2Designed Animal and Transplantation Research Institute, Greenbio Research and Technology,
Seoul National University, Daewha, Pyeongchang, Kangwon, Korea
1
P-100 From ENU mutagenesis to knock-in reporter fusion alleles: using genetic
technologies to study the role of Fam208a (ID 50)
Veronika Grešáková1,2; Shohag Bhattacharyya1; Björn Schuster1, Inken M. Beck1,
Radislav Sedláček1, Kallayanee Chawengsaksophak1 and Trevor A. Epp1
1
Biocev, IMG AV CR v.v.i. Praha, Czech Republic; 2LF UPOL, Olomouc, Czech Republic
P-101 AHNAK deficiency promotes browning and lipolysis in mice via increased
responsiveness to β-adrenergic signaling (ID 52)
Jae Hoon Shin1, Seo Hyun Lee1, Kim Il Yong1, Kim Yo Na1, Jae-Hoon Choi2,
Hyoung-Chin Kim3, Je Kyung Seong1*
1
Korea Mouse Phenotyping Center, Laboratory of Developmental Biology and Genomics, College of Veterinary
Medicine, Seoul National University, Seoul, Korea; 2Department of Life Science, College of Natural Sciences,
Research Institute for Natural Sciences, Hanyang University, Seoul, Korea; 3Laboratory Animal Resource Center,
Korea Research Institute of Bioscience and Biotechnology, Cheongju, Korea
30
P-107 Rat Resource and Research Center (ID 85)
Elizabeth C. Bryda1, Hongsheng Men1, Yuksel Agca1, Aaron C. Ericsson1,
James M. Amos-Landgraf1, Craig L. Franklin1, Randy S. Prather2
1
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, USA;
Division of Animal Science, University of Missouri, Columbia, USA
2
P-108 Screening of transgenic and knockout mice for eye phenotypes
in the Czech Centre for Phenogenomics (ID 92)
Barbora Antosova1,2, Jitka Lachova1,2, Zbynek Kozmik1,2
1
Laboratory of Eye Biology, Institute of Molecular Genetics AS CR, v. v. i., Prague, Czech Republic; 2Czech Centre
for Phenogenomics, Institute of Molecular Genetics AS CR, v. v. i., Prague, Czech Republic
P-109 Cre-ZOO: Indispensable tool for time- and tissue- specific deletion
of genes in the mouse eye (ID 93)
Jitka Lachova, Barbora Antosova, Lucie Zilova, Anna Zitova, Radislav Sedlacek,
Zbynek Kozmik
Institute of Molecular Genetics AS CR, v. v. i., Prague, Czech Republic
31
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
P-110 Creation and characterization of the first transgenic rabbit model of long
QT5 syndrome (ID 97)
Péter Major1, István Baczkó2, László Hiripi1, Katja E. Odening3, Balázs Ördög2,
András Varró2, Zsuzsanna Bősze1
1
Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, Gödöllő, Hungary;
Department of Pharmacology & Pharmacotherapy, University of Szeged, Hungary; 3Department of Cardiology
and Angiology I, Heart Center University of Freiburg, Germany
2
March 20–23, 2016, Prague, Czech Republic
P-117 Targeting RPS19 revisited: a new mouse model for Diamond-Blackfan
Anemia (ID 118)
Björn Schuster1*, Ivan Kanchev1*, Karel Chalupsky1,2*, Jesus Ruberte3,4,5,
Jan Prochazka 1,2, Zuzana Maceckova6, Dagmar Pospisilova6, Marian Hajduch6,
Milan Reinis1,2, Anna Lastuvkova1, Henrieta Palesova1, Frantisek Spoutil1,2,
Jana Kopkanova1, Inken M. Beck1, and Radislav Sedlacek1,2†
1
Czech Centre for Phenogenomics, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic;
Dept. of transgenic models of diseases, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic;
3
Department of Anatomy and Animal Health, School of Veterinary Medicine, Universitat Autònoma de Barcelona,
Bellaterra, Spain; 4Center of Animal Biotechnology and Gene erapy, Universitat Autònoma de Barcelona,
Bellaterra, Spain; 5Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas
Asociadas (CIBERDEM), Barcelona, Spain; 6Faculty of Medicine and Dentistry, Palacky University and University
Hospital in Olomouc, Czech Republic
2
P-111 Humanising the mouse Taz Locus to allow the generation of accurate
disease models (ID 102)
Farah Ghaffar, David Stevenson, Sheila Bryson, Fiona Warrander, Douglas Strathdee
Department of Transgenic Technologies, Beatson Institute for Cancer Research, Scotland, UK
P-112 Inactivantion of Tgf-β signalling in vascular smooth muscle cells
using tissue specific inducible Cre recombinase: impact on development and
progression of aortic aneurysm in a mouse model of Marfan Syndrome (ID 108)
Paola Braghetta1, Francesco Da Ros1, Dario Bizzotto1, Daniela Carnevale2,
Giuseppe Lembo2, Giorgio Bressan1
1
Department of Molecular Medicine, University of Padua, Italy; 2Department of Angiocardioneurology and
Translational Medicine, IRCCS Neuromed, Pozzilli (IS), Italy
P-113 CARDIOGENE: Deciphering the genetic mechanisms of cardiovascular
diseases (ID 111)
Tania Sorg1, Ghina Bou-About1, Marie-France Champy1, Winfried März2, Heiko Runz3,
Mohammed Selloum1, François Spitz4, Amandine Velt1, Yann Herault1
1
Institut Clinique de la Souris-ICS-MCI, PHENOMIN, CNRS UMR7104, INSERM U964, Université de Strasbourg,
Illkirch, France; 2University of Heidelberg – Mannheim, Germany; 3University-Clinic of Heidelberg, Germany;
4
EMBL, Heidelberg, Germany
P-114 CRISPR/Cas9 Generated Mouse Models as a Tool for Rapid
Characterization of Human Disease: Confirmation of a Role for TENM1
in Congenital General Anosmia (ID 112)
Rebecca Haffner-Krausz1, Elina Berkovitz1, Golda Damari1, Sima Peretz1, Michael Tsoory1,
Tsviya Olender2, Pavlo Tatarskyy2, Valery Boyko2, Doron Lancet2, Anna Alkelai2
1
Department of Veterinary Resources, Weizmann Institute of Science, Rehovot, Israel; 2Dept. of Molecular
Genetics, Weizmann Institute of Science, Rehovot, Israel
P-115 Using an shRNA allele of Rictor mouse model to generate novel cancer
models (ID 114)
Fiona Warrander, Laurence Cadalbert, William Faller, Nicola Laprano, Sheila Bryson,
Owen Sansom, Douglas Strathdee
Beatson Institute for Cancer Research, Scotland, UK
P-116 e role of Fmr1nb in oocytes maturation and meiosis progression (ID 116)
Slavomír Kinský1, Iris Minosalva1, Kallayanee Chawengsaksophak1, Barbora Singerova1,
Andrej Susor2, Ivan Kanchev1, Trevor Epp1 and Radislav Sedláček1
P-118 ISWI ATPase Smarca5 knockout induced chromatin defects and cell cycle
progression blockade during T-cell development (ID 122)
Tomas Zikmund1, Juraj Kokavec1, Filipp Savvulidi1, Tereza Turkova1, Helena Paszekova1,
Christian Lanctot1, Arthur I. Skoultchi2, and Tomas Stopka1
1
2
Department of Pathological Physiology, 1st Faculty of Medicine, Charles University in Prague, Czech Republic;
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA
P-119 A knockin mouse with a missense mutation in the H/K-ATPase ATP4a
gene, mimics an aggressive familial form of gastric type I neuroendocrine tumors
in humans. Assessment of new therapeutic strategies (ID 127)
Oriol Calvete1,2, Pierfrancesco Vargiu3, Andrea Varro4, Marta Oteo5,
Miguel Angel Morcillo5, Alicia Barroso1, Mark Pritchard6, José Reyes7, Miriam García3,
Jaime Muñoz3, Javier Benitez1,2, * and Sagrario Ortega3, *
1
Human Genetics Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain; 2Spanish Network
of Research on Rare Diseases (CIBERER); 3Transgenic Mice Unit, National Cancer Research Centre (CNIO),
Madrid, Spain; 4Department of Physiology, University of Liverpool, UK; 5Centro de Investigaciones Energéticas,
Medioambientales y Tecnológicas (CIEMAT); 6Department of Gastroenterology, Institute of Translational
Medicine, University of Liverpool, UK; 7Department of Gastroenterology, Hospital INCA, Majorca, Spain. * JB and
SO are co-corresponding authors
P-120 Specific domain knockout of murine DNA damage-inducible protein
homolog 2 (Ddi2) (ID 128)
Monika Sivá1,2,3, Kallayanee Chawengsaksophak4, Michal Svoboda1,2, Petr Kašpárek4,
Jan Procházka4, Radislav Sedláček4, Jan Konvalinka1,2, Klára Grantz Šašková1,2
1
Institute of Organic Chemistry and Biochemistry AS CR,v.v.i., Prague, Czech Republic; 2Faculty of Science,
Charles University in Prague, Czech Republic; 3First Faculty of Medicine, Charles University in Prague, Czech
Republic; 4Institute of Molecular Genetics AS CR,v.v.i., Prague, Czech Republic
P-121 Production of new genetic tools with the help of CRISPR/Cas9
technologies to decipher THRA function in vivo (ID 141)
Denise Aubert, Romain Guyot, Suzy Markossian, Frederic Flamant
IGFL, ENS de Lyon, France
1
32
Laboratory of Transgenic Models of Diseases and Czech Centre for Phenogenomics, BIOCEV, Prague, Czech
Republic; 2Laboratory of Biochemistry and Molecular Biology of Germ Cells, Libechov, Czech Republic
33
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
P-122 EMMA: e European Mouse Mutant Archive (ID 149)
María Jesús del Hierro1, Julia Fernández1, Marta Castrillo1, Isabel Martín-Dorado1,
Lluís Montoliu1, Michael Hagn2, Fabio Mammano3, Yann Hérault4,10, Steve Brown5,
Brun Ulaake6, Jocelyne Demengeot7, Helen Parkinson8, Ramiro Ramírez-Solis9,
George Kollias11,Radislav Sedlacek12, Raija Soininen13, omas Rülicke14, Jos Jonkers15,
Fuad Iraqi16, Martin Hrabé De Angelis2
1
Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain; 2Helmholtz Zentrum München, Institute of
Experimental Genetics (HMGU-IEG), Munich, Germany; 3CNR Campus “A. Buzzati-Traverso” in Monterotondo,
Italy; 4Centre National de la Recherche Scientifique, Transgénèse et Archivage d’Animaux Modèles (CNRSTAAM), Orleans, France; 5Medical Research Council, Mammalian Genetics Unit (MRC-MGU), Harwell, UK;
6
Karolinska Institutet, Department of Cell and Molecular Biology (KI-CMB), Stockholm, Sweden; 7Fundaçaõ
Calouste Gulbenkian, Instituto Gulbenkian de Ciência, Oeiras, Portugal; 8European Molecular Biology Laboratory,
European Bioinformatics Institute (EMBL-EBI), Hinxton, UK; 9Genome Research Limited, Wellcome Trust Sanger
Institute (WTSI), Hinxton, UK; 10GIE-Centre Européen de Recherche en Biologie et en Médecine, Institut Clinique
de la Souris (GIE-CERBM-ICS), Illkirch/Strasbourg, France; 11B.S.R.C. “Alexander Fleming”, Vari/Athens, Greece;
12
Institute of Molecular Genetics (IMG), Prague, Czech Republic; 13Biocenter Oulu, Oulu, Finland; 14Biomodels
Austria (BIAT), Vienna, Austria;15Nederlands Kanker Instituut (NKI), Amsterdam, e Netherlands; 16Tel Aviv
University (TAU), Israel
March 20–23, 2016, Prague, Czech Republic
P-128 Interpreting gene regulatory information of invertebrate chordate
amphioxus: an insight from transgenic studies in zebrafish and medaka (ID 75)
Iryna Kozmikova, Zbynek Kozmik
Institute of Molecular Genetics AS CR, v. v. i., Prague, Czech Republic
P-129 Communicating Animal Research: Why and How - Lessons from Europe (ID 17)
Emma Martinez-Sanchez
e European Animal Research Association (EARA), London, UK
P-130 Mouse reproductive fitness is maintained up to an ambient temperature
of 28oC while used for producing transgenic mice (ID 46)
Jussi Helppi1, Dora Schreier1, Ronald Naumann1, Oliver Zierau2
1
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany; 2Institute of Zoology,
Technische Universität Dresden, Germany
P-131 What about the ones we don’t use? (ID 80)
Kevin Taylor
Australian BioResources, Moss Vale, Australia
P-123 A potential mouse model of sporadic AD with dysregulated neuronal
calcium homeostasis (ID 174)
Lukasz Majewski, Filip Maciag, Jacek Kuznicki
Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology in Warsaw, Poland
P-132 A low cost and high through-put mouse rederivation SOP using sperm
cryopreservation and IVF (ID 129)
Mitra Cowan, Mariette Ouellet, Andréa Barrios
Service d‘Animalerie, Centre de recherche du Centre hospitalier de l‘Université de Montréal, Canada
P-124 Using human frameshi mutations to dissect the pathobiology of ALS (ID 181)
Anny Devoy1, Julian Jaeger1, Heesoon Park1, Abraham Acevedo-Arozena2,
Elizabeth Fisher1
1
Dept of Neurodegenerative Disease, UCL Institute of Neurology, London, UK; 2MRC Mammalian Genetics Unit,
Harwell, Oxfordshire, UK
P-133 Archiving mouse lines - why archive a line? How much does it cost?
