07/06/2011 IPN-Dept ITP/IPB Trend of Alternative Food P Processing i T Technologies h l i (based on IFT’s Report) Purwiyatno Hariyadi ITP530 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 1 07/06/2011 IPN-Dept ITP/IPB Purwiyatno Hariyadi/ITP530 Alternative Food Processing Technologies : Microwave and Radio Frequency Ohmic and Inductive Heating High Pressure Processing P l d El Pulsed Electric t i Fi Field ld High Voltage Arc Discharge Pulsed Light Oscillating Magnetic Fields Ultraviolet Light Ultrasound X-Rays • • • • • • • • • • Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 2 07/06/2011 IPN-Dept ITP/IPB Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 3 07/06/2011 IPN-Dept ITP/IPB Purwiyatno Hariyadi/ITP530 PENGGUNAAN GEL MIKRO (MICROWAVE/MW) UNTUK KEPERLUAN INDUSTRI : Gelombang mikro Berpeluang Penggunaan MW Berdekatan dan perlu diatur mengganggu Tumpang tindih oleh badan proses Dengan kisaran yang berwenang, komunikasi Gelombang radio Mis. Di AS : Federal Communication Commission memperbolehkan pemakaian 4 frekuensi MW : 22150, 5800, 2450 dan 915 MHz Paling banyak : 915 dan 2450 MHz Inggris : 915 dan 2450 MHz Jerman : 27,12; 433,92 dan 2450 MHz Eropa Timur: 2375 MHz Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 4 07/06/2011 IPN-Dept ITP/IPB PEMANASAN DIELEKTRIK (& GEL MIKRO) dipantulkan oleh metal diserap (diubah menjadi panas) oleh dielektrik : internal heating (Molecular friction). δ OδH δ+ δ+ H +/Generator (Oscilator) Bahan pangan Hδ+ H δ+ “Perub. Orientasi polarisasi” Oδ- +/- + + Ionic displacement Purwiyatno Hariyadi/ITP530 Listrik Konversi Energi Panas P = 2π(ε ( o)( ε’)) tan δE2f P = 5.56x10-13 (ε’) tan δE2f P = jumlah panas yang diproduksi per satuan volume [=] W/m3 εo= permitivity of free space ε’ = konstanta dielektrik (sifat fisik bahan yang berhubungan dengan polaritas atau Σ dipole) δ = loss angle E = kekuatan medan listrik [=] volts/m f = frekuensi (hz, s-1) ε’ tan δ = ε’’ = loss factor Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 5 07/06/2011 IPN-Dept ITP/IPB LOSS FACTOR ∈ε” = f (SUHU, FREKUENSI) Mashed Potatoes 35 140 Cooked carrots 25 120 20 100 15 80 60 10 H2O 5 0 450 MHz 160 30 -20 0 20 SUHU 40 60 40 900 MHz 20 2700 MHz MH 0 -20 (oC) 20 40 SUHU 60 (oC) Purwiyatno Hariyadi/ITP530 DAYA PENETRASI GEL MIKRO = d d= λo 2π ε' tanδ λo = panjang gelombang Kedalaman penetrasi, d[=] cm Kadar air ε’ 915 MHz 2450 MHz tinggi sedang rendah 15 4 1,5 8,4 11,7 22,1 3,1 4,4 8,2 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 6 07/06/2011 IPN-Dept ITP/IPB PENINGKATAN SUHU DALAM PRODUK Jumlah panas yang diperlukan untuk meningkatkan suhu produk sebesar ΔT Q = mc ΔT Q = ρVcΔT atau ΔT= Q/ρVc dimana V = volume Jumlah panas yang diproduksi oleh pemanasan gel mikro : Q = PVΔt Q = (5.56 x 10-13 ε” E2 f)VΔt Jadi, ε” E2 f Δt ΔT = 5.56 x 10-13 ρc c untuk pemanasan gel mikro yang sama, ΔT = ≈ ε” = f (Komponen bahan pangan) ρc homogenitas pemanasan = homogenitas bahan pangan Purwiyatno Hariyadi/ITP530 Konfigurasi sistem sterilisasi/pasteurisasi dgn pemanasan dielektrik dan gelombang mikro MW Generator Waveguide Treatment Chamber Conveyor belt MW generator (magnetron) : merubah energi listrik menjadi gel. Mikro Waveguide : memfocuskan pancaran m.w (terowongan dari tabung Al) Treatment chamber: ruang tertutup/terlindung oleh logam Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 7 07/06/2011 IPN-Dept ITP/IPB KARAKTERISTIK PEMANASAN GEL MIKRO • • • • Memanaskan daerah yang mengandung air Pemanasan berlangsung cepat Tidak menyebabkan “gosong”pada permukaan Mudah/Praktis APLIKASI SPESIFIK PEMANASAN GEL. MIKRO : • Cocok untuk mengeringkan bahan