PRODUCT GUIDE
GeneArt PerfectMatch TALs
®
GeneArt PerfectMatch TALs
®
Precise and extraordinarily flexible genome editing
GeneArt PerfectMatch TALs provide custom DNAbinding proteins designed for accurate DNA targeting
and precise genome editing. Unlike other technologies
that limit the choice of targets, or provide ambiguous
results, GeneArt PerfectMatch TALs help enable
the targeting of any locus in the genome. Previous
versions of GeneArt Precision TALs required a thymine
nucleotide (T) at the 5´ end of each target sequence. The
5´ T constraint limits the flexibility of TAL effector target
sites in the genome and prevents some specific sites
in the genome from being targeted [1]. With our new
GeneArt PerfectMatch TALs, the 5´ T requirement is
removed, making it possible to design a TAL effector pair
for anywhere in the genome, giving you broad flexibility
in target design. In addition, Thermo Fisher Scientific
has recently secured rights to certain intellectual
property around TALs, clarifying a path for you to move
confidently forward and innovate.
®
®
®
®
Product details
Transcription activator-like (TAL) effectors are a
widely used technology for precise and efficient gene
editing in living cells. Native TAL effectors are DNAbinding proteins produced by plant pathogens of
the genus Xanthomonas. When these bacteria infect
plants, the TAL effector proteins bind to selected
regulatory DNA sequences and directly modulate host
gene expression [2]. The DNA-binding domain of TAL
effectors consists of a variable number of amino acid
repeats, called repeat-variable di-residues (RVDs),
which recognize a single DNA base pair. We provide
GeneArt TALs with special functional domains
(nuclease, activator, or repressor domains) that are
designed to interact with unique DNA targets in large,
complex genomes.
®
Before now, target sites for customized TAL effectors
required a 5´ T in the target sequences for maximal
binding activity [3–5] (Figure 1). The 5´ T constraint
limited the flexibility of TAL effector target sites in the
genome and prevented some specific sites in the genome
from being targeted. Structure studies suggested the
N-terminal domain (NTD) of the TAL effectors, not
the central repeat domains, was responsible for the
interaction with the 5´ T of the target [6]. We developed
our second-generation TAL effectors, GeneArt
PerfectMatch TALs, by mutating the N-terminal domain
to relax its specificity for 5´ T. GeneArt PerfectMatch
TALs can target DNA sequences with any 5´ base (T, G,
C, or A) (Figure 1), with performance equal to or better
than that of GeneArt Precision TALs.
®
®
GeneArt PerfectMatch TALs are derived from GeneArt
Precision TALs and contain a truncated TAL effector
fused to a FokI nuclease domain. The mutation converts
the 5´ T binding motif at the TAL effector N-terminus to a
universal binding motif (it will bind to any base: A, G, C, or
T). With the mutation, GeneArt PerfectMatch TALs can
be designed to precisely bind to any sequence across the
genome in a sequence-specific manner.
®
®
®
GeneArt PerfectMatch TALs are provided as lyophilized
DNA in two kinds of vectors: a Gateway adapted entry
vector and a CMV expression vector (Figures 2 and 3).
®
®
attL2
okI
mF
t- er
Ka
na
®
cin
my
®
N-TAL FokI entry vector
4,349 bp
eat
RV
D
TAL
N
-t e r m V 5
att L
DNA-binding domain
Functional
domain
FokI
13–18 bp
TNNNNNNNNNNNNNNNNNN
1
Figure 2. Gateway adapted entry vector. Gateway adapted entry vectors
allow easy transfer through an LR recombination reaction to destination
vectors designed to facilitate high-level expression of the TAL effectors in
your cells of choice.
®
A
pUC origin
18 or 24 rep
GeneArt TALs engineered with the FokI nuclease can
be used for targeting specific genes for editing (silencing,
incorporation of exogenous DNA, or mutation). FokI is a
type IIS restriction endonuclease from Flavobacterium
okeanokoites, consisting of an N-terminal DNA-binding
domain and a nonspecific DNA-cleavage domain at the
C-terminus. A FokI nuclease pair binds to duplex DNA at
the target sites designated by the DNA-binding domains,
with each member of the pair cleaving one strand of the
DNA target.
