LAGUARDIA COMMUNITY COLLEGE
CITY UNIVERSITY OF NEW YORK
NATURAL SCIENCES
SCB 260 - GENERAL MICROBIOLOGY
Course Coordinator: Olga Calderon, PhD
Contact Info: ocalderon@lagcc.cuny.edu or 718-482-5749 Office M-220A
CATALOGUE DESCRIPTION
An introduction to microorganisms found in nature, industry and disease. Students are introduced to virology,
bacteriology, immunology, epidemiology, pathology and other related areas of microbial physiology. The
laboratory will deal with the isolation and identification of common pathogenic and nonpathogenic organisms
utilizing techniques of staining, culturing, fermentation reactions and microscopic inspection.
PREREQUISITES
SCB202 or SCB204 or SCB209
REQUIRED TEXTS
LABORATORY MANUAL – PAPER COPY – NO ELECTRONIC COPY PERMITTED
Title:
Microbiology Laboratory Theory & Application, 2nd Edition
Authors:
Michael J. Leboffe, Burton E. Pierce
Publisher:
Morton Publishing Company
Copyright:
2008
ISBN-10:
0895829479
ISBN-13:
978-0895829474
TEXTBOOK
Title:
Microbiology: An Introduction, Books a la Carte Plus MasteringMicrobiology, 12/E
Authors:
Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher:
Benjamin Cummings
Copyright:
2010,2013,2016
ISBN-10:
0321793110
ISBN-13:
9780321793119
ISBN-loose-leaf: 9780133983722
ATLAS (highly recommended, but optional)
Title:
Microbiology: A Photographic Atlas for the Laboratory: 1st Edition
Authors:
Steven K. Alexander Ph.D., Dennis Strete.
Publisher:
Benjamin Cummings. Copyright: 2000
ISBN-13:
9780805327328
Grading
Criteria
Lecture
Laboratory
4 Exams
4 X 10 each =
40%
*4 post-lab quizzes
4X3=
12%
3 HWs
3 x 3.33ea =
10%
4 pre lab quizzes
3 X 1=
3%
1 Paper
1 x 10 =
10%
1 Morph. Unknown
1X5=
Individual Unknown
*The lowest grade for one quiz for lab will be dropped
5%
20%
COURSE GRADE
A
=
93 – 100
C
=
73 – 76.9
A-
=
90 – 92.9
C-
=
70 – 72.9
B+
=
87 – 89.9
D+
=
67 – 69.9
B
=
83 – 86.9
D
=
63 – 66.9
B-
=
80 – 82.9
D-
=
60 – 62.9
C+
=
77 – 79.9
F
=
<59.9
1
ATTENDANCE
Attendance is mandatory based on the College’s policy. Both lecture and laboratory! Attendance will be taken
at each session.
INSTRUCTIONAL OBJECTIVES
1.
2.
3.
4.
To introduce the student to the field of microbiology; showing its medical and economic importance.
To develop student skills in the area of diagnostic bacteriology and parasitology.
To show the importance of sanitation in controlling infection.
To develop a student awareness of epidemiology.
PERFORMANCE OBJECTIVES
1.
To acquire the techniques and manual dexterity necessary for the safe handling of potentially harmful
organisms for his own and others protection.
2. To consider no bacteria completely harmless.
3. To use the methods and precautions essential in the collecting and handling of pathogenic and non-pathogenic
specimens for microbiological examination.
4. To use practical, efficient methods of disinfection and sterilization wherever necessary.
5. To use identification methods necessary to recognize pathogens.
6. To recognize the importance of good public health departments and good sanitation in maintaining a healthful
environment.
7. To realize the dangers of the spread of disease due to high-speed transportation and the value of the work of
the World Health Organization and others.
8. To understand that the problem of controlling infectious disease is greater than that of attacking the harmful
microorganisms involving the body's resistance or susceptibility, the body's defense and the problem of
immunization.
9. To apply the precautions learned in the laboratory to daily living e.g. hands kept away from eyes and mouth,
care of food to prevent contamination, prevention of spread of infection in the home, hospital, and the
community.
