Abstract Book
State level Conference on
‘Microbiology in 21st Century’
25th – 26th February, 2011
Dr. Gajanan R. Ekbote
Chief Organizer and
Chairman, Business Council, Progressive Education Society, Pune 5.
Dr. Rajendra. S. Zunjarrao
Convener and
Principal, Modern College of Arts, Science and Commerce, Pune 5.
Dr. (Mrs.) Shilpa S. Mujumdar
Organizing Secretary and Head, Department of Microbiology
Organized By
Department of Microbiology
Modern College of Arts, Science and Commerce
Shivajinagar, Pune 5.
Under Quality Improvement Programme of University of Pune
State Level Conference on ‘Microbology in 21st Century’
Mission statement
“To Create
Multidisciplinary Best Citizens
To Suit
Local, National and International Needs
Having
Scientific Temperament,
Moral and Ethical Values
and
Multifaceted, Proactive Personality
By
Providing Excellent Education”
II
1
State Level Conference on
‘Microbiology in 21st Century’
25th – 26 th February, 2011
Organized by
Department of Microbiology
Modern College of Arts, Science & Commerce,
Shivajinagar, Pune, 411005, Maharashtra, India.
th
Conference Schedule - Friday, 25 February 2011
Inaugural Function
10.00 am-11.00 am
Registration and Refreshment
11.00 am-12.00 pm
Inauguration
12.00 pm-12.45 pm
12.45 pm-01.30 pm
• DR. GAJANAN R. EKBOTE, Chairman, Business Council, P. E. Society, Pune
• DR. RAMESH S. PARANJAPE, Director, NARI, Pune
• PROF. MRS. JYOTSNA. G. EKBOTE, Chairman, LMC (Junior College), MCASC, Pune
• DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Pune
• DR. ARVIND K. PANDE, Prof-In-charge, Department of Biotechnology and Microbiology.
DR. RAMESH S. PARANJAPE, Key Note Speaker
Director,
National AIDS Research Institute, Pune
Lunch
Session I
01.30 pm-02.00 pm
02.00 pm-02.45 pm
Lectures
DR. PRASHANT K. DHAKEPHALKAR, Chairperson
Scientist ‘E’, Agharkar Research Institute, Pune
DR. S. MOHAN KARUPPAYIL, Invited Speaker
Topic:” Prokaryotes and Eukaryotes: Do they talk to each other?”
Professor and Director, School of Life Sciences, S.R.T.M.University, Nanded
02.45 pm-03.30 pm
DR. PRAKASH R. THORAT, Invited Speaker
Topic:”Decolourization and degradation of textile dyes by microorganisms”
Executive member, AMI; HOD, Microbiology, Shri Shivaji Mahavidyalaya, Barshi.
03.30 pm-03.40 pm
03.40 pm-03.50 pm
03.50 pm-04.00 pm
04.00 pm-04.10 pm
04.10 pm-04.20 pm
04.20 pm-04.30 pm
04.30 pm-04.40 pm
04.40 pm-05.00 pm
Oral Presentations
OP 01
Candida glabrata infection of the nasal septum – an unusual case report.
Dr. Roma A. Chougale
MBBS, MD, Lecturer in Microbiology, D. Y. Patil College, Kolhapur.
OP 02
Prevalence of methicillin resistant Staphylococcus aureus in D.Y.Patil hospital and Research centre.
Dr. Reena A.Dighe
MBBS, DCP, MD, Department of Microbiology,D. Y. Patil Medical College, Kolhapur.
OP 03
Bacteriological quality of water, sanitation interventions and health with special reference to diarrhoea.
Mr. Anil M. Garode
Department of Microbiology, Shri. Shivaji Science and Arts College, Chikhli, Buldhana.
OP 04
Screening of fungal isolates for lignolytic enzyme, manganese peroxides
Madhuri A. Udata, Joshi A. J., Dr. Mahendra K. Ranjekar.
Department of Microbiology, Dayanand Science College, Latur.
OP 05
Biotransformation of Textile Azo Dyes by Aerobic Bacteria.
Ravi V. Kale and Prakash R. Thorat.
P.G.Department of Microbiology and Research Center, Shri Shivaji Mahavidyalaya, Barshi, Solapur.
OP 06
A cost-effective protocol for cultivation, microbial and biochemical analysis and high yielding nutritious variety of
mushrooms.
Supriya V. Suryawanshi, Bhagyashree V. More, Nilesh P. Anandwani, Jayesh A. Patil, Department of Microbiology,
PSGVPM’s, ASC College, Shahada, 425409, Nandurbar.
OP 07
Biosurfactant production by a marine strain of Pseudomonas.
Vijendra A. Kavatalkar, Smita S. Bhuyan, Peehu S. Pardeshi, Prof. Balu A. Chopade.
Department of Microbiology & Institute of Bioinformatics and Biotechnology, University of Pune, Pune.
Tea
2
Conference Schedule – Saturday, 26th February 2011
Session II
Lectures
10.00 am-10.30 am
10.30 am-11.00 am
Refreshment
DR. DIGAMBAR V. GOKHALE, Chairperson
Scientist ‘F’, National Chemical Laboratory, Pune
11.00 am-11.45 am
PROF. ARVIND M. DESHMUKH, Invited Speaker
Topic:”Microbial solubilization of metals from waste printed circuit boards”
11.45 am-12.30 pm
PROF. SUDHIR B. CHINCHOLKAR, Invited Speaker
Topic:”Recent Trends in Microbial Control of Phytopathogens”
Head, Department of Microbiology, BAMU, Osmanabad.
Director, BCUD, North Maharashtra University, Jalgaon
12.30 pm-12.40 pm
12.40 pm-12.50 pm
12.50 pm-01.00 pm
01.00 pm-01.10 pm
01.10 pm-01.20 pm
1.20 pm-01.30 pm
01.30 pm-02.00 pm
Oral Presentations
OP 08
Antiplasmid activity of herbal extracts on Methicillin Resistant Staphylococcus aureus
Avinash D. Bholay, Anita N. Katkade, Kaveri S. Palekar.
Department of Microbiology, K. T. H. M. College, Nashik
OP 09
Study of extended spectrum beta lactamase (ESBL) producing Gram negative bacilli in family Enterobacteriaceae.
Dr.Vishwashanti. S. Vatkar, Dr.P.G.Shadija, Dr.S.J.Ghosh
Department of Microbiology, Dr.D.Y.Patil Medical College, Kasaba Bavada, Kolhapur.
OP 10
Microbial Bioaugmentation for treatment of industrial effluents in Common Effluent Treatment Plant (CETP)
Snehal B. Bari, Nilesh A. Sonune, Amit R. Sinnarkar, Dr.Seema S.Sarnaik, G.K.Wagh, Dr.Pradnya P.Kanekar
Microbial Sciences Division, MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune.
OP 11
Isolation of Rhizobium and Optimization of Growth Conditions for Development of Biofertilizer for Trigonella
foenumgraecum (Fenugreek).
Aasawari S. Pawar and Rajendra Choure.
Department of Microbiology, The Institute of Science, Mumbai.
OP 12
Synthesis and characterization of ZnO nanoparticles and their application on textiles as antimicrobial agents and UV
absorbers.
Rutuja.R.Phatate, Rebecca.S.Thombre and Sonal.P.Bathija.
Department of Biotechnology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune.
OP 13
Comparative study on the production of Prodigiosin by Serratia marcescens using various crude fatty acid sources and its
applications.
Pankaj .S. Picha, Sheetal .P. Pardeshi
Department of Microbiology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune.
Lunch
Session III
02.00 pm-03.00 pm
Poster Presentation
Session IV
03.00 pm-04.00 pm
04.00 pm-04.10 pm
Panel Discussion
•
DR. BALU A. CHOPADE, Director, Institute of Bioinformatics and Biotechnology, Pune
•
DR. RAJNEESH B. VAIDYA, HOD, Microbiology, The Institute of Science, Mumbai.
•
DR. BALU P. KAPADNIS, HOD, Microbiology, University of Pune, Pune.
•
DR. RENU BHARADWAJ, Dean, B.J.Medical College, Pune.
•
DR. PRAKASH R. THORAT, Executive member, AMI; HOD, Microbiology, Shri Shivaji Mahavidyalaya, Barshi.
•
DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Shivajinagar, Pune.
•
DR. ARVIND K. PANDE, Prof. In Charge, Department of Microbiology and Biotechnology
Tea
04.10 pm-04.40 pm
Valedictory function
•
DR. GAJANAN R. EKBOTE, Chairman, Business Council, P. E. Society, Shivajinagar, Pune.
•
DR. RAMCHANDRA V. GADRE, Scientist, National Chemical Laboratory, Pune
•
PROF. MRS. JYOSTNA. G. EKBOTE, Chairman, LMC Junior College, MCASC, Shivajinagar, Pune.
•
DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Shivajinagar , Pune
•
DR. ARVIND K. PANDE, Prof. In Charge, Department of Microbiology and Biotechnology, MCASC, Shivajinagar , Pune
•
DR. SHILPA S. MUJUMDAR, Head, Department of Microbiology, MCASC, Shivajinagar, Pune.
04.40 pm-05.10 pm
Certificate Distribution
State Levell Conference on
o ‘Microbologgy in 21st Centtury’
Messagge from Exe
ecutive me
ember, AM
MI
Dr. Thoraat P. R.
Executive Member,
Associationn of Microbiollogist of India
Head,
P.G. Deparrtment of Micrrobiology and Research
R
Centeer,
Shri. Shivaaji Mahavidyallaya, Barshi 413411
Member BOS
B
in Microb
biology
Faculty of Science,
Solapur Unniversity,
Solapur, Maharashtra
M
“IIt gives me imm
mense pleasuree to know that Modern Collegge of Arts, Scieence
and Comm
merce, Shivajiinagar, Pune-55 is organizinng a State levvel Conferencee on
‘Microbioology in 21st Ceentury’ from 25
2 th – 26th Feb. 2011.
M
Microbiology
iss one of the fasstest growing knowledge
k
baseed sector and itt has
numerous advantages in terms of reseaarch and development. The research done and
ongoing reesearch in this field has usshered in a reevolution in thhe field of heealth,
medicine and pharmaceeutical, food, biotech indusstries, thus paaving the wayy to
improve annd increase thee productivity in the country.
Thhe scope of Microbiology
M
is diverse and it covers areaas as different and
distinctive as Pharmaaceuticals, Agricultural
A
M
Molecular
and Environmeental
mely importannt, therefore to provide qualitty exposure to new
Microbioloogy. It is extrem
generation of students and to acquaintt them with alll the facets off this multifacceted
A
area of stuudy. It is thus extremely commendable thhat the Modernn College of Arts,
Science annd Commerce, Shivajinagar, Pune
P
05 is hosting the State level
l
conferencce of
its kind in the area of Miccrobiology in Pune
P
region.
n 21st Centuryy” will providee the
I am thus quite sure that “Miicrobiology in
perfect plaatform to bring
g together studeents, researcheers and subjectt experts to disscuss
the emerging area of Miccrobiology for benefit
b
of all liiving beings.
I convey my best wishes to the organnizers for a great successs of
‘Microbioology in 21st Ceentury’ conferrence.”
T
Dr. P. R. Thorat
Member Central
C
Executiv
ve Council,
Associationn of Microbiollogists of Indiaa
III
State Levell Conference on
o ‘Microbologgy in 21st Centtury’
Messagge from Dirrector, IBB
B, University of Punee
Professorr B. A. Chopade
Director,
Institute off Bioinformaticcs and Biotechnnology,
University of Pune
Ganeshkhiind, Pune 07
m pleasure too note that thhe department of Microbiollogy,
“IIt is indeed my
Modern College of Artss, Science and Commerce, Pune
P
5 is organnizing State Level
L
st
th
th
C
on 255 and 26 Feebruary 2011. The
Conferencee on “Microbiology in 21 Century”
Pune city, historically en
nriched with cuultural heritagee is also a majoor educational hub
s
an imporrtant
in the couuntry; it is theerefore an apppropriate locaation to host such
conferencee.
Thhe subject Miccrobiology has great relevancce, connotationns and concernss not
only to microbiologists but
b to all the walks
w
of humann life through its
i immense poower
o microbial biotechnology.
b
Microbiologyy has made grreat impact onn all
in terms of
facets of human
h
life, naay it be foods and nutrition,, health and wellness,
w
indusstrial
chemicals and materials,, energy, agricculture or ecoloogy and enviroonment. Microobial
d as one of thee most precious national resoources, on par with
diversity iss being viewed
mineral orr fossil fuel resources. New engineeredd bacteria usseful in indusstrial
applicationns are emerging
g from the new
w discipline of synthetic bioloogy.
M
Microbiology
an
nd Microbioloogist in particuular play an im
mportant role inn the
developmeent of scientific advancem
ment and new
w vista in moodern researchh in
biological sciences. It is the time to recollect the immense contributionss of
microbioloogists that made our life comffortable and heealthy.
I wish that all the delegates of the conferrence to get maximum
m
bennefits
on in the confeerence. I send my best wishees and greetinggs to
through their participatio
t event good luck and grand
g
all the parrticipants and the organizerrs and wish the
success.”
Professor B.A. Chopadee
Director,
Institute off Bioinformaticcs and Biotechnnology,
University of Pune
Ganeshkhiind, Pune 07
IV
State Level Conference on ‘Microbology in 21st Century’
Message from Head, Microbiology, University of Pune
Professor B. P. Kapadnis
Head,
Department of Microbiology,
University of Pune,
Ganeshkhind, Pune 07
“I am happy to know that the Department of Microbiology, Modern College
of Arts, Science and Commerce, Pune 05, is organizing State Level Conference
entitled “Microbiology in 21st Century” on 25th and 26th February 2011.
In today’s Modern world, Microbiology is considered a vibrant and exciting
field with many applications and the new and exciting discoveries been made in
microbial ecology, molecular genetics, space microbiology etc. With the changing
faces of microbes, it is mandatory for us to refine our approaches towards treating the
infectious diseases. Genetically tailored medicine is picking up pace and that really
gives us a ray of hope in combating the problem of multidrug resistant organisms
which is the need of the hour. This conference will present multitude of subjects with
a unique blend of modern research in microbiology.
I am certain that this conference will prove to be the perfect platform for the
professionals to greatly benefit from the exchange of ideas and also the debates and
deliberations related to the explorations of an integrated approach to microbiology.
I wish all the best and success for this conference.”
Professor B. P. Kapadnis
Department of Microbiology,
University of Pune,
Ganeshkhind, Pune 07
V
State Levell Conference on
o ‘Microbologgy in 21st Centtury’
Chairmaan’s Messaage
Dr. G. R. Ekbote
E
Chairman
n,
Business Council,
C
Progressive Education So
ociety
Shivajinaggr, Pune 5.
Chief Orgganizer,
‘State leveel Conferencee on
Microbiollogy in 21st Century’
C
25th - 26th February 201
11
“P
Progressive ed
ducation Societty is one of thee leading educcational instituttions
in Maharashtra. P. E. So
ociety was fouunded by a weell-known deddicated teacher late
Shankarraoo Kanitkar alo
ong with his coolleagues in yeear 1934. The team of dediccated
teachers with
w
their unttiring efforts and sacrifice built up repputation of alll its
institutionss over a period
d of time. Thee school, colleeges, managem
ment and compputer
institutes run
r by the sociiety have earneed a reputationn as institutionn imparting quuality
education all over Maharrashtra. P. E. Society
S
runs 56 educational institutions, which
w
include pree-Primary, Prim
mary, Secondary, Higher Seccondary Schooll and Arts, Science
and Comm
merce Colleges, Engineeringg College, Coollege of Pharmacy, Institutte of
Managemeent, Institute of
o Computer Science,
S
Inforrmation Technnology Centre and
Law Collegge etc.
Thhe Modern Co
ollege of Arts, Science and Commerce,
C
Shhivajinagar, Puune 5
was foundeed by Progresssive Education Society, in 19970. The collegges affiliated too the
University of Pune. The college providdes various accademic amenities so as to attain
a
a Master Deegrees in the field of Arts, Science, Com
mmerce, Compputer
Bachelor and
Science, Computer
C
Ap
pplication and Biotechnologgy. The colleege is a repputed
educationaal institution, which
w
has beeen known for producing ouutstanding studdents
who take on
o different carreers, as per thheir academic merit
m
successfu
fully in the socciety.
University of Pune has aw
warded “Best College
C
Award” to the collegge.
a
deveelopment of the students but also
Thhe college not only ensures academic
provides thhem with opportunities to prove
p
themselvves by means of extra-curriccular
and co-currricular activitiies. Moreover,, in the field of
o sports, the college
c
has maade a
name for itself.
i
So far 28
2 students off the college have
h
won presttigious ‘Shri. Shiv
Chhatrapatti Award’. Th
he College has a well-equippped gymnasium by whichh the
students arre benefited. The Arts circle of the college works hard foor the promotioon of
cultural acctivities. The Career
C
Guidancce Center makkes its presencee felt by proviiding
necessary information
i
an
nd guidance to the students as
a and when required. The record
of achievem
ments of the co
ollege clearly indicates
i
that the
t college hass firmly established
itself as ‘Modern’ an
nd ‘Progressivve’ educationnal institution.. The College is
B
Varrious educationnal authorities have
h
accredited with ‘A’ gradee by NAAC, Bangalore.
VI
State Level Conference on ‘Microbology in 21st Century’
noted the achievements and reputation of the college. The Govt. of Maharashtra and
University of Pune has recently accorded the sanction to increase the intake capacity
of the M.Sc. course. The young and highly qualified team of faculty members spares
no efforts to keep the students well informed and in updating their knowledge. The
students are also reciprocating by their high achievements in academic performance.
A large number of students are getting excellent placements in different part of the
country & also abroad. Every year several students are also selected in reputed foreign
universities for higher studies.
Concentrated research and development is required on various micro based
technologies that can be utilized to increase crop production and utilize agro waste,
manage abiotic stress drug design, for biocontrol of important insect pests and in post
harvest technologies
I am certain that the conference will prove to the perfect platform for the
professionals to greatly benefit from the exchange of ideas and also, the debates and
deliberations related to explorations of integrated approach to Microbiology. I also
hope that there will be sufficient knowledge and direction for the young and budding
scientists also.
I congratulate the principal of modern college Dr. Rajendra S. Zunjarrao, Dr.
Shilpa S. Mujumdar, HOD, Department of Microbiology and organizing team for
organizing this conference and wish them a grand success.”
Dr. G. R. Ekbote
Chairman,
Business Council,
Progressive Education Society
Shivajinagr, Pune 5.
VII
State Levell Conference on
o ‘Microbologgy in 21st Centtury’
Principaal’s Message
Dr. Rajen
ndra S. Zunjarrao
Principall,
Progressivve Education Society’s
Modern College
C
of Artts, Science annd Commercee,
Shivajinaggar, Pune 5.
Convenerr,
‘State leveel Conferencee on
Microbiollogy in 21st Century’
C
25th - 26th February 201
11
“W
We are happ
py to welcom
me you to thhe State leveel Conferencee on
‘Microbiollogy in 21st Century’ organiized by Deparrtment of Micrrobiology, Modern
College off Arts, Science and Commerce, Pune 05.
Siince 1985, P. E.
E Society has been making pioneering
p
effoorts in establishing
higher andd technical ed
ducational insttitutions underr the dynamicc leadership of its
Chairman, Dr. G. R. Ekbote, a well known surgeeon and membber of the Sennate,
Academic Council of th
he University of Pune. He has been receently appointed as
U.G.C. Noominee on Univ
versity of Heallth sciences, West
W Bengal annd also on Natiional
Institute off Technology, Surathkhal, Karnataka.
K
Thee institution off P. E. Societyy are
progressingg remarkably and
a making thheir mark in thhe field of education. It is inn this
context thaat the era from
m 1986 till todaay is characterized as era of exponential
e
groowth
and academ
mic developmeent.
Prrogressive Ed
ducation Socieety’s Modern College of Arts
A
Science and
Commercee, Shivajinagarr, Pune 05, waas established in the year 19970. At presentt we
have approoximately 8,00
00 students. We
W run coursess in arts, comm
mence and science
including Biotechnology
y, Microbioloogy, Computerr Science etcc. The college is
reaccrediteed by NAAC, Bangalore witth ‘A’ grade sttatus. The college is well knnown
for its acaddemic excellen
nce. Many passt students of our
o college haave excelled inn the
field of accademic activitties, sports andd cultural actiivities. The coollege has receeived
‘Best College Award’ off University off Pune in year 2008.
2
Thhe subject Miccrobiology hass always remainned fascinatingg. Microorganiisms
are thoughht to be the orrigin of life and
a also big factories
fa
produucing thousandds of
biochemicaal compounds. The whole mechanism
m
off life processees is illustratedd by
these tiny organisms. They
T
are versaatile in adaptiing themselvees to any kindd of
environmeent. They projeect themselves to be both useeful and harmfuul to life. All these
t
unique feaatures of microo
organisms willl be reveled in this conferencce. The title of the
conferencee” Microbiolog
gy in 21st Centtury” speaks about
a
the glorioous progress made
m
in various branches of microbiology
m
toouching many aspects of life. The topics off the
ortunity to scientists,
s
teaachers, studennts, industriallists,
conferencee offer oppo
environmeentalists, agricu
ulturists and eccologists to preesent their conttributions madee for
the betterm
ment of human life.
O college haas maintained tradition of organizing
Our
o
Nattional/Internatiional
Level Connferences, Work
kshops, Seminnars and Symposiums. Althouugh, Microbiology
is a basic science, it haas played majoor role in the development various aspectts of
VIII
State Level Conference on ‘Microbology in 21st Century’
science. With this notion in mind we had submitted proposal to the University of
Pune to organize State level conference on ‘Microbiology in 21st Century’ under its
Quality Improvement Programme. We are very glad to communicate you that, in
response to our Proposal University of Pune has given approval and consent to
organize the International Conference on the said topic.
As it is state level event we have invited and expecting the participation of
Resource Persons, Subject Experts, Scientists, Eminent Personalities from the
academic field and industry, Faculties and Students from the various districts in
Maharashtra. On this occasion I convey my warm greetings and felicitations to all the
delegates and wish them a scintillating conference.”
Dr. Rajendra S. Zunjarrao
Principal,
Progressive Education Society’s
Modern College of Arts, Science and Commerce,
Shivajinagar, Pune 5.
IX
State Level Conference on ‘Microbology in 21st Century’
Committees for State Level Conference
“Microbiology in 21st Century”
25th and 26th February 2011
Organizing committee
Dr. G. R. Ekbote, Chief Organizer and Chairman, P.E. Society.
Dr. R. S. Zunjarrao, Principal and Convener
Prof. A. G. Gosavi, Visitor, MCASC, Pune 5
Dr. A. K. Pande, Prof. In‐Charge, Microbiology
Dr. (Mrs.) S. S. Mujumdar, Organizing Secretary and HOD, Microbiology
Prof. A. M. Bhalerao, Professor, Dept. of Zoology
Prof. S. S. Thengadi, Professor, Dept. of Physics.
Prof. Y. Thipse, Asst. Prof. Dept. of Electronics
Ms. S. S. Bhuyan, Asst. Prof, Microbiology
Mr. V. N. Bobade, Asst. Prof., Microbiology
Ms. S. P. Pardeshi, Asst. Prof., Microbiology
Ms. S. P. Bashetti, Asst. Prof, Microbiology
Mrs. A. A. Devale, Asst. Prof., Microbiology
Ms. M. J. Amrutkar, Asst. Prof, Microbiology
Mrs. R. S. Sawant, Asst. Prof, Microbiology
Advisory Committee
Dr. G. R. Ekbote, Chairman, P.E. Society.
Dr. P.S. Chirputkar, Chairman, LMC (Senior College), MCASC.
Dr. A. K. Pande, Prof.‐In‐Charge, Dept. of Biotechnology and Microbiology, MCASC, Pune 5.
Prof. Mrs. J. G. Ekbote, Chairperson, LMC (Junior College), MCASC.
Dr. R. S. Zunjarrao, Principal, MCASC, Pune 5.
Prof. A. G. Gosavi, Visitor, MCASC, Pune 5.
Prof. S. S. Deshmukh, Vice‐Principal, MCASC, Pune 5.
Prof. S. Choudhari, Vice‐Principal, MCASC, Pune 5
X
State Level Conference on ‘Microbology in 21st Century’
Scientific Advisory Committee
Prof. Dr. B. A. Chopade, Director, IBB, University of Pune
Prof. Dr. B. P. Kapdnis, Head, Dept. of Microbiology, University of Pune.
Prof. Dr. R. L. Deopurkar, Professor,
Dept. of Microbiology, University of Pune.
Dr. P. K. Dhakephalkar, Scientist ‘E’, ARI, Pune
Prof. Dr. Sankunny M. Karuppayil, Director, School of Life Sciences, S. R. T. M.
University, Nanded
Dr. D. V. Gokhale, Scientist ‘F’, Scientist In charge, ICIM, NCL, Pune
Prof. A. M. Deshmukh, Head, Department of Microbiology, BAMU, Osmanabad
Prof. S. B. Chincholkar,
Director, BCUD, North Maharashtra University, Jalgaon
Dr. P. R. Thorat, Head, Dept. of Microbiology, Shri Shivaji Mahavidyalay,
Barshi
Dr. H. V. Ghate, Head, Dept. of Zoology, MCASC.
Editorial Committee
Chief Editor:
Dr. R. S. Zunjarrao, Principal
Advisor:
Dr. A. K. Pande
Prof. S. S. Deshmukh, Vice‐Principal, MCASC, Pune 05.
Members:
Dr. (Mrs.) S. S. Mujumdar
Mr. V. N. Bobade
Ms. S. S. Bhuyan
Travel and Accommodation Committee
Advisor:
Prof. A. G. Gosavi, Visitor
Members:
Dr. (Mrs.) S. S. Mujumdar
Mr. V. N. Bobade
Mr. P. S. Warhade
Mr. A. A. katkar
Mr. V. M. Daundkar
Ms M. J. Amrutkar
Mr. P. Sabale
XI
State Level Conference on ‘Microbology in 21st Century’
Technical Assistance Committee
Advisor:
Prof. S S. Deshmukh
Prof. A. Sathe,
Members:
Mr. V. N. Bobade
Ms. S. P. Pardeshi
Ms. Gitanjali Litake
Ms. A. V. Jagdale
Mr. A. A. Katkar
Mr. V. M. Daundkar
Mrs. J. J. Dunde
Mr. R. Shawalkar
Mr. Manik Jadhav
Registration Committee
Advisor:
Mr. R. P. Kale, Registrar
Members:
Mrs. A. A. Devale
Ms. S. P. Bashetti
Mrs. R. S. Sawant
Mr. R. Dabhade
Ms. R. Thombare
Mr. V. Lad
Mrs. S. Patwardhan
Mr. V. M. Daundkar
Ms. A. V. Jagdale
Mr. Sagar Kudale
XII
State Level Conference on ‘Microbology in 21st Century’
Finance and Sponsorship Committee
Advisor:
Prof. S. S. Thengdi
Members:
Mrs. A. A. Devale
Ms. S. S. Bhuyan
Ms. M. J. Amrutkar
Mrs. S. Puranik
Mr. D. Shah
Mr. A. Kakad
Certificate Preparation Committee
Advisor:
Prof. R. N. Ingole
Members:
Mrs. A. A. Devale
Ms. S. P. Bashetti
Mr. R. Dhabade
Mrs. R. Thombare
Hall Arrangement Committee
Advisor:
Prof. S. S. Deshmukh
Members:
Dr. (Mrs.) S. S. Mujumdar
Mrs. Ranjana Dhotre
Ms. S. S. Bhuyan
Mr. Tushar Bharekar
Ms. M. J. Amrutkar
Ms. S. P. Pardeshi
Mrs. A. Sardesai
Ms. A. V. Jagdale
Mr. A. A. Katkar
Mr. V. M. Daundkar
Mrs. J. J. Dunde
Mr. R. Shawalkar
Mr. Prasad Vanage
Mr. Rajendra Khapre
Mr. Sameer Dhavade
XIII
State Level Conference on ‘Microbology in 21st Century’
Hospitality and Food Arrangement Committee
Advisor:
Prof. Y. Thipse
Members:
Mrs. A. A. Devale
Ms. M. J. Amrutkar
Mrs. A. Sardesai
Mr. A. Kakad
Mrs. R. S. Sawant
Ms. S. P. Bashetti
Mr. P. Sabale
Publicity Committee
Advisor:
Dr. A. K. Pande
Members:
Dr. (Mrs.) S. S. Mujumdar
Ms. S. P. Pardeshi
Ms. S. P. Bashetti
Session In‐Charge
Advisor:
Dr. (Mrs.) Shilpa S. Mujumdar
Members:
Ms. S. P. Pardeshi
XIV
State Level Conference on ‘Microbology in 21st Century’
Volunteers
Aniket Hambir
Anuradha Kulkarni
Baviskar Ravindra Dhondu
Bedare Monika Kundalik
Bhong Yogita D.
Bhoodatt Mrinmai R.
Bidkar Monali A
Chaudhari Saurabh Renukadas
Chhajed Khushbu V.
Chothe Nital Syam
Churi Ruta K.
Daithankar Bhagyashree D.
Dasarwar Shriniwas Vijaykumar
Deore Poonam Vasant
Deshmukh Sujit M.
Deshpande Manisha M
Dhamal Hrishikesh Dilip
Gaikwad Sarika
Gaikwad Sunny Tanaji
Gavhane Supriya S.
Ghole Tushar S.
Inamdar Bhagyashree Anant
Jade Rupali S.
Jadhav Amar Dnyaneshwar
Jadhav Kanchan Janardan
Jadhav Shweta M.
Jere Saee S.
Kadam Monika A.
Kale Trupti P.
Kalel Preeti V.
Kanchan Jadhav
Kasyap Poonam R.
Kamelia Behroozi
Tejaswita Pawar
Yadav Priya
Shelar Prachi
Reshma Yeole
XV
Khude Rasika R.