TA
LC
®
®
NNNNNNNNNNNNNNNNNNT
pU
FokI
15–16 bp
NNNNNNNNNNNNNNNNNNN
18 or 24 rep
N-TAL FokI CMV
expression vector
NNNNNNNNNNNNNNNNNNN
FokI
cillin®
Functional
domain
BGHpA
5,239 bp
A m pi
DNA-binding domain
I
Fok
m
r
-te
in
rig
Co
B
TA
LC
FokI
eat
RV
D
Figure 1. Designing target sites for customized TAL effectors for
maximal binding. (A) GeneArt Precision TALs encode a DNA-binding
protein specific to a customer-submitted sequence, fused to a FokI
nuclease domain for genome editing. The sequence targeted by our
first-generation TAL effectors must have a T at its 5´ end, and spacing
between forward and reverse TALs must be 13–18 bp for proper pairing of
the FokI nucleases and creation of a double-stranded break. (B) GeneArt
PerfectMatch TALs eliminate the 5´ T constraint of GeneArt Precision
TALs. GeneArt PerfectMatch TALs allow targeting of any sequences
across the genome; 15–16 bp spacing between the two TAL effector
targets is optimal for GeneArt PerfectMatch TALs.
®
®
®
®
M
V
®
TAL
N
PC
-te r m V 5
Figure 3. CMV expression vector. This vector contains a CMV promoter,
which drives high-level expression of the TAL effector in mammalian
systems. GeneArt PerfectMatch TALs provided in this vector can be
directly used in mammalian systems without extra subcloning.
Optional: A GeneArt PerfectMatch TAL cassette can be transferred into
your favorite expression vector with restriction enzymes NotI and HindIII.
®
®
Identify your target
gene of interest
Choose vector
Place order
Transfect
Analyze
Figure 4. Workflow for GeneArt PerfectMatch TALs. The GeneArt PerfectMatch TALs workflow is similar to the GeneArt Precision TALs workflow.
®
®
®
Workflow
The workflow for GeneArt PerfectMatch TALs is similar to that of the GeneArt Precision TALs (Figure 4).
®
®
Step 1: Identify your target gene of interest. Design the targeting sequences for GeneArt PerfectMatch TALs
yourself, or contact GeneArt support (GeneArtSupport@lifetech.com) for free design consultation.
®
®
GeneArt PerfectMatch TAL binding site rules:
®
• GeneArt PerfectMatch TAL offerings allow the construction of TAL functional proteins directed to either 18- or
24-base DNA target sites.
®
• Each target site must be preceded by a 5´ “N” when you place the order, because the N-terminus of the
GeneArt PerfectMatch TAL protein binds to any DNA base. The letter N represents any base—A, G, C, or T. The
5´ N does not count as one of the 18 or 24 bases to be selected for targeting your specific site. (This differs from
GeneArt Precision TALs.)
®
®
• Nuclease pairs need to be designed with a spacing of 13–18 bp between the target sites on opposite strands of
the DNA. However, we recommend a spacing of 15–16 bp between the target sites to achieve maximal nuclease
activity. The target sites can be either 18 or 24 bp in length. Figure 1B should be used as a reference for the
orientation of the binding domains. (This differs from GeneArt Precision TALs.)
®
• The contribution of individual binding motifs within the DNA-binding domain to TAL effector binding efficiency
is thought to differ, because strong and weak binding motifs exist. The A- and T-binding motifs are thought to
fall within the “weak binder” category, whereas the C- and G-binding motifs are thought to be “strong binders”.
Stretches of more than 5 weak binders should be avoided at the extreme 5´ end of the binding domain (not
counting the 5´ “N”), or if they are not flanked by Cs. TAL effectors with DNA-binding domains composed of
mixed binding motifs generally give the best results.
• In the context of the living cell, DNA accessibility also determines TAL effector efficiency. Chromatin, DNA
methylation, and/or proteins bound to the DNA may interfere with TAL effector binding.
Step 2: Choose the vector to be received (Gateway adapted entry vector or CMV expression vector).
