Making Spectroscopy Brighter CUVETTE HOLDERS COLLIMATING LENS FILTER HOLDERS OPTICAL FILTERS FLOW CELLS REFLECTANCE STANDARDS SAMPLING ACCESSORIES www.sarspec.com ACCESSORIES Cuvette Holder - CH • • • Built for 10 mm pathlength cuvettes Suitable for Absorbance and Fluorescence 6 mm collimating lens with adjustable SMA connector • • • Cover included for photosensitive samples Rack for four cuvettes and optional direct attach Small, flexible and affordable Sarspec’s Cuvette Holder - CH is a flexible accessory that can be used to perform Absorbance/Transmittance and Fluorescence measurements. Each configuration is easily interchangeable for both types of measurement and can optionally be performed simultaneously. The combination of Sarspec´s spectrometers and light sources with Cuvette Holder allow users to select the application just by moving the SMA connector into a different position. Absorbance configuration Sarspec’s Cuvette Holder includes a rack specially built for productivity improvement – with the CH-RACK the common spilling of a sample is less probable. This special rack holds four 10 mm pathlength cuvettes. Cover to reduce stray light and avoid the degradation of photosensitive samples is also included. Fluorescence configuration The cuvette holder can be ordered with a direct attach option that increase significantly the sensitivity in Fluorescence measurements. This option connects the cuvette holder directly to the light source maximizing the light intensity on the sample. Parameter Value Applications Absorbance/Transmittance, Fluorescence or both Wavelength range 180–2000+ nm Z Dimension 15 mm Fiber connection SMA 905 (adjustable position) Pathlength 10 mm Collimating lenses 6 mm UV Fused Silica f=10 mm Number of ports 3 (0º, 90º, 180º) Dimensions 85 (L) x 80 (D) x 45 (H) mm Dimensions with rack attachment 85 (L) x 100 (D) x 45 (H) mm Ordering Information Cuvette Holder CH Direct Attach for CH CH-DA WWW.SARSPEC.COM 1 ACCESSORIES Multipurpose Cuvette Holder - CH-MP • • • • • • Built for 10 mm pathlength cuvettes Absorbance, Fluorescence and Raman measurements 12.7 mm collimating lens with adjustable SMA connector Flexibility – 4 entry/exit ports Optional signal enhancing mirrors Optional direct attach SARSPEC CH-MP is a flexible cuvette holder as it’s small, portable and a complete solution for Absorbance, Transmittance, Fluorescence and Raman measurements. The 12.7 mm collimating lenses with adjustable SMA connector allow the illumination of larger sample area and collection of more light, making it the best solution for low light measurements. This cuvette holder is very flexible since it has four ports with M18 threads allowing the user to choose where to connect the collimating lens and optional signal enhancing mirrors. On every port there are slots for slide filters that can be easily coupled for light rejection. The filters, large diameter lens and optional signal enhancing mirrors (CH-MP-EM) combined make this product a great solution for the best results in Fluorescence and Raman measurements. The Cuvette Holder can be ordered with a direct attach option to increase significantly the sensitivity in Fluorescence and Raman measurements. This option connects the cuvette holder directly to the light source maximizing light intensity on the sample. Parameter Value Applications Absorbance, Transmittance, Fluorescence, Raman Wavelength range 180-2000+ nm Fiber connection SMA 905 (adjustable position) Pathlength / Z Dimension 10 mm / 15 mm Collimating lenses 12.7 UV Fused Silica f = 25 mm Number of ports 4 (0º, 90º, 180º, 270º) Filter Slot 4 (max. filter size Ø 12.7 x 3 mm) Dimensions 100 (L) x 100 (D) x 62 (H) mm (with cover) Ordering Information Cuvette Holder Multipurpose CH-MP Direct Attach for CH-MP CH-MP-DA Signal enhancing mirrors (set of 2) CH-MP-EM WWW.SARSPEC.COM 2 ACCESSORIES Peltier Cuvette Holder - CH-QPOD® 2e (Quantum Northwest, Inc.) • Magnetic stirring and dry gas purge • Several Packages available: Absorbance, Fluorescence • Peltier temperature control • Temperature range from -30 to +110 ºC • USB or wireless Bluetooth control or Multipurpose The qpod 2e is a complete sample compartment for fiber optic spectroscopy, including a Peltier-controlled cuvette holder with magnetic stirring, and fused silica lens systems with SMA fiber optic connectors. The qpod 2e housing contains a PID temperature controller that is operated via Bluetooth wireless or USB using the computer program Q-Blue. The qpod 2e is available in three different packages. The CUV-qpod 2e-ABSKIT provides collimating optics to pass light straight through for absorbance, transmittance or turbidity measurements. The CUV-qpod 2e-FLKIT provides imaging lens systems for excitation and detection of