Internal safety regulation of DBMV
Département de biologie moléculaire
Végetale
Bâtiment Biophore
CH-1015 Lausanne
Internal safety regulation
DBMV
Prepared by América Fariña (2012)
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GENERAL
In the laboratories, the work of scientific and technical personnel represents certain risks,
which are important to know and understand
The aim of these regulations is to draw the attention of the personnel of DMBV to the dangers
and of risks that can frequently occur in the laboratories.
In general, the scientific and technical personnel are primarily confronted with the dangers
resulting from (mis)handling of cryogenic fluids, biological and chemicals solvents; to these
dangers one must add the usual electric risks, explosion and fire accidents.
FOR ANY EMERGENCY
CALL «115 » 24 hours/days
LIABILITIES
PERSONAL LIABILITIES: EACH MEMBER OF DBMV TAKES CARE TO
ENSURE HER/HIS SECURITY AND THAT OF HER/HIS ENTOURAGE. IT IS
SUPPOSED TO KNOW THE VALID SAFETY REGULATIONS AND TO APPLY
THEM.
THE LEADER OF EACH RESEARCH GROUP IS RESPONSIBLE FOR THE
SAFETY IN HER/HIS OWN LABORATORY.
THE LEADER OF EACH RESEARCH GROUP MUST MAKE SURE THAT
HER/HIS COLLABORATORS RESPECT THE SAFETY REGULATIONS.
Security Officer UNIL
Pascal Baehler
Pascal.Baehler@unil.ch
Phone : 021 692 25 71/2570
Office : 211
Assistant Security Officer UNIL
Patrick Michaux
Patrick.Michaux@unil.ch
Phone : 021 692 25 72/2570
Office : 210
Assistant Security Officer UNIL
Claude Bidlingmeyer
Claude.Bidlingmeyer@unil.ch
Phone : 021 692 2570
Office : 209
Coordinator of safety of DBMV (CSD)
Answerable chemical/biological safety
América Fariña Henriquez Cuendet
America.FarinaHenriquezCuendet@unil.ch
DBMV
Phone : 21 692 42 37/4240
Office : 5442
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Fire Safety Officer (DBMV)
Yvan Burnier
Yvan.Burnier@unil.ch
Phone : 021 692 42 02*/4239
Office : 5449
Responsible for the computer security (DBMV)
Luca Santuari
Luca.Santuari@unil.ch
Phone : 021 692 42 57
Office : 5436
Responsible for the C-LAB (DBMV)
Prof. Yves Poirier
yves.poirier@unil.ch
Phone : 021 692 42 22
Office : 4418
Responsible Toxic Waste DBMV
Yvan Burnier
Yvan.Burnier@unil.ch
Phone : 021 692 42 02*/4239
Office : 5449
First aid
Yvan Burnier
Yvan.Burnier@unil.ch
Phone : 021 692 42 02*/4239
Office : 5449
Health UNIL
Véronique Blanc Audergon
Annick Duperrex
Infirmières
Antropole 1056
accueilsant@unil.ch
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CHEMICAL PRODUCTS: LABELING CHANGE
All chemicals may present a danger.
In Switzerland, the use of chemical substances is principally ruled by:
•
the Law on chemicals products (LChem RS 813.1)
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the Ordinance on chemical products (OChem RS 813.11)
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the Ordinance on the decrease of risk relative to the chemical products (ORRChim RS
814.81)
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the Law on environment protection (LPE RS 814.01)
•
European Inventory of Existing Commercial Substances (EINECS)
•
the threshold limits values at working poste, SUVA *
The Law on chemical products (LChim) stipulates that the user is responsible for taking into
account the safety information given by the supplier and taking the appropriate measures to
protect himself, the work area, and environment against the dangerous properties of the
compounds being used.
The Ordinance on chemical products (OChem) specifies which safety information has to
appear on the labels as warning symbols, according to criteria of dangerous physico-chemical
or toxic effects
In 2009, a new system of classification of hazards and safety labeling known as the Globally
Harmonized System (GHS) was introduced and based on UN recommendations.
GHS aims to harmonize worldwide tha labeling of chemical including transportation
Introduction SGH / UE
By the end of 2010, suppliers will entirely replace the current labeling for pure
substances and in 2015 for mixtures
Definition of a chemical substance
The term “Chemical Substances” refers to chemical elements and their compounds in their
natural state or obtained through any production process.
A substance is a single chemical species, but may contain impurities, stabilizers or isomers.
A mixture of two or more chemical substances is referred to as a “preparation.”
Numbering of Chemical Substances
In European Directive 67/548/CEE and its subsequent adaptations, chemical substances are
classified by INDEX number. Different isomers with different CAS numbers are classified in
the same way for health, safety and the environment, under the same INDEX number.
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CLASSIFICATION AND LABELLING
Hazard pictograms
Exploding Bomb
GHS01
Flame
GHS02
Flame over circle
GHS03
Gas Cylinder
GHS04
Corrosion
GHS05
Skull and Crossbones
GHS06
Exclamation Mark
GHS07
Health Hazard
GHS08
Environment
GHS09
Signal words
A signal word means a word used to indicate the relative level of severity of hazard and alert
the reader to a potential hazard on the label .
The signal words used in the GHS are:
"DANGER" et "WARNING"
Hazard statements H
A hazard statement means a phrase assigned to a hazard class and category that describes the
nature of the hazards of a hazardous product, including, where appropriate, the degree of
hazard..
Precautionary statements P and pictograms
A precautionary statement means a phrase (and/or pictograms)that describe recommended
measuresthat should be taken to minimise or prevent adverse effects resulting from exposure
to a hazardous product, or improper storage or handling of a hazardous product..
Visit the following link for more details about GHS :
http://www.sigmaaldrich.com/sigma-aldrich/safety/safety-center/globally-harmonized.html#el
http://echa.europa.eu/doc/classification/questions_and_answers_clp_20090526.pd
fhttp://ec.europa.eu/enterprise/sectors/chemicals/classification/how-does-clp-work/
Annexe 1. Classification and labaelling GHS
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SAFETY IN THE LABORATY
These general procedures of safety apply to any person, who
works in the laboratories of the DBMV
1. GOOD PRACTICES IN THE LABORATORY
In the laboratory, it is prohibited:
•
to smoke, drink, eat, pipette with the mouth
•
to store food in the refrigerators (cold room)
It is obligatory:
•
to use the protecting material (lab coat, gloves, glasses…)
•
to take off yours gloves whenever you necessitate displacement out of the laboratory
(i.e. before use the telephone handset, before the opening of doors, before the use of
keyboard data, before the use consultation of book or paper, and so on.
•
to work under a hood with the toxic or volatile products in a suitably ventilated
room
•
to store the volatile products far from the sources of heat
•
to carry out handling with genetically modified micro-organisms and the genetic
transformations only under the hoods with laminar flow.
•
to disinfect the hoods with laminar flows before and after uses and to clean them of
any dirty material.
•
to respect the instructions of waste management.
It is desirable:
•
to work in somebody’s presence during night hours and weekends
•
to work in somebody’s presence during night hours and weekends.
•
to work with equipments that are in good state (any anomaly must be reported to the
person in charge) .
•
to wear spectacles (glasses), but not contact lensesto protect the eyes with goggles
while working with UV
•
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to wear low-heeled and closed shoes
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Utilizing biological and/or chemical products in the laboratory
•
Make a stock of the products that are frequently used for current experiments.
•
Stock up only limited quantities of these products.
•
Clearly label the solutions: name and concentration of the product, solvent, name of
preparer and date.
•
Arrange chemicals by families; they should be correctly identified according to their
risks and of their chemical behavior.
Incompatibility of the products
Incompatible products will be stored separately
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They can be stored together
They must not be stored together (in some cases for small quentities exceptions may be
applied9
They must not be stored together
Annexe 2. Storage of Incompatible Chemicals
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Transport of products
Do not ever carry dangerous material by the hands
Transport the material and the bottles (acid, solvents, medium, etc.,) in a suitable container
(storage bin, box, plate…)
Spill of liquids
Every laboratory frequently using chemical substance has at least one emergency set
including :
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A container with a universal absorbent ( convenient for solvents, oils, acids, bases)
•
Two pairs of highly protective gloves
•
Two protective mask with universal filter ( Do not use in case of fire)
•
A brush
Procedure
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Pour a layer of absorbent on the spill (5mm).
