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Goat anti-Mouse IgG (H+L) Secondary Antibody,
RPE
Product data sheet
Catalog Number P-852
Details
Species Reactivity
1mL
Size
Tested species reactivity
Mouse
Published species reactivity
Not Applicable
Host/Isotope
Goat / IgG
Class
Polyclonal
Tested Applications
Dilution *
Type
Secondary Antibody
Flow Cytometry (Flow)
1-10 µg/mL
Immunogen
Gamma Immunoglobins Heavy and
Light chains
Published Applications
Target Class
IgG
Cross Adsorption
Against human IgG and human
serum prior to conjugation
Antibody Form
Whole Antibody
Conjugate
RPE
Form
liquid
Concentration
1 mg/ml
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage Conditions
4° C
Miscellaneous PubMed (MISC)
*
See 30 publications below
Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment
using appropriate negative and positive controls.
Background/Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection
, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP,
fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species
and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific
reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). No claim of suitability for use in applications regulated by FDA is made. The warranty
provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the Buyer. Any model or sample furnished to Buyer
is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.
NO OTHER WARRANTIES, EXPRESS OR IMPLED, ARE GRANTED INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER'S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD
IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER'S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR
NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS. Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and
is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to human or animals.
Thermo Fisher Scientific
3747 N. Meridian Road
Rockford, IL 61105 USA
thermofisher.com/contactus
PubMed References For Goat anti-Mouse IgG (H+L) Secondary Antibody, RPE
30 Miscellaneous PubMed References
Species / Dilution
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Summary
The Journal of biological chemistry (Jul 2006; 281: 19395)
"A structural determinant of human cytomegalovirus US2 dictates the down-regulation of class I major
histocompatibility molecules."
Author(s):Oresic K,Noriega V,Andrews L,Tortorella D
PubMed Article URL:http://dx.doi.org/10.1074/jbc.M601026200
The Journal of biological chemistry (Sep 2005; 280: 31936)
"Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and
beta-arrestins."
Author(s):Jacob C,Yang PC,Darmoul D,Amadesi S,Saito T,Cottrell GS,Coelho AM,Singh P,Grady EF,Perdue M,Bunnett NW
PubMed Article URL:http://dx.doi.org/10.1074/jbc.M506338200
Cell transplantation (Dec 2003; 12: 743)
"Improved efficacy of stem cell labeling for magnetic resonance imaging studies by the use of cationic liposomes."
Author(s):van den Bos EJ,Wagner A,Mahrholdt H,Thompson RB,Morimoto Y,Sutton BS,Judd RM,Taylor DA
PubMed Article URL:http://dx.doi.org/null
The Journal of biological chemistry (Apr 2003; 278: 14313)
"The extracellular domain of receptor activity-modifying protein 1 is sufficient for calcitonin receptor-like receptor
function."
Author(s):Fitzsimmons TJ,Zhao X,Wank SA
PubMed Article URL:http://dx.doi.org/10.1074/jbc.M211946200
The Journal of biological chemistry (Feb 2003; 278: 3751)
"Elucidation of Smad requirement in transforming growth factor-beta type I receptor-induced responses."
Author(s):Itoh S,Thorikay M,Kowanetz M,Moustakas A,Itoh F,Heldin CH,ten Dijke P
PubMed Article URL:http://dx.doi.org/10.1074/jbc.M208258200
The Journal of cell biology (Sep 2002; 158: 1079)
"Beta-catenin-induced melanoma growth requires the downstream target Microphthalmia-associated transcription
factor."
Author(s):Widlund HR,Horstmann MA,Price ER,Cui J,Lessnick SL,Wu M,He X,Fisher DE
PubMed Article URL:http://dx.doi.org/10.1083/jcb.200202049
The Journal of cell biology (Apr 2002; 157: 493)
"The integrin alpha(v)beta8 mediates epithelial homeostasis through MT1-MMP-dependent activation of TGF-beta1."
Author(s):Mu D,Cambier S,Fjellbirkeland L,Baron JL,Munger JS,Kawakatsu H,Sheppard D,Broaddus VC,Nishimura SL
PubMed Article URL:http://dx.doi.org/10.1083/jcb.200109100
The Journal of biological chemistry (Nov 2001; 276: 42182)
"Agonist-promoted internalization of a ternary complex between calcitonin receptor-like receptor, receptor
activity-modifying protein 1 (RAMP1), and beta-arrestin."
Author(s):Hilairet S,Bélanger C,Bertrand J,Laperrière A,Foord SM,Bouvier M
PubMed Article URL:http://dx.doi.org/10.1074/jbc.M107323200
Journal of immunology (Baltimore, Md. : 1950) (Sep 2001; 167: 2651)
"Intestinal macrophages lack CD14 and CD89 and consequently are down-regulated for LPS- and IgA-mediated
activities."
