PSU College of Medicine Core Facilities (May, 2015)
Section of Research Resources H093
(Additional information available at med.psu.edu/core)
Summary of Facilities and Services*
*Detailed descriptions in 2nd half of this document
•Macromolecular Core Facility (updated 5/4/15)
The purpose of the Macromolecular Core Facility is to provide state-of-the-art methods for analysis and
synthesis of protein and nucleic acid structures. Current services include: Protein/peptide sequencing
(Mass Spec), Peptide synthesis, Oligonucleotide synthesis, and Film Image analysis.
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/macromolecular-synthesis
Director: Dr. Bruce Stanley (bstanley@psu.edu)
Senior Research Technologist & Lab Manager: Anne Stanley (aes7@psu.edu)
Research Technologist: Suja Maddukuri (srm25@psu.edu)
•Microscopy Imaging Facilities (updated 2/6/15)
The MIF provides services in ultra-high resolution imaging of cells and tissues in fixed or live states. We
also provide expert services in quantitative image analysis and consultations on microscopy-related
research projects. The MIF houses: (1) a high end inverted confocal microscope system [Leica SP8
AOBS White Light Laser] and a sophisticated inverted wide field microscope with optical sectioning
and deconvolution capabilities [DeltaVision Elite] which are capable of high resolution 3D or 4D
fluorescence imaging of histological tissue sections (5-100 microns thick sections) or live/ fixed cells; (2)
a transmission electron microscope [JEOL 1400 TEM] capable of ultra-structural biological imaging; (3)
a cryo-transmission electron microscope [JEOL2100 Cryo-TEM] capable of single particle and single
macromolecular complex imaging; (4) image processing workstations [Bitplane Imaris and Huygens]
for complex 3D or 4D fluorescence image processing and quantitative image analysis; (5) image
processing workstations [auto3dem and EMAN2] for cryo-TEM image processing and 3D
reconstruction.
For the most up-to-date information including recent publications, see the facility’s web page and links at
http://med.psu.edu/web/core/microscopy-histology.
Director: Dr. Thomas Abraham (tabraham@hmc.psu.edu)
Lab Managers: Confocal Microscopy – Wade Edris (wedris@hmc.psu.edu)
Electron Microscopy – Roland Myers (rlm9@psu.edu)
CryoEM – Bob Ashley (bobashley@psu.edu )
Histology – Dr. Kang Li (kxl30@psu.edu)
•Flow Cytometry Core Facility (updated 7/15/14)
Both Clinical samples and Research samples are routinely analyzed in this clinically (CAP)–accredited
facility. Two 4-color Becton Dickinson FACSCaliburs, one 8-color Becton Dickinson FACSCantoII, one
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6-color Becton Dickinson FACSCanto, a 15-color Becton Dickinson LSR II, a 17-color BectonDickinson Fortessa, and a 16-color Becton Dickinson Aria III high-speed 4-way sorter are available for
use by investigators at the College of Medicine.
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/flow-cytometry
Faculty Director: Dr. Todd Schell (tschell@hmc.psu.edu)
Research Project Manager & Flow Cytometry Specialist: Nate Sheaffer (nas2@psu.edu)
Research Technologists: Joe Bednarczyk (jbednarczyk@psu.edu)
Jade Vogel (jvogel@hmc.psu.edu).
•Genome Sciences Core Facility (updated 1/16/15)
The Penn State Hershey Genome Sciences Facility is a full service facility and provides consultation,
instrumentation, and services to both Penn State and non-Penn State investigators in genomic,
epigenomic, and transcriptomic studies (http://www.pennstatehershey.org/web/core/gene-expressionanalysis-overview). The variety of instrumentation allows for capabilities ranging from highly focused
analysis of candidate SNPs, and mRNAs to whole genome, exome, epigenome, and transcriptome
sequencing. Services are also available for a variety of study designs extending from a few laboratory
samples to large (100s to 1,000s of samples) clinical projects. Full bioinformatics service is also available
for data analysis.
The Facility resides in 5,000 sq ft of newly renovated space, encompassing separate “pre-amplification”
and “post-amplification” rooms to prevent any contamination of PCR-amplified materials to preprocessed input DNA/RNA samples. Four well-experienced staffs are available for assisting project
operation. In addition, the lab space is available for investigators who need temporary room for sample
preparation.
We receive either tissue, DNA/RNA, or customer-generated NGS libraries. We process samples
according to the prior consultation and agreement on the design of experiment. We develop new
applications to accommodate state-of-the-art NGS technologies. We conduct sequencing reads alignment,
secondary analysis (quantitation, variant calling, functional annotation, visualization, etc) and follow-up
the interpretation of the results. We support grant writings and educate/train students/post-docs for handson NGS processing.
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/gene-expression-analysis.
Director: Dr. Yuka Imamura (yimamura@hmc.psu.edu)
Research Technologist & Lab Manager: Rob Brucklacher (rbrucklacher@psu.edu)
Research Technologists: Georgina Bixler (gbixler2@hmc.psu.edu)
Elizabeth Conroy (econroy@hmc.psu.edu)
•Mass Spec & Proteomics Facility (updated 5/4/15)
This facility provides multiple separation, digestion, chemical derivatization, mass spec, and database
searching services for proteomic, carbohydrate, oligonucleotide, lipidomics, and small molecule analysis.
Analyses available including targeted methods for quantitation of pre-determined metabolites or proteins,
data-dependent discovery methods to ID and quantitate hundreds to thousands of metabolites or proteins,
and SWATH/Data-Independent Analyses (DIA) for simultaneous identification and quantitation.
Instrumentation includes an ABSciex TripleTOF 5600, an ABSciex MALDI TOF-TOF 5800; an
MDS/Sciex 4000 QTrap (Hybrid Ion Trap); a Waters Synapt HDMS; and a Voyager DE-PRO MALDITOF; an ABI Tempo LC-MALDI Plate Spotter; Shimadzu, Eksigent, Agilent 1100, Waters Acquity and
NanoAcquity HPLC and UPLC systems; and a Beckman-Coulter PF-2D system for whole protein level
separations and quantitation.
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For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/proteins-mass-spectometry.