setengah kering Contoh : Pasta - waktu pengeringan turun dari 8 jam menjadi 90 menit - jumlah bakteri 15 kali lebih kecil - tidak mengalami case hardening Biji-bijian : terutama untuk Benih - meningkatkan laju germinasi Mengeringkan bagian dalam (tanpa overcooked dipermukaan) Purwiyatno Hariyadi/ITP530 APLIKASI PEMANASAN GEL. MIKRO (UMUM): •Rumah tangga : microwave oven •Komersial 4Pemanasan tanpa merubah sifat-sifat dasar produk : th i & defrosing thawing d f i (tempering) (t i ) 4Pemanasan dengan merubah sifat-sifat dasar produk : Pengembangan adonan & pemanggangan Pemblansiran buah/sayuran : inativasi enzim Pemasakan (cooking) Roasting (untuk kacang-kacangan) 4Pengeringan : Dehidrasi pada tekanan normal Dehidrasi pada tekanan vakum 4Inaktivasi Mikroba : Sterilisasi & Pasteurisasi (kurang sukses ! : masih dalam penelitian!) Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 8 07/06/2011 IPN-Dept ITP/IPB APLIKASI PEMANASAN GEL. MIKRO PADA PROSES PEMANGGANGAN Utama : ~ membantu proses pengeringan lanjut Proses pemanggangan dimulai dengan oven tradisional (mengg. udara panas) : Efektif untuk produk dengan kadar air tinggi Dengan semakin menurunnya kadar air : efektifitas oven menurun case hardening?! Pengeringan/pemanggangan selanjutnya : oven gel. Mikro mengeringkan bag dalam (tanpa “overcooked” di permukaan) Purwiyatno Hariyadi/ITP530 APLIKASI PEMANASAN GEL. MIKRO PADA PROSES THAWING : ☺ Konduktivitas panas air < konduktivitas panas es Proses pencairan : menurunkan proses pindah panas ☺ Loss factor air > loss factor es Proses pencairan : menaikan kadar air dan loss factor >>mempercepat p p proses p pemanasan p ☺ Problem : Pada bahan baku yang ukuran besar - proses pencairan tidak seragam - mengakibatkan overcooked pada bagian ttt Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 9 07/06/2011 IPN-Dept ITP/IPB APLIKASI PEMANASAN GEL. MIKRO PADA PROSES DEFROSTING : Menaikan suhu produk beku: -20oC menjadi -3oC Untuk daging dan mentega mempermudah penanganan (slicing) Minimum overcooked Cepat : ....................> daging dapat didefrost selama 10 menit (tradisional: beberapa hari pada cold room) Minimum perubahan phase Minimum drip loss (kehilangan karena penetesan) Mutu meningkat: lebih higienik, lebih cepat, dapat dilakukan di dalam box (pengemas) Ruang yang diperlukan sedikit Ekonomis Purwiyatno Hariyadi/ITP530 APLIKASI PEMANASAN GEL. MIKRO PADA PROSES DEHIDRASI : VS. PEMANASAN TRADISIONAL/UDARA PANAS : Pindah panas turun : thermal conductivity turun pada bahan pangan kering Semakin lama waktu pengeringan mutu sensori dan mutu gizi turun Oksidasi tinggi : mengakibatkan warna dan vitamin menurun. Untuk produk dengan kadar pufa tinggi, terjadi ketengikan Case hardening : perubahan karakteristik permukaan >> keras, susah ditembus oleh panas/uap air (kualitas produk menurun) Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 10 07/06/2011 IPN-Dept ITP/IPB APLIKASI PEMANASAN GEL. MIKRO PADA PROSES DEHIDRASI : VS. PEMANASAN GELOMBANG MIKRO : Memanaskan bahan dari dalam : Tidak ada masalah ttg konduktivitas panas Tidak memanaskan udara : mrengurangi ksidasi rendah Tidak terjadi case hardening (pindah massa/uap air .........