Experimental conditions on 11/6/2008
Phosphatases
New Assay
phosphatase PP1alpha
Catalog Ref:
751-1a
Price:
87 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant (E. coli )
Substrate:
DIFMUP (100 µM)
Measured product:
DIFMU
Incubation:
30 min./22°C
Method of Detection:
Fluorimetry
Reference:
LR-microcystin
(IC50: 170 nM)
Others:
cantharidin
endothall
HONKANEN, R.E., ZWILLER, J., MOORE, R.E., DAILY, S.L., KHATRA, B.S., DUKELOW, M. and BOYNTON, A.L. (1990)
J. Biol. Chem., 265: 19401-19404.
Phosphatases
New Assay
phosphatase VHR (DUSP3)
Catalog Ref:
751-vhr
Price:
87 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
DIFMUP (100 µM)
Measured product:
DIFMU
Incubation:
10 min./22°C
Method of Detection:
Fluorimetry
Reference:
ammonium molybdate
(IC50: 11500 nM)
Others:
RK-682
Na3VO4
DENU, J.M. and DIXON, J.E. (1995)
Proc. Nat. Acad. Sci. USA.., 92: 5910-5914.
Experimental conditions on 11/13/2008
Phosphatases
New Assay
phosphatase CD45
Catalog Ref:
751-ch
Price:
87 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant (yeast)
Substrate:
DIFMUP (100 µM)
Measured product:
DIFMU
Incubation:
10 min./22°C
Method of Detection:
Fluorimetry
Reference:
ammonium molybdate
(IC50: 60 nM)
Others:
Na3VO4
NSC 87877
WELTE, S., BARINGHAUS, K.H., SCHMIDER, W., MULLER, G., PETRY, S., TENNAGELS, N. (2005)
Anal. Biochem., 338: 32-38.
Experimental conditions on 11/6/2008
Phosphodiesterases
New Assay
PDE3B
Catalog Ref:
752-3b
Price:
49 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant (Sf9 cells)
Substrate:
cAMP (40 nM)
Measured product:
residual cAMP
Incubation:
30 min./22°C
Method of Detection:
HTRF
Reference:
milrinone
(IC50: 510 nM)
Others:
trequinsin
rolipram
BENDER, A.T. and BEAVO, J.A. (2006)
Pharmacol. Rev., 58: 488-520.
Experimental conditions on 11/24/2008
Prostanoid
New Assay
DP
Catalog Ref:
758-85a/85b
Price:
60 € /cpd
Standard turnaroud time:
3 weeks
Source:
human recombinant (CHO cells)
Measured product:
cAMP
Incubation:
20 min./22°C
Method of Detection:
HTRF
Agonist effect
Control: 1 µM BW 245C
References:
BW 245C (EC50: 2.4 nM)
PGF2α
U44069
PGD2
Antagonist effect
Stimulant: 30 nM BW 245C
References:
AH 6809 (IC50: 4300 nM)
BW A868C
AL 8810
AH 23848
WRIGHT, D.H., METTERS, K.M., ABRAMOVITZ, M. and FORD-HUTCHINSON, A.W. (1998)
Br. J. Pharmacol., 123: 1317-1324.
Prokineticin
New Assay
PK1
Catalog Ref:
722-79a/79b
Price:
60 € /cpd
Standard turnaroud time:
3 weeks
Source:
human recombinant (HEK-293 cells)
Measured product:
intracellular [Ca2+]
Incubation:
22°C
Method of Detection:
Fluorimetry
Agonist effect
Control: 30 nM PK1
References:
PK1 (EC50: 0.82 nM)
PK2
Antagonist effect
Stimulant: 3 nM PK1
References:
unavailable
CHEN, J., KUEI, C., SUTTON, S., WILSON,S., YU, J., KAMME, F., MAZUR, C., LOVENBERG, T., and LIU, C. (2005)
Mol. Pharmacol., 67: 2070-2076.