•
Let it absorb for a few minutes.
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Using the highly protective gloves ( eventually a protection mask and a brush,
collect the used absorbent and pour it into a container.
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Label the container according to labeling rules.
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Treat the used absorbent as a special waste.
•
Infomr your safety delegate and announce the case on « Event announcemment »
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MATERNITY PROTECTION
Ordinance of the DFE (RS. 822.111.52)
Protection during and after pregnancy
The protection of pregnancy and maternity is an important concern of the UNIL. All the
possible mesures are taken in presumption of danger to the health of the mother and the child
Since 2001 in Switzerland, the expectant mothers has increased protection of health in
relation to pregnancy and maternity. These requirements are derived from the Ordinance of
the DFE on dangerous or painfull activities during pregnancy and maternity.(March 2001,
RS. 822.111.52)
Potential hazards
The following activities are strictly prohibited for expectant or breast-feeding mothers :
•
Exposure to chemical substances with risk phrases H340, H350, H360, H362
•
P263 : Avoid contact during pregnancy/while nursing.
•
Activities with ionizing radiation.
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Activities with magnetic fields higher than 5 Gauss.
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Activities with Group2 micro-organisms and higher.
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Carrying regularly loads heavier than 5 kg and occasionally more than 10 kg.
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Activity at temperatures lower than -5°C or higher than 28°C
This list is not exhaustive. Other hazards are subject to a particular risk analysis of the
workplace and activities of the employee
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We remind you that the first months of pregnancy are the most critical.
Ordonnance du DFE sur les activités dangereuses ou pénibles en cas de grossesse et de
maternité (réf. 822.111.52 )
http://www.admin.ch/ch/f/rs/822_111_52/index.html
Commission universitaire pour la santé et la sécurité au travail romande
http://www.cusstr.ch/repository/35.pdf
2. CHEMICAL RISKS IN THE LABORATORY
2.1.- Propertiess of substances
Chemicals that are likely to present danger can be related to:
Codification of hazard statements (Annexe 3)
Hazard statements are assigned a unique alphanumerical code which consists of one letter and
three numbers.
The lettre H (Hazard statement) is followed by three numbers:
•
The first number identifies the type of hazard.
- "2" for physical hazards
- "3" for health hazards
- "4" for environmental hazards
•
The two following numbers can classify the hazards by intrinsic properties of the
substance or mixture.
- 200 à 210 : explosivity
- 220 à 230 : flammabilit
- ...
Codification of precautionary statements
Precautionary statements are assigned a unique alphanumerical code which consists of one
letter and three numbers.
The letter P (Precautionary statement) is followeds by three numbers::
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The first number identifies the type of precautionary statement.
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- "1" for general precautionary statements
- "2" for prevention precautionary statements
- "3" for response precautionary statements
- "4" for storage precautionary statements
- "5" for disposal precautionary statements
Alwasy remember
that
kills
that
corrodes
that
burns
that
oxidizes
that
explodes
that
is an environnmental
danger
that
is a health hazard
that
empoisonne
pique
2.2.- Prevention : to read the label and instructions to protect yourself
• P201 : Obtain special instructions before use..
• P202 : Do not handle until all safety precautions have been read and understood.
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Know, consult and observe the rules of the labelling to identify the dangers of the
chemicals: symbols of danger, sentences F, R and S (Note1).
Read the cards of safety of the chemicals
Do not mix anything without knowing the consequences!
Choose the least dangerous products as far as possible.
Comply with the rules of the product storage.
Stockez les produits sensibles dans les armoires adéquates
Store the sensitive products in the adequate cupboards.
The handling of each product requires a suitable protection:
1. Air filtering device (protecting hood).
2. Individual protection (glasses, gloves, lab coat).
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Note.1
To obtain more precise information on the toxicity class of considered product for an
experiment, please check with the literature of the product before using it on the following
sites:
http://www.merck.ch
http://www.sigmaaldrich.com
http://www.cusstr.ch/produits
3.- HANDLING OF THE CHEMICAL AND BIOLOGICAL PRODUCTS AND
MANAGEMENT OF THE WASTE
Chemical and biological waste is subjected to a procedure of elimination and transportation
that has been defined by the Schedule on the movements of waste (OMoD, ADR)). 814.610
of June 22, 2005 (applied in cantons on August 30, 2005).
http://www.admin.ch/ch/f/rs/814_610/index.html#fn1 (in French)
Art. 2 List of waste
1. The federal Department of the environment, transport, the energy and of communication
(DETEC) enacts a law understanding a list of waste. It takes into account the list drawn up by
the European Community.
2. It denotes the list of waste as:
a. special waste: due to their composition or their physico-chemical or biological properties
this waste require a set of techniques and particular organizational measures to be eliminated
in a respectful way for the environment, even if the event of movements is inside Switzerland
b. other waste subjected to the control: due to their composition or their physico-chemical or
biological properties this waste require a restricted set of techniques and particular
organizational measures to be eliminated in a respectful way for the environment, even in the
event of movements is inside Switzerland.
ACIDS AND BASES
GHS05
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The contact with concentrated acids and bases can cause serious burns to the skin, mucous
membranes and eyes.
Handling of acids and bases should be carried out with adequate gloves and safety goggles.
The wearing of contact lenses does not represent a sufficient protection against drop
projections of acid or bases. You should work under hood with the concentrated acids.
Never put water in the acid or the base.
In the case of eye/skin contact with acids/bases, rinse it immediately and abundantly
with water.
STORAGE OF ACIDS AND BASES
• The acids and bases must be stored separately.
• Among the acids, isolate nitric acid and perchloric acid from the others.
• One will take additional care to store the oxidizing acids separately, like HNO3, which can
react violently with the flammable organic substances.
The storage cupboards for acids and bases must be ventilated and provided with withholding
storage bins.
ACID AND BASE DISPOSAL
Aqueous waste composed of concentrated mineral acid (pH=1) (nonchromic acid waste)
⇒ Bin : « ACID WASTE pH=1»
Aqueous waste whose pH lies between 2 and 14 (nonchromic alkaline waste)
⇒ Bin : « ALCALIN WASTE pH=2-14»
Destruction of the mineral acids: HCl, HBr, HI, HNO3, HClO4, H2SO4
Progressive neutralization by a diluted mineral base: NaHCO3, Na2CO3, NaOH ou KOH
Verify that the pH is close to 7.
Pour it out in the sink under running water
Destruction of the mineral bases:: NH4OH, NaOH, KOH, Ca(OH)2
Progressive neutralization by a diluted mineral acid HCL ou H2SO4
With Ca(OH)2 use only HCl because the calcium sulphate is insoluble.
Verify that the pH is close to 7.
Pour it out in the sink under running water.
http://www.cusstr.ch/repository/56.pdf
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SOLVENTS
GHS02
With an exception of some halogenous derivatives, all the solvents are flammable.
No solvent must be stored in a refrigerator, or a freezer. The solvents whose flash point
is lower than the temperature of the refrigerator are likely to cause an explosion!!!
GHS01
The solvents can penetrate in the body by 3 ways:
•respiratory tract (due to their volatility),
•cutaneous way (whatever the state of the skin),
•digestive tract (accidental ingestion).
Handling of solvants
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Avoid vapor inhalation: work under hood.
Avoid the contact with the skin: use gloves and lab coat.
Not to transfer a solvent in a bottle having contained another chemicals.
Work far from a source of heat.
Use systems of mechanical aspiration and glass pipettes.
Avoid any discharge towards the sewer.
Limit the losses due to the evaporation (open bottles etc,).
Preserve waste in containers especially envisaged for this purpose.
STORAGE IN THE LABORATORY
The maximum quantity of easily flammable liquids allowed at the place of work is of 15 L
(maximum capacity of the containers 3 L). They must be stored in storage bins in ventilated
cupboards.
