The Best
Connections are
Made to Last
Simplify Your
GC Connections
SilTite® GC Connections
Column connections in the GC
have traditionally suffered from
unreliability, leaks, excessive dead
volumes and lack of inertness,
leading to poor chromatography
results and instrument down time.
SGE Analytical Science SilTite®
GC Connections are designed to
minimize installation time and
provide ongoing, robust connections
throughout the life of the GC column.
•Easy to install and leak-free
• Superb operational stability
•Low dead volume
Using SilTite GC Connections means
less time on maintenance, and more
time to focus on your analysis!
• Chemically inert
Injection Port
Issues
•Having to retighten Graphite Vespel ferrules due to thermal cycling of the oven.
•Difficulty setting the GC column at the same depth into the injection port liner each
and every time.
•Introduction of graphite particles into the system and maintaining system cleanliness.
•Leaks introduced due to porous sealing materials.
•Needing to use wrenches to create good seals.
®
SilTite FingerTite INJ
Solutions
The SilTite FingerTite ferrule system eliminates the need for wrenches. The FingerTite
nut is designed to seal under finger force, can never be over-tightened or break the
GC column.
leakfree
inert
seal
Permanent
Leak-free
Sealing on the
GC Column
Clean and
Inert Surfaces
Optimum
Sealing for
MS Systems
SilTite
SilTite Metal Ferrules | Agilent Technologies
Instrument
The setting tool included in the starter kit enables pre-setting of the ferrule onto the
GC column outside of the GC oven, ensuring repeatable setting of the column relative
to the injection port liner every time.
Column ID
0.1-0.25 mm
0.32 mm
0.53 mm
0.1-0.25 mm
0.32 mm
0.45-0.53 mm
1/32"
For GC-MS Interface Connections (Starter Kit)
For Split / Splitless Injectors (Starter Kit)
Pack
Size
10*
10*
10*
10 #
10 #
10 #
10 #
Ferrule ID
0.4 mm
0.5 mm
0.8 mm
0.4 mm
0.5 mm
0.8 mm
0.81 mm
Part No.
073200
073201
073202
073270
073271
073272
073273
Replacement
Ferrules (Pk 10)
073220
073221
073222
073220
073221
073222
073219
* Includes ten SilTite ferrules, two SilTite nuts. # Includes ten SilTite ferrules, two SilTite nuts and two SilTite inlet base seals.
Injection Port
Detector
Detector
SilTite FingerTite FID
seal
•High background noise and unreliable results.
•Instrument downtime.
Solutions
GC Column Connectors
Use SilTite and SilTite FingerTite ferrules as your
detector connections.
no
absorption
Issues
SilTite
SilTite FingerTite MS
Permanent
Metal-on-metal
Seal Which is
Leak-free
(Vital for
MS Detectors)
No Absorption
of Analytes or
Column Creep
inert
Clean and
Inert Surfaces
Peak Tailing
Q. Our peaks are tailing, how can we avoid this?
Low Response
A. There are several possible causes, incorrect column
installation (remove the first meter of the column; then
re-install the column making sure that it is held securely
by the ferrule, and recheck it), the liner may be active
(replace the liner with a clean, deactivated liner), the
column may be active (try a new column), or it may be
the wrong type column for the analysis (the phase film
thickness may be too low, or a particular type of phase
may be required).
Split Peaks
A.The column or liner may be active, remove the first
meter of column; recheck; and replace column if
necessary. Replace the liner with a clean, deactivated
liner. The detector gas flows may not be correct,
check and re-adjust them if necessary. Make sure
that the standards and samples concentrations have
been calculated correctly, and that the standards and
samples have been diluted correctly. Check for leaks at
both the injector and the detector end of the column,
and re-tighten all connections if necessary.
Q. We have split peaks, what can we do?
A. This could be caused by a poorly cut column inlet, or a
small fragment of graphite ferrule lodged in the inlet of
the column (remove the first meter of the column; then
re-install the column and recheck it). Other possible
causes are poor injection technique, more than one
solvent being injected, or by a lack of resolution. Refine
your injection technique and use only one injection
solvent. If you suspect that there is a lack of resolution,
change the temperature program, try a longer column,
one with a smaller inside diameter, or a different phase.
Q.We are getting a lower than expected response
(peak area) for specific peaks. What could be
causing this?
High Bleed
Q.We are seeing high column bleed. What should be
we check?
A.Check published maximum temperature of the column
and make sure that this hasn’t been exceeded. Check
the carrier flow rates / velocity, make sure that they
are correct for column inside diameter and length and
adjust them if necessary. Make sure that the column has
not moved in detector. Is the ferrule holding it securely?