®
Step 3: Place your order by downloading and completing the order form and emailing it to
geneartsupport@lifetech.com. You will receive a clone with a verified, optimized sequence
approximately 2 weeks after confirming your order.
Step 4: Create an expression clone (optional). To create an expression clone, perform the LR recombination
reaction to transfer the gene of interest into your destination vector of choice. If you chose the CMV expression
vector in step 2, please directly go to step 5. If you chose the Gateway adapted entry vector in step 2, you need to
complete this step.
®
Step 5: Transfect.
Step 6: Analyze the sample for gene modification 72 hours post-transfection using the GeneArt Genomic
Cleavage Detection Kit (Cat. No. A24372).
®
Figure 5
Analysis: data and applications
Double-stranded DNA breaks can be created at your
specified genomic locus by using a pair of GeneArt
PerfectMatch TAL proteins that have been fused to
the FokI endonuclease (see Figure 1B). Using a pair
of TAL proteins for the targeting reduces off-target
effects. The breaks induced by the FokI nuclease
domain are subsequently repaired through either of
two endogenous cellular mechanisms: nonhomologous
end joining (NHEJ), or homology-directed repair
(HDR). NHEJ is prone to errors and often introduces a
frameshift mutation when it occurs within the coding
sequence of a protein-coding gene, effectively silencing
the gene. Homologous DNA “donor sequences” can
be used with HDR to introduce a defined new DNA
sequence. Consequently, GeneArt TAL proteins fused
to FokI endonucleases can be used to induce gene
silencing or to accurately insert an engineered DNA
fragment into an exact location in the genome. The
effectiveness of all genome-editing tools such as
CRISPRs, TAL effectors, and zinc fingers depends on
many factors, including the footprint of target sites
(DNA sequences), spacing distance, DNA accessibility,
cell type and condition, and transfection efficiency.
Therefore, it is necessary to determine the cleavage
efficiency prior to continuing with labor-intensive
and expensive experiments. The GeneArt Genomic
Cleavage Detection Kit (Cat. No. A24372) provides a
simple, reliable, and rapid method to determine the
nuclease cleavage efficiency at a given locus.
HPRT
®
®
®
To determine the effectiveness of GeneArt PerfectMatch
TALs, we have compared the genome cleavage
efficiencies of GeneArt PerfectMatch TALs to those
of GeneArt Precision TALs at specific loci in a broad
range of cell types. Target sequences for GeneArt
PerfectMatch TALs and GeneArt Precision TALs were
designed to be close to each other (within a few bases).
In Figure 5, all TAL effector targets at the HPRT locus are
shown as an example. Note that all TAL effectors tested
in this comparison contain 18-mer TAL effector repeats,
which bind to 18-base DNA target sites.
GF
AF
CF
TF
GAACTCTAGC CAGAGTCTT
ACTCTAGC CAGAGTCTTG C
CTCTAGC CAGAGTCTTG CA
TCTAGC CAGAGTCTTG CAT
GAACTCTAGC CAGAGTCTTG CATTTCTCAG TCCTAAACAG GGTAATGGAC TGGGGCTGAA
CTTGAGATCG GTCTCAGAAC GTAAAGAGTC AGGATTTGTC CCATTACCTG ACCCCGACTT
CCATTACCTG ACCCCGACT
C CCATTACCTG ACCCCGAC
TC CCATTACCTG ACCCCGA
GTC CCATTACCTG ACCCCG
TR
CR
AR
GR
Figure 5. Target sites for GeneArt PerfectMatch TALs and GeneArt
Precision TALs at the HPRT locus are shown here as an example of the
similarities between the two targets. The DNA sequences in the pink boxes
represent targeting sequences of TAL effectors. The DNA base in each
light-pink box represents the 5´ base of the TAL effector target sequence.
®
®
TF: TAL effector target site with a 5´ T on forward strand; TR: TAL effector
target site with a 5´ T on reverse strand. CF: TAL effector target site with a
5´ C on forward strand; CR: TAL effector target site with a 5´ C on reverse
strand. GF: TAL effector target site with a 5´ G on forward strand; GR: TAL
effector target site with a 5´ G on reverse strand. AF: TAL effector target
site with a 5´ A on forward strand; AR: TAL effector target site with a 5´ A on
reverse strand.