How do i submit a line for archiving? (ID 180)
Janet Kenyon and Martin Fray
Mary Lyon Centre, MRC Harwell, Harwell Campus, Oxfordshire, UK
P-125 Dopamine beta hydroxylase as a determinant of hemodynamic
and metabolic traits in the spontaneously hypertensive rats (ID 184)
Michal Pravenec1, Vladimír Landa1, Václav Zídek1, Petr Mlejnek1, Jan Šilhavý1,
Sucheta M. Vaingankar2, eodore W. Kurtz3
1
Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic; 2University of California, San
Francisco, USA; 3University of California, San Diego, USA
P-126 Generation of transgenic mice for conditional overexpression of IL-23p19
(ID 187)
Maja Kitic1, Pawel Pelczar2, omas Wunderlich3, Leonid Eshkind4, Ari Waisman1
1
Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University Mainz,
Germany; 2Center for Transgenic Models, University of Basel, Switzerland; 3Institute for Genetics, Max Planck
Institute for Metabolism Research, CECAD, Cologne, Germany; 4Transgenic Facility Mainz, Johannes Gutenberg
University, Mainz, Germany
P-127 Characterization of a novel dwarf and obese mouse line generated
by the gene-trapping method (ID 188)
Yubin Du, Wen Xie, Changyun Gui, and Chengyu Liu
34
Center for Molecular Medicine, Division of Intramural Research, National Heart, Lung, and Blood Institute,
National Institutes of Health, Bethesda, Maryland, USA
35
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
March 20–23, 2016, Prague, Czech Republic
LIST OF LATE SUBMISSIONS
GUIDELINES FOR PRESENTERS (ORAL AND POSTER)
P-134 AP-1 loss-of-function in memory formation study (ID 191)
Joanna Chilczuk, Agata Klejman, Witold Konopka
Instructions for Speakers
How to Submit Presentations at the Meeting
Please come to the Speaker’s Ready Room (STELLA) at least 1 ½ hour before
the beginning of your session. In case your speech has been scheduled for a morning
session please come to the Speakers’ Ready Room (STELLA) one day before the day of
your presentation. e Speakers’ Ready Room (STELLA) location is marked in the Final
Program.
Laboratory of Animal Models, Nencki Institute of Experimental Biology, PAS, Warsaw, Poland
P-135 PML gene overexpression and knock-out in the adult mouse brain:
application of Cre/LoxP and CRISPR/Cas9 technology (ID 192)
G. Olech1, M. Hall1, W. Konopka2, G.M. Wilczyński1
1
Laboratory of Molecular and Systemic Neuromorphology, Nencki Institute of Experimental Biology, Warsaw,
Poland; 2Laboratory of Animal Model, Nencki Institute of Experimental Biology, Warsaw, Poland
P-136 e therapeutic eff ects of sRAGE in ADPKD
Eun Ji Lee, Hyo Won Mun, Jong Hoon Park
Department of Biological Science, Sookmyung Women’s University, Seoul, Korea
P-137 Generation of genetically modified mice using the CRISPR-Cas9
technology (ID 193)
Benini Lorenzo, Benzoni Ivana, Rinaldi Rosanna, Pintonello Maria Luisa,
Ronfani Lorenza
Dibit, San Raffaele Scientific Institute, Milano, Italy
P-138 Efficient method for the isolation of functional single cell from the inner
cell mass of mouse blastocyst (ID 194)
Charles-Etienne Dumeau, Anzy Miller, Sarah Gharbi, orsten Boroviak,
William Mansfield and Brian Hendrich
e Speakers’ Ready Room is located on 3rd Floor of the Clarion Congress Hotel
Prague in the STELLA room (see floorplan on page 40).
Opening Hours for the Speakers’ Ready Room
Sunday, March 20, 2016
Monday, March 21, 2016
Tuesday, March 22, 2016
Wednesday, March 23, 2016
15:00–19:00
08:00–18:00
07:30–18:00
07:30–17:00
Instructions for Posters
Poster Board Numbers
Each poster receives a unique poster board number. e list of posters including the
poster board numbers are published in the Final Program and also available in the
poster area.
Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, UK
Poster Mounting Times
P-139 Novel Glucocorticoid-based Gene Regulation System (ID 195)
Anne Meinzinger, Peter Horvath, Leila Rachid, Tiziano Tallone, Stefan Selbert,
Doron Shmerling
PolyGene AG, Rümlang, Switzerland
P-140 One-step generation of single amino acid cohesin mutant mice
with CRISPR/Cas9 genome engineering
Anna Szydłowska, Karl Mechtler, Hans-Christian eußl, Kikuë Tachibana-Konwalski
Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria
P-141 Compairing efficiency of microinjection methods on transgenic rabbit
embryo production
Ayşe Can1, Selin Yağcioğlu1, Nilhan Coşkun1, Ramazan Arici1, Gül Bakirer Öztürk 2,
Özge Turna Yilmaz 3, Joel Marh 4, Kamber Demir1, Fatma Alev Kaymaz5, Kadir Turan6,
Mithat Evecen1, Serhat Pabuccuoğlu1, Stefan Moisyadi4, Sema Birler1
1
Istanbul University Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination,
Istanbul University Institute of Experimental Medicine, 3Istanbul University Faculty of Veterinary Medicine
Department of Obstetrics and Gynecology, 4Hawaii University John A. Burn School of Medicine, 5Istanbul University
Faculty of Veterinary Medicine Department of Internal Medicine, 6Marmara University Faculty of Pharmacy
Set-up
Dismounting
Sunday, March 20, from 15:00 until 19:00
Wednesday, March 23, until 17:00
Posters on Display
Posters will be available for viewing from 19:00 Sunday, March 20, 2016. e posters will
remain on display for the duration of the meeting and can be viewed until the specified
times:
Monday, March 21, 2016
Tuesday, March 22, 2016
Wednesday, March 23, 2016
20:00
18:00
17:00
Best Poster Prize
Posters will be evaluated during the poster session (Wednesday, March 23, 11:45–13:30)
for the best poster prize, which is generously sponsored by Charles River Laboratories
International, Inc.
2
36
37
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
March 20–23, 2016, Prague, Czech Republic
GENERAL INFORMATION
SOCIAL PROGRAM
Meeting Venue
Clarion Congress Hotel Prague
Address: Freyova 33, 190 00 Prague 9
Tel.: +420 211 131 139
www.clarioncongresshotelprague.com
Come Together Evening
Date:
Sunday, March 20, 2016
Time:
19:00–22:00
Admission:
Free for all registered participants but requires a confirmation
during the registration process.
Venue & Address:
Clarion Congress Hotel Prague, Freyova 33, Prague 9
Registration/ Information Desk
e Registration & Information Desks are located on the 3rd Floor of the Clarion
Congress Hotel Prague.
Opening hours
Sunday, March 20, 2016
Monday, March 21, 2016
Tuesday, March 22, 2016
Wednesday, March 23, 2016
15:00–20:00
08:00–18:00
08:00–18:00
08:00–18:00
Insurance
e organisers do not accept responsibility for individual medical, travel or personal
insurance. All participants are strongly advised to take out their own personal
insurance before travelling to the Meeting.
Transportation
Each registered participant receives one free public transportation pass at the
Registration Desk when registering. is ticket is valid within the dates of the Meeting.
Parking
Free parking spaces in or at the Clarion Congress Hotel Prague are not provided to
participants. Expenses for these and other parking space shall be covered by the
participants on their own.
Free WIFI
Free Wi-Fi is provided in all the meeting areas and exhibition.
SSID: TT2016 | Password: no password needed
Taxi service
In the city centre, taxis are easy to take from the street but we strongly recommend
you use hotel taxis or to call a taxi by phone through the radio taxi service.
We recommend you use following taxi companies:
AAA Taxi: +420 14 0 14
Profi Taxi: +420 14 0 15
38
We encourage you to attend the Come Together Evening and take this opportunity to
meet with colleagues and friends old and new and to visit the exhibition area.
During this evening refreshments will be served and accompanied by live music from
the accordionist Jindra Kelíšek.
Gala Dinner
Date:
Time:
Admission*:
Venue & Address:
Tuesday, March 22, 2016
19:30 – 23:30
Free for all registered participants but requires a confirmation
during the registration process.
Žofín Palace, Slovanský ostrov 226, Prague 1
*Admission with a valid ticket only. e tickets can be bought during the on-line
registration process or at the Registration Desk on-site. Please note that the capacity
of the venue is limited.
e TT2016 Gala Dinner will take place in a beautiful neorenaissance Žofín Palace, one
of the most notable architectural treasures in Prague. Žofín Palace has been a leading
centre of cultural and social life in Prague since 1837. A list of all the events would be
a very long one indeed, which testifies to the palace’s continuing importance. It is said
that anyone of real importance can be seen at Žofín frequently. It can offer Prague’s
loveliest halls, as well as top-flight services and a team that is dedicated to ensuring
satisfaction.
e “Epoque Quartet” will perform pieces from their repertoire during our gala dinner.
e Prague String Quartet comprises four excellent soloists and chamber musicians
with abundant stage experience. Winners of various international competitions, the
quartet have toured extensively throughout Europe, Japan and Israel. As compositions
of the classical quartet literature remain an integral part of the programmes of this
ensemble, their masterful approach to unconventional arrangements brings new
possibilities in using stringed instruments and at the same time enables them to
enrich their repertoire.
39
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
March 20–23, 2016, Prague, Czech Republic
EXHIBITION
LIST OF EXHIBITORS
e exhibition will be located on the 3rd Floor of the Clarion Congress Hotel Prague
and will be open at the following times:
Sunday, March 20, 2016
Monday, March 21, 2016
Tuesday, March 22, 2016
Wednesday, March 23, 2016
VENUE & FLOOR PLAN
40
16:00–20:00
08:30–18:00
08:30–17:45
08:30–18:00
Company
a-tune so ware AG
Advanced Analytical Technologies Inc.
Agnos AB
Animalab s.r.o.
Aqua-Chemie s.r.o.
BioMedical Instruments
CELL CRYOGENICS, Ltd.
Charles River
Cosmo Bio Co.Ltd.
Cryoport, Inc.
Czech Center for Phenogenomics
Envigo
Eppendorf AG
GVG Genetic Monitoring GmbH
Horizon Discovery
INFRAFRONTIER
International Product Supplies (IPS), Ltd.
Janvier Labs
Jutta Ohst german-cryo GmbH, BRD
Labotect GmbH
Omikron Systems GmbH
Planer plc
Scionics Computer Innovation GmbH
Sigma-Aldrich, a subsidiary of Merck
Sonidel
Springer Nature
Tapvei Estonia OÜ
Tecniplast S.P.A.
e International Society for Transgenic Technologies (ISTT, Inc.)
e Jackson Laboratory
Transnetyx, Inc.
Stand No.
5
16
12
8
20
26
24
2
18
28
25
17
7
29
10
25
13
4
20
6
14
19
9
15
27
21
23
1
22
3
11
41
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
SPONSORS / EXHIBITORS
March 20–23, 2016, Prague, Czech Republic
PLATINUM SPONSORS
e TT 2016 Meeting is hosted on behalf of:
e TT 2016 Meeting is hosted by:
Czech Centre for Phenogenomics
Průmyslová 595, 252 42 Vestec, Czech Republic
Charles River
Stand number: 2
Institute of Molecular Genetics AS CR, v. v. i.
Vídeňská 1083, 142 20 Prague 4, Czech Republic
Contact:
Address:
E-mail:
Website:
Mobile:
Biotechnology and Biomedicine Center of the Academy of Sciences
and Charles University in Vestec (BIOCEV)
Průmyslová 595, 252 42 Vestec, Czech Republic
42
omas Volz, Manager RM Services Europe
Charles River France, Domaine des Oncins – BP 109,
69592 L’Arbresle Cedex, FRANCE
thomas.volz@crl.com
www.criver.com/gems
+49 172 82 88 951
Charles River works with hundreds of customers globally on thousands of unique genetically engineered models to
deliver study-ready cohorts to meet research needs. We offer a wide portfolio of transgenic services including Custom
Breeding of Rats and Mice, Genetic Testing Services and Embryology Services including cryopreservation, rederivation
and rapid colony expansion. All of our breeding sites employ the Charles River Internet Colony Management (ICM™)
system. ICM™ streamlines technician workflow, simplifies project management and provides a two-way communication
portal for our customers to direct actions with their colonies, such as setting up matings, scheduling shipments and
consolidating space. At this TT2016 Meeting we are happy to announce that Charles River Europe has partnered
with Phenomin-iCS, a leader in functional genomics, to provide a complete solution for all your needs in mouse and
rat model creation. We welcome you to visit our stand and find out more about this new and exciting collaboration.
43
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
Eppendorf AG
Stand number 7
March 20–23, 2016, Prague, Czech Republic
KEY SUPPORTER
Sigma-Aldrich, a subsidiary of Merck
Stand number 15
Contact:
Address:
E-mail:
Website:
Tel:
Dr. Rudolf Walczak
Global Product Manager Cell and Liquid Handling / Portfolio Management
Barkhausenweg 1,22339 Hamburg, Germany
walczak.r@eppendorf.de
www.eppendorf.com
+49 40 53801 487
Since 1945, the Eppendorf brand has been synonymous with customer-oriented processes and innovative
products. Our portfolio comprises laboratory devices and consumables for liquid handling, cell handling and
sample handling. With more than 30 years of expertise in cell manipulation, we serve as a trusted advisor to
customers in cell biology, developmental biology and transgenic research. Our premium micromanipulation and
microinjection systems are being used in hundreds of labs worldwide and have contributed to many scientific
breakthroughs. e foundation of the company‘s expertise is its focus on its customers. e way Eppendorf
exchanges ideas with its customers results in comprehensive solutions that in turn become industry standards.
Eppendorf will continue on this path in the future, true to the standard set by the company‘s founders: that of
improving people‘s living conditions.
Contact:
Address:
E-mail:
Website:
Tel:
Peter Lindqvist, Manager – Scientific Liaison Team
Solkrasvagen 14C,135 70 Stockholm,Sweden
peter.lindqvist@sial.com
www.sigma-aldrich.com
+46 8 742 42 00
Sigma-Aldrich Corporation is a subsidiary of Merck KGaA, Darmstadt, Germany:
Taking science further faster
Merck and Sigma-Aldrich come together to empower you to focus on your work with confidence so that we can solve
the toughest problems in life science together and increase access to healthcare.
• Global network across 66 countries
• User friendly, world-class e-commerce platform
• Complete portfolio with over 300,000 products
TECNIPLAST S.P.A.