The acetic acid is regarded as flammable for the storage.
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WASTE SOLVENTS
Used halogenous and non-halogenous solvents must be stored in separate cans.
Do not pour the acid into the organic solvent waste. Explosion!!!
SGH01
HALOGENOUS SOLVENTS: are regarded as halogenous solvents if they contain more
than 1% mass of halogenous substances. For example:
dichloromethane
1,1-dichloroethane
chloroform (without phenol) ⇒ Bin : « HALOGENOUS SOLVENTS WASTE »
trichlorethylène
tetrachlorethylene
SOLVANT NON-HALOGENES : Exemples
Ethanol
Methanol
Tetrahydrofurane⇒ Bin : « NON HALOGENOUS SOLVENTS WASTE »
Benzene
Acetonitrile
In the case of break out or of accidental discharge of the products, immediately cover them
with absorbing inert material (sand, soil…), which must be in attendance, recover the
products. If discharge occurs, evacuate area immediately, assess risks, eliminate all ignition
sources, isolate pipe work, ventilate area (explosion proof equipment if mechanical means are
used) and contact emergency services. Evacuation and repairs must only be undertaken by
authorized personnel.
http://www.cusstr.ch/repository/58.pdf
PHENOL
SGH06
Soluble in water, very reactive, strong smelling, corrosive, strongly toxic and flammable. It
requires many precautions for handling and storage.
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Store phenol at fresh and ventilated spaces, protected from the sunlight, any source of ignition
or heat and apart from the oxidizing products.
Handling
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•
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To avoid any contact with the skin and eyes.
To avoid vapor inhalation.
Cause burns.
In the case of skin contact, rinse it immediately with a large amount of water during 15
minutes.
In the case of surface contamination, immediately sponge it by absorbing material. Rinse the
contaminated surface with a large amount of water.
If the contamination is significant, evacuation and repairs must only be undertaken by
authorized personnel.
PHENOL WASTE
The chemicals and their residues must be regarded as special waste
Phenol, Phenol/Chloroforme => Bin : « PHENOL/PHENOL-CHCl3 » (Solution)
http://www.caledonlabs.com/upload/msds/5523-f.pdf
http://www.promega.com/msds/France/frenchmsds/Z566(F).pdf
2-MERCAPTOETHANOL (β-MERCAPTOETHANOL)
GHS06
•
•
•
GHS07
GHS09
The substance is irritating to the eyes, skin and the respiratory tracts.
The substance has little effect on the central nervous system.
Do not to throw the residues in the sewer.
Store 2-MERCAPTOETHANOL at fresh and ventilated spaces, protected from the sunlight,
any source of ignition. Keep separated from oxidants, metals.
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Handling
•
•
after contact with the skin: rinse immediately with water.
after contact with the eyes: rinse them abundantly with water and keep eyelids open for
several minutes.
2-MERCAPTOETHANOL WASTE
The chemicals and their residues must be regarded as special waste.
2-Mercaptoethanol
⇒ Bin : « 2-MERCAPTOETHANOL »
In the case of surface contamination, immediately sponge it by absorbing material. Rinse the
contaminated surface with a large amount of water.
If the contamination is significant, evacuate the personnel only with trained operators
equipped with protective gears
http://www.promega.com/msds/France/frenchmsds/Z523(F).pdf
CHLORINE GAS SEED STERILISATION
When performing sterilization by using chlorine gas, firstly seeds put in open Eppendorf
tubes should be placed into a desiccator jar in a fume hood. A beaker containing 100ml of
bleach is then placed in the desiccator next to the seeds. 3ml of concentrated HCL is added to
the beaker to create chlorine gas. The desiccator jar should be quickly sealed and a vacuum
applied. Seeds require to be exposed to the chlorine gas for a period of between three and
sixteen hours. Following release of the gas, the tubes should be quickly sealed and transferred
to a laminar flow hood.
Bleach reacts with hydrochloric acid to release chlorine gas:
NaOCl + 2 HCl → Cl2↑ + H2O + NaCl
Chlorine gas is poisonous to humans - work with proper
ventilation.
Note: Be careful. You are working with chlorine gas. Keep everything in the hood and close
the bell jar top as quickly as possible upon addition of the hydrochloric acid. After
sterilization it is a good idea to leave the beaker of bleach + hydrochloric acid in the fume
hood overnight before dispensing in the sink.
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GHS06 GHS07 GHS09
H: H270-H280-H315-H319-H331-H335-H400
P: P220-P261-P273-P305 + P351 + P338-P311-P410 + P403
Manipulations
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•
•
Eviter tout contact avec la peau et les yeux
Eviter l’inhalation de vapeurs.
Provoque des brûlures.
http://www.cdc.gov/niosh/ipcsneng/neng0126.html
FORMALDEHYDE
GHS06
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•
•
•
GHS07
Avoid vapor inhalation: work under hood.
Avoid contact with the skin: use gloves and lab coat.
Work far from any source of heat.
Use systems of mechanical aspiration and glass pipettes.
Avoid any discharge towards the sewer.
Preserve waste in containers especially envisaged for this purpose.
Handling
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How to eliminate formaldehyde solutions?
a- In the case of accidental spill
Turn off all possible sources of ignition in the laboratory. Wear appropriate personal
protective equipment (glasses, lab coat and butyl or nitrile rubber gloves). If the still is
important, use a suitable respiratory protection. Disperse absorbent over liquid (better a 1:1
mixture by mass of sodium or calcium carbonate and absorbent. Disgard the liquid mixture in
a container and disposed it as hazardous waste.
If the spill is small, place the container in a fume hood. Add cold water (approximately 10 mL
per 1 mL solution of formaldehyde), then slowly add a solution of commercial bleach (25 mL
of bleach to 1 mL solution of formaldehyde). Leave for 20 minutes at room temperature.
Let the heterogeneous mixture to settle and recover the liquid. The residue is considered has a
strong normal waste. After neutralization the liquid may be poured at the sewer
b- Handling of wastes
For large quantities of solutions, disposal must be proceeded by an approved company.
Place the solutions in containers properly labeled.
For small amounts of waste. Wear butyl or nitrile rubber gloves, lab coat and glassess. Under
the hood, slowly add the diluted formaldehyde solution (approximately 10 mL of water to 1
mL of formaldehyde) to an excess of commercial bleach (25 ml of bleach to 1 ml
formaldehyde). Stir for 20 minutes, then discard the solution in the sewerk after neutralization
(pH 5.5 to 8.5).
CRYOGENIQUES
Les gaz qui ne peuvent être liquéfiés à température ambiante par seule augmentation de la
pression sont appelés cryogéniques. A l’état liquides, ou solides, comme dans le cas de la
carboglace, les cryogéniques sont utilisés comme produits de réfrigérations.
LIQUID NITROGEN
Liquid nitrogen has a temperature of -196°C and causes burns by freezing, which can be
extremely serious particularly if the nitrogen reaches the cornea.
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There is also risk of asphyxiation in the case of massive release. This is why it is
recommended to store liquid nitrogen in a sufficiently ventilated room.
The wearing of protective gloves against the cold (blue gloves) and of goggles is obligatory
The prevention rests on:
• Forbidden discarding of nitrogen in any place where its accumulation could be
dangerous (sewers, basements, pits, closed room)
• No white frost on the safety valve,
• Act slowly when you plunge an element in the bath of liquid nitrogen
• Never place nitrogen in closed containers, or in a Thermos bottle: instead use special
broad opening containers.
BURNS FROM LIQUID NITROGEN
*ATTENTION! The temperature of the lesion must be made to body temperature as soon as
possible.
*Rinse the burn with lukewarm water for at least 15 minutes; cover it with a sterile bandage;
consult a doctor.
http://www.cusstr.ch/repository/62.pdf.
OTHER WASTE
Waste containing oxidants ?
MnO4-, ClO4-,
(Cr2O7)2-, Ce4+
⇒ Bin : « OXYDANTS »
(CrO4)2-, (S2O8)2- etc.