Prokineticin
New Assay
PK2
Catalog Ref:
722-80a/80b
Price:
60 € /cpd
Standard turnaroud time:
3 weeks
Source:
human recombinant (HEK-293 cells)
Measured product:
intracellular [Ca2+]
Incubation:
22°C
Method of Detection:
Fluorimetry
Agonist effect
Control: 10 nM PK2
References:
PK2 (EC50: 1.1 nM)
PK1
Antagonist effect
Stimulant: 2 nM PK2
References:
unavailable
CHEN, J., KUEI, C., SUTTON, S., WILSON,S., YU, J., KAMME, F., MAZUR, C., LOVENBERG, T., and LIU, C. (2005)
Mol. Pharmacol., 67: 2070-2076.
Experimental conditions on 11/24/2008
RTK
New Assay
Mer kinase
Catalog Ref:
768-mer
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight Poly GT [EY(4:1)]n (50 nM)
Measured product:
phospho-Ulight Poly GT [EY(4:1)]n
Incubation:
60 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 1.9 nM)
Others:
PP2
AG538
CAMENISCH, T.D., KOLLER, B.H., EARP, H.S. and MATSUSHIMA, G.K. (1999)
J. Immunol., 162: 3498-3503.
CTK
New Assay
CTK
Catalog Ref:
781-ctk
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight PolyGAT (5 nM)
Measured product:
phospho-Ulight PolyGAT
Incubation:
5 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 270 nM)
Others:
AG538
WOOD, A., SHUKLA, A., SCHNEIDER, J., LEE, J.S., STANTON, J.D., DZUIBA, T., SWANSON, S.K., FLORENS, L., WASHBURN, M.P.,
WYRICK, J., BHAUMIK, S.R. and SHILATIFARD, A. (2007)
Mol. Cell. Biol., 27: 709-720.
CTK
New Assay
Fer kinase
Catalog Ref:
768-fer
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight PolyGAT (5 nM)
Measured product:
phospho-Ulight PolyGAT
Incubation:
10 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine (IC50: 4.1 nM)
KIM, L. and WONG, T.W. (1998)
J. Biol. Chem., 273: 23542-23548.
CTK
New Assay
Srm kinase
Catalog Ref:
781-srm
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight PolyGAT (5 nM)
Measured product:
phospho-Ulight PolyGAT
Incubation:
10 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 625 nM)
Others:
PP1
PP2
KOHMURA, N., YAGI, T., TOMOOKA, Y., OYANAGI, M., KOMINAMI, R., TAKEDA, N., CHIBA, J., IKAWA, Y. and AIZAWA, S.
(1994)
Mol. Cell. Biol., 14: 6915-6925.
CMGC
New Assay
CDK3 (cycE1)
Catalog Ref:
781-cd3e
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + CFFKNIVTPRTPPPSQGK-amide (100 nM)
Measured product:
phospho-CFFKNIVTPRTPPPSQGK-amide
Incubation:
30 min./22°C
Method of Detection:
LANCE
Reference:
Ro-318220
(IC50: 5.65 nM)
Others:
staurosporine
roscovitine
olomoucine
BRAUN, K., HÖLZL, G., SOUCEK, T., GEISEN, C., MÖRÖY, T. and HENGSTSCHLÄGER, M. (1998)
Oncogene, 17: 2259-2269.
CMGC
New Assay
ERK5 (MAPK7)
Catalog Ref:
781-ERK5
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + CFFKNIVTPRTPPPSQGK-amide (100 nM)
Measured product:
phospho-CFFKNIVTPRTPPPSQGK-amide
Incubation:
90 min./22°C
Method of Detection:
LANCE
Reference:
Ro-318220
(IC50: 40 nM)
Others:
staurosporine
H-89
MODY, N., CAMPBELL, D.G., MORRICE, N., PEGGIE, M. and COHEN, P. (2003)
Biochem. J., 372: 567-575.
Other kinases
New Assay
PEK (EIF2AK3)
Catalog Ref:
708-pek
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + FLGFTYVAP (40 nM)
Measured product:
phospho-FLGFTYVAP
Incubation:
60 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 1020 nM)
Others:
Ro-318220
WEK, R.C., JIANG, H.-Y. and ANTHONY, T.G. (2006)
Bioch. Soc. Trans., 34: part 1.