Konde Pallavi
Lande lalit Satyawan
Londhe Madhuri Hanumant
Malla Shikha
Mandal Mamta Rajendra
Mane Deepali Sahebrao
Mane Priyanka Vasant
Mehendaley Saili Vishwas
Nadivkar Pradnya Nandkumar
Naina Gaikwad
Mazhar Tahaseen
Pallavi Konde
Patil Datta V.
Patil Pratibha Abhimanyu
Pawar Gauri Manohar
Picha Pankaj Sudhirkumar
Punde Priya S.
Raut Tushar C.
Ravindra Baviskar
Rohani Neha Giridhar
Sakhare Reshma Ramakant
Sanam Vynkatesh Madhukar
Sen Divya Deepakkumar
Sheetal Joshi
Shelar Akshay B.
Shetty Rashmi Deepak
Shrinivas Dasarwar
Singh Ajay Pal
Sujata Shinde
Telore Snehal Vilas
Thorave Varsha J.
Jagtap Bhagyashree
Phulphagar Akshata
Manisha Shelar
Madhuri Wadkar
State Level Conference on ‘Microbology in 21st Century’
Contents – Oral Presentations
Abstracts
OP1
OP2
OP3
OP4
OP5
OP6
OP7
OP8
OP9
OP10
OP11
OP12
OP13
Title and Authors
Candida glabrata Infection of The Nasal Septum – An Unusual Case
Report
Roma A. Chougale
Prevalence of Methicillin Resistant Staphylococcus aureus in D. Y.
Patil Hospital And Research Centre
Reena Dighe and Saral Ghosh
Bacteriological Quality of Water, Sanitation Interventions And
Health With Special Reference To Diarrhoea.
Anil M. Garode
Screening of fungal isolates for lignolytic enzyme, manganese
peroxides
Madhuri A. Udata, Joshi A. J., Mahendra K. Ranjekar
Biotransformation of Textile Azo Dyes by Aerobic Bacteria.
Ravi V. Kale and Prakash R. Thorat
A cost-effective protocol for cultivation, microbial and biochemical
analysis and high yielding nutritious variety of mushrooms.
Jogi. M. M., Supriya V. Suryawanshi, Bhagyashree V. More, Nilesh P.
Anandwani, Jayesh A. Patil
Page
57
Biosurfactant production by a marine strain of Pseudomonas.
Vijendra A. Kavatalkar, Smita S. Bhuyan, Peehu S. Pardeshi, Balu A.
Chopade
Antiplasmid activity of herbal extracts on Methicillin Resistant
Staphylococcus aureus
Avinash D. Bholay, Anita N. Katkade, Kaveri S. Palekar
Study of extended spectrum beta lactamase (ESBL) producing Gram
negative bacilli in family Enterobacteriaceae.
Vishwashanti. S. Vatkar, P.G.Shadija, S.J.Ghosh
Microbial Bioaugmentation for treatment of industrial effluents in
Common Effluent Treatment Plant (CETP)
Snehal B. Bari, Nilesh A. Sonune, Amit R. Sinnarkar, Seema S. Sarnaik,
G. K. Wagh, Pradnya P. Kanekar
Isolation of Rhizobium and Optimization of Growth Conditions for
Development of Biofertilizer for Trigonella foenumgraecum
(Fenugreek)
Aasawari S. Pawar and Rajendra Choure
Synthesis and characterization of ZnO nanoparticles and their
application on textiles as antimicrobial agents and UV absorbers.
Rutuja.R.Phatate, Rebecca.S.Thombre and Sonal.P.Bathija
Comparative study on the production of Prodigiosin by Serratia
marcescens using various crude fatty acid sources and its
applications
Pankaj .S. Picha, Sheetal .P. Pardeshi
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State Level Conference on ‘Microbology in 21st Century’
Contents – Poster Presentations
Abstracts
PP1
PP2
PP3
PP4
PP5
PP6
PP7
PP8
PP9
PP10
PP11
PP12
PP13
PP14
Title and Authors
Isolation and Characterization of Listeria species from
environmental and clinical sample
Abhay Raorane & Barbuddhe S.B.
Antimicrobial activity of Vinca rosea (Catharanthus roseus
L.) and Clematis gouriana against pathogenic bacteria
Akshay Borkar, Manoj Bhosale, Shirish Shah,
Salokhe S.G., Sonawane H.V
Isolation and Screening of plant growth promoting bacteria
from rhizosphere
Alka Jagdale,Shradha Bashetti, Smita Bhuyan,
Shilpa Mujumdar, Anushka Devale
Isolation of Poly-β-Hydroxyalkanoate Producing Bacteria
from waste water and optimization of its production
parameters
Amar D. Jadhao, Hrishikesh D. Dhamal, Vivek N. Bobade
Characterization of contaminants of cosmetics and their
antibacterial sensitivity against synthetic and natural
preservatives.
Ayushi.A.Chaurasiya And R.B.Vaidya
Study of quorum sensing and quorum quenching in Vibrio
fischeri and application in aquaculture
Archana J. Marry & Jyoti Mantri
Page
02
Biodegradation of Triaminotrinitrobenzene (TATB)
Abhijit More, Sayali Mahajani, Seema Sarnaik & Pradnya
Kanekar
Production of amylase by bacteria isolated from soil sample
Bedare Monika & Madhuri Amrutkar
Characterization of thermo-tolerant and acid /alkali tolerant
β glucosidase from bacterial isolate
Bhalkikar S.B. , Diwan V.A., Ranjekar M.K
Detoxication of Azo Dyes by microbial pure cultures as well
as microbial consortia.
Bholay A.D., Maid Sneha A., Borse Priti k., Rakte Snehal B
Probiotic properties of Lactobacillus isolates from milk of
domestic animals and Commercial available probiotic
preparations against enteric bacterial pathogens
Bhutada SA & Tambekar DH
Isolation , purification & application of lipase enzyme.
Bhadale S. V, Bhalerao M.N, Patil N.N, Ghule P.V & Kulkarni
Antibiotic Production from Bacillus spp. Active against
Staphylococcus aureus
Chavan K.J, Patil S.D, Ranjekar M.K.
Antimicrobial activity of mangroves against multidrugresistant (MDR) pathogens.
Bholay.A.D, Kurup Sunila .B., Vidhate Pushpa.S.,
Rahatekar Rohini .R.
09
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State Level Conference on ‘Microbology in 21st Century’
PP15
Biosorption of hexavalent chromium from aqueous solutions
using dead fungal biomass of P. chrysogenum
Deep.V.Barot & Varsha.Vaidya
Study of antibiotic resistance patterns in Campylobacter
spp. isolated from Pune, Maharashtra
De Souza NA, Kamble A, Parkar SD, Kapadnis BP
Antimicrobial Activity of new Sulphanylamide Analogues
against Commonly occurring Multidrug Resistant Urinary
Tract Pathogens
Gauri Devasthale, Alok Vyas, Divya Nair & Jhilmil Ghoshal
Isolation,
identification
and
characterization
of
bioluminescent bacteria from fish sample and its use as
water pollution indicator
Gauri Pawar, Deepali Mane, Vyankatesh M. Sanam,
Shilpa Mujumdar & Smita Bhuyan
Assessment of genetic diversity among mango varieties by
RAPD markers
Hrishikesh Joshi, A. R. Desai
Development of microbial biostimulants to enhance fertility
of saline soils for sustainable agriculture
Indulkar V.S. Siddiqui A.G., Ranjekar M.K
Assessment of Water Purification Potential of Moringa
Oleifera Coagulant Protein (MOCP)
Jain Reena R & Kadam Shradhha D
Isolation of Bacteriophage against Campylobacter from
Poultry in Pune
Kamble A, De Souza N, Parkar S, Kapadnis BP
Isolation and Identification of Pathogens of Seafood Penaeus
sp.
Ketki Nalawade & Rajendra Choure
To study anti-bacterial activity of various medicinal plants
against mixed dental flora
Khadpekar A & Thombre R.
Screening & Production of Laccase from Fungi
Kawade S.S, Diwan V.A., Ranjekar M.K
Studies on bioemulsifier production from Provdentia stuartii
isolated from garden soil.
Lalit Lande, Smita Bhuyan & Shilpa Mujumdar
Comparative study of bioinoculants prepared from
rhizosphere soil and non-rhizosphere soil.
M. S. Vrushali ,N.Rutika
18
PP28
Isolation of siderophore producing organism
rhizosphere soil of Adathoda vasica
Madhuri Londhe, Smita Bhuyan,Shilpa Mujumdar
33
PP29
Comparative Analysis of Faecal Microbiota from Normal
and Obese Wistar rats.
Mahesh R Ghule,Rajesh M Chomal ,Deepak R Patil &
Yogesh S Shouche
PP16
PP17
PP18
PP19
PP20
PP21
PP22
PP23
PP24
PP25
PP26
PP27
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State Level Conference on ‘Microbology in 21st Century’
PP30
Production of bacterial cellulose by Acetobacter xylinum
(NCIM 2526) using fruit waste and checking for its
application
Manasi Karmarkar, Poorva Dabir & Meghana Kukarni
Aureobasidium Pullulans Grown On Various Feedstocks
For Comparative Studies
Meenakshi Dhar, Nasim Kherad, Vinay Rale
Antimicrobial effect of bombax ceiba
Monika More, & Gauri Jadhav
Production and purification of extracellular RNases from
Streptomyces venezualae (NCIM 2215).
Mokshada Godbole, Neelima Kulkarni & S. S. Deshmukh
Isolation screening for penicillin- v acylase producing
organism optimization of culture conditions, effect of
media constituents on production of enzyme from B.
cereus
Neha Naik, Avinash Sunder, Laxmi Nair,
Vaidehi Dande & A.V. Pundle
Characterization of plasmid encoded resistance to β-lactam
antibiotics of Acinetobacter baumannii AIIMS7, a clinical
isolate
Neha S. Vora, Shradha B. Nadhe, Riddhi J. Shah &
Karishma R. Pardesi
35
Optimization of growth parameters for ethanol production
by yeasts
Neelima Kulkarni, Shreyas Kumbhare, Pandurang Lahare,
Hussain Khokhawala
Comparative In-vitro Antimicrobial activity of the extract of
Gloriosa superba against laboratory and antibiotic resistant
clinical isolates of common pathogens
Nikita L. Lad and Rajendra G. Choure
41
PP38
Extracellular RNase production and purification
from Streptomyces sp. (NCIM 2081)
Niruta Y. Killedar, Anupama Sonawane and Sumedh S.
Deshmukh
43
PP39
Isolation and characterization of oxalate degrading
organism
P.S, Patil , Patil Sham A , Bedse,Tanuja D, Jadhav Sandip R .
Oligotyping of antibiotic resistance genes from
clinical isolates of Acinetobacter spp.
Poojari Pritika, Ratnakaran Neena, Mehta Khyati,
Shirode Abhinav, Pardesi Karishma
Optimization of Amylase, production by fungi isolated from
rhizosphere soil.
Pradnya N. Nadivkar & Sheetal. P. Pardeshi
Reduction of heavy metals from domestic sewage by using
Microorganisms
Prajakta Bhagwat, Priya Bendre and Vrushali Patil.
44
PP31
PP32
PP33
PP34
PP35
PP36
PP37
PP40
PP41
PP42
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State Level Conference on ‘Microbology in 21st Century’
PP43
PP44
PP45
PP46
PP47
PP48
PP49
PP50
PP51
PP52
PP53
PP54
PP55
PP56
PP57
PP58
Isolation and characterization of indole acetic acid (iaa)
producing organism from rhizosphere of gram (Cicer
arientinum).
Priyanka Mane, Smita Bhuyan & Dr. Shilpa Mujumdar
Wound Healing Properties of honey.
Purnima Ranawat & Jyoti Mantri
Optimization and characterization of lipase production by a
selected Bacillus sp. and its application
Rahul Brid and Rajendra Choure
Laboratory Study on Bioremediation of Diesel Oil
Contaminated Soil from a Garage
Rakhi Tari & Shilpa Sabnis
Production of giberrelic acid by soil fungi
Ravindra Baviskar, Sunny Gaikwad, Shilpa Mujumdar & Smita
Bhuyan
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Production of Xanthan gum by Xanthomonas campestris
using Industrial and kitchen waste as a production media
Repe A.B., Dhutekar S.S,Ranjekar M.K.
Biological synthesis of silver nanoparticles and evaluation of
their antibacterial activity
Sabah Shaikh & Shilpa Sabnis
Study of ocular microflora, antimicrobial and antiadhesive
activity of MPS solutions on ocular isolates.
Sagar Vandra & Shilpa Sabnis
Identification and extraction of tobacco mosaic virus (DNA)
using molecular techniques
Samriddhi R. Sharma & Aparna Das
Phylogenetic analysis of insulin (protein) using
bioinformatisc tools
Sandeep Gajbhiye, Mr. Sunakar
Microbial degradation of Diaminodinitroethene,
a high energy nitroexplosive
Sayali Mahajani,Abhijit More, Seema Sarnaik & Pradnya
Kanekar
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Determination Of Resistance Of Listeria monocytogens
Towards Sanitizers And Antibiotics In Presence Of Tween
80
Shahina Shaikh, Abhay Raorane & Deepali Giremar
Antibacterial activity of crude extracts of spices against food
borne pathogenic microorganisms.
Shikha Malla; Shilpa Mujumdar; Madhuri Amrutkar
Evaluation of antibacterial activity of pigments produced by
Pseudomonas aeruginosa
Shilpi.R.Chand & R.B.Vaidya
Biodegradation
of
Hexavalent
Chromium
Using
Microorganisms
Singh Viveka , Srivastava Shreya and Gore Suneeti
Study of Synthesis of Silver nanoparticles.
Sourabh Mehta, Rebecca Thombre, Shubhangi Puranik.
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PP59
PP60
PP61
PP62
PP63
PP64
PP65
PP66
PP67
PP68
PP69
PP70
PP71
PP72
Decolorization of textile dye direct lemon yellow
by Burkholderia cepacia
Sunil. S. Tiwari & Sangeeta. K. Jangam
Phytochemical and antibacterial screening of solvent
extracts of leaves of Bauhinia racemosa Lam.
(Caesalpiniaceae)
against enteric bacterial pathogens
S.B.Dahikar, S. A. Bhutada, D.H.Tambekar, and S. B. Kasture
Viral Diagnosis by using RT – PCR: A case study on Banana
Chlorosis Disease
S.O. Gupta & B.A. Aglave
Biopulp - an eco-friendly approach
S. M. Kulkarni, Navale S. K.,Sulkshane H. C.,Sharma L. P.,
Khandelwal S.R., Shirke M. D., Kokani N. F., Bhavsar S. P., &.
Joshi S. A.
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Plasmid Profiling and Antibacterial Activity Of Garlic
Extract And Clove Oil On Clinical Isolates Of Pseudomonas
aeruginosa
Tejlaxmi Inamdar, Milky Sahukar, Kavita Parekh.
Screening of Lipases as potential virulence factor in
Acinetobacter species isolated from different hospitals in
India.
Urvi Doshi & Karishma Pardesi
In Vitro Studies of Antimicrobial Properties of Extracts from
Unani Medicinal Plants
Vaishali Prabhune, Namra Ghansar, Supriya Bhopale
Lignin degrading peroxidase in biobleaching of paper-pulp
mill and textile dye-based effluents
V. A. Tile, Borkhataria B. V, Bhalerao V. B, Aher N. S.
Bacterial synthesis of silver nanoparticles and its
applications
V.A.Tile., S.P.Pawar., W.R.Sayyed., A.V.Bhosale
Accumulation of polyhydroxyalkanoate(PHA) from styrene
by Pseudomonas spp and effect of N2 concentraction on PHA
accumulation.
Vidya N. Udawant, Smita A. Daule & D. D. Giramkar
Utilization of molasses for biosurfactant production
by Streptomyces S2
Vynkatesh. M. Sanam, Deepali S. Mane., Gauri M. Pawar,
Shilpa. S. Mujumdar & Smita. S. Bhuyan
Isolation, Characterization and Application of Phenol
Degraders
Uzma T. Momin and Varsha K. Vaidya
Antibacterial Activity of Medicinal Plants against
Gastrointestinal Pathogens
Pratibha A Patil , Shilpa S Mujumdar, Madhuri Amrutkar.
Utilization of petroleum hydrocarbons by bacteria isolated
from soil & water contaminated with engine oil
Dalvi, C. S., Satbhai, M. A., Bhutada, S. A.
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PP74
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Biodegradation of commercially used insecticides by soil
isolates
B. A. Karode, N. B. Choudhari, S. S. Patil, P.T. Patil & A. H.
Jobanputra
Biodegradation of diesel oil by soil isolates
V. R. Kothari & A. H. Jobanputra
Raw wastes as alternative media for Trichoderma: Towards
sustainable development
Rushikesh Sankpal, Sunil Deshmukh & Vinay Rale
Characterization and biodiversity of Pashan lake water
Rahul Nimbalkar & Varsha Kulkarni
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State Level Conference on ‘Microbology in 21st Century’
Extended Abstract
Prokaryotes and Eukaryotes: Do They Talk to Each Other?
Prof. S. Mohan Karuppayil
School of Life Sciences, SRTM University, Nanded. 431606
A paradigm shift is happening in our understanding of the world of
unicellular eukaryotes and prokaryotes. The microorganisms, like bacteria and yeasts
are often considered as non cooperative individualistic organisms. In fact, they are
very social organisms and in nature they tend to live as communities called as
biofilms and often behave like multicellular organisms. Biofilms are multicellular
assemblages of cells encased in extracellular matrixes. They are interactive and use
small diffusible molecules for communication, through a process referred as quorum
sensing. They release small diffusible molecules which parallel with cell density and
bind to sensors when they reach a critical concentration. The bound proteins activate
the transcription of various genes resulting in behavioral changes. Hundreds of genes
are regulated by the quorum sensing or chemical signaling molecules. The genes
regulated include those which govern luminance, pathogencity, virulence factors,
symbiosis/predation to cite a few. The chemical language used by microorganisms
may be species/ genera specific or general. In short, microorganisms can detect the
presence and density of not only their own species, but also members of other genera
or even kingdom. Most of the time, the biofilms are not homogenous assemblages but
polymicrobial. For example, in cystic fibrosis patients Pseudomonas aeruginosaCandida albicans biofilms are common. Various studies shown that bacteria and
fungi are not silent partners in biofilms but are engaged in meaningful dialogues
resulting in changes in gene expression and observable behavioral changes.
Eukaryotic hosts may recognize the quorum sensing molecules produced by the
pathogens and may adapt strategies to handle it. Quorum sensing molecules of the
pathogens are shown to elicit responses in the host often in favor of them or vice
versa. The prokaryote - eukaryote dialogue is not only of importance to pathogenecity
or virulence but may also influence secondary metabolite production, symbiosis
and/or predation. Understanding the prokaryote-eukaryote dialogue may open up new
opportunities for drug development and also reveal the marvelous biology behind the
interactions.
XXIII
State Level Conference on ‘Microbology in 21st Century’
Extended Abstract
Decolorization and Degradation of Textile Azo Dyes by
Microorganisms
Dr. P. R. Thorat
Prakash_micro1@yahoo.co.in
Department of Microbiology and Research Center, Shri. Shivaji Mahavidyalaya,
Barshi – 413411, Dist. Solapur, Maharashtra
Water is the most precious divine natural resource that exists on our planet.
Environment and life are in very close relationship on earth. Humans must protect
these natural resources to lead life happily, but rapid industrial development and
population explosion has made the problem of environmental pollution more and
more acute. In India maximum population inhabits in villages and slum areas, where
there is no safe drinking water supply. Dyes and pigments are used in various
industrial processes like, textile, pharmaceutical, cosmetics, food, printing, etc. The
textile industry is the largest, indispensable and backbone industries of some underdeveloped and developing countries in the world. The water required for textile
industry is very large. Azo dyes that are used in the textile industries are xenobiotic
compounds which contain (-N=N-) linkage and which are mutagenic as well as toxic
to living organisms and environment also. Textile industry is confronted with the
problem of color removal and effluent salt content reduction.
The most pressing environmental problem facing the textile industry is the
dye containing waste-water. The dye containing effluent represents highly
problematic wastewaters not only because of high COD, BOD suspended solids, toxic
compounds, but also because of color (dyes) which make them easily recognized and
poses esthetic problems. When dyes are released in the environment, they form toxic
compounds which harm the living creatures. Also the dyes color the water reservoirs
which reduce the penetration of sunlight in turn leading to eutrophication. Presently
there is no economical and efficient means or process to achieve the reduction of
these two parameters. Now-a-days dye wastewater is treated by physical and chemical
procedures which have many shortcomings. These physico-chemical methods
available for the treatment of these synthetic dye-stuff, like flocculation, sorption,
electrochemical and oxidative degradation. These physico-chemical methods
available for the treatment of these synthetic dye-stuff, like flocculation, sorption,
electrochemical and oxidative degradation which have limitations like cost
effectiveness sludge or residue formation which is difficult to dispose off and also
limited in their applications.
Many studies have been focused on the microorganisms that are able to
degrade the dyes, suggesting the bioremediation as an environment friendly and cost
competitive alternative for dyed wastewater treatment. Decolorization of the synthetic
XXIV
State Level Conference on ‘Microbology in 21st Century’
dye using different microorganisms appear to be effective, environment friendly, less
expensive and with reproducible biotransformation data. Dye-stuffs are degraded by
range of microorganisms in nature but studies are concentrated on bacterial and fungal
decolorization of the dye.
Release of azo dye in the environment is of great concern due to, colour,
toxicity, mutagenicity and recalcitrant nature of the dye, considerable attention has
been given in determining the ability of microorganism in decolorization and
degradation of the azo dyes. In the present study, acclimatized microorganisms
isolated from natural sources were used for the study of decolorization and
degradation of the dyes viz. Acid Blue - 113, Acid Red - 1, Reactive Orange - 16,
Direct Yellow - 50, Yellow - 4G, Orange - 2R, Methyl Red and Disperse Brown 3REL. In all total 63 microorganisms were isolated, these isolates showed good
decolorization pattern with average of 97.95% in nutrient broth condition. In Half
Strength Nutrient Broth condition, the isolates showed 86.33% decolorization of the
dyes. The cell free extract of the isolates showed 89.07% decolorization of the dyes.
The percent COD reduction of the dyes was determined, the isolates showed upto
88.00% reduction in COD of the dyes. The degradation of the dyes was determined by
GC-MS analysis technique. The microorganisms were identified by 16s rRNA
analysis technique.
Key Words
Decolorization, Degradation, Aerobic, Bacteria, Azo Dyes, COD reduction
XXV
State Level Conference on ‘Microbology in 21st Century’
Extended Abstract
Microbial Solubilization of Metals from Waste Printed Circuit
Boards
A. M. Deshmukh
amdeshmukh1@rediffmail.com
Department of Microbiology, B. A. M. U. Subcampus, Osmanabad 413501,
Maharashtra
With the rapid development of technology, the problem of older electronic
equipments is increasing. The main reason behind such increase is low rate of
recycling which, in turn, is due to the complex nature of such waste. Traditional ways
for treating such waste are not economical and they contribute to pollution. The
promising answer for the problem is bioleaching.
Studies were carried out to solubilize heavy metals from electronic waste.
For this purpose, the consortium was obtained using a simple way, similar to that of
‘top down’ approach. Printed circuit boards (PCBs) were used as representative
samples of e-waste. Various concentrations of PCB powder were used (1-5%) for
bioleaching and their effects on metal solubilization, changes in pH and concentration
of ferrous iron were accessed. Maximum level of metal solubilization was found to be
96.93% Cu, 93.33 % zinc for 10 g/litre of PCB powder and 10.26 % Ni for 30 g/litre
of PCB powder. In case of lead, only 0.58% solubilization was achieved when 20
g/litre of PCB powder was used. The precipitate formed during bioleaching was
analyzed by SEM EDAX which shows the presence of Tin (59.96%), Cu (23.97%),
Pb (9.30%) and Fe(5.92%). The causes of copper precipitation were discussed.
Key Words
Microbial consortium, Printed circuit boards, Bioleaching
XXVI
State Level Conference on ‘Microbology in 21st Century’
Extended Abstract
Recent Trends in Microbial Control of Phytopathogens
S. B. Chincholkar, B. L. Chaudhari, P. D. Sarode and M. R. Rane
sudhirchincholkar@gmail.com
School of Life Sciences, North Maharashtra University, Jalgaon 425001
Progress in molecular biology and analytical methods has helped in
understanding of biological control of soil borne phytopathogens. There exists a great
diversity among pathogenic fungi and bacteria. Therefore, control of such pathogenic
micro-organisms is a great challenge. Plant Growth Promoting Rhizobacteria (PGPR)
and Bio-control Agents (BCA) having multiple activities is a proper antidote against
such phytopathogens. Earlier practices like – fighting of phytopathogens with soil
fumigants and using agricultural practices like – crop rotation, tillage is either harmful
or inadequate. The recent progress in technology focuses on knowledge on
mechanism of competition of food, space, iron, parasitism, induction of systemic
resistance and antibiosis. Recent approaches eg. identification of plant genes which
improve plant interaction with disease suppressive bacteria, identification of genetic
factors of biocontrol strain which alter/diminish biocontrol feature eg. phase variation,
identification of factors produced by phytopathogens which alter biocontrol
mechanisms eg. fusaric acid reduce anti-fungal metabolite production and also
interfere quorum sensing/cell signaling, manipulation of soil mineral properties to
optimize biological control would strengthen the efforts of agriculturists in time and
space. Out of available novel biocontrol agents, our laboratory has concentrated on
phenazines. Phenazines are heterocyclic, nitrogen containing, brightly colored
secondary metabolites produced by a variety of bacteria. They exhibit anti-fungal as
well as anti-bacterial activity. We concentrated on Pseudomonas aeruginosa 4365
which secretes phenazine-1-carboxylic acid (PCA) and pyocyanin. Standard protocol
for laboratory scale production of PCA and pyocyanin was developed. Both
compounds were purified to obtain crystals. This purified material was characterized
with the help of UV-Visible Spectroscopy, IR, NMR and HPLC. Anti-fungal activity
was studied in vitro. Pyocyanin, though a weak antifungal showed pH dependent
inhibition of Scelrotium rolfsii. PCA is comparatively strong antifungal compound
and inhibited Fusarium oxysporum, Sclerotium rolfsii and Colletotrichum falcatum at
very low concentrations. Scaled up production of PCA at 12 L and 125 L was carried
out. An attempt was made to evaluate comparative activity of PCA as a foliar
fungicide through field trial on chilli which demonstrated that PCA has, if not better,
comparable activity with various commercial chemical fungicides. Process details and
other technical information will be discussed in conference.
XXVII
Extended Abstract
Abstract of the Presentation
Dr. D.V. Gokhale
NCIM Resource Center, National Chemical Laboratory, Pune 411008, India
Microorganisms, their cells or their replicable parts are the tools for
biotechnology. These microbes are diverse and show numerous metabolic activities
leading to products of immense industrial importance. The vast number of
microorganisms has been isolated, identified conserved and utilized for benefit of
mankind. However, 99% of the environmental microbes cannot be cultured under
laboratory conditions. Thus the majority of the microbes thus remain hidden which need
to be explored, identified and also conserved. These untapped microbial sources can be
exploited using metagenomic approach to obtain novel microbial products. All these
microbial strains need to be preserved in pure form without losing their genetic as well
as phenotypic identity. Microbial Resource center (MRCs) act as custodians of such
microbial diversity and play an important role in the storage and supply of authentic
reference cultures for research and development. Microbial culture collections are
established in many countries having variety of purposes. There are very small culture
collections (200 microbial strains) and also large cultures collection possessing more
than 50,000 microbial strains.
NCIM Resource Center is a pioneer microbial culture collection facility in
India, which offers services to educational / research institutes and industries. NCIM is a
unique resource dedicated to the isolation, collection, preservation and distribution of
authentic cultures of industrially important microorganisms. NCIM holds about 3500
microbial strains of bacteria (1500), yeast (550), fungi (1300) and algae (15). The main
objective of the facility is to supply authentic cultures to research institutes and
industries. The catalogue of microbial strains can be accessed through http://www.nclindia.org/ncim. Ours is small culture collection compared to ATCC and NCIMB.
However, I will discuss some of the achievements emerging out of screening our own
microbial sources.
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP111
Isolation oof Rhizobium
m and Optimiization of Growth Conditiions for
Develoopment of Biiofertilizer foor Trigonella ffoenumgraeccum
(Fenugrreek)
Aasawa
ari Pawar and
d Rajendra Ch
houre
Depaartment of Micro
obiology, The Insstitute of Sciencee, Mumbai 4000332
Trigoonella foenumgrraecum (fenugreeek) is known foor its rich dietarry proteins,
medicinal prooperties which arre supported by symbiotic nitroggen fixation by Rhizobium
in its root noddules. These prop
perties get comppromised by imbbalanced nitrogenn supply as
chemical ferttilizers. Biofertiilizers are conssidered to mainntain the nutriitional and
organoleptic qqualities of the leafy vegetable. The present stuudy describes thee screening
of a Rhizobiium strain isolaated from root nodules of feenugreek, optim
mization of
cultivation coonditions and application of Rhizobium biiofertilizer. Isollates were
characterized by analysis of growth rate, aciid production,400oC tolerance, toolerance of
2% NaCl, annd symbiotic peerformance. Thhe Rhizobium issolates were found to be
temperature annd pH sensitive,, (optimum values of 25°C and 88.0, respectivelyy). Medium
composition for laboratory scale growth was optimizedd using Plackeett-Burman
These isolates grown
g
on the optimized
o
mediuum were testedd for their
approach. T
differential efffect on germinaation of seeds and
a plant growtth promotion off fenugreek
seedlings of ccultivars S1 and
d S2, in vitro inn sterile Petri-plaates as well as in vivo in
different soils, SL1 and SL2. Germination
G
rate was higher in booth the seedlingss S1 and S2,
treated with thhe Rhizobium cultures, than conntrols which weere untreated. Inn pot assay,
pots seeded w
with Rhizobium,, seeds showed enhanced plant growth than unninoculated
controls, thouggh there was con
nsiderable difference in growth ppattern of test plaants.