Director: Dr. Bruce Stanley (bas12@psu.edu)
Lab Manager (Proteomics): Anne Stanley (aes7@psu.edu)
Lab Manager (Small Molecules): Dr. Dongxiao Sun (dsun@hmc.psu.edu)
•MRI/MRS Facility (updated 10/07/14)
The MRI/MRS Core Facilities are part of the Center for NMR Research (CNMRR), and provides
Magnetic Resonance Imaging (MRI), functional MRI (fMRI) and Magnetic Resonance Spectroscopy
(MRS) services for in vivo / ex vivo spectroscopy and imaging in animals and humans. Instrumentation
includes (1) a 3.0T Siemens Tim Trio with 18-receiver / 8-transmit channels, latest hardware and
software packages with full clinical capability; (2) a 7.0 T Biospec 70/20as small animal imaging system.
For the most up-to-date information, see the facility’s web page and links at
http://www.pennstatehershey.org/web/mricore/home/overview
Faculty Director: Dr. Qing Yang (qyang@hmc.psu.edu)
Research Project Manager: Jeff Vesek (jvesek@hmc.psu.edu)
Research Technologist: Patti Miller (pmiller5@hmc.psu.edu)
•Solution Phase NMR Facility (updated 1/23/13)
Facility includes both 500 MHz and 600 MHz Bruker NMRs with multiple cryoprobes for
macromolecular structure determination, organic chemistry and metabolomics studies.
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/mrinmr/solution-nmr
Director: Dr. Fang Tian (ftian@psu.edu) 717-531-6775
Project Manager: Dr. Jyh Ming Lin (jml47@psu.edu)
717-531-3612 (Office), 717-531-5340 (NMR Lab)
•Transgenic Mouse Facility - Department of Comparative Medicine, (updated 1/23/13)
This facility, located in the Animal Research Farm (ARF 173,136,144) under the auspices of the
Department of Comparative Medicine, can provide injection and breeding services for development of
knockout or transgenic mouse lines, as well as cryo- and sperm-preservation and rederivation services. To
create such transgenic or knockout lines, the core lab performs microinjection of purified DNA into the
pronucleus of fertilized mouse eggs on two separate injection days. Eggs are then returned to
pseudopregnant females, and founder lines established. The core lab harvests eggs, injects them on two
separate injection days, re-implants eggs, and coordinates litter stocks up to the establishment of a
founder line. Procedures for targeted gene disruption using embryonic stem cells have also been
developed, and over 80 transgenic and knockout mouse lines have been created.
For the most up-to-date information, see the facility’s web page and links at
http://infonet.hmc.psu.edu/animal-resources-program/services/Transgenic core home.htm
Director: Dr. Ronald Wilson (rpw4@psu.edu)
Lab Manager: Alane Seidel (aks2@psu.edu)
•Bioluminescent/Fluorescent Imaging Facility (updated 11/5/14)
The facility’s Xenogen IVIS 50 instrument provides a non-invasive way to track and quantify
bioluminescent and fluorescent signals in cells or in whole animals. Because the measurements do not
involve sacrificing the animal, serial measurements or time courses can be obtained, and the response to
multiple different treatments can be measured while minimizing inter-animal variability.
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For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/whole-animal-luminescent-imaging-services
Director: TBA
•Tecan EVO Robotics/Liquid handling Facility (updated 7/15/14)
The facility provides automated sample preparation, dilution, mixing, and aliquoting, using a Tecan
Freedom EVO system.
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/tecan-liquid-handline-robotics
Director: Dr. Carla J. Gallagher (cgallagher@hmc.psu.edu).
FACILITY DETAILS
•Macromolecular Core Facility (C1732) (updated 5/4/15)
Peptide synthesis: A Tribute Peptide Synthesizer from Protein Technology and a Milligen 9050 Fmoc
peptide synthesizer are available for peptide synthesis. A standard synthesis delivers between 50250 mg of peptide. A standard synthesis on either of these machines delivers between 50-80 mg
of peptide, at a cost of $20 per residue (0.1 mmol scale), or $30 per residue (0.2 mmol scale).
Custom syntheses are also possible (e.g., phosphopeptides, biotinylated peptides), with the
additional cost being determined by the reagents used. Mass Spec validation of the synthesized
peptide is available for an additional $15.
Oligonucleotide synthesis: A PolyGen DNA synthesizer and an Expedite 8909 DNA synthesizer are
used to generate oligonucleotides. Depending on the scale of the reaction, costs range from $0.33
- $1 per base, with no setup fee. When requested, purification is performed using Glen-pak
DNA Purification cartridges at a cost of $15 per oligo. Custom syntheses such as modified
bases (e.g., methylated bases, non-standard nucleotides) and phosphorothioate chemistries are
also available, with the price depending on the cost of the specialized reagent necessary.
Image analysis: A BioRad GS 800 densitometer and Quantity One and PDQuest software are
available for acquisition and quantitation/analysis of film images. A Typhoon fluorometer is also
available for DIGE gel image acquistion, with spot analysis through DeCyder software). After
image acquisition and analysis, spot cut lists can then be exported to our BioMachines/LEAP
Technology 2DiD spot cutter, or to an Ettan Spot Picker, for subsequent proteomic analyses
(See Mass Spec & Proteomics Facility section below). These imaging instruments are available
on a signup basis, at a rate of $5/hr.
The purpose of the Macromolecular Core Facility is to provide state-of-the-art methods for analysis and
synthesis of protein and nucleic acid structures. The Macromolecular Core Facility was established in
1987-1988, with primary grant support from the National Science Foundation (Macromolecular
Instrumentation for Cell and Molecular Biology; NSF DR-8804758, 11/1/88 - 4/30/91; $258,222 direct
costs; Drs. S. Goodman, A. Hopper, M. Billingsley, Co-PIs). The facility has also received grant support
from the NIH (Two-Dimensional Image Analyzer, NIH-RR04811, 5/1/89 - 4/30/90; $72,000 direct costs,
Dr. M. Billingsley, PI; Instrumentation for Peptide Analysis, NIH RR14851, 4/1/2001-3/31/2002, Dr. B.
Stanley, PI), the State of Pennsylvania via the Ben Franklin Partnership (Macromolecular Synthesis
Facility; 9/89 - 8/91; $100,000 direct costs; M. Billingsley, PI), and matching funds from the College of
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Medicine (approximately $500,000 in salary support, fringe benefits, supplies, new equipment, and
equipment upgrades.