10. To appreciate the value of microorganisms as experimental tools and biological models.
EXAMINATION MAKE-UP POLICIES
LECTURE EXAMS
There are four (4) major exams. If you are absent for any exam, a make-up will be permitted on your own
time. Make-ups for exams will be administered only after documented absence (doctor’s note, etc.). The makeup exams tend to be considerably more difficult than the scheduled exam.
LABORATORY QUIZZES
If you are absent or late, there will be no make-ups permitted. However, the lowest quiz grade will be
dropped when calculating your quiz average for laboratory.
LABORATORY SAFETY (Dress code/ Personal Protective Equipment)
•
•
•
•
•
•
Students participating in microbiology labs must have their own lab coat for every lab. No exceptions.
No open-toed shoes are allowed in the lab.
Gloves are required for standard lab procedures if proper hand hygiene is performed; however students
should wear gloves if their hands have fresh cuts or abrasions. Proper hand hygiene involves thorough hand
washing prior to and immediately after finishing handling microorganisms.
Safety glasses/ goggles are not required for standard lab procedures but they are permitted. It is
recommended that students do not wear contact lenses to lab to prevent the risk of contamination; instead
corrective lenses should be worn underneath a pair of splash-resistant safety goggles.
Students not following the laboratory dress code and safety guidelines will not be permitted to attend
the lab.
Use of cell phones in class is prohibited. Cell phones must be turned off or on silent mode during class.
2
POLICY ON CHEATING
Instructors of this course are required to implement College policy regarding cheating on examinations and
quizzes. A complete statement is available through student counseling services.
A synopsis is as follows:
If an instructor suspects a student of cheating, or any of the violations listed below, the instructor will inform
the student of his or her suspicion, and a student/teacher conference will be held. At that conference the
suspected violation and the instructor's intended penalty for the violation will be discussed.
1. Give the student a copy of the STUDENT HANDOUT ON LAGUARDIA ACADEMIC
INTEGRITY POLICY (this document) and answer any questions the student may have.
2. Inform the student of the reasons for his or her suspicions and the intended penalties.
These penalties may include, but are not limited to, the following:
An "F" on the paper, quiz, assignment or examination involved and an "F" for the course.
3. If the student admits guilt, and agrees on the penalty, he/she should indicate so on the formal complaint.
The instructor will then send the complaint to the Office of Academic Standing and impose the penalty.
4. If the student does not admit guilt or agrees to the penalty, the student/teacher conference will end and a
hearing on the issue must be held. The instructor will then submit the complaint to the Office of Academic
Standing, which will send a copy to the Dean of Students who will then begin disciplinary proceedings.
If the records of the Office of Academic Standing indicate that the student has committed a similar offense
before the Office of Academic Standing will:
Send a copy of the complaint to the Dean of Students, who will begin disciplinary proceedings
INCOMPLETE POLICY
The following is the policy on incomplete grades. There will be no exceptions to the rule.
An incomplete grade is assigned ONLY if ALL of the following conditions have been met:
•
The student is missing only one or two assignments.
•
The student has complied with the attendance policy.
•
The student presents the instructor with documentation explaining the reason he/she is unable to
complete the assignment before the end of the semester.
A student will not be given an incomplete if the student disappears at the end of the semester. Both student
and instructor must complete an Incomplete Contract.
ADDITIONAL INFORMATION
Office hours
Will be announced by your instructor
Quiz dates/Assignment due dates
The days and times of the quizzes and exams will be announced well in advance.
College Calendar
In order to allow for school holidays, it is often necessary to hold classes on days of the week
other than those originally scheduled. Consult the College Catalog for changes.
Official Withdrawal Date
Consult the College Catalog for Official Withdrawal Date.
Student Rights and Responsibilities
Consult the Student Handbook and College Catalog.