Published on 25tth February 2011
Page 1
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
P
PP01
Isolaation and Ch
haracterizatioon of Listeria Species from
m
Environ
nmental and Clinical Sam
mple
Abhay Raorane¹, Ba
arbuddhe S.B
B.²
1
@gmail.com
abhay.micro@
Deparrtment of Microb
biology New Artss Commerce andd Science Collegge,
Ahmednaggar,
2
ICAR, Ela, Oldd Goa, Goa
Listeeria spp. are ubiiquitous in natuure. Out of 8 sppp. 2 are pathoogenic to
humans and aanimals. 14 env
vironmental and 133 clinical sam
mples were screeened for
presence of L
Listeria spp. Ou
ut of 14 environnmetal samples, 2 (14.28%) andd of 133
clinical samples, 25 (18.79 %) were positiive for Listeria spp. Biochem
mical and
genetical studdies confirmed that 11(7.48%) were pathogeenic strains of Listeria
monocytogenees. CAMP and ALOA tests were
w
performed to confirm the isolates.
Further serotyyping study of th
hese 11 pathogennic strain revealeed that 5 isolatess were of
4b serotype aand 6 were 1/2b serotype. Otherr species detecteed were L. ivannovii and
other Listeria spp. No environmental samples contained pathhogenic spp. of Listeria.
ward the antibbiotics viz. Am
mpicillin, Doxyycycline,
Isolates weree sensitive tow
Ciprofloxacin, Vancomycin and shows intermediate rresistances tow
ward the
G
Chlorampheniicol, Penicillin, Gentamycin.
Key woords
Listeria spp, ALOA
A
test, CAM
MP test, Serotypping, AST.
Published on 25tth February 2011
Page 2
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP002
Antimicrobial Activity of Vinca Rossea (Catharan
nthus Roseuss L.) and
Clematis Gou
uriana againsst Pathogenicc Bacteria
Aksha
ay Borkar, Ma
anoj Bhosale,, Shirish Shah
h, Salokhe S.G
G.1,
2
Sonawane H.V.
AkshayBorkar1@gmail.com
Deparm
ment of Zoology, Annasaheb Maggar College, Haddapsar, Pune- 411028.
2
Dept. of Microbiology, Annasaheb Maggar College, Haddapsar, Pune-4111028.
1
a (Catharanthuss roseus L.) annd Clematis gouuriana has
Vinca rosea
historicaally been used to
o treat a wide assortment of diseeases. Catharantthus roseus
L. has wound healing activity in diaabetic patients. Clematis gouriiana is an
nt of western ghats
g
and India, used in the treeatment of
endemicc medicinal plan
dermatoppathy, blood disseases, leprosy, wound healing, viral fever, heaadache and
cardiac disorders. Ethan
nol extract and water extract of Catharanthus roseus L.,
e
and
methanool and water extrract of Clematis gouriana was sttudied. Ethanol extract
water exxtract of Catha
aranthus roseuss L. of 50mg/m
ml, 100mg/ml, 150mg/ml
concentrration was studieed and methanoll and water extraact of Clematis gouriana
g
of
50mg/m
ml, 100mg/ml, 150mg/ml conceentration were sstudied. All plaant extracts
were cheecked for Minim
mum Inhibitory Concentration aand Minimum Bactericidal
B
Concenttration. Then th
he extracts werre evaluated too find out phyytochemical
compounnds by Thin layeer Chromatograpphy.
Published on 25tth February 2011
Page 3
Progressivve Education society’s
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
Depaartment of Microb
biology
State Level Conference on
Miicrobiology in 211st Century
25th – 26th February
F
2011
Absttract Book
PP003
Isolation and Screenin
ng of Plant Growth
G
Promooting Bacteriia from
Rhizosp
phere
Alka Jag
gdale, Sharadha Basetti, Sm
mita Bhuyan,, Shilpa Mujumdar,
Anushka Devale
Department oof Microbiology,, Modern collegee of Art, Sciencee and Commerce,, Pune 05.
Plantt growth promotting bacteria (PG
GPB) are soil annd rhizosphere baacteria that
can benefit pplant growth. Given
G
the negaative environmeental impact off chemical
fertilizers andd there increassing cost, the use of PGPB as natural fertilizers is
advantageous for the devellopment of susstainable agricuulture. 20 strainn showing
phosphate sollubilization activ
vity and 6 strainns showing nitroogen fixation acttivity were
isolated from farm soil located
d near Saswad. The
T isolate show
wing both the acttivities was
selected for thhe further studies and identifieed as Staphylocooccus hominis by
b API kit.
Application oof Staphylococccus hominis cuulture with lignnite showed plaant growth
promoting acttivity. Staphyloco
occus hominis also showed posiitive Siderophoree and Indol
Acetic Acid prroduction.
Key Woords
Phosphaate solubilization
n, Nitrogen fixatiion, plant growthh promoting baccteria.
Published on 25tth February 2011
Page 4
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP004
Isolation
n of Poly-β-H
Hydroxyalkan
noate Produccing Bacteria from
wastee water and optimization of
o its producttion parametters
Ama
ar D. Jadhao, Hrishikesh D.
D Dhamal, Viv
vek N. Bobad
de
@gmail.com
viveknbobade@
Departmeent of Microbiollogy, Modern Coollege of Arts, Sccience and Comm
merce,
Shivajinagarr, Pune 5.
Polyhhydroxyalkanoattes are considered good substituutes for petroleuum derived
synthetic plasttics because of their
t
similar phyysical and chemical properties and
a can get
degraded com
mpletely to CO2 and water undder natural envirronment by the enzymatic
activities of microorganismss. Poly-β-hydroxxyalkanoates (P
PHA) are synthhesized by
numerous baccteria as an inttracellular carboon and energy storage compoound under
limited conceentrations of nuttrients with exccess carbon. Thee objective of the
t present
study is to isolate the bacteria from waste watter and that are ccapable of produucing poly t optimize
β- hydroxyalkkanoates and theeir characterization. The study also carried out to
the growth parrameters such ass pH, temperaturre, time, nutrientt concentrations etc. Waste
water containiing mostly sewaage sample from Siddheshwar G
Ghat, Mulla riverr, Pune was
collected. It iis screened for bacteria
b
produciing poly – β – hydroxyalkanoaate (PHA).
Detection of P
PHA production was done by Suudan black staininng and Spectropphotometry.
The media opptimization was carried out usinng modified E2 medium containning either
one of the vaarious carbon so
ources such as Glucose,
G
Fructoose, Maltose, Suucrose, and
either one of tthe Nitrogen sou
urces such as Am
mmonium chloridde, Ammonium sulfate and
ammonium Phhosphate and yeast extract. Otheer growth param
meters such as pH
H (5.0, 6.0,
7.0 and 8.0), ttemperature (amb
bient temperaturre, 28 oC, 30 oC, and 32oC) and time
t
(about
24hr, 48hr andd 72hr) were opttimized for produuction of PHA. The bacterial isoolates were
designated as Wi1 to Wi30 (W=Waste
(
water, i=isolate) werre found to prodduce PHA.
W Wi4, Wi8, and Wi13 were selected for evaluation of
Among these four cultures Wi2,
meters for maxim
mum PHA prodduction, since thhese are found to
t be most
growth param
promising PH
HA accumulatin
ng bacteria. Among the paraameters evaluatted in the
independent eexperiments 1g % glucose, 0.1 g % ammonium
m phosphate, tim
me of about
o
48hr, temperaature 32 C and pH
p 7.0 were founnd to be suitablee for maximum production
of PHA by W
Wi2, Wi4 and Wi8.
W
Wi13 alsoo shows maximuum production of PHA at
above concenttrations except pH
p for maximum
m production of P
PHA is 8.0
Key Words
Poly–β–hydrox
xyalkanoate, PHA
A, Optimization of growth param
meters
Published on 25tth February 2011
Page 5
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP003
Bacterioological Quality of Waterr, Sanitation IInterventionss and
Health with
h Special Refference to Diarrhoea.
A. M. Ga
arode
anilgarode@yymail.com
Department of Microbiologyy, Shri. Shivaji Science
S
and Arts College, Chikhli 443201,
Buldhaana
The pprimary justificaation for the deccade was to impprove the health of people,
primarily children, who suffeer because of inaadequate and coontaminated water supplies
and poor saniitation. In develloping countriess, three basic tyypes of the servvices could
benefit child health: an improvement in the quality drinkingg water, an incrreasing the
water provided and used and provision
p
of sannitation facilitiees for safe
quantity of w
disposal of thhe human excretta. Diarrhoea caan occur followiing the ingestion of water
contaminated with the infectiious agent of thhe diarrhoea. Waater containing pathogenic
p
bacteria at doses below thosee necessary to innfect humans, m
may be used for preparation
p
which time the bacteria
b
may inccubate and multtiply in the foodd. Effective
of foods, at w
disposal of huuman excreta sho
ould play a role in
i the control off major infectiouus agents of
diarrhoea whiich are eliminateed via the faces. Young childrenn, the primary excretors of
these agents, do not the toilet. Therefore, the hygienic ddisposal of theiir faces is
necessary to bbreak the faecal oral transmissioon of these pathhogens. Interrupttion of this
transmission bby water and saanitation improvvement is probaably the major mechanism
m
whereby children’s health can
c
be improveed. The bacteriiological examiinations of
udy. Excreta dissposal appears too consistently play a more
drinking wateer support the stu
important rolee in determinin
ng children’s heealth in developping areas, thann do water
supplies especcially where the prevalence
p
of diiarrhoea is high.
Published on 25tth February 2011
Page 6
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
P
PP05
Charaacterization of
o Contaminants of Cosm
metics and Th
heir
Antibacterrial Sensitivitty against Syn
nthetic and N
Natural Preseervatives
Ayushi. A. Chaurasiy
ya and R. B. Va
aidya
ayushi.chaurasiya
a
a@gmail.com
Deppartment Of Miccrobiology, Instittute Of Science, M
Mumbai 4000322
Microbial contaminaants in cosmetiic products havve two origins i.e. during
production annd filling, and du
uring the use by the customer. F
From the momennt in which
the cosmetic unit is opened
d until the connsumer finishess the product, there is a
permanent, vaariable and addittive microbial coontamination of tthe cosmetic cauused by the
domestic enviironment and th
he consumer's body.
b
Contaminaating microorgannisms may
cause spoilagee of the productts and when pathhogenic represennt a serious heaalth risk for
the consumer worldwide. Theerefore, the studyy was conductedd to determine thhe level and
the type of coontaminants present in various intact and in usse products. Thee microbial
load was founnd to be higherr than the permiissible limit of 103cfu/g or ml for topical
products and 102cfu/g or ml for
f eye based prroducts as per B
Bureau of Indiann Standards
(2009). The coontaminants werre isolated, mainntained on tryptonne soya agar andd identified
on the basis off morphological and biochemicaal characteristics. The biodegradaable ability
of isolates (200) were studied in
n presence of thee preservatives ssuch as methyl paraben
p
and
propyl parabeen as sole sourcces of carbon added
a
separatelyy to mineral salts medium
during successive enrichmentts. The biodegraadation of preserrvatives was connfirmed on
medium by obseerving zone of hydrolysis
h
for eiight isolates. Thhe effect of
mineral salts m
various conceentrations (0.2-2
2%) of propyl paraben was sttudied colorimeetrically on
growth of fouur isolates and it
i was observedd that the growtth was slightly affected
a
in
presence of prreservative as co
ompared to in its absence. The pplasmid curing experiment
e
established thhat the property
y of utilization of
o the paraben is chromosomaal encoded.
Thus, it was noted that the preservatives were
w
not successsful in preservattion of the
m of antibacteriial activity. Now
w days, the
cosmetic prodducts in respect of their spectrum
use of essential oils as functional ingredientss in cosmetics iss gaining momenntum, both
c
in inngredients from
m natural sources and also
for the growiing interest of consumers
because of inccreasing concern
n about potentially harmful syntthetic additives. Therefore,
the antibacteriial activity of su
uch 11 essential oils
o was screeneed against the 20 isolates of
the contaminaants and 6 of them
t
were founnd to be antibaacterial in action showing
variation in ddegree of inhibiition; while 2 of
o them showedd no antibacteriaal activity.
However, lim
me, eucalyptus an
nd tea tree oil exhibited
e
inhibiitory action agaiinst all the
isolates. Concclusively, it wass established thaat the natural esssential oils werre better in
spectrum of acction.
Published on 25tth February 2011
Page 7
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
P
PP06
Study of Q
Quorum Sensing and Quorum Quench
hing in Vibrio fischeri
and Application
A
i Aquacultu
in
ure
Archa
ana J. Marry and
a
Jyoti Man
ntri
Sophia Collegge, Mumbai
n in the world market
m
is estimateed to be above 110
1 million
Totall fish production
tons and aquuaculture accoun
nts for 47%. However,
H
in most of the Asiann countries
aquaculture inndustry is beset by bacterial andd viral diseases, especially the shrimp
s
and
prawn culturee industry. Diseease has becomee a limiting facctor in prawn and
a shrimp
culture subsecctor. Major aqu
uaculture pathoggens are Vibrio harveyi & Vibrrio fischeri
which are biooluminescent baacteria commonnly found in aquuatic environmeent. So far
conventional approaches likee use of disinfeectants and antim
microbial drugss have had
a
diseasess. The use of anntimicrobial
limited successs in the prevention or cure of aquatic
drugs has ledd to emergencee of more viruulent pathogens and eventual transfer
t
of
antimicrobial resistance to hu
uman pathogenss. In this study, we tried using,, beneficial
bacteria (probbiotics), which are environmenntally safe to diisplace the pathhogens and
inhibit their prroliferation. Bio
oluminescent baccteria were isolaated from squid .They
.
were
confirmed as Vibrio by platin
ng on Thiosulphhate citrate bile sucrose agar annd studying
V
fischeri bby biochemical tests .This
their morphollogy. They weree identified as Vibrio
culture showeed maximum bio
oluminescence inn 10 hours whenn grown in sea water
w
broth
.When lumineescence induced cell free supernnatant broth was added to a freshh sea water
broth +Vibrioo culture it was
w observed thhat luminescencce occurred witthin 6 hrs
indicating quoorum sensing due
d to autoinduuction,. Five isoolates were obtaained from
shrimp’s intesstine & identified
d tentatively as belonging
b
to Baacillus species. When
W
a cell
free filtrate off a cocktail of th
hese 5 isolates was
w added to the Vibrio culture inn sea water
broth luminesscence was delaayed indicating quorum quenchhing. All these 5 isolates
could inhibit the Vibrio isolaate when testedd by cross-streakking method. Antagonism
A
hod, using indivvidual cell free eextract of the fivve Bacillus
assay, by welll-diffusion meth
mm, 13mm,
isolates againnst Vibrio culturre showed zonees of inhibition of 12mm, 15m
14mm, 16mm
m. To check the antagonistic
a
poteential, co-culture method was perrformed by
growing Vibriio and the Bacilllus culture togeether & it was fo
found that viablee counts of
Vibrio decreassed after 6 hrs & there was no grrowth at 48 hrs. The Bacillus isoolates were
found to be nnon-hemolytic when
w
grown on blood
b
agar thus can be safely used
u
in the
environment. The Bacillus iso
olates can thus be used individdually or as a coocktail as a
probiont to ovvercome Vibrio in
nfection in shrim
mp aquaculture.
Published on 25tth February 2011
Page 8
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP007
Biodegradation
n of Triaminootrinitrobenzzene (TATB)
Abhijit Mo
ore, Sayali Ma
ahajani, Seem
ma Sarnaik an
nd Pradnya Kanekar
K
Microbial Sciences Diivision, Agharkaar Research Instiitute, Pune 411 004
0
Envirronmental contaamination of nitrrocompounds is associated princcipally with
the explosivess industry. However, global prooduction and expplosive use is unnavoidable.
The nitroexpllosive as well as
a their environnmentally transfformed productss are toxic
showing sym
mptoms as kid
dney trouble, jaundice etc. Present studiees include
biodegradationn of recently developed
d
insennsitive high eneergy nitroexplossive (IHE)
1,3,5-triaminoo-2,4,6-trinitrobeenzene (TATB)). It is an aroomatic explosivve, having
benzene ring structure with three
t
nitro grouups (NO2) and tthree amino (NH
H2) groups
ng. The current process for the production of TATB
T
used
attached, alterrnately to the rin
by HEMRL, P
Pune is a two-stage process usiing 1, 3, 5-trichhlorobenzene (TC
CB) as the
starting mateerial. In firstt nitration steep, trichlorobenzene is connverted to
trichlorotrinitrriobenzene (TCTNB) which thhen is further converted into TATB in
amination stepp. Wastewaters generated
g
duringg their productioon are highly aciidic having
high nitrate, aammoniacal nitro
ogen and chloridde content causiing environmenttal hazards.
TATB is know
wn as a recalcittrant compound because of the symmetric locaation of the
nitro group on the aromatiic ring, an arrrangement that limits attack of classic
mpounds. In
dioxygenase eenzymes involveed in the microbial metabolism oof aromatic com
the present stuudy, total 18 baacterial isolates were
w
obtained frrom soil samplees collected
from TATB pproduction site using
u
enrichmennt, adaptation annd soil baiting techniques.
t
Out of these, only two bacteerial isolates annd one isolate oof actinomycete could use
Davis Mingioli’ss synthetic
TATB as solle source of nittrogen when inccorporated in D
medium at thee concentration of
o 100 mg/l. Thee isolates showeed removal of TA
ATB in the
range of 20-330% and nitratee in the range of 20-35% whhen incorporatedd in Davis
Mingioli’s synnthetic medium supplemented
s
w 0.05% peptone.
with
Published on 25tth February 2011
Page 9
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP008
Production of Am
mylase by Bacteria
B
Isoolated from Soil
Samp
ple
Bedare Monika and Madhuri
M
Amru
utkar
Departmeent of Microbiollogy, Modern Coollege of Arts, Sccience and Comm
merce,
Shivajinagar, Pune
P
411 005
Starcch degrading baacteria are most important forr industries suchh as food,
fermentation, textile and pap
per. Thus isolatting and manipuulating pure cuulture from
d importance for various biotecchnology industrries. In the
various samplles has manifold
present investtigation bacteriaal strains were isolated from sooil sample colleected from
Agricultural C
College, Pune. Si-5 & Si-6 bacteerial isolate from
m soil were founnd to be the
most promisinng amylase produ
ucing bacteria am
mong 15 isolatess & these were selected
s
for
further study. Growth patterns as well as optiimum growth coonditions were determined.
d
w 37oC, whereeas maximum growth
g
was
The optimum temperature forr these strains was
oncentration resppectively. The pH
H range was fouund to be 7observed at 1aand 2% starch co
8 for optimum
m growth. Maxim
mum enzyme actiivity was observved at this condittion.
Key Woords
Amylase,
A
soil, staarch degrading
Published on 25tth February 2011
Page 10
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP008
Antiplasmid Activity of Herbal Exxtracts on M
Methicillin Ressistant
Staphylococccus aureus
A.D Bholay *, Katkad
de A.N,Paleka
ar K.S.
adbholay@ggmail.com
Department off Microbiology, K.
K T. H. M. Colleege, Nashik
methicillin resisttance Staphylocooccus aureus (M
MRSA) is a seriious health
The m
problem and m
major challengee to the global drug
d
discovery pprogrammers. Most
M
of the
genetic determ
minants that con
nfer resistance too antibiotics aree located on R-pplasmids in
bacteria. The present investig
gation was underrtaken to investiigate the ability of organic
ves of different plants to cure R- plasmids froom certain
and aqueous extracts of leav
clinical isolatees. Active fractiions demonstrating antibacteriall andant plasmidd activities
were isolatedd from the org
ganic and aqueeous extracts oof shade dried leaves of
Azadirachtainndica, Annonasq
quamosa, Ocim
mumsanctum, P
Pongamiapinnataa. Plasmid
curing activityy of organic exttracts wasdeterm
mined by evaluatting the ability of
o bacterial
growth in the presence of anttibiotics. The phhysical loss of pplasmid DNA inn the cured
med by agarose gel electrophoreesis.The active fractions
f
of
derivatives waas further confirm
aqueous and organic extracts of leaves of tested plants cuured the R-plasmids from
MRSA. Such plasmid loss reversed the muultiple antibiotic resistance in curedstrains
making them sensitive to low concentrations of conventionall antibiotics. Theese extracts
op antiplasmid agent of natural origin to control
c
the
may be a soource to develo
development aand spread of plaasmid borne mulltiple antibiotic rresistance.
Key Woords:
Antibacteriaal Activity, Antib
biotic Resistancee, Antiplasmid A
Activity, Herbal Extracts,
Plasmid Curing
C
Published on 25tth February 2011
Page 11
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP009
Characterization of Thermo-Tole
T
erant and Aciid /Alkali Tolerant
β-Glucosidase from
m Bacterial Isoolate
Bhalkikar S.
S B., Diwan V.
V A.1, Ranjeka
ar M. K..2
1
2
D
Department of Microbiology,
M
Daayanand Science College, Latur
Heaad, Department of
o Microbiology, Dayanand Scieence College, Laatur
Celluulose is most abu
undant on earth constituting
c
abouut half of the carrbonaceous
compounds inn terrestrial bio
omass. Endo andd exo glucanases act synergisttically and
promote soluubilization of crystalline
c
celluulose into soluuble sugars. Biotechnical
application off β glucosidase include
i
convertiing phytoestrogeen glucosides inn fruits and
vegetables to aglycone moieeties, detoxificattion of cassava,, aroma enhancement and
removing bitteer compounds frrom citrus fruit juices or unripe olives. The purppose of this
study is to isoolate bacteria fro
om raw materiall that is rice straaw and unai therrmal spring
water. These isolate would be screened forr β glucosidasee production andd different
parameters off these crude filtrrate would be dettected.
Key Woords
Thermo-Tolerantt
Celllulose, β-Glucosidase , Acid /Alkali Tolerant, T
Published on 25tth February 2011
Page 12
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP110
Detoxicaation of Azo Dyes
D
by Micrrobial Pure C
Cultures as well
w as
Microbial Consortia
C
Bhola
ay A. D., Maid
d Sneha A., Bo
orse Priti k., R
Rakte Snehal B.
Department of Microbiology, K.T.H.M Collegge, Nashik.
Azo dyes are used widely
w
in the mannufacture of varrious consumer goods
g
such
per, hair care prooducts and cosm
metics. Azo dyes are largest
as leather, textile, plastics, pap
m
than half oof the annual prooduction. It
group of dyess used in industrry representing more
has been estim
mated that 10% of the dye stufff during this dyinng processes dosse not bind
to the fibers aand is therefore released in to thhe sewage or ennvironment. Azo dyes have
structural propperties that are not easily degraadable under naatural conditionss. Different
microorganism
ms such as aero
obic and anaerobbic bacteria andd actinomycetes have been
found to cataalyze dye decolo
orization and dyye detoxication. From textile efffluents 25
microbial speecies were isolatted. After screeening the most efficient four cultures are
mycetes. All
selected for fuurther study out of them two arre bacteria and ttwo are actinom
four species ccan decolorize and detoxify ‘A
Acid dyes’ (Metthyl red), ‘Reacctive dyes’
(Reactive red--195, Reactive black-5,
b
Reactivve yellow-145), ‘Tryphenyl meethane dye’
(Malachite grreen) and ‘simu
ulated effluent’ (Mixture
(
of 10 dyes). In ordeer to study
physiological and metabolic aspects
a
of decoloorization and dettoxication processs Reactive
was selected as model
m
azo dye under
u
static andd shaking condittions. After
yellow- 145 w
optimization of physicochem
mical parameteers bacterial puure cultures shhows 90%
d
under statioonary conditionss and Actinomyycetes pure
decolorizationn within three days
cultures show
ws 80% of decolo
orization withinn 5 days under sshaking conditions. Instead
pure cultures tthe bacterial and
d actinomycetal consortia are alsso tested for deccolorization
and detoxicatiion. Promising results
r
were obtaained about 95%
% to 96% of deccolorization
seen within lesss period of timee than that of purre cultures. The bbiodegradation of
o azo dyes
produces variious ‘aromatic amines’ which were confirmeed by ‘TLC’ and
a
‘FTIR’
studies. Detoxxication studies were carried ouut in vivo by seeed germination. The mixed
consortia provved to be significcantly efficient than
t
the individuual isolates, whicch could be
further characterized by activaated sludge proceess for heterogenneous dye efflueents.
Key woords
Detoxiccation, Azo Dyess, Reactive Yelloow, Consortia, F
FTIR, Decolorizaation
Published on 25tth February 2011
Page 13
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP111
Probiotic P
Properties off Lactobacillu
us Isolates froom Milk of Domestic
D
Animals aand Commercial Availablle Probiotic P
Preparations against
nteric Bacteriial Pathogenss
En
Bhutad
da S. A.1 and Tambekar D.. H.2
1
sabhutada13@
@gmail.com
Department oof Microbiology, New Arts, Com
mmerce and Scieence College, Ahhmednagar,
2
Departm
ment of Microbiology, S.G.B. Am
mravati Universitty, Amravati, 4444602,
p of microbes thhat may be helpfful directly for enhanced
e
Probiiotic is the group
resistance agaainst intestinal pathogens
p
and inn the preventionn of diseases. A total of
120 milk sam
mples (40 each from buffalo, cow
c
and goat) were analyzed and 110
isolates were identified as Lacctic Acid Bacterria (LAB). Out oof these 11 isolaates were
recognized ass prominent prrobiotics, amongg them 3 isolaates were identtified as
excellent proobiotics. These excellent probbiotics were coompared their probiotic
p
potential withh commercial pro
obiotic preparations such as Spoorlac powder, LaactoBacil
plus, P-Biotics kid, Gastrolinee, Pre-Pro kid annd standard probbiotic bacterial strains
s
L.
MTCC 2621) an
nd L. rhamnosus (MTCC 10448). The isolated LAB
plantarum (M
exhibited excellent probiotic characteristics than commerciaal probiotic prepparations
and standard pprobiotic bacteriial strains. Studyy suggested that use of probioticc bacteria
from milk off domestic anim
mals can be help to prevent oor control the intestinal
i
infections andd contributes heaalth benefits to coonsumers.
Key Woords
Probiotics, L
Lactobacillus, Antibacterial
A
Actiivity, Bacteriociin, Commercial Probiotic
P
Preparattions
Published on 25tth February 2011
Page 14
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP112
Isolaation, Purifica
ation and Ap
pplication of L
Lipase Enzym
me
Bhadale S. V., Bhalera
ao M. N., Patiil N. N., Ghule
e P. V. and Ku
ulkarni
bhadalesujeet123
b
3@gmail.com
gy, Annasaheb Magar
M
College, H
Hadapsar, Pune 411028
Departmennt of Microbiolog
t food, dairy, detergent,
d
cosmeetic, tanning inddustries and
Lipasses are used in the
most widely uused as biocataly
yst in the field of organic chem
mistry. In the vieew of their
current and ppotential application lipases arre considered tto be promisingg class of
industrial enzzyme. These arre currently used in the prodduction of fine chemical,
pharmaceuticaal preparations, flavour compoounds and otherr food additativves. Lipase
(triacylglycerool acylhydrolasee; EC 3.1.1.3) arre produced by aactinomycetes either
e
alone
or together wiith esterases (carrboxylic-ester hyydrolases; EC 3.1.1.1).Lipases are
a unusual
extracellular, hydrolytic enzym
me because theyy act on substrattes providing ann water and
Even though Lipaases and esterasees differ in theirr ability to act at Interfaces.
oil interface.E
In general, thee use of water so
oluble substrates is considered diiagnostic for esterases, and
the use of waater in-solublesub
bstrates is consiidered diagnosticc for Lipases Sccreening of
lipase produceers is done by ussing tributyrin(9)) or Tween 80(12) as a substratee .Olive oil
is an inexpeensive substitutee for trioleoylgglycerol. Then various concenntration of
tributyrin werre added to min
nimal medium annd activity weree studied . Tribuutyrin agar
and Rhodam
mine B agar teest was done foor confirmation. Rhodamine B is used in
presence of uuranyl ions, yieelding orange fluorencent
fl
comp
mplexes with an excitation
wavelength oof 350nm. Enzzyme purificatioon was done by Ammonium
m sulphate
precipitation m
method. Molecu
ular weight was determined
d
by S
SDS-PAGE. Appplication of
enzyme was sstudied. Most co
ommercially avaailable lipases arre synthesized by
b bacteria,
fungi and actiinomycetes. Thee search for new
w lipases with ggreater thermosttability and
various substrrate selectivity is still going on. Hence this worrk is being donee for lipase
producer by a actinomycetes from
f
hot water.
Published on 25tth February 2011
Page 15
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP113
Anttibiotic Produ
uction from Bacillus
B
spp. A
Active againsst
Staphylococccus aureus
Chavan K. J., Patil S. D., Ranjekarr M. K.
Post Graduaate Department of
o Microbiology,, Dayanand Scieence College Lattur, India.
The word antibiotic comes from thhe Greek anti m
meaning 'againstt' and bios
ny Bacteria can produce antibiootic. Among thesse Bacillus
meaning 'life'.. There are man
spp. Produce m
many type of antibiotics. Mersaccidin is a new peeptide antibiotic containing
-methyllanthhionine. It is claassified as a mem
mber of the prooposed lantibiotiic group of
antibiotics, annd is produced by
b a species of Bacillus.
B
It is peeptide antibiotic. This new
peptide antibiotic isolate from
m fermentations of a Bacillus sppecies. It is maainly active
m-positive bacteria, particulaarly Staphylocooccus aureus, including
against Gram
methicillin-ressistant strains.Th
his Meracidin anntibiotic is activve against manyy strains of
Staphylococcuus spp.
Key woords
Mersacidin, Baacillus spp.