Director: Dr. Bruce Stanley (bstanley@psu.edu)
Senior Research Technologist & Lab Manager: Anne Stanley (aes7@psu.edu)
Research Technologist: Suja Maddukuri (srm25@psu.edu)
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/macromolecular-synthesis
•Microscopy Imaging Facilities (C1724-C1730) (updated 2/6/15)
These facilities, which were initially co-supported between the Section of Research Resources and the
Juvenile Diabetes Diabetic Retinopathy Center grant, provide Confocal, Deconvolution, CryoEM, and
Transmission Electron Microscopy instrumentation and services, with additional Histology services
provided in the MMPRL (Morphologic and Molecular Pathology Research Lab).
Current Services and Equipment:
Leica SP8 Inverted Confocal (Room C1730) is equipped with both tunable emission filter technology
(AOBS) and a tunable pulsed white light laser source for the entire excitation spectrum. Leica
SP8 is capable of generating submicron level spatially- and spectrally-resolvable multicolor 3D or
4D fluorescent images in live or fixed cells/ tissues. Other specific features of this instrument
include fully automated operational procedures, high numerical aperture immersion objectives,
transmission light detection for the differential interference contrast (DIC) imaging, line and area
scanning capabilities with simultaneous or sequential scanning options, ultra-high speed live cell
imaging, complex cell tracking experiments, FRAP measurements, ratio metric experiments,
complex FRET measurements, tile scanning (scanning whole slide), hybrid signal detection
systems which allow 3D or 4D image generation at much lower light energies, time-gated
fluorescence signal collection and single photon counting. This new confocal microscope is also
equipped with very sophisticated live cell stage heater and CO2 chamber for perfusion system
[Tokai Hit, Japan] that enables complex live cell experiments continuously for several days.
Unassisted Use: $35/hour; Assisted Use: $50/hour; Off Peak Hours (8pm-8am): $23/hour
DeltaVision Elite Inverted Microscope (Room C1728) is equipped with precise optical sectioning
capability and deconvolution software. Other specific features of this instrument include multiwavelength switching excitation light source, high numerical aperture immersion objectives,
highly sensitive cooled CCD and EM-CCD cameras particularly useful for low light dose
fluorescence experiments, and transmission light detection for differential interference contrast
(DIC) imaging. This microscope is uniquely designed for generating multicolor 3D or 4D
fluorescent images in ultra-high speed live cell imaging mode as well as ultra-fast tile scanning
mode, with low phototoxicity potential.
Unassisted Use: $23/hour; Assisted Use: $35/hour
Bitplane Imaris and Huygens (Room C1730) image processing work station facilitates 3D and 4D
reconstructions to visualize and analyze multiple color images from wide-field and confocal
microscopes. Other functions include surface renderings, deconvolution, segmentation,
fluorescence Intensity measurements, complex cell tracking measurements, voxel counting, drug
bio-distribution measurements, accurate colocalization measurements and many other complex
image processing and statistical calculations depending upon researchers’ needs.
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JEOL 1400 TEM (Room C1727) is capable of generating ultra-structural nanoscale images from fixed
cell/tissue samples or multiplexed immune-labeled samples. Other specific features of this
instrument include fully automated computer-controlled operations, resolution as low as 3
Angstroms, magnification up to 370,000X and accelerating voltages ranging from 20-120kV, and
a bottom-mount GATAN camera capable of collecting data suitable for 3D reconstructions of
negative stained samples. The core technical staff has over 30 years of experience in sample prep,
tissue processing and embedding, ultramicrotomy, negative staining and multiplexed
immunolabeling with sized gold conjugates.
Unassisted Use: $45/hour; Tech time: $43/hour
JEOL 2100 Cryo-TEM (Room C1724) is capable of generating nanoscale images of single particle (e.g.
virus particles), phage, structures as small as proteins to whole cells, and capable of capturing
dynamic complexes while samples maintained at fully hydrated native biological state. Other
specific features of this instrument include tilt stage and serial EM software which enables 3D
tomography.
Auto3dem or EMAN2 (Room C1725] work station enables 3D reconstruction and visualization of CryoEM images. PyMOL, Chimera, Veda, or Situs software program facilitates fitting, and
interpretations of Cryo-TEM data.
Future Directions: An NIH Shared Instrumentation Grant for a Multiphoton Microscopy System was
submitted March 2014 (Dr. Thomas Abraham, P.I.), and was awarded in April 2015. Installation in Room
C1728A is planned for May-June 2015.
Multichannel Fluorescence Microscopy (C5742): A Nikon TE-2000U inverted microscope with 4channel epifluorescence and DIC modules, CoolSnap ES CCD camera, and NIS-Elements imaging
software is available in room C5742 (Grigoryev lab).
Fees: currently there are no fees for the use of these instruments.
Please contact Dr. Sergei Grigoryev (sgrigoryev@psu.edu) to arrange use of the Nikon TE2000U microscope
Histology (now part of the Morphologic and Molecular Pathology Research Lab C7716): Services
provided include Cryostat sectioning, Tissue processing and paraffin embedding, Paraffin sectioning,
Routine hematoxylin and eosin staining, specialized staining (glycogen, neuron, connective tissue
staining, etc.), Immunohistochemistry, and In situ hybridization. In addition to these procedures
performed routinely in the lab, specialized staining procedures can be implemented in collaboration
with individual investigators. Fees depend on exact work done, but tissue slicing and slide mounts are
$1 per slide.
Service
Fee
Tissue Processing & Paraffin Embedding
$3.00 per block
Paraffin Sectioning
$1.00 per slide
Cryostat Sectioning
$1.00 per slide
Staining (H&E, Glycogen, Neuron, Connective
Tissue, etc.)
$1.00 per slide
Decalcification
$1.00 per specimen
Immunohistochemistry
Variable
Training
$20.00 per hour
Supplies
$0.81 per slide
The Leica SP2 confocal microscope and QED/deconvolution microscopes were established in March,
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2004 using funds from the NIH (Instrumentation for Confocal Microscopy, (PHS grant number 1 S10 RR
16861-01), 7/1/2002-6/30/2003, Dr. B. Stanley, PI), funds from a JDRF Program Project Grant in
Diabetic Retinopathy (Dr. T. Gardner, P.I.) as well as institutional funds. The Histology and Electron
Microscopy Facilities were previously established as local departmental facilities within the Department
of Neuroscience and Anatomy, and became part of the institutional Shared Facilities in July, 2003).