3
LECTURE OUTLINE
TOPIC
History of microbiology
TEXTBOOK
Ch. 1; Ch. 2 (p. 33); Ch. 3 (p. 58)
Survey of Microbial Life
Appendix D & F
Functional Anatomy of Prokaryotic & Eukaryotic cells
Ch. 4 (pp. 76 - 98)
(Review pp. 98-end)
Ch. 5; (Review selected sections of
Ch. 2) - Appendix A – glycolysis and
the Krebs cycle
Ch. 6 (pp. 157 – 167); (174 – 177)
Microbial Metabolism
Microbial Growth
Exam #1
Classification of Microorganisms
Ch. 10
The Prokaryotes: Domains Bacteria and Archaea
Ch. 11
The Eukaryotes: Fungi, Algae, Protozoa and Helminths
Ch. 12 (selected readings: Appendix
F)
Ch. 13; (Appendix F)
Viruses, Viroids, and Prions
Exam #2
The Control of Microbial Growth
Ch. 7
Antimicrobial Drugs
Ch. 20
Principles of Disease & Epidemiology
Ch. 14
Microbial Mechanisms of Pathogenicity
Ch. 15
Innate Immunity: Nonspecific Defenses of the Host
Ch. 16
Exam #3
Adaptive Immunity: Specific Defenses of the Host
Ch. 17
Practical Applications of Immunology
Ch. 18
Biotechnology, Recombinant DNA
Ch. 9
Disorders Associated with the Immune System
Ch. 19
Exam #4 (during final exam week)
4
LABORATORY OUTLINE
LABORATORY
1
2
3
4
5
6
7
•
•
•
•
•
TOPIC
Lab Safety (PowerPoint presentation & Handout)
Aseptic Technique
Isolation of bacteria by dilution techniques
Start microscopy
Microscopy
Recording culturing results from last lab
Staining Techniques:
•
Preparation of smears and simple staining
•
Negative staining
•
Gram staining
Staining Techniques (cont.)
•
Acid Fast Staining
•
Structural Staining
10-12
Exercises 3-4, 3-5, 3-6
Exercises 2-2, 2-3, 2-4
Staining Techniques (cont.)
•
Morphological Unknown
•
Morphological unknown report must be submitted at
the end of this session
•
Microbes in the environment
•
Special media for isolating bacteria
•
Oxygen and the growth of bacteria
•
Temperature and pH response
Exercises 3-7, 3-8, 3-9, 310, 3-11 (theory only)
Microbial metabolism/Biochemical tests (Part I)
Exercises 5-1, 5-2, 5-3,
5-4, 5-5, 5-6, 5-7, 5-8
Recording results from Special media, Oxygen req., Temp
and pH
Microbial metabolism/Biochemical tests (cont.) (Part II)
Exercises 2-6, 2-7, 2-8, 43, 4-4, 4-5, 4-6
Exercises 5-9, 5-10, 5-11,
5-12, 5-13, 5-14, 5-15, 516, 5-18; 9-4 & 9-5 (theory
only)
Recording results from Microbial metabolism/Biochemical
tests (Part I)
Exercises 5-1, 5-2, 5-3,
5-4, 5-5, 5-6, 5-7, 5-8
Physical & Chemical Methods of Control:
•
Ultraviolet Radiation Damage & repair
•
Disinfectants and Antiseptics
•
Antimicrobial Drugs
Exercises
2-12 & 2-13 (theory only),
5-20
7-2, 8-2
Recording results from Microbial metabolism/Biochemical
tests (Part II)
Exercises 5-9, 5-10, 5-11,
5-12, 5-13, 5-14, 5-15, 516, 5-18
Exercises
9-1, 9-2, 9-3 (as
applicable) Table A-6
(p529), Bergey’s manual,
online resources, etc
8
9
LAB BOOK
Lab Safety pages (pg. 1-6)
Exercises 1-3 (theory), 14, 1-5, 1-6 (theory).
3-1
Exercises 3-1,3-2, 3-3,
Individual Unknown Project
Students must submit their unknown report together with a
Gram reaction slide no later than week 12.
Exercise 1-1
Exercises 2-6, 2-7, 2-8, 29, 4-3, 4-4, 4-5, 4-6
5