Published on 25tth February 2011
Page 16
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP114
Antimicrrobial Activity
y of Mangrovves against M
Multidrug-Reesistant
(MDR) Paathogens
Bholay A. D., Kurup Sun
nila B., Vidha
ate Pushpa S.,, Rahatekar Rohini
R
R.
adbholay@ggmail.com
M. College,Nashiik.,Pune Universsity.
Departtment of Microbiology ,K.T.H.M
The term mangrovee is also used to designate F
Facultative halopphytic and
marine tidal forests comprissing of trees, shrubs,palms,
s
eppiphytes,ground ferns and
grasses whichh are associated in
i strands or grooves.These wetlaand ecosystems are among
the most prodductive and diverrse in the world and more than 80% of marine catches
c
are
directly or inddirectly dependeent on mangrovee and other coasstal ecosystems worldwide.
w
Mangroves arre highly producttive and econom
mical which also protect the shorreline from
erosion and cyyclonic conditio
ons. This uniquee tree resource iss usedfor variouus purposes
like tannin exxtraction, paper and pulp, firew
wood, timber, chharcoal ,fodder, medicinal
uses and severral other by-products.The antim
microbial activityy of mangroves are largely
unexplored. T
The most dominaant mangrove sppecies found aloong the east and west coast
of India are :- Rhizophora mucronata, R.aapiculata, Sonneeratia alba, S. caseolafis,
Xylocarpus ggranatum, X.molluscensis, Aviccennia officinaliis, A. marina, Bruguiera
gymnorrhiza, B.parviflora, Salvadora
S
persicca, Sesuvium poortulacastrum. Mangroves
M
and mangrovve associates contain biologgically active aantiviral,antibaccterial and
antifungal com
mpounds. They also
a provide richh source of sterooids , triterpeness, saponins,
flavonoids, alkaloids and tann
nins. Therefore it is worth to sccreen mangrovee plants for
the presence of new antibaccterial compoundds to combat thhe pathogenic bacterial
b
&
fungal strainss. The MDR pathogens
p
like Streptococcuss , E.coli , Kleebshiella ,
Pseudomonas , Staphyllococcu
us aureus , Staphhyllococcus xyluulose , Xanthomoonas which
were resistannt to drugs lik
ke Amicasin, Netilmycin , G
Gentamycin, Cefotaxime,
C
Amphicillin +
+Sulbactam,Spaarfloxacin were treated with tthe mangrove extracts
e
at
different conccentration in two
o different solvennts (EA, MeOH
H) in Muller-Hinngton Broth
,become sensiitive to the above mentioned druugs.Extracts preppared in Ethyl accetate (EA)
are more potennt as compared to
t that prepared in Methanol (M
MeOH). Sensitivitty of MDR
pathogens is ccarried out in Mu
uller –Hington agar
a
by Agar Gell Diffusion Methhod. Extact
of mangrove w
was obtained by
y Sequential Soxxhlet Extraction method. Most of
o the drug
resistance prooperties are associated with R –plasmids.The
–
phhysical partial or
o complete
loss of plasm
mid were confirrmed by Agarrose gel electroophoresis technnique. The
synergestic eeffect of mixed
d extracts and the potentiatioon activity withh standard
antibiotics aree under experimeentation.
Key Woords
Mangroves, M
Multidrug-Resisttance, Unexplored, Synergestic E
Effect, Potentiattion Effect
Published on 25tth February 2011
Page 17
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP115
Biosorptioon of hexavalent chromiu
um from aqueeous solution
ns using
dead fungal biomass of
o P. chrysoggenum
Dee
ep.V.Barot & Varsha.Vaidy
V
ya
barot_deep@yyahoo.co.in
Departmen
nt of Microbiologgy, Institute Of S
Science,
15 Maadam Cama Roaad Mumbai-4000032
Chroomium presentss threat to thhe environmennt due to itss toxicity,
bioaccumulatiion and biomaagnifications heence necessitating its removaal. Use of
microorganism
ms for removal of Chromium by biosorption is gaining attenntion. This
research emplloyed dead biom
mass of P. chryysogenum in battch experiments where the
effects of pH, temperature, shaking speed, metal
m
concentrattion, mass of biiomass and
were examined. pH was the most critical factorr increasing the removal
r
of
contact time w
Chromium w
with a decrease in pH.
1
by
Biossorption was ennhanced from 13.19%
treatment of bbiomass using 1M
1 phosphoric acid
a
coupled wiith autoclaving to
t 22.93%.
The optimum temperature and
d shaking speed for the biosorpttion were found to be 25°C
he removal of Chhromium was afffected by conceentration of
and 150 rpm rrespectively. Th
the metal as w
well as biomass. The biosorptionn reached equilibbrium after 165 min of the
initial contact exhibiting 1st order
o
kinetics. Sorption
S
data weere described by freundlich
and Langmuirr Isotherms. Deesorption and ree-usability studiees indicated useefulness of
10mM NaOH
H at S/L of 2 for
f five biosorpption -desorption cycles. The amine and
carboxyl grouups of the fungall cell wall contribbuted to the sorpption process as confirmed
from potentiometry and FT-IR
R analysis. SEM
M indicated accuumulation of Chhromium in
the fungal mycelium.
Published on 25tth February 2011
Page 18
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP116
Study oof Antibiotic Resistance
R
Paatterns in Caampylobacterr spp.
Isolatted from Pun
ne, Maharash
htra
De Souza N. A.1, Kamble
K
A.2, Parkar S. D.1, Kapadnis B. P.
P1
bpkap@unippune.ac.in
Departmeent of Microbioloogy, University oof Pune
2
Institute oof Bioinformaticss and Biotechnology, University of Pune, Pune 411
4 007
1
Camppylobacter are thermophilic
t
Graam negative enteric pathogens responsible
r
for both human and animal disease.
d
Poultryy is one of the m
most important sources of
human Camppylobacter infecctions. Antimicrobial resistannce has emerged among
Campylobacteer mainly as a consequence of the
t unchecked uuse of antibioticss in poultry
production. C
Campylobacter jejuni
j
and Cam
mpylobacter colii have been foound to be
commonly asssociated with baacterial diarrhoeea. C. jejuni acccounts for moree infections
than those cauused by Salmon
nella and E.coli combined. Exccept for certain severe and
systemic casess, most cases off campylobacteriosis do not requuire antimicrobiaal treatment
as they are self-limiting. Maccrolides and fluooroquinolones arre regarded as thhe drugs of
choice to treatt such infectionss. Campylobacter shows intrinsicc resistance to a number of
antibiotics inncluding trimeth
hoprim, vancom
mycin and cefooperazone. In this
t
study,
Campylobacteer isolates were studied for theeir antimicrobiall susceptibility pattern.
p
25
antibiotics bellonging to the classes
c
macroliddes, fluoroquinoolones, cephalosporins and
aminoglycosiddes were selecteed for the study. They included erythromycin, ceftriaxone,
c
ciprofloxacin, gentamicin and
d tetracycline. Thhe cultures used were isolated froom poultry
ne, Maharashtraa. The minimum
m inhibitory conncentration
slaughter houuses around Pun
(MIC) as welll as the minimum
m bactericidal cooncentration (MBC) was determ
mined using
the agar diluttion and broth microdilution
m
meethods. The MIC
C values were determined
d
according to breakpoints sp
pecified by CL
LSI (Clinical aand Laboratory Standards
Institute). Breeakpoints for ressistance to ciproffloxacin are estaablished at ≥4 µg/ml while
values for azitthromycin and erythromycin
e
aree ≥8 µg/ml and ≥32 µg/ml resppectively. It
is also necesssary to determin
ne whether the resistance to thhe antimicrobiall agents is
plasmid or chhromosomally mediated
m
by studdies involving pplasmid curing. This work
aims at stuudying the preevalence of anntibiotic resistaance in Camppylobacter.
Key Words
Agar D
Dilution, Antibiottic Resistance, Broth
B
Microdiluttion, Campylobaacter
Published on 25tth February 2011
Page 19
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP117
Antimicrobial Activity
y of New Sulp
phanylamidee Analogues against
a
Commonlyy Occurring Multidrug
M
Reesistant Urin
nary Tract Paathogens
Gauri Devasthale1, Alok
A
Vyas2, Divya Nair1 and
d Jhilmil Gho
oshal1
dgauri1972@yyahoo.co.in
Post grraduate department of Microbiollogy, Abeda Inam
mdar Senior Colllege,
Azam
A
Campus, Camp,
C
Pune- 1
2
Research Ceenter in Chemisttry, Abeda Inamddar Senior Colleege, Azam Campus, Camp,
Pune-- 1
1
Elimiination of the most
m
widespreadd multidrug resisstant strains of pathogenic
bacteria like SStaphylococcus, E.coli and Pseudomonas has beecome a major chhallenge in
the field of m
medicine. These strains have accquired resistancce to most of thhe existing
antibiotics speecially sulphonaamides. They aree also responsiblle for many of thhe hospital
acquired infecctions. It is thuss the need of thee hour to develoop new more efffective and
safe drugs to fight these no
osocomial pathogens. Sulphonam
mides have beeen used as
effective antibbacterial agentss for many yearrs and are welll known for theeir diverse
pharmacologiccal activities. Seven new compounds
c
werre
synthesiized from
sulphanylamidde and structurally characterizzed. The sulfonamide- Schiff bases
b
with
biologically acctive Gallic acid
d moiety were incorporated intto these moleculles in order
to increase thheir antimicrobiaal spectrum. Thhe side chains w
were substitutedd with new
active groups.. The compound
ds were named as
a STZ, IND, TH
HB, DHB, CHR, SNL, and
SDZ. Our chiief aim was to check
c
the activitty of these newly synthesized compounds
c
against some of the most commonly occurrring multidrug resistant pathoogens from
pathological ssamples. . The organisms werre isolated from
m urine sample and were
identified as E
E.coli, Staphylo
ococcus and Pseeudomonas. It seeems that these organisms
develop resisttance to sulphon
namides rapidly and
a efflux pumpps are considered to be the
most commonn and basic mecchanisms of muultidrug resistancce. One or more than one
pump may bee present in the resistant cells. The newly synnthesized compoounds were
assayed by w
well diffusion meethod for antim
microbial activityy against these pathogens
both clinical iisolates as well as standard culltures. Among thhe tested compoounds STZ
exhibited a ggood antibacteriial activity. MIIC and MBC w
was also performed. Cotrimoxazole w
was used as a stan
ndard.
Keywords:
Multidrug R
Resistant Pathogeens, Antibacteriaal Activity, STZ , Sulphanylamidde, UTI ,
Schiff Bases
B
Published on 25tth February 2011
Page 20
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP118
Isolation
n, Identificatiion and Charracterization of Biolumineescent
Bacteria frrom Fish Sam
mple and Its Use
U as Waterr Pollution In
ndicator
Gauri Paw
war, Deepali Mane,
M
Vyanka
atesh M. Sana
am, Shilpa Mu
ujumdar
and Smita Bhuyan*
B
Departm
ment of Microbiollogy, Modern coollege of Arts, Sccience and Comm
merce,
Shivajinagar Puune 411 005
Bioluuminescence is the light emisssion phenomennon in which photons
p
of
specific wavellengths are emittted due to conveersion of chemicaal energy into ligght energy.
Some marine fishes have evolved the ability, to harness thesee light-producingg microbes
on. These animaals have speciall light organs thhat provide
showing symbbiotic associatio
bioluminescennt bacteria both a safe place to live and a sourrce of food. Graam Positive
luminescent ddiplococci weree isolated from marine squid. Effects of envvironmental
parameters suuch as temperatu
ure and pH weree observed on prroduction of lum
minescence.
Maximum lum
minescence wass observed at 2260 C and at pph 8. The enzyymes were
produced by ddiplococci are caatalase, oxidase and
a esterase.
Key woords
Bio
oluminescence, Squid, Esterase
Published on 25tth February 2011
Page 21
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP119
Assessmen
nt of Geneticc Diversity am
mong Mango Varieties byy RAPD
Mark
kers
Hris
shikesh Joshi¹, A. R. Desaii²
1
Department oof Biochemistry, New Arts Comm
merce and Sciencce College, Ahm
mednagar
2
ICAR, Ela, Oldd Goa, Goa.
Inforrmation about the extent of genetic diverssity/relatedness in mango
germplasm is vital for develo
oping coherent strategies
s
for fuuture gains in prroductivity.
ness among manggo cultivars deveeloped in Goa haas not been
The genetic diiversity/relatedn
investigated ppreviously. We have assessed the genetic divversity among 16 mango
cultivars usinng randomly am
mplified polymorrphic DNA (RA
APD). 25 randoom ten-mer
primers were surveyed, out of which 15 were
w
selected foor further studiees. Genetic
ween genotypes was in the rangee of 0.10 to 0.66%
%. These coefficcients were
similarity betw
utilized to connstruct a dendog
gram using the unweighted
u
pair group of arithm
metic means
(UPGMA). T
The genotypes were
w
grouped innto two main cllusters. But no significant
segregation am
mong varieties found
fo
may be duue to less geograpphic distance annd common
gene pool origgin.
Key woords
RAPD, Germplasm, UP
PGMA, Dendogrram.
Published on 25tth February 2011
Page 22
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP220
Development of Micrrobial Biostim
mulants to En
nhance Fertility of
Saline So
oils for Sustaainable Agriculture
Indulkar V..S.* Siddiqui A.G.
A 1, Ranjek
kar M.K.2
1.
2.
D
Department of Microbiology,
M
Day
ayanand Science College, Latur.
Headd, Department off Microbiology, Dayanand Sciennce College, Latuur.
Biosttimulants is a claass of product which
w
enhances soil fertility as well
w as plant
growth. Biostiimulants enhancces shoot and rooot development, improve soil strructure and
texture,improvve plant ability to recover from
m disease and innsect damage, reduces
r
the
effect of pH and soil colloid
dal imbalance. Various
V
microorrganisms contribbute to soil
m
phosphate solublizing
fertility whiich are nitrogen fixing microorganisms,
microorganism
ms, siderophore producing micrroorganisms, fuungi, nematodes, protozoa,
bacteria livingg around root system. Presentt work is aimedd to isolates saalt tolerant
microorganism
ms as well as saalt tolerant Azotoobacter species from saline soiil and their
use as biostim
mulant to enhancee fertility of salinne soil.
Key Woords
Soil Fertilityy, Saline Soil, Saalt Tolerant Miccroorganisms, Saalt Tolerant, Azottobacter,
Biostimuulants
Published on 25tth February 2011
Page 23
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP221
Assessm
ment of Waterr Purification
n Potential off Moringa Ole
leifera
Co
oagulant Prottein (MOCP))
Jain Ree
ena R. and Ka
adam Shradhha D.
@gmail.com
reenajain512@
Depaartment of Micro
obiology, Mooljii Jaitha College,, Jalgaon-4250002
Moriinga oleifera (M
MO) is a multipuurpose, medium
m or small-sized tree, from
regions of north-west India and
a indigenous to
t many parts oof Asia, Africa, and South
America. Its ppods have been employed
e
as an inexpensive andd cost effective sorbent for
the removal oof organics, and
d coagulant for water treatmentt. It is a non-toxxic natural
organic polym
mer. Treatment plants generally uses materiaals like synthettic organic
polymers and inorganic chemiicals which are not
n that much cost worthy and are
a causing
man health. So,th
he main objectivve of this work w
was to use the MO
M seeds as
hazards to hum
a natural,cost effective,eco friiendly adsorbentt for the examinning the quality of
o drinking
ga oleifera seedds were investiggated as alternattive natural
water of our campus. Moring
materials for ddrinking water trreatment. The cooagulant protein from the Moringga Oleifera
seed was extrracted and then
n purified on thhe lab scale by using dialysis technique.
Moringa Oleif
ifera coagulant protein (MOCP
P) possessed connsiderable coaguulation and
sludge condittioning propertiees as alum. It also
a
showed anttimicrobial effects against
microorganism
ms at room tem
mperature. It also inhibits the bacteriophage replication
.MOCP was ffound to be stablle at pH 6.8.A sm
mall volume coaagulation methodd was used
to verify the ccoagulation activity experimentts. We conclude that the MO biiomass has
the potential tto be used in th
he wastewater treatment in an eefficient way andd with low
cost.
Key Woords
Moringa oleeifera , Moringa oleifera Coagulaant Protein (MO
OCP), Coagulatioon, Alum.
Published on 25tth February 2011
Page 24
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP006
A Cost-Effe
fective Protoccol for Cultivvation, Microbial and Biocchemical
Analysiis and High Yielding
Y
Nutrritious Varietty of Mushroooms.
* Jogii M. M., Surya
awanshi Suprriya V., More B
Bhagyashree
e V.,
Anandwani Nilesh P., and
a
Patil Jaye
esh A .
mopa2005@reddiffmail.com
* Departmentt of Microbiology
gy, PSGVPM’s, ASC
A College Shaahada, 425409, Nandurbar
N
Mushhroom is a flesshy, spore-beariing, fruiting boody of a fungus typically
produced abovve the ground on
n soil or on agroo industrial wasttes. In our project we have
cultivated andd analysed the species of Muushrooms Pleurotus Sajar-Kajuu which is
popular for thheir nutritional value. For the cuultivation purpose. Well sterilizeed substrate
was used and temperature of 18-320C annd humidity of 80-85% was maintained
m
Mushrooms werre harvested onn 21st days. A
Another promising feature
throughout. M
observed wass regarding the inflorescence. An average off 10-12 flowerss has been
reported but thhe count obtainaable in the preseent study was uppto 20 flowers. The
T weight
of the flower nnormally attaineed is within 60-100 g. The wet w
weight reached inn this study
was 77 g and tthe dry weight was
w 11 g which was
w quite satisfacctory. We have done
d
micro
flora of mushhroom sample. We have biochhemically analysed the mushrooom which
mainly includdes proteins, carrbohydrates, Fat,, amino acids annd minerals whhich mainly
includes K,M
Mn,Cu,Na,Zn,Ca. ( AAS and Fllame Photometeer ) .We intendd to go for
further optim
mization of cond
ditions which iss likely to givee better yield inn terms of
inflorescence and weight of th
he fruiting body.
Key woords
Mushroom, Fungiculture,
F
AA
AS and Flame Phhotometer
Published on 25tth February 2011
Page 25
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP005
Biotraansformation
n of Textile Azo
A Dyes by A
Aerobic Bacteeria
Ka
ale R. V. and Thorat,
T
P. R.
ravimicro3@g
gmail.com , prakkash_micro1@yyahoo.co.in
P.G. Departm
ment of Microbio
ology and Reseaarch Center, Shri. Shivaji Mahavvidyalaya,
Barshi – 413 411,
4 Solapur
Releaase of azo dye in the environm
ment is of greaat concern due to, colour,
toxicity, mutaagenicity and reccalcitrant nature of the dye, considerable attentioon has been
given in deterrmining the abiliity of microorgaanism in decolorrization and degrradation of
the azo dyes. A
Acclimatized miicroorganisms issolated from natuural sources, weere used for
the study of ddecolorization an
nd degradation of the dyes Reacttive Orange 16 and
a Orange
2R. In all 12 ppromising isolatees of Reactive Orange
O
16 and 1 isolate of Orangge 2R were
isolated whichh could decolorize 1000 μg/ml of dye to moree than 70.00 % in nutrient
medium and up to 68.00 % in half strengthh nutrient mediium. The cell free
f
extract
6
% in less thhan 24 hours. Peercent decolorizaation of the
showed decoloorization up to 67.00
dye was deterrmined by specttrophotometer att (λmax) 492 nm
m and 484nm. Six
S isolates
reduced the COD by more thaan 85.00 %.
Published on 25tth February 2011
Page 26
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP222
Isolation of Bacteriop
phage againstt Campylobaccter from Pou
ultry in
Pun
ne
Ka
amble A.1, De Souza N.2, Pa
arkar S.2, Kap
padnis B.P.2*
1
bpkap@unippune.ac.in
Instittute of Bioinform
matics and Biotecchnology, Univerrsity of Pune. Puune
2
Department of
o Microbiologyy, University of P
Pune. Pune
Camppylobacter jejun
ni is a Gram-neggative food-bornne pathogen that is a major
cause of baccterial enteritis worldwide. It is a microaeropphilic and therrmotolerant
bacterium, coommonly found in poultry. Duee to the unrestricted use of anttibiotics in
poultry, antibiiotic resistance is
i rampant in Caampylobacter. Biocontrol of thiss bacterium
in live poultryy is difficult. An increasing trendd in antibiotic resistance is obserrved in this
bacterium. Phhage-therapy is a promising alternative
a
for C
Campylobacter biocontrol.
Bacteriophagees specific for th
his bacterium haave been isolateed previously buut no work
has been donee on it in India yeet. This study focuses on isolatioon of the phages from retail
poultry in Punne and their perccentage reductioon of target C. jeejuni strains. In the
t present
study 26 isollates of Campy
ylobacter were isolated from sslaughter housess in Pune.
Biochemical ttests were carrieed out along with a latex aggllutination test annd PCR to
confirm them
m. Alongside 6 bacteriophages
b
w
were
isolated frrom Chicken Inntestines to
combat the prresence of Camp
pylobacter jejunni which were foound to be Cam
mpylobacter
specific.
Key Woords
Campylob
bacter, Bacteriopphage, Phage-thherapy.
Published on 25tth February 2011
Page 27
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP223
Isolation
n and Identifi
fication of Patthogens of Seeafood Penaeeus sp.
*Ketki Nalawade
N
and
d Rajendra Ch
houre
D
Department of Microbiology,
M
Thee Institute of Scieence, Mumbai.
a in wide use and typical of the diet in
Freshh and frozen seaafood products are
Indian subconntinent. Prawns (Penaeus monoodon) were exam
mined for the presence
p
of
microbiologiccal contaminatio
on. Total bacteriial counts of aeerobic mesophillic bacteria
(AB), Salmoonella and Shigella
Sh
spp., Enterobacteriaaceae, Eschericchia coli,
Staphylococcuus aureus, Vibrrio spp and Pseeudomonas spp was done for 10 prawn
samples colleected from fish markets across Mumbai. The m
microbiological quality of
individual sam
mples varied wid
dely. The methood used for analyysis could servee as a basis
for future tessting of seafood
d, and as a tem
mplate for regionnal testing scheme on the
microbial conntamination of seafood for com
mparative epideemiological andd statistical
studies. Know
wledge of contam
minations is verry critical in thee Good Hygienicc Practices
programme annd effective hyg
giene as well sannitary proceduress in food processsing plants
are necessary,, should contamiination by colifoorm and faecal cooliform bacteria persist. 51
isolates were collected from these
t
experimennts which were tthen subjected too antibiotic
w found
susceptibility test for 16 antibiotics from varioous classes. 12 oof these isolates were
to be resistantt to 50% of the antibiotics usedd. It was found that 98.81% of the strains
were resistantt to at least onee drug. The inteensification of fi
fish culture has caused the
emergence off new pathogens,, and the need for
f sustainable trreatments and prrophylactic
measures. Theese 12 drug resistant strains weere treated with several herbal extracts to
provide an altternative to antib
biotics. These stuudies will help tto establish Goodd Hygienic
Practices to ensure pathogeen free sea foood supply. Inteernational interddisciplinary
d FAO, OIE and
a
WHO havee organized a number
n
of
cooperation iis essential, and
consultations to address the issues related to antimicrobiaal use, the emeergence of
otential public heealth impact.
resistant pathoogens and the po
Published on 25tth February 2011
Page 28
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP224
To Study A
Anti-Bacteria
al Activity of Various Med
dicinal Plantss against
Mixed Dental Flora
Kh
hadpekar A.*,, Thombre R.
Department of Biotechnologyy, Modern Collegge of Arts, Sciencce and Commercce, Pune 5.
The m
medicinal propeerties of several plants been doccumented in ancient Indian
literature. Thee purpose of thiss study is to evalluate the anti-baacterial effect of extracts of
13 medicinal pplants and to preepare a poly herrbal product in thhe form of tablet. Aqueous
and ethanolicc extracts of diifferent medicinnal plants weree screened for their antimicrobial actiivity on Blood Agar Medium. Agar well diffuusion method was
w used to
detect the zoone of inhibitio
on. Terminalia chebula, Caryyophyllus aromaaticus and
Garcinia indicca showed max
ximum zone of innhibition. The m
mixture of effectiive extracts
and many com
mmercial toothpastes were testeed against mixedd dental flora. The mixture
can prevent thhe growth of deental bacteria annd can act as ann astringent. Taablets were
prepared from
m the most effectiive samples.
Key woords
Herbal Extraacts, Anti-Bacterrial Activity, Aggar Well Diffusioon Method, Mixed Dental
Floraa.
Published on 25tth February 2011
Page 29
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP225
S
Screening and
d Production
n of Laccase ffrom Fungi
Kawade S.S.*,
S
Diwan V.A.
V 1, Ranjeka
ar M.K.2
1
2
D
Department of Microbiology,
M
Daayanand Science College, Latur
Headd, Department of Microbiology, Dayanand Sciennce College, Lattur
Laccase are multi copper oxidases having wide suubstrate specificcity mainly
mary carbon
found in whitee rot fungi. Lacccase act on broaad range of substrates. The prim
source would be used in placee of glucose & guaiacol
g
for lacccase production that
t
is rice,
o this study is to
t isolate fungi from industrial effluent &
wheat & maizze. A purpose of
woody part off dead plants. These
T
isolate wouuld be screenedd for laccase prooduction on
media containning guaiacol. Sccreened fungi would be tested fo
for production off laccase &
the effect of cculture filtrate on
n dye decolorizaation. Also charaacterization of thhese culture
filtrate wouldd be detected.
Key Woords
Lacttase, Fungi, Dye Decolourizationn.
Published on 25tth February 2011
Page 30
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP226
Studies on Bioemulsifier Production
n from Provdeentia Stuartiii Isolated
from Gard
den Soil
Lalit Lande, Smita
S
Bhuyan
n and Shilpa Mujumdar
lalitlande5532@
@gmail.com
Departm
ment of Microbio
ology, Modern coollege of Arts, sccience and comm
merce,
Shivajinagarr, Pune 05
Provddentia stuartii was isolated from garden ssoil of Modernn College,
Shivajinagar, Pune. It showeed good emulsiffication activityy and produced maximum
bioemulsifier of 237 EU/ml at
a 300C, PH 7.5 with
w (0.5%) sunnflower oil in Luuria Bertani
o showed produuction of urease and gelatinase.
broth. Provdenntia stuartii also
Published on 25tth February 2011
Page 31
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP227
Comparaative Study Of
O Bioinoculan
nts Prepared
d From Rhizoosphere
Soil And
A Non-Rhiizosphere Sooil.
M.
M S. Vrushali2, N. Rutika1
1.
saivrush.1989@
@yahoo.com
D
Department of biotechnology,
b
Sppicer Memorial College, Pune.
2.
Dr. D. Y. Patiil University
In thhis project a comparative study was carried out beetween the
bioinoculants prepared from rhizosphere soiil and non-rhizosphere soil. Thee screening
for nitrogen ffixing organism from rhizospheere and non-rhizzosphere soil waas done by
serial dilutionn using Ashby’s agar. Gram staaining and Capssule staining waas done for
confirmation oof organism. Plaant growth prom
moting rhizospherre properties were detected
by subjecting the organism to
t phosphate sollubalition using Pikovskayas aggar. Liquid
o ashby’s brothh using suitable strain and kept on shaker.
bioinoculants were prepared on
Organisms weere counted by Neubaur’s
N
chambber. Lab trials w
were done. The microbiota
of the rhizospphere was more active physioloogically than thaat of non-rhizossphere soil.
The root lengtth and the shoo
ot length of the plants
p
was foundd to be more in soil
s treated
with bioinocuulant prepared
d from rhizosphhere. This projeect will be appplicable in
increasing thee nitrogen conten
nt of the soil; phhosphate solubillisation increase phosphate
in soil and also productivity; Plant Growth Promoting
P
Rhizzosphere organissms release
p
As the rhhizosphere organnisms are more adapted to
amino acids, ssugars used by plants.
the environmeent giving high
h efficiency in terms
t
of yield oof crop such orgaanisms can
be isolated ffrom the soil an
nd custom-madde biofertilizers can be given to farmers.
Also, such biiofertilizers wou
uld not disturb the
t biological ccycles as is donne by other
chemical fertillizers.
Published on 25tth February 2011
Page 32
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP228
Isolation off Siderophorre Producing Organism frrom Rhizosph
here Soil
of Adathodda Vasica
Mad
dhuri Londhe,, Smita Bhuya
an and Dr.Shiilpa Mujumda
ar
Depaartment of Micro
obiology, Moderrn College Shivaj
ajinagar, Pune -55.
m rhizosphere soil
s
sample of A
Adathoda vasicaa (Adulsa)
Microorganisms from
were isolated on Luria agar. Twelve strainns were isolatedd. One Gram poositive rod
oduction by Arnnow and Caskys assay for Cataccholate and
Screened for sederophore pro
hore respectivelyy. Gram positivee rod showed Arrnow assay
hydroxymate type of sideroph
positive .Thiss organism takee for further sttudy ,Media opptimization for maximum
siderophore pproduction was carried out withh Succinate brooth, Luria broth,, King’s B
broth. It wass found that King’s B Media produces maxximum siderophhore. Other
Environmentaal factors pH 5..6, Temperaturee 280C, aerationn at 120 rpm were
w
found
optimum for m
maximum sidero
ophore productioon.
Published on 25tth February 2011
Page 33
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP229
Comparatiive Analysis of
o Faecal Miccrobiota from
m Normal an
nd Obese
Wistar Rats.
R
Mahesh R Ghule1, Raje
esh M Chomal1, Deepak R Patil2 and Yog
gesh S.
2
Shouch
he
1
Departtment of Microbiiology, Modern College, Shivajinnagar, Pune-4111005
2
National Centre for Cell Science, Pune-4411007.