Stand number 1
Contact:
Address:
E-mail:
Website:
Tel:
Mobile:
Mr. Guglielmo Vismara
Via I Maggio, 6 – 21020 Buguggiate (Va) – Italy
guglielmo.vismara@tecniplast.it
www.tecniplast.it
+39 0332 809744
+39 366 6303049
Tecniplast, world leading company in the Lab Animal Industry since 1949, designs, manufactures and distributes
patented equipment for vivaria. We offer the most complete related product portfolio ranging from IVC (Individually
Ventilated Cages) systems, Bio containment and Bio exclusion IVCs, IVC monitoring, Analysis Systems, Aquatic
solutions, Laminar Flow, Cage and Rack washers, Bedding Handling and Disposal systems, Decontamination
and Automation, as well as a complete range of Accessories. Tecniplast has recently launched on the market
the first Digital Ventilated Cage (DVC™), enhancing the threshold in animal welfare, in users ergonomics and
in management of Animal Lab Facilities. Tecniplast supports clients in installation, training and service with a
direct presence in Italy, USA, France, UK, Germany, Australia, Japan and China, and worldwide with a network of
67 international representatives. We strive to achieve minimum environmental impact, while targeting also to
Customer Satisfaction and good working condition for collaborators.
44
45
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
EXHIBITORS
March 20–23, 2016, Prague, Czech Republic
Animalab, s.r.o.
Stand number 8
Advanced Analytical Technologies Inc.
Stand number 16
Contact:
Address:
E-mail:
Website:
Tel:
Dr. Danilo Tait
Im Neuenheimer Feld 583, 69120 Heidelberg, Germany
dtait@aati-de.com
www.aati-de.com
00491732636255
Advanced Analytical Technologies Inc. (AATI) product portfolio includes instruments for the parallel analysis
of biomolecules, DNA, RNA, genomic DNA, double-stranded DNA, gene editing (CRISPR/Cas9), pharmaceutical
compounds and proteins using capillary electrophoresis (CE) with fluorescence detection or UV absorbance.
e company’s flagship product, the Fragment Analyzer, is recognized as the best-in-class, multi-channel,
automated fluorescence-based CE detection system for the simultaneous analysis of the quantity and quality
of nucleic acids, including: dsDNA fragments, gDNA, NGS fragments and RNA (total and messenger) and
microsatellites (SSR).
Agnos AB
Stand number 12
Contact:
Address:
E-mail:
Website:
Tel:
Johan Eliasson
Agavägen 52,181 55 Lidingö,Sweden
agnthos@agnthos.se
www.agnthos.se
+46(0)87665715
“Since 1996 Agno’s has continued to expand its product range to support the biomedical research field and
life science industry. We carry a wide range of products from known suppliers to support your research from
Surgical Instruments, Stereotaxic and Behavioural Equipment to Microdialysis, Animal ID, Pumps and Surgical
Accessories.”
Contact:
Address:
E-mail:
Website:
Mobile:
Marek Baxa
Na Bateriích 505/57, 162 00 Praha 6 , Czech republic
info@animalab.cz
www.animalab.cz
+420 736 462 258
Since 2004 AnimaLab is the leading supplier in the field of biomedical research, equipment, animal facilities
and laboratories for Poland, Czech Republic and Slovakia. We supply research laboratory animal models,
animal housing equipment and wide portfolio of research equipment for behavioural, metabolic, inhalation and
physiology studies. We work with TSE, which is specializing in the development and production of systems for
behavioral phenotyping and metabolic research.
AQUA-CHEMIE spol. s r.o.
Stand number: 20
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
Petr Vávra
Freyova 82/27, 190 00 Praha 9, Česká republika
aquachemie@aquachemie.cz
www.aquachemie.cz
+420 604 272942
+420 266 721825
Exclusive representation of foreing companies PLANER plc – CHART Biomedical – Smiths Medical – Cryo Bio
System – GEA Pharma – STERIS – REVCO.
a-tune soware AG
Stand number 5
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
Alison Hopkins
Julius-Reiber-Strasse 15, 64293 Darmstadt, Germany
sales@a-tune.com
www.a-tune.com
+49 6151 951310
+49 6151 9513129
tick@lab supplied by a-tune is the soware solution for the management of Laboratory Animal Research Facilities.
For all species and transgenic breeding Colonies tick@lab manages all relevant processes for breeding, regulatory
compliance, operations and animal welfare, and provides a single point of access to data and functionality for
researchers and facility staff.
46
47
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
BioMedical Instruments
Stand number 26
Cryoport, Inc.
Stand number 28
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
Contact:
Address:
E-mail:
Website:
Tel:
Mobile:
Dr. Joachim Gündel
Zur Schönen Aussicht 26,D-07751 Zöllnitz
joachim.guendel@t-online.de
www.biomedical-instruments.com
+49-3641-395985
+49-3641-478178
Soren Knudsen
17305 Daimler St. Irvine, CA 92614
sknudsen@cryoport.com
www.cryoport.com
+1 949.232.1900
+44 (0) 7717 846 890
e BioMedical Instruments was founded in April 1997 and 2013 splittet into two companies. e BioMedical
Trading delivers equipment for scientific research, the BioMedical-Pipettes manufactures pipettes for transgenic
techniques but also custom-made pipettes for other applications in science and technology. e main aims of the
company are customer satisfaction and high quality pipettes to moderate prices.
Cryoport is the premier provider of cold chain logistics solutions to the life sciences industry through our
proprietary packaging, technology and specialized logistics expertise. We provide industry-leading shipping and
storage solutions for biologic materials, such as immunotherapies, stem cells, CAR-T cells and reproductive cells,
for biopharmaceutical, IVF and animal health organizations.
CELL CRYOGENICS LTD
Stand number 24
Envigo
Stand number 17
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
Rob orburn, Director rob.thorburn@cellcryogenics.com
St. John’s Innovation Centre, Cowley Road, Cambridge UK
rob.thorburn@cellcryogenics.com
www.cellcryogenics.com
+44(0)1223 420 252
+44(0)1223 420 844
Specialist supplier providing products for the cryopreservation of mouse embryos, embryonic stem cells, somatic
cells and sperm. Proven and highly effective mouse embryo freezing with the EF600M Controlled Rate Freezer…
LN2-free and successfully used in transgenic laboratories around the world. Optimal freezing of stem cells with
control of ‘seeding’ and also supply of special cryoprotectants.
Cosmo Bio Co., Ltd.
Stand number 18
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
48
March 20–23, 2016, Prague, Czech Republic
Hajime Aoyagi (Mr.),International Sales Dept.
2-20, Toyo 2-Chome, Koto-Ku ,Tokyo 135-0016, JAPAN
haoyagi@cosmobio.co.jp
www.cosmobio.com
+81-(0)3-5632-9617
+81-(0)3-5632-9618
Cosmo Bio provides the valuable reagents FERIUP®, CARD MEDIUM® and CARD HyperOva® to improve the recovery
of frozen mouse spermatozoa, improve in vitro fertilization efficiency and superovulate laboratory mice. We are
proud to supply a variety of products and information which allows us to serve the needs of our customers.
Dr. Peter Hepburn
PO Box 553, 5800 AN Venray, e Netherlands
Peter.hepburn@envigo.com
www.envigo.com
+31 (0) 478 578 300
+31 (0) 478 571 117
Envigo provides essential research services, models and products for biopharmaceutical, crop protection, and
chemical companies as well as universities, governments, and other research organizations. Our business is
founded on a dedication to customer service and the expertise and experience of our 3,800 people.
Horizon Discovery
Stand number 10
Address:
E-mail:
Website:
Tel:
Fax:
7100 Cambridge Research Park, Waterbeach, Cambridge, CB25 9TL,
United Kingdom
info@horizondiscovery.com
www.horizondiscovery.com
+44(0) 1223 655 580
+44(0) 1223 655 581
Horizon Discovery combines deep scientific experience in translational research with a precision gene-editing
platform incorporating rAAV, CRISPR and ZFN technologies. Horizon supplies genetically-defined cell lines, in vivo
models, custom cell line generation, molecular reference standards, and contract research services to over 1,000
academic, clinical and biopharmaceutical organizations.
49
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
March 20–23, 2016, Prague, Czech Republic
INFRAFRONTIER
Stand number 25
International Society for Transgenic Technologies (ISTT, Inc.)
Stand number 22
Address:
E-mail:
Website:
Address:
Ingolstädter Landstraße 1, 85764 Neuherberg / Munich, Germany
info@infrafrontier.eu
www.infrafrontier.eu
INFRAFRONTIER is the pan-European Research Infrastructure for generation, systemic phenotyping, archiving
and distribution of mouse disease models providing access to first-class tools and data for biomedical research.
Specialized services as generation of germ-free mice and training in state of the art cryopreservation and
phenotyping technologies complete INFRAFRONTIER service portfolio.
International Product Supplies (IPS)
Stand number 13
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
IPS Customer Services
BCM IPS LTD,London,WC1N 3XX, United Kigdom
customers@ipsltd.biz
www.ipsltd.biz
(0) 870 600 1616
(0) 870 600 1615
IPS is proud to supply Biomedical Research facilities throughout Europe with industry leading products and
services. We are exclusive European distributors of LabDiet® and TestDiet® and also produce speciality diets
such as DIO High Fat Range and AIN Series in our TestDiet®Europe facility in the UK. We are accredited to ISO
9001:2008 and ISO 14001. IPS also supplies high quality bedding and forage products and services including
irradiation, logistics and storage of items during facility refurbishments. Demand for our products and services
continue to grow so why not visit our stand (13) to see how we can assist you alternatively contact IPS on
customers@ipsltd.biz
International Society for Transgenic Technologies (ISTT, Inc.)
Roswell Park Cancer Institute,Elm and Carlton Streets,Buffalo, NY
14263, USA
E-mail:
istt@transtechsociety.org
Website:
www.transtechsociety.org
ISTT Admin.: administration@transtechsociety.org
Tel:
+1 (716) 845-5843
Fax:
+1 (716) 845-5908
e International Society for Transgenic Technologies (ISTT) seeks to: foster communication and technology
sharing, enhance scientific research, advance the field of animal transgenesis, and represent the interests of
scientists, technicians, and graduate students working in the field of transgenic technologies through electronic
exchange and by sponsoring scientific meetings, including TT2016.
e Jackson Laboratory
Stand number: 3
Contact:
Address:
E-mail:
Website:
Tel:
Wendy Reinemann
600 Main Street ,Bar Harbor Maine 04609,USA
Wendy.reinemann@jax.org
https://www.jax.org/jax-mice-and-services
207-288-6204
e Jackson Laboratory (https://www.jax.org/jax-mice-and-services) is the definitive resource for uncovering
the genetic and molecular causes of genetic diseases. Leveraging extensive partnerships with researchers and
disease-centered foundations, e Jackson Laboratory’s unparalleled mouse models and research services are
supporting the transition from discovery to application, resulting in individualized treatments and therapies
JANVIER LABS
Stand number: 4
Address:
E-mail:
Website:
Tel:
Fax:
50
CS 4105 Le Genest-Saint-Isle, F-53941 SAINT-BERTHEVIN Cedex, France
contact@janvier-labs.com
www.janvier-labs.com
+33243021191
+33243020015
Founded in 1960, JANVIER LABS is an international and independent company, specialised in rodent research
models and associated services. Our mission is to provide all International actors of Biomedical Research with the
most stable and reliable experimental materials, in order to make research easier.
51
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
Jutta Ohst german-cryo GmbH, BRD
Stand number: 20
Planer plc
Stand number: 19
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
Contact:
Address:
Email:
Website:
Tel:
Fax:
omas Henne
Silostr. 2, 41363 Jüchen, BRD, Germany
info@german-cryo.de
www.german-cryo.de
+49 2164 7003999
+49 2164 7003998
„german-cryo® has been in the cryo technology market since 2004, and has specialised in the planning, delivery
and installation of cryo systems. e basis of our advice is an exacting analysis of the requirements and technical
possibilities, with products which provide a balance between requirements and cost-effectiveness”.
Labotect GmbH
Stand number 6
Contact:
Address:
E-Mail:
Website:
Tel:
Fax:
Mrs. Anke Martin, Mr. Helge von Saltzwedel
Kampweg 12, 37124 Rosdorf, Germany.
info@labotect.com
www.labotect.com
+49 551 50501-0
+49 551 50501-11
Experience and expertise for over 40 years in the field of Assisted Reproduction! Among our products you’ll find e.g.
CO2 incubators, embryo transfer catheters, devices and disposables for aspiration and cryo-preservation. Whatever it is
you are looking for in an IVF laboratory, Labotect is the right and certified partner. Perfect quality – Made in Germany!
Omikron Systems GmbH
Stand number 14
Contact:
Address:
E-mail:
Website:
Tel:
Matthias Ottinger (DVM, PhD)
Friedrich-Seele-Str. 20,38122 Braunschweig,Germany
info@omikronsystems.de
www.omikronsystems.de
www.omikronsystems.com
+49 531 866770-0
Have a close look at LAVAN, the novel soware for your animal facility. LAVAN helps you operate your facility smoothly
and efficiently. LAVAN has best-in-class colony management tools for all species, sample tracking, visualisation of racks
and tasks, GLP-ready documentation, licence administration, order and reporting tools and more. Come visit our stand.
52
March 20–23, 2016, Prague, Czech Republic
Ravi Dattani
110 Windmill Road, Sunbury-on-ames, Middlesex, TW16 7HD, UK
enquiries@planer.com
www.planer.com
+44 1932 755000
+44 1932 755001
Planer are dedicated to using our years of expertise and experience in temperature control and the management
of cells to develop and manufacture pioneering, high quality, innovative laboratory equipment to preserve,
protect and nurture cellular biologicals. Our product range includes controlled rate freezers, incubators, alarm
& monitoring systems, sample tracking soware and storage vessels.
Scionics Computer Innovation GmbH
Stand number 9
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
Dr. Geraldine Lapeyrade
Löscherstrasse 16, 01309 Dresden, Germany
geraldine.lapeyrade@scionics.com
www.scionics.com/pyrat
+49 351 202 707 05
+49 351 202 707 04
Scionics is the creator of the soware PyRAT, which now features expanded functionality for transgenic facilities:
cryopreservation database, complete embryo cell tracking (from isolation to preservation to thawing to transfer
to transgenic offspring), and seamless integration with ordering of donors, plug checks, organ collection,
genotyping, cage card printing, etc.
Sonidel
Stand number 27
Contact:
Address:
E-mail:
Tel:
Peadar Ó’Corragáin
32 Tonlegee Drive, Raheny, Dublin 5 , Ireland
peadar@sonidel.com
+44 (0)7429266943
Supplier of advanced electroporation systems for CRISPR-Cas9, Stem Cell, KnockOut/In, TAKEN, DNA, RNA,
Zygote, GONAD, Gene erapy and Zebrafish applications - In Vivo, In Vitro, Ex Vivo and In Ovo.