Additional Shared Instrumentation grants acquired our Leica SP8 confocal (July, 2012; Dr. Coin
Barnstable, PI) and JEOL 2100 CryoEM (April 2012,; Dr. Susan Hafenstein, PI), and Institutional
Tobacco Settlement funds through the Office of our Vice-Dean for Research, Dr. Daniel Notterman
provided matching funds for the CryoEM acquisition as well as for the construction/renovation of the
Facility in the Fall of 2012. These services allow researchers to perform state-of-the-art imaging
experiments, thus greatly enhancing the Cell Biology capacities at the institution. As with all other core
facilities, fees are recalculated each year to cover maintenance, consumables, and salary costs only.
Director: Dr. Thomas Abraham – (tabraham@hmc.psu.edu)
Faculty Director for CryoEM: Dr. Susan Hafenstein (shafenstein@hmc.psu.edu)
Lab Managers: Confocal Microscopy – Wade Edris (wedris@hmc.psu.edu)
Electron Microscopy – Roland Myers (rlm9@psu.edu)
CryoEM – Bob Ashley (bobashley@psu.edu)
Histology – Dr. Kang Li (kxl30@psu.edu).
For the most up-to-date information including recent publications, see the facility’s web page and links at
http://med.psu.edu/web/core/microscopy-histology.
•Flow Cytometry Core Facility (C3603) (Cell Identification/Cell Kinetics Lab) (updated 7/15/14)
Both Clinical samples and Research samples are routinely analyzed in this clinically (CAP)–accredited
facility. Two 4-color Becton Dickinson FACSCaliburs, one 8-color Becton Dickinson FACSCantoII, one
6-color Becton Dickinson FACSCanto, a 15-color Becton Dickinson LSR II, a 17-color BectonDickinson Fortessa, and a 16-color Becton Dickinson Aria III high-speed 4-way sorter are available for
use by investigators at the College of Medicine.
Fees: Rates for Flow Cytometer Use
Standard Sample Acquisition (Researcher provides operator)
Standard Data Analysis on Computer (Researcher provides operator)
Assisted Sample Acquisition (Core provides operator)
Additional Set up Charge for Sorting on the Aria III
Aria III Sorting will be charged at a rate of:
$50.00 per hour
$20.00 per hour
Additional $50.00 per hour
$100.00 per set up
$85.00 per hour
The goals of this core, originally established in 1983, are to provide sophisticated fluorescence-based
analyses and fluorescence-activated cell sorting at reasonable hourly rates. Funding for this facility has
come from institutional and departmental funds, from Pennsylvania Tobacco Settlement Funds, and from
the NIH (Instrumentation for Flow Cytometry/Sorting, PHS RR14851, 4/1/2002-3/31/2003, Dr. B.
Stanley, PI). The services of this core are indispensable for investigators in the field of immunology, and
for running clinical samples. In addition, many cell biology projects use the flow cytometers for
increasingly multiplexed experimental determinations, and the sorter is used for GFP and rare-event cellenrichment and analysis.
Faculty Director: Dr. Todd Schell (tschell@hmc.psu.edu)
Research Project Manager & Flow Cytometry Specialist: Nate Sheaffer (nas2@psu.edu)
Research Technologists: Joe Bednarczyk (jbednarczyk@psu.edu)
Jade Vogel (jvogel@hmc.psu.edu)
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For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/flow-cytometry
•Genome Sciences Core Facility (C2705) (updated 1/16/15)
Nucleic acid processing and analysis: The Facility maintains equipment for nucleic acid extraction
(QIAsymphony and Bullet Blender), sonication (Covaris adaptive focused acoustics
ultrasonicator E-series), quantitation and quality control (Agilent 2100 Bioanalyzer, Nanodrop
Spectrophotometer, Qubit, and access to a Molecular Devices FlexStation 3 UV/Vis/Fluor
spectrophotometer). An LifeTech QuantStudio 12K Flex system with robotic plate handler and
QuantStudio 3D digital PCR system is available for qPCR gene expression and genotyping
services. The Facility provides custom Sanger sequencing of plasmid, PCR and BAC templates.
We can also run fragment analysis assays including footprinting, microsatellite, loss of
heterozygosity (LOH), SSCP, RFLP and genotyping/SNPlex (multiplexed SNP analysis). Cost
for each service is as follows (we allow walk-up and free usage of Nanodrop and Qubit, please
inquire for the usage of FlexStation 3)
Sample prep and PCR service
for base cost -1 sample
RNA isolation
$14.50
DNA isolation
$8.70
Covaris
$8.00
Bioanalyzer (DNA, RNA, protein) (up to 11-12 samples)
$60.00
cDNA synthesis
$1.87
Usage of QuantStudio 12K Flex
$14.00
Kapa library qPCR
$34.00
Digital qPCR
$10.50
GoTaq PCR
$2.15
QIAsymphony service
cost per sample
Whole Blood DNA prep 200
$5.90
Whole Blood DNA prep 400
$7.40
Whole Blood DNA prep 1000
$10.80
DNA prep buffy coat 200
$5.90
DNA prep buffy coat 400
$7.40
RNA prep 400
$6.40
RNA prep 800
$12.00
PAXgene_RNA
$12.00
Saliva DNA prep 1000
$10.80
Sanger sequencing
cost per sample
Custom Sequencing
$6.50
BAC samples
$9.50
Fragment analysis
$2.00
Microarray: The Facility offers Illumina platforms as the main microarray-based procedure for
genotyping and gene expression. The recently acquired iScan system allows high-throughput and
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cost-effective microarray analysis of genotyping, gene expression, copy number analysis,
methylation analysis and LOH studies. Roche and Agilent microarray equipment is also available.
Individual SNP calls can be confirmed by PCR tools described above. Listed below are the costs
of some popular microarrays.