Check for leaks, and re-tighten all connections if
necessary. Check that the oxygen filter is not spent
– replace it if necessary. Make sure that the detector
temperature is higher than final column temperature
if possible. Check cleanliness of detector and clean it if
necessary. Cut 50 cm from the front end of the column
and recondition column.
Relative Abundances:
28/69 = 9.08 Nitrogen%
32/69 = 2.56 Oxygen%
% Response of Base Fragment 69 m/z
Expert Tips
% Response of Base Fragment 69 m/z
Leak-free Seal Using SilTite Compared to a Graphite Vespel® Ferrule
100
20
Air
10
0
10
15
20
25
30
35
40
45
50
55
60
65
70
75
Relative Abundances:
28/69 = 0.97 Nitrogen%
32/69 = 0.39 Oxygen%
SilTite FingerTite Ferrules | Agilent Technologies
100
20
No Air
10
0
Figure 1. MS trace using a Graphite Vespel® ferrule after five
temperature cycles.
10
15
20
25
30
35
40
45
50
55
60
65
Part No.
0.1-0.25 mm
0.4 mm
§
073610
0.1-0.25 mm
0.4 mm
§
073612
SilTite FingerTite Agilent INJ / FID Kit
0.53 mm
0.7 mm
§
SilTite FingerTite Agilent Injector Kit
0.53 mm
0.7 mm
SilTite FingerTite Agilent Capillary / FID Kit
0.53 mm
SilTite FingerTite Agilent Capillary Injector Kit
0.53 mm
SilTite FingerTite Agilent INJ / FID Kit
SilTite FingerTite Agilent INJ / MS Kit
Pack
Size
Part No.
SilTite FingerTite Agilent Capillary Adaptor
1
0736101
SilTite FingerTite Agilent MS Adaptor
1
0736102
07361053
SilTite FingerTite Agilent FID Detector
1
0736103
§
07361054
SilTite FingerTite Agilent Injector
1
0736104
0.7 mm
§
07361153
0.7 mm
§
07361154
Description
§ Each starter kit includes all the parts necessary to convert one GC system (one injector and one detector) to the SilTite FingerTite system suitable for installing 0.1-0.25 mm ID capillary columns.
In addition there are five SilTite FingerTite nuts, ten 0.4 mm ID SilTite FingerTite ferrules, and a ferrule install tool which allows you to seat the ferrule in the correct position on the capillary column.
GC Column Connectors
40 ºC
Issues
•Poor connectors joining retention gaps, transfer lines or a second dimensional column
to an analytical column are a potential source for band broadening and activity.
•Popular glass press-fit connectors are prone to leaking, especially at high temperatures.
•Conventional unions leak during column temperature programming.
•The presence of dead volume in GC column connectors contributes to peak tailing.
leakfree
SilTite µ-Union
5 min
Use the SilTite µ-Union. Its small dimensions provide significant advantages.
Dichlorvos; 109.0 (50.0:350.0);
pest1ppm q-connect001.xms; Filtered
Dichlorvos; 109.0 (50.0:350.0);
pest1ppm µ-Union002.xms; Filtered
10
0
6.5
6.6
6.7
5 ºC
no
thermal
lag
No Thermal
Lag Effects that
are Associated
with Larger
Connectors
SilTite µ-Union
Peak Shape Using μ-Union - No Loss of Resolution
20
5 ºC
Leak-free Use
with Column
Temperature
Programming
Solutions
6.4
40 ºC
5 min
30
6.8
Minutes
Approved Distribution Partner
Pack
Size
Column ID
75
Figure 2. MS trace using a SilTite ferrule after five temperature
cycles. Using an MS, no leaks can be detected, even after
400 temperature cycles between 70 ºC and 400 ºC.
40
For more information visit
www.sge.com/gcconnections
or contact techsupport@sge.com.
70
Ferrule ID
Description
Figure 3. Evaluation of SilTite μ-Union compared to glass press-fit connectors on pesticide analysis.
Pack
Size
Part No.
Replacement
Ferrules (Pk 10)
Replacement Nuts
(Pk 5)
0.1-0.25 mm
10
073560
073470
073553
0.32 mm
10
073561
073470
073553
0.1-0.25 mm
0.53 mm
10
073562
073470
073553
0.32 mm
0.32 mm
10
073563
073471
073553
0.32 mm
0.53 mm
10
073564
073471
073553
0.53 mm
0.53 mm
10
073565
073472
073553
Column 1 ID
Column 2 ID
0.1-0.25 mm
0.1-0.25 mm
No GC Column
‘Cold Spots’