NPGA Annual Meeting January 22, 2013 Kevin McPhee Associate Professor, NDSU Department of Plant Sciences Plant breeding basics Principles Objectives Methods Results for achieving objectives from 2012 yield trials • 1251E, 1251T, 1252 What we see – Phenotype 1. Results from genetic potential – genes 2. Modified by the environment 3. Interaction of genes with environment Genetic variation • High yielding • Disease resistance • Superior quality traits Recombination • Methods to combine desired traits from contrasting parents (high yield with high quality) Selection • Methods that will discriminate among desired and undesired types Increased seed yield Food quality traits Disease resistance • Ascochtya blight • Appearance • Powdery mildew • Cooking • Sclerotinia white mold • Protein • Fusarium wilt • Mineral nutrients • Fusarium root rot Agronomic performance • Shatter resistance • Lodging resistance • Viruses • Anthracnose • Stemphylium blight • Bacterial blight Existing Public varieties breeding programs (USDA-ARS) Germplasm accessions (USDA, ICARDA, ICRISAT) International breeding programs/exchanges Parent A High Yield Resistant to Stemphylium Blight Plump Seed Susceptible to Anthracnose Light Red Cotyledon Color Short Plant Height X F1 Parent B Low Yield Susc. to Stemphylium Blight Flat Seed Resistant to Anthracnose Dark Red Cotyledon Color Tall Plant Height Hand pollinations Crosses in GH and field Grow F1 to produce the greatest number of F2 Self- pollinating Make cross before the flowers open Flowers are small Agronomic Disease Quality Yield evaluations nurseries/screening assessments evaluations Alive or Dead Presence or absence Average Increasing Values Range Art of resistance/yield values is knowing where to make selection Minimizing environmental influence Ascochyta blight – chickpea (2-3 major genes) QTL Ascochyta blight – lentil (AbR1) QTL Anthracnose (LCt1, LCt2) QTL Powdery mildew (er1, er2, Er3) simple inheritance Root rots (many genes) QTL Fusarium wilt (Fw, Fnw) simple inheritance Virus (En, sbm-1, lr) simple inheritance North Central REC Williston REC Hettinger REC Langdon REC Carrington REC NPGA NDSU Shana Pederson Deven Styczynski Danielle Fiebelkorn Dana Beckedahl Kayla Graber Allison Walter Axel Morales Angela Christopher Many others not named individually