Microarray service
minimum samples required
cost per sample
Infinium Omni 2.5 (16
samples)
16
$214.13
Infinium Omni 2.5 (48
samples)
48
$200.63
Human Omni 5
16
$319.17
Human Omni Express-24 v1.0
48
$149.65
Infinium Methylation
48
$334.99
Human gene expression
24
$98.72
Mouse gene expression
24
$132.65
Next Generation Sequencing platforms: For next generation sequencing projects the Facility offers a
range of workflows depending on investigator needs and number of reads/length of sequence
required for the project. The Facility contains a MiSeq system to perform low output applications
(from 300 Mb to 7.5 Gb in ~4 to 39 hours) and a HiSeq 2500 system, which allows data
collection in both rapid run (up to 90 Gb/flow cell, 2x150 bp, in 40 hours) and high output modes
(up to 300 Gb/flow cell, 2x100 bp, in ~11 days) on either one or two flow cells. The Facility also
maintains a LifeTech Ion Proton DNA sequencer for targeted sequencing and exome sequencing
(10 Gb with sequencing run time of 2-4 hours). An Apollo 324 robotic system supports a variety
of automated library preparation protocols for whole genome, whole exome, ChIP-seq, RNA-seq
and targeted resequencing. Listed below are currently operated services, but we are expanding the
service to meet the needs of each investigator.
NGS library prep service
whole genome
sequencing
whole exome
sequencing
cost per sample*
WGS library prep (minimum input DNA 100 ng)
$50.00
exome (Human) (minimum input DNA 1 ng for intact DNA, under R&D
for FFPE DNA)
$362.00
exome (other species, mouse, bovine, zebrafish)
please inquire
ChIP-seq library prep (minimum input DNA 0.5 ng)
$50.00
Low input ChIP-seq library prep (minimum input DNA 0.05 ng)
$115.33
ERRBS (enhanced reduced representation of bisulfite sequencing) library
prep
$99.40
PolyA RNA seq library prep (strand-specific, minimum input RNA 50 ng)
$69.00
Total RNA seq library prep (rRNA depleted, human, mouse, rat, standard
rRNA-depletion, strand-specific, minimum input RNA 100 ng)
$160.55
Low Input RNA-seq library prep (minimum input RNA 10 pg, or single
cell)
$81.00
Low Input cDNA synthesis (minimum input RNA 10 pg, or single cell)
$71.00
ChIP sequencing
Methylation
sequencing
RNA sequencing
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Degraded Low Input RNA-seq library prep (minimum input RNA 10 ng)
$195.00
Degraded Low Input cDNA synthesis
$145.00
Small RNA seq library prep (minimum input RNA 1 ug)
$97.00
Low input small RNA-seq library prep (minimum input RNA 100 ng)
$115.00
Single cel RNA-seq (up to 96 samples)
please inquire
*QC bioanalyzer run will be added (usually 3 runs ($180) up to 11 samples)
HiSeq 2500
2x: paired end
1x: single end
Sequencing only
Rapid per 2 lanes (need 2 lanes to run the
flow cell)
Rapid per lane (need 2 lanes to run the flow
cell, cBot duo to separate lane 1 and 2)
Output per lane (need 8 lanes to run the flow
cell)
No. of reads (million) per
flow cell (2 lanes run
mixed/run together)
cost per unit
2X100
300
$3,823.32
2X50
300
$3,714.45
1X100
300
$2,406.68
1X50
300
$1,756.73
No. of reads per lane
cost per unit
Reagent kit
Reagent kit
2X100
150
$2,129.66
2X50
150
$2,075.23
1X100
150
$1,421.34
1X50
150
$1,096.37
Reagent kit
No. of reads per lane
cost per unit
2X100
175
$2,145.10
2X50
175
$1,612.11
1X100
175
$1,284.72
1X50
175
$876.62
MiSeq
versions
v2 series
MiSeq reagent kit
No. of reads (million)
cost per unit
2X250
15
$1,271.10
2X150
15
$1,164.39
2X50
15
$955.93
2X150 micro (small
genome)
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$1,006.31
2X150 nano (small
genome, QC)
1
$746.39
10
2X75
25
$1,209.63
2X300
25
$1,820.22
v3 series
Bioinformatics Support: The Penn State College of Medicine has three dedicated High Performance
Computing (HPC) systems with a total of 136 CPU Cores, 768GB RAM and 50TB of local
storage in addition to the 80 TB Storage Area Network (SAN). For the non-human subject
samples, we also have access to the world class High Performance Compute systems at Penn
State University Park, including BioSTAR (512 processor cores, 4 TB of aggregate memory, and
624 TB of disk storage) and CyberSTAR (1,920 cores and 7.68 TB memory, and 650 TB
storage). We conduct sequencing reads alignment, secondary analysis (quantitation, variant
calling, functional annotation, visualization, etc), as well as statistical analysis correlating
genomic with phenotypic information. There is no charge for the consultation and we encourage
each investigator to make an appointment with Director/staffs in advance to secure enough time
for discussion. Director’s availability is found in the 4th tab of below web
site(http://sites.psu.edu/yuka/). Bioinfomatics service will be charged at $60 per hands on
hour. Each project is different, so investigators are encouraged to contact us to estimate the
necessary hours for each project.
The Genome Sciences facility was started in the fall of 2001 entirely with institutional funds, and
additional institutional funds (from Tobacco Settlement Funds) contributed to expansion of this facility in
2003 and 2004, adding the capability to make custom microarrays; adding the capacity to use and
analyze GE-Amersham CodeLink arrays; and adding the capacity to make protein arrays. Funding
through the Penn State Hershey Cancer Institute in 2005 allowed the replacement of older qRT-PCR
instrumentation by adding newer instrumentation for high-throughput analyses, purchase of the Illumina
BeadArray and Bead Express systems in 2008-2009, and purchase of a site license for Ingenuity Pathway
Analysis software in 2010. In 2011-2012, Tobacco Settlement/CURE Funds provided Illumina HiSeq
2500, MiSeq, and Ion Proton whole genome Sequencing capacity, plus an Apollo 324 Robotics system for
library prep. Staffing costs were provided primarily by Tobacco Settlement Funds until 2010, with user
fee chargebacks currently covering staffing costs. This facility greatly enhances the infrastructure for
molecular biology and genetics at the University
Director: Dr. Yuka Imamura (yimamura@hmc.psu.edu)
Research Technologist & Lab Manager: Rob Brucklacher (rbrucklacher@psu.edu)
Research Technologists: Georgina Bixler (gbixler2@hmc.psu.edu)
Elizabeth Conroy (econroy@hmc.psu.edu)
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/gene-expression-analysis.
•Mass Spec & Proteomics Facility (C1732, C1733, C1734, C1735) (updated 5/4/15)
This facility provides multiple separation, digestion, chemical derivatization, mass spec, and database
searching services for proteomic, carbohydrate, oligonucleotide, lipidomics, and small molecule analysis.