Hydrogen peroxide, H2O2 : to dilute and pour in the sink
Waste containing heavy metals ?
Cu2+ , Zn2+, Pb2+ ,
Sn2+, Al3+ , Cd2+
⇒ Bin : « HEAVY METALS»
Fe2+ , Fe3+ etc
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SOLID CHEMICAL WASTE:
It is forbidden to throw potentially dangerous chemicals in household garbage.
•
•
The products that are still labelled will be left in their original package.
The products that are not able to be identified will be left in their package indicating
«UNKNOWN PRODUCT»
3. RADIOACTIVE SUBSTANCES HANDLING
The laboratory of the DBMV is a laboratory of the type C (LAB C) room 5404.1
Activity limits (AL)
Activity limits, specific to each nuclide, are certified for the various laboratories and defined
by the regulation of the protection against radiation. Generally, the authorized limits for the
various laboratories are multiples of the limits authorized in standard laboratory.
Standard Lab : < AL (does not necessitate the authorization)
Lab C : < 100 AL
Lab B : < 10000 AL
Lab A : according to the authorization
Permissible amounts:
In Switzerland, the tolerated limits of annual amount of the exposure of the workers is
20mSv. However, certain exposures are subjected to the different limits:
• The limiting amount is extended to 500 mSv when the exposure relates only to the skin,
feet or hands.
• The equivalent amount of an external irradiation should not exceed 2 mSv on the
exposed surface of the abdomen of the expecting mothers
• The effective amount of incorporated radioactivity should not exceed 1 mSv of the
expecting mothers who are exposed within the framework of their profession.
For more information on protection of the expecting mothers consult:
http://www.chuv.ch/ira/
The women who breastfeed are not allowed working with radioactive substances,
because of harmful radioactive incorporation and contamination.
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Internal safety regulation of DBMV
Obligation of announcement: Any person intending to handle radioactive substances must
announce oneself before to an expert of protection against radiation:
Responsible for radioprotection DBMV:
Prof. Yves Poirier
yves.poirier@unil.ch
Phone : 021 692 42 22
Office : 4418
Safety regulation for Lab C.
The following rules must be strictly respected during the handling of radionuclides
• The access to the laboratory C is forbidden to non-authorized people
• Wearing of the badge, the lab coat and the gloves is obligatory during work with the
radionuclides.
• The direct contact between hands and containers containing radioactive substances must
be minimized
• The ring of control goes under the glove of the hand which generally holds the sample,
sensitive face against the palm
• The dosimeter badge goes on the lab coat to height of the chest sensitive face towards
outside.
• It is forbidden to drink, eat, smoke and use cosmetics in the laboratory.
• In general, it is not advised to station there unnecessarily
• All manipulations presenting a risk of contamination are done on an absorbing paper
surface
• Avoid the formation of aerosol and the dispersion of droplets caused by use of syringe
(filtering etc...)
• Avoid working in the presence of the stock of radioactive substance; store it as soon as the
removal is carried out
• Use of screens and the distance correctly
• Verify frequently radioactive contamination of surface. Localize area of spill. Place
absorbent material over a liquid spill.
• Do not track contamination out of the spill area, if possible. Remove shoes at the edge of
contaminated area.
• It is imperative to control the radioactivity levels before leaving the laboratory.
• In the case of an accident or contamination, contact the Radiation Safety Officer who
will take part in the operations of decontamination. If people were contaminated, he
will warn the :
Institut de radiophysique appliquée (IRA)
Grand-Pré 1
1007 Lausanne
Tél (021) 623 34 34
http://www.chuv.ch/ira/
•
•
At the end of each handling, the objects must be controlled and if necessary
decontaminated or announced
The contamination of any outgoing object of the laboratory must be controlled. The
contaminated objects must stay
DBMV
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Internal safety regulation of DBMV
•
•
•
•
•
•
Verify the contamination of the hands and the working clothes before leaving the
laboratory
Carefully wash the hands at the end of each handling and before leaving the room
To register purchases, use and scrap radioactive substances in the inspection specification
Write the purchases of radioactive substances, utilization and radioactive waste in the
control booklet
The containers containing radioactive substances must carry the symbol of danger
ionizing radiations, the indication of the nuclide, activity, date and the user name
Radioactive liquid must not be thrown in the sinks, except water used for washing steps.
Some commom radioisotopes
Limit of
exemption
Tritium
Carbone-14
Phosphorus-32
Sulfure-35
Calcium-45
6*105 Bq
2*104 Bq
4*103 Bq
4*104 Bq
1*104
Authorized limit in
standard laboratory
3*108 Bq
9*106 Bq
1*106 Bq
7*106 Bq
Lab C limit
3*1010 Bq
9*108 Bq
1*108 Bq
7*108 Bq
Contamination limit of
the surface
1000 Bq/cm2
30 Bq/cm2
3 Bq/cm2
30 Bq/cm2
10 Bq/cm2
At the lowest measurement of a certain activity, called limit of exemption and defined by the
regulation on protection against radiation, a radionuclide is not regarded as radioactive
ORDERING / ELIMINATION
Toute acquisition, utilisation et élimination de substance radioactive doit être enregistrée,
selon les consignes de l’expert en radioprotection.
STORAGE
The radioactive substances are separately stored from other substances. The radioactive
products are separated between them by taking in account their chemical incompatibilities
RADIOACTIVE WASTE.
The radioactive waste or contaminations should not be eliminated before it has been
controlled. The radioactive waste or contaminations should be handled by the authorized
personnel only.
The radioactive waste will be put separately in marked containers « RADIOACTIVE
WASTE » indicating: the isotope, the estimated activity in MBq, the date of the experiment,
the name of the user and number of the laboratory
Solid waste
⇒ « DECHETS RADIOACTIFS SOLIDES»
Liquid waste (polyethylene bin) ⇒ « LIQUID RADIOACTIVE WASTE »
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Internal safety regulation of DBMV
4. GENOTOXIC PRODUCTS
Carcinogens and mutagenes
GHS08
GHS06
Warning, these are the products labeled "Toxic" with the following sentences and more on the
label:
H340 : May cause genetic defects (state route of exposure if it is conclusively proven that no
other routes of exposure cause the hazard)
H350 : May cause cancer (state route of exposure if it is conclusively proven that no other
routes of exposure cause the hazard)
H370 : Causes damage to organs (or state all organs affected, if known) (state route of
exposure if it is conclusively proven that no other routes of exposure cause the hazard)
Expecting mothers must not handle the products marked as follows
H340 : May cause genetic defects (state route of exposure if it is conclusively proven that no
other routes of exposure cause the hazard)
H360 : May damage fertility or the unborn child (state specific effect if known)(state route of
exposure if it is conclusively proven that no other routes of exposure cause the hazard) Peut
nuire à la fertilité ou au foetus
H362 : May cause harm to breast-fed children
Very carcinogenic products: Ethidium Bromide
Acrylamide/Bis-acrylamide !
All the containers containing genotoxic products (pure or in solution) must carry a clear and
undeletable mark «GENOTOXIC DANGER »
Handling of genotoxic products is carried out with double pairs of gloves, safety goggles and
hood.
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Internal safety regulation of DBMV
For the powder handling, a disposable facial cotton mask is recommended
For the very volatile liquids a mask with cartridge is essential
WEIGHING.
If possible, use a balance that is intended to be used for the genotoxic products, exclusively.
To use the system of double-weighing: in a previously weighed bottle, place a quantity of
product and carry out the weighing. Add the quantity of solvent necessary to obtain the
desired concentration. Close the bottle again.
DECONTAMINATION OF THE MATERIAL.
The person must decontaminate dishes before placing them in standard washing route. This
decontamination depends on the used product. The use of disposable dishes is preferable.
STORAGE OF WASTE.
For the pure products or concentrated solutions: preserve the containers of origin and do not
empty them. These containers must be placed in a double sealed unbreakable packing and
immobilized by an absorbent.