We compared cleavage efficiencies of GeneArt
PerfectMatch and Precision TALs designed for the
HPRT locus using the GeneArt Genomic Cleavage
Detection Kit, and found GeneArt PerfectMatch TALs
exhibit cleavage efficiencies equal to or better than the
performance of GeneArt Precision TALs on the same
targeted region.
®
®
®
®
®
®
®
®
®
GeneArt PerfectMatch TALs function as well as or
better than current TAL effectors in 293FT and HeLa
cells when the targeting sequences of forward and
reverse TAL effectors are preceded by different (nonidentical) bases (Figure 6).
®
GeneArt PerfectMatch TALs function as well as or
better than current TAL effectors in U2OS cells (Figure
7). GeneArt PerfectMatch TAL pairs with 15–16 bp
spacing were compared with current TAL effectors in 7
loci in U2OS cells.
®
®
A
A
AGCD Enzyme:
GCD Enzyme:
HPRT
RELA
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL CF/AR
control
HPRT
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL CF/AR
control
+ - + - + - + - + -
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL
AF/TR
control
RELA
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL AF/TR
control
+ - + - + - + - + -
ACTB
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL
GF/CR
control
ACTB
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL GF/CR
control
+ - + - + - + - + -
Cleavage 18.1
efficiency (%)
19.2
21.6
25.0
4.9
5.0
16.1
14.3
32.8
37.5
47.6
50.1
Cleavage 18.1
efficiency (%)
19.2
21.6
25.0
4.9
5.0
16.1
14.3
32.8
37.5
47.6
50.1
Figure 6bGeneArt Precision TAL
TF/TR
GCD Enzyme:
Figure
6bGeneArt
+ - Precision
+ TAL
®
®
TF/TR
GCD Enzyme:
CCR5
GeneArt
+ -® PerfectMatch
+ - Negative
+ TAL CF/TR
control
+ - + - + - + - + -
B
Cleavage 9.0
efficiency
B (%) GeneArt
Cleavage
efficiency (%)
IL2
GeneArt® PerfectMatch Negative
TALIL2
CF/TR
control
9.0
®
B
12.6
16.4
16.8
12.6
HPRT
16.4
16.8
Precision TAL GeneArt® PerfectMatch Negative
TAL CF/AR
control
HPRT
TF/TR
GCD Enzyme: GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL CF/AR
control
GCD Enzyme: + - + - + - + - + -
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL
GF/TR
control
CCR5
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL GF/TR
control
+ - + - + - + - + 7.4
10.3
7.4
6.0
7.4
10.3
RELA
7.4
6.0
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL
AF/TR
control
RELA
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL AF/TR
control
+ - + - + - + - + -
IP3R2
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL AF/GR
control
IP3R2
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL AF/GR
control
+ - + - + - + - + 6.6
6.6
13.0
12.9
6.6
6.6
ACTB
13.0
12.9
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL
GF/CR
control
ACTB
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL GF/CR
control
+ - + - + - + - + -
Cleavage 23.6
efficiency (%)
25.3
35.6
35.3
4.5
1.5
12.1
11.1
15.1
14.3
22.0
31.2
Cleavage 23.6
efficiency (%)
25.3
35.6
35.3
4.5
1.5
12.1
11.1
15.1
14.3
22.0
31.2
IL2
CCR5
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TALIL2
CF/TR
control
GCD Enzyme: GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL CF/TR
control
GCD Enzyme: + - + - + - + - + -
Cleavage 22.2
efficiency (%)
21.5
Cleavage 22.2
efficiency (%)
21.5
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL
GF/TR
control
CCR5
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL GF/TR
control
+ - + - + - + - + -
IP3R2
GeneArt® Precision TAL GeneArt® PerfectMatch Negative
TF/TR
TAL AF/GR
control
IP3R2
GeneArt
+ -® Precision
+ TAL
- GeneArt
+ -® PerfectMatch
+ - Negative
+ TF/TR
TAL AF/GR
control
+ - + - + - + - + -
25.9
21.6
9.7
9.8
12.4
11.6
9.4
11.2
15.3
20.7
25.9
21.6
9.7
9.8
12.4
11.6
9.4
11.2
15.3
20.7
Figure 6. GeneArt PerfectMatch TALs function as well as or better than current TAL effectors in 293FT cells (A) and HeLa cells
(B) when targeting sequences of forward and reverse TAL effectors are preceded by different (non-identical) bases. The red
arrowheads point to the cleavage products of the genomic cleavage detection (GCD) enzyme if multiple bands were observed in a
GCD assay.