Mass Spectrometers: An ABSciex TripleTOF 5600, an ABSciex 5800 MALDI TOF-TOF, an
ABI/MDS/Sciex 4000 QTrap (Hybrid Ion Trap), a Water Synapt HDMS, and a reflectron, delayed
extraction MALDI-TOF mass spectrometer (Voyager DE-PRO, Perseptive Biosystems/Applied
Biosystems) and are available for analyses of proteins, peptides, carbohydrates, lipids,
polymers, small molecules, and oligonucleotides.
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Sample Preparation Services: Multiple sample preparation services are available, including complete
1D and 2D LC separation services (ABI’s Tempo LC-MALDI plate spotter, Eksigent NanoLCUltra 2Dplus system plus cHiPLC nanoflex system and autosampler, and Waters nanoAcquity
UPLC separation systems (plus Advion’s TriVersa NanoMate) for peptide level separations;
Shimadzu UFLC-XR, Waters Acquity UPLC and Agilent 1100 HPLC for small molecule
separations; a Shimadzu HPLC for general HPLC separations, and a Beckman-Coulter PF-2D
system for whole protein level separations and quantitation).
1D and 2D gels: A 12-gel casting and running apparatus for 2D gels (Ettan IPGPhor II and Dalt 12) can
be used by any investigators needing such separations, and standard or DIGE 2D gels can be
analyzed with a GE Typhoon DIGE gel reader and DeCyder software. Subsequent automated spot
excision, proteolytic digestion, and MALDI plate spotting can be done using our Ettan Spot
Picker or our BioMachines/LEAP Technologies 2DiDx spot-cutting robot, and analyzed with our
ABSciex 5800 MALDI TOF-TOF mass spectrometer. Digested samples can also be collected for
further separation and injection using either an ESI (electrospray) or nanospray ionization source
on our 4000 QTrap Hybrid Ion trap, ABSciex TripleTOF 5600, or Waters Synapt HDMS mass
spectrometers.
Sensitivity and types of analyses available: The mass specs can be used for Small Molecule and
Lipidomics Quantitation and Metabolite ID, as well as analyses of complex mixtures of
peptides, for example from proteolytic digests of proteins purified by column chromatography, by
metal affinity chromatography, by SDS-PAGE or 2D gel electrophoresis, or from
PVDF/nitrocellulose membranes, with a microliter of sample material at micromolar
concentrations (equivalent to detection of sub-picomole amounts in a single MALDI spot or ESI
sample injection) sufficient for analysis in most cases – detection and identification of femtomole
and sub-femtomole amounts is possible in many cases. These types of analysis can be used, for
example, to detect and analyze phosphoproteins and other post-translational modifications,
for isozyme analysis, and for identification of unknown proteins, as well as for quantitation of
protein changes (see next section below). Biotransformations/enzyme activity measurements can
be made in some cases without the need for labeled compounds, by direct quantitation of the endproducts, and metabolic flux analyses can be performed by direct quantitation of deuterated
compound and metabolite accumulations. The ion mobility capacities of the Waters Synapt HDMS
can also facilitate characterization of protein and complexes based on mass/size/shape/charge (in
addition to computer modeling/NMR/x-ray/SAXS etc.).
Quantitative Proteomics: Quantitative analyses of differences in protein expression between different
states can be performed at the whole protein level using (1) standard 2D gel analysis comparing
spot-densities on two gels; (2) DIGE gel analysis using a single gel with proteins from different
samples labeled with different fluors; (3) whole protein 2D LC separations and comparing fraction
densities between 2 samples using our Beckman-Coulter PF 2D system; (4) Discovery
Quantitation at the peptide level using differential heavy isotope iTraq or SILAC labeling
followed by gel and/or 2D LC separations, and finally differential relative quantitation on the
TripleTOF 5600 or 4000 QTrap Hybrid Ion Trap instruments using Analyst 1.6 and ProteinPilot
5.0 software, or on the 5800 MALDI TOF-TOF instrument using ProteinPilot 5.0 or GPS Explorer
3.6/Mascot 2.1 software; (5) Targeted Quantitation of up to 100 proteins simultaneously from
complex mixtures using Peptide MRMs on the QTrap 4000 Triple Quadrupole Mass
Spectrometer, or full scan MS/MS modes on the ABSciex TripleTOF 5600 mass spectrometer; or
SWATH/Data-Independent Analysis (DIA) combined ID and Quantitation of hundreds to
thousands of proteins.
Unknown proteins can be further characterized by partial sequence determination of proteolytic
fragments using tandem MS/MS ion fragment analysis. Additional analyses (e.g., partial sequence
determination utilizing Collision-induced Dissociation (CID) fragmentation analysis with our
TripleTOF 5600, 4000 QTrap, 5800 MALDI TOF-TOF, or Synapt HDMS instruments are
available in our facility.
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Software and Bioinformatics: ProteinPilot, Mascot, and Phenyx database matching software is available,
and ProteinPilot includes MIAPE-compliant Protein Grouping software. For large proteomic
datasets, MIAPE-compliant False Discovery Rate estimation is done using decoy-database
searching and the PSPEP algorithms, and further detailed data analysis is done using the
ProteinPilot Descriptive Statistics package. FDR for quantitative results is calculated using inhouse software (PSU_WHATraq) based on the WHATraq program Feb 2014 by Zhou et al. DIAUmpire is used for SWATH analyses (C-C Tsou et al., Nature Methods 12, 258–264 (2015))
Protein Sizing and aggregation state can also be analyzed by the Wyatt DAWN Heleos Light Scattering
Detection instrument located in Dr. John Flanagan’s lab in C5747. This is a shared instrument
purchased for general use; please contact Dr. Flanagan to arrange usage of this instrument
(jflanagan@psu.edu)
A five year plan to create a full-service proteomics facility, using institutional funds made available
through the Tobacco Settlement, was developed in the spring of 2002. The purchase and installation of
the automated tandem MS/MS MALDI-TOF-TOF mass spectrometer (Applied Biosystems 4700
Proteomics Analyzer), in January 2003 was the first stage in this development plan. This was followed
by the acquisition of a BioRad FX Pro Plus 2D gel analysis instrument, an Eksigent 2D Nanoflow LC
separations system and LC Packings/Dionex ProBot automated spotter, an ABI BioCad separation
system,and a Biomachines/Leap Technology 2DiD spot-picking/digestion/sample-spotting robot in
2003 and 2004. In 2005, in addition to 2D gel equipment, a Beckman Coulter PF-2D LC whole protein
separation system was added. In 2006, an MDS/Sciex 4000 QTrap Hybrid Ion Trap Mass Spectrometer
for additional small molecule and post-translational modification analysis capacity was added, as was
a 2nd generation, more sensitive 4800 MALDI TOF-TOF. In 2008, a Waters Synapt HDMS Ion
Mobility Mass Spectrometer was added, and in late 2009 an ABI 5800 MALDI TOF-TOF was added.