One must not throw even diluted solutions of the genotoxic products to the sink: recover them
in containers ⇒ « DANGER GENOTOXIC WASTE (LIQUIDS) »
Solid waste has to be eliminated by packing in a resistant plastic and placing in the container
⇒ « DANGER GENOTOXIC WASTE (LIQUIDS (SOLID) », as well as the already used
pipette tips.
ETHIDIUM BROMIDE: EtBr
GHS06
H : H302-H330-H341
P : P260-P281-P284-P310
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Internal safety regulation of DBMV
Ethidium bromide (C21H20BrN3, CAS-N°: 1239-45-8) is a powerful mutagen that must be
handled with precaution (too much risk concerned). One should wear two pairs of gloves.
Liquid wastw elimination EtBr : Adsorption on activated carbon (Appendix 4)
Treat the solution of EB by activated carbon, effective minimal quantity: 1000 mg of
activated carbon per mg of EtBr.
After checking of the effectiveness of the treatment (UV, colourless solution), decant the
treated solution in the sink under running tap water.
Solid waste containing of the EtBr (contaminated activated carbon) must be stored in suitable
containers: ⇒ « ETHIDIUM BROMIDE».
Depose the gel (packed in a resistant plastic) and gloves in the same container.
Replacing product:
GelRedTM
http://www.biotium.com/product/product_info/Newproduct/GelStains.asp
SYBR SafeTM DNA Gel Stain
The safer ethidium bromide alternative
http://probes.invitrogen.com/products/sybrsafe/
ACRYLAMIDE/BIS-ACRYLAMIDE
GHS06
H : H301-H312-H315-H317-H319-H332-H340-H350-H361f-H372
P : P201-P280-P301+P310-P305+P351+P338-P308+P313
Acrylamide monomer (C3H5NO is neurotoxic poison by the contact. Pipette it with a bulb or
automatic dispenser , work under hood and wear gloves.
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Internal safety regulation of DBMV
WASTE
Preserve waste in the containers envisaged for this purpose ⇒ « DANGER GENOTOXIC
WASTE »
Depose the gel (packed in a resistant plastic) and gloves in the same container.
In the case of surface contamination, immediately sponge it by absorbing material. Rinse the
contaminated surface with a large amount of water.
5. BIOSECURITY
The majority of the micro-organisms used at the DBMV are arranged in group 1.
(Groups of risk of the micro-organisms and the classes of activity, see Appendix 5)
•
The handling of the genetically modified micro-organisms and experiments of genetic
transformation must be only carried out under the laminar flow equipment.
•Before and after use, the hoods must be cleaned with disinfectant.
To avoid any risk of dissemination of genetically modified material (DNA, micro-organisms,
or transformed plant material), the tubes, the pipettes, Petri dishes and tissue paper, which are
contaminated with the genetically modified material, have to be inactivated before
elimination; the same is true for the plant material and the bacterial cultures.
BIOLOGIC WASTE
Liquid biologic waste is treated by 1% sodium hypochlorite (bleach) (or by others
disinfectants whose effectiveness is known) or by autoclaving before being eliminated in the
sink. Liquid biologic waste must not contain chemical or radioactive liquid waste which must
be eliminated like special waste as it has been aforementioned.
Solid biologic waste, : the sharp or cutting objects, which were used for the culture or
handling of biological material are collected in bags or special containers with the initials
“bio-hazard” before being autoclaved.
Decontamination with ethanol , , use an aqueous solution with 70% ethanol to
decontaminate working surfaces, pots and centrifuge rotors.
Do not autoclave the material that has been treated with the sodium hypochlorite.
Vapours of chlorine are dangerous for the users and corrosive for the autoclave.
Each group assigns a person in charge of the autoclave of biological waste.
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Internal safety regulation of DBMV
For informations find more at:
http://www.lyon.inserm.fr/RiskBio/Decontamination/index.html
6. VEGETAL MATERIAL: SOIL AND PLANTS
Sterilization of the vegetal waste: put the soil and plant waste in the yellow bags:
«Bio HAZARD BAG »
Responsable
M. Blaise Tissot
TEL N° 42 4201* - 4239
7. WASTE OF THE PHOTOGRAPHIC DEVELOPERS
Baths containing the developer with solvant ⇒ Bin : « DEVELOPER»
Baths of bleaching/fixations ⇒ Bidon : « BLEACHING/FIXATIONS »
8. ELIMINATION OF THE EMPTY BOTTLES HAVING CONTAINED
CHEMICALS
Rinse the emptied container under running water. Throw the lid and cross out label by a black
marker.
9. SHARP OBJECTS
Razor blades, needles, etc. ⇒ Special Bin : « DECONTAMINATED SHARP OBJECTS »
⇒ DUSTBIN
RESPECT THE USE OF THE DUSTBINS AND THE WASTE MANAGEMENT.
WASTE, PRODUCT, MATERIAL OR PAPERBOARD WILL NOT BE LEFT AT
ANY PLACE, IN THE INTERIOR OR OUTSIDE OF THE BUILDING.
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Internal safety regulation of DBMV
PILES, PAPER, PAPERBOARD, GLASS, SAGEX, LASER CARTRIDGES,
CAPSULE NESPRESO, GAS BOTTLES, ALUMINUM, GLASS AND TINPLATE
HAVE TO BE BROUGHT TO: RECYCLING ROOM BIOPHORE 1930
Additional informations:
www.cheminfo.ch.
www.chemsuisse.ch/
Commission Universitaire pour la Santé et la Sécurité au Travail Romande
http://www.cusstr.ch/index.php
Office fédéral de l'environnement OFEV
http://www.bafu.admin.ch/index.html?lang=fr
SECURITE UNIL
http://www.unil.ch/fbm/page2293_fr.html
Toxicologie Recherche d'informations sur les produits chimiques
http://www.aimt67.org/toxicologie.htm
Fiche de Données de Sécurité VWR
http://fr.vwr.com/app/Header?tmpl=/help/help_recherchefds.htm
Fiches Toxicologiques INRS
http://www.inrs.fr/
Guide Gestion Déchets EPFL
http://www.unige.ch/sciences/chimie/pdf/securite/dechets_epfl.pdf
« La sécurité en laboratoire de chimie et de biochimie » A. Picot, P. Grenouillet, Ed.
Technique et Documentation-Lavoisier, (1992. 424p. ISBN 2-85206-608-4
(A votre disposition Bureau 5442, A. Fariña)
DBMV
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Internal safety regulation of DBMV
EMERGENCY PROCEDURE
The emergengy phone number for any incident ocurring on the UNIL site is 115
The emergency number is active 24 hours/day and 7 day/week and should be called in the
event of any kind of emergency.
1.
2.
3.
4.
5.
Call 115
Give your name and surname
Describe the situation
Give the exact location and the time of the event
Give the number of wounded if appropriate
How to behave in case of fire or explosion
Alarm (fire boutton)
Call 115 or 0118 (Fire brigade)
Save and rescue persons in danger
Close doors and windows
Turn off the gas and remove flammable liquids from
thr danger zone
Stop and secure your lab experiments if possible
Use the extinguisher or extinguishing system
Inform fire brigade (person to rescue, specifics
hazards)
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Internal safety regulation of DBMV
How to behave in case of building evacaution (intermitting horn)
Take with you all your belongings (keys,
coat,…) as you will not be able to reenter the
building immediatly
Follows the exits signs
Use the stairways only
Follow the indications given by the
intervention services, ; they will act as guides
to indicates the meeting place
Do not run, stay calm
Signs
Emergency showers
Emergency Exit
DBMV
First aid location
Eye wash
Meeting place
- 32 -
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Internal safety regulation of DBMV
MEETING POINTS
When fire alarm sounds, calmly evacuate the building. Use the staircases only. Do not panic,
follow the emergency exit signs.
DON'T USE LIFTS.
Everyone should proceed to a designated meeting point where headcounts can be taken.
Evacuation will be supervised by members of the fire brigade.