> lancar) Umumnya dipakai untuk mengeringkan semi/partly dried foods, foods dimana gel. Mikro akan tetap memanaskan daerah yang masih basah, tanpa mempengaruhi daerah/bagian yang sudah kering. Mahal Purwiyatno Hariyadi/ITP530 APLIKASI PEMANASAN GEL. MIKRO : LAIN-LAIN Masih dalam taraf penelitian: Blansir, Pasteurisasi, Sterilisasi PENGARUH GELOMBANG MIKRO TERHADAP BAHAN PANGAN : tidak ada pengaruh langsung pada mikroorganisme waktu proses menurun retensi gizi lebih baik (prinsip HTST) Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 11 07/06/2011 IPN-Dept ITP/IPB PEMANASAN OHMIC elektroda BAHAN S P = I2 R P= I2ke-1 I = keEL-1 Power Supply P : laju jumlah panas yang diproduksi per satuan volume (W.m-3) E : kekuatan medan listrik (Volt cm-1) ke : konduktivitas listrik (ohm-1/m, S/m) I : densitas arus listrik (amps/m2) R : tahanan listrik (ohm-1) - kecepatan pemanasan tergantung pada nilai ke bahan pangan - ke bahan pangan =f(kadar air, garam ionik dan asam) - ke bahan pangan cair >> ke bahan padat - minyak dan lemak mempunyai nilai ke sangat rendah Purwiyatno Hariyadi/ITP530 PERBANDINGAN ANTARA PEMANASAN GEL MIKRO DAN OHMIC Kriteria Konduktivitas listrik (siemen/m) Generasi Panas untuk medan listrik 20 V/m (W/cm3) Kenaikan suhu (oC/sec) Pemanasan Gel Mikro 0.25 0.25--4 1-16 0.25 0.25--4* Pemanasan Ohmic 0.005--1.2 0.005 0.02 0.02--5 0.004 0.004--1.2* * kenaikan suhu di permukaan kaleng pada proses pemanasan retort adalah sekitar 0.2oC/sec Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 12 07/06/2011 IPN-Dept ITP/IPB Arah perambatan panas, Q Q(r) = Pr 2 T − T0 = r Pr 2 P 4k 2 ⎡ ⎛ r ⎞ ⎤ − ⎢1 ⎜ ⎟ ⎥ ⎝ R ⎠ ⎥⎦ ⎢⎣ k = konduktivitas panas Kenaikan suhu maksimum Tmax − To = PR 2 4k Kenaikan suhu ratarata-rata T − T0 = PR 2 8k Purwiyatno Hariyadi/ITP530 Contoh : Sebuah bahan berbentuk silinder dengan diameter 2R dan panjang L. Berapa Δ voltase (E) yang perlu diberikan supaya terjadi peningkatan suhu di pusat bahan sebesar (Tmax-T0)oC, dimana suhu awal = T0. J Jawab b: Gunakan persamaan peningkatan suhu PR 2 4k I2R 2 kE − T0 = , I= e 4k ek L Tmax − T0 = Tmax 2 ⎛ k eE ⎞ 2 ⎟ R ⎜ ⎛ E2R 2 L ⎠ ⎝ = ⎜⎜ Tmax − T0 = 2 4k ek ⎝ 4L Jadi, ⎛L ⎞ k E = 2⎜ ⎟ ⎝ R ⎠ k e T0 ⎞⎛ k e ⎞ ⎟⎟⎜ ⎟ ⎠⎝ k ⎠ T0 (Tmax − T0 ) Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 13 07/06/2011 IPN-Dept ITP/IPB Vs PEMANASAN OHMIC & GELOMBANG MIKRO Mirip dengan pemanasan gel. Mikro : Konversi enegi listrik menjadi energi panas Penetrasi panas/daya penetrasi : tidak terbatas Suhu dalam bahan pangan merata (∇ (∇T ≈ 0) Tidak perlu “pengadukan” Cocok untuk memanaskan bahan pangan cair dgn partikulat : sop dll. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 14 07/06/2011 IPN-Dept ITP/IPB Nilai konduktivitas listrik beberapa bahan Bahan nilai ke (s/m) Air murni (25oC) Asam sulfat (25oC) 5,7x10-6 1 kentang(19oC) wortel (19oC) kacang kapri (19oC) daging sapi (19oC) Larutan pati (5 (5,5%, 5% 19oC) + garam 0.2% + garam 0,55% + garam 2% 0.037 0,041 0.17 0,42 0,34 1,3 4,3 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 15 07/06/2011 IPN-Dept ITP/IPB KONFIGURASI SISTEM PEMANAS OHMIC Elektroda 4 Elektroda 3 Elektroda 1 Elektroda 2 Elektroda 2 Elektroda 1 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 16 07/06/2011 IPN-Dept ITP/IPB Purwiyatno Hariyadi/ITP530 (ULTRA) HIGH PRESSURE PROCESSING ~ HIGH HYDROSTATIC PRESSURE Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 17 07/06/2011 IPN-Dept ITP/IPB Historical Timeline 1895 H. Royer uses high pressure to kill bacteria. 1899 Bert H. Hite at the West Virginia Agricultural Experimental Station examined pressure effects on milk, meat, t fruits f it and d vegetables. t bl 1914 P. W. Bridgman coagulated egg albumen under high pressure. 1990 First commercial products like fruit juices, jams, fruit toppings and tenderized meats introduced in Japan. 1995 Orange juice commercialized in France. 1997 Market introduction of guacamole in the US and sliced cooked ham in Spain. 