53
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
Springer Nature
Stand number: 21
Contact:
Address:
E-mail:
Website:
Tel:
Fax:
Sabine Schwarz
Tiergartenstr. 17, 69121 Heidelberg, Germany.
sabine.schwarz@springer.com
www.springernature.com
+49 6221 487 8569
+49 6221 487 68569
Springer Nature is one of the world’s leading global research, educational and professional publishers, home to
an array of respected and trusted brands providing quality content through a range of innovative products and
services. Springer Nature is the world’s largest academic book publisher and numbers almost 13,000 staff in over
50 countries.
TAPVEI
Stand number 23
Contact:
Address:
E-mail:
Website:
Tel:
Julia Neglason
Paekna küla, Kiili vald,75408 Harjumaa, Estonia
Julia.neglason@tapvei.com
www.tapvei.com
+372 677 49 52
Since 1982, Tapvei has been producing the highest-quality bedding, nesting and environmental enrichment
materials for laboratory animals. Tapvei is an innovation-driven company, providing a controlled laboratory
environment for your animal research. In our waste-free production we use naturally grown Nordic aspen
(populus tremula) and renewable energy.
Transnetyx, Inc.
Stand number 11
Contact:
Address:
E-mail:
Website:
Mobile:
Fax:
54
David Porteous,European Sales Manager
8110 Cordova Road, Suite 119, Cordova, TN 38016, USA
dporteous@transnetyx.com
www.transnetyx.com
+420 724 002 070
(+1)901-507-0480
Transnetyx is the world leader in automated genotyping built on providing researchers and labs worldwide Fast,
Easy, and Accurate genotyping for animal models. Transnetyx reporting gives labs results in 72 hours guaranteed.
Using Transnetyx, researchers now have the time to focus on what matters the most – advancing their research
that leads to discoveries.
March 20–23, 2016, Prague, Czech Republic
AUTHOR INDEX
A
Aagaard Lars
21
Abdi Mehdad
19
Abe Takaya
23
Abelchian Sahar
18
Acevedo-Arozena Abraham
34
Agca Yuksel
31
Aida Tomomi
14
Alenina Natalia
31
Alkelai Anna
32
Allan Alasdair
18,21,22
Al-Saaidi RA
21
Alvarez Luis
29
Amandine Crequer
22
Amos-Landgraf James M. 30, 31
Anagnostopoulos Anna
9
Anand Mahek
19
Andersen Claus L.
26
Andre Philippe
25
Anegon Ignacio
10, 17 ,24
Angelis Fabien
21
Anjamrooz Seyed hadi
19
Antosova Barbora
31
Apfelbaum Ronja
29
Arbos Ana
24
Arici Ramazan
18
Árnadóttir Sigrid S.
26
Aubert Denise
33
Auerbach Wojtek
25
Ayabe Shinya
23
B
Baczkó István
32
Bader Michael
31
Badía C.
23
Bae Ji-Hyun
20, 30
Bai Suxia
25
Bakirer Oztürk Gül
18
Bally-Cuif Laure
19
Barbero Jose Luis
20
Barde Isabelle
21
Barrios Andréa
35
Barroso Alicia
33
Batton Jeff
13
Beck Inken
24, 27, 30, 31, 33
Becker Celine
22
Beckett Caroline
10
Bedu Sébastien
19
Beechey Colin
16
Behringer Richard
13
Benitez Javier
33
Berkovitz Elina
32
Bevacqua Romina
29
Bhattacharyya Shohag
29, 30
Biechele Steffen
17
Biggs Daniel
21
Bird Adrian P.
29
Birler Sema
18
Birling Marie-Christine
14, 16,
21,25
Bizzotto Dario
32
Bock Cheryl
25
Boehm omas
14
Bolund Lars
21, 22
Bonderup Kasper
18
Bonetto Valentina
30
Boroviak Katharina
13, 24
Bosch Fàtima
24
Bősze Zsuzsanna
32
Bottomley Joanna
20
Bou-About Ghina
32
Bout Henriette
12
Bovolenta Matteo
22
Boyko Valery
32
Braghetta Paola
32
Brakebusch Cord
10, 18
Bressan Giorgio
32
Brianezi Gabriela Berni
27
Brickman Joshua
18
Bringart Matthieu
21
Brody Leigh
19
Brøgger Peter
21
Brønstad Aurora
12
Bross Peter
21
Brown Ellen
20, 24
Brown Steve
34
Brusselle Lucas
17, 24
Bryda Elizabeth
30, 31
Bryson Sheila
28, 29, 31, 32
Burchardt Birgit
29
Busi Susheel B.
30
C
Cadalbert Laurence
Cai Bei
Cailleau Catherine
Callesen Henrik
Callesen Morten
Calvete Oriol
Can Ayşe
Cantero Marta
Capoano Adrián
Carnevale Daniela
Casalone Cristina
Caspary Tamara
Castrillo Marta
28, 31, 32
17
22
26
26
33
18
26
25
32
30
16
18, 34
Caulder Adam
16, 21, 22
Cebrian-Serrano Alberto
21
Chalupsky Karel
33
Champy Marie-France
32
Chang Isabelle
18
Chang Sheng-Kai Chang
19
Charton Karine
22
Chauveau-Le Friec Gaëlle
25
Chawengsaksophak Kallayanee 29,
30, 32, 33
Chen Laurie
28
Chen Wei-Nien
19
Chen Ying
28
Chen Yinhuai
18
Chen Yulin
17
Chenouard Vanessa
17
Chen-Tsai Ruby Yanru
13
Chica Letícia Rustici
27
Chilczuk Joanna
36
Choi In-Yeong
20, 30
Choi Jae-Hoon
30
Chou Tzu-Yu
19
Clement Joachim H.
26
Codner Gemma 16, 18, 21, 22, 23
Colleoni Silvia
24
Concordet Jean-Paul
20, 21, 24
Corona Cristiano
30
Cowan Mitra
35
Cozzi Jean
10
Crispo Martina
25, 28
Crociara Paola
30
Crotti Gabriella
24
Cui Xiaoxia
25
D
Dahlem Timothy
Damari Golda
Danilchik Michael V.
Da Ros Francesco
Davies Benjamin
Davis Daniel
De Cian Anne
Del Hierro Maria Jesus
Delerue Fabien
Demengeot Jocelyne
Demir Kamber
Denda Atsumi
Derkatch Alex
de Villartay Jean-Pierre
Devenney Paul
Devoy Anny
Dickinson Mary
Dillard Victor
22
32
26
32
21
30
24
18, 34
20
34
18
23
26
22
26
34
10
19
55
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
Ding Hao
31
Dixon John
18
Dobrowolski Peter
16
Doe Brendan
20, 23, 24
Doyle Riley
19
Dray Nicolas
19
Dreyer Dominique
25
Droguett Gustavo
25
Du Yubin
34, 36
Duan Xinchong
28
Duboule Denis
15
Duchi Roberto
24
Duddy Graham
20, 23, 24
Duelund Pedersen Henrik
21
Dwinell Melinda
24
Dyrskjøt Lars
26
E
Ebrahimi Ayyub
19
Eckert Helene
20
Eghbalsaied Shahin
17
Eisenmann Benjamin
16, 25
Epp Trevor A.
27, 29, 30, 32
Erbs Valérie
25
Ericsson Aaron C.
30, 31
Erlebacher Adrian
22
Eschstruth Alexis
20
Eshkind Leonid
34, 36
Evans Edward
16
F
Falcone Alexandra
18
Faller William
32
Fathi Fardin
19
Fedorov Lev
26
Felipe Natalia
20
Fell Rachel
22, 22
Fernandez Almudena
26
Fernandez Gabriel
28
Fernandez Julia
18, 26, 34
Filipiak Wanda
22
Fiore Frederic
21
Fischer Melina
16
Fisher Elizabeth
34
Flamant Frédéric
20, 33
Flores Michael
25
Flowers Meilang
25
Foeckler Jamie
24
Foucher Isabelle
19
Franklin Craig L.
30, 31
Fray Martin
21, 22, 27, 35
Frendewey David
25
Fuertes-Alvarez Sandra
19
Furuta Yas
23
56
March 20–23, 2016, Prague, Czech Republic
G
Gagliardi Anthony
25
Gale Nicholas
10
Galli Cesare
24, 30
Gantz Valentino
13
Garcia Bruna Martins
27
Garcia Miquel
24
Garcia Miriam
33
Garcia-Tunon Ignacio
20
Gardiner Wendy
21, 22
Garrels Wiebke
12, 29
Gaspero Angelina
23
Gatti Sara Ortica
19
Gavrilina Galina
22
Geisinger Adriana
25
Gelsen Olaf
16
Gersbach Charles
9
Gertsenstein Marina
10, 17, 22
Geurts Aron M.
24
Ghaffar Farah
29, 31, 32
Giacalone Joseph
17
Gillespie Catherine H.
30
Giovannangeli Carine
24
Gleeson Diane
20, 24
Gocza Elen
19
Gök Mehmet Koray
18
Golovko Andrei
9
Gómez M.
23
Gomez-H Laura
20
Goodwin Leslie
23, 26
Gouveia Nogueira Marcelo Fábio 27
Grantz Šašková Klára
33
Grau Evelyn
24
Grešáková Veronika
30
Grove Erin
17
Grueso Ester
29
Grzybowski Michael
24
Guan Mo
27, 28
Gui Changyun
34, 36
Guo Caiying
25
Gurumurthy CB
16, 23
Guyot Romain
33
H
Haeussler Maximilian
Haffner-Krausz Rebecca
Hagn Michael
Hajduch Marian
Hajihoseini Zohreh
Hamra F. Kent
Hanečková Radmila
Harscoet Erwana
Hart Marcia L.
Hart-Johnson Sarah
Haskings Mary Ann
20
32
34
33
17
14
31
21
30
28
28
Hastie Nick D.
Heaney Jason
Hegemann Peter
Hejnar Jiri
Helppi Jussi
Henriques Sara F.
Herault Yann
26
23, 26
29
24
35
22
13, 16, 21, 25,
32, 34
Hermann Mario
26
Heykants Malte
27
Hines Marcus
22
Hiripi László
32
Hock Andreas K.
29
Hohenstein Peter
26
Honetschlager Jan
27
Hope Sandra
13
Høyer Søren
26
Hrabe De Angelis Martin
34
Hryhorowicz Magdalena 28, 29, 36
Hu Yueh-Chiang
18
Huang Xingxu
17
Huang Yao
16
Huang Zu-Shan
19
Hübner Christian
23
Hughes Elizabeth
22
Humphryes Neil
19
Hunt Charleen
25
Hussmann Dianna
21
Hwang Woo-Sung
20, 30, 31
I
Ileninova Zuzana
Ingenito Joanne
Iraqi Fuad
Isaka Mayumi
Ittner Lars
Ivics Zoltan
J
Jagla Térésa
Jacob Howard J.
Jaeger Julian
Jakobsen Jannik E.
Jang Da-Eun
Jansen Vera
Jarrold James
Jeker Lukas
Jenickova Irena
Jeong Yeong-Hee
Jikeli Jan
Jiménez Rafael
Johnson Sarah
Johnson omas
Joly Jean-Stephane
Jonkers Jos
17
17
34
17
20
29
21
24
34
26
20, 31
29
21, 22
26
24, 31
20
29
26
28
17
20, 21
34
Josa Santiago
Jurisicova Andrea
26
17
K
Kales Susan
23, 26
Kalloway Shawn
24
Kanchev Ivan
17, 32, 33
Kaneko Mari
23
Kanzler Benoit
17
Kasparek Petr 14, 17, 24, 28, 31,
33
Katidou Markella
18
Kaucher Amy
12
Kaupp Benjamin
29
Kent Jim
20
Kenyon Janet
35
Khan Mona
18
Kim Choong-Yong
29
Kim Hyoung-Chin
30
Kim Sang-Kyoon
29
Kim Sang Yong
22
Kim Sung-Gon
29
King Ruairidh
21, 22
Kinský Slavomír
32
Kitic Maja
34, 36
Kiyonari Hiroshi
23
Klein Tino
21
Klejman Agata
36
Klotz Jason
24
Kocabay Ahmet
19
Koentgen Frank
18
Koerner Martha
29
Kohoutek Jiří
30
Kokavec Juraj
33
Kollias George
34
Kong Lingjie
18
Konopka Witold
36
Konvalinka Jan
28, 33
Kopkanová Jana
33
Koralov Sergei
22
Kornete Mara
26
Kouranova Evguenia
25
Kozmik Zbynek
31, 35
Kozmikova Iryna
35
Kreiner Grzegorz
9
Kucera Gary
25
Kucerova Dana
24
Kues Wilfried
29
Kuhn Ralf
9
Kuno Junko
25
Kuo Chieh-Ti
24
Kurth Ingo
23
Kurtz eodore W.
34
Kutny Peter
26
Kuznicki Jacek
34
L
LaForest Anna
Lafosse Ilta
Lagutina Irina
Lachova Jitka
Lai Ka-Man
Lancet Doron
Lanctot Christian
Landa Vladimir
Landsberger Adrian
Langa Francina
Lanza Denise
Laprano Nicola
Larson Melissa
Lastuvkova Anna
Lazar Bence
Lazar Jozef
Lazcares Lynn
Lazzari Giovanna
Lee Dae-Kee
Lee Eun Ji
Lee Seo Hyun
Lembo Giuseppe
Lemke Angela
Lettice Laura A.
Leu Adrian
Levy David
Lindner Loic
Lipinski Daniel
Lipson Ruffert Karelia
Li Jinling
Li Xiangyun
Lin Jiangwei
Lin Lin
Lin Shu-Wha
Lin Wei-Jou
Ling Huang-Yi
Lipiński Daniel
Liu Chengyu
Liu Ruonan
Liu Wenjun
Liu Yong
Llano Elena
Lloyd Kent
Loeffler Jorik
Lorentz Romain
Lorenzo Isabel
Lounkova Anna
Lovell-Badge Robin
Luo Yonglun
Luo Jinping
M
Ma Baohua
Maceckova Zuzana
22
25
24, 30
31
25
32
33
34
27
21, 25
23, 26
31, 32
9
33
19
24
24
24, 30
30
37
30
32
24
26
17
22
16
28, 29, 36
21, 22
18
28
18
21, 22
19
19
19
28, 36
34, 36
28
31
21, 22
20
10, 23
18
25
26
24
14
12, 21, 22
22
17
33
Maciag Filip
34
Maehr Rene
15
Maeso-Alonso Laura
19
Mahabir Esther
27
Majewski Lukasz
34
Major Peter
32
Mammano Fabio
34
Mankelow Katharine
28
Manz Sabine
27
Manzanares M.
