[ CARE AND USE MANUAL ]
30 mm ID Preparative Guard Cartridges and Guard Holders
CONT ENT S
I.
INT RODUCTION
II.
SPECIFICATIONS
III.
TIGHT ENING PROCEDURE
IV.
START-UP RECOMMENDATIONS
a.Background (Avoid Pressure Pikes)
b. Start-Up
V.
INSTALLING AND REPLACING
THE GUARD CART RIDGE AND
CART RIDGE HOLDER
VI.
WHEN TO REPLACE THE
GUARD CART RIDGE
VII.
REPLACING THE O-RINGS
VIII.
T ROUBLESHOOTING
IX.
GUARD CART RIDGE STORAGE
a.HPLC Guard Cartridges
b. SFC Guard Cartridges
X.
ORDERING INFORMATION
XI.
WARRANT Y/SERVICE INFORMATION
I. INT RODUCTION
Thank you for purchasing the 30 mm ID Preparative Guard Cartridge and
Guard Holder assembly. This preparative guard assembly is designed to
protect 30 mm ID Preparative Optimum Bed Density (OBD™) Columns and
is suitable for both HPLC and SFC.
II.SPECIFICATIONS
Preparative Guard Cartridge Specifications
Dimension
30 mm ID x 10 mm length
Pressure Limit
6,000 psi (413 bar)
Materials Construction
316 stainless steel
Solvent Compatibility
Suitable for common HPLC (reversed-phase and
normal-phase) and SFC mobile phases. Minor
swelling of O-rings in THF and chloroform may
require more frequent replacement if leaks
observed. Solvent compatibility of the packing
is the same as that of the preparative column.
Column Volume
7 mL
[ CARE AND USE MANUAL ]
III. TIGHT ENING PROCEDURE
1. After inserting guard cartridge, tighten holder by hand
until O-rings make contact with the guard cartridge.
2. While holding the 2-1/16” flats, use a 3/4” wrench to
tighten holder cap until O-rings are fully seated, between
5-9 tick marks.
V.INSTALLING AND REPLACING THE GUARD CART RIDGE AND CART RIDGE HOLDER
Install the cartridge holder onto the HPLC system in front of the
column using the rigid connector. The tubing in the rigid connector is
0.020” or 0.5 mm ID. For all other connections, use the same tubing.
Installing and replacing the guard cartridge:
1. Remove the cartridge holder from the system.
IV. START-UP RECOMMENDATIONS
a. Background (Avoid Pressure Pikes)
It is possible that a SUDDEN high flow rate start at 50-60 mL/min
may contribute to initial disruption of the prep guard cartridge
bed leading to the peak splitting and shortened lifetimes.
It is recommended to start the flow rate up MORE SLOWLY
(over 5 or 10 minutes) to avoid this issue.
b. Start-Up
1. Purge the pumping system of any buffer-containing mobile
phases and connect the inlet end of the column to the injector
outlet.
2. Flush column with 100% organic mobile phase (methanol or
acetonitrile) by setting the pump flow rate to 0.1 mL/min and
increase the flow rate to 50-60 mL/min over 5 minutes.
3. When the mobile phase is flowing freely from the column
outlet, stop the flow and attach the column outlet to the
detector. This prevents entry of air into the detection system
and gives more rapid baseline equilibration.
4. Gradually increase the flow rate as described in step 2.
5. Once a steady backpressure and baseline have been achieved,
equilibrate the column with a minimum of 10-column volumes
of the mobile phase.
6. For added assurance, perform an efficiency test on the prep
guard cartridge before using it. Waters recommends using
a suitable solute mixture, as found in the “Performance Test
Chromatogram”, to analyze the prep guard cartridge upon
receipt. Determine the number of theoretical plates (N) and
use this value for periodic comparisons.
2
30 mm ID Preparative Guard Cartridges and Guard Holders
2. Unscrew the holder cap from the holder body.
3. Remove the old guard cartridge, if present.
4. Visually inspect the O-rings for damage or debris.
Replace, if necessary (see Section V).
5. Install the new guard cartridge. The direction of the flow
arrow on the guard cartridge and holder body should point
towards the detector.
6. Re-assemble the holder and tighten according
to instructions above.
7. Attach inlet side of assembled cartridge holder to the tubing
from HPLC injector.
8. Flush with 10 volumes of mobile phase at a low flow rate.
9. Attach outlet side of the cartridge holder to the column
inlet using rigid connector.
10. Check for leaks.
Guard Holder Body
Tick Marks
3/4” Wrench
2-1/16” Flats
Guard Cartridge
Guard Holder Cap
Figure 1. Preparative Guard Cartridge.
