1. Introduction: Since the mid 19th century, societies have dramatically increased the level of public health by the appropriate separation and treatment of human wastes and unbalanced energy management due to increasing energy consumption. The world today is undoubtedly facing a serious energy crisis, and energy demand in the whole world continues to increase at an unsustainable pace. Possible options are carbonbased alternatives to oil, such as methane hydrates and conversion of coal into methane gas, and the use of less readily available oil reservoirs and oil shale even if the usage of these is critical. Climate change is being driven by the atmospheric release of greenhouse gases like CO2 (1), and an impending global energy crisis is coming at a time when we can least afford to release additional stored carbon. Nuclear power offers a carbon-free approach to energy generation, but no good solutions for nuclear waste exist. We must develop energy-saving technologies that can stretch oil reserves while we modify our energy-use patterns and infrastructure to become more sustainable over the next few decades and perhaps even centuries. The India, as a matter of fact, has very few means for generating electricity. Alternative sources of electricity such as hydroelectric power plants are still few in the country. In other states, including developing countries, methane digester systems are being used to harvest energy from biomass. However, using these systems is very expensive and is subject to Several processes including combustion that releases harmful gases (Bilcan et al, 2003). To provide a solution for the demand for energy, scientists have been studying diverse organisms that may take part in the search for an alternative source for electrical energy. In addition to these technologies an attractive and novel alternative for the conservation of chemical energy into electrical energy is the development of microbial fuel cells (MFCs) (Katz et al. 2003; lovely 2006). A microbial fuel cell is a device that converts chemical energy to electrical energy by the catalytic reaction of microorganisms (Allen and Bennetto 1993; Wingard et al. 1982., Reimers et al. 2001., Kim H.j. et al. 2002.). In addition, the MFCs offer an environmentally friendly alternative to fossil fuels (Lovely 2006; Katz et al. 2003). Initially, the concept of the electricity generation is given by M.C. Potter (Potter 1910, 1911). He stated that “the disintegration of organic compounds by microorganisms is accompanied by the liberation of electrical energy (Potter 1911). These fuel cells based on metabolic activity of microorganisms on the organic substrates which contains sugars as the main component. Fuel cells, if used for wastewater treatment, can provide clean energy for people, apart from effective treatment of wastewater. The benefits of using fuel cells include clean, safe, quiet performance; high energy efficiency; and easy to operating. Various research groups are keenly interested to improve the current density by more facile and efficient methods (Palmore and Whitesides 1994; Bond et al. 2002; Angenent et al. 2004; Pham et al. 2004). Generation of electrical energy is based on the principles of fermentation in which organic substrate undergo the biochemical reaction in the presence of microorganisms which result in the formation of the hydrogen fuel. The fuel so formed is finally into electrical energy and water through redox reaction. (Abdul majeed Khan.2009). Presently, research on MFCs Using wastewater as substrate is in the initial stages of laboratory evaluation around the world. The reported work so far is mainly based on using the monoculture at laboratory level (Rabaey et al. 2003, Park et al. 2003, Larminie et al. 2003.) The use of energy increasing day by day. Thus there is a need to search for alternatives to fossil fuel, utilizing renewable energy from waste organic source is the present trend of active research and in view of this bioelectricity generation through microbial fuel cell using variety of substrate is being studied extensively. It is well known that microorganisms can produce fuels from organic matters. In present study we have tried to generate electricity from wastewater using lactobacillus. The basic aim of the present study is to design microbial fuel cells employing low-cost materials without using toxic mediators, which will have the possibility to be implemented in the wastewater treatment plants in the economical perspective. In present study we have tried to generate electricity from wastewater using lactobacillus. Objectives Generally, this study aimed to: Waste water can be a possible alternative source of electricity. construct a MFC using inexpensive and local materials Specifically, this study sought to: Assess electricity generation from Sewage water in an MFC, employing lowcost materials without using toxic mediators, which will have the possibility to be implemented in the wastewater treatment plants in the economical perspective. Hypotheses Sewage water can be an efficient and cost-effective source of electricity. Advantages of MFC over current energy generating technologies from organics (Rabaey and Verstraete, 2005) • • • • • • • • • • • High conversion efficiency Ambient temperature No gas treatment No energy input for aeration Application for wide locations and diverse fuels Generation of energy out of wastewater. So far, max. 30 mA, 3600 mW/m2, 216 W/m3, 0.5 volts Direct conversion of substrate energy to electricity. Simple process chain. Less sludge production. Omission of gas treatment due to undesirable anaerobic off-gases not produced. Minimal Aeration necessary. Scope and Limitations This research project is specific to the feasibility of microorganisms as biocatalysts in a MFC.Also, the study also aimed to construct a MFC.using inexpensive and readily-available materials. The electricity produced will be on a small scale hence, further studies have to be done to explore the possibility of using Sewage water to produce electricity for home or industrial uses. Since the MFC technology is not yet well-understood, we are aware of our many limitations in a lot of things, specifically in methodology which we hope further studies could improve on. In addition, this study aims to open doors to interested scientists and companies to enhance the possibility of using microorganisms as alternative power sources to the ones being used today.