Obessity is defined as
a abnormal fat accumulation thhat imposes a great
g
health
risk. The synddrome is just no
ot limited to thee western countrries, it has spraw
wled many
developing coountries and India is no exceptionn. Recently, an aalarming rate of prevelance
of abnormal aand visceral obeesity was reporteed from one of tthe metro cities.. A genetic
reason could not explain thiis obesity epideemic. Recent disscoveries have revealed a
strong associiation between gut microbiotaa and obesity. Although phyylum level
perturbations were reported, a keen hunt for an exclusively responsible bactterium is a
mplement these effforts, we initiatted screening gut microbial
hot topic of reesearch. To com
community inn a rat model of obesity. In this study, we have constructed andd screened,
16s rRNA geene based library
y for control annd obese wistarr rats. Approxim
mately, 200
clones were ssequenced for each
e
of the fouur animals in eaach group. Bioiinformatics
analysis confi
firmed that the ratio of Bacterooidetes to Firmiicutes is higherr in gut of
control rats than diet induced obese rats.
Published on 25tth February 2011
Page 34
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP330
Production of Bacteriial Cellulose by Acetobactter Xylinum (N
NCIM
2526) Using Fruit Waste and Checking
C
Forr Its Applicattion
Manasi karmark
kar, Poorva Dabir and Meg
ghana kukarni
manasi.mk
kar@gmail.com, poorva79@gm
mail.com
Department of Microbiologyy, Fergusson colllege Pune
The ppopulation increease necessitatess search for new
w biopolymers. Acetobacter
A
xylinum is onne of the widely
y studied microoorganism for thhe production of
o bacterial
cellulose(BC), Although plan
nt are major producers of celluloose, its degradattion results
ucts such as liggnin which doess not occur in caase of BC.
in release of hharmful byprodu
Nowadays, baacterial cellulosee finds wide appplications in paaper making, gaarment and
health care inndustries. Althou
ugh production of
o BC by A. xyllinum is known it is costly
which limits iits application on
o large scale. Inn present study economical prooduction of
BC using fruuit waste insteaad of synthetic media has beeen studied alonng with its
application ffor medical pu
urpose. Carbonn and Nitrogenn sources werre initially
standardized iin synthetic med
dium (Hesrin & schramm) for pproduction of BC.
B Results
indicate maxim
mum production
n of BC obtainedd using Fructosee (0.5%) and Yeeast extract
(0.5%). Fruit wastes (mashed
d banana peel annd mix fruit waaste) was substituuted in the
gen source for maximum
m
produuction of biomasss and BC.
medium as caarbon and nitrog
Cellulose obtaained was quanttified on dry weeight basis as weell as by specific chemical
method. Mix fruit waste was found to be moore suitable for tthe production of
o BC than
w coated
the banana peel. To prove its application in thhe health industryy, obtained BC was
on the cotton gauze and checcked with respecct to water absoorption & verticcal wicking
against the conntrol.
Published on 25tth February 2011
Page 35
State Level Conference on
Progressivve Education society’s
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
Miicrobiology in 211st Century
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP31
Aureobaasidium Pullu
ulans Grown on Various F
Feedstocks foor
Comparative
C
Studies
Meenakshi Dhar,
D
Nasim Kherad,
K
Vinay
y Rale
Deppartment Of Micrrobiology, Ferguusson College, P
Pune 411004
Aureobasidium pullu
ulans, a yeast-likke fungus has bbeen well-studieed and
duce pullulan, a polysaccharide of multiple usess. This
explored for itts ability to prod
organism alsoo produces differrent types of enzzymes which haave different acttivities
and along wiith the pullulan
n which is extraacellular; it alsoo produces the black
pigments callled melanin. Th
his organism annd its close relative, Aureobassidium
mausonii, hass been studied in
n our laboratoryy for quite som
me time and one of us
(Vinay Rale) hhas exhaustive research
r
experiennce with the orgganism. We undeertook
to study Aureeobasidium pullu
ulans with the sole
s
objective of growth and prroduct
formation on molasses and sp
pent wash at shaake flask and at batch-scale ferm
menter
mance (growth and product foormation) in staandard
level to comppare its perform
medium. The amount of biomass as well ass pullulan produuction is quite high
h
in
c
with shake
s
flask, whhen the fermenntation
batch- scale fermenter in compare
f
of this orgganism viz., agittation,
parameters waas optimized according to the favor
temperature, ppH, etc. . Also, the morphologyy change of the organism is obsserved
from shake flask to fermeenter. The seccondary objectivve was to maanifest
OD and COD values
v
of theses substrates, viz.., beet
concomitant rreductions in BO
molasses and spent wash from
m brewery industtry. The initial B
BOD and COD for
f the
h is 24000 mg/liit and 215000 m
mg/lit respectivelly and
spent wash is obtained which
o
which is 280000 mg/llit and
initial BOD aand COD for beeet molasses is obtained
2100000 mg/lit respectively. The results off this study of one so far are quite
m which producees less
interesting. Ouur last objective was to try obtainning an organism
or no pigmennts which itselff helps better result in extraceellular polysacchharide
production ass well as BOD and COD reduuction. This is ddone by exposinng the
organism undeer UV light for 20
2 minutes.
Published on 25tth February 2011
Page 36
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP332
Antimiccrobial Effectt of Bombax C
Ceiba
*Mo
onika More, *Gauri
*
Jadhav
v
*Sinhgad Colllege of Science, Ambegaon (Bk)), Pune 41.
Bombbax ceiba belong
gs to the family Bombacaceae. It found in tropiical region.
It is a large siized, tall, decidu
uous tree, whichh has been founnd to have manyy medicinal
uses. All parts of the tree succh as stem, leavves, root, flowerrs, and thorns arre used for
medicinal purrpose. It can be used in treatmeent of diabetes, kkidney stone, coonstipation,
asthama, foodd poisoning, paralysis, haemoptyysis of pulmonaary tuberculosis,, influenza,
leucorrhoea, m
menorrhagia, accne, bleeding piles,
p
diarrhoea,, dysentery, skiin disease,
dental caries, tooth ache etcc. Present studyy is concentrateed on extractingg bioactive
compounds frrom stem and leaves
l
using sollvents such as diethyl ether, chloroform,
c
methanol andd water. Effectss of these extraacts were furthher studied on pathogenic
organisms likke Proteus, Pseeudomonas, Stapphylococcus auureus, E. coli, Salmonella
S
typhi, and Caandida spp. Ressults will pave the way towardds better undersstanding of
antimicrobial activity of plantt extracts as welll as its purificattion. Purified prroducts can
be studied furrther for drug deesigning and plannt tissue culturee techniques for large scale
production of bioactive compo
ounds.
Published on 25tth February 2011
Page 37
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP333
Produ
uction and Pu
urification off Extracellulaar RNases froom
Streptom
myces venezuaalae (NCIM 22215)
Moksh
hada Godbole
e1, Neelima Ku
ulkarni1 and S
S. S. Deshmu
ukh2
1
Modern Co
ollege, Ganeshkhhind Road, Punee 411053.
2
Natiional Chemical Laboratory,
L
Punne
Among the five Sttreptomyces culltures from NC
CIM (National Centre for
N
Chemiccal Laboratory, Pune) screened for RNase
Industrial Miccro-organisms, National
production inn MGYP (Mallt extract, Gluucose, Yeast exxtract, Peptonee) medium
Streptomyces venezualae (NC
CIM-2215) show
wed maximum R
RNase activity (270
(
U/ml)
s
In optim
mization studiess effect of
and was selected for furtherr optimization studies.
o RNase production was studieed and the MGY
YP medium
different mediia components on
was modifiedd to PG (Pepton
ne, Glucose) medium which is simple and costt effective.
Effect of diffeerent organic and
d inorganic nitrogen sources on R
RNase productioon was also
studied and P
Peptone was fo
ound to be thee most suitable organic nitroggen source.
Inorganic nitrrogen sources reesulted in very poor RNase acctivity. In PG medium
m
the
RNase activityy increased by 4.8
4 times (270 U/ml
U
to 1302 U
U/ml) where as thhe specific
activity increaased by 6.7 tim
mes (1986U/mg to 13285U/mg). Hence this meedium was
selected for R
RNase production
n. The maximum
m extracellular R
RNase activity annd specific
activity was detected at 72 hours and maaximum intracelllular RNase acctivity was
a
was also observed but it was very less ass compared
detected at 96 hours. DNase activity
to RNase acttivity. Streptomyyces venezualaee produces non specific nucleaase. RNase
activity does not require meetal ions wheree as DNase acttivity requires Mn
M 2+ ions.
s
that heaat treatment is nnot a suitable method
m
for
Purification oof the enzyme showed
purification siince it resulted in
n loss of RNase activity.
a
Ammonnium sulphate prrecipitation
on. Ion exchangee chromatographhy was used in next
n step of
was the first step of purificatio
purification annd enzyme was loaded
l
on DEAE
E cellulose (anioon exchanger) coolumn. The
enzyme was eeluted unbound and
a other impuriities were removved from the enzzyme since
they bound too the column. Th
he enzyme was then
t
loaded on F
FPLC gel filtratiion column
(Superose-12)) and the final recovery was 10%. The speciffic activity of thhe enzyme
increased 2.3 ffold at this step of
o purification.
Published on 25tth February 2011
Page 38
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP334
Isolation S
Screening fo
or Penicillin-V
VAcylase Prroducing Orgganism,
Optimizattion of Cultu
ure Condition
ns, Effect of Media Constituents
on Producction of Enzyyme from B. Cereus
1
Neha Naik1, Avinash Sunder
S
, Laxmi Nair1, Vaiidehi Dande1 & A.V.
Pundle2
1
Modern collegee of Arts, Sciencee & Commerce P
Pune 411 007,
2
Natiional Chemical Laboratory,
L
Punne
mportant enzyme. Penicillin
Peniccillin-V acylase (PVA) is a pharrmaceutically im
acylases beloong to N-termin
nal nucleophile (Ntn)-hydrolasee super family, Penicillin
olase enzymes that
t
cleave peniicillin’s at the amide
a
bond
acylase proteiins , amidohydro
connecting thee side chain to th
heir B-lactam nuucleus, releasingg 6- aminopenicilllianic acid
( 6-APA) corrresponding sidee chain. 6- APA
A is the building block of sem
mi synthetic
penicillin’s .Penicillin acylasee acts as scavengger enzyme for pphenylacylated compounds.
o
was ddone from soil sample of
Isolation of 110 morphologiccally different organisms
Hindustan A
Antibiotic pu
une, Screeningg for penicillin acylase- producing
microorganism
ms was carried out
o by plate assayy method. Out oof which 1 isolaate showed
Penicillin–V acylase activitty. Activity waas checked by sensitivity of 6-APA to
serratia marccescens, compaaratively resistant to penicillinn. The morpholoogical and
physiologicall characterizatiion of the PVA-producing
P
Gram positivve isolate
ATUAVP18446 was performeed by a private institute,
i
using 16srRNA sequeencing and
FAME analyssis, isolate was identified as B.
B cereus. The 116S rRNA genee sequence
of ATUAVP 1846 was comp
pared with all otther known 16S
S rRNA gene seqquences of
bacillus spp.,, and a phylog
genetic tree waas constructed using Neighbour joining
method (CLU
USTAL X) wiith the related taxa. This is the first repoort on the
production off PVA by B.cerreus. Fermentattion parameterss such as Optim
mization of
Cultural Connditions and Effect
E
of Meddia Supplemennts for Maxim
mum PVA
production w
were studied in
n batch fermeentation under shake flask conditions.
c
Optimum inooculum size was found to be 10%.
1
Maximum
m enzyme produuction was
observed at 30°C and pH 7.0
0, after an incubbation of 24 hrs,, and optimum dispensing
d
m Erlenmeyer flask.
f
Out of aall used ‘C’ souurces used
volume was 75ml in 250 ml
(2.0%) max. PVA productiion is achievedd in the case of glycerol folllowed by
glucose folloowed by sucrose, and Out of all used ‘N’ soources used (0..3%) max.
PVA productiion is achieved in the case of ammonium
a
phossphate.
Published on 25tth February 2011
Page 39
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP335
Characcterization of Plasmid Enccoded Resistaance To β-Laactam
Antibioticcs of Acinetob
bacter Baumaannii AIIMS
S7, A Clinicall Isolate
Neha S. Vora, Shradh
ha B. Nadhe, Riddhi
R
J. Shah and Karishm
ma R.
*
Parde
esi
karishma@unnipune.ac.in
Departmeent of Microbioloogy, University oof Pune
Acineetobacter bauma
annii is an oppportunistic nosoccomial multidruug resistant
pathogen. A cclinical isolate from
f
All India Institute
I
of Meddical Sciences, New
N
Delhi,
AIIMS7 is muultidrug resistantt showing high resistance
r
to β-llactam antibioticcs. AIIMS7
consists of ffour plasmids, one
o high molecular weight (577 kb), one mid molecular
weight (20 kb) and two low molecular
m
weightt (<10 kb each).The objective off this study
was phenotyppic and biocheemical characterrization of the HMW plasmidd encoded
resistance to β-lactam antibio
otics. A transformant Acinetobaacter baylyi AD
DP1 (T17),
harbouring thee high moleculaar weight plasmiid was obtained by natural transsformation.
Phenotypic chharacterization of β-lactamases was
w performed bby using various disc assay
methods. In biiochemical characterization mollecular weight off β-lactamase waas detected
by SDS PAGE
E followed by acctivity staining with
w nitrocefin. P
Phenotypic charaacterization
demonstrated presence of carrbapenemase andd AmpC type off β-lactamase inn AIIMS7.
i the wild
Activity staining showed thatt of the two typpes of beta lactaamases present in
AIIMS 7 (19.31k
kD and 39.44kD
D), the transform
mant T17 showed only one
type isolate A
of the beta lactamase (39.4
44kD) which appears
a
to be pplasmid encodeed. Further
mase gene/s preseent on this highh molecular
molecular chaaracterisation off the beta lactam
weight plasmiid is under study
y.
Key Woords
Acinetobacter, Multidrug Resisstant, Plasmid, β
β-lactamase.
Published on 25tth February 2011
Page 40
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP336
Optimizzation of Gro
owth Parametters for Ethaanol Productiion by
Yeassts
Neelima Kulkarni*, Shreyas Kum
mbhare, Pandu
urang Lahare
e and
Hussain Kho
okhawala
Modern College of Arts, Science and Commerce, Ganeshhkhind, Pune 411053.
m different sugar containing foodd materials,
34 yeeast cultures werre isolated from
fruits, fermennted foods, sugaar syrups and agricultural wasstes using Gluccose Yeast
extract Peptonne (GYP) mediu
um. All the isolaates were screenned for ethanol production
and best ethannol producing cultures
c
BG-1, SCJ-3
S
and PG-22 were selected for further
single parameetric studies. Efffect of differennt carbon sourcces, nitrogen soources, pH,
temperature aand fermentation
n time on ethannol production was studied. Etthanol was
produced maxximally using 20
0 g/L sucrose ass carbon source , 10 g/L coconnut meal as
nitrogen source at pH-4, tem
mperature 30 °C
C, inoculum sizze 5 % (v/v) inn 58 hours
ours for SCJ-3 with
w ethanol conncentration of 7.77 g/L and
incubation forr BG-1 and 24 ho
10.659 g/L respectively as esttimated by Gas chromatographyy. The total carbbon content
medium for SCJ-3 was estimated and the yieldd of ethanol prooduced was
of optimized m
10.659 g/L oof medium conssumed. This givves 92.64 % effficiency of ethaanol yield.
Ethanol was pproduced maxim
mally for culture PG-2 using 20 gg/L fructose as the
t Carbon
source, 10 g//L urea as nitrogen source, pH-5, temperatture 37 °C andd 48 hours
fermentation time. During optimization studdies ethanol conncentration wass increased
L to 11.2 g/L wh
hen estimated byy CAN method aand 2.242 g/L too 5.180 g/L
from 6.12 g/L
as estimated bby Gas chromattography. The thhree best Ethanool producing yeaast isolates
PG-2, SCJ-3 aand BG-1 were characterized byy morphology, bbiochemical andd molecular
characterizatioon and identiffied as Kodam
maea ohmeri, Candida interm
media and
Torulaspora ddelbreucki respecctively.
Published on 25tth February 2011
Page 41
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP337
Compaarative in-vitrro Antimicroobial Activityy of the Extraact of
Gloriossa Superba ag
gainst Laboraatory and An
ntibiotic Resisstant
Clinical Isolates of Common Path
hogens
Nikita L.
L Lad and Ra
ajendra G. Choure
Deppartment of Miccrobiology, Instittute of Science, M
Mumbai 400032
Gloriiosa superba (faamily: Liliaceae)) is widely usedd as a medicinall plant, and
the alkaloids ffrom the plant (C
Colchicine and Gloriosine)
G
are bbeing used in thee treatment
of gout and rrheumatism. The increasing preevalence of multidrug resistantt strains of
micro organissms has stimulaated the exploraation of alternatte antimicrobial regimens.
Thus, taking iinto account thee medicinal impoortance of Gloriiosa superba Linnn., in this
respect, an atttempt was mad
de in the current study to invvestigate the anntimicrobial
potential of thhis plant. Extracct of dried leavees and tubers waas extracted in ethanol
e
and
methanol and their antimicrob
bial activity wass determined aagainst standard cultures of
occus aureus, Salmonella
S
typhhi, Klebsiella pneumonia,
p
Escherichia ccoli, Staphyloco
Pseudomonas aeruginosa, Prroteus vulgaris, Bacillus sp .andd 8 cephalosporiin resistant
clinical isolattes of Eschericchia coli (3 sttrains), Proteuss mirabilis, Enterobacter,
Klebsiella, Psseudomonas and
d Acinetobacterr. The extract aalso showed anntimicrobial
activity againnst cephalosporin resistant orgaanisms. The objject of this stuudy was to
formulate a nnew, cost effectiive antimicrobiaal combination for drug-resistannt diseases
based on syneergistic activity of
o cefotaxime wiith methanolic exxtract of Gloriossa superba.
Methanolic exxtract was found to act synergiistically with thhe cephalosporinn antibiotic
and inhibited ggrowth of the ceephalosporin resiistant organisms in presence of cefotaxime.
c
The highest synergism rate was attained against
a
Proteus mirabilis. Phyytochemical
screening of eextract showed presence of alkkaloids, flavonoiids and steroids.. Thus, the
current investiigation leads to fresh sources off new antimicrobbial regimes in future.
f
This
is the first repport in vitro wh
hich revealed syynergistic inhibiition of antibiotiic resistant
organism by tthe extracts of medicinally
m
impportant ornamenttal plant Gloriossa superba
(Kalhari).
Published on 25tth February 2011
Page 42
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP338
Exttracellular RN
Nase producttion and puriification from
m
Strep
eptomyces sp. (NCIM 20811)
Niruta Y. K
Killedar1*, An
nupama Sonaw
wane1 and Su
umedh S.Deshmukh2
1
Modern colllege, Ganeshkhiind, Pune 4110553. 2National Chhemical Laboratoory, Pune
omyces culture from NCIM ( National Colllection of
Among five Strepto
S
sp.(NCIM 2081) showed maximuum RNase
Industrial Miccroorganisms) Streptomyces
activity (257U
U/ml) during scrreening in MGY
YP(Malt extract 0.5%, Glucose 1%, Yeast
extract 0.3%, Peptone 0.5%) medium so selected
s
for opttimization studiees. During
M extract
optimization sstudies first effect of various meedia componentss was studied. Malt
and Yeast extrract when omitteed from MGYP medium
m
higher aactivity was obtaained. This
was useful inn reducing cost of
o the medium. The organism could grow in simple
s
BG
(Beef extract 0.8%, Glucose 1%)
1 medium insstead of MGYP medium. Effect of organic
and inorganicc nitrogen sourcces showed that organic nitrogeen sources prodduced more
RNase activitty than inorganiic nitrogen sourrces. Beef extraact was the moost suitable
nitrogen sourcce for RNase pro
oduction. Effect of initial pH on RNase productiion showed
maximum RN
Nase activity at pH
p 6.0 in BG medium.
m
Optimizzation studies reesulted in 2
fold increase in RNase activity and 6.6 foldd increase in sppecific activity. Production
CIM 2081) cultuure showed that in BG medium maximum
profile of Strpptomyces sp.(NC
extracellular R
RNase was prod
duced in 72 hrss. and maximum
m intracellular RNase
R
was
produced in 996 hrs. with 76%
% sugar utilized within
w
72-96 hrss. Streptomyces sp. (NCIM
2081) producced nonspecific nuclease produucing DNase atiivity also. RNaase activity
could be deteected in absencee of metal ions, but Mn+2 ion ffor DNase. Puriification of
enzyme show
wed that heat trreatment and Ammonium
A
sulphhate precipitatioon are not
suitable methhods which resulted in veryy poor recoveryy. During ion exchange
chromatographhy the enzyme came
c
unbound when
w
loaded on D
DEAE-cellulosee column at
pH 7.0. Enzym
me bound to CM
M-cellulose coluumn at pH 5.0 aand eluted with buffer and
0.5M salt resuulting in 3 fold purification andd 16.6% recoveryy. CM eluted ennzyme was
loaded on FPL
LC gel filtration column (Superoose 12) resulting in 8.9% recoverry.
Published on 25tth February 2011
Page 43
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP339
Isolatiion and Characterizattion of Oxallate Degrad
ding
Organ
nism
P.S, Pa
atil , Patil Sha
am A , Bedse,,Tanuja D, Ja
adhav Sandip
pR.
K.T. H. M. Colllege, Nashik
Kidnney/ urinary tract stone disease is a major healtth problem throughout the
world. Most oof the stone are composed
c
of oxaalate or calcium
m oxalate. Oxalicc acid or its
salt found in variety of food
d. Increased abssorption of oxallate mayoccure after food
containing eleevated amount of
o oxalate is takken. Humans laack the enzyme needed to
metabolize dietary oxalate. Oxalate
O
in humanns can be eliminnated through exxcretion in
urine, formingg insoluble calciu
um oxalate and excretion
e
in faecces and oxalate degradation
d
by gastrointestinal tract miccroorganism etcc. Oxalate degrrading facultativve aerobic
bacteria were isolated from different sources. Organisms weree characterized on
o the basis
mical observatioons. Among these organism onee organism
of morphologgical and biochem
belongs to gennus- Stomatococccus . This organnism is further ccharacterized forr utilization
of different ooxalate sources. Oxaltate degraadation product, formate was detected
d
by
FTIR analysis, which suggeest the productioon of oxalate ddegrading enzym
me oxalylcoAtransferase by the organissm. The amount of oxalate degraadation was deteermined by
nO4. The degradation rate of oxaalate by the isolaate is found
Titrimetric analysis with KMn
to be around 440%.
Published on 25tth February 2011
Page 44
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP113
Comparaative Study on
o The Produ
uction of Prod
digiosin by Seerratia
marcesscens Using Various
V
Crud
de Fatty Acid
d Sources and
d Its
Applicaations
Pankaj .S.
. Picha & Sh
heetal .P. Parrdeshi
pankaj.picha@
@gmail.com
Departmeent of Microbiollogy, Modern Coollege of Arts, Sccience and Comm
merce,
Shivajiinagar, Pune-4111005, Maharashhtra.
d by Serratia maarcescens is a prromising drug owing
o
to its
Prodiigiosin produced
reported charaacteristics of hav
ving antifungal, immunosuppresssive, antiprolifeerative and
anticancer acttivity. From an industrial
i
point of view the neccessity to obtainn a suitable
medium to sim
multaneously en
nhance the growtth of Serratia marcescens and thhe pigment
production waas the aim of thiis work. Pigmennt producing orgganisms were isoolated from
air and rhizosphere and screened for their abiility to produce pigment. One issolate, PP1
was selected ffor further studiees. It was biocheemically charactterized and identtified to be
Serratia marccescens by API kit. Productionn was optimizedd with respect to
t different
environmentall parameters usiing crude and pure
p
carbon sourrces. Extraction procedure
and purificatioon protocol was also modified annd developed too recover pigmennt. Medium
supplemented with fatty acid
d containing soource were founnd to give betteer yield of
pigment than defined mediaa. Crude carbonn sources reducce the cost of production
h pure carbon souurce and the piggment thus obtaiined can be
medium whenn compared with
used as coloraants in textile and
d paint formulatiions and also forr therapeutic appplications.
Key Woords
Prod
digiosin, Serratiaa, Pigment, Painnt.
Published on 25tth February 2011
Page 45
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP440
O
Oligotyping of
o Antibiotic Resistance G
Genes from
Clinicall Isolates of Acinetobacter
A
r Spp.
Poojari Pritika, Ratnak
karan Neena,, Mehta Khyatti, Shirode Ab
bhinav,
Pardesi Ka
arishma*
karishma@unnipune.ac.in
D
Department of Miicrobiology, Uniiversity of Pune, Pune 411 007
Acineetobacter spp. has
h emerged as important
i
nosocoomial pathogenss causing a
serious therappeutic failure. Raapid genotypingg methods for the identification and typing
of these organnisms have allow
wed a better apprreciation of the epidemiology annd survival
of these organnisms in the hosp
pital environmennt. In the presentt study, we charaacterize the
diversity of anntibiotic resistan
nce in twenty-siix isolates of Accinetobacter sppp. obtained
from various hospitals in In
ndia, which shoowed multidrug resistance by phenotypic
p
T genes for thee drug resistancee are either
assays and alsso harbored multtiple plasmids. The
chromosomal or plasmid enccoded. Genomicc DNA was isollated using Sigm
ma-Aldrich
was done using 30
3 pairs of
GenElute Baccterial Genomic DNA kit. PCR amplification w
primers speciffic for differentt classes of ß-lacctamases (Classs A, Class B, Class C and
Class D), aminoglycosides an
nd quinolones. Out
O of twenty-sixx isolates characcterized, all
n with more thann one primer. Maaximum isolatess had genes
showed positive amplification
owed by Class A,
A Class D andd Class B. The AmpC
A
and
for Class C ßß-lactamase follo
ADC type of ßß-lactamase wass found to be moore prevalent. Siimilarly aph-A6 was found
to be more preevalent among aminoglycosides
a
and gyrA, parC
C, QnrA among quinolones.
q
The resistancee of Acinetobactter to quinolones is of prime im
mportance since quinolones
are highly useed as a treatment now-a-days. If the genes for quinolone resistance are
present on plasmids, it willl lead to transfer of these geenes to other susceptible
b important inn epidemiologicaal point of
organisms. Hence this study will prove to be
view.
Key Woords
A
Acinetobacter, ß--lactamases, Am
minoglycosides annd Quinolones
Published on 25tth February 2011
Page 46
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP441
Optimiization of Am
mylase, Produ
uction by Fun
ngi Isolated from
f
Rhizospheere Soil
Pradnya N. Nadivkar and
d Sheetal. P. Pardeshi
Departtment of Microbiiology, Modern College,
C
Shivajinnagar, Pune 4111005
Four different Amylaase producing organisms were iisolated on potatto dextrose
o Botanical garrden of Modernn College of AC
CS, Shivaji
agar from rhiizosphere soil of
Nagar, Pune 005, after primary
y screening on sttarch agar plate. Two fungi werre taken for
further study (F1 & F2) whicch were showinng zone of clearrance on starch agar plate.
Three differennt media viz. sabouraud,
s
Amyylase specific m
media 1, Amylase specific
media 2 were checked for theeir ability to suppport maximum aamylase producttion, out of
medium sabouraaud showed maaximum producttion of amylasee. Physical
these three m
parameter likee pH and temperrature were optiimized using sabbouraud. Fungall strains F1
and F2 showeed optimum production of amyllase at pH 7 andd 6, temperaturee 280C and
320C respectivvely. Time coursse of amylase prroduction was allso determined for
f both the
isolates.
Published on 25tth February 2011
Page 47
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP442
Redu
uction of heav
vy metals from
m domestic ssewage by usiing
Microorgganisms
P
Prajakta Bhag
gwat, Priya Bendre
B
and Vrrushali Patil
bendrepriya61@
@gmail.com
Department of
o Microbiology, Fergusson Colllege, Pune
Rapidd population grrowth, urbanizattion has increassed the need foor reuse of
wastewater inn agriculture. Waastewater poses certain environnmental and heallth risks to
consumers. Sludge is compo
osed of by-prodducts collected at different stages of the
wastewater trreatment processs. It contains booth compounds of agricultural value and
pollutants, whhich usually conssist of heavy mettals, organic polllutants and pathogens. The
characteristicss of sludge depeend on the origiinal pollution looad of the wasteewater and
also on the effficiency of its trreatment, follow
wed by the treatm
ment of sludge. The World
health organizzation (WHO) esstimates that 80%
% of all chronic disorders can bee attributed
to heavy metaal contamination
n, as it producees free radicals. Presence of heaavy metals
reduces the eefficacy of med
dical treatments by up to 60%. Heavy metal toxicity is
directly or inddirectly linked to
o many complicaations such as heeadaches, anger etc. and to
disorders suchh as arthritis, assthma, chronic fatigue syndrom
me, diabetes, fibbromyalgia,
heart disease, arterial sclerosiis, multiple scleerosis, Parkinsonn's disease, ulceers etc. and
une system. It haas been reportedd that the conceentration of
depletion of thhe body's immu
heavy metals can be reduceed by using baccteria and funggi such as Aspeergillus for
bioremediationn of domestic sludge to reducce its toxicity. Hence, bioremeediation of
sludge to redduce heavy mettals content wass studied. Sludgge was neutral in nature,
contained 4.21- 6.43 mg/l co
opper and 6.0-8.15mg/l zinc. A
Among the twennty isolates
m sludge, four iso
olates could rem
move up to 54.766% copper and 2.11%
2
zinc
obtained from
in 24 hours. Studies on reducttion of copper frrom sludge were carried out withh respect to
environmentall factors such ass temperature, pH, carbon sourcce and nitrogen source and
incubation tim
me. One of the isolates, R, showeed optimum reduuction of copper up to 71%
at ambient teemperature (28±2oc), pH 7, 1%
% glucose conncentration, 0.1%
% peptone
concentration and incubation
n for 28 hours, proving to be uuseful in the reeduction of
copper from ddomestic sludge.
Published on 25tth February 2011
Page 48
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP443
Isolation and Charracterization
n of Indole Accetic Acid (IA
AA)
phere of Gram
m (Cicer Arieentinum)
Producingg Organism frrom Rhizosp
Priya
anka Mane, Smita
S
Bhuyan
n and Dr. Shillpa Mujumdar*
ment of Microbio
ology, Modern College,
C
Shivajinaagar, Pune – 05..