Author(s):Smith PD,Smythies LE,Mosteller-Barnum M,Sibley DA,Russell MW,Merger M,Sellers MT,Orenstein JM,Shimada T,
Graham MF,Kubagawa H
PubMed Article URL:http://dx.doi.org/null
Cell (Jul 2001; 106: 157)
"Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes."
Author(s):Reddy A,Caler EV,Andrews NW
PubMed Article URL:http://dx.doi.org/null
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). No claim of suitability for use in applications regulated by FDA is made. The warranty
provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the Buyer. Any model or sample furnished to Buyer
is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.
NO OTHER WARRANTIES, EXPRESS OR IMPLED, ARE GRANTED INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER'S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD
IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER'S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR
NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS. Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and
is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to human or animals.
Thermo Fisher Scientific
3747 N. Meridian Road
Rockford, IL 61105 USA
thermofisher.com/contactus
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
The Journal of biological chemistry (Apr 2001; 276: 13217)
"The TXP motif in the second transmembrane helix of CCR5. A structural determinant of chemokine-induced
activation."
Author(s):Govaerts C,Blanpain C,Deupi X,Ballet S,Ballesteros JA,Wodak SJ,Vassart G,Pardo L,Parmentier M
PubMed Article URL:http://dx.doi.org/10.1074/jbc.M011670200
The Journal of biological chemistry (Dec 2000; 275: 37757)
"Characterization of CD1e, a third type of CD1 molecule expressed in dendritic cells."
Author(s):Angenieux C,Salamero J,Fricker D,Cazenave JP,Goud B,Hanau D,de La Salle H
PubMed Article URL:http://dx.doi.org/10.1074/jbc.M007082200
Biochemistry (Oct 2000; 39: 12916)
"CD39L2, a gene encoding a human nucleoside diphosphatase, predominantly expressed in the heart."
Author(s):Yeung G,Mulero JJ,McGowan DW,Bajwa SS,Ford JE
PubMed Article URL:http://dx.doi.org/null
Journal of immunology (Baltimore, Md. : 1950) (Aug 2000; 165: 1395)
"IL-4 enhances keratinocyte expression of CXCR3 agonistic chemokines."
Author(s):Albanesi C,Scarponi C,Sebastiani S,Cavani A,Federici M,De Pità O,Puddu P,Girolomoni G
PubMed Article URL:http://dx.doi.org/null
The Journal of biological chemistry (Mar 2000; 275: 7481)
"Epidermal growth factor-stimulated tyrosine phosphorylation of caveolin-1. Enhanced caveolin-1 tyrosine
phosphorylation following aberrant epidermal growth factor receptor status."
Author(s):Kim YN,Wiepz GJ,Guadarrama AG,Bertics PJ
PubMed Article URL:http://dx.doi.org/null
The Journal of biological chemistry (Mar 2000; 275: 7795)
"Multiple discontinuous ligand-mimetic antibody binding sites define a ligand binding pocket in integrin alpha(IIb)
beta(3)."
Author(s):Puzon-McLaughlin W,Kamata T,Takada Y
PubMed Article URL:http://dx.doi.org/null
The Journal of biological chemistry (Feb 2000; 275: 3288)
"Mapping of Eps15 domains involved in its targeting to clathrin-coated pits."
Author(s):Benmerah A,Poupon V,Cerf-Bensussan N,Dautry-Varsat A
PubMed Article URL:http://dx.doi.org/null
The Journal of biological chemistry (Dec 1999; 274: 35119)
"Conformational and biochemical differences in the TCR.CD3 complex of CD8(+) versus CD4(+) mature lymphocytes
revealed in the absence of CD3gamma."
Author(s):Zapata DA,Pacheco-Castro A,Torres PS,Ramiro AR,San José E,Alarcón B,Alibaud L,Rubin B,Toribio ML,Regueiro
JR
PubMed Article URL:http://dx.doi.org/null
The Journal of biological chemistry (Jun 1999; 274: 18524)
"Trafficking of proteinase-activated receptor-2 and beta-arrestin-1 tagged with green fluorescent protein.
beta-Arrestin-dependent endocytosis of a proteinase receptor."
Author(s):Déry O,Thoma MS,Wong H,Grady EF,Bunnett NW
PubMed Article URL:http://dx.doi.org/null
The Journal of biological chemistry (Jun 1999; 274: 16257)
"Substance P-induced trafficking of beta-arrestins. The role of beta-arrestins in endocytosis of the neurokinin-1
receptor."
Author(s):McConalogue K,Déry O,Lovett M,Wong H,Walsh JH,Grady EF,Bunnett NW
PubMed Article URL:http://dx.doi.org/null
The Journal of cell biology (Feb 1999; 144: 777)
"Matrix valency regulates integrin-mediated lymphoid adhesion via Syk kinase."
Author(s):Stupack DG,Li E,Silletti SA,Kehler JA,Geahlen RL,Hahn K,Nemerow GR,Cheresh DA
PubMed Article URL:http://dx.doi.org/null
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). No claim of suitability for use in applications regulated by FDA is made. The warranty
provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the Buyer. Any model or sample furnished to Buyer
is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.
