Product Information Sheet
Order: # AC-101-JB
Immobilized gamma-Aminophenyl-ATP (C10-spacer), Syringe column - Agarose
SUMMARY
shipped on blue ice; store at 4 °C
45-165 µm
Toyopearl AF- 650M
characteristics
40-90 µm (mean pore size: 1000 Å)
11.5 cm/h
60-600 cm/h
Agarose characteristics
Bead/Particle size
Recommended linear flow
rate
Maximum pressure
pH stability
Chemical stability
0.25 bar (3.6 psi)
3 bar (43.5 psi)
short term: 4 -9 / long term: 7.5
Stable to all solutions commonly used in gel filtration including 8 M
urea and 6 M guanidine hydrochloride
Not stable in organic solvents!
-y-Aminophenyl-Adenosine triphospate (AP-ATP) immobilized on Agarose, suitable for purification of ATPbinding proteins.
Bulk material, syringe column and screening column based on Agarose. MPLC column based on Toyopearl
AF-650M.
®
®
Toyopearl is a trademark of TOSOH Bioscience.
Degree of substitution
20 µmol AP-ATP/ml gel
Storage buffer
20% Ethanol
1 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E‐Mail: info@mobitec.com www.mobitec.com Revised November 2011
For in vitro use only
Product Information Sheet
Order: # AC-101-JB
Immobilized gamma-Aminophenyl-ATP (C10-spacer), Syringe column - Agarose
References
Dhillon et al. (2009) The C-terminus of Raf-1 acts as a 14-3-3-dependent activation switch. Cellular
Signalling 21:1645.
Drewes et al. (1995) Microtubule-associated Protein/Microtubule Affinity-regulating Kinase (p110mark). J.
Biol. Chem. 270 (13):7679.
Haystead et al. (1993) -y-phosphate linked ATP-Sepharose for the affinity purification of protein-kinases rapid purification to homogeneity of sceletal-muscle mitogen-activated protein-kinase. Eur. J. Biochem.
214 (2):459.
Jeansonne et al. (2006) A rapid ATP affinity-based purification for the human non-receptor tyrosine kinase cSrc. Protein Expression and Purification 46:240.
Wu et al. (2010) 6-Alkylsalicylic acid analogues inhibit in vitro ATPase activity of heat shock protein 90. Arch
Pharm Res. 33(12):1997.
Yu et al. (2010) Withaferin A targets heat shock protein 90 in pancreatic cancer cells. Biochemical
Pharmacology 79:542.
Zhang et al. (2008) A novel Hsp90 inhibitor to disrupt Hsp90/Cdc37 complex against pancreatic cancer cells.
Molecular Cancer Therapeutics 7 (1):162.
Zitzler et al. (2008) Distinct binding sites for the ATPase and substrate-binding domain of human Hsp70 on
the cell surface of antigen presenting cells. Molecular Immunology 45:3974.
Order Information, Shipping and Storage
Order#
Product
AC-101C-JB
Immobilized gamma-Aminophenyl-ATP (C10-spacer), Syringe column - Agarose
AC-101L-JB
Immobilized gamma-Aminophenyl-ATP (C10-spacer), Bulk material - Agarose
AC-101MP-JB Immobilized gamma-Aminophenyl-ATP (C10-spacer), MPLC column - Toyopearl®
AC-101S-JB
Immobilized gamma-Aminophenyl-ATP (C10-spacer), Bulk material - Agarose
AC-101SC-JB
Immobilized gamma-Aminophenyl-ATP (C10-spacer), Screening column - Agarose
shipped on blue ice; store at 4 °C
Quantity
1 ml
5 ml
1 ml
1 ml
0,2 ml
Storage
Short term exposure (up to 1 week cumulative) to ambient temperature possible. Long term storage at
4 °C.
Shelf life: 12 month
Contact and Support
MoBiTec GmbH
Lotzestrasse 22a
D-37083 Goettingen
Germany
Customer Service – General inquiries & orders
phone: +49 (0)551 707 22 0
fax:
+49 (0)551 707 22 22
e-mail: order@mobitec.com
Technical Service – Product information
phone: +49 (0)551 707 22 70
fax:
+49 (0)551 707 22 77
e-mail: info@mobitec.com
MoBiTec in your area: Find your local distributor at
www.mobitec.com
2 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E‐Mail: info@mobitec.com www.mobitec.com Revised November 2011
For in vitro use only