Departm
ollected from rhhizosphere of graam was mixed in 10 ml of
1 g oof soil sample co
sterile saline. Serial dilutions of the sample were prepared aand 0.1 ml of thhis dilution
was spread onn sterile Luria –B
Bertanni agar plaates. After incubbation at 30⁰C foor 24 hrs ,8
colonies werre selected an
nd screened foor preliminary characteristics by their
morphological features ,Gram
m character ,motiility, oxidase tesst, catalase test and
a capsule
Media used wass LB broth
staining and tthen for IAA prroduction by Sallkovaski’s test. M
without tryptoophan. Red colo
our formation was
w considered ppositive evidencce for IAA
production. A
All showed IAA production. Outt of 8, P2 and P
P4 isolates gave maximum
IAA productioon than others. Cultures
C
were prreserved on LB agar slants for further
f
use.
The effect off time course an
nd carbon and nitrogen
n
sourcess on IAA produuction was
P2 and P4 isolattes by Salkovaski’s test. Mediaa used was LB broth with
checked by P
tryptophan (1 mg/ml). Unino
oculated LB brooth with tryptopphan was kept as control.
was checked at 540 nm. Maxim
mum IAA was produced at 722 hours of
Absorbance w
incubation byy replacing carbo
on source (yeastt extract) from L
LB broth with sucrose and
by using trypptone as nitrogeen source. Effect of temperatuure and pH was checked.
Maximum IAA production was
w seen at 37°C
C for P2 isolate and 32°C for P44 isolate at
pH 8. P2 wass identified as M.
M lacunata and P4 as unidentifi
fied organism byy mini API
test.
Published on 25tth February 2011
Page 49
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP444
Wound
d Healing Prooperties of hooney
Purnim
ma Ranawat and Jyoti Mantri
Department of
o Microbiology, Sophia Collegee, Mumbai.
Healiing of infected and chronic wounds
w
is a com
mmon challengee faced by
doctors; this pproblem has receently escalated due
d to the persisttence of antibiottic resistant
bacteria. Hennce new approaaches for treatinng wounds are required. Therre is some
evidence that honey helps in accelerating
a
wouund healing and tthus should be loooked at as
an alternate fform of treatmeent. A study waas conducted to evaluate the anntibacterial
potency of coommon honeys found
f
in the marrket namely- Daabur, Pondaghat and Khadi
honey againstt S. aureus, P. aerugenosa, Strepptococci and Caandida albicans.. MIC with
the above honneys was carried out using agar dilution
d
method (range 5%-30% v/v) and it
was found thaat Khadi honey was
w most potentt and had the leaast amount of coontaminant
spores. All thee honeys were th
he most effectivve against pseudoomonas, knockinng it out at
10% v/v conccentration while it took a conceentration of at leeast 25% to knoock out the
rest. Candida persisted beyond
d 30% v/v conceentration of honney. A control MIC
M using a
t see if the
sugar solutionn with concentrattion of sugars cloose to that in honney was set up to
properties of hhoney were soleely due to the ossmotic effect off the sugars pressent. It was
found that all the four organissms mentioned survived
s
beyondd 30% concentraation of the
h
sugar
sugar solutionn indicating thaat honey has soome properties other than its high
content that aaccount for its lo
ower MIC’s. Sinnce honey was ffound to be mosst effective
against the labboratory strain of Pseudomonas aeruginosa, fourr antibiotic resistant strains
of the same oorganism were tested for theirr sensitivity to hhoney using aggar dilution
method. It waas observed thatt honey inhibitedd these strains aat a concentratioon of 15%.
The effect of hhoney and SSD ointment (a com
mmon antibiotic ccream used on wounds
w
and
infected burnss) on preformed biofilms of S. aureus and P. aerruginosa was evvaluated by
colorimetric m
measurement of biofilms using crystal
c
violet. It was seen that 50% diluted
honey was sllightly more efffective than SS
SD on biofilms of the above organisms.
Furthermore tto test the effect of honey onn wound healingg, angiogenesis by chorio
allantoic mem
mbrane assay was performed usinng fertilized Legghorn chicken egggs. It was
found that honney accelerated the
t rate of angioogenesis as comppared to a positiive control,
a negative conntrol and a salinee control. This suggests
s
that anggiogenesis may also
a be one
of the factors tthat make honey
y more effective in wound healinng.
Published on 25tth February 2011
Page 50
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP445
Optimiization and Characterizat
C
tion of Lipasee Production by a
Selected Bacillus sp. and
a Its Appliication
Rahu
ul Brid and Ra
ajendra Chou
ure
D
Department of Microbiology,
M
Thee Institute Of Sciience, Mumbai
The growing intereest in lipase production is related to thee potential
biotechnologiccal applications that the enzymee present and soo there is immennse need to
discover and ddevelop new com
mmercial microbbial sources for liipase productionn. With this
need of time the present work deals with liipase productionn Bacillus speciies isolated
w
the
from stagnantt fish runoff waater and its chaaracterization annd application wherein
isolate was issolated on Victtoria Blue Olivee Oil Agar wheere lipase produuction was
indicated by zone of hydrollysis of olive oil.
o Optimizationn of medium components
m chloride, olivee oil, potassium
m chloride as ann optimum
revealed glucose, ammonium
T (28⁰C ± 2⁰C)) as optimum tem
mperature with optimum pH off 7.0 under
source and RT
shaking condition for favoraable lipase prodduction. Producttion was carriedd out with
optimized parrameters and feermentation studdies revealed thhat 50 hours waas best for
recovery of culture medium
m for extractionn and purificattion of lipase. Cell free
ntrifugation wass further purifieed by ammoniuum sulfate
supernatant oobtained by cen
precipitation aand dialysis. Furrther, cell free suupernatant was uused as enzyme source for
characterizatioon of lipase. Opttimum enzyme activity
a
was obseerved at pH-7.0 and RT0C.
The enzyme w
was found stablle in wide rangee of pH and tem
mperature. Potassium ions
stimulated enzzyme activity up
p to 493.33%. 1%
1 iso- propyl aalcohol was fouund to be a
good organic solvent enhancin
ng the lipase acttivity up to 166.666%. Tween-80 was found
to be effectivee stimulator for lipase activity increasing it up to 173.33%. Thhe enzyme
exhibited goodd stability with bleach
b
i.e. Sodiuum Hypochloritee. Olive oil was found
f
to be
the best subsstrate, among those
t
tested. The
T
lipase activvity was foundd stable in
commercial ddetergent which
h was even more
m
enhancedd in presence of CaCl2.
Immobilizatioon of lipase was carried in differrent carriers by eentrapment and its specific
activity was sstudied where 2% agarose had the highest speccific activity. Thhe purified
lipase was fouund efficient in complete
c
removaal of olive oil froom cotton fabric along with
detergent. Alsso the enzyme was found to have
h
antagonistiic effect on beeef fat as it
degraded the fat completely. The lipase hydrrolyzed castor ooil more than 899.28% in 6
days.
Published on 25tth February 2011
Page 51
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP446
Laboratoryy Study on Biioremediation of Diesel O
Oil Contaminated Soil
from a Garage
G
Rakhi Tari and Shilpa
S
Sabnis
s
Depaartment of Micro
obiology, The Insstitute of Sciencee, Mumbai 4000332
Polluution caused by
y the release of petroleum hhydrocarbons has
h
always
threatened health and sustain
nability of the environment.
e
D
Diesel is one of the major
pollutants. Beeing water insolu
uble, diesel oil also
a shows low and slow biodeggradability.
Surfactants iincrease the aqueous
a
solubillity and consequently degraadation of
hydrocarbons.. However chem
mical surfactants are relatively toxxic, less biodegrradable and
show limitedd efficiency. On
O the whole biosurfactants are specific in action,
biodegradablee, less toxic, can
n be prepared easily
e
and show
w widespread appplicability.
Therefore in tthe present study
y an attempt waas made to isolatte a biosurfactannt producer
and asses its role in the bio
oremediation of diesel oil. On screening garagge samples
a it’s by produucts, six isolates were obtained and
a the best
contaminated with crude oil and
isolate was sselected on thee basis of its emulsification activity measurred by oil
emulsificationn assay. It was screened
s
for thee better emulsification activity in
i different
carbon sourcees (sucrose, gluccose and lactosee) using the sam
me oil emulsificaation assay.
The selected isolate was found
d to be Pseudom
monas which gavve maximum emuulsification
with glucose. The biosurfacttant was extractted from cell frree broth of Pseeudomonas
using Acetonne precipitation method and annalyzed for its protein and caarbohydrate
content usingg Folin Lowery
y and Phenol Sulphuric acid method respeectively. A
comparative aanalysis of purifiied biosurfactantt with chemical ssurfactant (Tweeen- 80) was
carried out. 661.53% biodegraadtion was found in presence oof purified biosuurfactant in
comparison w
with 55% for Tween- 80 whhereas only 42..85% biodegraddation was
observed using whole biosurfa
factant producer. The biosurfactaant was also founnd to bring
about greater reduction in thee surface tensionn in comparisonn with chemicall surfactant
(Tween- 80). Thus, this study
y revealed the effficacy of biosurrfactants over thhe synthetic
surfactant in bbioremediation.
Key Woords
B
Biosurfactant, Diiesel oil, Chemiccal Surfactant, B
Bioremediation
Published on 25tth February 2011
Page 52
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP447
Production
n of Gibberelllic Acid by Sooil Fungi
Ravindrra Baviskar, Sunny
S
Gaikwa
ad, Shilpa Mu
ujumdar and Smita
S
Bhuya
an*
@gmail.com
*smitabhuyan@
Departm
ment of Microbiology Modern colllege of Arts, Sciience and Comm
merce,
Shivajinagar Puune 411 005
Gibbberellin (GA) is a plant hormoone that regulatees growth and influences
C
its w
wide array of appplications,
various devellopmental proceesses in plants. Considering
production off gibberellin and
d its enhancemennt by sources likke fungi and baccteria is of
prime importaance and it maay also meet thee ever increasinng demand of gibberellin.
g
Present work focuses on prod
duction of gibberrellin by four different fungi isoolated from
paddy field saamples. Spectrosscopic analysis revealed
r
four prromising fungal gibberellin
producing cultures in Czapek Dox broth at 2990C for 7 days. O
Optimization of parameters
like pH and teemperature and id
dentification of the
t fungal culturres is in process..
Key Words
Giibberellin, hormoone, Fungi, Czappek Dox
Published on 25tth February 2011
Page 53
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP002
Prevalencce of Methiciillin Resistant Staphylococccus aureus in
n D. Y.
Patil Hospittal and Reseaarch Centre, K
Kolhapur
Reen
na A. Dighe an
nd Saral Ghos
sh
drmrsdighe@reddiffmail.com
Deppartment of Micrrobiology D. Y. Patil
P
Medical Coollege, Kolhapurr.
biquitous in naturre with about a dozen species occcurring as
Staphhylococci are ub
part of humann flora. The mostt virulent of the genus, Staphyloococcus aureus, is the most
common causse of bacterial infection. Afterr the discovery of penicillin inn 1940 the
mortality ratee from staphylo
ococcal infectioons dropped drramatically. How
wever, the
golden periodd was soon interrrupted by the apppearance of peenicillin resistantt S. aureus
strains. Severaal different classses of antibioticss were developedd in response. Methicillin
M
,
a semi synthhetic penicillin was introduceed in 1959. F
First Methicillinn resistant
Staphylococcuus aureus was reported in 1961 in UK. Sinnce then it becaame major
nosocomial paathogen worldw
wide, popularly designated
d
as MR
RSA. Methicillinn was later
replaced by a more stable drug Oxacilllin. Staphylocoocci spp. has a unique
i well known too develop resisttance to antibiottics rapidly
epidemiologiccal pattern and is
and thereforee very difficult to treat, especcially those conntracted in the hospitals.
Prevalence off multidrug resistant Staphylocoocci amongst patients, hospitall personnel
and environm
ment can be a cause
c
of outbreeak of infectionn in the hospitaal. Regular
surveillance iis important ass a preventive measure. Clinical samples reeceived in
Microbiology laboratory of Dr.
D D.Y.Patil hospital.
h
Kolhappur within a perriod of 12
aphylococci
months were studied for Meethicillin resistannt staphylococccus isolates. Stap
M
were also isollated from hospiital personnel annd environment aand studied for Methicillin
resistance. Ouut of 150 Meth
hicillin resistantt staphylococci isolates from the clinical
samples 106 (70.6%) were identified as Staaphylococcus auureus i.e. MRSA
A and 44 (
mongst the hosppital personnel 13.33% were MRSA
M
and
29.4%) were MR-CoNS. Am
MR-CoNS. From
m the air sam
mpling of the hhospital environnment 204
2.8% were M
staphylococci were isolated, out
o of which, 211.66% were MR
RSA and 6.94% were MRCoNS.
Key Woords
RSA, Clinical sam
mples, Hospital personnel,
p
Hospiital environmentt.
MR
Published on 25tth February 2011
Page 54
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP448
Production of Xantha
an Gum By Xanthomonas
X
Compestris Using
Ind
dustrial and Kitchen
K
Wasste as a Produ
uction Mediaa
Repe A. B., 1Dhutekar S. S., 2Dr. Ranje
ekar M. K.
1.
Lecturer inn Postgraduate Department
D
of Microbiology,
M
Daayanand Sciencee College,
Latuur
2.
Heaad, Department of
o Microbiology Dayanand Sciennce College, Lattur
Xanthhan gum is poly
ysaccharide used as food additivee, commonaly ussed as food
thickening ageent and stabilizeer. One of the most
m remarkable properties of xaanthan gum
is its ability too produce a larg
ge increase in viiscosity of a liquuid by adding a very small
quantity of guum. Xanthan gu
um helps to creeate pleasant texxture in many ice creams.
Xanthomonas compestris is th
he pathogen isoolated from the iinfected leaves of
o cabbage
ular polysacchariides when inocuulated in fermenttation broth
was able to prroduce extracellu
containing bannana peel extracct as sugar sourcce, cabbage extrract as nitrogen source and
other ingredieents. After ferm
mentation that can
c vary in tim
me one to four days. The
polymer mat be precipitated from the mediuum by the addition of alcohol,, dried and
w be readily sooluble in water.
milled to give a powder that will
Published on 25tth February 2011
Page 55
Progressivve Education society’s
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
Depaartment of Microb
biology
State Level Conference on
Miicrobiology in 211st Century
25th – 26th February
F
2011
Absttract Book
OP112
Synthesiis and Characcterization off ZnO Nanop
particles and Their
Applicatioon on Textiless as Antimicrrobial Agentss and UV Abssorbers.
Rutuja
a R. Phatate, Rebecca S. Thombre
T
and Sonal P. Bath
hija
Departmeent of Biotechnollogy, Modern Coollege of Arts, Sccience and Comm
merce,
Shivajinagar, Pune
P
411 005
The aapplication of naanoscale materiaals and structurees, usually rangiing from 1
to 100 nanom
meters is an emerging area of naanoscience and nnanotechnology. Synthesis
of nanoparticles is an area of constant interestt for its wide rannge of applications.
nthesized usingg simple chem
mical method and their
ZnO nanopaarticles were syn
ng UV spectrosccopy, Scanning eelectron microcoopy, and Xcharacterizatioon was done usin
ray diffractionn. The antimicro
obial activity of ZnO nanoparticcles was studied using agar
well diffusionn technique. An
A awareness of personal saanitation, contaact disease
transmission and personal protection
p
has led
l
to the deveelopment of anntimicrobial
textiles. Appplication of efffective antibacterial agent annd UV absorbbers- ZnO
nanoparticles to textiles for producing antim
microbial and U
UV absorbing teextiles is a
future aspect oof this project.
Key Woords
ZnO Nanoparrticles, Antimicrrobial agent, UV
V absorbers
Published on 25tth February 2011
Page 56
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP001
Candida G
Glabrata Infecction of the Nasal
N
Septum
m – An Unusu
ual Case
Repoort
Roma A. Chougale
C
neetiroma@ggmail.com
Lecturer in Deepartment of Microbiology., Dr .D. Y. Patil Meddical College andd Research
K
Institute, Kolhapur
Canddida glabrata, formerly
f
called Torulopsis glabbrata is small unicellular,
u
eukaryotic, buudding yeast, usu
ually round to ovval in shape. It m
multiplies by prooduction of
blastoconidia. It is the onlly species of genus Candidaa that does noot produce
pseudohyphaee. Candida glabrrata was considered as relativelly non-pathogennic and part
of normal florra of healthy ind
dividuals and rarrely causing serrious infections in
i humans.
However, following the wid
despread use off Immunosuppreessive therapy with
w
broad
spectrum antifungal therapy, the frequency of
o mucocutaneouus and systemicc infections
caused after ccolonization by Candida glabratta has significanntly increased inn older age
groups over the past few years.
y
While Caandida albicans remains the major
m
yeast
pathogen, seveeral other speciees like Candida tropicalis,
t
C.krussei and C.glabraata are now
known to ccause opportun
nistic infectionss in septicemiia, urinary traact, organ
transplantationn, meningitis, pneumonia
p
and haematologic m
malignancies. Thhe primary
concern for C
Candida glabratta is that it is less susceptiblee to Amphotereecin B and
Fluconazole aand can develop
p resistance to other Azoles. H
Hence these infeections are
difficult to treeat. For this reason, it is clinicaally important thhat a pure growth of yeast
from a deep - seated source (i.e
( fluid or tissuue) be fully specciated. Without speciation,
Physicians woould tend to use an Azole agent for treatment duue to the decreassed toxicity
of these druggs. Patients witth Candida glabrata should bbe immediately placed on
Amphoterecinn-B, since this is the only eff
ffective therapy for Azole-resisstant yeast
infection. Herre is presented
d the case repoort of a 58 yeaar old male paatient with
uncontrolled D
Diabetes mellitu
us, having an innfected nasal sepptum, diagnosedd clinically
and histopathoologically as “su
uspected mucorm
mycoses”. Microoscopy of infecteed tissue by
Gram stain annd Lactophenol cotton Blue mouunt of colonies revealed small 3-5
3 µ, oval
budding yeastt cells. Yeast collonies were seenn on Blood agar and Sabouraud’’s Dextrose
agar. The absence of pseudoh
hyphae and chlaamydospores onn Dalmau plate culture
c
and
appearance off dark pink to viiolet colonies onn CHROMagar C
Candida medium
m offered a
rapid and cosst- effective diaagnosis of Canddida glabrata. S
Screening for noon-albicans
Candida speccies should be performed on all
a clinical speccimen suspectinng mycotic
infections.
Key Woords
Candiida glabrata, CH
HROMagar Canddida Medium, Daalmau Plate cultuure
Published on 25tth February 2011
Page 57
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP449
Biological Synthesis of Silver Nanop
particles and
d Evaluation of
o Their
Antibacteriaal Activity
*Sabah Shaikh an
nd Shilpa Sabn
nis
D
Department of Microbiology,
M
thee Institute of Scieence, Mumbai.
The iintegration of naanomaterials witth biology is finnding wide applicability in
various areas of medical scien
nce. Silver has been
b
long recoggnized as havingg inhibitory
m
and induustrial processes. But silver
effect on varioous microorganisms present in medical
nanoparticles (SNPs), when sy
ynthesized by wet
w chemical reduuction method arre found to
be toxic, flam
mmable and not at
a all environmeent friendly. Theerefore in presennt study, an
attempt was m
made to formulate a cost effectivve and environm
ment friendly tecchnique for
green synthessis of SNPs, which
w
were synnthesized using extracts of Alllium cepa,
Azadirachta inndica and Carica
a papaya with AgNO
A
3 solutions in varying ratios (1:2, 1:4)
and their antibbacterial activity
y was studied ussing disk diffusioon method on issolates viz.,
Escherichia ccoli, Klebsiella pneumonia, Pseudomonas
P
aaeruginosa, Stapphlococcus
aureus and SSteptococcus pyo
ogenes obtainedd from bed sheeets of casualty ward
w
from
Prince Aly K
Khan Hospital. SNPs
S
(1:2) show
wing maximum antibacterial acttivity were
characterized by uv-vis spectrroscopy and scannning electron m
microscopy (SEM
M) and then
h and their antibbacterial activityy on the same issolates was
impregnated oonto cotton cloth
studied. The eeffect of consecutive washing of
o the coated dissks with distilledd water on
antibacterial pproperty was also investigated. The
T antibacteriall activity of the cotton bed
sheet coated w
with SNPs could be retained up to
t 3 washings. Siilver nitrate on itts own was
bactericidal too the isolates an
nd of all differeent plant extractts, Carica papayya showed
maximum anntibacterial activ
vity. These plannt extracts werre further foundd to show
synergistic baactericidal effect with AgNO3. This
T work demonnstrates the posssible use of
biologically synthesized SN
NPs incorporateed in cotton bbed sheets to minimize
nosocomial innfections.
Key Woords:
Silver naanoparticles, Anttibacterial, Plantt extracts, Escherrichia coli, Klebbsiella
pneumonia, P
Pseudomonas aeeruginosa, Staphhlococcus aureuss, Steptococcus pyogenes,
p
Allium cep
pa, Azadirachta indica, Carica ppapaya.
Published on 25tth February 2011
Page 58
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP550
Study of Ocular Microfflora, Antimicrobial and A
Antiadhesive Activity
of MPS
S Solutions on
n Ocular Isollates
*Sag
gar Vandra & Shilpa Sabnis
@gmail.com
sagarvandra@
Deppartment Of Miccrobiology, Instittute Of Science, M
Mumbai 4000322
p
to be useeful for visual coorrection but lysozyme and
Contact lenses have proved
ound to provide a good environm
ment for biofilm
m formation
albumin preseent in tears are fo
by many ocuular organisms. Therefore
T
attem
mpt was made too study the miccroflora on
various MPS against these isolates. A surveyy was carried ouut at 17 differennt opticians
mples were colleected from peopple between 122-49 years.
across Mumbbai and 50 sam
Various isolattes viz. Staphylo
ococcus aureus. Streptococcus m
mutans, Candidaa albicans,
Proteus vulgaaris, Klebsiella pneumoniae, Pseudomonas
P
sppp. were detecteed in these
samples. Variious multipurposse solution usedd for lens cleaninng were assesseed for their
antimicrobial activity was stud
died against thesse isolates using modified ISO 14729, Disc
diffusion andd Agar well diffusion methhod. Their peercentage inhibbition was
studied.Antiaddhesive activity of these isolatee was studied bby contact lens adherence,
coverslip adhherence method and by scanninng electron miccroscopy. The strength
s
of
adherence of aall isolates were found to be deccrease in presencce of MPS. Out of all MPS
solutions ReN
Nu and Compleete were found to show maxim
mum antibacteriaal activity.
Where Flexileens and ReNu showed
s
maximaal antiadhesive pproperty. The anntiadhesive
affectivity of tthese MPS was found to increasse when used in presence of vibbration type
cleaning devicce.
Published on 25tth February 2011
Page 59
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP551
Identificattion and Extrraction of Tob
bacco Mosaicc Virus (DNA
A) Using
Molecular Techniques
T
Samridd
dhi R. Sharma
a1 and Aparna
a Das2
1
Drr. D. Y. Patil Insttitute of Biotechnnology and Bioinnformatics, Punee
2
INDAM, Baangalore
Tobaacco mosaic viru
us is found in toobacco plants. IIn this project thhe infected
and healthy toobacco leaves are
a analysed by SDS-PAGE (S
Sodium Dodecyll SulphatePolyacrylamidde Gel Electroph
horesis). The tottal viral protein is separated usiing ELISA
(Enzyme Linkked Immunosorb
bant Assay), wheere the color chaange in ELISA plate
p
shows
the presence oof viral protein. The protein is electrophoresed and the proteinn bands are
transferred onnto Nitrocellulosse Membrane. This
T
gives the pposition of prottein of our
interest. Laterr the genomic DNA
D
is extractedd (Genomic DN
NA Extraction) by
b Agarose
Gel Electrophhoresis. Certain viruses
v
such as ToMV
T
are very eeasily isolated annd removed
and thus create a new hybrid plant
p
with healthhy factors.
Key Woords
ToMV, SDS
S-PAGE, ELISA
A, Genomic DNA
A, Nitocellulose Membrane, Agaarose Gel
Published on 25tth February 2011
Page 60
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP552
Phylogeenetic Analysiis of Insulin (Protein)
(
Usiing Bioinform
matics
Tools
Sandeep Gajbhiye
e1, Mr. Sunaka
ar2
1
Drr. D. Y. Patil Insttitute of Biotechnnology and Bioinnformatics, Punee
2
Hyderaabad
The project focusess on the phyloogenetic and sttructural study of protein
t
from
(insulin) in siix different speccies the query sequence of thesse species was taken
NCBI (Nationnal Centre of Bio
otechnology Infoormation). The sppecies such as Bos
B Taurus,
Mus muscullus, Gallus ga
allus, Drosophhila melanogasster, Rattus norvegious,
n
Caenorhabdittis are sequencced using the tools of bioinnformatics. The sequence
alignment waas done and ph
hylogenetic anallysis was perfoormed dendrogrram of the
protein sequennce was constuccted. the tools used
u
for the anallysis are blast, workbench,
w
pfam, embosss, cath, target p, hyperchem, hex, signal p. are used and is com
mpared the
species relatioon on the basis of insulin struccture. It can be used for the taaxonomical
studies of diffferent species.
Key Woords
Phylogeneticss - to show evolu
utionary relationsship, Mus muscuulus - house rat Bos
B Taurus
- cattle, Galllus gallus - roster, Caenorhabdittis - worm
Published on 25tth February 2011
Page 61
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
MPP
P53
Microbial Degradatio
on of Diamin
nodinitroetheene, a High Energy
E
Nitroexp
plosive
Sayali Ma
ahajani, Abhijit More, Seem
ma Sarnaik an
nd Pradnya Kanekar
K
Microbiall Sciences Divisiion, MACS-Aghaarkar Research IInstitute, Pune 411 004
Nitrooexplosives com
mprise a major component of nitro compoundds and are
highly energeetic materials. Use
U of nitroexpllosives is imporrtant for nation’’s security.
Environmentaally transformed
d products of these nitroexpplosives are harmful
h
to
environment and human beiings. Present stuudies include bbiodegradation of
o recently
developed innsensitive high energy nitroexxplosives 1,1-ddiamino-2,2-dinittroethylene
(FOX-7). It iis one of the im
mportant nitro explosive
e
of claass called as hiigh energy
insensitive exxplosive (IHE). It is synthesized by HEMRL P
Pune, by the treeatment of
acetamidinium
m chloride with diethylmalonatee to obtain 2-meethyl-pyrimidinee-4,6-dione
which on nitrration followed by hydrolysis gives
g
FOX-7. It is an aliphatic compound
with two nittro and two am
mino groups attached
a
to carbbon-carbon douuble bond.
Wastewater ggenerated during
g the productionn of FOX-7 is hhighly acidic annd contains
FOX-7 in highh amounts and itt appears to be allergic
a
to humann beings causingg symptoms
like methaem
moglobnaemia. Therefore biodegradation stuudy of FOX-77 and its
environmentallly transformed
d metabolites iss necessary. 133 bacterial isollates were
obtained by ssoil enrichment technique usingg the soil samplles collected froom FOX-7
production site. Out of these four
f
bacterial isoolates comprisingg 3 Actinomycettes and one
ould use FOX-7 as the sole sourrce of carbon annd nitrogen
isolate of Miccrococcus sp. co
when incorporrated in synthetiic medium at thhe concentration of 500 mg/l. Att the initial
concentration of FOX-7 as 400 mg/l in the synnthetic medium, the removal was enhanced
m
with 0.011% peptone. Theese isolates
from 20% to 440% after suppleementation of medium
were also able to grow in the wastewater geenerated during production of FOX-7
F
and
diation purpose.
thus can be ussed for bioremed
Published on 25tth February 2011
Page 62
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP110
Microbial Bioaugmentation for Treeatment of In
ndustrial Effluents in
Common Effluent
E
Treaatment Plant (CETP)
Snehal Barri, Nilesh Sonu
une, Amit Sin
nnarkar, S. S. Sarnaik, G. K.
K Wagh,
P. P. Kan
nekar
Microbial Sciences Divisio
on, MACS - Aghharkar Research Institute, Pune 411004
4
mical industries play an importtant role in devvelopment of anny country
Chem
however, theyy also contributee to environmenntal pollution particularly water pollution
due to large vvolume of efflueent generated duuring the producction and presence of toxic
chemicals eithher in the form of unused raw material or unreecovered finisheed product.
Effluent treattment plant thu
us becomes an unavoidable ccomponent of a chemical
industry. Sincce it is difficult to set up an efffluent treatment plant independdently for a
small scale unnit, existence of common effluennt treatment plannt (CETP) becomes useful
for cluster of such small scalee units. The wasstewater in CETP
P is usually heteerogeneous
ffluent using a single, pure
in nature. It is quite difficult to treat such tyype of mixed eff
water under studdy is from a CET
TP receiving efflluents from
microbial cultture. The wastew
chemical induustries producin
ng dyes and pigments
p
(e.g. D
Direct blue, Yellow
Y
FG,
Magenta, Crystal violet, Acid yellow, Fast garrnet GBC); pestticides (Temephhos, Ethion,
Hexaconazole) , pharmaceuticaals (Bitamethazzone valerate, Fluoxetine
Turbuphos, H
hydrochloridee, Cyclosporin),, petrochemicalls (spray oil, lliquid paraffin)) etc. The
wastewater is highly acidic in
n nature and darkk in appearance with appreciablee settelable
oad in terms of Chemical Oxyggen Demand (CO
OD) to the
matter. It conntains organic lo
extent of 8000 mg/L and am
mmoniacal nitroggen up to 900 m
mg/L. Bacterial consortium
c
C
and
was developedd using the bactteria isolated froom the soil in thhe premises of CETP,
bacterial consortium used at site.
s
The bacteriaal consortium deeveloped lowered the COD
1
mg/l at flassk level, and 55000 mg/l to 1820 mg/l at 5 l
from an initiall 8000 mg/l to 1500
scale in the laaboratory. The ex
xperiments carriied out at site reesulted in removval of COD
from initial 68800 mg/l to 3967 mg/l at 25 l sccale and from 30000 mg/l to 1400 mg/l at a
pilot plant of ssize 25 m3. The bacteria belongeed to genera Ochhrobactrum, Pseeudomonas,
Sphingomonass, Comamonass, Cupriaviduss, Xanthomonaas, Klebsiella, Bacillus,
Flavobacteriuum, Aeromonas,, Bacteroides, Ralstonia andd Achromobacteer. Except
Bacteroides, R
Ralstonia and Acchromobacter, all
a other genera were found to be
b efficient
in reducing thhe organic load
d and thus indiccated their poteential for bioauggmentation
purpose.