fluorescence emission at right angles. The CUV-qpod 2e-MPKIT is a multipurpose kit with a combination of optics that may be used for either absorbance or fluorescence measurements. The qpod 2e is constructed of a single molded housing. The cuvette holder in the center provides temperature control and variable speed magnetic stirring, and holds optical slits to limit light into the samples. A dry gas purge may be used to avoid condensation when working at low temperatures. SMA fiber optic connections and high quality fused silica optics may be inserted in different combinations into optical ports located in the walls surrounding the cuvette holder. Accessories such as polarizers and filter holders may be inserted in the light paths. Spherical mirrors may be used to enhance excitation or emission signals. All optics provide focusing and position adjustments for maximizing signal throughput. Parameter Value Temperature Range -30º C to +110º C Temperature Precision ±0.01º C Temperature Accuracy ±0.15º C Probes accepted Standard 400 and 500 series thermistor Standard cuvette size 12.5 mm x 12.5 mm O.D. Cuvette Z height 8.5 mm Ordering Information QPOD 2e cuvette holder base unit CH-QPOD2e QPOD 2e absorbance/transmission measurements CH-QPOD2e-ABSKIT QPOD 2e for fluorescence measurements CH-QPOD2e-FLKIT QPOD 2e for either absorbance or fluorescence CH-QPOD2e-MPKIT AR-coated fused-silica collimating lens CH-QCL-UV AR-coated fused-silica imaging lens doublet CH-QIL-UV Spherical mirror plug with steering plate CH-QMP Filter holder for 12.5 mm diameter filter CH-QFH Polarizer for mounting on the QIL-UV lens system CH-QPOL-47-215 WWW.SARSPEC.COM 3 ACCESSORIES Collimating Lens • Used to focus or collimate a light beam • Two options: 6 mm and 12.7 mm diameters • Standard UNS 1/4’’-36 for SMA connection • Adjustable SMA connector The main characteristic is the ability to create a well-directed beam with a welldefined diameter, allowing therefore a uniform illumination of the sample in study by converting a divergent beam into a parallel beam. For this to occur, the light emitting element must be placed at the focus of the lens. On the other side, if the incident light is already collimated the lens will work like a focusing lens. All of our collimators covers the UV-VIS-NIR range and have a two-part housing of aluminium, a screw to adjust the position of the SMA connector, allowing users to adjust the focus. Parameter Standard CL Large diameter CL Lens diameter 6 mm 12.7 mm Lens confocal length 10 mm 25 mm Lens material Threads UV Fused Silica UNF 3/8’’-24 and UNS 1/4’’-36 for SMA Wavelength Range M18 and UNS 1/4’’-36 for SMA 180-2000+ nm (UV/Vis/NIR) Ordering Information CL1 CL2 In-line Filter Holder • Connect two optical fibers via SMA 905 • Use up to three 3 mm thick filters • Set for 12.5 mm / 1/2” diameter filters • Includes two CL1 collimating lenses Sarspec’s In-Line Filter Holder (ILFH) can be used with up to 3 filters with 3 mm thick x 12.5 mm / 1/2” diameter and includes 2 collimating lenses CL1 and 2 spacers. With the filters available from Sarspec it is possible to choose the right illumination or specific wavelengths to irradiate or collect light. The two SMA 905 connectors allows the coupling with two optical fibers. The filters are easily replaceable by unscrewing the two-part housing. Please check our filter section for the available filters for this accessory Parameter Value Lens diameter 6 mm Filters diameter 12.5 mm / 1/2” (max. 3 with 3 mm thick) Lens material UV Fused Silica Connectors SMA 905 Wavelength Range 180 - 2000+ nm (UV/Vis/NIR) Ordering Information ILFH WWW.SARSPEC.COM 4 ACCESSORIES Optical filters • Large number of options available • Round unmounted filters for In-line Filter Holder • Slide mounted filters to use with Sarspec’s accessories • 12.5 mm x 3 mm round filters as standard The optical filters available can be purchased as round unmounted filters or as slide filters for an easy coupling. Slide Filters are filters mounted in aluminium bars used to insert the slots available in Sarspec’s Light Sources and Cuvette holders. This makes it very easy to change between filters without disassembling the light sources. Sarspec has available a large number of optical filters including : Longpass Filters – Have low transmission in the shortwave region (blocking band) and high transmission in the longwave region (pass band). The range between 225 nm and 1000 nm has very steep transmission edges. Shortpass Filters – The pass band of shortpass filters is at lower wavelengths than the blocking band. Basically they block longer wavelengths. Bandpass Filters – Have a transmission band or bands surrounded by blocking bands allowing only a portion of the spectrum to pass. They are