DBMV
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Internal safety regulation of DBMV
Annexe 1
Allocation of label elements
Physico-chemical hazards
Hazard class
Category
Explosives
Unstables
explosives
Danger
H200: Unstables
explosives
Division 1.1
Danger
H201: Explosive; mass
explosion hazard
Division 1.2
Danger
H202: Explosive; severe
projection hazard
Division 1.3
Danger
H203: Explosive; fire,
blast or projection
hazard
Division 1.4
Warning
H204: Fire or projection
hazard
Warning
H205: May mass
explode in fire
Division 1.5
Division 1.6
Hazard pictogram
Pas de pictogramme
1.5 sur fond orange
Signal Word
Hazard statement
Pas de pictogramme
1.5 sur fond orange
Hazard class
Category
Flammable Gases
Category 1
Danger
H220: Extremely
flammable gas
Category 2
Warning
H221: flammable gas
Hazard class
Category
Flammable aerosol
DBMV
Hazard pictogram
Hazard pictogram
Signal Word
Hazard statement
Signal Word
Hazard statement
Category 1
Danger
H222: Extremely
flammable aerosol
Category 2
Warning
H223: flammable
aerosol
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A. Fariña /2012
Internal safety regulation of DBMV
Hazard class
Category
Oxidizing Gases
Category 1
Hazard class
Category
Gases under
pressure
Hazard pictogram
Signal Word
Danger
Hazard pictogram
Hazard statement
H270: May cause or
intensify fire; oxidizer
Signal Word
Hazard statement
Gaz comprimé
Warning
H280: Contains gas
under pressure; may
explode if heated
Liquefied gas
Warning
H280: Contains gas
under pressure; may
explode if heated
Refrigerated
liquefied gas
Warning
H281: Contains
refrigerated gas; may
cause cryogenic burns or
injury
Dissolved gas
Warning
H280: Contains gas
under pressure; may
explode if heated
Signal Word
Hazard statement
Hazard class
Category
Hazard pictogram
Flammables liquids
Category 1
Danger
H224: Extremely
flammable liquid and
vapour
Category 2
Danger
H225: Highly
flammable liquid and
vapour
Category 3
Warning
H226: flammable liquid
and vapour
Category 4
Warning
H227 : combustible
liquid
Signal Word
Hazard statement
Hazard class
Category
Flammables solids
Category 1
Danger
H228: flammable solid
Category 2
Warning
H228: flammable solid
DBMV
Hazard pictogram
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A. Fariña /2012
Internal safety regulation of DBMV
Hazard class
Category
Self-reactive
substances and
mixtures
Hazard pictogram
Signal Word
Hazard statement
Type A
Danger
H240: Heating may
cause an explosion
Type B
Danger
H241: Heating may
cause a fire or explosion
Type C et D
Danger
H242: Heating may
cause a fire
Type E et F
Warning
H242: Heating may
cause a fire
Signal Word
Hazard statement
Danger
H250: Catches fire
spontaneously if
exposed to air
Signal Word
Hazard statement
Danger
H250: Catches fire
spontaneously if
exposed to air
Signal Word
Hazard statement
Type G
Hazard class
Category
Pyrophoric liquids
Category 1
Hazard class
Category
Pyrophoric solids
Category 1
Hazard class
Category
Self-heating
substances and
mixtures
Category 1
Danger
H251: Self-heating; may
catch fire
Category 2
Warning
H252: Self-heating in
large quantities; may
catch fire
DBMV
Hazard pictogram
Hazard pictogram
Hazard pictogram
- 36 -
A. Fariña /2012
Internal safety regulation of DBMV
Hazard class
Category
Hazard pictogram
Substances and
mixtures, which in
contact with water,
emit flammable gases
Category 1
Danger
H260: In contact with
water releases
flammable gases which
may ignite
spontaneously
Category 2
Warning
H261: In contact with
water releases
flammable gas
Category 3
Danger
H261: In contact with
water releases
flammable gas
Hazard pictogram
Signal Word
Hazard class
Category
Oxidizing liquids
Category 1
Danger
H271: May cause fire or
explosion; strong
oxidizer
Category 2
Danger
H272 May intensify
fire ; oxidizer
Category 3
Warning
H272 May intensify
fire ; oxidizer
Signal Word
Hazard statement
Hazard statement
Hazard class
Category
Oxidizing solids
Category 1
Danger
H271: May cause fire or
explosion; strong
oxidizer
Category 2
Danger
H272 May intensify
fire ; oxidizer
Category 3
Warning
H272 May intensify
fire ; oxidizer
DBMV
Hazard pictogram
Signal Word
Hazard statement
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A. Fariña /2012
Internal safety regulation of DBMV
Hazard class
Category
Organic peroxides
Signal Word
Hazard statement
Type A
Danger
H240: Heating may
cause an explosion
Type B
Danger
H241: Heating may
cause a fire or explosion
Type C et D
Danger
H242: Heating may
cause a fire
Type E et F
Warning
H242: Heating may
cause a fire
Signal Word
Hazard statement
Hazard class
Category
Corrosive to metals
Category 1
Hazard pictogram
Hazard pictogram
Danger
H290: May be corrosive
to metals
Health hazards
Hazard class
Category
Acute toxicity : oral
Category 1
Danger
Category 2
Danger
Category 3
Danger
H301: Toxic if
swallowed
Category 4
Warning
H302: Harmful if
swallowed
Category 5
Warning
H303 : May be harmful
if swallowed
DBMV
Hazard pictogram
- 38 -
Signal Word
Hazard statement
H300: Fatal if
swallowed
H300: Fatal if
swallowed
A. Fariña /2012
Internal safety regulation of DBMV
Hazard class
Category
Hazard pictogram
Acute toxicity : skin
Category 1
Danger
Category 2
Danger
Category 3
Danger
H311: Toxic Fatal in
contact with skin
Category 4
Warning
H312: Harmful in
contact with skin
Category 5
Warning
H313: May be harmful
in contact with skin
Hazard pictogram
Signal Word
H310: Fatal in contact
with skin
H310: Fatal in contact
with skin
Hazard class
Category
Acute toxicity :
inhalation
Category 1
Danger
H330: Fatal if inhaled
Category 2
Danger
H330: Fatal if inhaled
Category 3
Danger
H331: Toxic if inhaled
Category 4
Warning
H332: Harmful if
inhaled
Category 5
Warning
H333: May be harmful
if inhaled
Category
Skin corrosion /
Irritation
Category 1A
Danger
H314: Cause severe skin
burns ans eye damage
Category 1B
Danger
H314: Cause severe skin
burns ans eye damage
- 39 -
Signal Word
Hazard statement
Hazard class
DBMV
Hazard pictogram
Signal Word
Hazard statement
Hazard statement
A. Fariña /2012
Internal safety regulation of DBMV
Category 1C
Danger
H314: Cause severe skin
burns ans eye damage
Category 2
Warning
H315 : Cause skin
irritation
Category 3
Warning
H316: Cause mild skin
irritation
Hazard class
Category
Serious eye damage /
eye irritation
Category 1
Danger
H318: Causes serious
eye damage
Category 2A
Warning
H319: Causes serious
eye irritation
Category 2B
Warning
H320: Causes eye
irritation
Classe de danger
Catégorie
Sensibilisation
respiratoire
Catégorie 1
DBMV
Hazard pictogram
Pictogramme de
danger
Signal Word
Mention
d'avertissement
Danger
- 40 -
Hazard statement
Mention de danger
H334: Peu provoquer
des symptômes
allergiques ou d’asthme
ou des difficultés
respiratoires par
inhalation
A. Fariña /2012
Internal safety regulation of DBMV
Annexe 2
Storage of Incompatible Chemicals
This list includes only the most important examples. These chemicals react violently when
they come into contact with each other and must not be stored together.Mixing incompatible
chemicals in a waste container can form an explosive mixture, for example, nitric acid and
ethanol. If a bottle broke in a waste storage area where incompatibles were present, the results
could be disastrous. Remember: incompatible bottles of wastes should be stored separately.
The objective is to avoid accidents in the laboratory.