23
Maraghechi Pouhneh
19
Marin Maria
19
Maritati Marina
18, 21, 22
Mark Willie
17
Marks Daniel L.
26
Markossian Suzy
20, 21, 33
Marques Margarita
19
Martin Susan
18
Martín-Dorado Isabel
18, 34
Martín-González Javier
14, 18
Martin-Lopez Marta
19
Martinez-Sanchez Emma
35
März Winfried
32
Matthews Peter
23
McKay Matthew
26
McLaughlin K. John
17
Meehan Terrence
23
Meikle Maria Noel
25, 28
Men Hongsheng
31
Ménoret Séverine
9, 17, 24
Mercier Pascale
21
Mertz Annelyse
16
Mianne Joffrey 16, 18, 21, 22, 23
Miao Yanping
28
Michel Geert
27
Miller Jocelyn
24
Miret Marta
28
Miura Hiromi
23
Mlejnek Petr
34
Moço Pablo Diego
27
Moghaddasi Saeedeh
17
Moigneu Carine
25
Mojica Francis
11
Mombaerts Peter
18
Montoliu Lluis
9, 18, 26, 34
Montoya Guillermo
20
Morcillo Miguel Angel
33
Morgani Sophie
18
Mosimann Christian
9
Moussu Jean-Paul
22
Mukherjee Ayan
29
Mujica Alejo
25
Mukherjee Shatanik
29
Mukumoto Yoshiko
23
Mukunoki Ayumi
28
57
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
Mulet Ana Paula
Mun Hyo Won
Muñoz Jaime
Murray Stephen
25
37
33
23, 26
N
Na Kim Yo
30
Nagy Andras
8
Nakagata Naomi
18, 28
Nakamura Eri
27
Naumann Ronald
16, 35
Naz Ghaffar Farah
28
Nemeth Kinga
19
Nguyen Tuan Huan
24
Nielsen Anders Lade
21
Niemann Heiner
29
Niu Yiyuan
17
Nováková Monika
30
Novella Chieppa Maria
30
Nowak Agnieszka
28, 29, 36
Nunes Valeria
31
Nutter Lauryl M. J. 10, 17, 22, 23
O
Obata Yuichi
Odening Katja E.
Offner Sandra
Ohtsuka Masato
Olech G.
Olender Tsviya
Oller Heide
Onder Tevfik Tamer
Ördög Balázs
Ørnto Torben F.
Ortega Sagrario
Oteo Marta
Ouellet Mariette
Ouisse Laure-Hélène
Ozdemir Derya D
58
23
32
21
14, 16, 23
36
32
26
19
32
26
33
33
35
17
26
P
Pabuccuoğlu Saadet K.
18
Pabuccuoğlu Serhat
18
Paculová Hana
30
Palesova Henrieta
17, 33
Park Byeong-Jin
29
Park Jong Hoon
37
Park Sang-Joon
29
Park Heesoon
34
Parkinson Helen
34
Paszekova Helena
33
Pavlovic Guillaume 10, 16, 21, 25
Pelczar Pawel
26, 34, 36
Pendas Alberto M.
20
Peretz Sima
32
Perota Andrea
24, 30
Peterson Kevin A.
26
Peterson Kevin A.
13, 23
Pioltine Elisa Mariano
27
Piotrowska-Nitsche Karolina 16
Plachy Jiri
24
Polydorou Christiana
29
Pospisilova Dagmar
33
Pravenec Michal
34
Preece Chris
21
Premsrirut Prem
14
Prissette Marine
25
Pritchard Mark
33
Prochazka Jan
27, 33
Proudfoot Chris
12
Pu Feng-Lin
19
Pujol Anna
24
Q
Qian Zhou Guang
21
Qu Lei
17
Quackenbush Teresa
16
Quadalti Corinne
24, 30
Quadros Rolen
23
Quillaud-Chenouard Vanessa 24
R
Rabelo Luiza
31
Radage Adam
16
Rahaee Ahmadreza
17
Ramirez-Solis Ramiro 20, 23, 24,
34
Raspa Marcello
27
Rayón T.
23
Reinis Milan
33
Reinisova Marketa
24
Remy Séverine
17, 24
Reyes José
33
Rezaei Mohammad Jafar
19
Richard Isabelle
22
Risson Valérie
20, 21
Rollán I.
23
Romanienko Peter J.
17
Rønn Sif Groth
21
Rosen Barry
20
Rosewell Ian
28
Ross Pablo
15
Rossant Janet
17, 22
Rostamzadeh Jalal
19
Ruberte Jesus
33
Rülicke omas
34
Runz Heiko
32
Ryder Ed
20, 23, 24
March 20–23, 2016, Prague, Czech Republic
S
Sabol Maja
24
Sacha Pavel
28
Salamone Daniel
29
Sandhu Sumit
31
Sansom Owen
32
Sato Masahiro
16, 23
Saunders omas
22
Saura Lívia Vieira
27
Savvulidi Filipp
33
Scavizzi Ferdinando
27
Schilling Rebecca
24
Schimenti John
14, 20
Schlapp Geraldine
25, 28
Schmitt Philippe
21
Schneider-Maunoury Sylvie
20
Schönig Kai
20
Schorr Katrin
23
Schreier Dora
35
Schreiner Dietmar
26
Schubert Harald
26
Schuster Bjoern
24, 30
Scott Melissa A.
18
Seavitt John
26
Seavitt John
23
Sedlacek Radislav 17, 24, 27, 28,
29, 30, 31, 32, 33, 34
Selfridge Jim
29
Selloum Mohammed
32
Seong Je Kyung
30
Seruggia Davide
26
Sethi Debarati
20
Severinov Konstantin
11
Sharp Jocelyn
26
Shin Jae Hoon
30
Shkumatava Alena
20
Singerova Barbora
32
Sivá Monika
33
Skoultchi Arthur I.
33
Skov Petersen Trine
21, 22
Slomsk Ryszard
29
Słomski Ryszard
28, 36
Smart James L.
26
Soderling Scott
25
Soininen Raija
34
Sørensen Charlotte Brandt
21
Sorg Tania
32
Spitz François
32
Spoutil Frantisek
33
Spruston Nelson
25
Stainier Didier
13
Stedman Seren
28, 29
Stevens Sean
12
Stevenson David
28, 29, 31, 32
Stewart Michelle
12
Stopka Tomas
33
Strathdee Douglas 28, 29, 31, 32
Su Xin
25
Suel Laurence
22
Sun Amy
22
Susor Andrej
32
Svoboda Michal
33
Sylvie Jacquot
25
Szalata Marlena
28, 29, 36
Šilhavý Jan
34
T
Tada Norihiro
27
Tai Jimmy
18
Taillepierre Miguel
22
Takeo Toru
13, 18, 28
Takizawa Akiko
24
Talluri irumala R.
29
Tamowski Susan
22
Tan Shuang
21
Taryma-Lesniak Olga
31
Taskin Ali Cihan
19
Tatarskyy Pavlo
32
Taylor Kevin
35
Teboul Lydia
16, 18, 21, 22, 23
Teixeira Marie
20
Temple Anne
24
Tesson Laurent
17, 21, 24
omas Mark
20
Tian Qi
26
Todiras Nadea
31
Todiras Mihail
31
Tomni Cecilia
23
Trzaska Sean
25
Tsai Pi-Fang
19
Tsai Ching-Yen
19
Tsoory Michael
32
Tu Yi-Fang
19
Turini Paola
24
Turkova Tereza
33
Turón Sandra
24
U
Uh Kyung-Jun
Ulaake Brun
Urban Rachel
Urban Iris
Usal Claire
V
Vaingankar Sucheta M.
Valenzuela David
Vargiu Pierfrancesco
Varro Andrea
Varró András
20, 30
34
26
27
17, 24
34
25
33
33
32
Velt Amandine
Von Schiller Dominique
Vorlova Barbora
Vousden Karen H.
32
20
28
29
W
Wachten Dagmar
29
Waisman Ari
34, 36
Wang Haoyi
14
Wang Xiaolong
17
Wang Yijie
17
Wang Sheng-Wen
19
Wang Cheng-Ju
19
Wang Yingming
26
Wang Zhenjuan
28
Wardle-Jones Hannah
24
Warrander Fiona
31, 32
Watanabe Satoshi
16
Watanabe Yoshi
20
Watase Megumi
23
Wattenhofer-Donze Marie 16, 25
Watts Jacqui
18
Wee Gabbine
29
Weiss Ron
15
Wells Sara
21, 22, 23
Wilczyński G.M.
36
Willis Brandon
23
Wolf Eckhard
12
Woods Michael
20
Woods Mike
24
Wu Po-Yueh
19
Wu Chien-Hui
19
Wu Lin
28
Wu Xiaoli
31
Wunderlich omas
34, 36
X
Xie Huirong
Xie Wen
18
34, 36
Y
Yamashita Yui
Yan Lin
Yang Hee-Young
Yao Xiaohao
Yeom Su-Cheong
Yong Kim Il
Yong Kim Sang
Yoshiki Atsushi
You You
Yu I-Shing
Yum SooYoung
23
18
29
25
20, 30
31
23
23
17
19
12
Z
Zambrowicz Brian
25
Zappa Allison
Zare Sonia
Zeidler Michael
Zetsche Bernd
Zeyland Joanna
Zhang Shuqin
Zhao Xianling
Zhao Shumei
Zheng Qi
Zhou Yan
Zhou Ping
Zídek Václav
Ziebell Corey
Ziegler Maren
Zierau Oliver
Zikmund Tomas
Zilova Lucie
Zitova Anna
24
19
22
11
28, 29, 36
25
25
25
18
21, 22
22
34
22
29
35
33
31
31
59
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
TT2016 PARTICIPANTS LIST
60
First Name
Antony
Lisbeth
Younes
Tomomi
Aytekin
Christophe
Natalia
Samy
Rahmen
Alasdair
Emma
Elisa
Anna
Mahek
Konstantinos
Sara
Philippe
Ignacio
Barbora
Ronja
Ferran
Anna
Tanja
Patricia
Vida
Sonja
Denise
Wojtek
Abdelkader
Michael
Gül
Petr
Jeff
Aline
Lenegereshe
Inken
Katja
Nadine
Caroline
Richard
Ambra
Lorenzo
Elina
Dierk
Pietro
Hartmut
Shohag
Chunming
Kiril
Daniel
Marie-Christine
Lorraine
Michelle
omas
Cheryl
Annemette
omas
Kasper
Surname
Adamson
Ahm Hansen
Achouri
Aida
Akyol
Alberti
Alenina
AlGhadban
Ali
Allan
Allan
Allievi
Anagnostopoulos
Anand
Anastassiadis
Andersson
Andre
Anegon
Antosova
Apfelbaum
Aragon Manresa
Arbos
Artz
Arubaleze
Asghari
Assenmacher
Aubert
Auerbach
Ayadi
Bader
Bakırer Öztürk
Bartunek
Batton
Baur
Baweke
Beck
Becker
Becker
Beckett
Behringer
Belpietro
Benini
Bercovitz
Berger
Bernardini
Berns
Bhattacharyya
Bi
Bidzhekov
Biggs
Birling
Blackford
Blom
Blom
Bock
Boe Marnow
Boehm
Bonderup
Organisation, City, Country
University of Manchester, Manchester, U.K.
Aarhus University, Aarhus C, Denmark
Université catholique de Louvain, Brussels, Belgium
Tokyo Medical and Dental University, Tokyo, Japan
Hacettepe University, Ankara, Turkey
Institut Curie Centre de Recherche, Orsay Cedex, France
MDC, Berlin, Germany
University of Leicester, Leicester, U.K.
e Netherlands Cancer Institute, Amsterdam, e Netherlands
C/O UK Shared Business Services Ltd, Swindon, U.K.
University Of Edinburgh, Edinburgh, U.K.
Cogentech S.c.a.r.l., Milan, Italy
e Jackson Laboratory, Bar Harbor, Maine, USA
Szent Istvan University, Godollo, Hungary
Technische Universitaet Dresden, Dresden, Germany
Lund University, Lund, Sweden
CERBM-GIE, Illkirch, France
INSERM UMR 1064/Platform TRIP, Nantes, France
Institute of Molecular Genetics of the ASCR, v. v., Praha 4, Czech Republic
Friedrich-Loeffler-Institute, Neustadt, Germany
Institute for Research in Biomedicine, Barcelona, Spain
Universitat Autònoma de Barcelona, Bellaterra, Spain
University Bonn, Bonn, Germany
International Society for Transgenic Technologies, Buff ao, USA
Genentech, Inc., South San Francisco, USA
Universität zu Köln, Cologne, Germany
IGFL CNRS UMR5242-ENS-UCBL-INRA1288, Lyon cedex 07, France
Regeneron Pharmaceuticals, Tarrytown, USA
GIE-CERBM , Institu Clinique de la Souris, Illkirch, France
MDC, Berlin, Germany
istanbul University, istanbul, Turkey
Institute of Molecular Genetics, Prague 4, Czech Republic
GeneSearch, Inc., Bozeman, USA
Novartis Pharma AG, Basel, Switzerland
NIMH/NIH, Bethesda, USA
Institute of Molecular Genetics ASCR, Prague, Czech Republic
Max-Delbrück-Centre, Berlin, Germany
Universität des Saarlandes, Homburg, Germany
Sigma-Aldrich International GmbH, Gallen, Switzerland
University of Texas MD, Houston, USA
Cogentech S.c.a.r.l., Milan, Italy
Ospedale San Raff aele srl, Milano, Italy
weizmann institute of science, Rehovot, Israel
Taconic Biosciences, Cologne, Germany
TECNIPLAST SPA, Buguggiate (Va), Italy
St. Jude Children‘s Research Hospital, Memphis, USA
Institute of Molecular Genetics, Praque, Czech Republic
University of Pittsburgh, Pittsburgh, USA
LMU München, München, Germany
University of Oxford, Oxford, U.K.
CERMB-GIE, Illkirch, France
Roslin Institute, Midlothian, U.K.
