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The Leading Gene Through Screen CRO
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Molecular Biology Services
Molecular Biology drives modern drug discovery. Drug
screening, target validation and structural biology
processes frequently require recombinant DNA reagents
as a starting material.
Although most molecular biology techniques are extremely
common and widely used they can also be time-consuming
and laborious. Moreover, the equipment, labor and facility
investments necessary to perform these activities are
difficult to justify; cost-effective molecular biology services
are readily available in the CRO market.
It is simply more efficient and economical to outsource
these activities. Blue Sky BioServices (BSB) has a team of
highly-trained experts available to deliver quality molecular
biology reagents at a fair price. BSB will support your
molecular biology demands. Our Fee-For-Service model
starts with the initial cloning of your gene(s) of interest, a
flexible process that can accommodate multiparallel
projects (many constructs in parallel). This comprehensive
suite includes (but is not limited to) standard PCR-based
cloning, mutagenesis, construct sequence validation, and
our popular rapid bulk plasmid preparation (TeraPrep™).
BSB leads the industry in customer service excellence.
We rank at the top in client surveys for VALUE attributes:
Quality, Speed, Communication, and Trust at an
affordable Price!
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TeraPrep™ - Reliable Transfection-Grade Plasmid Preps
• 1 mg to 1 GRAM!
• 3-6 Day Turnaround
• High Throughput Multiparallel Capacity
• Premium Transfection Grade Plasmid
Gene Synthesis
• Multiparallel Gene Synthesis with Fast Turnaround Time!
Antibody Sequencing & Cloning
• The Fastest Turnaround of High Quality Antibody Services that Fit Your Budget!
• Cloning of Heavy and Light Chain Variable Regions or Full-length Antibodies,
Co-expression, Purification and Validation of Antigen Binding by ELISA
In addition to these popular services, Blue Sky can assist you with these custom services:
DNA
Viral Preps
• Vector Construction
• Multiparallel Gene Through Protein Services
• Baculovirus
• BacMam
RNA
• qPCR and RT-PCR
• In Vitro: Transcription and Translation
For more information about services not listed, please
contact us to find out what Blue Sky can do for you.
dna@blueskybiotech.com
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Cell Culture and Scale-Up
Protein Expression Services
300 L Capacity. Mammalian & Insect Experts.
Research-based drug discovery increasingly relies on
protein/antibody production using mammalian host cell
lines. In particular demand has grown for fast turnaround
large scale mammalian suspension cell expression.
Blue Sky offers its clients distinctly efficient research-scale
(up to 300 L) mammalian cell expression services.
Blue Sky is widely recognized for its pioneering technologies
and leadership in baculovirus production, baculovirus
amplification, and bioreactor-scale protein expression. The
Insect Expression Team has invested > 2Million USD in
technology improvements of the baculovirus production
and expression process. Blue Sky's industry-leading
baculoviral [insect cell recombinant protein expression]
service utilizes Infection Kinetics Monitoring® [IKM],
a proprietary process technology that significantly
improves turnaround time and reproducibility in
tandem with TIPS/BIIC technology.
100 L Turnaround Time in <12 business days!
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Infection Kinetics Monitoring
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Bacterial, Insect, and Mammalian Scale-Up Services
• Up to 300 L
• Aggressive Timelines at Low Prices!
Antibody Development and Production Services
• Antigen Creation
• Transient and Stable Expression of Recombinant Antibodies
• Hybridomas Adaptation Scale-Up
• ELISA
In addition to these popular services, Blue Sky can assist you with these custom services:
Insect [BEVS] Expression
Mammalian Expression
• Rapid Hi Titer Baculovirus Stock Preparation, with
SDS-PAGE/Western Blot Qualitative QC
• Expression Optimization, 24-96 Data Points on
SDS-PAGE/Western Blot
• Co-inventors of TIPS/BIIC BEVS Technology and Now
Offering BIIC Stock Production and Storage Services
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Bacterial Expression
• Expression Optimization, 24-96 Data Points on
SDS-PAGE/Western Blot
• Metabolic Labeling (In Vivo)
Yeast Expression
• Strain Creation and Optimization
• Intracellular and Secreted Products
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Experience with an Extensive List of Cell Lines
Optimization of Expression in Bioreactors
Flask Scale and Cell Stack Adherent Options
EVA™ (EVolution Accelerated) Rapid Processes for
Adaptation to Suspension and Media Adaptation
Intracellular and Secreted Products
Stable Cell Line Development, Adherent and
Suspension, Pools and Clones
Membrane Preparation
Functional Testing/Viability/Reporter Assay
Virus Preparation and Transfection
BacMam-Mediated Protein Expression
Lentiviral-Mediated Expression
For more information about services not listed, please
contact us to find out what Blue Sky can do for you.