®
Figure
7
Figure
Figure 7
7
Note: GeneArt PerfectMatch TALs exhibited ≥50% efficiency compared to current TAL effectors on the same targeted region.
®
GeneArt® Precision TAL
GeneArt
TF/TR ® Precision TAL
TF/TR ® Precision TAL
GCD Enzyme: GeneArt
+ - + GCD Enzyme: TF/TR
+ - + GCD Enzyme: + - + -
Cleavage 35.2
Cleavage
efficiency
(%) 35.2
Cleavage
efficiency
(%) 35.2
efficiency (%)
32.6
32.6
32.6
HPRT
HPRT
GeneArt PerfectMatch Negative
HPRT
GeneArt
TAL GF/GRPerfectMatch Negative
control
GeneArt
TAL GF/GRPerfectMatch Negative
+ GF/GR
- + - control
+ TAL
+ - + - control
+ + - + - + ®
®
®
40.0
40.0
40.0
38.0
38.0
38.0
9.1
9.1
9.1
IL2
GeneArt® Precision TAL
GeneArt
TF/TR ® Precision TAL
TF/TR ® Precision TAL
GCD Enzyme: GeneArt
+ - + GCD Enzyme: TF/TR
+ - + GCD Enzyme: + - + -
Cleavage 23.8
Cleavage
efficiency
(%) 23.8
Cleavage
efficiency
(%) 23.8
efficiency (%)
22.0
22.0
22.0
IL2® PerfectMatch
GeneArt
®
GeneArt
PerfectMatch
TALIL2
CF/CR
®
GeneArt
PerfectMatch
TAL CF/CR
+
TAL CF/CR + -
Negative
Negative
control
Negative
control
+ control
+ - + - + + - + - + -
19.7
19.7
19.7
24.4
24.4
24.4
AAVS-1
GeneArt® Precision TAL AAVS-1
GeneArt® PerfectMatch
®
GeneArt
GeneArt
PerfectMatch
TF/TR ® Precision TAL AAVS-1
TAL AF/AR
®
®
GeneArt
Precision
TAL
GeneArt
PerfectMatch
TAL AF/AR
GCD Enzyme: TF/TR
+
+
+
+ TF/TR
TAL
AF/AR
GCD Enzyme: + - + - + - + GCD Enzyme: + - + - + - + -
IP3R2
IP3R2
GeneArt PerfectMatch Negative
IP3R2
GeneArt
TAL CF/ARPerfectMatch Negative
control
GeneArt
TAL CF/ARPerfectMatch Negative
+ CF/AR- + - control
+ TAL
+ - + - + - + - control
+ + - + - + - + - + -
GeneArt® Precision TAL
GeneArt
TF/TR ® Precision TAL
GeneArt
TF/TR ® Precision TAL
+ - + TF/TR
10.2
10.2
10.2
®
®
®
31.5
31.5
31.5
25.2
25.2
25.2
CCR5
GeneArt® Precision TAL
GeneArt
TF/TR ® Precision TAL
GeneArt
TF/TR ® Precision TAL
+ - + TF/TR
®
CCR5
GeneArt
PerfectMatch
®
CCR5
GeneArt
PerfectMatch
TAL
AF/AR
®
GeneArt
PerfectMatch
TAL AF/AR
+ AF/AR- + TAL
16.6
16.6
16.6
11.9
11.9
11.9
+ - + - + - + - + + - + - + - + - + -
17.6
17.6
17.6
21.8
21.8
21.8
20.1
20.1
20.1
8.5
8.5
8.5
GeneArt® Precision TAL
GeneArt
TF/TR ® Precision TAL
GeneArt
TF/TR ® Precision TAL
+ - + TF/TR
®
ACTB
GeneArt
PerfectMatch
®
ACTB
GeneArt
PerfectMatch
TAL
GF/GR
®
GeneArt
PerfectMatch
TAL GF/GR
+ GF/GR
- + TAL
23.3
23.3
23.3
25.2
25.2
25.2
13.8
13.8
13.8
27.0
27.0
27.0
®
®
®
21.6
21.6
21.6
22.8
22.8
22.8
ACTB
Negative
Negative
control
Negative
control
+ control
+ - + - + - + - + + - + - + - + - + 18.5
18.5
18.5
23.4
23.4
23.4
Figure 7. GeneArt PerfectMatch TALs function as well as or better than current TAL effectors in
U2OS cells. GeneArt PerfectMatch TAL pairs with 15–16 bp spacing were compared with current TAL
effectors at 7 loci in U2OS cells. The red arrowheads point to the cleavage products of the genomic
cleavage detection (GCD) enzyme if multiple bands were observed in a GCD assay.