Most recently an ABSciex TripleTOF 5600 instrument was added in Fall 2011. Funds for this
instrumentation and staffing of the facility have come from Pennsylvania Tobacco Settlement (CURE)
Funds, other institutional funds, and the Penn State Cancer Institute and National Pharmacogenomics
Center.
Director: Dr. Bruce Stanley (bas12@psu.edu)
Senior Research Technologist & Lab Manager (Proteomics):: Anne Stanley (aes7@psu.edu)
Lab manager (Small Molecules): Dr. Dongxaio Sun (dsun@hmc.psu.edu )
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/proteins-mass-spectometry
•MRI/MRS Facility (updated 10/7/14)
The MRI/MRS Core Facilities are part of the Center for NMR Research, which occupies approximately
6,500 sq ft. in the MRI building behind the Biomedical Research Wing of the College of Medicine. The
MRI Imaging Core provides both Magnetic Resonance Imaging (MRI) and Magnetic Resonance
Spectroscopy (MRS) services, for in vivo & ex vivo spectroscopy and imaging in animals and humans.
These are particularly attractive techniques because they allow the viewing or measurement of closed
internal structures or metabolism of living animals or cells in a completely noninvasive and
nondestructive manner. Magnetic Resonance offers a wide variety of fundamental measurements of
anatomy and physiology. These include detailed anatomical imaging in soft tissues, quantitative
measurements of blood flow or perfusion, fiber tracking of the nerve bundles in the brain, measurement of
metabolism and kinetics in internal organs in situ, volume and staging of tumors, and functional MRI
(fMRI) which can view the effects of specific stimuli on specific brain neurons or regions, allowing one a
means to “see” the brain think.
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Major Equipment:
3.0 Tesla 60 cm bore whole body MRI MAGNETOM TIM Trio (Siemens)
7.0 Tesla 20 cm bore small animal imaging system (Bruker Biospec 70/20as)
128 - Channel high density geodesic portable EEG system capable of full MRI integration.
Additionally, we have an Esys System for functional MR imaging (fMRI) (Invivo Corp, FL, USA). which
includes an LCD visual display, audio system, button response unit and software (E-Prime) for paradigm
creation, patient management, protocol planning, precise delivery of brain stimulation, and behavioral
analysis. Furthermore, we also have an Agilent E4991A RF Impedance material analyzer, an HP 4195A
Network/Specturm Analyzer, an HP 8452A Diode Array Spectrophotometer, an Orion EA920 pH/ion
analyzer, balances, a LeCroy 9450A 300 MHz dual digital oscilloscope, 2 analog oscilloscopes, 2 Morris
Model 505 RF Sweepers, and an HP 4263A LCR Meter.
MRI Methods (What MRI can do for your research)
MRI allows for in vivo imaging studies for humans and animals. In addition to the conventional
anatomical imaging methods, CNMRR provides the new MRI modalities for advanced research:
Functional MRI (fMRI)
Quantitative parametric mapping
Quantitative morphological measurement
Diffusion Tensor Imaging (DTI)
Magnetic Resonance Spectroscopy (MRS)
Current Fees: Rates for instrument use are:
7 Tesla Animal Imaging - $157 per hour
3 Tesla Human Imaging - $452 per hour.
Faculty Director: Dr. Qing Yang (qyang@hmc.psu.edu)
Research Project Manager: Jeff Vesek (jvesek@hmc.psu.edu)
Research Technologist: Patti Miller (pmiller5@hmc.psu.edu)
•Solution Phase NMR Facility (C2818A) (updated 9/13/10)
The 500 MHz and 600 MHz Bruker NMRs in this facility provide high field strength NMR instruments
necessary for a range of projects including macromolecular structure determination, organic chemistry
and metabolomics. The 500 MHz instrument has several probes, including a broadband probe for direct
detection of nuclei with frequencies ranging from Nitrogen to Phosphorous with proton decoupling, a
dual fluorine and proton probe with detection and decoupling for either channel, a triple resonance
cryoprobe (proton, carbon, nitrogen) with high sensitivity for proton and carbon, and a high-resolution
magic angle spinning triple resonance (proton, carbon, phosphorous) probe for measurements involving
solids and intact tissue samples. The 600 MHz instrument has a triple resonance cryoprobe (proton,
carbon, nitrogen) with the greatest sensitivity for proton and carbon, and a 1 mm triple resonance (proton,
carbon, nitrogen) microprobe for sample volumes as small as 5 µl. Both instruments have cryofit
accessories which use a robot to deliver multiple samples to the cryoprobe, allowing for a considerable
degree of automation.
Fees: $30 per hour for operator assisted use; $6 per hour for self-service use (user must be trained and
approved/certified to be allowed to run samples without operator).
Director: Dr. Fang Tian (ftian@psu.edu) 717-531-6775
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Project Manager: Dr. Jyh Ming Lin (jml47@psu.edu)
717-531-3612 (Office), 717-531-5340 (NMR Lab)
•Transgenic Mouse Facility (Department of Comparative Medicine, ARF 173,136, 144) (updated
1/23/13)
This facility can provide injection and breeding services for development of knockout or transgenic
mouse lines. To create such transgenic or knockout lines, the core lab performs microinjection of purified
DNA into the pronucleus of fertilized mouse eggs on two separate injection days. Eggs are then returned
to pseudopregnant females, and founder lines established. The core lab harvests eggs, injects them on
two separate injection days, reimplants eggs, and coordinates litter stocks up to the establishment of a
founder line. Procedures for targeted gene disruption using embryonic stem cells have also been
developed, and over 80 transgenic and knockout mouse lines have been created
Costs for Transgenic Mouse Production depend on the strain of mouse used as follows:
Set-up Fee
Mouse Strain
Injection Fee
Total Price
(non-refundable)
B6D2F1
$600.00
$2405.00
$3005.00
C57BL/6
$800.00
$2895.00
$3695.00
FVB
$600.00
$2840.00
$3440.00
Investigator Provided* $200.00
$1825.00
$2025.00
*Mouse strain provided by the investigator: Male and female mice needed for production of fertilized
embryos for injection are supplied by the investigator. These mice must be kept under the investigator’s
mouse protocol and be proven pathogen free before being moved into the Transgenic mouse room. No
guarantee applies to these mice.