Other kinases
New Assay
PLK1
Catalog Ref:
708-plk1
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + FLGFTYVAP (40 nM)
Measured product:
phospho-FLGFTYVAP
Incubation:
60 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 305 nM)
GOLSTEYN, R.M., MUNDT, K.E., FRY, A.M. and NIGG, E.A. (1995)
J. Cell. Biol., 129: 1617-1628.
CaMK
New Assay
TSSK2 (STK22B)
Catalog Ref:
742-tss2
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant (insect cells)
Substrate:
ATP + Ulight PLK-RRRSLLE (50 nM)
Measured product:
phospho-Ulight PLK-RRRSLLE
Incubation:
60 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 6.3 nM)
Others:
K252a
Raf-1 inhibitor
Ro-318220
HAO, Z., JHA, K.N., KIM, Y.H., VEMUGANTI, S., WESTBROOK, V.A., CHERTIHIN, O., MARKGRAF, K., FLICKINGER, C.J.,
COPPOLA, M., HERR, J.C. and VISCONTI, P.E. (2004)
Mol. Hum. Reprod., 10: 433-444.
AGC
New Assay
CRIK
Catalog Ref:
706-crik
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + ARTKQTARKSTGGKAPRKQLAGCG (75 nM)
Measured product:
phospho-ARTKQTARKSTGGKAPRKQLAGCG
Incubation:
30 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 40 nM)
Others:
Ro-318220
Bis 10
Y27632
ZHAO, Z.S. and MANSER, E. (2005)
Biochem. J., 386: 201-214.
AGC
New Assay
MRCKalpha
Catalog Ref:
743-mrcka
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + ARTKQTARKSTGGKAPRKQLAGCG (75 nM)
Measured product:
phospho-ARTKQTARKSTGGKAPRKQLAGCG
Incubation:
30 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 7.1 nM)
Others:
Ro-318220
K252a
Bis 10
TAN, I., SEOW, K.T., LIM, L. and LEUNG, T. (2001)
Mol. Cell. Biol., 21: 2767-2778.
AGC
New Assay
RSK3
Catalog Ref:
742-rsk3
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight PLK-RRRSLLE (50 nM)
Measured product:
phospho-Ulight PLK-RRRSLLE
Incubation:
60 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 2.25 nM)
Others:
Ro-318220
H-89
CHEN, R.H., SARNECKI, C. and BLENIS, J. (1992)
Mol. Cell. Biol., 12: 915-927.
TKL
New Assay
IRAK1
Catalog Ref:
749-ira1
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant (insect cells)
Substrate:
ATP + FLGFTYVAP (200 nM)
Measured product:
phospho-FLGFTYVAP
Incubation:
60 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 43.5 nM)
Others:
H-89
rottlerin
HUANG, Y., LI, T., SANE, D.C. and LI, L. (2004)
J. Biol. Chem., 279: 51697-51703.
TKL
New Assay
MLK2 (MAP3K10)
Catalog Ref:
768-mlk2
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant (insect cells)
Substrate:
ATP + ARTKQTARKSTGGKAPRKQLAGCG (25 nM)
Measured product:
phospho-ARTKQTARKSTGGKAPRKQLAGCG
Incubation:
120 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 79 nM)
Others:
Ro-318220
K252a
Bis 10
LIU, Y.F., DOROW, D. and MARSHALL, J. (2000)
J. Biol. Chem., 275: 19035-19040.
Experimental conditions on 12/4/2008
RTK
New Assay
ALK
Catalog Ref:
768-alk
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight-IRS-1 CKKSRGDYMTMQIG (150 nM)
Measured product:
phospho-Ulight-IRS-1 CKKSRGDYMTMQIG
Incubation:
60 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 3.9 nM)
Others:
K252a
AG1478
AG538
MOTEGI, A., FUJIMOTO, J., KOTANI, M., SAKURABA, H. and YAMAMOTO, T.