Published on 25tth February 2011
Page 63
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP554
Determination of Reesistance of Listeria
L
monoccytogens Tow
wards
d Antibiotics in Presence oof Tween 80
Saanitizers and
Sh
hahina Shaikh
h, Abhay Raorrane and Dee
epali Giremar
1
Departmentt of microbiologyy New Arts Com
mmerce and Sciennce College, Ahm
mednagar
Listerria spp are ubiq
quitous in nature & express the resistance towardds different
type of sanitizzers and antibiotics in the preseence of tween 80 (polyoxyethellin sorbitan
monolate ). L
Listeria spp. cou
uld secreat extrracellular enzym
me i.e glycosyl transferase
which helps bacteria to increaase the unsaturatiion to saturationn ratio at 370c. Tw
wo isolates
H2V were screeened for deterrmination of reesistance towardds sodium
GG3V & BH
hypochloride , dettole and am
mpicillin. MIC weere determine beefore and after trreatment of
c
by
tween80 and remarkable shift was observedd. Efflux pump activity was checked
mide agar plate assay. Further checking
c
of effluux was confirmeed by PCR
Ethidium brom
for Mdrl geene. Both the isolates showiing susceptibilitty towards Am
mpicillin ,
Gentamycin, R
Roxythomycin, Azithromycin.
A
Key woords
Tween80, MIC, Efflux puump, Mdrl gene,, AST.
Published on 25tth February 2011
Page 64
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP555
Antibaccterial Activitty of Crude Extracts
E
of Sp
pices against Food
Bornee Pathogenic Microorgani
M
sms
Shiikha Malla, Sh
hilpa Mujumd
dar and Madh
huri Amrutkarr
Departmentt of Microbiologyy Modern collegge of Arts, Sciencce & Commerce, Pune 05
Grow
wing concern ab
bout safety of foods
f
led to thhe development of natural
antimicrobialss. Spices are thee most commonnly used antimiccrobials. Spices like clove,
coriander, nuutmeg, cinnamon
n, lemongrass, cardamom, blaack pepper whiite pepper,
cumin, turmerric, ginger exhib
bit antimicrobiall activity. Presennt investigation is focused
on black peppper (Piper nig
grum L.) cuminn (Cuminum cyyminum L), andd turmeric
(Curcuma lonnga.). Crude extrracts were used for determinatioon of antibacterrial activity
and phytochemical screening
g. Solvent extraction was done for cumin (seeeds), black
pepper (seeds)) and turmeric (rhizomes);
(
solveents used were m
methanol, ethanol, acetone
and DMSO. S
Spice extracts in
n methanol & ethhanol showed anntibacterial activvity against
bacterial pathoogens.
Key woords
ma longa.
Antibacteriaal activity, Piper nigrum, Curcum
Published on 25tth February 2011
Page 65
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP556
Evaluaation of Antib
bacterial Actiivity of Pigmeents Produceed by
Pseudomonas
P
aeruginosa
Shilpi.R.Chand & R.B.Vaidya
a
shilpichand@
@yahoo.in
Deppartment Of Miccrobiology, Instittute Of Science, Mumbai 4000322.
o several pathoggenic microbes is the distinctivee colour of
A haallmark feature of
their microbiaal colonies when
n propagated in the clinical labooratory. Such piggmentation
comes in variety of hues and has A hallmarkk feature of seveeral pathogenic microbes
m
is
the distinctivee colour of theeir microbial coolonies when ppropagated in thhe clinical
laboratory. Suuch pigmentatio
on comes in varriety of hues annd has often prooven to be
useful in foodd, cloth, painting
g, cosmetics, phaarmaceuticals annd plastics as ann option for
artificial synthhetic pigments which create seerious environm
ment and safety problems.
Hence the preesent work main
nly focuses on the
t production aand evaluation of
o safe and
natural pigmeents from naturall resources. Thee pigment produucing Pseudomonnas species
were isolated from soil, wateer and contaminnated egg samples on cetrimidee agar and
maintained byy refrigeration att 40c. The isolatees were identifieed by performingg following
tests: macrosccopic and micro
oscopic morphoology motility, Catalase, Oxidaase, HughLeifsons, Caseein hydrolysis, Indole and H2S production.
p
The iisolates were com
mpared for
their pigment production abillity in King’s A,
A Demoss and Franks, Minimaal Salt and
nutrient brothh media by specctrometric method at 520nm. O
Only, two isolattes showed
better pigmennt in King’s A medium
m
compareed to Demoss &F
Franks broth meedium (i.e.:
isolate no-26 and isolate no
o-15). The direcct co -relationshhip between piggment and
biomass produuction of isolatee no-26 in Kingg’s A broth at 3350C on rotary shaker
s
with
200rpm at thee interval of 24 hours
h
was studied and the maxim
mum pigment annd biomass
was at 120th hhour followed by
b decrease after 144 hours. Thhe optimum connditions for
pigment produuction determineed for isolate noo-26 were pH-7.0 and temperatuure-35oC in
presence of gglucose as a C-source, ammoniaa as a N- sourcee and Fecl3 as a source of
iron.The pigm
ment was extractted by Blackwoood and Neish meethod and the anntibacterial
activity of piggment extract waas tested against the common patthogens of food,, water and
cosmetic prodducts by well diiffusion techniquue. The antibacterial activity was
w noticed
against E.colii, K.pneumonae, P.vulgaris, S.fflexnerri, S.aureeus; while no acctivity was
observed agaiinst S.pyogen an
nd P.merabilis. The
T hemolytic aactivity test was performed
using human R
RBC’s to check the suitability off pigment for huuman consumptioon and also
to check the nnon-toxicity of th
he pigment whicch was confirmedd by negative results of the
test. Since piggment showed a good antibacteriial activity and nno blood hemolyysis, it may
be used to disscover new drugs with broad speectrum or it can be used as a foood colorant
in beverages, ccakes or to deco
orate and display the food items.
Published on 25tth February 2011
Page 66
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP557
Biodegraadation of Heexavalent Chromium Usin
ng Microorgaanisms
S
Singh Viveka , Srivastava Shreya
S
and Go
ore Suneeti
Fergusson College, Pune
Hexaavalent Chromiu
um is a toxic, muutagenic and carccinogenic compoound which
is produced as a by-product of
o many industrries like metal plating, dye mannufacturing,
o its high solubiility, it can reacch the undergroound water
leather tannerries etc. Due to
sources and contaminate th
hem. Consumptiion of Hexavaalent Chromium
m at limits
exceeding the EPA standards can lead to seveere damage to thhe kidneys, lunggs, skin and
other organs. It can also leaad to the development of canccers and mutations in the
a
Although Hexavalent C
Chromium is harmful
h
to
genome. It affects plant also.
ms, a large num
mber of them caan tolerate highh concentrationss and even
microorganism
reduce it to tthe non toxic Trivalent
T
Chrom
mium. These include genera like Bacillus,
Pseudomonas,, Arthrobacter etc. The aim of
o our research is to isolate, annalyse and
identify microoorganisms that are
a able to degraade high levels of Hexavalent Chhromium in
water. The saamples taken weere sewage wateer so as to obtaain an indigenouus strain as
most of the inndustrial effluentts are discharge in
i sewage. The sample whose consortia
c
of
microbes had the highest degradation potential for Hexavalennt Chromium was selected
nsortium was plaated to obtain thee individual isolaates. It was
and analysed ffurther. The con
found that onlly one isolate waas responsible foor the degradatioon process. This isolate was
then subjecteed to increasing
g concentrationss of Hexavalennt Chromium inn Davis –
Mingioli Syntthetic medium an
nd the reductionn was checked eeach day up to day
d 10. The
effect of pH and Temperatu
ure on the degraadation potentiaal of the isolatee was also
checked to opptimize the parrameters for rappid remediation of contaminateed sites or
sewage waterss. Results showeed that the isolatte could degradee Hexavalent Chrromium up
to a concentraation of 50 ppm
m with complete and rapid degraadation of 10 pppm and 20
ppm in 2 and 5 days respectiv
vely. The optimuum pH range of the isolate for degradation
d
T optimum temperature for thhe growth of thee organism
was found ouut to be 6 to 8. The
was 37 ºC andd degradation waas faster at 55 ºC
C. This isolate coould be extremelly useful in
remediating ccontaminated sittes due to its abbility to reduce Hexavalent Chhromium at
such high conncentrations and also its removal from sewage water treatment pllants as the
organism is m
mesophilic.
Published on 25tth February 2011
Page 67
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP558
Study of Synthesis
S
of Silver
S
Nanoparticles
Sourabh Mehta*, Rebecca
R
Thom
mbre and Shu
ubhangi Puran
nik.
D
Dept. of Biotechno
ology, Modern College,
C
Shivajinnagar, Pune 05
Silveer nanoparticless were prepared by bacterial reduction, Bioo physical
reduction, andd chemical redu
uction method. The
T formation oof Silver nanopaarticles was
observed usiing UV-Visiblee absorption spectroscopy.
s
T
The formation of Silver
nanoparticles was studed by the typical surfface Plasmon abbsorption maxim
ma at 418pectrum. We haave used energyy dispersive sppectroscopy
420nm form the UV-Vis sp
M) to characterizze the Silver nannoparticles.
(EDX), Scannning Electron microscopy (SEM
The nanopartticles of Silver demonstrated
d
annti bacterial activvity against gram
m negative
bacteria and ggram positive baacteria . The antii cancerous activvity of Silver naanoparticles
was studied onn cancerous cell line The Humann Monocytic celll line (THP1).
Key Woords
Silver nanooparticles, UV-V
Vis spectrum, SE
EM, EDX, Antibacterial, Anticanncerous.
Published on 25tth February 2011
Page 68
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP559
Decolorizaation of Textiile Dye Direcct Lemon Yelllow by Burkh
holderia
Cepaccia
*
Sunil. S. Tiwari and Sa
angeeta. K. Jangam
Mumbai 400 0322
Deppartment of Micrrobiology, Instittute of Science, M
Dischharge of textile wastewater conttaining toxic dyees can adverselyy affect the
aquatic ecosystems and humaan health. This study is part off efforts to deveelop textile
effluent bio ttreatment proceesses to producce reusable watter by decolorizzation and
degradation oof dyes to non toxic metabolitess by bacteria. Inn the present stuudy, a dye
decolorizing bbacterium was isolated
i
from dyye contaminatedd sample and iddentified as
Burkholderia cepacia by 16
6S rRNA sequuencing. The efffect of pH, teemperature,
aeration, % iinoculum, initiaal concentration of dye, initial concentration of various
carbon and nnitrogen sources was studied with an aim tto determine thhe optimal
conditions reqquired for maxiimum decolouriization and deggradation of Dirrect lemon
yellow, whichh was used as a model dye. Thee optimum dye-ddecolorizing actiivity of the
culture was oobserved at pH 7.4 and incubaation temperaturre of 30o C aftter 7 days.
Decolorizationn was confirmed
d by UV-VIS sppectrophotometeer. The initial dyye solution
showed high peak at the wavelength off 410nm. The decolorized dyye showed
a
of peak
p
at 298nm, which indicateed that the
disappearancee of peak and appearance
decolorizationn is due to dye
d
degradationn by Burkholdderia cepacia. Optimum
decolorizationn took place striictly under aerobbic conditions, w
which is contrarry to other
well-documennted reports. Burrkholderia cepaacia showed 99%
% decolorizationn of Direct
lemon yellow in presence of 1% (w/v) glucosse and 98% in ppresence of 1% (w/v)
(
yeast
bility to decolorrize five other sttructurally differrent textile
extract. The isolate had an ab
l
yellow onn Na-alginate beeads and degraddation were
dyes. Adsorpption of Direct lemon
studied. Cellss immobilised in
n 7.5% Na-alginate matrix shoowed lower deccolorization
(30%) as com
mpared to free cellls (77%) and coonsidering the dyye adsorption phhenomenon
on alginate bbeads (11%) th
he efficiency off the two systeems was not foound to be
comparable. P
Phytotoxicity and
d microbial toxiccity studies weree also carried ouut using the
original dye. P
Phytotoxic effecct of Direct lemoon yellow on groowth of Phaseallus mungo,
Triticum aestiivum was studied
d by calculating factors, such as percentage of geermination,
height of shooot and length of leaf.
l
Keywoords:
Direct llemon yellow, Decolorization,
D
B
Burkholderia
ceppacia, Phytotoxiccity.
Published on 25tth February 2011
Page 69
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP660
Phytocheemical and An
ntibacterial Screening
S
of Solvent Extrracts of
Leaves of B
Bauhinia Raccemosa Lam. (Caesalpiniaaceae) againstt Enteric
Bacterial Pathogens
*S.B.Dah
hikar1, 2, S. A. Bhutada2, D.H.Tambekar2, and S. B. Ka
asture1
1
sbdahikar10@
@gmail.com
Departmennt of Pharmaceutical Microbioloogy, Sanjivani Coollege of Pharmaaceutical
Educcation and Reseaarch, Kopargaonn
2
Departm
ment of Microbiology, Sant Gadgge Baba Amravatti University, Am
mravati
Phyytochemical screening of thee plant leaves reveals the prresence of
carbohydratess, alkaloids, flav
vonoids, steroidds, and tannins.. Petroleum ethher extract,
chloroform exxtract, ethyl aceetate extract andd methanol extraacts of leaves of Bauhinia
racemosa Linnn. were prepared and antibacterrial activity weree studied by disc diffusion
method againnst certain en
nteric bacterial pathogens succh as Escheriichia coli,
Staphylococcuus aureus, Klebssiella pneumoniia, Enterobacterr aerogenes, Pseeudomonas
aeruginosa, SSalmonella typh
himurium, Salmoonella typhi, Sttaphylococcus epidermidis
e
and Proteus vvulgaris. The Methanol
M
extractss had wide rangge of antibacterial activity
against enteriic bacterial path
hogens than the petroleum etheer extract, wherre as ethyl
y higher antibaacterial activity than chloroforrm extract.
acetate extracct were slightly
Antibacterial activity of vario
ous extract of leaaves of Bauhiniaa racemosa wass carried in
harmaceutical drrug from naturaal origin for preevention of
attempt to develop a new ph
enteric infectioon.
Key Woords
Antibbacterial activity
y, Bauhinia raceemosa, enteric baacterial pathogenns
Published on 25tth February 2011
Page 70
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP661
Viral D
Diagnosis by Using
U
RT – PCR:
P
A Case Study on Banana
Chlorosis Disease
S. O.
O Gupta1 and B. A. Aglave2*
1
Departmentt of Biotechnolog
gy, Modern Colllege of Arts, Scieence and Commeerce, Pune
2
Departm
ment of Biotechno
ology, H.P.T. Arrts and R.Y.K. Sccience College, Nashik
N
Banaana is an importaant fruit crop of tropical
t
and sub--tropical countriies. Banana
plant has an hherbaceous stem and belongs to the
t family Musaaceae. The wild relatives
r
of
banana contain large, hard seeeds but the dom
mesticated varieties are seedless. Due to the
lack of viable seeds, propagattion is done vegeetatively, by means of corms. Banana crop
is vulnerable tto many diseasess amongst whichh viral diseases aare the major connstraints in
production. Banana Chlorosiss disease was firsst reported in Auustralia (Magee, 1940) and
s
RNA
in India (Joshi and Joshi, 1974). The causativve organism, BSV is a positive sense
plant virus with a tripartite geenome. In the cuurrent investigation RT-PCR techhnique was
o the virus from
m the infected pplant samples. Two
T
sets of
used to detectt the presence of
primer specifiic to coat protein
n gene were proccured from Indiaan Institute of Hoorticultural
Research, Baangalore. Diffeerent parameterrs such as annnealing time, annealing
temperature, fforward and reveerse primer conccentration etc weere used to standdardize the
amplification protocol. The cDNA was am
mplified and thhe product of 700bp
7
was
mpared with thatt of healthy plannt samples
successfully oobtained. The reesults were com
which did nott show any amp
plification with the primers. Thhe current protocol can be
used to screenn a number of corms prior to planntation, thus avooiding heavy lossses.
Key Words
RT - PCR, Banana Chlorosis Disease, Musaceae, R
RNA virus, CMV
V
Published on 25tth February 2011
Page 71
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP662
Biopulp
p - An Eco-Frriendly Apprroach
S. M. Kulka
arni, Navale S.
S K., Sulkshan
ne H. C., Sharrma L. P., Kha
andelwal
S.R., S
Shirke M. D., Kokani
K
N. F., Bhavsar S. P., and Joshi S.
S A.
Department O
Of Microbiologyy, H. P. T Arts annd R.Y.K Science College, Nashik 422005
Evenn in the world off paperless officce, we are forcedd to use papers. Plants are
used as a raw
w material in th
he conventional pulping processs by cooking under
u
high
temperature annd pressure. Thiis process requirres many chemiicals and high ennergy. Due
to extensive destruction of plants, there as been enviroonmental imballance. The
y be explored to help in avoidingg the deforestrattion. In our
microbiology techniques may
project, an atteempt is made to prepare the eco-friendly pulp. B
Biodegradation of
o lignin by
Basidiomycetees, Bacteria and Actinomycetees has been repported. Hence to
t produce
biopulp from lignocellulosic material, we haave screened 14 isolates of Actiinomycetes
m termite guts. Agricultural
A
bypproducts such ass sugarcane
and 7 isolates of Bacteria from
bagasse and soybean industry
y waste were thee two substrates used for the prooduction of
biopulp since they are easily available
a
and cost effective. Thee presence of lignin in high
content producces pitches in the final product i.e. paper. Thus iit is necessary to reduce the
lignin contennt of the raw materials usedd for preparatioon of paper. Solid
S
state
fermentation was performed to get maximuum reduction oof lignin contennt in these
pes of Actinom
mycetes, Bacteriaa and also conssortia were
substrates. Thhe individual typ
inoculated in these substrates to carry out sollid state fermenttation at room teemperature.
nin content was determined by kkappa number. Two
T
of the
After 30 days incubation, lign
Actinomycetees showed decreaase in lignin content upto 0.1% aand 0.356%.The biopulp so
produced was then used to maake eco-friendly paper. The addittion of binding agent
a
while
making paper helps in specificc texture and streength of the finaal product. Whilee preparing
a
binding agents’ viz. Pulluulan, gelatin & Fenugreek
ecofriendly paaper; we have added
powder. The ecofriendly pap
per made using pullulan & gelaatin gave paper with good
A
ccan be exploited to produce
strength and oodorless. Furtherr the isolates of Actinomycetes
Polysaccharidde and Asparginaase.
Published on 25tth February 2011
Page 72
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP663
Plasmid P
Profiling and
d Antibacteriaal Activity off Garlic Extraact and
Clove Oil on Clin
nical Isolates of Pseudomoonas aeruginoosa
Te
ejlaxmi Inamdar, Milky Sa
ahukar and Ka
avita Parekh
Modern C
College of Arts, Science and Com
mmerce, Ganeshhkhind, Pune- 4111053.
Pseuddomonas aerugiinosa is an opporrtunistic pathogeen, and a commoon cause of
nosocomial innfection. It exhiibits an innate resistance
r
to a wide range of antibiotics.
Pseudomonas aeruginosa maaintains antibiottic resistance pllasmids, both ResistanceR
Resistance Transfer Factors. Thee bacterium is nnaturally resistannt to many
factors and R
antibiotics duee to low permeability to outer membrane,
m
constiitutive expressioon of efflux
pumps and prroduction of plaasmid encoded extended-spectru
e
um β-lactamasess (ESBLs).
Resistance is either innate reesistance (chrom
mosomally-encodded) or acquiredd resistance
(plasmid-encooded). Antibacteerial susceptibilitty testing of aquueous extract off garlic and
clove oil agaiinst multidrug reesistant clinical isolates of Pseuudomonas aerugginosa was
done. Garlic is reported to exhibit
e
broad sppectrum antibactterial activity aggainst both
Gram-positivee and Gram-neegative bacteria.. Clove oil is also known too have an
antagonist efffect against Gram
m-negative bacteeria. Different cconcentrations of
o Aqueous
Garlic Extractt and Clove Oil were
w used to cheeck antibacteriall activity on fifteeen isolates
by disc diffussion method. Inh
hibition zones ranging
r
from 8--25 mm by aqueeous garlic
extract and 8--33 mm by clovee oil were obserrved. In additionn plasmid profilinng of eight
isolates was ddone by the mid
di-prep and Kado and Liu methhod. However pllasmid was
not detected inn the isolates tested so far.
Key Woords
Pseudomoonas aeruginosa, Aqueous Garlic Extract, Clovee oil, Plasmid proofiling.
Published on 25tth February 2011
Page 73
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP004
Screenin
ng of Fungal Isolates for Lignolytic
L
En
nzyme, Mangganese
Peroxiides
Udata M. A.*
*, Joshi A. J.1, and Ranjek
kar M. K..2
1
2
D
Department of Microbiology,
Mi
Dayyanand Science College, Latur.
Headd, Department off Microbiology, Dayanand
D
Sciennce College, Latuur.
Ligniin is an insolub
ble, high moleccular weight poolymer. So, varrious fungi
specifically w
white rot fungi deegrades it extraccellularly. The fuungi attack the lignin so as
to fragment itt & diffuse to th
he hyphae & crooss the cell mem
mbrane. Biodegrradation of
lignin is carried out by lignollytic enzyme syystem which includes laccases, manganese
m
peroxidase annd lignin peroxiidase. Manganese peroxidase is heme peroxiddase and it
forms family of isoenzymes. The aim of prresent work is sscreening of thee lignolytic
fungi for prodduction and assaay of manganesee peroxidase usiing the property that it can
oxidize a varieety of phenols an
nd dyes.
Key Woords
Lignolytic enzzymes, Manganeese peroxidase, W
Wood bark.
Published on 25tth February 2011
Page 74
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP664
Screeningg of Lipases as
a Potential Virulence
V
Facctor in Acinettobacter
sspecies Isolateed from Diffeerent Hospitaals in India
Urvi Doshi & Kariishma Pardes
si*
karishma@unnipune.ac.in
D
Department of Miicrobiology, Uniiversity of Pune, Pune 411 007
Lipasses (triacylglyceerol acylhydrolaases; EC 3.1.1.3)) are produced by various
microorganism
ms either alone or
o together with esterases (carbooxylic ester hydrrolases; EC
3.1.1.1). The present study aims at understannding the possibble virulence prroperties of
m chosen for thhe study is Aciinetobacter, knoown as an
these lipases. The organism
gen which has not
n been studiedd much for the variety of
important nossocomial pathog
virulence facttors it possesses. In the present study, we screeened 42 clinical isolates of
Acinetobacterr spp. for prod
duction of lipasses by three ddifferent plate assays
a
viz.
Rhodamine B, Phenol red and
d Tributyrin Agarr Plate assay. Byy Tributyrin Agaar assay, 15
isolates were shown positive for lipase prodduction some of which also couuld be only
mation, Phenol red assay and Rhodamine B assay was
esterase posittive. For confirm
performed. Onn phenol red agaar 8 isolates werre shown to prodduce lipase. By Rhodamine
R
B method thee same 8 isolatees were screeneed positive for llipase productioon. Lipases
produced by tthese isolates weere further quanttified by titrimetrric method. A coomparative
wed that Rhodam
mine B plate asssay at 0.01% of Rhodamine
R
account of theese methods show
B was sensitive and efficientt in screening thhe lipase produccers. Future studdies would
fication, molecular characterizaation & studyinng the potentiall virulence
include purifi
properties of A
Acinetobacter lip
pases.
Key Woords
Acinetobacter, Lipases,
L
Virulennce, Rhodamine B
B, Titrimetry
Published on 25tth February 2011
Page 75
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP665
In Vitro Sttudies of Anttimicrobial Prroperties of E
Extracts from
m Unani
Medicinall Plants
Vais
shali Prabhun
ne, Namra Ghansar and Su
upriya Bhopalle
Abeda Inamdar
I
Senior College,
C
Camp, P
Pune
Naturre has blessed India
I
with immeense variety of m
medicinal plantss which are
being used siince times imm
memorial to curee diseases. Unaani system of medicine
m
is
distinct from oother fields of medicine
m
as the drugs
d
it uses are natural. Being natural
n
and
inexpensive, U
Unani mediciness provide an efffective and safe alternative to thhe existing
medicines. Thhe prime reason
n for these mediicines not being widely known is that the
literature survvey is either in Persian,
P
Urdu or Arabic languagee. Hence there is a need to
provide a scieentific base and documentation for
f this system. Due to emergennce of drug
resistant pathoogens like MRS
SA, skin infectioons are becominng very commonn. The skin
pathogens likee Staphylococcuss aureus, Candidda albicans, Pseeudomonas aerugginosa and
Escherichia coli are responsib
ble for severe skkin infections. T
There is a growiing need to
w antimicrobial compounds witth diverse chem
mical structures and novel
discover new
mechanism of action. Unanii plants produciing wide range of secondary metabolites
m
possessing anntimicrobial pro
operties can be harnessed to ttreat skin pathoogens. For
estimating thee antimicrobial activity of these plants,
p
active priinciples are extraacted using
either crude eextraction or So
oxhlet apparatuss. Different solvvents such as chloroform,
c
ethanol, and m
methanol are em
mployed for this purpose.
p
The exttracts are then suubjected to
well diffusionn assay. Screenin
ng was carried out using methannol extracts from
m plants like
Psoralea coryylifolia, Swertia chirata, Embellia ribes, Rubiaa cordifolia, Naardostachys
jatamansi, Sm
milax glabra, Wriightia tinctoria, Picrorhiza kurrroa, Cyperus rottundus, and
Gymnema syllvestre against Gram
G
positive, Gram
G
negative aand fungal pathoogens. The
methanol extrracts of Psorallea corylifolia and
a
of Embeliaa ribes showed maximum
activity against Candida albicans and Staphyylococcus aureuus while minimuum activity
omonas aeruginosa and Esccherichia coli. Thus, by
was observedd with Pseudo
formulating thhe extracts into medicines Unaani system can pprovide a cure to
t the skin
infections andd prove to be a bo
oon for the socieety.
Key Woords
Unani System
m, Drug resistannce, Antimicrobiaal activity
Published on 25tth February 2011
Page 76
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP666
Lignin Deggrading Perox
xidase in Biobleaching of Paper-Pulp Mill and
Textile Dye-Bassed Effluentss
V. A
A. Tile *: Borkhataria B. V,
V Bhalerao V. B, Aher N. S..
vaishali_tile@yyahoo.co.in
Deparrtment of Microb
biology, K.T.H.M
M. College, Nashik, Pune Universsity
p
is the maj
ajor pollutant froom paper-pulp mill
m effluent
Ligniin, the nature’s plastic
due to its inntense unaestheetic brown coloor, hydrophobiccity and poor mechanical
m
properties, tennds to be a recalcitrant compoound. Textile dyye-based industrries release
colored efflueents due to pressence of large amount
a
of mixtuure of dyes whhich is also
hazardous. Miicrobial extracelllular lignin perooxidase enzyme has a potential to degrade
lignin and a w
wide range of complex
c
aromatic dyestuffs. Froom various envvironmental
niche eight baacterial and two actinomycetes
a
issolates were screeened for lignolyytic activity
out of which tthree bacterial an
nd one actinomyycetes were able to decolorize 444% to 49%
of lignin. Crudde extracts of lig
gnin peroxidase enzyme gave 600% to 76% decoolourization
of methylene blue dye during enzyme assay. The studies on bbiobleaching of paper-pulp
% color reductionn and in case off textile dye- bassed effluent
mill effluent ggave 60% to 75%
50% to 58% decolourization was observed. The heterogeneeous combinationn of lignin
peroxidases fr
from mixed conssortia gave 80%
% to 85% color reduction in treatment of
paper-pulp miill effluent and 70% to 75% deecolourization inn treatment of textile
t
dyebased effluentt which is signiificantly high. This
T
system of lignin peroxidaase may be
efficiently ussed in biobleacching and bioddegradation of effluents from respective
industries.
Key woords:
Lignin peroxxidase, Biobleach
hing, Paper-Pulpp mill effluent, T
Textile dye-basedd effluent
Published on 25tth February 2011
Page 77
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP667
Bacteriaal Synthesis of
o Silver Nanoparticles an
nd Its Applicaations
V. A. Tile*., S. P. Pawar., W. R.
R Sayyed., an
nd A.V. Bhosale
vaishalitile@yyahoo.co.in
Department of microbiiology, K. T. H. M.