Subtance
Incompatible with
Acetic acid
Chromium oxide, nitric acid, perchloric acid, peroxides,
permanganates, alcohol, ethylene glycol
Hydroxyl-containing compounds e.g. ethylene glycol, perchloric
acid
Concentrated nitric acid and sulphuric acid mixtures, hydrogen
peroxide
Chlorine, bromine, fluorine, copper, silver, mercury
Calcium hypochlorite, oxidizing agents
Water, carbon tetrachloride and other halogenated alkanes, carbon
dioxide, halogens. (Do not use water or foam extinguishers for
fires involving these metals)
All oxidizing agents, acids, alkalis, halogenated hydrocarbons,
peroxides
water
Mercury (e.g. in pressure gauges), chlorine, calcium hypochlorite,
iodine, bromine, hydrogen fluoride
Acids, powdered metals, flammable liquids, chlorates, nitrates,
sulfur, fine-particulate organic or combustible materials
Nitric acid, hydrogen peroxide
Any reducing agent
Acids
see chlorine
Water
Calcium hypochlorite, other oxidants
Ammonia, acetylene, butadiene, butane, methane, propane,
hydrogen, petroleum benzine, benzene, powdered metals
Ammonium salts, acids, powdered metals, sulfur, fine-particulate
organic or combustible substances
Acetic acid, naphthalene, camphor, glycerol, petroleum benzine,
alcohols, flammable liquids
Acetylene, hydrogen peroxide
Acids, both organic and inorganic
Acids
Ammonium nitrate, chromium (VI) oxide, hydrogen peroxide,
nitric acid, sodium peroxide, halogens, chromic acid.
Extremely aggressive; store separately! Isolate from everything!
Hydrogen peroxide, nitric acid, any other oxidant
Acetic anhydride
Acetone
Acetylene
Activated carbon
Alkali metals
Aluminium
Aluminium alkyls
Ammonia, liquid or gas
Ammonium nitrate
Aniline
Arsenic materials
Azides
Bromine
Calcium oxide
Carbon activated
Chlorine
Chlorates
Chromium (V1) oxide,
chromic acid
Copper
Cumene hydroperoxide
Cyanides
Flammable liquids
Fluorine
Hydrazine
DBMV
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A. Fariña /2012
Internal safety regulation of DBMV
Subtance
Incompatible with
Hydrocarbons butane,
propane, benzene etc.
Hydrogen fluoride
Hydrogen peroxide
Fluorine, chlorine, bromine, chromium (VI) oxide, sodium
peroxide
Ammonia (laboratory gas or solutions)
Copper, chromium, iron, metals and metal salts, alcohols, acetone,
organic substances, aniline, nitromethane, combustible substances
(solid or liquid)
Fuming nitric acid, oxidizing gases
Acids, activated carbon
Acetylene, ammonia (laboratory gas or solutions)
Acetylene, ammonia
Acids
Acetic acid, aniline, chromium (VI) oxide, prussic acid, hydrogen
sulfide, flammable liquids and gases
Inorganic bases, amines
Silver, mercury, mercury salts
Acetic anhydride, bismuth and its alloys, alcohols, paper, wood,
grease. oil, (all organics)
Acids (organic and inorganic), avoid friction, store cold.
Sulfur, compounds containing oxygen, e.g. chlorates, air, oxygen
Alcohols, strong bases, water
Carbon tetrachloride,carbon dioxide, water
see chlorate
see chlorate
Glycerol, ethylene glycol, benzaldehyde, sulfuric acid
Hydrogen sulphide
Hypochlorites
Iodine
Mercury
Nitrates and nitrites
Nitric acid
Nitroparaffins
Oxalic acid
Perchloric acid
Peroxides, organic
Phosphorus
Phosphorus pentoxide
Potassium
Potassium chlorate
Potassium perchlorate
Potassium
permanganate
Silver
Sodium
Sodium peroxide
Sulphides
Sulphuric acid
Sulphur
Zinc
DBMV
Acetylene, oxalic acid tartaric acid, ammonium compounds
see alkali metals
Methanol, ethanol, glacial acetic acid, acetic anhydride,
benzaldehyde, carbon disulfide, glycerol, ethylene glycol, ethyl
acetate, methyl acetate, furfural
Acids
Potassium chlorate, potassium perchlorate, potassium
permanganate
Metals, all oxidizing agents
All oxidizing agents, acids, alkalis, halogenated hydrocarbons,
peroxides
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A. Fariña /2012
Internal safety regulation of DBMV
Annexe 3
List of hazard statements H
Physical hazards
H200
H201
H202
H203
H204
H205
H220
H221
H222
H223
H224
H225
H226
H227
H228
H240
H241
H242
H250
H251
H252
H260
H261
H270
H271
H272
H280
H281
H290
Unstable explosive
Explosive; mass explosion hazard
Explosive; severe projection hazard
Explosive; fire, blast or projection hazard
Fire or projection hazard
May mass explode in fire
Extremely flammable gas
Flammable gas
Extremely flammable material
Flammable material
Extremely flammable liquid and vapour
Highly flammable liquid and vapour
Flammable liquid and vapour
Combustible liquid
Flammable solid
Heating may cause an explosion
Heating may cause a fire or explosion
Heating may cause a fire
Catches fire spontaneously if exposed to air
Self-heating; may catch fire
Self-heating in large quantities; may catch fire
In contact with water releases flammable gases which may ignite spontaneously
In contact with water releases flammable gas
May cause or intensify fire; oxidizer
May cause fire or explosion; strong oxidizer
May intensify fire; oxidizer
Contains gas under pressure; may explode if heated
Contains refrigerated gas; may cause cryogenic burns or injury
May be corrosive to metals
Health hazards
H300
H301
H302
H303
H304
H305
H310
H311
H312
H313
H314
H315
H316
H317
H318
H319
H320
H330
H331
H332
H333
DBMV
Fatal if swallowed
Toxic if swallowed
Harmful if swallowed
May be harmful if swallowed
May be fatal if swallowed and enters airways
May be harmful if swallowed and enters airways
Fatal in contact with skin
Toxic in contact with skin
Harmful in contact with skin
May be harmful in contact with skin
Causes severe skin burns and eye damage
Causes skin irritation
Causes mild skin irritation
May cause an allergic skin reaction
Causes serious eye damage
Causes serious eye irritation
Causes eye irritation
Fatal if inhaled
Toxic if inhaled
Harmful if inhaled
May be harmful if inhaled
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A. Fariña /2012
Internal safety regulation of DBMV
H334
H335
H336
H340
H341
H350
H351
H360
H361
H362
H370
H371
H372
H373
May cause allergy or asthma symptoms of breathing difficulties if inhaled
May cause respiratory irritation
May cause drowsiness or dizziness
May cause genetic defects (state route of exposure if it is conclusively proven that no other routes of
exposure cause the hazard)
Suspected of causing genetic defects (state route of exposure if it is conclusively proven that no other
routes of exposure cause the hazard)
May cause cancer (state route of exposure if it is conclusively proven that no other routes of exposure
cause the hazard)
Suspected of causing cancer (state route of exposure if it is conclusively proven that no other routes of
exposure cause the hazard)
May damage fertility or the unborn child (state specific effect if known)(state route of exposure if it is
conclusively proven that no other routes of exposure cause the hazard)
Suspected of damaging fertility or the unborn child (state specific effect if known)(state route of
exposure if it is conclusively proven that no other routes of exposure cause the hazard)
May cause harm to breast-fed children
Causes damage to organs (or state all organs affected, if known) (state route of exposure if it is
conclusively proven that no other routes of exposure cause the hazard)
May cause damage to organs (or state all organs affected, if known)(state route of exposure if it is
conclusively proven that no other routes of exposure cause the hazard)
Causes damage to organs ( state all organs affected, if known) through prolonged or repeated exposure
(state route of exposure if it is conclusively proven that no other routes of exposure cause the hazard)
May cause damage to organs ( state all organs affected, if known) through prolonged or repeated
exposure (state route of exposure if it is conclusively proven that no other routes of exposure cause the
hazard)
Environmental hazards
H400
H401
H402
H410
H411
H412
H413
Very toxic to aquatic life
Toxic to aquatic life
Harmful to aquatic life
Very toxic to aquatic life with long lasting effects
Toxic to aquatic life with long lasting effects
Harmful to aquatic life with long lasting effects
May cause long lasting harmful effects to aquatic life
List of precautionary statements
General precautionary statements
P101
P102
P103
If medical advice is needed, have product container or label at hand
Keep out of reach of children
Read label before use
Prevention precautionary statements
P201
P202
P210
P211
P220
P221
P222
P223
P230
P231
P232
P233
P234
DBMV
Obtain special instructions before use