1999 Oysters introduced in the US. 2000 Range of salsas launched in the US market. Purwiyatno Hariyadi/ITP530 High hydrostatic pressure • Foods "pasteurized" pasteurized by HHP undergo pressures of up to 80,000 psi at or near ambient temperatures (under 45°C). • Under these conditions, HHP is effective in inactivating most vegetative pathogens commonly found in the foods. • Commercially available HHP-processed products in E Europe and dA Asia i iinclude l d jjuices, i jjams, jjellies, lli meatt and yogurts. • Consumers in this country can buy HPP-processed guacamole and oysters. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 18 07/06/2011 IPN-Dept ITP/IPB High hydrostatic pressure • There is significant commercial interest in development of other products. • Food processed by HHP reportedly has better retention of flavor, texture, color, and nutrients. • The processing cost is slightly higher (two to three cents per pound) than for conventional processes processes. Purwiyatno Hariyadi/ITP530 How High ?? Two elephants balanced on a piston with a cross section of a dime will create a pressure of 400 Mega Pascal (Mpa). This is approximately 60,000 pounds per square inch. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 19 07/06/2011 IPN-Dept ITP/IPB HHP processing • High Pressure can kill microorganisms by interrupting with their cellular function without the use of heat that can damage the taste, texture, and nutritional value of the food. Purwiyatno Hariyadi/ITP530 HHP processing • The "mechanism" of high-pressure based bacteria kill is low energy and dd does nott promote t the th fformation ti off new chemical h i l compounds, "radiolytic" by-products, or free-radicals. • Vitamins, texture and flavor are basically unchanged. • For example, enzymes can remain active in high pressure produced orange juice. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 20 07/06/2011 IPN-Dept ITP/IPB HHP system • This 35-liter high-pressure b t h unitit ffrom Fl batch Flow International can process up to 700 lbs. an hour and is designed for prepackaged products such as bottled juices. • Units with capacities p up p to 300 liters are available. • Food scientists are optimistic about hydrostatic pressure's ability to extend shelf life and produce safe food. Purwiyatno Hariyadi/ITP530 HHP system Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 21 07/06/2011 IPN-Dept ITP/IPB Industrial High Pressure Food Processors Complete systems are offered for bulk and in container processes. Purwiyatno Hariyadi/ITP530 Applications for High Pressure Processing are found in the areas of... P Preservation ti Elimination or substantial reduction of spoilage microorganisms and enzymes for shelf life extension of refrigerated food products with superior sensory quality, e.g. juices, jams, guacamole, salsa, meat & dairy products, seafood (commercialized) Acidified and low-acid shelf-stable products (under development) Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 22 07/06/2011 IPN-Dept ITP/IPB Applications for High Pressure Processing are found in the areas of... Food safety Elimination of pathogens: e.g. Listeria in meat products, Salmonella in eggs and poultry, Vibrio in oysters Hypotheses for vegetative cell inactivation... - denaturation of proteins and enzymes - damage of DNA replication & transcription - solidification of membrane (phospho)lipids - breakage of bio-membranes (cell leakage) Spores are very resistant to pressure, but can be destroyed by combining pressure with elevated temperatures Purwiyatno Hariyadi/ITP530 Applications for High Pressure Processing are found in the areas of... Fruit Juice treated with HHP • Juice tests have shown that food pathogens such as salmonella and E.coli 0157:H7 can be effectively destroyed