M College, Nassik, Pune Univerrsity
The ddevelopment of reliable processees for the syntheesis of silver nannomaterials
is an importannt aspect of nan
notechnology todday. Many synthhetic proceduress for silver
nanoparticles are available, bu
ut a narrow and controlled size ppreparation seem
ms difficult
to obtain becaause it depends on the adjustedd concentration of reacting chem
micals and
controlled reaaction environm
ment. Colloidal metal
m
particles caan be obtained by chemical
synthesis but these methods use
u toxic chemiccals in the synthhesis protocol, which
w
raises
great concern for environmen
ntal reasons. The nanoparticles syynthesized bioloogically are
stabilized direectly in the process by proteins.A
Although it is knnown that microoorganisms
such as bacteeria, yeast, and fungi play an im
mportant role inn the remediatioon of toxic
metals througgh reduction of the
t metal ions, only recently thhis approach is considered
interesting as nanofactories. In
n this study, the production of silver nanoparticlees by some
bacteria is invvestigated by both intracellularlyy and extracelluularly. For intraccellular, the
silver resistantt organisms werre grown in convventional media ccontaining AgNO3, and for
extracellular, tthe test strains were
w
cultivated in
i their special cconventional connditions for
24 hours. AgN
NO3 at concentrration of 10mM
M is separately aadded to the eacch reaction
vessels that coontained the supeernatants of Eschherichia coli, , B
Bacillus subtilis,.. The silver
nanoparticles thus produced were characteriized by transmisssion electron microscopy
m
and UV-visible spectroscopy. The silver nannoparticles weree effectively prooduced, by,
Escherichia ccoli and Bacillu
us subtilis.The prepared
p
silver nanoparticles were
w
in the
range of 12–225 nm with incrreased stability and
a enhanced annti-bacterial pottency. This
effect is dose dependent and is more pronouunced against Grram-negative baacteria than
Gram-positivee organisms. Antifungal efffects of silver nanoparticles on certain
pathogens of the skin were investigated. Nano-Ag
N
showeed potent activiity against
clinical isolattes and ATCC strains of Tricchophyton rubruum , Candida albicans ,
Aspergillus fuumigatous The results
r
showed nano-Ag
n
exertedd activity on thhe mycelial
anatomy and spore germination. Thus, the sttudy indicates thhat the nano-Agg may have
considerable antifungal acctivity, deserving further innvestigation foor clinical
applications.Inn the area of watter purification, nanotechnology
n
offers the possibbility of an
efficient remooval of pollutants and germs. Thhe nanoparticles may be used foor detection
and removal oof chemical and biological
b
substaances.
Key Woords
Silver-Nannotechnology, Baacteria-Nanopartticles, Extracelluular, Intracellularr, TEM,
Antimicrrobial
Published on 25tth February 2011
Page 78
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP009
Study off Extended Sp
pectrum Betaa-Lactamase (ESBL) Prod
ducing
Grram Negativee Bacilli in Faamily Enterobbacteriaceae.
V.S.Vattkar, P. G. Shadija, S. J. Gh
hosh
vsatish999@reddiffmail.com
Departmennt of Microbiolog
gy, Dr. D. Y. Patil Medical Colleege, Kolhapur, 416006.
4
ESBL
L producing Graam Negative Baccilli is a worldwiide problem. Altthough few
studies have rreported on the prevalence
p
of ESBL
E
producers in Indian hospittals, ESBL
producing baccteria may have evolved
e
in severral hospitals all oover the country. Therefore
the study wass carried out at Dept
D
of Microbiiology, D. Y. Paatil Hospital andd Research
Center, Kadam
mwadi, Kolhapu
ur, to examine thee incidence of E
ESBL producing strains and
multiple drug resistance in fam
mily Enterobactteriaceae duringg the period of Dec
D 2007 to
May 2009. A total number off 197 GNB beloonging to familyy Enterobacteriaaceae were
isolated from various clinical samples & studdied for ESBL prroduction by Kiirby- Bauer
LSI (Clinical Labboratory Standaards Institute) sttandards &
disc diffusionn test as per CL
confirmed by using DDST (D
Double Disc Synergy Test). Out of 197 isolates, 83 isolates
SBL producers on the basis oof resistance or decreased
(42.13%) werre potential ES
sensitivity to third generation
n Cephalosporins (3GC). ESBL production is detected
d
by
olates. Seventy (35.53%) isolatees out of these 83 isolates
Jarlier DDST for these 83 iso
he ESBL produccers were tested for MIC andd antibiotic
were DDST ppositive. All th
sensitivity tesst (AST). All th
hese 70 isolatess were susceptibble to Imipenem
m (100%),
Piperacillin-taazobactum (87.5%
%) and Amikaciin (81.2%). Amoong these isolatees E.coli 33
(39.75%) & K
Klebsiella pneum
moniae 16 (37.200%) were most common ESBL producers.
Majority of thhe ESBL produ
ucers were isolaated from the m
medical ward 200 (28.57%)
followed by suurgical ward 16 (22.85%) and from ICU 12 (17.14%).
Key Woords
Extended Sppectrum beta-Lactamases (ESBL
L), 3GC, Resistannce, Enterobacteeriaceae.
Published on 25tth February 2011
Page 79
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP668
Accumu
ulation of Poly
yhydroxyalk
kanoate (PHA
A) from Styreene by
Pseuddomonas spp and Effect off N2 Concentraction on PH
HA
Accumu
ulation
Vid
dya N. Udawa
ant, Smita A. Daule and D.. D. Giramkarr
New Arts Comm. and Sci. College, Ahmeddnagar
A are polyester off various hydroxxyalkanoate whicch are synthesizeed by many
PHA
organisms. Buut Pseudomonass can produce PHA efficientlyy by using toxic aromatic
hydrocarbon S
Styrene, which iss also much resisstant to microbiaal degradation.
Styrene is releeased in million
ns of kilograms in
i a year from ppetrochemical annd polymer
processing inddustries. Styren
ne is a suspected carcinogen,terratogen ,toxic to
t kidneys,
respiratory traact,and nervouss system. PHA accumulated ass discrete granuules in the
cytoplasm of microorganism
m and it acts as storage source of carbon in unbalanced
u
nutrient enviroonment such as nitrogen deficienncy. The converrsion of styrene to PHA by
Pseudomonas spp. Provides a new link betw
ween an aromatiic environmentaal pollutant
and aliphatic PHA accumulattion. PHA posseess properties siimilar to variouus synthetic
thermoplastic polyester. It is used in the production of pplastic utensils and other
disposable iteeams,food packaaging, biodegraddable rubber,in cosmetic,medicine and as
antibiotic. Ourr routinely used petroleum basedd plastics are non biodegradablee and hence
cause environnmental hazards.. But the PHA are
a the bioplastiics which is biodegradable
and can be used as alternativee to conventional plastic for the betterment of ennvironment
and also envvironmentaly haazardous styrenne is convertedd into ecofriendly useful
products. It is important for th
he global commuunity to have an alternative for thhe products
uch as plastic. PH
HAs at least willl be a solution foor the most
derived from ppetroleum oil su
of the industriies and society.
Published on 25tth February 2011
Page 80
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
OP007
Biosurffactant Produ
uction by a Marine
M
Strain
n of Pseudom
monas
1
Vijendra A. Kavatalkar1, Smita S. Bhuyan
B
, Peeh
hu S. Pardesh
hi1 and
1,2
Balu A. Cho
opade *
directoribb@unnipune.ac.in
D
Department of Microbiology,
M
University of Pune,, Pune 411 007
2
Institute oof Bioinformaticss and Biotechnology, University of Pune, Pune 411
4 007
1
A maarine isolate of Pseudomonas
P
aeeruginosa produuced a potent bioosurfactant
o
in PG medium
m at pH 7.2, 30 C at 120 hours. The
T biosurfactannt is a secondary metabolite
and was parttially purified by extraction with
w
chloroform
m: methanol foollowed by
preparative thhin layer chrom
matography. The biosurfactant iis a brown, watter soluble
powder made up of lipid and
d polysaccharidee with a yield oof 1g/lit. The bioosurfactant
lowered the ssurface tension (SFT) of distilled water to 49..377 mN/m, hadd a critical
micelle conceentration of 25
5µg/L and redduced interfaciaal tension (IFT
T) between
kerosene-wateer interface. Parttially purified bioosurfactant exhibbited antimicrobbial activity
against Staphyylococcus aureu
us, Bacillus subttilis, Bacillus puumilus and E. coli.
c
It was
also able to chelate chrom
mate and ferrouus ions indicatting its potentiial use in
bioremediationn. The crude biiosurfactant at concentration
c
5000 µg/ml exhibitted 83.9 %
killing of HeL
La cells. The paartially purified biosurfactant
b
dissrupted 97% Pseeudomonas
aeruginosa 01 and 74% Accinetobacter bauumannii biofilms. Additionally, the crude
biosurfactant aalso had an intense antioxidant activity of 83.555 % per 20 minuutes. Large
number of beneficial traitts make this biosurfactant excellent canddidate for
environmentall and biomedicall applications.
Key Woords
Biosurfacctant, Pseudomonas aeruginosa, SFT, IFT, Antim
microbial, Anticcancer,
Antioxidant, Meetal chelation
Published on 25tth February 2011
Page 81
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP669
Uttilization of Molasses
M
for Biosurfactant
B
t Production
by Streptom
myces S2
Vynk
katesh. M. Sa
anam, Deepalli S. Mane., Ga
auri M. Pawa
ar,
Shilpa. S. Mujumdar an
nd Smita. S. B
Bhuyan*
@gmail.com
smitabhuyan@
Departm
ment of Microbio
ology, Modern coollege of Arts, Sccience & Comm
merce,
Shivajinagar Puune 411 005
Actinnomycetes weree isolated from
m soil and scrreened for prodduction of
Biosurfactant.. Total nineteen
n isolates were obtained, out of which, fivee exhibited
biosurfactant production. Biosurfactant
B
prroduction was carried out inn medium
ole carbon sourcee. Maximum bioosurfactant prodduction was
containing moolasses as the so
shown by Strreptomyces S2. Effect
E
of enviroonmental parameeters such as pH
H, salt and
nitrogen sourcce on biosurfactaant production were
w
checked. pH
H of 7.5 , Pb (00.1g%), Mg
(1g%) &
NaNO3 (1g%) supplementatioon resulted in maximum bioosurfactant
production. Puurification of bio
osurfactant is undder process.
Key woords
B
Biosurfactant, Biioemusification, Actinomycetes, Streptomyces
Published on 25tth February 2011
Page 82
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP770
Isolation
n, Characterizzation and Application
A
off Phenol Degrraders
Uzma T.
T Momin and Varsha K. Va
aidya
Deepartment of Miccrobiology, The institute of Sciennce, Mumbai 32
c
Indusstrial wastewaters containing hiigh concentrationns of phenolic compounds
are dischargedd into the naturaal ecosystem reppresenting seriouus environmentaal problem.
Two isolates w
were obtained by
b selective enriichment techniquue using minim
mal medium
supplemented with phenol (100- 1000 µg/ml). Both the isolaates, identified as
a Candida
guilliermondiii were selected on
o the basis of grrowth response tto increasing conncentration
of phenol andd degradation efficiency.
e
Bothh the strains showed resistancee to heavy
+2
+2
+2
2
+2
metals such ass Ni Cu , Pb and Cd as weell as to various antibiotics indiccating their
potential in bioremediation. Pllasmidial locatioon of genes confe
ferring resistancee to phenol,
w detected in only
o
one isolate by acridine orannge curing.
heavy metals and antibiotics was
Plasmid was isolated by alkaline lysis method and ddetected by aggarose gel
g
transfer by transformatioon was carried out under
electrophoresiis. Horizontal gene
laboratory as well as simulateed conditions. Thhe two isolates w
were used as a consortium
c
to obtain highh degradation effficiency. The coonsortia were evvaluated for theiir ability to
degrade phenool at different pH
H, temperature, organic and inorrganic nitrogen source and
phenol concenntration. Efficien
nt biodegradationn was observed aat pH 7, temperaature 30°C,
nitrogen sourcce (20 mg of N2/mg
/
of phenol) urea,
u
phenol conccentration 1000 µg/ml.
µ
The
consortia in im
mmobilized form
m also degraded phenol.
p
Published on 25tth February 2011
Page 83
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP771
Antibacteerial Activity of Medicinal Plants again
nst Gastrointtestinal
Pathoggens
Pratiibha A Patil , Shilpa S Mujjumdar, Madh
huri Amrutka
ar.
Depaartment of Micro
obiology, Moderrn College, Shivaajinagar, Pune 05
0
Mediicinal plants aree important for the study of ttraditional uses and act as
a new anti-iinfectious agentts. Plants like Neem (Azarddica indica ), tuulsi(Ocium
sanctum), gginger(Zingiber officinale), lem
mon(Citrus aurrantifolia), gaarlic(Allium
sativum),
gguava(Psidium guajava),
leemon grass(Cym
mbopogon citraate) show
antibacterial aactivity .Out of these
t
plants neeem ,tulsi , gingeer ,lemon were taken for
in vitro antibbacterial susceeptibility tests. Solvents likee methanol, ethhanol and
acetone weree used for extrraction .The ethhanolic and accetone extracts exhibited
high antibacteerial activity agaainst Bacillus, S. aureus and Kleebsiella.
Key woords
Solvent extraccts, Medicinal pllants, Antibacterrial activity
Published on 25tth February 2011
Page 84
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP772
Utilizatioon of Petroleu
um Hydrocarrbons by Baccteria Isolated
d from
Soil and Contaminateed With Engiine Oil
Dalvi C. S.,
S Satbhai M.
M A., Bhutada
a S. A.,
Department
D
of Microbiology,
M
New Artss, Commerce and
a Science Coollege,
Ahmednnagar
Envirronmental pollu
ution with petrooleum and petroleum productss has been
recognized ass one of the most serious probleems. Oil is released into the ennvironment
which affects many species of plants and anim
mals as well as humans. Contam
mination of
soil by oil cauuses it to lose itss useful propertiees such as fertiliity, water holdinng capacity,
permeability aand binding cap
pacity. Hence, thhe attempt of stuudy was to inveestigate the
counter measuure to remediatee soil contaminaated with oil. B
Bioremediation provides
p
an
effective and efficient strategy
y to speed up thhe cleanup proceess. For that purrpose three
mechanic worrkshops were sellected where thee soil had been ccontaminated. Sooil samples
were enrichedd using Bushneell-Hass enrichm
ment medium foor a week. A total
t
of 30
bacterial strainns capable of usiing used engine oil as a sole carbbon source were isolated.
These isolates were identified
i
on thee basis of standaard morphologiccal, cultural
and biochemical characteristiics using Bergy’s manual of Deeterminative Baacteriology.
howed prominent oil degrading aactivity. These isolates
i
are
Out of these 330 isolates, 7 sh
Pseudomonas, Serratia, Baciillus, Micrococccus, Proteus andd Alcaligens. Their
T
lipase
n Egg yolk agar, Phenol red agarr and oil powderr agar. The
activity had been observed on
A
MIIDC. (Indian Seeamless Metal Tubes)
T
was
steel industry effluent from Ahrnednagar
analyzed for physico-chemiccal characteristiccs which includde BOD, COD,, DO, pH,
Alkalinity, Chhloride and Phossphate. Along wiith these the furtther study also inncludes the
comparison off oil degrading capability
c
of the isolates obtainedd from soil and the
t bacteria
which are iniitially present in
i the effluent sample and preeparation of carrrier based
biodegrader.
Keywoords
pase activity, Phhvsico-Chemical characterizationn
Oil degradation, Lip
Published on 25tth February 2011
Page 85
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP773
Biodegrad
dation of Com
mmercially Used
U
Insecticides by Soil Isolates
I
B. A. K
Karode, N. B. Chaudhari, S. S. Patil, P. T
T. Patil, and A.
A H.
Jobanp
putra
arpana_jl2@reddiffmai1.com
Department
D
of Microbiology,
M
PSGVPM
M’s, ASC Collegge, Shahada, 4255409
Inseccticide degradatiion is the breakiing down of toxxic pesticides intto nontoxic
compounds annd, in some casses, down to thee original elements from which they were
derived. The most common
n type of degrradation is carrried out in thhe soil by
ms, especially the
t
fungi and bacteria. Insectticides which are
a rapidly
microorganism
degraded are called non-perssistent while thhose which resisst degradation are
a termed
persistent. Usee of persistent in
nsecticides like Malathion, Carbbofuran etc, has resulted in
the entry of thhese insecticides in the ecosysttems. Contaminaation of soil andd water by
such pesticidees is a major env
vironmental conccern. There is a potential carcinoogenic risk
from such inssecticides so there is a serious need
n
to develop remediation proocesses for
their eliminattion or minimiization in the environment. T
The present arrticle is a
constructive aapproach focused
d towards enviroonmental managgement. It highligghts on the
biodegradationn of a pesticide thiamethoxam
t
annd diafenthiruonn. They are broad spectrum
pest controllerrs commonly used nowadays unnder various tradde names for desstruction of
leaf-feeding ppests on cotton, soybean
s
etc. Seriial dilution and ppour plate technniques were
used for screening of organism
ms which could degrade
d
these peesticides. The isoolates were
subjected to a range of conccentrations of peesticides from 110-100 mgL’ inn solid and
liquid media aand the colony forming
f
units annd absorbance inn liquid media reespectively
was investigaated to determin
ne the concentrrations which ccould be toleratted by the
isolates. A com
mparative accou
unt of the isolatess has been depicted in the presenntation. We
further intendd to determine th
he number of daays which wouldd be required foor complete
elimination off the supplied quantity
q
of the pesticides and use the data foor trails on
degradation sttudies in the field
d.
Published on 25tth February 2011
Page 86
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP774
Biodegrada
ation of Dieseel Oil by Soill Isolates
V. R. Ko
othari’ and A.. H. Jobanputtra2*
arpana_j12@reddiffmail.com
‘Department oof Microbiology,, PSGVPM’s, ASSC College, Shahhada, 425409, Nandurbar,
N
Maharashtrra, India
Due tto wide spread use
u of diesel oil,, the natural attennuation, which relies
r
on in
situ biodegradation of pollu
utants, has receeived a large aamount of attenntion. This
inevitably hass hazardous effeects on human life and safety. The parameterrs typically
measured in laboratory testts of bioremeddiation efficacy include enum
meration of
metrically measuuring the quantityy of residual oil. The most
microbial poppulations, gravim
direct measurre of bioremediaation efficacy iss the monitorinng of diesel oil. Microbial
degradation pprocess aids the elimination of spilled oil from
m the environm
ment. Large
amounts of thhe oil can be cleaared by various physical and chemical methods. However,
such methods find limited usee under natural environmental
e
cconditions. Microobiological
ble significance in the past few
w decades. Degrradation of
means have ffound considerab
diesel oil was monitored over a twenty-seven day period usingg gravimetric meethod .Two
P
aeeruginosa and P
Pseudomonas sppecies were
isolates of soiil identified as Pseudomonas
polluted with the oil samples at a loading ratee of 2% (v/w). Thhese soil samplees, together
wo isolates. The rate of oil
with the unpoolluted control saamples were seeeded with the tw
degradation byy these isolates at the interval of
o three days, upp to twenty sevenn days was
thus determineed. There was a consistent decrease in the quanntity of residual oil starting
from the thirdd day continuin
ng till the twennty-seventh day. The approximaate rate of
degradation w
was investigated to
t be approximaately 54 % underr un-optimized coonditions.
Key Woords
Dieseel oil, Gravimetriic, Bioremediation
Published on 25tth February 2011
Page 87
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP775
Raw w
wastes as alteernative med
dia for Trichooderma: towards
sustainable deevelopment
Ru
ushikesh Sank
kpal*, Sunil Deshmukh
D
an
nd Vinay Rale
rushisankpal@
@gmail.com
Deppartment of Miccrobiology, Ferrgusson Collegge, Pune 4110004
Wastte disposal iss of major cooncern in devveloped and developing
d
countries. Esspecially biosolids generated from waste waater treatment plants are
becoming ann environmentaal nuisance. Conventionally thhey are disposeed by land
filling, land spreading, and incineration.. Lots of wastted potatoes arre thrown
directly into waste dumps from
f
vegetable markets and aagricultural landds. Bread,
bread edges, and bread crumbs
c
are diisposed off inn large quantiities from
hospitality aand hotel indusstry. All these wastes need to be disposedd off in a
proper and sccientific manneer. The comforrting fact is thatt these wastes are
a a good
source of miicrobial nutrien
nts. Hence, in this
t study we uused these raw wastes as
growth media for Trichod
derma viride. Trichoderma vviride is a weell known
biofungicide and promotess good plant grrowth in manyy ways. Waste materials
like biosolidds were collected from Puune Municipal Corporation’ss Sewage
Treatment P
Plant, Erandwaane, Pune. Theese biosolids w
were sun-driedd for four
days. Waste potatoes weree collected from
m vegetable m
market while bread edges
urants. Spores of Trichoderm
ma viride were sprinkled
were collecteed from restau
on sterilized raw wastes aseptically, incuubated at room
m temperature for 48-72
owth. The results showed vvery good groowth with
hrs, and obsserved for gro
sporulation ssubstantiating that these wasstes can be veery good mediia for the
Trichodermaa viride. Usin
ng this simplle know-how we can bring about
biobeneficiattion of wastess. Moreover, this
t
techniquee is cheaper thhan other
methods for mass productio
on of Trichodeerma viride. Biobeneficiation of wastes
mising for sustainable development.
is most prom
Key woords
Triichoderma viriide, wastes, bioosolids, bread eedges, potatoes
Published on 25tth February 2011
Page 88
State Level Conference on
Progressivve Education society’s
Miicrobiology in 211st Century
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP776
Charracterization
n and biodivesrsity of Pash
han lake water
Rahul Nimbalkar
N
and
d Varsha Kulk
karni
*Departmennt of Microbiology, Sinhgad College
C
of Sciennce, Ambegaonn Budruk,
Pune-4 1
Pashhan lake is a fresh water man made reeservoir constrructed on
Ramnadi rivuulet of Mutha river. In the past,
p
water from
m this lake wass used for
drinking purppose. At presen
nt this water is not used by M
Municipal Corpooration for
drinking purrpose because of deteriorated water qualitty. This deteriioration is
because of hhuman activities like washing of trucks and dumping of seewage and
industrial w
waste water from
fr
nearby human
h
settlem
ments. At preesent this
ecosystem iss under stress of
o water pollutiion. Present stuudy is to characcterize the
Pashan lake w
water with resp
pect to its physsico-chemical aand biological properties
p
and to study its microbial diversity.
d
Physsico-chemical aand biological properties
p
of any aquatic system and its biodiversityy are influenced by each otheer. Results
way to know whether
w
in futuure this fresh w
water resourcee could be
will pave a w
used again ass a potable watter resource or not?
n
Published on 25tth February 2011
Page 89
State Level Conference on
Progressivve Education society’s
Modeern College of Arts,
A
Science
and C
Commerce, Pune 411005
Miicrobiology in 211st Century
25th – 26th February
F
2011
Absttract Book
Depaartment of Microb
biology
PP777
In
nfluence off Microorgaanisms on IIndian Currrency
Manjiri Mu
ukund Deshpande
Depaartment of Micrrobiology, Prof. Ramakrishna M
More Arts, Comm
merce and
Science coollege, Akurdi 444
The present inveestigation waas carried outt to observe different
types of m
microbial floraa present on Indian curreency (paper notes)
n
in
circulation. Paper notes were collecteed from differrent places off Pune in
sterile plasttic bags, inocculated in peeptone water for 5 min, incubated
i
overnight, tthereafter sub
bculture on diifferent media such as bloood agar,
mannitol saalt agar, and mac-conkey
m
aggar and incubbated for 24 hours.
h
On
the basis of colony characteristics
c
s, Gram’s sstaining, Bioochemical
characteristiics, Enzyme reactions isollates are idenntify, Some coommonly
found microoorganisms are
a Staphylococci, streptoccocci, E-coli, Bacillus
species, kleebsiella species, salmonella species, α- hemolyticc and βhemolytic streptococci, coagulase
c
negaative staphyloococci etc.
Published on 25tth February 2011
Page 90
Author Index
Author
A. H. Jobanputra
A. J Joshi
A. R. Desai
A.V. Pundle
A.V.Bhosale
Aasawari S. Pawar
Page No.
86,87
74
22
39
78
01
Author
Deepali Giremar
Deepali Mane
Dhutekar S.S
Divya Nair
Diwan V.A
G.K.Wagh
Page No.
64
21, 82
55
20
12, 30
63
Abhay Raorane
Abhijit More
Aher N. S
Akshay Borkar
Alka Jagdale
Alok Vyas
Amar D. Jadhao
Amit Sinnarkar
Anil M. Garode
Anita N. Katkade
Anupama Sonawane
Anushka Devale
Aparna Das
Archana J. Marry
Avinash D. Bholay
Avinash Sunder
Ayushi.A.Chaurasiya
B. A. Karode
B.A. Aglave
Balu A. Chopade
Barbuddhe S.B.
Bedare Monika
Bedse,Tanuja D
Bhadale S. V
Bhalerao M.N
Bhalerao V. B
Bhalkikar S.B
Bhavsar S. P
Borkhataria B. V
Borse Priti K
Chavan K.J
D. D. Giramkar
Dalvi, C. S
02, 64
09, 62
77
03
04
20
05
63
06
11
43
04
60
08
11, 13, 17
39
07
86
71
81
02
10
44
15
15
77
12
72
77
13
16
80
85
Gauri Devasthale
Gauri Jadhav
Gauri Pawar
Ghule P.V
Gore Suneeti
Hrishikesh D. Dhamal
Hrishikesh Joshi
Hussain Khokhawala
Indulkar V.S.
Jadhav Sandip R
Jain Reena R
Jayesh A. Patil
Jhilmil Ghoshal
Jogi M.M.
Joshi A. J
Joshi S. A
Jyoti Mantri
Kadam Shradhha D
Kamble. A
Kapadnis B.P
Karishma R. Pardesi
Kaveri S. Palekar
Kavita Parekh
Kawade S.S
Ketki Nalawade
Khadpekar A
Khandelwal S.R
Kokani N. F.
Kulkarni
Kurup Sunila .B
Lalit Lande
Laxmi Nair
M. S. Vrushali
20
37
21, 82
15
67
05
22
41
23
44
24
25
20
25
74
72
08, 50
24
19, 27
19, 27
40, 45, 75
11
73
30
28
29
72
72
15
17
31
39
32
i
De Souza N.A
Deep.V.Barot
Deepak R. Patil
Mahesh R. Ghule
Maid Sneha A
Manasi Karmarkar
Manoj Bhosale
Meenakshi Dhar
Meghana Kukarni
Mehta Khyati
Milky Sahukar
Mokshada Godbole
Monika More
More Bhagyashree V.
19, 27
18
34
34
13
35
03
36
35
46
73
38
37
25
Madhuri A. Udata
Madhuri Amrutkar
Madhuri Londhe
Priyanka Mane
Purnima Ranawat
R.B.Vaidya
Rahatekar Rohini .R
Rahul Brid
Rahul Nimbalkar
Rajendra Choure
Rajesh M. Chomal
Rakhi Tari
Rakte Snehal B
Ranjekar M.K
N. B. Choudhari
N.Rutika
Namra Ghansar
Nasim Kherad
Navale S. K
Neelima Kulkarni
Neha Naik
Neha S. Vora
Nikita L. Lad
Nilesh A. Sonune
Nilesh P. Anandwani
Niruta Y. Killedar
P. G. Shadija
P.S. Patil
P.T. Patil
Pandurang Lahare
Pankaj .S. Picha
Parkar S.D
Patil Jayesh A
Patil N.N
Patil S. S.
Patil S.D
Patil Sham A
Peehu S. Pardeshi
Poojari Pritika
Poorva Dabir
Pradnya Kanekar
86
32
76
36
72
38, 41
39
40
42
63
25
43
79
44
86
41
45
19, 27
25
15
86
16
44
81
46
35
09, 62, 63
Ratnakaran Neena
Ravi V. Kale
Ravindra Baviskar
Rebecca.S.Thombre
Reena A.Dighe
Repe A.B
Riddhi J. Shah
Roma A Chougale
Rushikesh Sankpal
Rutuja.R.Phatate
S. A. Bhutada
S. B. Kasture
S. J. Ghosh
S. M. Kulkarni
S.B.Dahikar
S.O. Gupta
S.P.Pawar
Sabah Shaikh
Sagar Vandra
Salokhe S.G
Samriddhi R. Sharma
Sandeep Gajbhiye
Sangeeta Jangam
Saral Ghosh
Satbhai, M. A
Sayali Mahajani
Seema S.Sarnaik
ii
74
10, 65, 84
33
49
50
07, 66
17
51
89
01, 28, 42, 51
34
52
13
12, 16, 23, 30,
55, 74
46
26
53
29, 56, 68
54
55
40
57
88
56
14, 70, 85
70
79
72
70
71
78
58
59
03
60
61
69
54
85
09, 62
09, 62, 63
Pradnya N. Nadivkar
Prajakta Bhagwat
Prakash R. Thorat
Pratibha A Patil
Priya Bendre
Shilpa Mujumdar
Shahina Shaikh
Sharma L. P
Sheetal .P. Pardeshi
Shikha Malla
64
72
45, 47
65
Sunny Gaikwad
53
Supriya Bhopale
Supriya V. Suryawanshi
Tambekar D. H
Tejlaxmi Inamdar
Udata M.A.
Urvi Doshi
Uzma T. Momin
V. R. Kothari
V.A. Tile
V.S.Vatkar
Vaidehi Dande
Vaishali Prabhune
Varsha Kulkarni
76
25
14, 70
73
74
75
83
87
77, 78
79
39
76
89
Snehal B. Bari
Sonal.P.Bathija
Sonawane H.V
Sourabh Mehta
Srivastava Shreya
Sulkshane H. C
Sumedh S.Deshmukh
47
48
26
84
48
04, 21, 31, 33,
49, 53, 65, 82,
84
52, 58, 59
66
03
72
46
40
04
41
68
23
67
80
04, 21, 31, 33,
49, 53, 81, 82
63
56
03
68
67
72
38, 43
Varsha.Vaidya
Vidhate Pushpa.S
Vidya N. Udawant
Vijendra A. Kavatalkar
Vinay Rale
Vivek N. Bobade
Vrushali Patil
18, 83
17
80
81
36, 88
05
48
Sunakar
Sunil Deshmukh
Sunil Tiwari
61
88
69
Vyankatesh M. Sanam
W.R.Sayyed
Yogesh S. Shouche
21, 82
78
34
Shilpa Sabnis
Shilpi.R.Chand
Shirish Shah
Shirke M. D
Shirode Abhinav
Shradha B. Nadhe
Shradha Bashetti
Shreyas Kumbhare
Shubhangi Puranik
Siddiqui A.G
Singh Viveka
Smita A. Daule
Smita S. Bhuyan
iii
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