useful for high transmission in their designed spectrum while blocking other wavelengths. Neutral Density Filters - Reduce the intensity almost equally in a specific wavelength range. Parameter Value Material SCHOTT® filter glass Diameter 12.5 mm (25 mm for LS-Xe300) Thickness 3 mm (variable in Neutral Density Filters) Available filters LongPass Filters (λ @ T=50%) 280 / 305 / 395 / 435 / 495 / 550 / 590 / 645 / 695 / 780 / 850 nm ShortPass Filters (Type) BG3 / BG38 / BG39 / KG1 / KG3 / KG5 BandPass Filters (Type) BG7 / BG18 / UG1 / UG5 / UG11 Neutral Density Filters - Absorptive (T% Vis) 50% / 25% / 5% / 0.5% / 0.1% Ordering Information Unmounted Slide LongPass Filters LPF-XXXX SLPF-XXXX-YYY ShortPass Filters SPF-XXXX SSPF-XXXX-YYY BandPass Filters BPF-XXXX SBPF-XXXX-YYY Neutral Density Filters NDF-ZZZ SNDF-ZZZ-YYY XXXX: Wavelength (nm) or Type ZZZ: % Transmission (50; 25; 5; 0.5; or 0.1) YYY: CHM for CH-MP / LSX for LS-Xe300 / LSW for LS-W / LSP for LS-DWHP / LSM for LS-MIR WWW.SARSPEC.COM 5 ACCESSORIES Flow cells • Available for Absorbance or Fluorescence in stainless • Sapphire windows for wide spectrum transmission steel or PTFE • Easy coupling with SMA905 connectors • Compatible with standard HPLC fittings • Standard pathlength of 10 mm SARSPEC Flow Cells are the ideal choice for the analysis in a continuous flow system. Fluorescence They are available in stainless steel or PTFE according the applications, with 10 mm pathlength and sealed sapphire windows in each of the fiber optic connectors. Our flows cells allow two types of measurement techniques: Absorbance and Fluorescence. For Absorbance, cells are designed in Z shape with 10 mm pathlength and for Fluorescence the cells are designed with a 90º configuration between excitation and emission. Both configurations are designed to provide optimum flow, maximum light intensity and minimum bubble entrapment. Absorbance The robustness of this system is also granted by the use of Sapphire windows for wide wavelength range transmission (180-5000 nm) and great resistance to mechanical ware and harsh chemicals. Each flow cell is delivered with the required sapphire windows, SMA connectors, chemically resistant seals, 1/16’’ (Absorbance) or 1/8’’ (Fluorescence) O.D. tube fittings and 10 cm tubing. Parameter Value Applications Absorbance, Fluorescence Cell Material Stainless steel, PTFE Window material Sapphire Wavelength range 180-5000 nm Internal volume (optical path) 7.85 µl for Absorbance, 31.4 µl for Fluorescence Tubing diameter I.D. - O.D. (Absorbance / Fluorescence) 0.04” [1. 02] - 1/16’’ [1.59] / 0.08” [2.03] - 1/8’’ [3.18] Tube fittings Nuts and Ferrules for 1/16’’ or 1/8” O.D. tubing Optical connectors Standard SMA 905 Ordering Information Flow Cell, 10 mm pathlength, Absorbance, Stainless Steel FCASS Flow Cell, 10 mm pathlength, Fluorescence, Stainless Steel FCFSS Flow Cell, 10 mm pathlength for Absorbance, PTFE FCAPE Flow Cell, 10 mm pathlength for Fluorescence, PTFE FCFPE Sapphire window, 5 mm for flow cell SW5 WWW.SARSPEC.COM 6 ACCESSORIES Reflectance Standards • For diffuse and specular reflectance • Wavelength range from 250 to 2500 nm • 50 mm diameter • Anodized aluminium housing Sarspec has available two types of reflectance standards, for diffuse and specular reflectance. The specular reflectance type consists of a 50 mm mirror with fused silica substrate coated with Al-MgF2 for high specular reflectivity in a wavelength range from 250 to 2500 nm. For diffuse reflectance all Sarspec’s standards use optical PTFE which consists of a pure polytetrafluorethylene (PTFE) disc with 50 mm diameter. As a result of its incomparable whiteness and purity, optical PTFE achieves virtually ideal Lambertian reflectance properties with a reflectivity coefficient of up to 98% in a broad wavelength range from 250 to 2500 nm. Additional advantages when using optical PTFE is its high UV stability, universal chemical resistance and being easily cleaned. Sarspec offers one specular standard and three diffuse standards corresponding to reflectivities of 98%, 50% and 10%. There’s also the possibility for customization for different sizes or reflectivities. Parameter Diffuse Reflectance Specular Reflectance Material Optical PTFE Fused silica / Al-MgF2 Diameter 50 mm 50 mm Thickness 10 mm 12 mm Wavelength Range 250-2500 nm Max. Temperature 260º C Housing 80º C Black anodized aluminium Ordering Information Diffuse Reflectance Standard, PTFE, 98% Reflectance DRSTD98 Diffuse Reflectance Standard, PTFE, 50% Reflectance DRSTD50 Diffuse Reflectance Standard, PTFE, 10% Reflectance DRSTD10 Specular Reflectance Standard SRSTD WWW.SARSPEC.COM 7 SARSPEC, LDA RUA 28 DE JANEIRO 350 B2 4400-335 VILA NOVA DE GAIA TEL. +351 223 709 107 INFO@SARSPEC.COM WWW.SARSPEC.COM