®
Negative
Negative
control
Negative
control
+ control
+ + -
®
Note: “Equal or better function” of GeneArt PerfectMatch TALs is when cleavage efficiency by GeneArt
PerfectMatch TALs is ≥50% efficiency compared to current TAL effector performance on the same
targeted region.
®
Cleavage 11.0
Cleavage
efficiency
(%) 11.0
Cleavage
efficiency
(%) 11.0
efficiency (%)
13.0
13.0
13.0
IP3R1
IP3R1
GeneArt PerfectMatch Negative
IP3R1
GeneArt
TAL TF/ARPerfectMatch Negative
control
GeneArt
TAL TF/ARPerfectMatch Negative
+ TF/AR- + - control
+ TAL
+ - + - + - + - control
+ + - + - + - + - + -
GeneArt® Precision TAL
GeneArt
TF/TR ® Precision TAL
GeneArt
TF/TR ® Precision TAL
+ - + TF/TR
Negative
Negative
control
Negative
control
+ control
®
Conclusion
GeneArt PerfectMatch TALs perform comparably
to or better than GeneArt Precision TALs. GeneArt
PerfectMatch TALs enable precise TAL effector–based
targeting of any DNA sequence—there is no longer a 5´ T
target sequence constraint, allowing you more freedom
to innovate. GeneArt PerfectMatch TALs increase the
flexibility of designing TAL effector targets and make it
possible to keep the spacing distance between targets
of TAL effector pairs at 15–16 bp to get maximal TAL
effector efficiency.
®
®
®
®
Ordering information
The GeneArt PerfectMatch TALs order form is
available at lifetechnologies.com/TALs
®
Place your order by downloading and
completing the order form and emailing it to
geneartsupport@lifetech.com
Related product ordering information
Product
Quantity
Cat. No.
GeneArt Genomic Cleavage Detection Kit
20 reactions
A24372
Lipofectamine 2000 Transfection Reagent
1.5 mL
11668019
Lipofectamine 3000 Transfection Reagent
1.5 mL
L3000015
®
®
®
References
1. Lamb BM, Mercer AC, Barbas CF 3rd (2013) Directed evolution of the TALE N-terminal domain for recognition of all 5´ bases. Nucleic Acids Res 41:9779–9785.
2. Bogdanove AJ, Schornack S, Lahaye T (2010) TAL effectors: finding plant genes for disease and defense. Curr Opin Plant Biol 13:394–401.
3. Bogdanove AJ, Voytas DF (2011) TAL effectors: customizable proteins for DNA targeting. Science 333:1843–1846.
4. Boch J, Scholze H, Schornack S, et al. (2009) Breaking the code of DNA binding specificity of TAL-type III effectors. Science 326:1509–1512.
5. Cermak T, Doyle EL, Christian M, et al. (2011) Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting.
Nucleic Acids Res 39:e82.
6. Mak AN, Bradley P, Cernadas RA, et al. (2012) The crystal structure of TAL effector PthXo1 bound to its DNA target. Science 335:716–719.
Find out more at lifetechnologies.com/tal
For Research Use Only. Not for use in diagnostic procedures. © 2014 Thermo Fisher Scientific Inc. All rights reserved.
All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CO29200 0914