EPFL SV CPG-GE, Lausanne, Switzerland
Lund University, Lund, Sweden
Duke Medical Center, Durham, USA
Aarhus University, Aarhus C, Denmark
Max Planck Institute of Immunobiology & Epigenetic, Freiburg, Germany
Univeristy of Copenhagen , Panum, Copenhagen, Denmark
March 20–23, 2016, Prague, Czech Republic
Katharina
Markus
Franck
Henriette
Paola
Sebastian
Cord
Jan Ove
Karen
Gunnel
Aurora
Conny
Ellen
Lucas
Elizabeth
Sheila
Sandra
orsten
Alexa
Morten M.
Marta
Mariano
Roxanne
Marta
Alberto
Catarina
Caroline
Nicole
Kallayanee
Ruby Yanru
Joanna
Tzu-Yu
Krzysztof
Jill
Gemma
Talia
Luisa
Nuno
Mitra
J. Colin
Jean
Martina
Anna
Carme
Michael
Golda Damari
Philip
Fabien
Amarnath
Celine
Ben
Daniel
Ana
Francesca
Mariann
Jessica
Maria Jesus
Fabien
Ke-Yu
Paul
Anny
Silvia
Boroviak
Bosmann
Bourgade
Bout
Braghetta
Brachs
Brakebusch
Bratteng
Brennan
Brolin
Brønstad
Brouwers
Brown
Brusselle
Bryda
Bryson
Buhl
Buch
Burger
Callesen
Cantero
Cao
Carreer
Castrillo
Cebrian-Serrano
Certal
Chabert-Le Borgne
Chambers
Chawengsaksophak
Chen-Tsai
Chilczuk
Chou
Chylinski
Claassens
Codner
Cohen
Corsi
Costa-Borges
Cowan
Cox
Cozzi
Crispo
Crowley
Cucarella
Curley
Damarigo
Damiani
Danjan
Dasari
Daviaud
Davies
Davis
de Castro
De Giorgio
De Jaeghere
Del Bravo
del Hierro
Delerue
Deng
Devenney
Devoy
Di Meglio
e Wellcome Trust, Sanger Institute, Cambridge, U.K.
Unimedizin Mainz, Mainz, Germany
Institut Pasteur, Paris, France
ConScience, Amsterdam, e Netherlands
University of Padova, Padova, Italy
Charité - Universitätsmedizin Berlin, Berlin, Germany
University of Copenhagen, Copenhagen, Denmark
Karolinska Institutet, Stockholm, Sweden
e Victor Chang Cardiac Research Institute, Darlinghurst, Australia
Karolinska Institutet, Huddinge, Sweden
University of Bergen, Bergen, Norway
Leiden University Medical Center, Leiden, e Netherlands
Wellcome Trust Sanger Institute, Hinxton, U.K.
INSERM UMR 1064/Platform TRIP, Nantes, France
University of Missouri, Columbia, USA
Beatson Institute for Cancer Research, Brearsden, Glasgow, U.K.
Taconic Biosciences GmbH, Cologne, Germany
University of Zurich, Schlieren, Switzerland
University of Zurich, Zürich, Switzerland
Aarhus University Clinical Medicine, Aarhus N, Denmark
Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain
Kheiron SA, Caba, Argentina
Partnering4Biotech, Wezembeek-Oppem, Belgium
Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain
Oxford University, Oxford, U.K.
Champalimaud Foundation, Lisboa, Portugal
Pharming group N.V. branch, Evry Cedex, France
Institute of Molecular Genetics of the ASCR, v. v., Praha, Czech Republic
Institute of Molecular Genetics, Prague 4, Czech Republic
Applied StemCell, Inc., USA
Nencki Institute of Experimental Biology PAS, Warsaw, Poland
Academia Sinica, Taipei, Taiwan
VBCF, Wien, Austria
Leiden University Medical Center, Leiden, e Netherlands
Medical Research Council C/O UKSBS, Swindon, U.K.
Techinion, Haifa, Israel
Envigo RMS Srl, Bresso, Italy
Embryotools, Barcelona, Spain
CRCHUM, Montreal, Canada
Genentech, South San Francisco, USA
Charles River, Wilmington, Boston, USA
Institut Pasteur de Montevideo, Montevideo, Uruguay
UCL, London, U.K.
Instituto de Biomedicina de Valencia, Valencia, Spain
University of Edinburgh, Edinburgh, U.K.
Weizmann Institute of Science, Rehovot, Israel
Consultant, Staten Island, USA
Ciphe, Marseille Cedex 09, France
Taconic Biosciences, Rensselaer, USA
Institut Curie Centre de Recherche, Paris Cedex 05, France
University of Oxford, Oxford, U.K.
University of Missouri, Columbia, USA
INFRAFRONTIER GmbH, Neuherberg Muenchen, Germany
UCL Institute of Neurology, London, U.K.
Max Planck Institute for biology of aging, Cologne, Germany
Netherlands Cancer Institute, Amsterdam, e Netherlands
Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain
University of New South Wales, Kensington, Australia
Nanchang University, Nanchang, China
MRC IGMM at the University of Edinburgh, edinburgh, U.K.
UCL Institute of Neurology, London, U.K.
Sigma-Aldrich International GmbH, Gallen, Switzerland
61
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
62
Mary
Hao
Andras
John
Sascha
Peter
Brendan
Lynn
Tomáš
Joanne
Deborah
Bernadette
Yubin
Denis
Charles-Etienne
Michael
Marianne
Shahin
Marc
Fatima
Ferenc
Leonid
Kristin
Katerina
Fardin
Lev
Anastasia
Rachel
Julia
Steve
Judith
omas
Peter
Dieter
Frederic
Elizabeth
Michael
Jamie
Sandra
Jiri
Stephen
Eirini
Martin
Robert
Marc
Ariana
Ernst-Martin
Ursula
Yas
Anthony
Juan
Nicholas
Kevin
Lin
Valentino
Mickael
Wiebke
Olaf
Charles
Marina
Andrei
Guochun
Dickinson
Ding
Dinnyes
Dixon
Dlugosz
Dobrowolski
Doe
Doglio
Doležal
Doran
Drage
Drayton
Du
Duboule
Dumeau
Durnin
Eckert
Eghbalsaied
Ekker
El Marjou
Erdélyi
Eshkind
Evans
Faltusova
Fathi
Fedorov
Felker
Fell
Fernandez
Festin
Fiedler
Fielder
Filipčík
Fink
Fiore
Fisher
Flores
Foeckler
Fontaniere
Forejt
Forrow
Fragkiadaki
Fray
Fredriksson
Freichel
Froemmig
Füchtbauer
Fünfschilling
Furuta
Gagliardi
Galceran
Gale
Gamber
Gan
Gantz
Garcia
Garrels
Gelsen
Gersbach
Gertsenstein
Golovko
Gong
Baylor College of Medicine, Houston, USA
University of Manitoba, Winnipeg, Canada
BioTalentum Ltd, Godollo, Hungary
Ozgene Pty Ltd, Berlin, Germany
Universität Heidelberg, Heidelberg, Germany
GVG Genetic Monitoring GmbH, Leipzig, Germany
e Wellcome Trust, Sanger Institute, Cambridge, U.K.
Northwestern University, FSM, Chicago, USA
University of South Bohemia in Ceske Budejovice, České Budějovice, Czech Republic
Takeda Cambridge, Cambridge, U.K.
University of Cambridge, Cambridge, U.K.
INSERM DELEGATION PARIS 12, CRETEIL, France
NHLBI/NIH, Bethesda, USA
Université de Genève, Geneva 4, Switzerland
Wellcome Trust-MRC Stem Cell Institute, Cambridge, U.K.
Stowers Institute for Medical Research, Kansas City, USA
Universitätsklinikum Freiburg, Freiburg, Germany
Isfahan branch, IAU, Isfahan, Iran
University of Ottawa, Ottawa, Canada
Institut Curie Centre de Recherche, PARIS, France
Institute for Experimental Medicine, HAS, Budapest, Hungary
Translational Animal Research Center (TARC), Mainz, Germany
Genentech, Inc., South San Francisco, USA
Charles University in Prague, Prague, Czech Republic
Kurdistan University of Medical Sciences, Sanandaj, Iran
Oregon Health & Science University, Portland, OR, USA
University of Zurich, Zürich, Switzerland
MRC Harwell, Didcot, U.K.
Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain
Charles River Laboratories, Clion Park, USA
Max-Planck-Institute for molecular Genetics, Berlin, Germany
UC-Irvine (retired), Anaheim, USA
AXON Neuroscience SE, Bratislava, Slovak Republic
Veterinärmedizinische Universität Wien, Wien, Austria
Ciphe, Marseille Cedex 09, France
University College London, London, U.K.
Duke University, Durham, NC 27710, USA
Medicla College of Wisconsin, Milwaukee, USA
genOway, Lyon, France
IMG AS CR, Prague 4, Czech Republic
Institute for Research in Biomedicine, Barcelona, Spain
Hellenic Pasteur Institute, Athens, Greece
MRC-Harwell, Didcot, U.K.
Karolinska Institutet, Huddinge, Sweden
Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany
Central Institute of Mental Health, Mannheim, Germany
Aarhus University, Aarhus C, Denmark
MPI-EM, Göttingen, Germany
RIKEN CLST/CDB, Kobe, Japan
Regeneron Pharmaceuticals, Tarrytown, USA
Instituto de Neurociencias, San Juan de Alicante, Spain
Regeneron Pharmaceuticals. Inc, Tarrytown, USA
Horizon Discovery Ltd, Cambridge, U.K.
University of Rochester Medical Center, Rochester, USA
University of California, San Diego, La Jolla, USA
Institut Curie Centre de Recherche, Paris Cedex 05, France
Hannover Medical School, Hannover, Germany
GVG Genetic Monitoring, Leipzig, Germany
Duke University, Durham, USA
Toronto Centre for Phenogenomics, Toronto, Canada
Texas A&M Institute for Genomic Medicine, College Station, USA
Regeneron Pharmaceuticals, Tarrytown, USA
March 20–23, 2016, Prague, Czech Republic
Claudia
Carola
Celine
Klára
Evelyn
Veronika
Artiom
Mo
Frederic
Caiying
CB
Rebecca
Judy
John
F. Kent
Radmila
Sylva
Adele
Sarah
Jason
Karen
omas
Yann
Irm
Christopher
Joseph
Sigrid
Steff en
Peter
Tino
Sarah
Jan
Sandra
Maria
Ute
Martin
Magdalena
Chih Wei
Yueh-Chiang
Philip
Kerstin
Farah
Patricia
Neil
Elizabeth
Zuzana
Gulayse
Goo
Vera
Jean
Irena
Boris
Jana
Chengliu
Tjard
Sarah
Aurelie
Santiago
Colin
Alexandra
Cunxiang
Rainer
Gösele
Granberg
Granier
Grantz Šašková
Grau
Grešáková
Gruzdev
Guan
Guinut
Guo
Gurumurthy
Haff ner-Krausz
Hallett
Halupowski
Hamra
Hanečková
Haralambous
Harman
Hart-Johnson
Heaney
Helliwell
Hennek
Hérault
Hermans-Borgmeyer
Hess
Hickey
Hoff mann
Hoff mann
Hohenstein
Hochepied
Homann
Honetschläger
Hope
Hörnberg
Hostick
Hrabé de Angelis
Hryhorowicz
Hu
Hu
Hublitz
Huebner
Hughes
Humire
Humphreys
Hurd
Ileninova
Ince Dunn
Jang
Jansen
Jaubert
Jenickova
Jerchow
Ježková
Jin
Joerss
Johnson
Jory
Josa
Jouhanneau
Joyner
Ju
Kabisch
MAX-DELBRÜCK-CENTER, Berlin, Germany
Umeå University, Umeå, Sweden
INSERM, Toulouse, France
IOCB AS CR,v.v.i., Praha 6, Czech Republic
Wellcome Trust Sanger Institute, Hinxton, U.K.
IMG ASCR v.v.i., Prague 4, Czech Republic
NIEHS, Research Triangle Park, NC, USA
Medical Research Council Harwell, Didcot, U.K.
JANVIER LABS, Saint Berthevin Cedex, France
HHMI, Janelia Research Campus, Ashburn, VA, USA
UNMC, Omaha, USA
Weizmann Institute, Rehovot, Israel
Purdue University, West Lafayette, USA
Novartis Institutes of Biomedical Research, Cambridge, USA
Home, Lewisville, USA
Institute of Molecular Genetics, Prague, Czech Republic
Hellenic Pasteur Institute, Athens, Greece
Children‘s Hospital of Philadelphia, Philadelphia, USA
e Francis Crick Institute, London, U.K.
Baylor College of Medicine, Houston, USA
Birmingham University, Birmingham, U.K.
ETH Zürich, Zürich, Switzerland
PHENOMIN, Illkirch, France
ZMNH/UKE, Hamburg, Germany
University of Zurich, Zurich, Switzerland
Regeneron Pharmaceuticals Inc., Tarrytown, USA
University Heidelberg, Medical Faculty Mannheim, Mannheim, Germany
Max-Planck Institute of IE, Freiburg, Germany
e Roslin Institute, Midlothian, U.K.
Ghent University - VIB, Ghent, Belgium
ZMNH/UKE, Hamburg, Germany
Institute of Molecular Genetics of the ASCR,v.v.i., Prague 4, Czech Republic
Brigham Young University, Provo, USA
Umeå University, Umeå, Sweden
University of Oregon, Eugene, USA
Helmholtz Zentrum Muenchen GmbH, Neuherberg, Germany
Poznan University of Life Sciences, Poznan, Poland
Chang Gung University, Taoyuan, Taiwan
Cincinnati Children‘s Hospital Medical Center, Madeira, USA
University of Oxford, Oxford, U.K.
Experimental Tumorpathology, Erlangen, Germany
Beatson Institute for Cancer Reasearch, Glasgow, U.K.
Karolinska Institutet, Stockholm, Sweden
e University Of Manchester, Manchester, U.K.
University of Edinburgh, Edinburgh, U.K.