expression@blueskybiotech.com
Meet all of Your Scale-Up Needs with one Partner!
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Protein Science Services
Proteins are indispensable in the early stages of drug
discovery; purified proteins are used for target validation,
enzyme characterization, high-throughput screening
(HTS) and structural studies. In order to study proteins
in vitro, researchers need active protein, free of impurities
that can potentially interfere with mechanistic studies.
Blue Sky's Protein Science Facility understands that
drug discovery is catalyzed by pure, active enzymes that
yield clean signals and reproducible results.
Our Protein Lab is comprised of highly skilled biochemists
with many years of hands-on experience in protein
purification and characterization. Our core expertise in
purification techniques, project management and data
analysis provides the perfect CRO platform for high quality
protein production. We specialize in custom
enrichment/purification method development
[SCOPE™ services] or rapid adaptation of a purification
protocol optimized in our client's lab. Blue Sky has also
optimized a service for endotoxin removal/reduction
and will also test preparations for endotoxin levels
[De-Tox™ services]. We deliver insightful data and
quality protein with world-class turnaround times
to our clients.
TM
SCOPE™
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Bulk Resupply and Antibody Production
• Providing the Fastest Turnaround and Highest Level of Quality for Gram Levels of
Resupply and Antibody Production!
Mulitparallel Enrichments and Method Scouting [SCOPE™]
• Industry Leading Experts in Protein Enrichment and Purification!
• Single Host Strain (Bacterial, Yeast, Insect, Mammalian)
• 12-Point Data Package to Assess Expression and Enrichment
In addition to these popular services, Blue Sky can assist you with these custom services:
Milligram to Gram Levels of Protein Supply!
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Custom Protein Purification
Inclusion Body/Refold Protocols
Membrane Preparations
Microsome Preparations
Virus-Like Particles [VLPs]
Isoelectric Focusing
Subcellular Fractionation
Protein-Protein Interaction Studies
Endotoxin Testing and Removal (De-tox™)
Our laboratories support our clients through
investment in quality
• [ÄKTA] FPLCs for Chromatography
• Affinity
• Ion Exchange
• Hydrophobic Interaction
• Preparative and Analytical Size Exclusion
• Large Volume Precision Cell Disruption/Lysis
• Low and High Speed Centrifugation
• Ultracentrifugation/Fractionation
• Tangential Flow Filtration
• PAGE, Western Blot, Capillary Electrophoresis [CE]
For more information such as services not listed, please
contact us to find out what Blue Sky BioServices can do
for you.
proteins@blueskybiotech.com
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Assay Services
The Assay Services Lab at Blue Sky has extensive
experience with assay development, high throughput
screening (HTS), high content screening (HCS), and
data analysis. We will perform routine and custom
biochemical and cell-based assays using a wide range of
readouts, including radiometric, with a state-of-the-art
compound management and data analysis platform.