Knock-out Mice:
# of Injections
1 Injection Day
3 Injection Days
5 Injection Days
Set-up Fee
(non-refundable)
$500.00
$1500.00
$2500.00
Injection Fee
Total Price
$1120.00
$3360.00
$5600.00
$1620.00
$4860.00
$8100.00
Rederivation Fee
Total Price
$1365.00
$1665.00
Rederivations:
Mice
1 Line
Set-up Fee
(non-refundable)
$300.00
Mouse Sperm Cryopreservation:
3 Strain kit: $3750.00 plus $28.00 per hour. (Normally about 1 hour per strain)
6 Strain kit: $7025.00 plus $28.00 per hour.
9 Strain kit: $10,150.00 plus $28.00 per hour.
Investigator Responsibilities: The individual investigator is responsible for providing ultrapure DNA
fragments or Embryonic Stem Cells for ES work, and for performing appropriate Southern blot or other
validated analyses on founders and their progeny to establish integration of the transgene.
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The purpose of the Transgenic Core Lab is to develop transgenic and targeted gene disruption mouse
models for use in biomedical research, thus providing powerful approaches for the study of gene function
in relation to normal health and disease. As with other cores, centralizing the basic microinjection and
stem cell technologies in a common core lab avoids unnecessary and expensive duplication of efforts, and
more importantly, experience with similar facilities at other institutions strongly indicates that the
efficiency and success rates of transgenic experiments are greatly improved in a core facility where the
procedures are conducted routinely. The Transgenic Core Facility is housed in the Animal Research
Farm in dedicated core space proximate to the animal care facilities. Establishment of this facility was
funded by an NIH grant (Drs. Billingsley, Levenson, and Pegg) and by a commitment of institutional
resources, which provided funds for salary and fringe benefits for a core lab technician and equipment
for microinjections, a Leica microscope with dual micromanipulators, a Sutter programmable pipette
puller, a Narashige microforge, an Olympus dissecting stereoscope, a laminar flow hood, and one
incubator.
Director: Dr. Ronald Wilson (rpw4@psu.edu)
Lab Manager: Alane Seidel (aks2@psu.edu)
(Faculty Advisory Committee: Dr. Ron Wilson, Dr. Rob Bonneau)
For the most up-to-date information, see the facility’s web page and links at
http://infonet.hmc.psu.edu/animal-resources-program/services/Transgenic core home.htm.
•Bioluminescent/Fluorescent Imaging Facility (updated 1/23/13)
The Bioluminescent/Fluorescent Imaging Core Facility provides a non-invasive way to track and
quantify localized bioluminescent and fluorescent signals. This type of analysis is particularly useful to
study activities of gene promoters in cells or in whole animals, for example during normal development,
after drug exposure or toxic insult, or during tumor growth. Promoter analyses typically involve
expressing luciferase under the control of a gene promoter of interest and incorporating this construct into
cells in culture. Alternatively, transgene-derived luciferase expression can be achieved in transgenic
mouse models that utilize tissue specific promoters. After injection of the luciferin substrate, areas where
the transgene is being expressed can be clearly measured and differences in tissue or regional expression
can be quantitated. Alternatively, fluorescent proteins expressed in transgenic animals or transfected
human cell xenografts can also be detected. Additional applications include the localization and
quantification of reagents or cells labeled with fluorescent molecules, such as quantum dots, after
injection into the animal. Because the measurements do not involve sacrificing the animal, serial
measurements or time courses can be obtained. This strategy allows more accurate data collection and
increased efficiency by utilizing fewer animals due to reduced inter-animal variability.
https://drive.google.com/ - my-drive
Services offered:
 Non-invasive whole animal imaging of bioluminescent/fluorescent signals in animals (transgene
expression, developmental patterns, drug or treatment effect time courses, tumor staging, etc.)
 Imaging of bioluminescence or fluorescence from cells in culture
Major Equipment:
 Xenogen IVIS 50 Imaging System in the Animal Research Barrier Facility
 Remote analysis workstation for image processing
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Director: TBA
For the most up-to-date information, see the facility’s web page and links at
http://med.psu.edu/web/core/whole-animal-luminescent-imaging-services
•Tecan EVO Robotics/Liquid Handling (C2706) (updated 7/15/14)
The Penn State College of Medicine Research Core Facilities include a sophisticated Robotics Platform
for automated sample preparation, dilution, mixing, and aliquoting; our Tecan Freedom EVO system.
The Tecan robot is located in the Institute for Personalized Medicine suite in room C2706, and is operated
under oversight provided by funding from the Penn State Hershey Cancer Institute. For the most up-todate information, see the facility’s web page and links at http://med.psu.edu/web/core/tecan-liquidhandline-robotics
Capacities/Services Available:




Useful for high-throughput pipetting, particularly when the project involves performing the same
protocol multiple times
Primary uses so far have been running multiple 96 or 384 well plates for various protocols (PCR,
ELISA, dilutions, etc)
8 channel arm – From 1-8 pipettes at a time to distribute liquid, useful for pipetting from troughs,
test tubes, mircofuge tubes, or 8 at a time along a 96 or 384 well plate
96 channel arm – All 96 tips pipette at one time, useful for pipetting from 96 well or 384 well
plates to 96 well or 384 well plates
Examples of Completed Protocols:





96 and 384 well plate transfers and dilutions
PCR plate set-up (real-time gene expression or genotyping)
ELISA plate set-up (from tubes), incubation, and wash
DNA Quantification protocols (Picogreen)
Drug interactions with live cells
Services and Scheduling
List of Services
Fees
Hourly Use
$26.67 per hour (for all protocols,
non-commercial rate)
Scientific Consultation
Above hourly charge applies only if
Tecan use is scheduled.
To schedule an appointment to use the Tecan robot, please contact:
Director: Dr. Carla J. Gallagher (cgallagher@hmc.psu.edu) Phone: 717-531-2973
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