Institute of Molecular Genetics AS CR, v. v. i., Prague, Czech Republic
Koc University, Istanbul, Turkey
Seoul National University, Seoul, South Korea
caesar Research Center, Bonn, Germany
Institut Pasteur, Paris, France
Institute of Molecular Genetics, Prague, Czech Republic
Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
Institute of molecular genetics, Praha 4, Czech Republic
Georgia State University, Atlanta, USA
Leibniz Institute on Aging, Jena, Germany
Harvard University, Cambridge, USA
NCBS, GKVK PO, Bengaluru, India
Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain
Institut Curie Centre de Recherche, Paris Cedex 05, France
Sloan Kettering Institute, New York, USA
Model Animal Research Center of Nanjing University, Nanjing, China
MDC Berlin-Buch, Berlin, Germany
63
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
64
Shawn
Byeong Cheol
Benoît
Petr
Amy
Natalia
Liam
Margaret
Kevin
Janet
Bob
Zuzana
Sang Yong
Ruairidh
Slavomír
Arif
Maja
Hiroshi
Agata
Uwe
Jason
Ulrich
Regina
Martha
Jiří
Peter J
omas
Suman
Witold
Jana
Dana
Justyna
Evguenia
Katalin
Zbynek
Harald
Grzegorz
Kathy
Lona
Sonja
Sarah
Wieslaw
Gary
Ralf
Nicole
Wilfried
Peter
Satu
Irina
Jitka
Ka-Man Venus
Vladimir
Carlisle
Adrian
Francina
Denise
Nicola
Melissa
Anna
Anja
Ann
Marine
Kalloway
Kang
Kanzler
Kasparek
Kaucher
Kazakova
Keegan
Keighren
Kelley
Kenyon
Kesterson
Khorshidi
Kim
King
Kinský
Kisana
Kitic
Kiyonari
Klejman
Klemm
Klotz
Kloz
Kneuttinger
Koerner
Kohoutek
Koch
Kolbe
Komjeti
Konopka
Kopkanová
Kopperova
Kopycińska
Kouranova
Kovács
Kozmik
Kranz
Kreiner
Krentz
Kroese
Kropp
Krueger
Krzyzak
Kucera
Kühn
Kuepper
Kues
Kutny
Kuure
Lagutina
Lachova
Lai
Landa
Landel
Landsberger
Langa
Lanza
Laprano
Larson
Lastuvkova
Lau
Lawler
Le Clech
Medical College of Wisconsin, Waukesha, USA
Seoul National University Hospital, Seoul, South Korea
Max-Planck Institute of Immunobiology and Epigenet, Freiburg, Germany
Institute of Molecular Genetics, Prague, Czech Republic
Washington State University, Pullman, WA, USA
e Francis Crick Institute, London, U.K.
Masarykova univerzita, Brno, Czech Republic
University of Edinburgh, Edinburgh, U.K.
Icahn School of Medicine at Mount Sinai, New York, USA
MRC, Swindon, U.K.
University of Alabama at Birmingham, Birmingham, USA
Karolinska Institutet, Stockholm, Sweden
NYU Langone Medical Center, New York, USA
Medical Research Council, Mary Lyon Centre, Didcot, U.K.
BIOCEV, Vestec, Praha-Vychod, Czech Republic
Karolinska Institutet, Stockholm, Sweden
Universitätsmedizin der Johannes Gutenberg Univers, Mainz, Germany
RIKEN, Kobe, Japan
Nencki Institute of Experimental Biology, Warsaw, Poland
MPI for Infection Biology, Berlin, Germany
Medical College of Wisconsin, Campbellsport, USA
Deutsches Krebsforschungszentrum, Heidelberg, Germany
DZNE Munich, Munich, Germany
University of Edinburgh, Edinburgh, U.K.
Výzkumný ústav veterinárního lékařství, v. v. i., Brno, Czech Republic
University of Colorado School of Medicine, Aurora, USA
Veterinärmedizinische Universität Wien, Wien, Austria
European Molecular Biology Laboratory, Monterotondo, Italy
Nencki Institute of Experimental Biology, Warsaw, Poland
IMG CAS v.v.i., Praha 4, Czech Republic
UMG, Praha 4, Czech Republic
Institute of Molecular Genetics of the ASCR, Prague, Czech Republic
Horizon Discovery, Maryland Heights, USA
Institute for Experimental Medicine, Budapest, Hungary
Institute of Molecular Genetics, Praha 4, Czech Republic
Gene Bridges GmbH, Heidelberg, Germany
Institute of Pharmacology, PAS, Krakow, Poland
University of Wisconsin Madison, Madison, USA
e Netherlands Cancer Institute, Amsterdam, e Netherlands
Heinrich Heine University, Duesseldorf, Germany
University Hospital Jena, Jena, Germany
LIMES-GRC, Bonn, Germany
Duke Cancer Institute, Raleigh, USA
Max-Delbrück-Center for Molecular Medicine, Berlin, Germany
Heinrich Heine University, Duesseldorf, Germany
Friedrich-Löffler-Institut, Neustadt am Rübenberge, Germany
e Jackson Laboratory, Bar Harbor, USA
University of Helsinki, Laskut, Finland
Avantea, Cremona, Italy
Institute of Molecular Genetics of the ASCR, v. v., Praha 4, Czech Republic
Regeneron Pharmaceuticals, Inc., Tarrytown, USA
Institute of Physiology AS CR, Prague, Czech Republic
Muridae Scientific Consulting, Southwest Harbor, USA
Charité Universitätsmedizin Berlin, Berlin, Germany
Institut Pasteur, PARIS, France
Baylor College of Medicine, Houston, USA
e Beatson Institute for Cancer Research, Glasgow, U.K.
University of Kansas Medical Center, Kansas City, USA
EMBL, Monterotondo, Italy
Envigo RMS Srl, Bresso, Italy
Johns Hopkins Univ. School of Medicine, Baltimore, MD, USA
Roche Innovation Center Zuerich, Schlieren, Switzerland
March 20–23, 2016, Prague, Czech Republic
Angus
Ho
Jeff rey
Young Jae
Hong
ierry
Nicole
Adrian
Liangping
Margot
Daniel
Anne Karelia
Chengyu
Ke
Yong
Michela
Kent
Elsa
Teresa
Blanca
Anna
Robin
Pia
Jinping
Yonglun
Sophie
Rene
Esther
Lukasz
Péter
Katharine
Abdelkrim
William
Sabine
Suzy
Pina
Margarita
Javier
Isabel
Zoltan
Christin
Fukuyo
Elisabetta
Peter
Tanja
Sam
K. John
María Noel
Anne
Ylva
Jacek
Severine
Nina
Alexander
Joff rey
Geert
Alan
Soo Jin
Marta
Hiromi
Fujio
Francisco JM
Lee
Lee
Lee
Lee
Lei
Leluc
Lessner
Leu
Li
Linssen
Lipiński
Lipson Ruff ert
Liu
Liu
Liu
Lizier
Lloyd
Lopez
Lopez Rovira
Lopez-Biladeau
Lounkova
Lovell-Badge
Lundegaard
Luo
Luo
Lyst
Maehr
Mahabir-Brenner
Majewski
Major
Mankelow
Mannioui
Mansfield
Manz
Markossian
Marotta
Marques
Martin
Martin-Dorado
Mate
Matka
Matsushita Hedlund
Mattei
Matthews
Maurer
Mayo
McLaughlin
Meikle
Meinzinger
Mende
Mendrychowski
Menoret
Messner
Meyer
Mianne
Michel
Mileham
Min-Weißenhorn
Miret
Miura
Miyawaki
Mojica
UC Berkeley, Berkeley, USA
National Cancer Center, Goyang, South Korea
Regeneron Pharmaceuticals, Inc., Tarrytown, USA
Gachon University, Incheon, South Korea
Novartis Institutes for Biomedical Research, Cambridge, USA
JANVIER LABS, Saint-Berthevin Cedex, France
Eppendorf AG, Hamburg, Germany
University of Pennsylvania, Kennett Square, PA, USA
Sun Yat-sen University, Guangzhou, China
Leiden University Medical Center, Leiden, e Netherlands
Poznan University of Life Sciences, Poznan, Poland
CNRS, ORLEANS, France
NIH, Bethesda, MD, USA
University of Liverpool, Liverpool, U.K.
Aarhus University, Aarhus C, Denmark
IRGB UOS CNR, Milano, Italy
Mouse Biology Program, Davis, USA
MPI, Freiburg im Breisgau, Germany
Institute for Research in Biomedicine, Barcelona, Spain
Washington State University, Pullman, WA, USA
Institute of Molecular Genetics, ASCR, Prague 4, Czech Republic
e Francis Crick Institute, London, U.K.
University of Copenhagen, Copenhagen, Denmark
University of Utah, Salt Lake City, Utah, USA
Aarhus University, Aarhus, Denmark
University of Manchester, Manchester, U.K.
UMass Medical School, Worcester, USA
Center for Molecular Medicine, Cologne, Germany
Intern. Institute of Molecular and Cell Biology, Warsaw, Poland
NAIK, Gödöllő, Hungary
Francis Crick Institute, London, U.K.
UPMC, Paris, France
University of Cambridge, Cambridge, U.K.
MDC - Berlin, Berlin, Germany
ENS-IGFL-INRA, Lyon, France
Biogem, Ariano Irpino, Italy
Universidad de Leon, Leon, Spain
University of Copenhagen, Copenhagen, Denmark
Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain
Institute of Experimental Medicine, Budapest, Hungary
University of Heidelberg, Heidelberg, Germany
Karolinska Institutet, Stockholm, Sweden
National Research Council, Rome, Italy
Wellcome Trust Sanger Institute, Hinxton, U.K.
Universität des Saarlandes, Homburg, Germany
Mayo Seed Co, Knoxville TN, USA
Research Institute Nationwide Children‘s Hospital, Columbus, USA
Institut Pasteur de Montevideo, Montevideo, Uruguay
PolyGene AG, Rümlang, Switzerland
University of Cologne, Cologne, Germany
University of Edinburgh, Edinburgh, U.K.
Association ITERTUN, Nantes, France
University of Turku, Turku, Finland
MDC, Berlin, Germany
Medical Research Council Harwell, Didcot, U.K.
Charité-Universitätsmedizin Berlin, Berlin, Germany
Genus plc, DeForest, USA
Max-Planck Institute for Biochemistry & Neurobi, Martinsried, Germany
e Francis Crick Institute, London, U.K.
Tokai University, Isehara, Japan
Tokyo Denki University, Saitama, Japan
University of Alicante, Alicante, Spain
65
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
66
Natalia
Lluis
Sara
Pedro
Iva
Christian
Mirjam
Jaime
Stephen
Martin
Andras
Ronald
Annette
Stuart
Erik
Rada
Emily
Timothy
Ana
Agnieszka
Nathan
Lauryl
Jon
Melissa
Goo Taeg
Masato
Gabriela
Ramiro
Heide
Jana
Sagrario
Sara
Oskar
Henrieta
Jong Hoon
Jan
Carlos
Sara
Mercier
Bastian
Guillaume
Mirjam
Pawel
Monica
Sima
Andrea
Trine Skov
Yvonne
Kevin
Klaus
Amanda
C. Oscar
Karolina
Lucas
Anne
Immacolata
Sandra
Sara
Michal
Chris
Prem
Christopher
Moncaut
Montoliu
Morando
Moreira
Morse
Mosimann
Müller
Muñoz
Murray
Musil
Nagy
Naumann
Nebenius-Handreck
Newman
Nilsson
Norinsky
Normand
Nottoli
Nóvoa
Nowak
Nowak
Nutter
Oatley
Oatley
Oh
Ohtsuka
Olech
Olivera
Oller
Oltova
Ortega
Ortica Gatti
Ortiz
Pálešová
Park
Parker- ornburg
Parras
Parsa
Pascale
Pasche
Pavlovic
Peetz
Pelczar
Pereira
Peretz
Perota
Petersen
Petersen
Peterson
Pfeff er
Pickard
Pintado
Piotrowska-Nitsche
Pitt
Plück
Porreca
Potyšová
Pozzi
Pravenec
Preece
Premsrirut
Proudfoot
Cancer Research UK Manchester Institute, Manchester, U.K.
Centro Nacional de Biotecnologia (CNB-CSIC), Madrid, Spain
Fondazione Istituto Italiano di Tecnologia, Genova, Italy
EMBL, Monterotondo, Italy
Charles River Labs, Wilmington, USA
University of Zürich, Zürich, Switzerland
Unversität des Saarlandes, Homburg, Germany
Fundacion CNIO Carlos III, Madrid, Spain
e Jackson Laboratory, Bar Harbor, USA
TECNIPLAST SPA, Buguggiate (Va), Italy
Mount Sinai Hospital, Toronto, Canada
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
University of Basel, Basel, Switzerland
New Hunts House, Kings College London, London, U.K.
Karolinska Institutet, Stockholm, Sweden
e Rockefeller University, New York, USA
LFB BIOTECHNOLOGIES, Courtaboeuf, France
Yale School of Medicine, New Haven, USA
Fundação Calouste Gulbenkian Instituto Gulbenkian, Oeiras, Portugal
Poznan University of Life Sciences, Poznan, Poland
Transnetyx, Cordova, USA
e Hospital for Sick Children, Toronto, Canada
Washington State University, Pullman, WA, USA
Washington State University, Pullman, USA
Ewha Womans University, Seoul, South Korea
School of Medicine, Tokai University, Isehara, Japan
Nencki Instytute of Experimental Biology, Warsaw, Poland
Kheiron SA, Caba, Argentina
University of Basel, Basel, Switzerland
Institute of Molecular Genetics, Prague 4, Czech Republic
Fundacion CNIO Carlos III, Madrid, Spain
Paris-Saclay Institute for Neuroscience (Neuro-PSI, Gif-sur-Yvette, France
Helmholtz Zentrum Muenchen, Neuherberg, Germany
Ústav molekulární genetiky AV ČR, v. v. i., Praha, Czech Republic
Sookmyung Women‘s University, Seoul, South Korea
ISTT, Friendswood, Texas, USA
Inserm, PARIS, France
medical university of kurdistan, sanandaj, Iran
CNRS, Toulouse, France
Helmholtz Centre for Infection Research, Braunschweig, Germany
CERMB-GIE, ILLKIRCH, France
Max-Planck-Institute for molecular Genetics, Berlin, Germany
University of Basel, Basel, Switzerland
Toronto Centre for Phenogenomics, Toronto, Canada
Weizmann Institute of Science, Rehovot, Israel
Avantea, Cremona, Italy
Aarhus University, Aarhus C, Denmark
EMBL Heidelberg, Heidelberg, Germany
e Jackson Laboratpry, Bar Harbor, USA
Heinrich-Heine-University, Düsseldorf, Germany
University of Leicester, Leicester, U.K.