(2004)
J. Cell Sc., 117: 3319-3329.
RTK
New Assay
EphB1 kinase
Catalog Ref:
768-EB1
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight Poly GT [EY(4:1)]n (50 nM)
Measured product:
phospho-Ulight Poly GT [EY(4:1)]n
Incubation:
30 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 160 nM)
Others:
PP1
PP2
VINDIS, C., CERRETTI, D.P., DANIEL, T.O. and HUYNH-DO, U. (2003)
J. Cell. Biol., 162: 661-671.
RTK
New Assay
EphA2 kinase
Catalog Ref:
768-EA2
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight Poly GT [EY(4:1)]n (50 nM)
Measured product:
phospho-Ulight Poly GT [EY(4:1)]n
Incubation:
30 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 80.5 nM)
Others:
PP1
PP2
PD153035
KINCH, M.S., MOORE, M.B. and HARPOLE, D.H. Jr. (2003)
Clin. Cancer Res., 9: 613-618.
RTK
New Assay
ROR2 kinase
Catalog Ref:
768-ror2
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight Poly GT [EY(4:1)]n (50 nM)
Measured product:
phospho-Ulight Poly GT [EY(4:1)]n
Incubation:
120 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 1.7 nM)
Others:
AG1478
AG538
NISHITA, M., YOO, S.K., NOMACHI, A., KANI, S., SOUGAWA, N., OHTA, Y., TAKADA, S., KIKUCHI, A. and MINAMI, Y. (2006)
J. Cell Biol., 179: 555-562.
RTK
New Assay
TRKC
Catalog Ref:
768-TrkC
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight Poly GT [EY(4:1)]n (50 nM)
Measured product:
phospho-Ulight Poly GT [EY(4:1)]n
Incubation:
15 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 1.65 nM)
Others:
PP2
AG1478
K252a
ROBINSON, L.L., TOWNSEND, J. and ANDERSON, R.A. (2003)
J. Clin. Endocrinol. Metab., 88: 3943-3951.
CTK
New Assay
Ack
Catalog Ref:
781-ack
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight Poly GT [EY(4:1)]n (50 nM)
Measured product:
phospho-Ulight Poly GT [EY(4:1)]n
Incubation:
60 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 4.9 nM)
Others:
PP2
AG1478
AG538
MAHAJAN, N.P., WHANG, Y.E., MOHLER, J.L. and EARP H.S. (2005)
Cancer Res., 65: 10514-10523.
Other kinases
New Assay
WNK3
Catalog Ref:
768-wnk3
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + ARTKQTARKSTGGKAPRKQLAGCG (75 nM)
Measured product:
phospho-ARTKQTARKSTGGKAPRKQLAGCG
Incubation:
30 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 3150 nM)
Others:
Ro-318220
K252a
rottlerin
RINEHART, J., KAHLE, K.T., DE LOS HEROS, P., VAZQUEZ, N., MEADE, P., WILSON, F.H., HEBERT, S.C., GIMENEZ, I., GAMBA,
G. and LIFTON, R.P. (2005)
Proc. Natl. Acad. Sci. U S A., 102: 16777-16782.
AGC
New Assay
PKN1
Catalog Ref:
707-pkn1
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + CREBtide (CKRREILSRRPSYRK) (25 nM)
Measured product:
phospho-CREBtide (CKRREILSRRPSYRK)
Incubation:
15 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 2 nM)
Others:
Ro-318220
H-89
LARTEY, J., SMITH, M., PAWADE, J., STRACHAN, B., MELLOR, H. and LOPEZ BERNAL, A. (2007)
Biol. Reprod., 76: 971-982.
TKL
New Assay
LRRK2
Catalog Ref:
768-lrrk2
Price:
20 € /well
Standard turnaroud time:
3 weeks
Source:
human recombinant
Substrate:
ATP + Ulight PLK-RRRSLLE (50 nM)
Measured product:
phospho-Ulight PLK-RRRSLLE
Incubation:
30 min./22°C
Method of Detection:
LANCE
Reference:
staurosporine
(IC50: 1.95 nM)
Others:
H-89
Ro-318220
K252a
GREGGIO, E., ZAMBRANO, I., KAGANOVICH, A., BEILINA, A., TAYMANS, J.M., DANIËLS, V., LEWIS, P., JAIN, S., DING, J.,
SYED, A., THOMAS, K.J., BAEKELANDT, V. and COOKSON, M.R. (2008)
J. Biol. Chem., 283: 16906-16914.