• Biochemical Assays
• Cell-based Assays
• Compound Screening
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Radiometric Assays
• Blue Sky Bioservices has a Dedicated Radioactive Lab! We have the Expertise and
Facilities to Assist You with:
• Radioligand Binding Assay
• Radiometric Filter Binding Assay
• SPA Based Assay
• Autoradiography
• Specific Activity Assay
Immunoassays
• ELISA Development or Other Antibody-based Assay Formats
High Content Screening
• Expertise in Imaging Assays, Including Differentiation, Intracellular Trafficking, and Others
In addition to these popular services, Blue Sky can assist you with these custom services:
Biochemical Assays
Compound Libraries
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• Blue Sky's Compound Libraries
• Client's Compound Library
Radiometric
Fluorescence & Luminescence
TDA Screening Platform
Immunoassays (ELISA)
Cell-based Assays
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Reporter Gene
High Content Screening
Phenotypic Drug Discovery
Proliferation and Viability Readout
Compound Screening
• HTS & HCS
• IC50 & EC50 Determination
• Selectivity and SAR Profiling
Target Classes Include
• Membrane Receptors, Membrane-Associated
Proteins, Kinases, Phosphatases, GPCRs, Proteases,
GTP-ases, Epigenetic Targets, PDEs and other
Druggable Targets
Our team of scientists will collaboratively develop an
assay to meet your requirements, including custom
protein production and field application service in your
laboratory, for maximum flexibility.
assays@blueskybiotech.com
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Better leads with TDA 2.0™
Current drug discovery approaches for membraneassociated targets are often ineffective for at least two
reasons. First, the biological context, structure and
organization defined by a membrane are ignored, yet
these properties are critical for relevant pharmacology.
Second, while cell-based assays preserve membrane
context, they are frequently too cumbersome,
time-consuming and costly for discovery programs.
TDA 2.0™ solves these problems!
TDA 2.0™ is designed to confer proper pharmacology by
restoring membrane architecture in a rapid, chemicallydefined assay. TDA 2.0™ delivers the speed of a
biochemical assay with the quality and complexity
of a cell-based assay, allowing identification of
therapeutically-relevant lead compounds.
Blue Sky BioServices offers custom assay development
and screening services using TDA 2.0™ technology.
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TDA assay services:
Selected benefits of TDA™ technology:
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Consultation
Target evaluation
Custom reagent design, development and production
Substrate identification and production
Assay optimization and validation
Compound screening and characterization
Data analysis
Secondary screening and SAR
Study report
Improved enzymatic properties
Assembly of multimeric complexes
Activation of signaling pathways
Recruitment of accessory proteins
Restored substrate recognition
and biochemistry
Contact us now! TDA@blueskybiotech.com
Better leads with TDA 2.0™
TDA2.0™ is a game-changing technology for membrane-associated drug targets that enables development of more
physiologically-relevant assays than traditional biochemical methods. Assays created using TDA exhibit biochemical and
pharmacological profiles that more accurately replicate in vivo activity. Therefore, drug discovery programs utilizing TDA for
membrane-associated therapeutic targets will identify molecules with better efficacy in in vitro and in vivo models and
ultimately enable the development of better drugs. Blue Sky Bioservices owns exclusive rights to TDA technology for drug
discovery applications
TDA technology covered under US 7678540 and US 8268965 patents. Other US and foreign patents pending.
Please contact Blue Sky BioServices for licensing terms and conditions.
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Blue Sky provides a faster path to productivity.
Make More Time. Relieve Bottlenecks. Focus On Innovation.
Blue Sky BioServices is the leading Contract Research Organization (CRO) providing Gene
Through Screen services in the Biotechnology R&D market. Our clients enhance the quality
and speed of their research by enlisting Blue Sky's premium services such as vector/gene
construction, recombinant protein expression, laboratory-scale fermentation (scale-up
bioprocessing), protein purification, assay development, and assay execution (screening).
Blue Sky is not your average CRO and invests heavily in experienced people, leading edge
technology, and state-of-the-art laboratory facilities. This investment in service platforms and
scientists sets Blue Sky apart from other CROs and transforms the company into a center of
excellence with unmatched quality and turnaround times.
Our suite of services is our client's Pathway to Productivity. We are motivated by our ability to
impact the overall efficiency of the drug discovery process and thus contributing to a longer,
healthier lifespan for human beings.
Blue Sky BioServices
50 Prescott Street, Worcester, Massachusetts, 01605-2661 USA
800-383-7795
www.blueskybioservices.com experts@blueskybiotech.com