Do not handle until all safety precautions have been read and understood
Keep away from heat/sparks/open flames/hot surfaces – No smoking
Do not spray on an open flame or other igntion source
Keep/Store away from clothing/…/combustible materials
Take any precaustion to avoid mixinn with combustibles
Do not allow contact with air
Keep away from any possible contact with water, because of violent reaction and possible flash fire
Keep wetted with …
Handle under inert gas
Protect from moisture
Keep container tightly closed
Keep only in original container
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A. Fariña /2012
Internal safety regulation of DBMV
P235
P240
P241
P242
P243
P244
P250
P251
P260
P261
P262
P263
P264
P264
P270
P271
P272
P273
P280
P281
P282
P283
P284
P285
P231+232
P235+410
Keep cool
Ground/bond container and receiving equipment
Use explosion-proof electrical/ventilating/light/…/equipment
Use only non-sparking tools
Take precautionary measures against static discharge
Keep reduction valves free from grease and oil
Do not subject to grinding/shock/…/friction
Pressurized container – Do not pierce or burn, even after use
Do not breathe dust/fume/gas/mist/vapours/spray
Avoid breathing dust/fume/gas/mist/vapours/spray
Do not get in eyes, on skin, or on clothing
Avoid contact during pregnancy/while nursing
Wash skin thoroughly after handling
Wash hands thoroughly after handling
Do not eat, drink or smoke when using this product
Use only outdoors or in a well-ventilated area
Contaminated work clothing should not be allowed out of the workplace
Avoid release to the environment
Wear protective gloves/protective clothing/eye protection/face protection
Use personal protective equipment as required
Wear cold insulating gloves/face shield/eye protection
Wear fire/flame resistant/retardant clothing
Wear respiratory protection
In case of inadequate ventilation wear respiratory protection
Handle under inert gas. Protect from moisture
Keep cool. Protect from sunlight
Response precautionary statements
P301
P302
P303
P304
P305
P306
P307
P308
P309
P310
P311
P312
P313
P314
P315
P320
P321
P322
P330
P331
P332
P333
P334
P335
P336
P337
P338
P340
P341
P342
DBMV
IF SWALLOWED:
IF ON SKIN:
IF ON SKIN (or hair):
IF INHALED:
IF IN EYES:
IF ON CLOTHING:
IF exposed:
IF exposed or concerned:
IF exposed or you feel unwell:
Immediately call a POISON CENTER or doctor/physician
Call a POISON CENTER or doctor/physician
Call a POISON CENTER or doctor/physician if you feel unwell
Get medical advice/attention
Get Medical advice/attention if you feel unwell
Get immediate medical advice/attention
Specific treatment is urgent (see … on this label)
Specific treatment (see … on this label)
Specific measures (see … on this label)
Rinse mouth
Do NOT induce vomiting
If skin irritation occurs:
If skin irritation or a rash occurs:
Immerse in cool water/wrap in wet bandages
Brush off loose particles from skin
Thaw frosted parts with lukewarm water. Do not rub affected areas
If eye irritation persists:
Remove contact lenses if present and easy to do. continue rinsing
Remove victim to fresh air and keep at rest in a position comfortable for breathing
If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable
for breathing
If experiencing respiratory symptoms:
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A. Fariña /2012
Internal safety regulation of DBMV
P350
P351
P352
P353
P360
P361
P362
P363
P370
P371
P372
P373
P374
P375
P376
P377
P378
P380
P381
P301+310
P301+312
P301+330+331
P302+334
P302+350
P302+352
P303+361+353
P304+312
P304+340
P304+341
P305+351+338
P306+360
P307+311
P308+313
P309+311
P332+313
P333+313
P335+334
P337+313
P342+31
P370+376
P370+378
P370+380
P370+380+375
P371+380+375
Gently wash with soap and water
Rinse continuously with water for several minutes
Wash with soap and water
Rinse skin with water/shower
Rinse immediately contaminated clothing and skin with plenty of water before removing
clothes
Remove/Take off immediately all contaminated clothing
Take off contaminated clothing and wash before reuse
Wash contaminated clothing before reuse
In case of fire:
In case of major fire and large quantities:
Explosion risk in case of fire
DO NOT fight fire when fire reaches explosives
Fight fire with normal precautions from a reasonable distance
Fight fire remotely due to the risk of explosion
Stop leak if safe to do so
Leaking gas fire – do not extinguish unless leak can be stopped safely
Use … for extinction
Evacuate area
Eliminate all ignition sources if safe to do so
IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician
IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell
IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
IF ON SKIN: Immerse in cool water/wrap in wet bandages
IF ON SKIN: Gently wash with soap and water
IF ON SKIN: Wash with soap and water
IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin
with water/shower
IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell
IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for
breathing
IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position
comfortable for breathing
IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if
present and easy to do – continue rinsing
IF ON CLOTHING: Rinse immediately contaminated clothing and skin with plenty of water
before removing clothes
IF exposed: Call a POISON CENTER or doctor/physician
IF exposed or concerned: Get medical advice/attention
IF exposed or you feel unwell: Call a POISON CENTER or doctor/physician
If skin irritation occurs: Get medical advice/attention
If skin irritation or a rash occurs: Get medical advice/attention
Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages
Get medical advice/attention
Call a POISON CENTER or doctor/physician
In case of fire: Stop leak if safe to do so
In case of fire: Use … for extinction
In case of fire: Evacuate area
In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion
In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of
explosion
Storage precautionary statements
P401
P402
P403
P404
P405
P406
DBMV
Store …
Store in a dry place
Store in a well ventilated place
Store in a closed container
Store locked up
Store in a corrosive resistant/… container with a resistant inner liner
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A. Fariña /2012
Internal safety regulation of DBMV
P407
P410
P411
P412
P420
P422
P402+404
P403+233
P403+235
P410+403
P410+412
P411+235
Maintain air gap between stacks/pallets
Protect from sunlight
Store at temperatures not exceeding … °C/… °F
Do not expose to temperatures exceeding 50 °C/122 °F
Store away from other materials
Store contents under …
Store in a dry place. Store in a closed container
Store in a well ventilated place. Keep container tightly closed
Store in a well ventilated place. Keep cool
Protect from sunlight. Store in a well ventilated place
Protect from sunlight. Do not expose to temperatures exceeding 50 °C/122 °F
Store at temperatures not exceeding … °C/… °F. Keep cool
Disposal precautionary statements
P501
DBMV
Dispose of contents/container to …
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A. Fariña /2012
Internal safety regulation of DBMV
Annexe 4
Extractor® EtBr system
Ethidium Bromide (EtBr) Waste Reduction System
The Extractor system is a one-step filtration funnel device for the rapid removal of ethidium
bromide from gel-staining solutions.
This disposable unit contains an activated carbon matrix, which removes >99% of ethidium
bromide from electrophoretic buffer quickly and easily. Each device can decontaminate up to
10 litres of gel staining solution. After filtration, the decontaminated solution can be safely
poured down the laboratory drain.
The Extractor funnel device fits most standard laboratory flasks and bottles (neck size 3345mm), and the unit includes a cap for storage between uses. The polypropylene housing is
chemically resistant to organics. Also included in the package are glass fibre prefilters, which
remove gel peices and other debris to avoid premature clogging of the carbon filter.
Ordering Information - Extractor® Ethidium Bromide (EtBr ) Waste Reduction System
DescriptionQuantity/PackCatalogue Number
Extractor - Starter Pack210 448 030
Extractor - Standard Pack610 448 031
http://www.whatman.com
DBMV
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A. Fariña /2012
Internal safety regulation of DBMV
Annexe 5
DBMV
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A. Fariña /2012