[ CARE AND USE MANUAL ]
VI. WHEN TO REPLACE THE GUARD CART RIDGE
VII. REPLACING THE O-RINGS
Replace guard cartridges before contaminants affect your
separation. For best results, replace the guard cartridge when:
The O-rings should be replaced if leaks from the cartridge
holder are observed. You will need replacement part number
186007012 (O-rings - 30 mm ID Prep Guard Holder, 2/pk),
a pointed device (such as a needle or dental pick), and a clear
workspace where small parts are easily retrieved if dropped.
1. The quality of your separation deteriorates.
2. The initial backpressure increases significantly.
(more than 20 percent).
3. Contaminant peaks appear in the chromatogram.
To replace the O-rings:
1. Remove the cartridge holder from the system.
2. Unscrew the holder cap from the holder body.
3. Remove the guard cartridge.
4. Using a sharp needle, pry the O-rings from their grooves in
the holder cap and body. Be careful not to scratch the groove,
this may lead to leaks. It may be helpful to insert the needle
partway into the O-ring in order to remove it.
5. Examine the grooves and new O-rings. Remove any particulate
matter with a cotton tip applicator.
Weep Hole
Figure 2. Weep Hole on the Side of the Prep Guard Holder.
6. Install the new O-rings into the grooves. To ensure O-rings are
installed properly in the groove, insert guard cartridge into
holder body or holder cap and rotate 1/4 turn, applying light
hand pressure against the cartridge.
7. Re-assemble holder with cartridge and tighten according to
directions above.
O-ring
Needle or Dental Pick
Figure 3. Prep Guard Cartridge O-ring, and Needle/Dental Pick.
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30 mm ID Preparative Guard Cartridges and Guard Holders
[ CARE AND USE MANUAL ]
VIII.T ROUBLESHOOTING
Troubleshooting Tips
Symptom
Cause
Corrective Action
Cartridge not seated in end fitting
Disassemble, properly seat cartridge
Solvent leak at seam Particles on the face of the
of cartridge weep hole O-ring or on metal seat
Broad peaks, loss
of peak resolution
High backpressure
Wipe clean with a cotton tip applicator
Worn O-ring
Replace O-ring
Packing material fouled or voided
Replace with new guard cartridge
Guard cartridge packing material
different from prep column packing
Replace with matching guard cartridge
Filters in guard cartridge
plugged or fouled
Replace with new guard cartridge
Guard cartridge chemically fouled
Replace with new guard cartridge
IX.GUARD CARTIDGE STORAGE
a. HPLC Guard Cartridges
To maintain the integrity of the bonded phase as well as the guard cartridge hardware and frits, flush
the guard cartridge with at least eight column volumes of 100% acetonitrile after every use. Never
store the guard cartridge in methanol or aqueous methanol. If the mobile phase contains a buffer salt,
first flush the guard cartridge with at least ten column volumes of 18 MegaOhm water followed by at
least eight column volumes of 100% acetonitrile. Please refer to Section II for the column volume.
b. SFC Guard Cartridges
Purging the SFC prep guard cartridge in pure CO2 is recommended prior to storage. This will eliminate
or minimize retention changes of the guard cartridge during storage. Storing the guard cartridge in
methanol is not recommended.
4
30 mm ID Preparative Guard Cartridges and Guard Holders
[ CARE AND USE MANUAL ]
X.ORDERING INFORMATION
Preparative Guard Cartridge Holder, O-rings and Connector
Description
Part Number
Prep Guard Holder, 30 x 10 mm
186006912
O-rings, 30 mm (2/pk)
186007012
Rigid Connector Assembly, 0.020"
405000981
XI. WARRANT Y/SERVICE INFORMATION
Waters Corporation warrants its guard cartridges and guard holders in accordance with the
following terms and conditions:
Waters will replace without cost, any guard cartridge or guard holder that fails to perform
satisfactorily, if notified within 90 days from your receipt. To return guard cartridges and
guard holders, the user must have a Return Authorization Number granted by the Waters
Technical Service Department.
Approval is subject to the following exclusions:
1. Physical damage to the guard cartridge or guard holder because of misuse or abuse.
2. Damage to the guard cartridge or guard holder because of use with incompatible
solvents or use at incorrect temperatures or pressures.
3. Failure or decreased performance as a consequence of normal use.
For more information, please contact your local Waters representative.
Waters is a registered trademark of Waters Corporation. OBD
and T he Science of W hat’s Possible are trademarks of Waters
Corporation. All other trademarks are the property of their
respective owners.
©2013 Waters Corporation. Produced in the U.S.A.
September 2013 720004671EN Rev. B K P-PDF
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com