NO OTHER WARRANTIES, EXPRESS OR IMPLED, ARE GRANTED INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER'S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD
IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER'S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR
NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS. Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and
is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to human or animals.
Thermo Fisher Scientific
3747 N. Meridian Road
Rockford, IL 61105 USA
thermofisher.com/contactus
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
Not Applicable / Not Cited
The Journal of biological chemistry (Dec 1998; 273: 35332)
"Neisseria gonorrhoeae porin modulates phagosome maturation."
Author(s):Mosleh IM,Huber LA,Steinlein P,Pasquali C,Günther D,Meyer TF
PubMed Article URL:http://dx.doi.org/null
The Journal of biological chemistry (Oct 1998; 273: 28040)
"Regulation of chemotactic and proadhesive responses to chemoattractant receptors by RGS (regulator of G-protein
signaling) family members."
Author(s):Bowman EP,Campbell JJ,Druey KM,Scheschonka A,Kehrl JH,Butcher EC
PubMed Article URL:http://dx.doi.org/null
Proceedings of the National Academy of Sciences of the United States of America (Jul 1992; 89: 6020)
"Detection of rare antigen-presenting cells by the lacZ T-cell activation assay suggests an expression cloning
strategy for T-cell antigens."
Author(s):Karttunen J,Sanderson S,Shastri N
PubMed Article URL:http://dx.doi.org/null
Journal of immunological methods (May 1992; 149: 159)
"Flow cytometry analysis of the expression of neutrophil FMLP receptors."
Author(s):Allen CA,Broom MF,Chadwick VS
PubMed Article URL:http://dx.doi.org/null
The Journal of cell biology (Mar 1992; 116: 1291)
"Incorporation of protein into spore coats is not cell autonomous in Dictyostelium."
Author(s):West CM,Erdos GW
PubMed Article URL:http://dx.doi.org/null
Journal of immunological methods (Dec 1991; 145: 83)
"Human serum induced opsonization of immunoglobulin G-coated polystyrene microspheres with complement
components C3 and C4 as measured by flow cytometry."
Author(s):Spycher MO,Spycher-Burger M,Späth PJ,Burckhardt JJ
PubMed Article URL:http://dx.doi.org/null
Journal of immunological methods (Mar 1990; 128: 109)
"A biologic assay for IL-4. Rapid fluorescence assay for IL-4 detection in supernatants and serum."
Author(s):Custer MC,Lotze MT
PubMed Article URL:http://dx.doi.org/null
Journal of immunological methods (Dec 1989; 125: 265)
"Flow cytometric methods for the analysis of human basophil surface antigens and viability."
Author(s):Bochner BS,McKelvey AA,Schleimer RP,Hildreth JE,MacGlashan DW
PubMed Article URL:http://dx.doi.org/null
Journal of immunological methods (Jun 1989; 120: 291)
"Simple modification of a commercial flow cytometer to triple laser excitation. Simultaneous five-color fluorescence
detection."
Author(s):Woronicz JD,Rice GC
PubMed Article URL:http://dx.doi.org/null
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). No claim of suitability for use in applications regulated by FDA is made. The warranty
provided herein is valid only when used by properly trained individuals. Unless otherwise stated in the Documentation, this warranty is limited to one year from date of shipment when the Product is subjected to normal, proper and intended usage. This warranty does not extend to anyone other than the Buyer. Any model or sample furnished to Buyer
is merely illustrative of the general type and quality of goods and does not represent that any Product will conform to such model or sample.
NO OTHER WARRANTIES, EXPRESS OR IMPLED, ARE GRANTED INCLUDING WITHOUT LIMITATION, IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR ANY PARTICULAR PURPOSE, OR NON INFRINGEMENT. BUYER'S EXCLUSIVE REMEDY FOR NON-CONFORMING PRODUCTS DURING THE WARRANTY PERIOD
IS LIMITED TO REPAIR, REPLACEMENT OF OR REFUND FOR THE NON-CONFORMING PRODUCT(S) AT SELLER'S SOLE OPTION. THERE IS NO OBLIGATION TO REPAIR, REPLACE OR REFUND FOR PRODUCTS AS THE RESULT OF (I) ACCIDENT, DISASTER OR EVENT OF FORCE MAJEURE, (II) MISUSE, FAULT OR
NEGLIGENCE OF OR BY BUYER, (III) USE OF THE PRODUCTS IN A MANNER FOR WHICH THEY WERE NOT DESIGNED, OR (IV) IMPROPER STORAGE AND HANDLING OF THE PRODUCTS. Unless otherwise expressly stated on the Product or in the documentation accompanying the Product, the Product is intended for research only and
is not to be used for any other purpose, including without limitation, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption by or application to human or animals.
Thermo Fisher Scientific
3747 N. Meridian Road
Rockford, IL 61105 USA
thermofisher.com/contactus