University of Seville, Espartinas (Sevilla), Spain
Emory University, Atlanta, USA
Malaghan Institute of Medical Research, Wellington, New Zealand
Uni Köln, Köln, Germany
Biogem S.c.ar.l., Ariano Irpino, Italy
Institute of molecular genetics, Praha 4, Czech Republic
UCL Institute of Child Health, London, U.K.
Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
University of Oxford, , U.K.
Mirimus Inc., Woodbury, USA
Roslin Institute, Midlothian, U.K.
March 20–23, 2016, Prague, Czech Republic
Feng-Lin
Anna
Jaya
Corinne
Vanessa
Xin
Mohamed
Carla
Wendy
Martina
Severine
Hermann
Luca
Alex
Susan
Isabel
Peter
Merone
Giorgio
Paolo
Ian
Pablo
Rene
omas
Filomena
Nicola Antonino
Edward
Manuel
Monica
Mara
Kristin
Masahiro
om
Jim
Denise
Aaron
Gregory
John Richard
Radislav
Stefan
JeKyung
Konstantin
Matthew
Jacqueline
Doron
Michele
Charlotte
John
Kai
David
Lisanne
Dagmar
Bjoern
Patrizia
Sabine
Kirsten
Carlos Daniel
Maj
Petra
Monika
Britt-Mari
Jackie
Pu
Pujol
Purushotham
Quadalti
Quillaud-Chenouard
Rairdan
Rajab
Reale
Reinemann
Reiss
Remy
Riedesel
Roberto
Robertson
Rodger
Rollán
Romanienko
Roose
Rosati
Rosellini Tognetti
Rosewell
Ross
Rudat
Ruelicke
Russo
Russo
Ryder
Sanchez-Martin
Sanchez-Ruiz
Sannai
Sapp
Sato
Saunders
Sawitzke
Scotland
Scott
Scott
Seavitt
Sedlacek
Selbert
Seong
Severinov
Sharp
Sharpe
Shmerling
Schaff ner
Schick
Schimenti
Schönig
Schreiber
Schulze
Schumacher
Schuster
Schütz
Schwarz
Sigrist
Silva
Simonsen Jackson
Sipilä
Sivá
Skog
Sloane Stanley
National Taiwan University, Taipei, Taiwan
Universitat Autònoma de Barcelona, Bellaterra, Spain
NCBS(National Center for Biological Sciences), Bangalore, India
AVANTEA srl, Cremona, Italy
INSERM UMR 1064/Platform TRIP, NANTES, France
Genentech Inc., South San Francisco, USA
King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia
Biogem s.c.a.r.l., Ariano Irpino (AV), Italy
e Jackson Laboratory, Bar Harbor, USA
Gene Bridges GmbH, Heidelberg, Germany
Association ITERTUN, Nantes, France
University Medical Center Göttingen, Göttingen, Germany
Biogem S.c.ar.l., Ariano Irpino (AV), Italy
Roslin Institute, Midlothian, U.K.
Medical Research Council (Harwell), Didcot, U.K.
Fundación CNIC, Madrid, Spain
Memorial Sloan Kettering Cancer Center, New York, USA
Genentech, South San Francisco, USA
TECNIPLAST SPA, Buguggiate (Va), Italy
DRFZ, Berlin, Germany
Francis Crick Institute, South Mimms, U.K.
University of California Davis, Davis, USA
OVGU Magdeburg, Magdeburg, Germany
Veterinärmedizinische Universität Wien, Wien, Austria
biogem scarl, ariano irpino (AV), Italy
Biogem S.c.ar.l., Ariano Irpino (AV), Italy
Wellcome Trust Sanger Institute, Cambridge, U.K.
University of Salamanca, Salamanca, Spain
Uniklinik Koeln, Cologne, Germany
Leibniz Institute on Aging, Jena, Germany
LFB USA, Worcester, USA
Section of Gene Expression Regulation, Frontier Sc, Kagoshima, Japan
Home Address, Ann Arbor, Michigan, USA
EMBL-Monterotondo, Monterotondo, Italy
University of Cambridge, Cambridge, U.K.
University of Exeter, Exeter, U.K.
NIEHS, Research Triangle Park, NC, USA
Baylor College of Medicine, Houston, USA
Institute of Molecular Genetics of the ASCR, v. v., Prague, Czech Republic
PolyGene AG, Rümlang, Switzerland
KMPC/SNU, Seoul, South Korea
Skolkovo Institute of Science and Technology, Moscow, Russia
e University of Edinburgh, Edinburgh, U.K.
Weatherall Institue Of Molecular Medicine, Oxford, U.K.
PolyGene AG, Rümlang, Switzerland
ETH Zürich, Zürich, Switzerland
Charite Universitätsmedizin Berlin, Berlin, Germany
Cornell University, Ithaca, USA
Central Institute of Mental Health, Mannheim, Germany
MDC Berlin-Buch, Berlin, Germany
Charité Berlin, Berlin, Germany
Universität Heidelberg, Heidelberg, Germany
Institute of Molecular Genetics, Prague, Czech Republic
Universität Heidelberg, Heidelberg, Germany
Springer, Heidelberg, Germany
Brown University, Providence, USA
Faculty of Medicine of the University of Porto, Porto, Portugal
e University of Manchester, Manchester, U.K.
University of Turku, Turku, Finland
IOCB AS CR,v.v.i., Prague, Czech Republic
Karolinska Institutet, Huddinge, Sweden
Westherall Institute of Molecular Medicine, Oxford, U.K.
67
13TH TRANSGENIC TECHNOLOGY MEETING
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
68
Ryszard
Mike
Miguel
Scott
Raija
Stephan
Tania
Jean-François
John
Aimee
Didier
Malgorzata
Sara
David
Michelle
Sandra
Barbara
Douglas
Georgios
Xin
Gábor
Marlena
Anna
Norihiro
Robert
Toru
Susan
Elly
Adrianne
Ali Cihan
Laura
Lydia
Stephan
Marie
Laurent
Andreas
H.-Christian
Julie
Melita
Cecilia Soledad
Barbara
Ailsa
Ching-Yen
Daniel
Claire
Bianca
Franciscus
Nanda
Marian
Pierfrancesco
Sunita
Kuzhalini
Sonia
Anna
Gabriel
Guglielmo
Ingo
Monika
omas
Barbora
Reetta
Rudolf
Słomski
Snaith
Soares
Soderling
Soininen
Sonntag
Sorg-Guss
Spetz
Spijkers
Stablewski
Stainier
Statkiewicz
Steinmann
Stevenson
Stewart
Stobrawa
Stone
Strathdee
Strimpakos
Su
Szabó
Szalata
Szydlowska
Tada
Ta
Takeo
Tamowski
Tanaka
Tasdemir
Taskin
Tatangelo
Teboul
Teglund
Teixeira
Tesson
Teubner
eussl
omson
Ticevic
Tomni
Tondelli
Travers
Tsai
Turnbull
Usal
Van Bree
van der Hoeven
van Eeken
van Roon
Vargiu
Varsani-Brown
Vasudevan
Verp
Veselova
Vichera
Vismara
Voigt
Volckova
Volz
Vorlova
Vuolteenaho
Walczak
Institute of Human Genetics,Polish Academy of Sci., Poznan, Poland
MedImmune, Cambridge, U.K.
Faculty of Medicine of the University of Porto, Porto, Portugal
Duke University, Durham, USA
University of Oulu, Oulu, Finland
PolyGene AG, Rümlang, Switzerland
CERMB-GIE, Illkirch, France
Friedrich Miescher Institute, Basel, Switzerland
Academic Medical Center, Amsterdam, e Netherlands
Roswell Park Cancer Institute, Buff alo, USA
Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany
Maria Sklodowska-Curie Memorial Cancer Center and, Warsaw, Poland
Institute of Pathology, Erlangen, Germany
Beatson Institute for Cancer Research, Glasgow, U.K.
Medical Research Council, Didcot, U.K.
Eppendorf AG, Hamburg, Germany
ParaTechs Corp, Lexington, USA
CRUK Beatson Institute, Glasgow, U.K.
CNR, Rome, Italy
HHMI, Janelia Research Campus, Ashburn, VA, USA
Institute of Experimental Medicine, Budapest, Hungary
Poznan University of Life Sciences, Poznan, Poland
Institute of Molecular Biotechnology, Wien, Austria
Juntendo University Graduate School of Medicine, Tokyo, Japan
e Jackson Laboratory, Bar Harbor, USA
Kumamoto University, Kumamoto, Japan
U of Utah Transgenic Core, Salt Lake City, USA
Technische Universitaet Dresden, Dresden, Germany
Helmholtz Zentrum Muenchen, Neuherberg, Germany
Koç Üniversity, Istanbul, Turkey
allevamenti plaisant srl, rome, Italy
Medical Research Council, Swindon, U.K.
Karolinska Institutet, Stockholm, Sweden
ENS-Lyon/PBES, Lyon, France
Association ITERTUN, Nantes, France
Medical Faculty of RWTH Aachen, Aachen, Germany
IMP/Research Institute of Mol. Pathology, Vienna, Austria
University of Edinburgh, Edinburgh, U.K.
IMBA - Institute of molecular biotechnology, Vienna, Austria
Friedrich-Schiller-Universität Jena, Jena, Germany
Institute for Research in Medicine, Barcelona, Spain
Univeristy of Edinburgh, Edinburgh, U.K.
Transgenic Core Facility, Taipei, Taiwan
New York University School of Medicine, New York, NY, USA
INSERM UMR 1064/Platform TRIP, Nantes, France
Pharming group N.V., Evry Cedex, France
German Cancer Research Center, Heidelberg, Germany
Academic Medical Center, Amsterdam, e Netherlands
InnoSer, Lelystad, e Netherlands
Fundacion CNIO Carlos III, Madridç, Spain
Francis Crick institute, London, U.K.
Priority One Services, Inc, Alexandria, USA
EPFL SV CPG-GE, Lausanne, Switzerland
BIOCAD, Saint-Petesburg, Strelna, Russia
Kheiron SA, Caba, Argentina
TECNIPLAST SPA, Buguggiate (Va), Italy
Max Planck Institute for Biology of Aging, Cologne, Germany
IMG, Prague, Czech Republic
Charles River, Wilmington, Boston, USA
Ústav organické chemie a biochemie AV ČR, v.v.i., Praha 6, Czech Republic
University of Oulu, Oulu, Finland
Eppendorf AG, Hamburg, Germany
March 20–23, 2016, Prague, Czech Republic
Haoyi
Yong
Soren
Xavier
Fiona
Saiphone
Gabbine
Benedikt
Linda
Hartmut
Kerstin
Ron
Marie-Louise
Frank
Bruce
Elizabeth
Mark
Steve
Jolene
Dagmar
John
Lars
Jacek Rodegar
Sophie
Po-Yuen
Wen AI
Xiaoli
Wang
Huirong
Xiaoling
Xiaojun
Wenhao
Selin
Chih-Hsiang
Ghassan
Su-Cheong
Ming
I-Shing
Soo-Young
Hong
Bernd
Branko
Fuping
Junli
Shuqin
Yan
Fei
Tomas
Anne
Ilona
Wang
Wang
Warming
Warot
Warrander
Webb
Wee
Wefers
Wei
Weiler
Weisheit
Weiss
Werner
Wesarg
Whitelaw
Williams
Willington
Wilson
Windle
Wirth
Wiseman
Wittler
Wojciechowski
Wood
Wu
Wu
Wu
Xiaomin
Xie
Xie
Xing
Xu
Yagcioglu
Yang
Yehia
Yeom
Yin
Yu
Yum
Zeng
Zetsche
Zevnik
Zhang
Zhang
Zhang
Zhou
Zhu
Zikmund
Zintzsch
Zvetkova
Institute of Zoology, Chinese Academy of Sciences, Beijing, China
ird Military Medical University, Chongqing, China
Genentech, Inc., South San Francisco, USA
EPFL SV CPG-GE, Lausanne, Switzerland
Beatson Institute for Cancer Research, Glasgow, U.K.
University of Colorado, Aurora, USA
Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, South Korea
DZNE Munich, Munich, Germany
Lund University, Lund, Sweden
BloodCenter of Wisconsin, Milwaukee, USA
Universitätsklinik Köln, Köln, Germany
Massachusetts Institute of Technology, Cambridge, USA
Karolinska Institutet, Stockholm, Sweden
Max-Planck-Institut for biology of aging, cologne, Germany
Roslin Institute, Midlothian, U.K.
University of Qld, Brisbane, Australia
Cancer Research UK, Manchester, U.K.
GlaxoSmithKline, Stevenage, U.K.
Virginia Commonwealth Univ, Richmond, USA
Helmholtz Centre for Infection Research, Braunschweig, Germany
AstraZeneca, Mölndal, Sweden
Max-Planck-Institute for Molecular Genetics, Berlin, Germany
IMBA, Vienna, Austria
e Francis Crick Institute, London NW7 1AA, U.K.
TMMC, Taipei, Taiwan
Chang Gung University, Taoyuan, Taiwan
University of Manitoba, Winnipeg, Canada
Model Animal Research Center of Nanjing University, Nanjing, China
Michigan State University, East Lansing, USA
University of Rochester Medical Center, Rochester, USA
yale university, New Haven, USA
University of Virginia, Charlottesville, VA, USA
Istanbul University Faculty of Veterinary Medicine, Istanbul, Turkey
Max Planck Institute of Immunology and Epigenetics, Freiburg, Germany
Rutgers University, Newark, USA
Seoul national university, Pyeongchang, South Korea
Peking University, Beijing, China
National Taiwan University College of Medicine, Taipei, Taiwan
622-85, Seoul National University, Seoul, South Korea
Stanford University, Stanford, USA
Broad Institute, Cambridge, USA
University of Cologne, Cologne, Germany
University of Turku, Turku, Finland
Gladstone Institutes, San Francisco, USA
HHMI, Janelia Research Campus, Ashburn, VA, USA
Aarhus University, Aarhus C, Denmark
University College London, London, U.K.
1. LF UK Praha, Praha 2, Czech Republic
Max Delbrück Center for Molecular Medicine, Berlin, Germany
University of Cambridge, Cambridge, U.K.
69
13TH TRANSGENIC TECHNOLOGY MEETING
March 20–23, 2016, Prague, Czech Republic
1
MAP OF PRAGUE
The Czech Centre for Phenogenomics
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