without changing the fruit juice's fresh, natural characteristics. • A pressure exposure of 80,000 psi for 30 seconds can achieve a 3-5 log reduction of all of the pathogens of concern in fresh juice Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 23 07/06/2011 IPN-Dept ITP/IPB Applications for High Pressure Processing are found in the areas of... Oyster treated by HHP • Another example p of food safety y is the destruction of Vibrio bacteria in raw oysters without destroying the raw feel and taste of the oyster. • A pressure of 200 to 300 MPa for 5 to 15 minutes at 25C inactivated : · Vibrio parahaemolyticus ATCC 17803, · Vibrio vulnificus ATCC 27562, · Vibrio choleare ATCC 14035, · Vibrio choleare non-O:1 ATCC 14547, · Vibrio hollisae ATCC 33564 · Vibrio mimicus ATCC 33653 (from: "D. Berlin, D. Herson, D. Hicks, and D. Hoover; Applied and Environmental Microbiology, June 1999“) Purwiyatno Hariyadi/ITP530 Applications for High Pressure Processing are found in the areas of... Oyster treated by HHP Pressure shucked raw clams. Pressure not only destroys the vibrio family of bacteria that can be found in shellfish, but also detaches the meat from the shell, saving labor gp production and increasing efficiency. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 24 07/06/2011 IPN-Dept ITP/IPB Applications for High Pressure Processing are found in the areas of... Styrofoam cup subjected to 40,000 psi, fruit pack (with juice) and sliced ham subjected to 80,000 psi. Purwiyatno Hariyadi/ITP530 Commercial High-Pressure Processed products marketed in Japan, Europe and the United States Guacamole & Salsas Avomex (Keller, TX) Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 25 07/06/2011 IPN-Dept ITP/IPB Commercial High-Pressure Processed products marketed in Japan, Europe and the United States Purwiyatno Hariyadi/ITP530 Commercial High-Pressure Processed products marketed in Japan, Europe and the United States Jams & Fruit Toppings (Japan) Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 26 07/06/2011 IPN-Dept ITP/IPB Examples of products commercialised in Europe and treated on HYPERBAR installations supplied by ACB ULTI / PAMPRYL (Groupe PERNOD-RICARD) - France freshey squeezed fruit juice Fresh pressed ESPUNA - Spain – sliced cooked ham Purwiyatno Hariyadi/ITP530 Applications for High Pressure Processing are found in the areas of... Texturization As an alternative to heat processing texturization can be accomplished by exposing protein (e.g. egg, whey, soy) and hydrocolloid (e.g. pectin, starch) solutions to hydrostatic pressure. The resulting gels are characterized by uniquely different textures. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 27 07/06/2011 IPN-Dept ITP/IPB Applications for High Pressure Processing are found in the areas of... H t Heat-sensitive iti compounds d HPP offers the unique potential to stabilize products with heat-sensitive components (e.g. flavors, nutrients, biologically active compounds). Biotechnology Specific enzymes can be activated under pressure leading to enhanced reaction rates and shorter process times. Purwiyatno Hariyadi/ITP530 Thermally-assisted high-pressure lifts quality of shelf-stable foods Process Variables for Optimum Quality Pressure Products 90°C 700 MPa Main meal entrees, meats, pasta dishes, most vegetables, sauces, cheese, soups, stews, flavored milk drinks 80°C 830 MPa Whole potatoes, most vegetables 70°C 1,000 MPa All potato products, all vegetables, seafood 60°C 1,240 MPa Eggs, milk Temperat ture Source: Richard S. Meyer, PhD, Washington Farms, Inc. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 28 07/06/2011 IPN-Dept ITP/IPB Effect of pressure is very similar to the effect of temperature in thermal processes Figure. Change in inactivation of Zygosaccharomyces bailii with pressure. Note that 345 MPa = 50,000 psi. (Enrique Palou, GRA, BSysE Dept., WSU) Purwiyatno Hariyadi/ITP530 Thermally-assisted high-pressure lifts quality of shelf-stable foods What's needed to commercialize UHP for sterilizing shelf-stable products? Two things must be done. First, develop the kinetic information necessary to file a petition with the FDA and USDA. To do that, we have to select the most heat and pressure-resistant strain of Clostridium botulinum. That work is underway with the Dual Use Science & Technology (DUST) Program 2000 headed by Dr. Patrick Dunne at the U. S. Army Natick Soldier Center in Natick, Mass. Dual use refers to developing the technology for both military and industrial applications. Second, we need commercial-size, inexpensive high-pressure vessels. Flow International Corp. (Kent, Wash.) is nearest to delivering that right now. They have a 215-liter vessel which is very close to all the critical parameters for delivering a sterile product. They're leading with the technology, and just need to build a vessel to order. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 29 07/06/2011 IPN-Dept ITP/IPB Dosis Irradiasi • Dikendalikan oleh “the International Commission on Radiological Units” Rad • 1 Rad = jumlah irradiasi yang menyebabkan 1 g bahan yang diirradiasi akan menyerap energi sebesar 100 erg (1 Rad = 100 erg/g) • Satuan umum • 1 Grey = jumlah irradiasi yang menyebabkan 1 kg bahan yang diirradiasi akan menyerap energi sebesar 1 joule; • 1,000 , Grey y = 1 kGy. y • 100 Rad = 1 Gy “Grey“ Purwiyatno Hariyadi/ITP530 Dosis Irradiasi Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 30 07/06/2011 IPN-Dept ITP/IPB PENGARUH IRRADIASI PADA MIKROBA D10,veg bacterium D10,yeast D10 : dosis irradiasi yang menyebabkan pengurangan jumlah mikroba dengan faktor 10 D10 dose Purwiyatno Hariyadi/ITP530 PENGARUH IRRADIASI PADA MIKROBA Organism Dvalue Resistance Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 31 07/06/2011 IPN-Dept ITP/IPB PENGARUH IRRADIASI PADA MIKROBA _______________________________________________________ organisme medium in D10 (Rad) which irradiated _______________________________________________________ Bacterial spore C botulinum A36 buffer 0.33 106 B53 buffer 0.33 106 B57 canned chicken 0.37 106 C. sprogenes 367 water 0.22 106 Veg bacteria Sal typhimrium E coli li buffer egg yolk b ff buffer 2.0 104 8.0 104 9 0 103 9.0 Yeast Sacc cerevisiae saline 5.0 104 Mold Asp niger saline 4.7 104 Purwiyatno Hariyadi/ITP530 PENGARUH IRRADIASI PADA MIKROBA Model inaktivasi mikroba oleh irradiasi bisa juga dinyatakan sbb : N = N0 e − D Dn dimana, dimana, N = jumlah mo setelah iradiasi iradiasi,, = jumlah mo awal (sebelum iradiasi iradiasi), ), N0 D = Dosis radiasi yang diterima diterima,, dan Dn = konstanta k konstanta; t t ; tgt t t jjenis i mo dan d kondisi k di i lingkungan li k Konstanta Dn = dosis radiasi yang menyebabkan kematian mikroba sebanyak 63% populasi populasi.. Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 32 07/06/2011 IPN-Dept ITP/IPB APLIKASI TEKNOLOGI IRRADIASI PADA BAHAN PANGAN Irradiasi dosis rendah (sampai 1 kGy) Menghambat perkecambahan (1 Gy = 1 Joule/Kg) Menunda pematangan buah Dosis medium (1-10 kGy) Mengurangi risiko patogen dan meningkatkan masa simpan (keawetan) Dosis tinggi ( > 10 kGy) Strilisasi Purwiyatno Hariyadi/ITP530 APLIKASI TEKNOLOGI IRRADIASI PADA BAHAN PANGAN Purwiyatno Hariyadi/ITP530 Purwiyatno Hariyadi/IITP530- 2011 33